Thymus - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Human Human Dentritic Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
 RPMI 1640 Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K: Human thymus contains 2 distinct dendritic cell populations, Blood 97, 1733-41, 2001
Mouse Mouse Thymic stromal Collagenase Type 4: 0.02%
Neutral Protease: 0.02%
Deoxyribonuclease I: 0.0025%
 RPMI-1640 Rode, I. and Boehm, T.: Regenerative Capacity of Adult Cortical Thymic Epithelial Cells., Proc Natl Acad Sci U S A 109, 3463-8, 2012
Mouse Mouse Stromal Collagenase Type 3: 0.2%
Hyaluronidase: 0.1%
 RPMI 1640 Phillips, Joy, Brondstetter, T., English, C., Lee, H., Virts, E, and Thoman, M.: IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution, J Immunol , 4869, 2004
Mouse Mouse Dentritic Collagenase Type 3: 0.1%
Deoxyribonuclease I: 325 u/ml
 RPMI 1640 Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196, 1415, 2002
Mouse Mouse, 3-6 week Thymic Collagenase Type 3: 100-400 u/ml
 HBSS Smith KM, Olson DC, Hirose R, Hanahan D: Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance, Int Immunol 9, 1355-65, 1997
Mouse Mouse (BALB/c, C3H, C57BL/6), 1-28 day Epithelial, thymus Neutral Protease: 1.5 µg/ml
 DMEM Ropke, C., van Deurs, B., and Petersen, O.: Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium, In Vitro Cell Dev Biol 26, 671, 1990
Mouse Mouse, C3H, 16 wk old, female Epithelial Collagenase Type 3: 0.1%
 DMEM Ehmann, U., Shiurba, R., and Peterson, W.: Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture, In Vitro Cell Dev Biol 22 (12), 738, 1986
Mouse Mouse, Swiss, 6 wk Thymus Collagenase Type 3: 150 u/ml
 DMEM Jones, K. and Pierre, R.: Analysis of Cellular Heterogeneity in Mouse Thymus Cultures, In Vitro 17, 431, 1981
Rat Rat, postnatal Thymic Trypsin: 0.05%
 HBSS Bonfanti, P., Claudinot, S., Amici, A., Farley, A., Blackburn, C. and Barrandon, Y.: Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells., Nature 466, 978, 2010
Rat Rat Thymic epithelial Collagenase Type 3: 0.1%
 DMEM Masuda, A and Matsuyama, M: Epithelial Cell Lines From Rat Thymoma and Rat Thymus,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 21C:4.1, 1995
Rat Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat, (ACI/MNs) male, 8 weeks
(also bovine, adult)		 Epthelial Collagenase Type 3: 0.1%
 Eagle's MEM Serum-free Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26, 713, 1990