Tissue Dissociation References
Tissue Type: Aorta

Aorta: Bovine
  • Bovine
Warshaw, D., Szarek, J., Hubbard, M., and Evans, J.: Pharmacology and Force Development of Single Freshly Isolated Bovine Cartoid Artery Smooth Muscle Cells, Circ Res 58, 399, 1986
Cells:     Vascular smooth muscle cells
Enzyme(s)    Collagenase Type I: 500 U/ml
Elastase Type III: 50 U/ml
Deoxyribonuclease: 60 U/ml
Medium    PSS

Aorta: Human
  • Human
Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8), 713, 1981
Cells:     Smooth muscle cells
Enzyme(s)    Collagenase: 10 ml 0.1%
Elastase: 0.05%
Trypsin: 0.25%
Medium    DMEM

Aorta: Rat
  • Male, SD
Cornwell, T., and Lincoln, T.: Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells, J Biol Chem 264 (2), 1146, 1989
Cells:     Aortic smooth muscle cells
Enzyme(s)    Collagenase Type III: 200 units/ml
Elastase: 0.1 mg
Soybean Trypsin Inhibitor: 0.5 mg/ml
Medium    DMEM

Aorta: Rat
  • Male rats, 150-250 g
Cole, O., Fan, T., and Lewis, G.: Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta, Cell Biol Int Rep 10 (6), 399, 1986
Cells:     Endothelial cells
Enzyme(s)    Collagenase
Dispase II
Medium    RPMI 1640

Aorta: Rat
  • Either sex, 12-19 days, 3 momths, and retired breeders, SHR, WKY
Gordon, D., Mohai, L., and Schwartz, S.: Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells, Circ Res 59, 633, 1986
Cells:     Endothelial cells
Enzyme(s)    Collagenase Type I: 0.1%
Elastase: 0.05%
Medium    Waymouth's culture medium

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