Tumor - Tissue Tables

SpeciesSpecies DetailCellsEnzyme(s)Medium Reference
Hamster Hamster, 90-100 g Tumor Hyaluronidase: 0.1%
 Waymouth's MB Gonzalez, A., Oberley, T., Schultz, J., Ostrom, J., and Li, J.: In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney, In Vitro Cell Dev Biol 29A, 562, 1993
Hamster Hamster, 6 week old Buccal pouch Neutral Protease: 0.24%
 CMF HBSS Min, B., Kim, K., Cherrick, H., and Park, N.: Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation, In Vitro Cell Dev Biol 27A, 128, 1991
Human Human Tumor infiltrating lympocytes Collagenase Type 1: 200 u/ml
 RPMI Jeske, S., Schuler, P., Doescher, J., Theodoraki, M., Laban, S., Brunner, C., Hoffman, T. and Wigand, M.: Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma, Immun Ageing 17, 3, 2020
Human Human Tumor Collagenase Type 4: 200 u/ml
 DMEM Phan, N., Hong, J., Tofig, B., Mapua, M., Elashoff, D., Moatamed, N., Huang, J., Memarzadeh, S., Damoiseaux, R. and Soragni, A.: A Simple High-Throughput Approach Identifies Actionable Drug Sensitivities in Patient-Derived Tumor Organoids., Commun Biol 2, 78, 2019
Human Human Fibroblasts Collagenase Type 1: 0.5%
Neutral Protease: 0.1%
Deoxyribonuclease I: 1,000 u/ml
 FM Fibroblast media Ayuso, J., Vitek, R., Swick, A., Skala, M., Wisinski, K., Kimple, R., Lambert, P. and Beebe, D.: Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells, Sci Rep 9, 12480, 2019
Human Organoids Collagenase Type 4: 200-300 u/ml
 DMEM Neal, J,, Li ,X., Zhu, J., Giangarra, V., Grzeskowiak, C., Ju, J., Liu, I. Chiou, S., Salahudeen, A. and Smith, A. et al: Organoid Modeling of the Tumor Immune Microenvironment., Cell 175, 1972, 2018
Human Human Ameloblastoma epithelial Collagenase: 0.1%
Neutral Protease: 50 u/ml
 MEM Jiang, C., Zhang, Q., Shanti, R., Shi, S., Chang, T., Carrasco, L., Alawi, F. and Le, A.: Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells., Stem Cells 35, 2083-2094, 2017
Human Human, 25-81 yr Brain tumor Neutral Protease: 0.11 u/ml
Collagenase Type 4: 0.05%
Hyaluronidase: 1,000 u/ml
Deoxyribonuclease I: 5 u/ml
 HBSS Volovitz, I, Shapira, N., Ezer, H., Gafni, A., Lustgarten, M., Alter, T., Ben-Horin, I., Barzilai, O., Shahar, T., Kanner, A., Fried, I., Veshchev, I., Grossman, R. and Ram, Z,: A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells., BMC Neurosci 17, 30, 2016
Human Human Pancreatic cancer Stemxyme 1: 0.2%
 HBSS Pham, K., Delitto, D., Knowlton, A., Hartlage, E., Madhavan, R., Gonzalo, D., Thomas, R., Behrns, K., George, T., Hughes, S., Wallet, S., Liu, C., Trevino, J.: Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture., Am J Pathol 186, , 2016
Human Human Non-small cell lung tumor Collagenase Type 1: 0.1%
Collagenase Type 2: 0.25%
 RPMI-1640 Liang, S., Marti, T., Dorn, P., Froment, L., Hall, S., Berezowska, S., Kocher, G., Schmid, R. and Peng, R.: Blocking the Epithelial-to-Mesenchymal Transition Pathway Abrogates Resistance to Anti-Folate Chemotherapy in Lung Cancer., Cell Death Dis 6, e1824, 2015
Human Human Lung tumor Collagenase Type 1: 45-60 u/ml
Collagenase Type 2: 15-20 u/ml
Collagenase Type 4: 45-60 u/ml
Elastase: 0.002%
Deoxyribonuclease I: 0.002%
 DMEM/F12 Quatromoni, J., Singhal, S., Bhojnagarwala, P., Hancock, W., Albelda, S. and Eruslanov, E.: An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells, J Leukoc Biol 97, 201, 2015
Human Human Tumour infiltrating lymphocytes Collagenase: 1%
Hyaluronidase: 1%
Deoxyribonuclease I: 3,000 u/ml
 RPMI 1640 Baldan, V., Griffiths, R., Hawkins, R. and Gilham, D: Efficient and Reproducible Generation of Tumour-Infiltrating Lymphocytes for Renal Cell Carcinoma, Br J Cancer 112, 1510, 2015
Human Human Colon Trypsin: 0.1%
Collagenase: 0.1%
 HBSS Ali, M., Anand, S., Tangella, K., Ramkumar, D. and Saif, T.: Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry., J Vis Exp , e52532, 2015
Human Human Glioblastoma tumor Trypsin: 0.025%
Collagenase Type 4: 500 u/ml
 HBSS Hasselbach, L., Irtenkauf, S., Lemke, N., Nelson, K., Berezovsky, A., Carlton, E., Transou, A., Mikkelsen, T. and deCarvalho, A.: Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry., J Vis Exp , e51088, 2014
Human Human Glioma cancer stem PDS kit: per instructions
 Neurobasal Yan, K., Wu, Q., Yan, D., Lee, C., Rahim, N., Tritschler, I., DeVecchio, J., Kalady, M., Hjelmeland, A. and Rich, J.: Glioma Cancer Stem Cells Secrete Gremlin1 to Promote their Maintenance Within the Tumor Hierarchy., Genes Dev 28, 1085-100, 2014
Human Human Melanoma Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.01%
 PBS Welte, Y., Davies, C., Schafer, R. and Regenbrecht, C.: Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma., J Vis Exp 73, e50200, 2013
Human Human Tumor Collagenase Type 1: 0.1%
Hyaluronidase: 0.01%
Deoxyribonuclease I: 0.01%
 DMEM/F12 Chou, J., Fitzgibbon, M., Mortales, C., Towlerton, A., Upton, M., Yeung, R., McIntosh, M. and Warren, E.: Phenotypic and Transcriptional Fidelity of Patient-Derived Colon Cancer Xenografts in Immune-Deficient Mice., PLoS ONE 8, e79874, 2013
Human Human Cancer stem Trypsin: 0.25%
Collagenase: 0.05%
Hyaluronidase: 0.005%
 RPMI Dobbin, Z. and Landen, C.: Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications., Curr Protoc Pharmacol 63, Unit 14.28., 2013
Human Human Colorectal cancer Collagenase Type 4: 2%
Deoxyribonuclease I: 0.1%
 RPMI 1640 Zhou, J, Belov, L., Solomon, M., Chan, C., Clarke, S. and Christopherson, R.: Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing., J Vis Exp 55, e3322, 2011
Human Human Brain tumor Collagenase Type 1: 0.04%
Hyaluronidase: 0.01%
Deoxyribonuclease I: 0.02%
Neutral Protease: 0.008%
 DMEM/F12 Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.: Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion., Neuro Oncol 13, 70-83, 2011
Human Human Tumorigenic melanoma Collagenase Type 4: 200 u/ml
Trypsin: 0.05%
Deoxyribonuclease I: 50-100 u/ml
 PBS Quintana, E., Shackleton, M., Foster, H., Fullen, D., Sabel, M., Johnson, T. and Morrison, S.: Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized., Cancer Cell 18, 510, 2010
Human Human Pancreatic tumor Collagenase Type 4: 200 u/ml
 RPMI-1640 Kim, M., Evans, D., Wang, H., Abbruzzese, J., Fleming, J. and Gallick, G.: Generation of Orthotopic and Heterotopic Human Pancreatic Cancer Xenografts in Immunodeficient Mice., Nat Protoc 4, 1670, 2009
Human Human Tumor Collagenase Type 4: 0.1%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.04%
 see reference Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.: Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.,Neuro Oncol Vol. 11,, , 109, 2009
Human Human Colon cancer Collagenase Type 1: 300 u/ml
Hyaluronidase: 100 u/ml
 DMEM/F12 Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion., EMBO Mol Med 1, 338-51, 2009
Human Human Breast epithelial Collagenase Type 3: 200 u/ml
 HBSS Liu, R., Wang, X., Chen, G., Dalerba, P., Gurney, A., Hoey, T., Sherlock, G., Lewicki, J., Shedden, K. and Clarke, M.: The Prognostic Role of a Gene Signature from Tumorigenic Breast-Cancer Cells., N Engl J Med 356, 217, 2007
Human Human Prostate stromal cells Collagenase Type 1: 0.1%
 RPMI 1640 Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi: Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R, Am J Pathol 165, 533-40, 2004
Human Human, adult Human synovial sarcoma Collagenase Type 2: 200 u/ml
 DMEM/F-12 Nishio Jun, Iwasaki Hiroshi, Ishiguro Masko, Ohjimi Yuko, Fujita Chikako, Isayama Teruto, Naito Masatoshi, Oda Yoshinao, Kaneko Yasuhiko, Kikuchi Masahiro: Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor, Int J Oncol 21, 17-23, 2002
Human Human Colonocytes Collagenase: 0.15%
 DMEM/F12 Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes, J Nutr 46, 96-105, 2001
Human Human Epithelial, fibroblasts Trypsin: 0.25%
 Ham's F-12 MacLeod, R: Rapid Monolayer Primary Cell Culture from Tissue Biopsy,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons Ltd, 3E:2.1, 1995
Human Human Colon adenocarcinoma Hyaluronidase: 100 u/ml
 DMEM Hague, A and Paraskeva, C: Colon Adenocarcinoma Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12C:1.1, 1995
Human Human Tumor, breast Hyaluronidase: 100 u/ml
 DMEM Beaupain, R., Mainquene, C., Brouty-Boye, D., Planchon, P., and Magniew, V.: "Normal" Breast Cells Adjacent to a Tumor Grown in Long-term Three Dimensional Culture, In Vitro Cell Dev Biol 29, 100, 1993
Human Human Glioma Hyaluronidase: 0.01%
 HBSS Kruse, C., Mitchell, D., Kleinschmidt-DeMasteis, B., Franklin, W., Morse, H., Spector, E., and Lillehei, K.: Characterization of a Continuous Human Glioma Cell Line DBTRG-OSMG: Growth Kinetics, Karyotype, Receptor Expression and Tumor Suppressor Gene Analyses, In Vitro Cell Dev Biol 28, 609, 1992
Human Human Epithelial Collagenase: 2.0%
 DMEM/Ham's F-12 Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples, In Vitro Cell Dev Biol 26, 1186, 1990
Human Human Tumor Neutral Protease: 0.24%
 DMEM/Ham's F-12 Boyd, J., Rinehart Jr., C., Walton, L., Siegal, G. and Kaufman, D.: Ultrastructural Characterization of Two New Human Endometrial Carcinoma Cell Lines and Normal Human Endometrial Epithelial Cells Cultured on Extracellular Matrix, In Vitro Cell Dev Biol 26, 701, 1990
Human Human, 9-74 year Neurofibroma Neutral Protease: 1.25 u/ml
Collagenase Type 1: 0.05%
Hyaluronidase: 0.1%
 L-15 Sheela S, Riccardi VM, Ratner N: Angiogenic and invasive properties of neurofibroma Schwann cells, J Cell Biol 111, 645-53, 1990
Human Human Tumor Trypsin: 0.05%
 DMEM Sacks, P., Parnes, S., Gallick, G., Mansouri, Z., Lichtner, R., Satya-Prakash, K., Pathak, S, and Parsons, D.: Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck, Cancer Res 48, 2858, 1988
Human Human Tumor, colon Trypsin: 0.25%
 McCoy's Brattain, M., Marks, M., McCombs, J., Finely, W., and Brattain, D.: Characterization of Human Colon Carcinoma Cell Lines Isolated From a Single Primary Tumour, Br J Cancer 47, 373, 1983
Human Human Epthelial and tumor
Colon		 Collagenase: 300 u/ml
 PBS medium 199 or medium F 12 Friedman, E., Higgins, P., Lipkin, M., Shinya, H., and Gelb, A.: Tissue Culture of Human Epithelial Cells from Benign Colonic Tumors, In Vitro 17, 632, 1981
Human Human Tumor, breast Neuraminidase: 0.8 u/ml
 HBSS Leung, C., and Shiu, R.: Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix, In Vitro 18, 476, 1981
Human Human Melanoma
Metastatic tumors		 Collagenase Type 3: 0.10%
 DMEM Creasey, A., Smith, H., Hackett, A., Fukuyama, K., Epstein, W., and Madin, S.: Biological Properties of Human Melanoma Cells in Culture, In Vitro 15, 342, 1979
Human Human Mammary tumors, hard Collagenase: 0.10%
 RPMI-1640 w/ 5% Fetal Calf Serum Lasfargues, E.: Human Mammary Tumors,Tissue Culture Methods/Applications,Kruse, P., and Patterson, M., Academic Press, 45, 1973
Mouse Mouse liver tumor Collagenase Type 2: 0.25%
 HBSS Walker, S., Wankell, M., Ho, V., White, R., Deo, N., Devine, C., Dewdney, B., Bhathal, P., Govaere, O., Roskams, T., Qiao, L., George, J. and Hebbard, L.: Targeting mTOR and Src Restricts Hepatocellular Carcinoma Growth in a Novel Murine Liver Cancer Model., PLoS ONE 14, e0212860, 2019
Mouse Mouse, adult Organoids Collagenase Type 4: 200-300 u/ml
 DMEM Neal, J,, Li ,X., Zhu, J., Giangarra, V., Grzeskowiak, C., Ju, J., Liu, I. Chiou, S., Salahudeen, A. and Smith, A. et al: Organoid Modeling of the Tumor Immune Microenvironment., Cell 175, 1972, 2018
Mouse Mouse Tumor derived neutroblastoma Papain: 10 u/ml
Deoxyribonuclease I: 250 u/ml
 DPBS Erdreich-Epstein, A., Singh, A., Joshi, S., Vega, F., Guo, P., Xu, J., Groshen, S., Ye, W., Millard, M., Campan, M., Morales, G., Garlich, J., Laird, P., Seeger, R., Shimada, H. and Durden, D.: Association of High Microvessel A,B and Low PTEN with Poor Outcome in Stage 3 Neuroblastoma: Rationale for Using First in Class Dual PI3K/BRD4 Inhibitor, SF1126., Oncotarget 8, 52193-52210, 2017
Mouse Mouse Glioblastoma PDS kit: per instructions
 DMEM/F12 Boyd, N., Walker, K., Fried, J., Hackney, J., McDonald, P., Benavides, G., Spina, R., Audia, A., Scott, S., Libby, C., Tran, A., Bevensee, M., Griguer, C., Nozell, S., Gillespie, G. and Nabors, B.: Addition of Carbonic Anhydrase 9 Inhibitor SLC-0111 to Temozolomide Treatment Delays Glioblastoma Growth In Vivo., JCI Insight 2, , 2017
Mouse Mouse Tumor Collagenase Type 4: 0.1%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.02%
 RPMI-1640 Filtjens, J., Keirsse, J., Van Ammel, E., Taveirne, S., Van Acker, A., Kerre, T., Taghon, T., Vandekerckhove, B., Plum, J., Van Ginderachter, J. and Leclercq, G.: Expression of the Inhibitory Ly49E Receptor is Not Critically Involved in the Immune Response Against Cutaneous, Pulmonary or Liver tumours., Sci Rep 6, 30564, 2016
Mouse Mouse Tumor Collagenase: 0.1%
Neutral Protease: 0.5 u/ml
 F12K Aguilera, T., Rafat, M., Castellini, L., Shehade, H., Kariolis, M., Hui, A., Stehr, H., von Eyben, R., Jiang, D., Ellies, L., Koong, A., Diehn, M., Rankin, E. Graves, E. and Giaccia, A.: Reprogramming the Immunological Microenvironment through Radiation and Targeting Axl., Nat Commun 7, 13898, 2016
Mouse Mouse Tumor-infiltrating lymphocytes Collagenase Type 4: 0.25%
 DMEM Singer, M., Wang, C., Cong, L., Marjanovic, N., Kowalczyk, Mo., Zhang, H., Nyman, J., Sakuishi, K., Kurtulus, S., Gennert, D., Xia, J., Kwon, J., Nevin, J., Herbst, R., Yanai, I., Rozenblatt-Rosen, O. and Kuchroo, V.: A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells., Cell 166, 1500-1511.e9, 2016
Mouse Mouse Glioblastoma PDS kit: per instructions
 Neurobasal Otvos, B., Silver, D., Mulkearns-Hubert, E., Alvarado, A., Turaga, S., Sorensen, M., Rayman, P., Flavahan, W., Hale, J., Stoltz, K., Sinyuk, M., Wu, Q., Jarrar, A., Kim, S., Fox, P., Nakano, I. and Rich, J.: Cancer Stem Cell-Secreted Macrophage Migration Inhibitory Factor Stimulates Myeloid Derived Suppressor Cell Function and Facilitates Glioblastoma Immune Evasion., Stem Cells 34, 2026-39, 2016
Mouse Mouse Keratinocytes Trypsin: 0.25%
Collagenase Type 1: 0.12%
Collagenase Type 2: 0.05%
Collagenase Type 4: 0.05%
Hyaluronidase: 0.1%
 Low calcium containing media Blanco, S., Bandiera, R., Popis, M., Hussain, S., Lombard, P., Aleksic, J., Sajini, A., Tanna, H., Cortes-Garrido, R., Gkatza, N., Dietmann, S. and Frye, M.: Stem Cell Function and Stress Response are Controlled by Protein Synthesis., Nature 534, 335-40, 2016
Mouse Mouse Mammary tumor Collagenase Type 1: 200 u/ml
Hyaluronidase: 0.001%
 DMEM/F12 Hosseini, H., Obradovic, M., Hoffmann, M., Harper, K., Sosa, M., Werner-Klein, M., Nanduri, L., Werno, C., Ehrl, C., Maneck, M., Patwary, N., Haunschild, G., Guzvic, M., Reimelt, C. et al.: Early Dissemination Seeds Metastasis in Breast Cancer., Nature , , 2016
Mouse Mouse Endothelial Collagenase Type 2: 0.1%
Neutral Protease: 0.25 u/ml
Deoxyribonuclease I: 0.0075%
 DMEM Xiao, L., McCann, J. and Dudley, A.: Isolation and Culture Expansion of Tumor-specific Endothelial Cells., J Vis Exp , e53072, 2015
Mouse Mouse Thyroid tumor Collagenase Type 1: 300 u/ml
Hyaluronidase: 100 u/ml
Deoxyribonuclease I: 0.01%
 DMEM Kitajima, S., Kohno, S., Kondoh, A., Sasaki, N., Nishimoto, Y., Li, F., Mohammed, M., Muranaka, H., Nagatani, N., Suzuki, M., Kido, Y. and Takahashi, C.: Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts., Stem Cells 33, 1657-69, 2015
Mouse Mouse Tumor Collagenase Type 4: 0.1
Hyaluronidase: 0.01%
Deoxyribonuclease I: 30 u/ml
 PBS Devaud, C., Westwood, J., John, L., Flynn, J., Paquet-Fifield, S., Duong, C., Yong, C., Pegram, H., Stacker, S., Achen, M. Stewart, T., Snyder, L., Teng, M., Smyth, M., Darcy, P. and Kershaw, M: Tissues in Different Anatomical Sites can Sculpt and Vary the Tumor Microenvironment to Affect Responses to Therapy., Mol Ther 22, 18-27, 2014
Mouse Mouse Tumor Collagenase Type 1: 250 u/ml
 DMEM Ali, O., Verbeke, C., Johnson, C., Sands, R., Lewin, S., White, D., Doherty, E., Dranoff, G. and Mooney, D.: Identification of Immune Factors Regulating Antitumor Immunity Using Polymeric Vaccines with Multiple Adjuvants., Cancer Res 74, 1670-81, 2014
Mouse Mouse Tumor Collagenase Type 2: 0.2%
Neutral Protease: 2.5 u/ml
Deoxyribonuclease I: 0.0075%
 DMEM Dunleavey, J., Xiao, L., Thompson, J., Kim, M., Shields, J., Shelton, S., Irvin, D., Brings, V., Ollila, D., Brekken, R., Dayton, P., Melero-Martin, J. and Dudley, A.: Vascular Channels Formed by Subpopulations of PECAM1+ Melanoma Cells., Nat Commun 5, 5200, 2014
Mouse Mouse Fibroblasts Collagenase Type 2: 0.25%
Collagenase Type 4: 0.25%
Deoxyribonuclease I: 0.05%
 DMEM Sharon, Y., Alon, L., Glanz, S., Servais, C. and Erez, N.: Isolation of Normal and Cancer-Associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS)., J Vis Exp 71, e4425, 2013
Mouse Mouse Prostate tumor Collagenase Type 4: 1,600 u/ml
 DMEM/F12 Mazzoleni, S., Jachetti, E., Morosini, S., Grioni, M., Piras, I., Pala, M., Bulfone, A., Freschi, M., Bellone, M. and Galli, R.: Gene Signatures Distinguish Stage-Specific Prostate Cancer Stem Cells Isolated From Transgenic Adenocarcinoma of the Mouse Prostate Lesions and Predict the Malignancy of Human Tumors., Stem Cells Transl Med 2, 678, 2013
Mouse Mouse Mammary tumor Collagenase: 0.15%
Hyaluronidase: 0.020%
 DMEM/F12 Liu, X., Johnson, S., Liu, S., Kanojia, D., Yue, W., Singh, U., Wang, Q, Wang Qi, Nie, Q. and Chen H.: Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy., Sci Rep 3, 2473, 2013
Mouse Mouse Rhabdomycosarcoma tumor Trypsin: 0.012%
Collagenase Type 2: 0.1%
 DMEM Chen, X., Stewart, E., Shelat, A., Qu, C., Bahrami, A., Hatley, M., Wu, G., Bradley, C., McEvoy, J., Pappo, A., Spunt, S., Valentine, M., Valentine, V., Krafcik, F., Lang, W., Wierdl, M. and Tsurkan, L.: Targeting Oxidative Stress in Embryonal Rhabdomyosarcoma., Cancer Cell 24, 710-24, 2013
Mouse Mouse Tumor endothelial Collagenase Type 1: 0.2%
 HBSS Kazerounian, S., Gerald, D., Huang, M., Chin, R, Udayakumar, D, Zheng, N., O'Donnell, R., Perruzzi, C., Mangiante, L., Pourat, J., Phung, T., Bravo-Nuevo, A., Shechter, S., McNamara, S. and Duhadaway, K.: RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis., Cancer Res 73, 50, 2013
Mouse Mouse, male Dendritic, macrophages Collagenase (1 or 4): 100-200 u/ml
Deoxyribonuclease I: 0.01%
 RPMI Watkins, S., Zhu, Z., Watkins, K. and Hurwitz, A.: Isolation of Immune Cells from Primary Tumors., J Vis Exp 64, e3952, 2012
Mouse Mouse Lung tumor Neutral Protease: 50 u/ml
Collagenase: 400 u/ml
Deoxyribonuclease I: 50 u/ml
 DMEM Vaughan, A., Halbert, C., Wootton, S. and Miller, A.: Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation., Mol Cancer Res 10, 86, 2012
Mouse Mouse Macrophages Collagenase Type 4: 0.2%
 PBS Hamzah, J., Kotamraju, V., Seo, J., Agemy, L., Fogal, V., Mahakian, L., Peters, D., Roth, L., Gagnon, M., Ferrara, K. and Ruoslahti, E.: Specific Penetration and Accumulation of a Homing Peptide Within Atherosclerotic Plaques of Apolipoprotein E-deficient Mice., Proc Natl Acad Sci U S A 108, 7154, 2011
Mouse Mouse Pancreatic cancer stem Collagenase Type 4: 200 u/ml
Neutral Protease: 0.6 u/ml
 DMEM Rasheed, Z., Wang, Q. and Matsui, W.: Isolation of Stem Cells from Human Pancreatic Cancer Xenografts., J Vis Exp 43, 2169, 2010
Mouse Mouse Tumor infiltration lymphocytes Collagenase Type 1: 0.25%
Collagenase Type 2: 0.15%
Collagenase Type 4: 0.1%
Hyaluronidase: 0.025%
 RPMI 1640 Kwong, B., Roberts, S., Silberzahn, T., Filler, R., Neustadter, J., Galan, A., Reddy, S., Lin, W., Ellis, P., Langford, C., Hayday, A. and Girardi, M.: Molecular Analysis of Tumor-Promoting CD8+ T cells in Two-Stage Cutaneous Chemical Carcinogenesis., J Invest Dermatol 130, 1726, 2010
Mouse Mouse, 4-6 week Pancreatic tumor Collagenase Type 4: 200 u/ml
 RPMI-1640 Kim, M., Evans, D., Wang, H., Abbruzzese, J., Fleming, J. and Gallick, G.: Generation of Orthotopic and Heterotopic Human Pancreatic Cancer Xenografts in Immunodeficient Mice., Nat Protoc 4, 1670, 2009
Mouse Mouse Tumor Collagenase Type 3: 200 u/ml
 RPMI-1640 Prince, M., Sivanandan, R., Kaczorowski, A., Wolf, G., Kaplan, M., Dalerba, P., Weissman, I., Clarke, M. and Ailles, L.: Identification of a Subpopulation of Cells with Cancer Stem Cell Properties in Head and Neck Squamous Cell Carcinoma., Proc Natl Acad Sci U S A 104, 973, 2007
Mouse Mouse, 30 week Tumor endothelial Collagenase Type 4: 500 u/ml
Collagenase Type 2: 550 u/ml
Deoxyribonuclease I: 3 u/ml
 PBS Berger, M., Bergers, G., Arnold, B., Hammerling, G. and Ganss, R.: Regulator of G-protein Signaling-5 Induction in Pericytes Coincides With Active Vessel Remodeling During Neovascularization, Blood 105, 1094, 2005
Mouse Mouse Granule cell precursors, pre-neoplastic and tumor cells Papain: 10 u/ml
Deoxyribonuclease I: 250 u/ml
 Neurobasal/B27 Oliver, T., Read, T., Kessler, J., Mehmeti, A., Wells, J., Huynh, T., Lin, S. and Wechsler-Reya, R.: Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma., Development 132, 2425, 2005
Mouse Mouse, 8-10 week Tumor associated endothelial cells Collagenase Type 2:
see reference Hida Kyoko, Hida Yasuhiro, Amin Dhara N, Flint Alan F, Panigrahy Dipak, Morton Cynthia C, Klagsbrun Michael: Tumor-associated endothelial cells with cytogenetic abnormalities, Cancer Res 64, 8249-55, 2004
Mouse Mouse Pancreatic tumor Collagenase Type 2: 0.5%
Collagenase Type 4: 0.5%
Deoxyribonuclease I: 0.2%
 PBS Bergers Gabriele, Song Steven, Meyer-Morse Nicole, Bergsland Emily, Hanahan Douglas: Benefits of targeting both pericytes and endothelial cells in the tumor vasculature with kinase inhibitors, J Clin Invest 111, 1287-95, 2003
Mouse Mouse Tumor-infiltrating lymphocyte Collagenase: 200 u/ml
 RPMI 1640 Uekusa Yasuhiro, Yu Wen-Gong, Mukai Takao, Gao Ping, Yamaguchi Nobuya, Murai Masako, Matsushima Kouji, Obika Satoshi, Imanishi Takeshi, Higashibata Yuji, Nomura Shintaro, Kitamura Yukihiko, Fujiwara Hiromi, Hamaoka Toshiyuki: A pivotal role for CC chemokine receptor 5 in T-cell migration to tumor sites induced by interleukin 12 treatment in tumor-bearing mice, Cancer Res 62, 3751-8, 2002
Mouse Mouse, male Melanoma tumor cells Collagenase Type 2: 0.5%
 DMEM Arbiser JL, Raab G, Rohan RM, Paul S, Hirschi K, Flynn E, Price ER, Fisher DE, Cohen C, Klagsbrun M: Isolation of mouse stromal cells associated with a human tumor using differential diphtheria toxin sensitivity, Am J Pathol 155, 723-9, 1999
Mouse Mouse (BALB/cfC3H) Mammary tumors
Epithelial		 Collagenase: 1.0%
 HBSS Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels, In Vitro 16, 502, 1980
Mouse Mouse, BALB/cfC3H/Crgl Neoplastic
Epithelial tumor		 Trypsin:
DMEM Hosick, H.: A Note on Growth Patterns of Epithelial Tumor Cells in Primary Culture, Cancer Res 34, 259, 1974
Mouse Mouse, lactating, 14 day Mammary Trypsin NF 1:250: 0.25%
 HBSS Kopelovich, L., Abraham, S., McGrath, H., DeOme, K., Chaikoff, I.: Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland, Cancer Res 26, 1534, 1966
Rat Rat, adult Tumor Collagenase Type 1: 0.16%
Hyaluronidase: 0.002%
Deoxyribonuclease I: 0.006%
 DMEM/F12 Duarte, S., Momier, D., Baque, P., Casanova, V., Loubat, A., Samson, M., Guigonis, J., Staccini, P., Saint-Paul, M., De Lima, M., Carle, G. and Pierrefite-Carle, V.: Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model., Stem Cells 31, 423-32, 2013
Rat Rat Hippocampal neurons Papain: 0.2%
 Hibernate A Mehta, N., Lopez, P., Vyas, A. and Schnaar, R.: Gangliosides and Nogo Receptors Independently Mediate Myelin-Associated Glycoprotein Inhibition of Neurite Outgrowth in Different Nerve Cells., J Biol Chem 282, 27875-86, 2007
Rat Rat, male 12 week Sponge infiltrating cells Collagenase Type 4: 0.15%
Deoxyribonuclease I: 0.02%
 RPMI 1640 Sharma N, Luo J, Kirschmann DA, O'Malley Y, Robbins ME, Akporiaye ET, Lubaroff DM, Heidger PM, Hendrix MJ: A novel immunological model for the study of prostate cancer, Cancer Res 59, 2271-6, 1999
Rat Rat (ACI/NMs X BUF/Mna) F1, male, 28 months, rat, (ACI/MNs) male, 8 weeks Epthelial Collagenase Type 3: 0.1%
 Eagle's MEM Serum-free Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26, 713, 1990
Rat Rat Yolk sac tumor Trypsin: 0.01%
 DMEM Brennan, M., Oldberg, A., Hayman, E., and Ruoslahti, E.: Effect of a Proteoglycan Produced by Rat Tumor Cells on Their Adhesion to Fibronectin-Collagen Substrata, Cancer Res 43, 4302, 1983
Rat Rat, SD, female Epithelial, cancer and tumor Collagenase: 0.1%
 Eagles's MEM Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma, In Vitro 18, 565, 1982
Rat Rat Tumor, islet Trypsin: 0.05%
 Medium 199 Gazdar, A., Chick, W., Oie, H., Sims, H., King, D., Weir, G., and Lauris, V.: Continuous, Clonal, Insulin-and Somatostatin-Secreting Cell Lines Established from a Transplantable Rat Islet Cell Tumor, Proc Natl Acad Sci U S A 77 (6), 3519, 1980
Rat Rat Ascites hepatoma Trypsin: 0.1%
 Phosphate buffer (see reference) Essner, E.: Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands, Exp Cell Res 7, 430, 1954