Tissue Dissociation References


	Tissue Dissociation References
	Tissue Type: Endothelium

Endothelium: Mouse
BALB/c, CBA, SJL/J mice, 2-4 wks old
	Sapatino, B., Welsh, C., Smith, C., Bebo, B., and Linticum, D.: Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme, In Vitro Cell Dev Biol 29A, 923, 1993
Cells:	Cerebrovascular endothelial cells
Enzyme(s)	Collagenase: 0.1% Dispase
Medium	PBS
Endothelium: Mouse
Weanling & young adult Swiss-Webster mice
	DeBault, L., Henriquez, E., Hart, M., and Cancilla, P.: Cerebral Microvessels and Derived Cells in Tissue Culture, In Vitro 17 (6), 480, 1981
Cells:	Cerebral microvessels
Enzyme(s)	Trypsin: 0.25%
Medium	Eagle's MEM(see reference)
Endothelium: Porcine
Porcine
	Shasby, S.: Endothelial Cells Grown On Permeable Membrane Supports, J Tiss Cul Meth 14, 247, 1992
Cells:	Endothelial cells
Enzyme(s)	Trypsin: 0.25% Collagenase: 2 mg/ml
Medium	Medium 199