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Tissue Dissociation References
Tissue Type: Embryo


Embryo: Chicken
  • HH stage 23-24 chick embryos
Wong, M., and Tuan, R.: Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture, In Vitro Cell Dev Biol 29A, 917, 1993
Cells:     Mesenchymal cells
Enzyme(s)    Collagenase Type II: 1 mg/ml
Trypsin: 1 mg/ml
Medium    DMEM(see reference)


Embryo: Chicken
  • 19 day old White Leghorn chick embryos
Gionti, E., Capasso, O., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro, J Biol Chem 258 (11), 7190, 1983
Cells:     FibroblastsEpithelial-like cells
Enzyme(s)    Trypsin: 0.25%
Collagenase Type II: 100 units/ml
Medium    E 199 medium


Embryo: Chicken
  • 19 day old chick embryos
Capasso, O., Gionti, E., Pontarelli, G., Ambesi-Impiobato, F., Nitsch, L., Tajana, G., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro, Exp Cell Res 142, 197, 1982
Cells:     Chondrocytes
Enzyme(s)    Collagenase Type I: 7 U/ml
Collagenase Type II: 100 U/ml
Trypsin: 0.75 mg/ml
Medium    Coon's modified F-12


Embryo: Chicken
  • White Leghorn chick embryos
Ahrens, P., Solursh, M., and Reiter, R.: Stage-Related Capacity for Limb Chondrogenesis in Cell Culture, Dev Biol 60, 69, 1977
Cells:     Wing buds
Enzyme(s)    Trypsin: 0.1%
Collagenase: 0.1%
Medium    Saline G


Embryo: Mouse
  • 17-19 day old Swiss mouse embryos
Todaro, G., and Green, H.: Quantitative Studies of the Growth of Mouse Embryo Cells in Culture and Their Development into Established Lines, J Cell Biol 17, 299, 1963
Cells:     Mouse embryo cells
Enzyme(s)    Trypsin: 0.25%
Medium    DMEM

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