Kidney - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Avian Avian, house sparrows Tubule segments Collagenase Type 2: 0.1%
 HBSS Goldstein D., Reddy V., and Plaga K.: Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones, Am J Physiol 276, R847, 1999
Avian Avian, chicken Tubule and glomeruli Collagenase Type 1: 0.1%
 RPMI 1640 Reich, C. and Bonar, R.: Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture, Meth Cell Sci 7, 97, 1982
Bovine Bovine Papillary duct Hyaluronidase: 0.2%
 Keri's buffer
HEPES buffered saline		 Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255, F1160, 1988
Canine Canine Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
Canine Dog, 1-5 months Proximal tubules Collagenase Type 1: 0.15%
 Krebs Ringer bicarbonate buffer States, B., Reynolds, R., Lee, J., and Segal, S.: Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments, In Vitro Cell Dev Biol 26, 105, 1990
Canine Dog, mongrel, 20 Kg Proximal tubular Deoxyribonuclease I: 0.0125%
 (see reference) Yau, C., Rao, L., and Silverman, M.: Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells, Can J Physiol Pharmacol 63, 417, 1985
Fish Winter flounder (Pseudopleur-onectes americanus)
200 - 500 g		 Renal tubule Trypsin: 0.2%
Trypsin: 0.2%
 CMF solution Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport, Am J Physiol 251, 424, 1986
Guinea-Pig Guinea-pig, male, 100 g Single cells Trypsin: 0.25%
 CF salt solution Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase, In Vitro 8, 101, 1972
Hamster Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)		 Papillary duct Hyaluronidase: 0.2%
 Keri's buffer
HEPES buffered saline		 Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255, F1160, 1988
Human Human Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
Human Human, adult Renal tumor cells and proximal tubular epithelial Collagenase Type 2: 0.1%
 DMEM/F12 Valente, M., Henrique, R., Costa, V., Jeronimo, C., Carvalho, F., Bastos, M., Guedes de Pinho, P. and Carvalho, M.: A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens., PLoS ONE 6, e19337, 2011
Human Human Renal cells Collagenase Type 4: 300 u/ml
Neutral Protease: see reference
 HBSS Presnell, S., Bruce, A., Wallace, S., Choudhury, S., Genheimer, C., Cox, B., Guthrie, K., Werdin, E., Tatsumi-Ficht, P., Ilagan, R., Kelley, R., Rivera, E., Ludlow, J., Wagner, B., Jayo, M. and Bertram, T.: Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys., Tiss Eng 17, 261, 2011
Human Human, adult Renal proximal tubule and cortical fibroblasts Collagenase Type 2: 383 u/ml
 DMEM/F-12 Johnson, D., Saunders, H., Johnson, F., Huq, S., Field, M., and Pollock, C.: Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor, J Pharmacol Exp Ther 289, 535-42, 1999
Human Human Renal cortex Trypsin: 0.1%
 Tissue Culture Grade Water McAteer, J, Kempson, S., and Evan, A: Culture of Human Renal Cortex Epithelial Cells, J Tiss Cul Meth 13, 143, 1991
Human Human Papillary duct Collagenase: 400 u/ml
 Eagle's MEM-HEPES buffer w/L-glutamine Trifillis, A. and Kahng, M.: Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells, In Vitro Cell Dev Biol 26, 441, 1990
Human Human Mesangial Trypsin: 0.25%
 DMEM/Ham's F-12 Heieren, M., van der Woude, F., and Balfour Jr., H.: Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells, Proc Natl Acad Sci U S A 85, 1642, 1988
Human Human, adult Tubular Collagenase: 250 u/ml
 PBS Yang, A., Gould-Kostka, J., and Oberley, T.: In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate, In Vitro Cell Dev Biol 23 (1), 34, 1987
Human Human, adult, 14-66 years Tubular Collagenase: 100 u/ml
 Joklik's MEM Trifillis, A., Regec, A., and Trump, B.: Isolation, Culture, and Charaterization of Human Renal Tubular Cells, J Urol 133, 324, 1985
Human Human Malignant
Stromal		 Papain: 0.009%
 Sacks solution Hemstreet, G., Enoch, P., and Pretlow, T.: Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification, Cancer Res 40, 1043, 1980
Human Human, 24 newborn and stillborn
(also rabbit)		 Renal Trypsin: 0.25%
 See reference De Oca, H., and Malinin, T.: Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys, J Clin Microbiol 2, 243, 1975
Monkey Monkey Kidney Trypsin: 0.01% - 0.00001%
 Eagle's MEM Melnick, J., and Wallis, C.: Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells, Dev Biol 37, 77, 1976
Mouse Mouse Mactrophages Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.01%
 DMEM Yuan, Y., Li, L., Zhu, L., Liu, F., Tang, X., Liao, G., Liu, J., Cheng, J., Chen, Y. and Lu, Y.: Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy., Stem Cells 38, 639-652, 2020
Mouse Mouse Kidney Deoxyribonuclease I: 125 u/ml
Protease, Bacillus Licheniformis: 1%
 DPBS Adam, M., Potter, A. and Potter, S.: Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development., Development 144, 3625-3632, 2017
Mouse Mouse Collecting duct epithelial Collagenase Type 2: 0.1%
 DMEM DiRocco, D., Kobayashi, A., Taketo, M., McMahon, A. and Humphreys, B.: Wnt4/β-catenin Signaling in Medullary Kidney Myofibroblasts., J Am Soc Nephrol 24, 1399, 2013
Mouse Mouse Glomeruli Collagenase Type 4: 0.1%
 RPMI 1640 Kabgani, N., Grigoleit, T., Schulte, K., Sechi, A., Sauer-Lehnen, S., Tag, C., Boor, P., Kuppe, C., Warsow, G., Schordan, S., Mostertz, J., Chilukoti, R., Homuth, G., Endlich, N., Tacke, F. and Weiskirchen, R.: Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin., PLoS ONE 7, e34907, 2012
Mouse Mouse Glomeruli Collagenase Type 1: 0.1%
Deoxyribonuclease I: 100 u/ml
 DMEM Sedrakyan, S., Da Sacco, S., Milanesi, A., Shiri, L., Petrosyan, A., Varimezova, R., Warburton, D., Lemley, K., De Filippo, R. and Perin, L.: Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis., J Am Soc Nephrol 23, 661-73, 2012
Mouse Mouse, 10-14 week Proximal tubule Collagenase Type 1: 0.1%
 See reference Park, J., Pasupulati, R., Feldkamp, T., Roeser, N. and Weinberg, J.: Cyclophilin D and the Mitochondrial Permeability Transition in Kidney Proximal Tubules after Hypoxic and Ischemic Injury., Am J Physiol/Renal 301, F134, 2011
Mouse Mouse, embryonic Kidney Collagenase Type 1: 0.25%
Pancreatin: 1.0%
Deoxyribonuclease I: 1 u/ml
 HBSS Brown, A., Blank, U., Adams, D., Karolak, M., Fetting, J., Hill, B. and Oxburgh, L.: Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney., J Vis Exp 50, e2555, 2011
Mouse Mouse, adult Kidney sphere-derived Collagenase Type 4: 0.1%
 Iscove's Modified Dulbecco's Huang, Y., Johnston, P., Zhang, B., Zakari, A., Chowdhry, T., Smith, R., Marban, E., Rabb, H. and Womer, K.: Kidney-Derived Stromal Cells Modulate Dendritic and T Cell Responses., J Am Soc Nephrol 20, 831-41, 2009
Mouse Mouse, male, 8-16 week Renal tubular epithelial Collagenase: 200 u/ml
Soybean Trypsin Inhibitor: see reference
 HBSS Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress., Oxid Med Cell Longev 1, 33, 2008
Mouse Mouse Proximal tubule Collagenase Type 2: 0.013%
 see reference Wright, J., Morales, M., Sousa-Menzes, J., Ornellas, D., Sipes, J., Cui, Y., Cui, I., Hulamm, P., Cebotaru, V., Cebotaru, L., Guggino, W. and Guggino, S.: Transcriptional Adaptation to Clcn5 Knockout in Proximal Tubules of Mouse Kidney., Physiol Genomics 33, 341, 2008
Mouse Mouse, 4 week Endothelial kidney Collagenase Type 1: 0.1%
 DMEM Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis., Am J Physiol Cell Physiol 292, C2070, 2007
Mouse Mouse, adult Cortex, proximal tubule Collagenase: 0.15%
 DMEM Syal Ashu, Schiavi Susan, Chakravarty Sumana, Dwarakanath Vangipuram, Quigley Raymond, Baum Michel: Fibroblast growth factor-23 increases mouse PGE2 production in vivo and in vitro, Am J Physiol/Renal 290, F450-5, 2006
Mouse Mouse Kidney mesenchymal Collagenase: 250 u/ml
Neutral Protease: 33.3 u/ml
 DMEM/F12 Plotkin, M. and Goligorsky, M.: Mesenchymal Cells from Adult Kidney Support Angiogenesis and Differentiate into Multiple Interstitial Cell Types including Erythropoietin-Producing Fibroblasts., Am J Physiol Renal Physiol 291, F902-12, 2006
Mouse Mouse, 10-12 week Kidney Collagenase Type 4: 0.075%
 DMEM Dekel, B., Zangi, L., Shezen, E., Reich-Zeliger, S., Eventov-Friedman, S., Katchman, H., Jacob-Hirsch, J., Amariglio, N., Rechavi, G., Margalit, R. and Reisner, Y.: Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney., J Am Soc Nephrol 17, 3300, 2006
Mouse Mouse, male, 12-16 week Renal proximal tubule cells Collagenase Type 2: 0.1%
Soybean Trypsin Inhibitor: 0.25%
 DMEM/F-12 Cunningham, R., Xiaofei, E., Steplock, D., Shenolikar, S. and Weinman, E.: Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media., Am J Physiol Renal Physiol 289, F933, 2005
Mouse Mouse, male, 2 month Cortical collecting duct Collagenase: 54-178 u/ml
Protease: see reference
 MEM Sindic, A., Velic, A., Basoglu, C., Hirsch, J., Edemir, B., Kuhn, M. and Schlatter, E.: Uroguanylin and Guanylin Regulate Transport of Mouse Cortical Collecting Duct Independent of Guanylate Cyclase C., Kidney Int 68, 1008, 2005
Mouse Mouse, 6 week Glomerular mesangial cells Collagenase Type 4: 0.1%
 RPMI 1640 Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE.: Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis, J Biol Chem 277(30), 27535, 2002
Mouse Mouse, 3-7 month Fibroblasts, mesangial, smooth muscle Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: 0.05%
 DMEM Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation, Mol Biol Cell 10, 1569-79, 1999
Mouse Mouse Epithelial Collagenase Type 4: 1%
 DMEM /F-12 Taub, M: Renal Tubule Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley & Sons, Ltd., 14B:6.1, 1995
Mouse Mouse, naive SJL (H-2S) Proximal tubular epithelial Deoxyribonuclease I: 15 µg/ml
 RPMI 1640 Haverty, T., Kelly, C., Hines, W., Amenta, P., Watanabe, M., Harper, R., Kefalides, N., and Neilson, E.: Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis, J Cell Biol 107, 1359, 1988
Porcine Porcine, hybrid Kidney Collagenase Type 1: 0.1%
 HBSS Heussner, A. and Dietrich, D.: Primary Porcine Proximal Tubular Cells as an Alternative to Human Primary Renal Cells in Vitro: an Initial Characterization., BMC Cell Biol 14, 55, 2013
Rabbit Rabbit Renal proximal tubular Collagenase Type 1: 60 u/ml
 DMEM/F12 Nowak, G. and Bakajsova, D.: Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes., J Vis Exp 71, e4301, 2013
Rabbit Rabbit, New Zealand Proximal tubule cells Collagenase Type 4: 0.005%
Soybean Trypsin Inhibitor: 0.00025%
 DMEM/F-12 Taub, M.: Primary Kidney Proximal Tubule Cells., Methods Mol Biol 290, 231, 2005
Rabbit Rabbit, New Zealand White, female Nephron segments Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
 Eagle's MEM Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments, Am J Physiol 273, F650, 1997
Rabbit Rabbit, New Zealand white, male, 1.5 - 2.0 Kg Duct Soybean Trypsin Inhibitor: 0.025%
 Hank's Solution with calcium and HEPES Naray-Fejes-Toth, A., and Fejes-Toth, G.: Immunoelection and Culture of Cortical Collecting Duct Cells, J Tiss Cul Meth 13, 179, 1991
Rabbit Rabbit, New Zealand white, female, 2-3 Kg Renal proximal tubules Deoxyribonuclease I: 70 u/ml
 Modified DME-F12 Rodeheaver, D., Aleo, M., and Schnellmann, R.: Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation, In Vitro Cell Dev Biol 26, 898, 1990
Rabbit Rabbit
(also rat, SD and Wistar, 150-225 g, hamster, bovine)		 Papillary duct Hyaluronidase: 0.2%
 Keri's buffer
HEPES buffered saline		 Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255, F1160, 1988
Rabbit Rabbit, New Zealand, white, 2-3 kg MTALH cells
RCCT cells		 Collagenase: 0.1%
 DMEM Allen, M., Nakao, A., Sonnenburg, W., Burnatowska-Hledin, M., Speilman, W., and Smith, W.: Immunodissection of Cortical and Medullary Thick Ascending Limb Cells From Rabbit Kidney, Am J Physiol 255, F704, 1988
Rabbit Rabbit, New Zealand, white Collecting tubule Trypsin: 0.05%
 Kreb's Ringer buffer Grenier, F., and Smith, W.: Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae, Prostaglandins 16, 759, 1978
Rabbit Rabbit, New Zealand, white, female, 2.5 Kg Papillary collecting duct Collagenase: 0.2%
 (see reference) Dworzack, D., and Grantham, J.: Preparation of Renal Papillary Collecting Duct Cells for Study, Nat Neurosci 8, 191, 1975
Rat Rat, SD, fetal Fetal kidney Collagenase Type 4: 0.1%
 MEM Gupta, A., Jadhav, S., Tripathy, N. and Nityanand, S.: Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects., PLoS ONE 10, e0131057, 2015
Rat Rat, Lewis, male Renal adipose derived cells Collagenase Type 1: 0.3%
 DMEM Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
Rat Rat Proximal tubule Collagenase Type 2: 0.1%
 see reference Panico, C., Luo, Z., Damiano, S., Artigiano, F., Gill, P. and Welch, W.: Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3., Hypertension 54, 1291, 2009
Rat Rat, SD, male, 200-250g Medullary thick ascending limb Collagenase: 0.1%
 HEPES-saline Silva, G., Beierwaltes, W., and Garvin, J.: Extracellular ATP stimulates NO production in rat thick ascending limb, Hypertension 47, 563-7, 2006
Rat Rat, Wistar, 300g Renal proximal tubules Collagenase Type 2: 0.2%
 DMEM/F12 Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C: Kidney oxygen consumption, carbonic anhydrase, and proton secretion, Am J Physiol/Renal 290, F1009-15, 2006
Rat Rat, Wistar, male, 300-350 g Renal proximal tubules Collagenase Type 2: 0.2%
 DMEM/F-12 Deng, A., Miracle, C., Suarez, J., Lortie, M., Satriano, J., Thomson, S., Munger, K. and Blantz, R.: Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II., Kidney Int 68, 723, 2005
Rat Rat Cortical tubule cells Collagenase: 0.6%
 DMEM Arystarkhova, E., Wetzel R., and Sweadner K.: Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney, Am J Physiol/Renal 282(3), F393, 2002
Rat Rat, SD, male 275-300 g Glomerular mesangial cells Collagenase Type 1: 250 u/ml
 DMEM Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B: Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose, Kidney Int Suppl 61, 1605-16, 2002
Rat Rat, SD, 1 week Cortical collection duct Collagenase Type 2: 0.15%
 DMEM/Ham's F12 Valencia L., Bidet M., Martial S., Sanchez E., Melendez E., Tauc M., Poujeol C., Martin D., Namorado M., Reyes J., and Poujeol P.: Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture, Am J Physiol Cell Physiol 280(5), C1193, 2001
Rat Rat, SD, 250-300 g Microvessels Collagenase Type 2: 0.1%
 PSS Li, N., Teggatz, E., Li, P., Allaire, R., and Zou, A.: Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels, Microvasc Res 60, 149, 2000
Rat Rat, SD, 250-300 g Renal Collagenase Type 2: 0.2%
 see reference Mattson D., and Wu F.: Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature, Hypertension 35, 337, 2000
Rat Rat, SD, male, 5-6 week Renal tubules Collagenase: 0.1%
 see reference Miyata, N., Park, F., Li, X., and Cowley, A.: Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney, Am J Physiol 277, F437, 1999
Rat Rat, SD, 225-250 g Glomerular mesangial cells Collagenase Type 1: 250 u/ml
 DMEM Amiri F, Garcia R: Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells, Am J Physiol 276, F691-9, 1999
Rat Rat, SD, male Proximal tubule suspensions Collagenase Type 4: 0.1%
Pronase E: 2 u/ml
 HEPES buffer Eitle E, Hiranyachattada S, Wang H, Harris PJ: Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney, Am J Physiol 274, C1075-80, 1998
Rat Rat, SD, male Nephron segments Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
 Eagle's MEM Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments, Am J Physiol 273, F650, 1997
Rat Rat, 12-weeks-old Renal Collagenase: 0.1%
 DMEM Ishikawa, S., Kusaka, I., Higashiyama, M., Nagasaka, S., Saito, T., Honda, K., and Saito, T.: Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein, Nat Neurosci 50, 1506, 1996
Rat Rat, male, 150-200 g Epithelial
Proximal tubule		 Protease: 0.1%
 HBSS/CMF Elliget, K., and Trump, B.: Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury, In Vitro Cell Dev Biol 27, 739, 1991
Rat Rat, adult Inner medullary collecting duct
Papillae		 Collagenase Type 2: 0.1%
 PBS Brion, L., Schwartz, J., Lachman, H., Zavilowitz, B., and Schwartz, G.: Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells, Am J Physiol 257, F486, 1989
Rat Rat, Wistar, female, 100-150 g Glomerular mesangial Collagenase: 0.025%
 RPMI 1640 Wang, J., Kester, M., and Dunn, M.: The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells, Biochim Biophys Acta 969, 217, 1988
Rat Rat, Wistar, male, 150 - 200 g Renal target Collagenase: 1.0% (also 0.7%)
 Eagle's MEM Barlet-Bas, C., Khadouri, C., Marsey, S., and Doucet, A.: Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine, Proc Natl Acad Sci U S A 85, 1707, 1988
Rat Rat, Wistar, male, adult Proximal tubules Collagenase: 0.2%
 Krebs-Henseleit buffer Gesek, F., Wolff, D., and Strandhov, J.: Improved Separation Method for Rat Proximal and Distal Renal Tubules, Am J Physiol 253, F358, 1987
Rat Rat,Wistar, 300-400 gm Proximal tubules Collagenase: 0.15 %
 Krebs Henseleit solution Vinay, P., Gougoux, A., and Lemieux, G.: Isolation of a Pure Suspension of Rat Proximal Tubules, Am J Physiol 241, F403, 1981
Rat Rat Fibroblasts
Kidney		 Trypsin: up to 0.25%
 Dulbecco-Vogt MEM Wallach, D., Anderson, W., and Pastan, I.: Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin, J Biol Chem 253, 24, 1978