Tissue Dissociation References


	Tissue Dissociation References
	Tissue Type: Calvaria

Calvaria: Mouse
BALB/c mice
	Takanashi, H., Matsuishi, T., and Yoshizato, K.: Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells, In Vitro Cell Dev Biol 30A, 384, 1994
Cells:	Osteoblastlike cellsStromal cell linesHematopoietic blast cells
Enzyme(s)	Collagenase Type I: 0.2% Trypsin: 0.1%
Medium	Eagle's MEM
Calvaria: Rat
Newborn rats
	Ernst, M., and Froesch, E.: Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I, Calcif Tissue Int 40, 27, 1987
Cells:	Osteoblastlike cells
Enzyme(s)	Collagenase Type II: 3 mg/ml
Medium	MEM