Tissue Dissociation References
Tissue Type: Endothelium

Endothelium: Mouse
  • BALB/c, CBA, SJL/J mice, 2-4 wks old
Sapatino, B., Welsh, C., Smith, C., Bebo, B., and Linticum, D.: Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme, In Vitro Cell Dev Biol 29A, 923, 1993
Cells:     Cerebrovascular endothelial cells
Enzyme(s)    Collagenase: 0.1%
Medium    PBS

Endothelium: Mouse
  • Weanling & young adult Swiss-Webster mice
DeBault, L., Henriquez, E., Hart, M., and Cancilla, P.: Cerebral Microvessels and Derived Cells in Tissue Culture, In Vitro 17 (6), 480, 1981
Cells:     Cerebral microvessels
Enzyme(s)    Trypsin: 0.25%
Medium    Eagle's MEM(see reference)

Endothelium: Porcine
  • Porcine
Shasby, S.: Endothelial Cells Grown On Permeable Membrane Supports, J Tiss Cul Meth 14, 247, 1992
Cells:     Endothelial cells
Enzyme(s)    Trypsin: 0.25%
Collagenase: 2 mg/ml
Medium    Medium 199

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