Tissue Dissociation References


	Tissue Dissociation References
	Tissue Type: Aorta

Aorta: Bovine
Bovine
	Warshaw, D., Szarek, J., Hubbard, M., and Evans, J.: Pharmacology and Force Development of Single Freshly Isolated Bovine Cartoid Artery Smooth Muscle Cells, Circ Res 58, 399, 1986
Cells:	Vascular smooth muscle cells
Enzyme(s)	Collagenase Type I: 500 U/ml Elastase Type III: 50 U/ml Deoxyribonuclease: 60 U/ml
Medium	PSS
Aorta: Human
Human
	Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8), 713, 1981
Cells:	Smooth muscle cells
Enzyme(s)	Collagenase: 10 ml 0.1% Elastase: 0.05% Trypsin: 0.25%
Medium	DMEM
Aorta: Rat
Male, SD
	Cornwell, T., and Lincoln, T.: Regulation of Intracellular Ca²⁺ Levels in Cultured Vascular Smooth Muscle Cells, J Biol Chem 264 (2), 1146, 1989
Cells:	Aortic smooth muscle cells
Enzyme(s)	Collagenase Type III: 200 units/ml Elastase: 0.1 mg Soybean Trypsin Inhibitor: 0.5 mg/ml
Medium	DMEM
Aorta: Rat
Male rats, 150-250 g
	Cole, O., Fan, T., and Lewis, G.: Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta, Cell Biol Int Rep 10 (6), 399, 1986
Cells:	Endothelial cells
Enzyme(s)	Collagenase Dispase II Trypsin Elastase Hyaluronidase
Medium	RPMI 1640
Aorta: Rat
Either sex, 12-19 days, 3 momths, and retired breeders, SHR, WKY
	Gordon, D., Mohai, L., and Schwartz, S.: Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells, Circ Res 59, 633, 1986
Cells:	Endothelial cells
Enzyme(s)	Collagenase Type I: 0.1% Elastase: 0.05%
Medium	Waymouth's culture medium