Tissue Dissociation References
Tissue Type: Uterus

Uterus: Mouse
  • BALB/c mice
Bigsby, R., Cooke, P., and Cunha, G.: A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells, Am J Physiol 251, E630, 1986
Cells:     Epithelial cellsMesencymal cells
Enzyme(s)    Trypsin: 1%
Soybean Trypsin Inhibitor: 1%
Deoxyribonuclease: 0.1%
Medium    DMEM

Uterus: Mouse
  • 40 day old BALB/cCrgl mice
Cooke, P., Uchima, F., Fujii, D., Bern, H., and Cunha, G.: Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma, Proc Natl Acad Sci U S A 83, 2109, 1986
Cells:     Epithelial cells
Enzyme(s)    Collagenase Type III: 0.1%
Trypsin: 5 mg
Medium    Medium 199

Uterus: Mouse
  • 21-23 day old outbread CD-1 mice
Tomooka, Y., DiAugustine, R., and McLachlan, J.: Proliferation of Mouse Uterine Epithelial Cells in Vitro, Endocrinology 118 (3), 1011, 1986
Cells:     Epithelial cells
Enzyme(s)    Trypsin
Medium    Medium 199

Uterus: Rat
  • Female, SD rats
Glasser, S., Julian, J., Decker, G., Tang, J., and Carson, D.: Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells, J Cell Biol 107 (6), 2409, 1988
Cells:     Luminal epithelial cells
Enzyme(s)    Trypsin
Medium    HBSS

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