Neural - Tissue Tables

Species	Species Detail	Cells	Enzyme(s)	Medium	Reference
Avian	Quail and Chicken, embryonic	Midgut DRG	Collagenase animal free: 0.05% Neutral Protease: 0.5%	Ham's F12	Rollo, B., Zhang, D., Simkin, J., Menheniott, T. and Newgreen, D.: Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells., F1000Res 4, 113, 2015
Avian	Chick, White Leghorn, embryos	Cilary ganglion neurons	Collagenase Type 1: 0.1%	PBS	Temburni, M., Rosenberg, M., Pathak, N., McConnell, R. and Jacob, M.: Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein., J Neurosci 24, 6776, 2004
Bovine	Bovine (also rat)	Heart Adrenal chromaffin Paraneurons	Trypsin: 0.06%	25mM HEPES buffered Locke's solution, CMF	Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Bovine	Bovine	Microvascular endothelial	Collagenase/Dispase: 0.1%	MEM	Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina, In Vitro 18 (7), 626, 1982
Bovine	Calf (also lamb)	Oligodendroglia Neural	Trypsin: 0.1%	(see reference)	Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253, 1592, 1978
Canine	Canine	Satellite glial	Hyaluronidase: 0.2% Collagenase Type 4: 0.2% Collagenase: 0.2%	HBSS	Tongtako, W., Lehmbecker, A., Wang, Y., Hahn, K., Baumgartner, W. and Gerhauser, I.: Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties., Sci Rep 7, 13915, 2017
Chicken	Chick, White Leghorn, embryos	Islets	Trypsin: Trypsin: 0.125-0.25% Collagenase: 0.2% Deoxyribonuclease I: 200 u/ml	Eurocollins solution	Watt, P., Mullen, Y., Benhamou, P., Hober, C., Nomura, Y., Watanabe, Y., Passaro, E., Zinner, M., and Brunicardi, F.: Simplified Semiautomated Method For Isolating Islets From the Human Pancreas, Transplant Proc 26 (2), 582, 1994
Chicken	Chicken, White Leghorn, embryos, 17-21 day	Neurons	Papain: 40 u/ml	HEPES	Raman, I., and Trussell, L.: The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus, Neuron 9, 173, 1992
Chicken	Chick, embryo, 10-14 day	Flat, retina	Trypsin: 0.1%	Tyrode's solution, CMF	Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina, In Vitro Cell Dev Biol 26, 1073, 1990
Chicken	Chick, White Leghorn, embryo (also rat, SD, embryo)	Spinal cord	Trypsin: 0.05%	Phosphate buffer (see reference)	Schnaar, R., and Schaffner, A.: Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions, J Neurosci 1, 204, 1981
Chicken	Chick	Dorsal root ganglion neurons Spinal cord	Trypsin: 0.1%	Puck's saline, CMF	Choi, D., and Fiscbach, G.: GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture, J Neurophysiol 45, 605, 1981
Chicken	Chick, White Leghorn embryo, 8 day	Neurons, ganglia	Trypsin: 0.25%	HBSS, CMF	Bottenstein, J., Skaper, S., Varon, S., and Sato, G.: Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium, Exp Cell Res 125, 183, 1980
Chicken	Chicken, White Leghorn, fertile eggs	Ciliary ganglion neurons	Trypsin: 0.25%	Eagle's MEM	Tuttle, J., Suszkiw, J., and Ard, M.: Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture, Brain Res 183, 161, 1980
Chicken	Chicken, embryos, 9-10 days old	Dorsal root ganglia neurons	Collagenase: 0.01%	Eagle's MEM	Mudge, A., Leeman, S., and Fischbach, G.: Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration, Proc Natl Acad Sci U S A 76 (1), 526, 1979
Chicken	Chick embryos	Ganglion chains, sympathetic ganglia	Trypsin: 0.25%	Krebs Phosphosaline	McCarthy, K., and Partlow, L.: Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates, Brain Res 114, 391, 1976
Chicken	Chick, White Leghorn, embryos, 7 day	Neural retina	Collagenase: 0.25% Elastase: 0.2% Hyaluronidase: 1.0% Papain: 1.0% Protease: 0.1% Trypsin: 0.05%	HBSS	Wiseman, L., and Hammond, W.: The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents, J Exp Zool 197, 429, 1976
Fish	Zebrafish, embryonic	Motorneurons	PDS kit: per instructions	L-15	Spiro, Z., Koh, A., Tay, S., See, K. and Winkler, C.: Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish., Sci Rep 6, 27470, 2016
Fish	Zebrafish	Motor neurons	Collagenase Type 2: 0.1%	See reference	Sakowski, S., Lunn, J., Busta, A., Palmer, M., Dowling, J. and Feldman, E.: A Novel Approach to Study Motor Neurons from Zebrafish Embryos and Larvae in Culture., J Neurosci Methods 205, 277-82, 2012
Fish	Zebrafish, Dania rerio, embryo	Rohon-Beard neurons	Trypsin: 0.2%	L-15/Hepes	Won, Y., Ono, F. and Ikeda, S.: Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons., J Neurophysiol 105, 442-53, 2011
Fish	Zebrafish, Danio rerio, embryo	Neurons	PDS kit: with modifications	EBSS	Cerda, G., Hargrave, M. and Lewis, K.: RNA Profiling of FAC-sorted Neurons from the Developing Zebrafish Spinal Cord., Dev Dyn 238, 150-61, 2009
Fish	Black ghose knige fish, adult (Apteronotus albifrons)	Neurons, spinal cord	Trypsin: 0.4%	PBS, CMF	Anderson, M.J.: Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro, In Vitro Cell Dev Biol 29A, 145, 1993
Frog	Xenopus, embryonic	Neuron	Collagenase Type 1: 0.1%	Steinberg's solution	Takahashi, T., Nakajima, Y., Hirosawa, K., Nakajima, S., and Onodera, K.: Structure and Physiology of Developing Neuromuscular Synapses in Culture, J Neurosci 7, 473, 1987
Guinea-Pig	Guinea-pig, newborn	Neuron, enteric	Trypsin: 0.125%	Medium 199	Jessen, K, McConnell, J., Purves, R., Burnstock, G., and Chamley-Campbell, J.: Tissue Culture of Mammalian Enteric Neurons, Brain Res 152, 573, 1978
Hamster	Hamster, male	Vomeronasal organ neurons	Collagenase Type 1: 0.02% Trypsin: 0.02%	PBS	Liman ER: Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons, J Physiol 548, 777, 2003
Human	Human	Spinal cord neural progenitor	PDS kit: with modifications	EBSS	Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015
Human	Human	Neural, various	Papain: 12 u/ml Trypsin: see reference Collagenase/Dispase: see reference	see reference	Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24, Stem Cells 25, 1560-70, 2007
Human	Human, 9 month	Neurons	Collagenase Type 4: 1.33% Papain: 0.07 u/ml Neutral Protease: 1 mg/ml	DMEM/F12	Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.: EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy, Nat Med 11, 277, 2005
Human	Human, 26 week	Neural progenitor cells	Papain: 2.5 u/ml Deoxyribonuclease I: 250 u/ml Neutral Protease: 1 u/ml	DMEM/F-12	Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.: Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells, J Neurosci Res 75, 782, 2004
Human	Human, adult	Ventricular epithelial	Papain: 11.4 u/ml Deoxyribonuclease I: 10 u/ml	DMEM/F12	Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA: Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone, J Neurosci Res 59, 321, 2000
Human	Human, 5-65 years	Retinal pigment epithelial (RPE)	Trypsin: 0.25%	HBSS	Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture, Exp Eye Res 69, 97, 1999
Human	Human	Dorsal root ganglion neurons	Collagenase Type 1: 0.2% Neutral Protease: 0.5%	DMEM/F-12	Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.: Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons, F.E.B.S. Lett. 462, 117, 1999
Insect	Drosophila, larval	Neuroblasts	Collagenase Type 1: 0.1% Papain: 0.1%	Rinaldini solution	Berger, C., Harzer, H., Burkard, T., Steinmann, J., van der Horst, S., Laurenson, A., Novatchkova, M., Reichert, H. and Knoblich, J.: FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal., Cell Rep 2, 407, 2012
Insect	Drosophila	Dendrites	Collagenase: 0.05% Neutral Protease: 0.2%	HBSS	Sanchez-Soriano, N., Bottenberg, W., Fiala, A., Haessler, U., Kerassoviti, A., Knust, E., Lohr, R. and Prokop, A.: Are Dendrites in Drosophila Homologous to Vertebrate Dendrites?, Dev Biol 288, 126, 2005
Insect	Gryllus Bimaculatus	Giant interneurons	Collagenase: 0.05% Neutral Protease: 0.2%	Leibovitz's L15	Kloppenburg, P. and Horner, M.: Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus, J Exp Biol 201 (Pt 17), 2529, 1998
Monkey	Macaque	Dorsal root ganglia	Papain: 35 u/ml Deoxyribonuclease I: 80 u/ml Stemxyme 1: 0.2%	see reference	Kupari, J., Usoskin, D., Parisien, M., Lou, D., Hu, Y., Fatt, M., Lonnerberg, P., Spangberg, M., Eriksson, B., Barkas, N., Kharchenko, P., Lore, K, Khoury, S., Diatchenko, L. and Ernfors, P.: Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain., Nat Commun 12, 1510, 2021
Mouse	Mouse	Dorsal root ganglion	Stemxyme 1: 0.1-0.2%	HBSS	Yousuf, M., Samtleben, S., Lamothe, S., Friedman, T., Catuneanu, A., et al.: ER Stress in the Dorsal Root Ganglia Regulates BK Channel Physiology and Contributes to Pain Hypersensitivity in a Mouse Model of Multiple Schlerosis, bioRxivorg , , 2020
Mouse	Mouse, embryonic 12.5	Schwann cell precursors	Collagenase Type 2: 0.2% Hyaluronidase: 0.12% Soybean Trypsin Inhibitor: 0.03%	DMEM/ Ham's F-12	Mirsky, R. and Jessen, K.: Isolation of Schwann Cell Precursors from Rodents., Methods Mol Biol 1739, 3-15, 2018
Mouse	Mouse, embryonic	Cortical neurons	PDS kit: per instructions	Neurobasal	Kramer, N., Haney, M., Morgens, D., Jovicic, A., Couthouis, J., Li, A., Ousey, J., Ma, R., Bieri, G., Tsui, C., Shi, Y., Hertz, N., Tessier-Lavigne, M., Ichida, J., Bassik, M. and Gitler, A.: CRISPR-Cas9 Screens in Human Cells and Primary Neurons Identify Modifiers of C9ORF72 Dipeptide-Repeat-Protein Toxicity., Nat Genet , , 2018
Mouse	Mouse, P2-P12	Dorsal root ganglia neurons	Collagenase Type 2: 0.1%	MEM	Fangmann, L., Teller, S., Stupakov, P., Friess, H., Ceyhan, G. and Demir, I.: 3D Cancer Migration Assay with Schwann Cells., Methods Mol Biol 1739, 317-325, 2018
Mouse	Mouse, adult	Lumbar DRG	Collagenase Type 2: 200 u/ml Neutral Protease: 2.5 u/ml	HBSS	Weng, Y., An, R., Cassin, J., Joseph, J., Mi, R., Wang, C., Zhong, C., Jin, S., Pfeifer, G., Bellacosa, A., Dong, X., Hoke, A., He, Z., Song, H. and Ming, G.: An Intrinsic Epigenetic Barrier for Functional Axon Regeneration, Neuron 94, 337, 2018
Mouse	Mouse	Spiral ganglion	Collagenase Type 1: 0.1%	MEM	Meas, S., Nishimura, K., Scheibinger, M. and Dabdoub, A.: Front Neurosci 12, 822, 2018
Mouse	Mouse, 4 month	Satellite glial	Hyaluronidase: 0.2% Collagenase Type 4: 0.2% Collagenase: 0.2%	HBSS	Tongtako, W., Lehmbecker, A., Wang, Y., Hahn, K., Baumgartner, W. and Gerhauser, I.: Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties., Sci Rep 7, 13915, 2017
Mouse	Mouse	Neurospheres	Trypsin: 0.13% Hyaluronidase: 0.08%	Neurobasal A	Xu, W., Sachewsky, N., Azimi, A., Hung, M., Gappasov, A. and Morshead, C.: Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord., Stem Cells 35, 485-496, 2017
Mouse	Mouse, day 2	Schwann	Trypsin: 0.025% Collagenase Type 1: 0.1% Deoxyribonuclease I: 7 u/ml Collagenase Type 2: 0.2%	DMEM	Clements, M., Byrne, E., Camarillo Guerrero, L., Cattin, A., Zakka, L., Ashraf, A., Burden, J., Khadayate, S., Lloyd, A., Marguerat, S. and Parrinello, S.: The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration., Neuron 96, 98-114.e7, 2017
Mouse	Mouse, 2-3 month	Sympathetic neurons	Papain: 10 u/ml Collagenase Type 2: 0.13% Neutral Protease: 0.22%	EBSS	Sun, H., Tsai, W., Li, B., Tao, W., Chen, P. and Rubart, M.: Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice., PLoS ONE 11, e0148962, 2016
Mouse	Mouse	Spinal motor neurons, astrocytes, microglia	Papain: 0.2%	HBSS	Beaudet, M., Yang, Q., Cadau, S., Blais, M., Bellenfant, S., Gros-Louis, F. and Berthod, F.: High Yield Extraction of Pure Spinal Motor Neurons, Astrocytes and Microglia from Single Embryo and Adult Mouse Spinal Cord., Sci Rep 5, 16763, 2015
Mouse	Mouse	Neurons	PDS kit: per instructions	EBSS	Ren, H., Lu, T., McGraw, T. and Accili, D.: Anorexia and Impaired Glucose Metabolism in Mice with Hypothalamic Ablation of Glut4 Neurons., Diabetes 64, 405-17, 2015
Mouse	Mouse, adult	Astrocytes	Papain: ~20 u/ml	HBSS	Choi, H., Sun, D. and Jakobs, T.: Isolation of Intact Astrocytes from the Optic Nerve Head of Adult Mice., Exp Eye Res 137, 103-10, 2015
Mouse	Mouse	DRG neurons	Collagenase Type 2: 0.1% Trypsin: 0.25%	DMEM/F-12	Lee, B., Cho, H., Jung, J., Yang, Y., Yang, D. and Oh, U.: Anoctamin 1 Contributes to Inflammatory and Nerve-Injury Induced Hypersensitivity., Mol Pain 10, 5, 2014
Mouse	Mouse, neonatal	Schwann cells	Collagenase: 0.05-0.1% Trypsin: 0.125-0.25%	DMEM	Stettner, M., Lohmann, B., Wolffram, K., Weinberger, J., Dehmel, T., Hartung, H., Mausberg, A. and Kieseier, B.: Interleukin-17 Impedes Schwann Cell-Mediated Myelination., J Neuroinflammation 11, 63, 2014
Mouse	Mouse	Oligodendrocytes	Papain: 0.1% Deoxyribonuclease I: 0.0002%	HBSS	Harris, M., Hulseberg, P., Ling, C., Karman, J., Clarkson, B., Harding, J., Zhang, M., Sandor, A., Christensen, K., Nagy, A., Sandor, Matyas,S. and Fabry, Z.: Immune Privilege of the CNS is not the Consequence of Limited Antigen Sampling., Sci Rep 4, 4422, 2014
Mouse	Mouse	Hippocampal neurons	PDS kit: per instructions	DMEM	Williams, E., Zhong, X., Mohamed, A., Li, R., Liu, Y., Dong, Q., Ananiev, G., Mok, J., Lin, B., Lu, J., Chiao, C., Cherney, R., Li, H., Zhang, S. and Chang, Q: Mutant Astrocytes Differentiated from Rett Syndrome Patients-Specific iPSCs Have Adverse Effects on Wild-Type Neurons., Hum Mol Genet 23, 2968-80, 2014
Mouse	Mouse	Neurosperes	Papain: 0.1% Deoxyribonuclease I: 0.1%	NeuroCult NSC	Stuart, M., Corrigan, F. and Baune, B.: Knockout of CXCR5 Increases the Population of Immature Neural Cells and Decreases Proliferation in the Hippocampal Dentate Gyrus., J Neuroinflammation 11, 31, 2014
Mouse	Mouse, 2 month	Fibroblasts	Papain: 0.2%	Hibernate	Liu, G., Rustom, N., Litteljohn, D., Bobyn, J., Rudyk, C., Anisman, H. and Hayley, S.: Use of Induced Pluripotent Stem Cell Derived Neurons Engineered to Express BDNF for Modulation of Stressor Related Pathology., Front Cell Neurosci 8, 316, 2014
Mouse	Mouse, 4-6 week	Schwann	Collagenase Type 1: 0.01% Trypsin: 0.125%	DMEM	Masaki, T., Qu, J., Cholewa-Waclaw, J., Burr, K., Raaum, R. and Rambukkana, A.: Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection., Cell 152, 51, 2013
Mouse	Mouse	Oligodendrocyte precursor cells	Papain: 0.09%	DMEM	Medina-Rodriguez, E., Arenzana, F., Bribian, A. and de Castro, F.: Protocol to Isolate a Large Amount of Functional Oligodendrocyte Precursor Cells from the Cerebral Cortex of Adult Mice and Humans., PLoS ONE 8, e81620, 2013
Mouse	Mouse	Enteric neurons	Collagenase Type 4: 0.1% Trypsin: 0.05% Deoxyribonuclease I: 400 u/ml	DMEM/F12	Zhang, Y. and Hu, W.: Mouse Enteric Neuronal Cell Culture., Methods Mol Biol 1078, 55-63, 2013
Mouse	Mouse, 6-8 week	Neural precursors	Trypsin: 0.13% Hyaluronidase: 0.08%	see reference	Babona-Pilipos, R., Popovic, M. and Morshead, C.: A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field., J Vis Exp 68, e4193, 2012
Mouse	Mouse, adult	Retinal neurons	Papain: 10-20 ul	HBSS	Goetz, J. and Trimarchi, J.: Single-Cell Profiling of Developing and Mature Retinal Neurons., J Vis Exp 62, e3824, 2012
Mouse	Mouse	Neurons	Papain: 100 ul	DMEM	Li, Y., Roy, B., Wang, W., Zhang, L., Sampson, S. and Lin, D.: Imaging pHluorin-Tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons., J Vis Exp 69, e4450, 2012
Mouse	Mouse, P4-5	Neuroshere	Trypsin: 0.25% Papain: 100 u Deoxyribonuclease I: 0.025%	Pro-N	Ziegler, A., Schneider, J., Qin, M., Tyler, W., Pintar, J., Fraidenraich, D., Wood, T and Levison, S.: IGF-II Promotes Stemness of Neural Restricted Precursors., Stem Cells 30, 1265, 2012
Mouse	Mouse, 6-8 week	Neural stem cells	Papain: 0.01% Neutral Protease: 0.1% Deoxyribonuclease I: 0.01%	DMEM/F12	Bracko, O., Singer, T., Aigner, S., Knobloch, M., Winner, B., Ray, J., Clemenson, G., Suh, H., Couillard-Despres, S., Aigner, L., Gage, F. and Jessberger, S.: Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis., J Neurosci 32, 3376-87, 2012
Mouse	Mouse	Nerve progenitors	Collagenase Type 4: 0.025% Trypsin NF 1:250: .025%	HBSS	Salisbury, E., Lazard, Z., Ubogu, E., Davis, A. and Olmsted-Davis, E.: Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2., Stem Cells Transl Med 1, 874-85, 2012
Mouse	Mouse, embryonic	Neural crest	Collagenase/Dispase: 0.1%	DMEM	Pfaltzgraff, E., Mundell, N. and Labosky, P.: Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube., J Vis Exp , e4134, 2012
Mouse	Mouse, embryo, 14 day	Spinal cord neurons	Papain: 0.05% Deoxyribonuclease I: 0.004%	PBS/DMEM	Pollari, E., Savchenko, E., Jaronen, M., Kanninen, K., Malm, T., Wojciechowski, S., Ahtoniemi, T., Goldsteins, G., Giniatullina, R., Giniatullin, R., Koistinaho, J. and Magga, J.: Granulocyte Colony Stimulating Factor Attenuates Inflammation in a Mouse Model of Amyotrophic Lateral Sclerosis., J Neuroinflammation 8, 74, 2011
Mouse	Mouse, 1-2 day	Oligodendrocytes, dorsal root ganglia	Papain: 0.15% Deoxyribonuclease I: 0.006%	DMEM	O'Meara, R., Ryan, S., Colognato, H. and Kothary, R.: Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues., J Vis Exp 54, 3324, 2011
Mouse	Mouse, embryonic	Motorneurons	Trypsin: 0.025%	HBSS	Conrad, R., Jablonka, S., Sczepan, T., Sendtner, M., Wiese, S. and Klausmeyer, A.: Lectin-Based Isolation and Culture of Mouse Embryonic Motoneurons., J Vis Exp 55, e3200, 2011
Mouse	Mouse, 4-8 week	Olfactory ensheathing	Collagenase: 0.15% Papain: 12 u/ml	DMEM	Radtke, C., Sasaki, M., Lankford, K., Gallo, V. and Kocsis, J.: CNPase Expression in Olfactory Ensheathing Cells., J Biomed Biotechnol 2011, 608496, 2011
Mouse	Mouse	Dentate gyrus neural precursor	Papain: 2.5 u/ml Neutral Protease: 1.0 u/ml Deoxyribonuclease I: 250 u/ml	Neurobasal A	Babu, H., Claasen, J., Kannan, S., Runker, A., Palmer, T. and Kempermann, G.: A Protocol for Isolation and Enriched Monolayer Cultivation of Neural Precursor Cells from Mouse Dentate Gyrus., Front Neurosci 5, 89, 2011
Mouse	Mouse, 30 day	Motoneurons	Papain: 0.2%	Hibernate A	Milligan, C. and Gifondorwa, D.: Isolation and Culture of Postnatal Spinal Motoneurons., Methods Mol Biol 793, 77, 2011
Mouse	Mouse	Hippocampal and retinal neurons	Papain: 1% Deoxyribonuclease I: 5 u/ml	HBSS	Brown, J., Gianino, S. and Gutmann, D.: Defective cAMP Generation Underlies the Sensitivity of CNS Neurons to Neurofibromatosis-1 Heterozygosity, J Neurosci 30, 5579, 2010
Mouse	Mouse	CNS leukocytes	Collagenase Type 4: 300 u/ml	HBSS	Sayed, B., Christy, A., Walker, M. and Brown, M.: Meningeal Mast Cells Affect Early T Cell Central Nervous System Infiltration and Blood-Brain Barrier Integrity Through TNF: a Role for Neutrophil Recruitment?, J Immunol 184, 6891-900, 2010
Mouse	Mouse, adult	Spinal microganglia	Papain: 0.2%	Hibernate A	Yip, P., Kaan, T., Fenesan, D. and Malcangio, M.: Rapid Isolation and Culture of Primary Microglia from Adult Mouse Spinal Cord., J Neurosci Methods 183, 223-37, 2009
Mouse	Mouse, embryonic	Dopaminergic neurons	Trypsin: 0.1% Deoxyribonuclease I: 0.02%	DMEM	Radad Khaled, Gille Gabriele, Rausch Wolf-Dieter: Dopaminergic neurons are preferentially sensitive to long-term rotenone toxicity in primary cell culture, Toxicol In Vitro 22, 68-74, 2008
Mouse	Mouse, neonatal	DRG neurons	Collagenase: 0.2% Trypsin: 0.05%	Ham's F12	Pedrola, L., Espert, A., Valdes-Sanchez, T., Sanchez-Piris, M., Sirkowski, E., Scherer, S., Farinas, I., and Palau, F.: Cell Expression of GDAP1 in the Nervous System and Pathogenesis of Charcot-Marie-Tooth Type 4A Disease., J Cell Mol Med 12, 679, 2008
Mouse	Mouse, 6 mo	Neurons, neurospheres	Papain: 0.2%	Hibernate	Brewer Gregory J, Torricelli John R: Isolation and culture of adult neurons and neurospheres, Nat Protoc 2, 1490-8, 2007
Mouse	Mouse	Neural, various	Papain: 12 u/ml Trypsin: see reference Collagenase/Dispase: see reference	see reference	Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24, Stem Cells 25, 1560-70, 2007
Mouse	Mouse, 6-8 week	Sensory neurons, DRG	Papain: 20 u/ml Collagenase Type 2: 0.4% Neutral Protease: 0.46%	HBSS	Malin, S., Davis, B. and Molliver, D.: Production of Dissociated Sensory Neuron Cultures and Considerations for their use in Studying Neuronal Function and Plasticity, Nat Protoc 2, 152, 2007
Mouse	Mouse, C57BL/6, 1 year	Neurons	Papain: 0.2%	DMEM	Eide, L, and McMurray, C: Culture of Adult Mouse Neurons, Biotechniques 38(1), 99-104, 2005
Mouse	Mouse, adult	Brain and spinal cord cells	Trypsin: 0.25%	PBS	Gonzalez John M, Bergmann Cornelia C, Fuss Babette, Hinton David R, Kangas Cindy, Macklin Wendy B, Stohlman Stephen A: Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes, Glia 51, 22-34, 2005
Mouse	Mouse, neonatal	Neurons	PDS kit: per instructions	EBSS	Gill JC, Moenter SM, Tsai PS: Developmental regulation of gonadotropin-releasing hormone neurons by fibroblast growth factor signaling, Endocrinology 145, 3830, 2004
Mouse	Mouse, C57BL	Cerebellar granule cell precursors	Papain: 16.5 u/ml Deoxyribonuclease I: 0.008%	Dulbecco's PBS	Okano-Uchida T, Himi T, Komiya Y, and Ishizaki Y: Cerebellar granule cell precursors can differentiate into astroglial cells, Proc Natl Acad Sci U S A 101, 1211, 2004
Mouse	Mouse, 1-3 day	Neurons, ganglia	Papain: 20 u/ml Deoxyribonuclease I: 100 u/ml Collagenase: 0.3% Trypsin: 0.05%	HBSS	Savchenko V, Sung U, Blakely RD.: Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody, Mol Cell Neurosci 24, 1131, 2003
Mouse	Mouse, male	Trigeminal senesory neurons	Papain: 20 u/ml	HEPES buffered saline	Roberts, L., MacDonald, C.and Mark, C.: Anandamide is a Partial Agonist at Native Vanilloid Receptors in Acutely Isolated Mouse Trigeminal Sensory Neurons., Br J Pharmacol 137, 421, 2002
Mouse	Mouse, postnatal-day-1-old (P1)	Neurons	Trypsin: 0.25%	NGF-containing medium	Deshmukh, M., Kuida, K., and Johnson Jr., E.: Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization, J Cell Biol 150 (1), 131, 2000
Mouse	Mouse embryos	Neurons, DRG and SCG	Trypsin: 0.25%	L-15 medium	Lee, K., Davies, A., and Jaenisch, R.: P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF, Development 120, 1027, 1994
Mouse	mouse, 65 days	Neurons, neuronal precursors	Trypsin: 0.1% Deoxyribonuclease I: 0.001%	DMEM	Richards L., Kilpatrick T., and Bartlett P.: De Novo Generation of Neuronal Cells from the Adult Mouse Brain, Proc Natl Acad Sci U S A 89, 8591, 1992
Mouse	Mouse, CD-1, neonate	Neurons, dorsal root ganglion	Trypsin: 0.25%	HBSS	Quinn, S. and De Boni, U.: Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro, In Vitro Cell Dev Biol 27, 55, 1991
Mouse	Mouse, fetal	Precursor	Trypsin: 0.5%	PBS	Kitani, H., Shiurba, R., Sakakura, T., Tomooka, Y.: Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture, In Vitro Cell Dev Biol 27, 615, 1991
Mouse	Mouse (SWR or CF1), 1-3 months	Papillae, taste receptor	Pronase E: 0.15%	Carbonate-Phosphate buffer (see reference)	Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.: A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells, Brain Res 503, 326, 1989
Mouse	Mouse, neonatal (also chick)	PNS test neurons	Trypsin: 0.08%	Eagle's Basal Medium (see reference)	Varon, S., Skaper, S., Barbin, G., Selak, I., and Manthorpe, M.: Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System, J Neurosci 4 (3), 654, 1984
Mouse	Mouse (BALB/c), adult	Neurons, spinal cord	Collagenase Type 3: 0.25%	Hank's BSS, CMF	Eagleson, K. and Bennett, M.: Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium, Neurosci Lett 38, 187, 1983
Mouse	Mouse, 0-30 day	Neural	Trypsin NF 1:250: 0.25%	BSS	Shrier, B., Wilson, S., and Nirenberg, M.: Cultured Cell Systems and Methods for Neurobiology, Vol. 32,, , 765, 1974
Ovine	Lamb (also calf)	Oligodendroglia Neural	Trypsin: 0.1%	(see reference)	Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253, 1592, 1978
Porcine	Porcine, adult, 60-100 kg	Superior cervical ganglia	Papain: 2 u/ml Collagenase: 0.12% Neutral Protease: 0.48%	HBSS	Si ML, Lee TJ.: Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries, J Pharmacol Exp Ther 298, 122, 2001
Quail	Quail	Neural crest	Trypsin: 0.05%	MEM, HBSS	Sieber-Blum, M., and Cohen, A.: Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells, Dev Biol 80, 96, 1980
Rat	Rat, embryonic 14.5 day	Schwann cell precursors	Collagenase Type 2: 0.2% Hyaluronidase: 0.12% Soybean Trypsin Inhibitor: 0.03%	DMEM/ Ham's F-12	Mirsky, R. and Jessen, K.: Isolation of Schwann Cell Precursors from Rodents., Methods Mol Biol 1739, 3-15, 2018
Rat	Rat, SD, 3 month	Schwann	Neutral Protease: 0.25% Collagenase Type 1: 0.05%	DMEM	Andersen, N. and Monje, P.: Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers., Methods Mol Biol 1739, 49-66, 2018
Rat	Rat, Wistar, 3 day	Dorsal root ganglia neurons	Collagenase Type 2: 0.1%	MEM	Fangmann, L., Teller, S., Stupakov, P., Friess, H., Ceyhan, G. and Demir, I.: 3D Cancer Migration Assay with Schwann Cells., Methods Mol Biol 1739, 317-325, 2018
Rat	Rat, SD, day 2	Schwann	Collagenase Type 1: 0.1% Trypsin: 0.25%	DMEM	Maurel, P.: Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies., Methods Mol Biol 1739, 17-37, 2018
Rat	Rat, adult	Schwann	Stemxyme 1: 0.05%	DMEM	George, D., Ahrens, P. and Lambert, S.: Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells., Glia , , 2018
Rat	Rat, 2-3 week	Dorsal root ganglia	Collagenase Type 1: 0.1%	HBSS	Lin, Y. and Chen, J.: Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release., J Vis Exp , , 2018
Rat	Rat, SD, p7	Schwann	Trypsin: 0.025% Collagenase Type 1: 0.1% Deoxyribonuclease I: 7 u/ml Collagenase Type 2: 0.2%	DMEM	Clements, M., Byrne, E., Camarillo Guerrero, L., Cattin, A., Zakka, L., Ashraf, A., Burden, J., Khadayate, S., Lloyd, A., Marguerat, S. and Parrinello, S.: The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration., Neuron 96, 98-114.e7, 2017
Rat	Rat, SD	Schwann	Collagenase Type 1: 0.05%	DMEM	Schuh, C., Hercher, D., Stainer, M., Hopf, R., Teuschl, A., Schmidhammer, R. and Redl, H.: Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture., Cytotherapy 18, 760-70, 2016
Rat	Rat, E17	Neurons	PDS kit: per instructions	DMEM	Hayakawa, K., Esposito, E., Wang, X., Terasaki, Y., Liu, Y., Xing, C., Ji. X. and Lo, E.: Transfer of Mitochondria from Astrocytes to Neurons after Stroke., Nature 535, 551-5, 2016
Rat	Rat, SD	Schwann	Neutral Protease: 0.25% Collagenase Type 1: 0.05% Trypsin: 0.25%	DMEM	Andersen, N., Srinivas, S., Pinero, G. and Monje, P.: A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves., Sci Rep 6, 31781, 2016
Rat	Rat, embryonic and neonatal	Cortical neurons, DRG	PDS kit: per instructions Collagenase Type 1: 0.17%	Neurobasal	Rheaume, C., Cai, B., Wang, J., Fernandez-Salas, E., Aoki, K., Francis, J. and Broide, R.: A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25., Toxins 7, 2354-70, 2015
Rat	Rat, SD, 6-9 week	Dorsal root ganglia	Collagenase Type 3: 0.2%	HBSS	Murayama, C., Watanabe, S., Nakamura, M. and, Norimoto, H.: Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons., Molecules 20, 14959-69, 2015
Rat	Rat	Dorsal root ganglion neurons	Collagenase Type 2: 0.15%	HBSS	Yu, R., Seymour, V., Berecki, G., Jia, X., Akca, M., Adams, D., Kaas, Q. and Craik, D.: Less is More: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic α-Conotoxin Vc1.1., Sci Rep 5, 13264, 2015
Rat	Rat, 6-8 week	Spinal cord neural progenitor	PDS kit: with modifications	EBSS	Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015
Rat	Rat, SD	Trigeminal ganglion	Collagenase Type 2: 0.1% Papain: 20 u/ml	DMEM/F12	Wei, X., Yan, J., Tillu, D., Asiedu, M., Weinstein, N., Melemedjian, O., Price, T. and Dussor, G.: Meningeal Norepinephrine Produces Headache Behaviors in Rats via Actions Both on Dural Afferents and Fibroblasts., Cephalalgia 35, 1054-64, 2015
Rat	Rat, embryonic	Cerebral neurons	Papain: 2 u/ml Deoxyribonuclease I: 0.01%	DMEM-PBS	Oyanagi, K., Tashiro, T. and Negishi, T.: Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes., J Toxicol Sci 40, 459-68, 2015
Rat	Rat, neonatal	Schwann cells	Collagenase: 0.05-0.1% Trypsin: 0.125-0.25%	DMEM	Stettner, M., Lohmann, B., Wolffram, K., Weinberger, J., Dehmel, T., Hartung, H., Mausberg, A. and Kieseier, B.: Interleukin-17 Impedes Schwann Cell-Mediated Myelination., J Neuroinflammation 11, 63, 2014
Rat	Rat, 1-3 day	Hippocampal neurons	Papain: 25 u/ml	L-15	Thurner, P., Gsandtner, I., Kudlacek, O., Choquet, D., Nanoff, C., Freissmuth, M. and Zezula, J.: A Two-State Model for the Diffusion of the A2A Adenosine Receptor in Hippocampal Neurons: Agonist-Induced Switch to Slow Mobility is Modified by Synapse-Associated Protein 102 (SAP102)., J Biol Chem 289, 9263-74, 2014
Rat	Rat, SD, embryonic	Hypothalamic neuronal	PDS kit: per instructions	Neurobasal A	Loktev, A. and Jackson, P.: Neuropeptide Y Family Receptors Traffic via the Bardet-Biedl Syndrome Pathway to Signal in Neuronal Primary Cilia., Cell Rep 5, 1316-29, 2013
Rat	Rat, E18	Hippocampal neurons	Papain: 0.2%	Hibernate	Todd, G., Boosalis, C., Burzycki, A., Steinman, M., Hester, L., Shuster, P. and Patterson, R.: Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons., PLoS ONE 8, e58996, 2013
Rat	Rat, SD, 175-200 g	Trigeminal ganglia	Papain: 20 u/ml Collagenase Type 2: 0.3%	HBSS	Yan, J., Melemedjian, O., Price, T. and Dussor, G.: Sensitization of Dural Afferents Underlies Migraine-Related Behavior Following Meningeal Application of Interleukin-6 (IL-6)., Mol Pain 8, 6, 2012
Rat	Rat, E19-21 and P1-3	Sympathetic neurons	Collagenase Type 2: 0.1% Neutral Protease: 0.5%	DMEM/F-12	Ghogha, A., Bruun, D. and Lein, P.: Inducing Dendritic Growth in Cultured Sympathetic Neurons., J Vis Exp 61, e3546, 2012
Rat	Rat, 20-30 day	Dorsal root ganglion	Papain: 20 u/ml Collagenase Type 1: 0.3% Neutral Protease: 0.4%	L-15	Bosmans, F., Puopolo, M., Martin-Eauclaire, M., Bean, B., Swartz, K.: Functional Properties and Toxin Pharmacology of a Dorsal Root Ganglion Sodium Channel Viewed through its Voltage Sensors., J Gen Physiol 138, 59, 2011
Rat	Rat, Wistar, E18	Neurons	Papain: 0.05%	Neurobasal	Giacomello, M., Girardi, S., Scorzeto, M., Peruffo, A., Maschietto, M., Cozzi, B. and Vassanelli, S.: Stimulation of Ca�+ Signals in Neurons by Electrically Coupled Electrolyte-Oxide-Semiconductor Capacitors., J Neurosci Methods 198, 1, 2011
Rat	Rat, SD, 200-300 g	Dorsal root ganglia, fibroblast	Collagenase: 0.125%	DMEM	East, E., de Oliveira, D., Golding, J. and Phillips, J.: Alignment of Astrocytes Increases Neuronal Growth in Three-Dimensional Collagen Gels and is Maintained Following Plastic Compression to Form a Spinal Cord Repair Conduit., Tissue Eng Part A 16, 3173, 2010
Rat	Rat, Fisher, 7-21 month	Hippocampal neurons	Papain: 0.2%	Hibernate A	Chen, N., Newcomb, J., Garbuzova-Davis, S., Davis Sanberg, C., Sanberg, P. and Willing, A.: Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro., Aging Dis 1, 173, 2010
Rat	Rat, SD, E18	Neuronal	Papain: 0.2%	Neurobasal E	Peltier, D., Simms, A., Farmer, J and Miller, D.: Human Neuronal Cells Possess Functional Cytoplasmic and TLR-Mediated Innate Immune Pathways Influenced by Phosphatidylinositol-3-Kinase Signaling, J Immunol 184, 7010, 2010
Rat	Rat, SD, 7 day	Cerebellar granule neurons	PDS kit: per instructions	PBS	Tanaka, S., Shaikh, I., Chiocca, E. and Saeki, Y.: The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development., PLoS ONE 4, e5922, 2009
Rat	Rat, SD, embryonic day 18	Hippocampal neurons	Papain: 20 u/ml	Neurobasal/B27	Liu, Y., Yohrling, G., Wang, Y., Hutchinson, T., Brenneman, D., Flores, C., Zhao, B.: Carisbamate, a Novel Neuromodulator, Inhibits Voltage-Gated Sodium Channels and Action Potential Firing of Rat Hippocampal Neurons., Epilepsy Res 83, 66, 2009
Rat	Rat, embryonic	Hypothalamic neurons and glias	Papain: 0.5 u/ml	DMEM	Yokosuka Makoto, Ohtani-Kaneko Ritsuko, Yamashita Kayoko, Muraoka Daisuke, Kuroda Yoichiro, Watanabe Chiho: Estrogen and environmental estrogenic chemicals exert developmental effects on rat hypothalamic neurons and glias, Toxicol In Vitro 22, 1-9, 2008
Rat	Rat, Wistar, 7 day	Superior cervical ganglion	Collagenase: 0.05%	L-15	Sakisaka, T., Yamamoto, Y., Mochida, S., Nakamura, M., Nishikawa, K., Ishizaki, H., Okamoto-Tanaka, M., Miyoshi, J., Fujiyoshi, Y., Manabe, T. and Takai, Y.: Dual Inhibition of SNARE Complex Formation by Tomosyn Ensures Controlled Neurotransmitter Release., J Cell Biol 183, 323, 2008
Rat	Rat, neonatal	Dopamine neurons	Papain: 20 u/ml	PBS	Frank, L., Caldera-Siu, A. and Pothos, E.: Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates., J Vis Exp 21, 820, 2008
Rat	Rat, Wistar, 4 day	Hippocampal neurons	PDS kit: per instructions	Neurobasal A	Obradovic Darja, Gronemeyer Hinrich, Lutz Beat, Rein Theo: Cross-talk of vitamin D and glucocorticoids in hippocampal cells, J Neurochem 96, 500-9, 2006
Rat	Rat, SD, neonatal	astrocytes	PDS kit: per instructions	DMEM	Lacroix-Fralish, M., Tawfik, V., Nutile-McMenemy, N., Harris, B. and Deleo, J.: Differential Regulation of Neuregulin 1 Expression by Progesterone in Astrocytes and Neurons., Neuron Glia Biol 2, 227, 2006
Rat	Rat, 2 month	Spinal cord progenitor cells Hybrid toxins	PDS kit: see reference	Neurobasal A	Mothe, A., Kulbatski, I., Van Bendegem, R., Lee, L., Kobayashi, E., Keating, A., and Tator, C.: Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells, J Histochem Cytochem 53(10), 1215, 2005
Rat	Rat, SD, E19	Dorsal root ganglia	PDS kit: per instructions	MEM/Ham's F12	Gavva NR, Tamir R, Qu Y, Klionsky L, Zhang TJ, Immke D, Wang J, Zhu D, Vanderah TW, Porreca F, Doherty EM, Norman MH, Wild KD, Bannon AW, Louis JC, Treanor JJ.: AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties, J Pharmacol Exp Ther 313, 474, 2005
Rat	Rat, Wistar, E15	Vomeronasal receptor neurons	Collagenase/Dispase: 0.1% Papain: 0.5 u/ml	DMEM/F12	Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M: Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons, Chem Senses 30, 111, 2005
Rat	Rat, SD, 1-2 day	Cortical astrocytes	Papain: see reference	DMEM	Floyd Candace L, Gorin Fredric A, Lyeth Bruce G: Mechanical strain injury increases intracellular sodium and reverses Na+/Ca2+ exchange in cortical astrocytes, Glia 51, 35-46, 2005
Rat	Rat, SD, 0-2 day	Superior cervical ganglion	Collagenase Type 4: 20 u/ml Trypsin: 0.25%	DMEM	Pedraza Carlos E, Podlesniy Petar, Vidal Noemi�, Arevalo Juan Carlos, Lee Ramee, Hempstead Barbara, Ferrer Isidre, Iglesias Montse, Espinet Carme: Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR, Am J Pathol 166, 533-43, 2005
Rat	Rat, SD, male, 5-8 week	Trigeminal neurons	Papain: 20 u/ml Collagenase: 0.3%	CMF Hanks	Connor, M, Naves, L. and McCleskey, E.: Contrasting Phenotypes of Putative Proprioceptive and Nociceptive Trigeminal Neurons Innervating Jaw Muscle in Rat., Mol Pain 1, 31, 2005
Rat	Rat, fetal	Brainstem and cortical neurons	PDS kit: per instructions	DMEM	Lovshin JA, Huang Q, Seaberg R, Brubaker PL, Drucker DJ: Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells, Endocrinology 145, 3495, 2004
Rat	Rat, SD, male 150-220 g	Dorsal root ganglion neurons	Collagenase Type 4: 0.125% Trypsin: 0.05%	DMEM/Ham's F12	Hu Hong-Zhen, Gu Qihai, Wang Chunbo, Colton Craig K, Tang Jisen, Kinoshita-Kawada Mariko, Lee Lu-Yuan, Wood Jackie D, Zhu Michael X: 2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3, J Biol Chem 279, 35741-8, 2004
Rat	Rat, 15 week	Neurons	Papain: 0.2%	Hibernate A	Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, and Shaw G: Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem, J Neurophysiol 87, 1076, 2002
Rat	Rat, SD, male, 270-330 g	Spinal progenitor cells	PDS kit: with modifications	Neurobasal medium	Lin CR, Wu PC, Shih HC, Cheng JT, Lu CY, Chou AK, Yang LC.: Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain, Cell Transplant 11, 17, 2002
Rat	Rat, Fisher, 8-9 week	Adult progenitor	Papain: 2.5 u/ml Deoxyribonuclease I: 250 u/ml Neutral Protease: 1 u/ml	DMEM/F-12	Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.: The adult substantia nigra contains progenitor cells with neurogenic potential, J Neurosci 22, 6639, 2002
Rat	Rat, SD, embryonic	Sciatic nerve and gut neural crest stem	Collagenase Type 4: 0.025% Trypsin: 0.005% Deoxyribonuclease I: 0.05%	HBSS	Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity., Neuron 35, 643, 2002
Rat	Rat	Neurons, hippocampal	Papain: 15 - 20 u/ml	Eagle's MEM (see reference)	Liu, Q., Kawai, H., and Berg, D.: B-Amyloid Peptide Blocks the Response of a7-Containing Nicotinic Receptors on Hippocampal Neurons, PNAS 98 (8), 4734, 2001
Rat	Rat, newborn, 7 days old	Neurons	Trypsin: 0.25%	MEM10	Acosta, C., Fabrega, A., Masco, D., and Lopez, H.: A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development, J Neurosci 21 (22), 8873, 2001
Rat	Rat, embryonic 18-19 day	Hippocampal neurons	PDS kit: see reference	DMEM	Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M, Matsumoto M: Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo, J Neurosci 21, 9204-13, 2001
Rat	Rat, embryonic, day 18	Cortical	Papain:	Neurobasal medium and DMEM	O'Connor, S., Andreadis, J., Shaffer, K., Ma, W., Pancrazio, J., and Stenger, D.: Immobilization of Neural Cells in Three-Dimensional Matrices for Biosensor Applications, Biosensors & Bioelectronics 14, 871, 2000
Rat	Rat, Wister, postnatal, P1-3 days	Neurons, hippocampal	Papain: 20 u/ml	EBSS	Neuhoff, H., Roeper, J., Schweizer, M.: Activity-Dependent Formation of Perforated Synapses in Cultured Hippocampal Neurons, Eur J Neurosci 11, 4241, 1999
Rat	Rat, SD, female, pregnant	Sciatic nerves	Trypsin: 0.025% Collagenase Type 3: 0.1%	L-15 medium (see reference)	Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell 96, 737, 1999
Rat	Rat (also mice)	Spinal cord	Trypsin: 0.133%	HBSS and PIPES	Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., and Frisen, J: Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System, Cell 96, 25, 1999
Rat	Rat, SD, embryonic	Sciatic nerves	Trypsin: 0.025% Collagenase Type 3: 0.1%	L-15 medium (see reference)	Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell 96, 737, 1999
Rat	Rat, (Long Evans), 2-5 day old	Neurons, hippocampal	Papain: 20 u/ml	EBSS	Wilding, T., and Huettner, J.: Activation and Desensitization of Hippocampal Kainate Receptors, J Neurosci 17 (8), 2713, 1997
Rat	Rat, Wister and SD, newborn, 0-21 days, either sex	Myenteric ganglia	Trypsin: 0.05%	MEM-HEPES	Schafer, K., Saffrey, M., Burnstock, G., and Mastres-Ventura, P.: A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract, Brain Res Proto 1, 109, 1997
Rat	Rat, pups, 24-48 h old	Neurons, hippocampal	Papain: 20 u/ml	Harvest buffer	Hall, R., and Soderling, T.: Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons, J Biol Chem 272 (7), 4135, 1997
Rat	Rat, SD, adult, 250-300g, P8	Neurons, DRG	Neutral Protease: 0.5%	L-15 w/ CO₂	Davies, S., Fitch, M., Memberg, S., Hall, A, Raisman, G., and Silver, J.: Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System, Nature 390, 680, 1997
Rat	Rat, 1-4 day old	Neurons, hippocampal	Papain: 20 u/ml	MEM	Twitchell, W., Brown, S., and Mackie, K.: Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons, J Neurophysiol 78, 43, 1997
Rat	Rat, SD, Fisher	Hippocampal neurons	Papain: 0.2%	HibernateA/B27	Brewer, G.J.: Isolation and culture of adult rat hippocampal neurons of any age, J Neurosci Methods 71, 143, 1997
Rat	Rat, E18	Hippocampal neurons	Papain: 20 u/ml Deoxyribonuclease I: 0.01%	MEM	Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL: Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons, J Neurosci 16, 2912-23, 1996
Rat	Rat, 1-6 day	DRG neurons	Papain: 20 u/ml Collagenase Type 1: 150 u/ml Neutral Protease: 0.8%	HBSS	Robertson, S., Rae, M., Rowan, E. and Kennedy, C.: Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia., Br J Pharmacol 118, 951, 1996
Rat	Rat, pups, 14 day old	Neurons, sympathetic	Neutral Protease: 0.24%	HBSS	McFarlane, S., and Cooper, E.: Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons, J Neurosci 13 (6), 2591, 1993
Rat	Rats, SD, 11-14 day old, 29-32 g	Basal forebrain neurons	Trypsin: 0.125%	Gey's BSS	Allen, T., Sim, J., and Brown, D.: The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat, J Physiol 460, 91, 1993
Rat	Rat, Wistar-Hanover, 7-12 day	Postnatal dopamine neurons	Trypsin: 0.035%	(see reference)	Rayport, S., Sulzer, D., Shi, W., Sawasdikosol, S., Monaco, J., Batson, D., and Rajendran, G.: Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology, J Neurosci 12 (11), 4264, 1992
Rat	Rat, SD, 18-day-old, fetus	Hippocampal	Trypsin: 0.2%	HBSS	Cheng, B., and Mattson, M.: IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage, J Neurosci 12 (4), 1558, 1992
Rat	Rat	Stem, neural crest	Collagenase: 0.075%	Ringer's solution	Stemple, D., and Anderson, D.: Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest, Cell 71, 973, 1992
Rat	Rat, SD, pregnant, Charles River	Pyramidal neurons Nonpyramidal neurons	Trypsin: 0.027%	HEPES	Buchhalter, J., and Dichter, M.: Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus, Brain Res Bull 26, 333, 1991
Rat	Rat, SD, embryos, 19-21 days gestation	Schwann, dorsal root ganglia	Trypsin: 0.25%	HBSS, CMF	Mithen, F., Reiker, M., and Birchem, R.: Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture, In Vitro Cell Dev Biol 26, 129, 1990
Rat	Rat (also bovine)	Heart Adrenal chromaffin Paraneurons	Trypsin: 0.06%	25mM HEPES buffered Locke's solution, CMF	Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990
Rat	Rat, SD, 8 day old pups	Cerebellar neurons	Trypsin: 0.025%	Eagle's MEM	Novelli, A., Reilly, J., Lysko, P., and Henneberry, R.: Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced, Brain Res 451, 205, 1988
Rat	Rat, fetuses, 18 day old	Hippocampal neurons	Trypsin: 0.2%	Eagle's MEM	Mattson, M., and Kater, S.: Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda, Int J Dev Neurosci 6 (5), 439, 1988
Rat	Rat, postnatal	Septal neurons	Papain: 0.05%	PBS, CMF	Hatanaka,H., Tsukui, H., Nihonmatsu, I.: Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor, Neurosci Lett 79, 85, 1987
Rat	Rat, Long Evans, 1-15 days	Neurons, visual cortex	Papain: 20 u/ml	BSS (see reference)	Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats, J Neurosci 6, 3044, 1986
Rat	Rat, SD, female, 4 month old	CNS cells	Trypsin: 0.25%	EBSS	Wood, P., and Bunge, R.: Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals, Nature 320, 756, 1986
Rat	Rat, embryo, 15 day	Dorsal horn neurons Spinal	Trypsin: 0.025%	Ham's F-12	Jahr, C. and Jessell, T.: Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate, J Neurosci 5, 2281, 1985
Rat	Rat, fetus, 18-20 day	Hippocampal neurons	Trypsin: 0.25%	HBSS, CMF	Bartlett, W. and Banker, G.: An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts, J Neurosci 4, 1944, 1984
Rat	Rat, postnatal	Ganglion, retina	Papain: 12.5 u/ml	HBSS w/5 mM HEPES	Leifer, D., Lipton, S., Barnstable, C., and Masland, R.: Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture, Science 224, 303, 1984
Rat	Rat, SD, pups	Retina	Trypsin: 0.25%	Ham's F-12	Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat, J Neurosci 3 (12), 2532, 1983
Rat	Rat, W/FU, 5-8 day	Neurons and glial	Trypsin: 0.25%	MEM (see reference)	Raff, M., Fields, K., Hakomori, S., Mirsky, R., Pruss, R., and Winter, J.: Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture, Brain Res 174, 283, 1979
Rat	Rat, fetus	Neurons, sympathetic	Trypsin: 0.25%	L-15 or HBSS, CMF	Wakshull, E., Johnson, M., Burton, H.: Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons, J Neurophysiol 42, 1410, 1979
Rat	Rat, Wistar/Furth, newborn (also bovine)	Schwann	Trypsin: 0.25%	DMEM	Brockes, J., Fields, K., and Raff, M.: Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve, Brain Res 165, 105, 1979
Rat	Rat, embryo	Neurons, cortical	Trypsin: 0.027%	MEM	Dichter, M.: Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation, Brain Res 149, 279, 1978
Rat	Rat, newborn	Neurons, sympathetic	Collagenase Type 1: 0.01%	Hank's solution, CF	Reichardt, L., Patterson, P.: Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures, Nature 270, 147, 1977
Rat	Rat, neonatal	Neurons, superior cervical ganglia	Trypsin: 0.1%	Basal L-15 medium	Mains, R., and Patterson, P.: Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties, J Cell Biol 59, 329, 1973
Salamander	Salamander, 18-25 cm	Retina	Papain: 14 u/ml	Saline	Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase, J Cell Biol 100, 175, 1985
Salamander	Salamander (A.tigrinum)	Photoreceptors, retina	Papain: 0.05%	(see reference)	Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina, Proc Natl Acad Sci U S A 75, 3507, 1978
Shellfish	Aplysia californica	Neurons	Neutral Protease: 1.0%	L-15-ASW	Lee, A., Decourt, B. and Suter, D.: Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones., J Vis Exp 12, 662, 2008
Shellfish	Snails (Helisoma trivolis), albino, adult	Buccal ganglia; SLT muscle	Trypsin: 0.2%	DMEM	Zoran, M., Doyle, R. and Haydon, P.: Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis, Neuron 6, 145, 1991
Shellfish	Pond snail (Helisoma) albino, adult	Somata, buccal ganglia	Trypsin: 0.2%	Antibiotic saline, Leibowitz 50%	Haydon, P.: The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata, J Neurosci 8, 1032, 1988
Shellfish	Helisoma trivovlis	Buccal ganglia	Trypsin: 0.2%	L-15 medium	Cohan, C., Haydon, P., and Kater, S.: Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma, J Neurosci Res 13, 285, 1985
Shellfish	Mollusc, juvenile 1-2 gm or adult 50-100 gm	Neurons LUQ cells	Protease: 1%	L15 medium	Schacher, S., and Proshanksy, E.: Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment, J Neurosci 3 (12), 2403, 1983
Shellfish	Aplysia californica	LUQ cells RUQ cells	Protease: 1%	L15 medium	Camardo, J., Proshansky, E., and Schacher, S.: Identified Aplysia Neurons Form Specific Chemical Synapses in Culture, J Neurosci 3 (12), 2614, 1983
Turtle	Turtle (Pseudemys scripta elegans)	Retinal	Papain: 0.1% (13.5 u/mg)	Kreb's Ringer	Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors, Proc Natl Acad Sci U S A 69, 1987, 1972

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