Tissue Dissociation References


	Tissue Dissociation References
	Tissue Type: Blood

Blood: Human
Fifteen patients, ages 6-58 yrs.
	Pastorelli, G., Roncarolo, M., Touraine, J., Peronne, G., Tovo, P., and Vries, J.: Peripheral Blood Lymphocytes of Patients With Common Variable Immunodeficiency (CVI) Produce Reduced Levels of Interleukin-4, Interleukin-2 and Interferon-Gamma, but Proliferate Normally Upon Activation by Mitogens, Clin Exp Immunol 78, 334, 1989
Cells:	Peripheral Blood Lymphocytes
Enzyme(s)	Alkaline Phosphatase
Medium	RPMI 1640
Blood: Human
HIV positive humans
	Production of Tumour Necrosis Factor-Alpha (TNF-a) and Interleukin-1 (IL-1) in Patients with AIDS. Enhanced Level of TNF-a is Related to a Higher Cytotoxic Activity: , Clin Exp Immunol 78, 329, 1989
Cells:	Mononuclear cells
Enzyme(s)
Medium	RPMI 1640
Blood: Human
Human
	Scanlon, M., Williams, D., and Fay, F.: A Ca2+-Insensitive Form of Fura -2 Associated with Polymorphonuclear Leukocytes, J Biol Chem 262 (13), 6308, 1987
Cells:	Polymorphnuclear leukocytes
Enzyme(s)
Medium	HBSS
Blood: Human
Human
	Traganos, F., Gorski, A., Darzynkiewicz, Z., Sharpless, T., and Melamed, M.: Rapid Multiparameter Analysis of Cell Stimulation in Mixed Lymphocyte Culture Reactions, J Histochem Cytochem 25 (7), 881, 1977
Cells:	Lymphocytes
Enzyme(s)	Ribonuclease A: 1000 units/ml Deoxyribonuclease: 1000 units/ml
Medium	HBSS
Blood: Human
Human
	Darzynkiewicz, Z., Traganos, F., Sharpless, T., and Melamed, M.: Lymphocyte Stimulation: A Rapid Multiparameter Analysis, Proc Natl Acad Sci U S A 73 (8), 2881, 1976
Cells:	Mononuclear cells
Enzyme(s)
Medium	(see reference)