Prostate - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Human Human Prostatic cancer fibroblasts Collagenase: 225 u/ml
Hyaluronidase: 125 u/ml
RPMI 1640 Taylor, R., Toivanen, R., Frydenberg, M., Pedersen, J., Harewood, L., Collins, A., Maitland, N. and Risbridger, G.: Human Epithelial Basal Cells are Cells of Origin of Prostate Cancer, Independent of CD133 Status., Stem Cells 30, 1087, 2012
Human Human, 52-56 yr Prostatic stromal cells Collagenase Type 1: 0.2%
DMEM/F-12 Le Hanh, Arnold Julia T, McFann Kimberly K, Blackman Marc R: DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells, Am J Physiol/Endo 290, E952-60, 2006
Human Human Prostate stromal cells Collagenase Type 1: 0.1%
RPMI 1640 Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi: Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R, Am J Pathol 165, 533-40, 2004
Human Human, fetal Prostatic fibroblasts Collagenase Type 1: 0.125%
DMEM/F12 Levine AC, Liu XH, Greenberg PD, Eliashvili M, Schiff JD, Aaronson SA, Holland JF, Kirschenbaum A: Androgens induce the expression of vascular endothelial growth factor in human fetal prostatic fibroblasts, Endocrinology 139, 4672-8, 1998
Mouse Mouse, 6-8 week Prostate epithelial/stem Collagenase Type 2: 0.5%
Trypsin: 0.05%
HBSS Burger, P., Gupta, R., Xiong, X., Ontiveros, C., Salm, S., Moscatelli, D. and Wilson, E.: High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells., Stem Cells 27, 2220-8, 2009
Mouse Mouse, 2 week Prostatic epithelial Collagenase Type 3: 170 u/ml
DMEM Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake: Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate, Proc Natl Acad Sci U S A 101, 9375-80, 2004
Mouse Mouse, male Prostatic stem Collagenase Type 1: 170 u/ml
DMEM Dubey P, Wu H, Reiter RE, Witte ON: Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression, Cancer Res 61, 3256-61, 2001