Tissue dissociation/primary cell isolation and cell harvesting are principal applications for enzymes in tissue culture research and cell biology studies.

Researchers searching the scientific literature for information on the ideal enzymes and optimal conditions for tissue dissociation are often confronted with conflicting data. Much of this variation stems from the complex and dynamic nature of the extracellular matrix and the use of relatively crude, undefined enzyme preparations.

The Worthington Tissue Dissociation Guide summarizes many tissue-specific references and presents an organized system to establish a cell isolation protocol. In general, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.

For additional information, see our full website.