Mammary - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Bovine Bovine, lactating Holstein Mammary epithelial Collagenase Type 3: 400 u/ml
Hyaluronidase: 100 u/ml
Deoxyribonuclease I: 2 u/ml
 HBSS Miranda, S., Wang, Y., Purdie, N., Osborne, V., Coomber, B. and Cant, J.: Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture., J Dairy Sci 92, 2670, 2009
Bovine Bovine, heifer, 200kg Mammary epithelial Collagenase Type 2: 1%
Hyaluronidase: 1%
Deoxyribonuclease I: 0.03%
 M-199 Weber M., Purup S., Vestergaard M., Ellis S., Scndergard-Andersen J., Akers R., and Sejrsen K.: Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum, J Endocrinol 161, 365, 1999
Bovine Bovine Epithelial Hyaluronidase: 0.005%
 HBSS Gibson, C., Vega, J., Baumrucker, C., Oakley, C., and Welsch, C.: Establishment And Characterization Of Bovine Mammary Epithelial Cell Lines, In Vitro Cell Dev Biol 27, 585, 1991
Bovine Bovine Epithelial Deoxyribonuclease I: 0.04%
 HBSS/Medium 199 Baumrucker, C., Deemer, K., Walsh, R., Riss, T., and Akers, R.: Primary Culture of Bovine Mammary Acini on a Collagen Matrix, Tissue Cell 20 (4), 541, 1988
Bovine Bovine, young, lactating, female Mammary Collagenase: 0.30 %
 HBSS or EBSS Anderson, C., and Larson, B.: Comparative Maintenance of Function in Dispersed Cell and Organ Cultures, Exp Cell Res 61, 24, 1970
Bovine Bovine, dairy, purebred,
(also rat, Holtzmann, albino, white)		 Secretory
Mammary gland		 Collagenase: 0.02 - 0.03%
 HBSS or EBSS Schingoethe, D., Hageman, E., and Larson, B.: Essential Amino Acids for Milk Protein Synthesis in the In Vitro Secretory Cell and Stimulation by Elevated Levels, Biochim Biophys Acta 148, 469, 1967
Goat Goat, lactating, 1 month Mammary gland Collagenase: 0.02 - 0.03%
 HBSS or EBSS Blanco, A., Rife, U., and Larson, B.: Lactate Dehydrogenase Isozymes during Dedifferentiation in Cultures of Mammary Secretory Cells, Nature 214, 1331, 1967
Guinea-Pig Guinea-pig, pregnant, 4-10 day Mammary gland Trypsin NF 1:250: 0.25%
 Dulbecco phosphate Turba, F., and Hilpert, N.: Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German, Biochem Z 334, 501, 1961
Human Human Mammary epithelial Collagenase: 200 u/ml
Hyaluronidase: 100 u/ml
 DMEM/F-12 Labarge, M., Garbe, J. and Stampfer, M.: Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture., J Vis Exp 71, , 2013
Human Human, normal biopsy Fibroblasts Collagenase Type 3: 900 u/ml
 DMEM/F-12 Ronnov-Jessen L., Villadsen R., Edwards J., and Petersen O.: Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts, Am J Pathol 161, 471, 2002
Human Human Mammary epithelial cells Collagenase Type 1: 0.1%
 DMEM Huss, F. and Kratz, G.: Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue., Cells Tissues Organs 169, 361-7, 2001
Human Human Epithelial
Fibroblasts		 Collagenase Type 1: 450 IU/ml
 DMEM/Ham's F-12 Ogmundsdottir, H., Petursdottir, I., Gudmundsdottir, I., Amundadottir, L., Ronnov-Jessen, L., and Petersen, O.: Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures, In Vitro Cell Dev Biol 29A, 936, 1993
Human Human Myofibroblasts Collagenase: 900 IU/ml
 DME - F12 Ronnov-Jessen, L., VanDeurs, B., Nielsen, M., and Petersen, O.W.: Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture, In Vitro Cell Dev Biol 28, 273, 1992
Human Human, female, 15-61 yrs old Epithelial Hyaluronidase: 150 IU/ml
 DMEM Berthon, P., Pancino, G., Cremoux, P., Roseto, A., Gespach, C., and Calvo, F.: Characterization of Normal Breast Epithelial Cells in Primary Cultures: Differentiation and Growth Factor Receptors Studies, J Tiss Cul Meth 28A, 716, 1992
Human Human Epithelial Collagenase: 2.0%
 DMEM/Ham's F-12 Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples, In Vitro Cell Dev Biol 26, 1186, 1990
Human Human Tumor, breast Neuraminidase: 0.8 u/ml
 HBSS Leung, C., and Shiu, R.: Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix, In Vitro 18, 476, 1981
Human Human Epithelial Hyaluronidase: 100 u/ml
 DMEM/Ham's F-12 Stampfer, M., Hallowes, R., and Hackett, A.: Growth of Normal Human Mammary Cells in Culture, In Vitro 16 (5), 415, 1980
Mouse Mouse Mammary tumor Collagenase: 0.15%
Hyaluronidase: 0.020%
 DMEM/F12 Liu, X., Johnson, S., Liu, S., Kanojia, D., Yue, W., Singh, U., Wang, Q, Wang Qi, Nie, Q. and Chen H.: Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy., Sci Rep 3, 2473, 2013
Mouse Mouse, 10 week Fibroblasts, carcinoma Collagenase Type 4: 0.5%
Trypsin: 0.2%
Deoxyribonuclease I: 0.004
Hyaluronidase: 1,000 u/ml
 DMEM Cheng, N. and Lambert, D.: Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice., J Vis Exp 54, e2716, 2011
Mouse Mouse, 12 week Mammary epithelial Collagenase: 0.3%
Hyaluronidase: 100 u/ml
Trypsin: 0.25%
Neutral Protease: 0.5%
Deoxyribonuclease I: 0.01%
 see reference Taddei, I., Deugnier, M., Faraldo, M., Petit, V., Bouvard, D., Medina, D., Fassler, R., Thiery, J. and Glukhova, M.: Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells, Nat Cell Biol 10, 716-22, 2008
Mouse Mouse, 11 week Epithelial Collagenase Type 3: 25 u/ml
Hyaluronidase: 0.1%
Protease XIV: 0.05%
Deoxyribonuclease I: 0.04%
 DMEM/F12 Mueller, S., Clark, J., Myers, P. and Korach, K.: Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha., Endocrinology 143, 2357, 2002
Mouse Mouse Mammary epithelial stem Collagenase Type 3: 0.1%
Hyaluronidase: 0.1%
Pronase: 1.25%
Deoxyribonuclease I: 0.2%
 DMEM Boulanger CA, Smith GH: Reducing mammary cancer risk through premature stem cell senescence, Oncogene 20, 2264-72, 2001
Mouse Mouse, BALB/C Epithelium Collagenase: 250 u/ml
 HBSS Kanazawa, T., and Hosick, H.: Transformed Growth Phenotype of Mouse Mammary Epithelium in Primary Culture Induced by Specific Fetal Mesenchymes, J Cell Physiol 153, 381, 1992
Mouse Mouse, BALB/c, virgin, female, 4 month Adipocytes Trypsin: 50 µg/ml
 DMEM Beck, J., Hosick, H., and Watkins, B.: Growth of Epithelium From a Preneoplastic Mammary Outgrowth on Response to Mammary Adipose Tissue, In Vitro Cell Dev Biol 25 (5), 409, 1989
Mouse Mouse (BALB/cCrgl), mature Epithelial Pronase: 0.01%
 Hepes buffered medium 199 Bandyopadhyay, G., Imagawa, W., Wallace, D., and Nandi, S.: Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture, J Biol Chem 263, 7567, 1988
Mouse Mouse, BALB/c, female, 6-8 week Epithelial Collagenase Type 3: 0.1%
 DMEM Ehmann, U., Peterson, W., and Misfeldt, D.: To Grow Mouse Mammary Epithelial Cells in Culture, J Cell Biol 98, 1026, 1984
Mouse Mouse (C3H/HeN), female Epithelial Deoxyribonuclease I: 0.0001%
 Medium 199 Taketani, Y., and Oka, T.: EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture, Endocrinology 113, 871, 1983
Mouse Mouse, (BALB/c/Crgl Me), female, pregnant Epithelial Pepsin: 0.1% and 0.05%
Pepsin: 0.1%
 HBSS with 0.2% EDTA, CMF Riser, M., Huff, B., and Medina, D.: Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells, In Vitro 19, 730, 1983
Mouse Mouse, female Epithelial Deoxyribonuclease I: 0.1%
 DMEM Jones, W., and Hallowes, R.: Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies, J Tiss Cul Meth 8 (1), 17, 1983
Mouse Mouse (NMuMG), female, 2 months Epithelial Collagenase Type 3: 0.1%
 DMEM Ehmann, U., and Misfeldt, D.: Mouse Mammary Cells in D-Valine Medium, In Vitro 18, 407, 1982
Mouse Mouse, BALB/cCr1, female Epithelial Collagenase Type 2: 0.2%
 HBSS/DMEM Asch, B., Burstein, N., Vidrich, A., and Sun, T.: Identification of Mouse Mammary Epithelial Cells by Immunofluorescence With Rabbit and Guinea-Pig Antikeratin Antisera, Proc Natl Acad Sci U S A 78, 5643, 1981
Mouse Mouse (BALB/cfC3H) Mammary tumors
Epithelial		 Collagenase: 1.0%
 HBSS Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels, In Vitro 16, 502, 1980
Mouse Mouse, pregnant 8-12 day Mammary (see reference):
HBSS Yang J, Richards J, Guzman R, Imagawa W, and Nandi S.: Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels, Proc Natl Acad Sci U S A 77, 2088, 1980
Mouse Mouse (BALB/cCrgl), virgin, female, 4-8 month Epithelial
Mammary gland		 Collagenase Type 3: 0.1%
 HBSS White, M., Hu, A., Hamamoto, S. and Nandi: In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage, In Vitro 14, 271, 1978
Mouse Mouse (BALB/c), pregnant, 60-80 days Mammary Hyaluronidase: 0.1%
 CMF medium Ceriani, R., Peterson, J., and Abraham, S.: Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells, In Vitro 14, 887, 1978
Mouse Mouse, BALB/cfC3H/Crgl, virgin, female Nodule-transformed Hyaluronidase: 0.1%
 Medium 199 DeOme, K., Miyamoto, M., Osborn, R., Guzman, R., and Lum, K.: Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice, Cancer Res 38, 2103, 1978
Mouse Mouse, BALB/cCrgl, female
(also guinea-pig)		 Swiss 3T3 Collagenase Type 3: 0.2%
 PBS CMF Asch, B., and Medina, D.: Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells, J Natl Cancer Inst 61 (6), 1423, 1978
Mouse Mouse, (C3H/Crgl or BALB/cCrgl), 8-10 day pregnant Epithelial Collagenase Type 3: 0.12%
 HBSS Emerman, J., Enami, J., Pitelka, D., and Nandi, S.: Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes, Proc Natl Acad Sci U S A 74 (10), 4466, 1977
Mouse Mouse, lactating Parenchymal Collagenase Type 1: 0.3%
 Kreb's Ringer bicarbonate buffer Kerkof, P., and Abraham, S.: Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice, Methods Enzymol 69, 693, 1976
Mouse Mouse, female, early pregnancy <10 days Epithelial Collagenase: 0.1%
 Eagle's MEM Moore, D., and Lasfargues, E.: Method for the Continuous Cultivation of Mammary Epithelium, In Vitro 7, 21, 1971
Mouse Mouse, CBA, virgin, 9/10 wk Mammary Hyaluronidase: 0.1%
 BSS, CMF Prop, F., and Wiepjes, G.: Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse, Exp Cell Res 61, 451, 1970
Mouse Mouse, lactating, 14-18 day Parenchymal Collagenase: 0.33%
 Kreb's buffer Pitelka, D., Kerkof, P., Gagne, H., Smith, S., and Abraham, S.: Characteristics of Cells Dissociated from Mouse Mammary Glands. I. Method of Separation and Morphology of Parenchymal Cells from Lactating Glands, Exp Cell Res 57, 43, 1969
Mouse Mouse, lactating, 14 day Mammary Trypsin NF 1:250: 0.25%
 HBSS Kopelovich, L., Abraham, S., McGrath, H., DeOme, K., Chaikoff, I.: Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland, Cancer Res 26, 1534, 1966
Mouse Mouse, pregnant, 14-17 day Mammary Collagenase: 0.05% - 0.1%
 HBSS Daniel, C., and DeOme, K.: Growth of Mouse Mammary Glands In Vivo After Monolayer Culture, Science 149, 634, 1965
Mouse Mouse, adult, 1-10 days pregnant Epithelial
Mammary		 Collagenase: 0.02%
 Simm's Lasfargues, E.: Cultivation and Behavior In Vitro of the Normal Mammary Epithelium of the Adult Mouse. II. Observations on the Secretory Activity, Exp Cell Res 13, 553, 1957
Rat Rat Mammary epithelial Collagenase Type 3: 0.35%
 HBSS Mei, N., McDaniel, L., Dobrovolsky, V., Guo, X., Shaddock, J., Mittelstaedt, R., Azuma, M., Shelton, S., McGarrity, L., Doerge, D. and Heflich, R.: The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats., Toxicol Sci 115, 412, 2010
Rat Rat, female, 50 day Mammary epithelial Collagenase Type 3: 0.15%
 DMEM/F12 Maffini M., Soto A., Calabro J., Ucci A., and Sonnenschein C.: The Stroma as a Crucial Target in Rat Mammary Gland Carcinogenesis, J Cell Sci 117, 1495, 2004
Rat Rat, female Mammary gland epithelial Collagenase Type 3: 0.35%
 see reference Djuric Z., Lewis S., Lu M., Mayhugh M., Naegeli L., Tang N., and Hart R.: Effect of Varying Caloric Restriction Levels on Female Rat Growth and 5-hydroxymethyl-2'-deoxyuridine in DNA, Toxicol Sci 66, 125, 2002
Rat Rat, SD Mammary fibroblasts Collagenase Type 3: 0.2%
Neutral Protease: 0.2%
Deoxyribonuclease I: 0.01%
 DMEM/F-12 Brake P., Zhang L., and Jefcoate C.: Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids, Mol Pharmacol 54, 825, 1998
Rat Rat, SD, 50 day Mammary epithelial Collagenase Type 3: 0.2%
Neutral Protease: 0.2%
 DMEM/F12 Varela L., Darcy K., and Ip M.: The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells, Endocrinology 138, 3891, 1997
Rat Rat
(also human)		 Epithelial Collagenase Type 1: 0.4%
 DMEM Soriano, J., Pepper, M., Nakamura, T., Orci, L., and Montesano, R.: Hepatocyte Growth Factor Stimulates Extensive Development of Branching Duct-like Structures by Cloned Mammary Gland Epithelial Cells, J Cell Sci 108, 413-430, 1995
Rat Rat, SD, female, 60-90 day Epithelial Collagenase Type 3: 0.5%
 EBSS Laduca, J., and Sinha, D.: In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso-N-Methylurea Using a Collagen Gel Matrix Culture, In Vitro Cell Dev Biol 29A, 789, 1993
Rat Rat, Lewis, female, 90 days Epithelial
Mammary		 Collagenase: 0.05%
 Medium 199 Lin, T., Hom, Y.K., Richards, J. and Nandi, S.: Effects of Antioxidants and Reduced Oxygen Tension on Rat Mammary Epithelial Cells in Culture, In Vitro Cell Dev Biol 27A, 191, 1991
Rat Rat, SD, female, 55 day Epithelial Neutral Protease: 3 u/ml
 Medium 199 Ehmann, U., Osborn, R., Guzman, R., and Fajardo, L.: Cultured Proliferating Rat Mammary Epithelial Cells, In Vitro Cell Dev Biol 27, 749, 1991
Rat Rat, 50-60 days Epithelial Neutral Protease: 0.2%
 EBSS Hahm, H.A., Ip, M.M.: Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors, In Vitro Cell Dev Biol 26, 791, 1990
Rat Rat, Fisher 344, virgin, 80-100 day Epithelial Hyaluronidase: 0.1%
 Medium 199 McGrath, M., Palmer, S., and Nandi, S.: Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF, J Cell Physiol 125, 182, 1985
Rat Rat, LEW, virgin, female, 45-50 day Epithelial Collagenase: 0.1%
 Medium 199 Ethler, S.: Primary Culture and Serial Passage of Normal and Carcinogen-Treated Rat Mammary Epithelial Cells In Vitro, J Natl Cancer Inst 74 (6), 1307, 1985
Rat Rat, SD, female Epithelial, cancer and tumor Collagenase: 0.1%
 Eagles's MEM Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma, In Vitro 18, 565, 1982
Rat Rat, inbred LEW, female, 50-60 day old Epithelial Collagenase Type 3: 0.1%
 Medium 199 Richards, J., and Nandi, S.: Primary Culture of Rat Mammary Epithelial Cells. I. Effect of Plating Density, Horomones, and Serum on DNA Synthesis, J Natl Cancer Inst 61 (3), 765, 1978
Rat Rat, Wistar, 13-18 day postpartum Acini Collagenase: 0.05%
 HBSS Katz, J., Wals, P. and Van de Velde, R: Lipogenesis by Acini from Mammary Gland of Lactating Rats, J Biol Chem 249, 7348, 1974
Rat Rat, SD, 15-20 day postpartum Mammary Collagenase: 0.2%
 Kreb's Ringer bicarbonate buffer Martin, R., and Baldwin, R.: Effects of Insulin on Isolated Rat Mammary Cell Metabolism: Glucose Utilization and Metabolite Patterns, Endocrinology 89, 1263, 1971
Rat Rat, virgin Mammary Collagenase: 0.35%
 Medium 199 Moon, R., Janns, D., and Young, S.: Preparation of Fat Cell-"Free" Rat Mammary Gland, J Histochem Cytochem 17 (3), 182, 1969