Bone - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine Chondrocytes Collagenase Type 2: 0.4%
 DMEM Buschmann, M., Gluzband, Y., Grodzinsky, A., and Hunziker, E.: Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture, J Cell Sci 108, 1497, 1995
Chicken Chick, day old Osteoblasts Trypsin: 0.03%
 DMEM Gay, C., Lloyd, Q., and Gilman, V.: Characteristics and Culture of Osteoblasts Derived From Avian Long Bone, In Vitro Cell Dev Biol 30A, 379, 1994
Chicken Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus) Chondrocytes Trypsin: 0.25%
 Ham's F12 Rosselot, G., Reginato, A.M., and Leach, R.M.: Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes, In Vitro Cell Dev Biol 28A, 235, 1992
Chicken Chick, embryo Vertebrae chondroblasts Trypsin: 0.25%
 Simm's, CMF Schiltz, J.R., Mayne R., and Holtzer, H.: The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture, Differentiation 1, 97, 1973
Human Human Bone-cartilage-stromal Collagenase Type 2: 0.75%
 HBSS Agarwal, S., Loder, S., Sorkin, M., Li, S., Shrestha, S., Zhao, B., Mishina, Y., James, A. and Levi, B.: Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification., Stem Cells 34, 1692-701, 2016
Human Human Osteoblasts Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
 DMEM Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts., Bonekey Rep 3, 585, 2014
Human Human Osteoblasts Collagenase Type 3: 0.2%
 MEM Kode, A.,Manavalan1, J.,Mosialou1, I., Bhagat G., Rathinam, C, Luo, N et al.: Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts, Nature 506, 240, 2014
Human Human, 22-73 year Osteoblasts Trypsin: 0.5%
 Basal Medium Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ: Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute, Cells Tissues Organs 179, 158-69, 2005
Human Human, 60+ year Bone Cells, Osteoblasts Collagenase Type 2: 200-250 u/ml
 DMEM Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage, J Bone Miner Res 14, 362, 1999
Human Human Osteoblasts Trypsin: 0.1%
 DMEM Meikle, M., Boyd, S., Hembry, R., Compston, J., Croucher, P., and Reynolds, J.: Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines, J Cell Sci 103, 1093, 1992
Human Human Osteoblasts Collagenase Type 4: 250 u/ml
 DMEM Fedarko, N.S., Termine, J.D., Young, M.F. and Robey, P.G.: Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro, J Biol Chem 265, 12200, 1990
Mouse Mouse Bone and bone marrow stromal Stemxyme 1: 0.1%
Neutral Protease: 0.1%
 PBS Mohrin, D., Koukos, G., Alabdulaaly, L., Paw, J., Hake, K., Kolumam, G., Bouxsein, M., Baron, R., Kutskova, Y., Freund, A., Liu, J., Zavala-Solorio, J., Bhargava, S., Maxwell, Trumble, J., Brito, A., Hu, D. and Brooks, D.: Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells.,Aging Cell Vol. 20,, , e13313, 2021
Mouse Mouse Bone and bone marrow stroma Stemxyme 1: 0.1%
Neutral Protease: 0.1%
 Media 199 Baryawno, N., Przybylski, D., Kowalczyk, M., Kfoury, Y., Severe, N., Gustafsson, K., Kokkaliaris, K., Mercier, F., Tabaka, M., Hofree, M., Dionne, D., Papazian, A., Lee, D., Ashenberg, O. and Subramanian, A.: A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia., Cell 177, 1915-1932.e16, 2019
Mouse Mouse Osteoblast Collagenase Type 1: 0.3%
 HBSS Wang, W., Majihail, G., Lui, C. and Zhou, L.: Osteoblast Sorting and Intracellular Staining of CXCL12., Bio Protoc 8, , 2018
Mouse Mouse Bone-cartilage-stromal Collagenase Type 2: 0.75%
 HBSS Agarwal, S., Loder, S., Sorkin, M., Li, S., Shrestha, S., Zhao, B., Mishina, Y., James, A. and Levi, B.: Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification., Stem Cells 34, 1692-701, 2016
Mouse Mouse Bone stromal Collagenase Type 1: 0.3%
 DMEM Igarashi, H., Akahoshi, N., Ohto-Nakanishi, T., Yasuda, D. and Ishii, S.: The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells., Sci Rep 5, 11410, 2015
Mouse Mouse Osteoblasts Collagenase Type 1: 1.0%
Neutral Protease: 1.6%
 MEM Lee, D., Choung, H., Kim, H., Gronostajski, R., Yang, Y., Ryoo, H., Lee, Z., Kim, H., Cho, E. and Park, J.: NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression., Stem Cells 32, 2467-79, 2014
Mouse Mouse, 2-5 day Osteoblasts Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
 DMEM Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts., Bonekey Rep 3, 585, 2014
Mouse Mouse Osteoblasts Collagenase Type 3: 0.2%
 MEM Kode, A.,Manavalan1, J.,Mosialou1, I., Bhagat G., Rathinam, C, Luo, N et al.: Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts, Nature 506, 240, 2014
Mouse Mouse, 4-22 month Osteocytes Collagenase Type 1: 300 u/ml
 MEM Stern, A., Stern, M., Van Dyke, M., Jahn, K., Prideaux, M. and Bonewald, L.: Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice., Biotechniques 52, 361, 2012
Mouse Mouse Endosteal cells Collagenase Type 1: 0.3%
 DMEM Nakamura, Y., Arai, F., Iwasaki, H., Hosokawa, K., Kobayashi, I., Gomei, Y., Matsumoto, Y., Yoshihara, H. and Suda, T.: Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells., Blood 116, 1422, 2010
Mouse Mouse Bone marrow Collagenase: 0.2%
 DMEM Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H. and Matsuzaki, Y.: Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow., J Exp Med 206, 2483-96, 2009
Mouse Mouse, male, 6-8 week old Osteoclasts Collagenase Type 3: 0.1%
 DMEM Sakai, E., Miyamoto, H., Okamoto, K., Kato, Y., Yamamoto, K., and Sakai, H.: Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages, J. Biochem. 130, 823, 2001
Mouse Mouse, 3-5 day, 6-8 week Bone Cells, Osteoblasts Collagenase Type 2: 200-250 u/ml
 DMEM Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage, J Bone Miner Res 14, 362, 1999
Mouse Mouse, BALB/c Osteoblast-like Cells
Stromal Cell Lines
Hematopoietic Blast Cells		 Trypsin: 0.1%
 Eagle's MEM Takanashi, H., Matsuishi, T., and Yoshizato, K.: Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells, In Vitro Cell Dev Biol 30A, 384, 1994
Mouse Mouse, Swiss-Webster Neonatal bone Collagenase Type 2: 0.20%
 Tris-buffered saline Chen,T. and Feldman,D.: Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells, J Biol Chem 256, 5561, 1981
Rat Rat, 2 day Osteoblasts Collagenase Type 2: 0.1%
Trypsin: 0.25%
 DMEM Jeon, J., Lee, M. and Yang, H.: Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation., Biomater Res 22, 4, 2018
Rat Rat, Wistar, 250g Proximal femur stem Collagenase Type 1: 0.075%
 HBSS Jacobs, F., Gijsen, H., Van de Vyver, M. and Ferris, W.: Vanadate Impedes Adipogenesis in Mesenchymal Stem Cells Derived from Different Depots within Bone., Front Endocrinol 7, 108, 2016
Rat Rat, 2-3 day Osteoblasts Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
 DMEM Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts., Bonekey Rep 3, 585, 2014
Rat Rat, fetal, 21 days of gestation Calvaria Collagenase: 0.2%
 MEM Owen, T., Aronow, M., Shalhoub, V., Barone, L., Wilming, L., Tassinari, M., Kennedy, M., Pockwinse, S., Lian, J., and Stein, G.: Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix, J Cell Physiol 143, 420, 1990
Rat Rat, newborn Osteoblastlike cells Collagenase Type 2: 0.3%
 MEM Ernst, M., and Froesch, E.: Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I, Calcif Tissue Int 40, 27, 1987
Rat Rat, fetus, 17-21 day Calvaria Collagenase: 0.01%-0.6%
 Tris-buffered saline Peck, W., Birge, S., and Fedak, S.: Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation, Science 146, 1476, 1964