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| Tissue Dissociation References |
| Tissue Guide Home Page | |
Adipose/Fat: Avian | |
| Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges
, Methods Mol Biol 1345,
299, 2016 | |
| Cells: | Amyloid Fibrils |
| Enzyme(s) | Collagenase Type 3: 0.02% Deoxyribonuclease I: 0.001% |
| Medium | 50mM Tris |
Adipose/Fat: Avian | |
| Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges
, Methods Mol Biol 1345,
299, 2016 | |
| Cells: | Amyloid Fibrils |
| Enzyme(s) | Collagenase Type 3: 0.02% Deoxyribonuclease I: 0.001% |
| Medium | 50mM Tris |
Adipose/Fat: Bear | |
| |
| Gehring, J., Rigano, K., Evans, H., Nelson, O., Robbins, C. and Jansen, H.: A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes.
, Cytotechnology
68,
2177-91, 2016 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Adipose/Fat: Bovine | |
| |
| Yang, Y., Baldwin, R.: Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue
, J Dairy Sci 56,
350, 1973 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 40 u/ml |
| Medium | Krebs-Ringer bicarbonate |
Adipose/Fat: Canine | |
| |
| Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts.
, Tissue Eng Part A
19,
1295-302, 2013 | |
| Cells: | Stromal vascular |
| Enzyme(s) | Collagenase: see reference |
| Medium | Media 199E |
Adipose/Fat: Canine | |
| |
| Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.
, Lipids Health Dis
10,
171, 2011 | |
| Cells: | Renal adipose derived cells |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Adipose/Fat: Canine | |
| |
| Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force.
, J Surg Res 152,
157, 2009 | |
| Cells: | Adipose stem cells |
| Enzyme(s) | Collagenase: see reference |
| Medium | Media-199 |
Adipose/Fat: Canine | |
| |
| DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species
, Am J Physiol 221,
850, 1971 | |
| Cells: | White fat |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Kreb's Ringer bicarbonate buffer |
Adipose/Fat: Equine | |
| |
| Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow.
, Vet Surg
37,
713, 2008 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Equine | |
| |
| Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells.
, Vet Surg
36,
613, 2007 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Fish | |
| |
| Todorcevic, M., Vegusdal, A., Gjoen, T., Sundvold, H., Torstensen, B., Kjaer, M. and Ruyter, B.: Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids.
, Biochim Biophys Acta 1781,
326, 2008 | |
| Cells: | Preadipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Adipose/Fat: Gerbil | |
| |
| Nedergaard, J. and Lindberg, O.: The Brown Fat Cell
, Int Rev Cytol 74,
187, 1982 | |
| Cells: | Brown fat |
| Enzyme(s) | Collagenase Type 1: 0.10% |
| Medium | Bicarbonate buffer |
Adipose/Fat: Guinea-Pig | |
| |
| Nedergaard, J. and Lindberg, O.: The Brown Fat Cell
, Int Rev Cytol 74,
187, 1982 | |
| Cells: | Brown fat |
| Enzyme(s) | Collagenase Type 1: 0.10% |
| Medium | Bicarbonate buffer |
Adipose/Fat: Hamster | |
| |
| Nedergaard, J. and Lindberg, O.: The Brown Fat Cell
, Int Rev Cytol 74,
187, 1982 | |
| Cells: | Brown fat |
| Enzyme(s) | Collagenase Type 1: 0.10% |
| Medium | Bicarbonate buffer |
Adipose/Fat: Hamster | |
| |
| DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species
, Am J Physiol 221,
850, 1971 | |
| Cells: | White fat |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Kreb's Ringer bicarbonate buffer |
Adipose/Fat: Human | |
| |
| Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat.
, Curr Protoc Stem Cell Biol
48,
e68, 2019 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 4: 150-300 u/ml |
| Medium | RPMI-1640 |
Adipose/Fat: Human | |
| |
| Tsurumachi, N., Akita, D., Kano, K., Matsumoto, T., Toriumi, T., Kazama, T., Oki, Y., Saito-Tamura, Y., Tonogi, M., Shimizu, N. and Honda, M.: Effect of Collagenase Concentration on The Isolation of Small Adipocytes from Human Buccal Fat Pad.
, J Oral Sci ,
, 2018 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 2: 0.01-0.5% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Wahl, S., Drong, A., Lehne, B., Loh, M., Scott, K., Kunze, S. et al: Epigenome-wide Association Study of Body Mass Index, and the Adverse Outcomes of Adiposity
, Nature
541,
81, 2017 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Adipose/Fat: Human | |
| |
| Duscher, D., Maan, Z., Luan, A., Aitzetmuller, M., Brett, E., Atashroo, D., Whittam, A., Hu, M., Walmsley, G., Houschyar, K., Schilling, A., Machens, H., Gurtner, G., Longaker, M. and Wan, D.: Ultrasound-Assisted Liposuction Provides a Source for Functional Adipose-Derived Stromal Cells.
, Cytotherapy
19,
1491-1500, 2017 | |
| Cells: | Stromal |
| Enzyme(s) | Collagenase Type 2: 0.075% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Lin, Y., Marin-Argany, M., Dick, C., Redhage, K., Blancas-Mejia, L., Bulur, P., Butler, G., Deeds, M., Madden, B., Williams, A., Wall, J., Dietz, A. and Ramirez-Alvarado, M.: Mesenchymal Stromal Cells Protect Human Cardiomyocytes from Amyloid Fibril Damage
, Cytotherapy
19,
1426-1437, 2017 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Munir, H., Ward, L., Sheriff, L., Kemble, S., Nayar, S., Barone, F., Nash, G. and McGettrick, H.: Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner.
, Stem Cells 35,
1636-1646, 2017 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM/F-12 |
Adipose/Fat: Human | |
| |
| Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome.
, Stem Cell Res Ther 8,
69, 2017 | |
| Cells: | Adipose derived mesenchymal stem |
| Enzyme(s) | Collagenase animal free: 0.1% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Szoke, K., Reinisch, A., Ostrup, E., Reinholt, F. and Brinchmann, J.: Autologous Cell Sources in Therapeutic Vasculogenesis: In Vitro and In Vivo Comparison of Endothelial Colony-Forming Cells from Peripheral Blood and Endothelial Cells Isolated from Adipose Tissue.
, Cytotherapy
18,
242-52, 2016 | |
| Cells: | Adipose derived endothelial |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells
, Cytotherapy
18,
371-83, 2016 | |
| Cells: | Adipose stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F12 |
Adipose/Fat: Human | |
| |
| Hu, J., Cao, Y., Xie, Y., Wang, H., Fan, Z., Wang, J., Zhang, C., Wang, J., Wu, C. aand Wang, S.: Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice.
, Stem Cell Res Ther 7,
130, 2016 | |
| Cells: | Dental pulp stem |
| Enzyme(s) | Collagenase animal free: 0.1% |
| Medium | HBSS |
Adipose/Fat: Human | |
| |
| Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells.
, Cytotherapy
17,
359-68, 2015 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage.
, BMC Med Genomics
8,
41, 2015 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | HBSS |
Adipose/Fat: Human | |
| |
| Kinoshita, K., Kuno, S., Ishimine, H., Aoi, N., Mineda, K., Kato, H., Doi, K., Kanayama, K., Feng, J., Mashiko, T., Kurisaki, A. and Yoshimura, K.: Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers.
, Stem Cells Transl Med 4,
146, 2015 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts.
, Plast Reconstr Surg
136,
189e-199e, 2015 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Esteve, D., Boulet, N., Volat, F., Zakaroff-Girard, A., Ledoux, S., Coupaye, M., Decaunes, P., Belles, C., Gaits-Iacovoni, F., Iacovoni, J., Remaury, A., Castel, B., Ferrara, P., Heymes, C., Lafonta, M. Boloumie, J.: Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells.
, Stem Cells 33,
1277-91, 2015 | |
| Cells: | Adipose stroma-vascular |
| Enzyme(s) | Neutral Protease: 2.4 u/ml Collagenase: 250 u/ml |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells.
, Cytotherapy
16,
915, 2014 | |
| Cells: | Adipose derived mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | see reference |
Adipose/Fat: Human | |
| |
| Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities.
, Cytotherapy
16,
789, 2014 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 1: 0.15% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods.
, Cytotherapy
16,
1220-8, 2014 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells.
, Tiss Eng
19,
473-8, 2013 | |
| Cells: | Adipose stromal stem |
| Enzyme(s) | Collagenase animal free: 200 u/ml |
| Medium | DMEM/Hams F-12 |
Adipose/Fat: Human | |
| |
| Doi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis.
, J Tissue Eng Regen Med
7,
864, 2013 | |
| Cells: | Stromal vascular fraction |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Cervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance.
, Stem Cells Transl Med 1,
206-20, 2012 | |
| Cells: | Adipose-derived stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| James, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering.
, Stem Cells Transl Med 1,
673, 2012 | |
| Cells: | Perivascular stem |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery.
, Stem Cells Transl Med 1,
230-6, 2012 | |
| Cells: | Adipose derived stromal vascular |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I., Curcio, C., Floris, M., Fiaschetti, V., Floris, R. and Cervell, V.: A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Platelet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction.
, Stem Cells Transl Med 1,
341-51, 2012 | |
| Cells: | Stromal vascular, adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Hagman, D., Kuzma, J., Larson, I., Foster-Schubert, K., Kuan, L., Cignarella, A., Geamanu, E., Makar, K., Gottlieb, J. and Kratz, M.: Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing.
, J Immunol Methods 386,
50, 2012 | |
| Cells: | Stromovascular |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS with BSA |
Adipose/Fat: Human | |
| |
| Naaijkens, B., Niessen, H., Prins, H., Krijnen, P., Kokhuis, T., de Jong, N,, van Hinsbergh, V., Kamp, O., Helder, M., Musters, R., van Dijk, A. and Juffermans, L.: Human Platelet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications.
, Cell Tissue Res 348,
119, 2012 | |
| Cells: | Adipose derived stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Wu, I., Nahas, Z., Kimmerling, K., Rosson, G. and Elisseeff, J.: An Injectable Adipose Matrix for Soft-Tissue Reconstruction.
, Plast Reconstr Surg
129,
1247, 2012 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F-12 |
Adipose/Fat: Human | |
| |
| Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential
, Vasc Cell
3,
5, 2011 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase: 0.25% Deoxyribonuclease I: 0.002% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.
, Lipids Health Dis
10,
171, 2011 | |
| Cells: | Renal adipose derived cells |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Yang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells.
, J Biomed Sci
18,
59, 2011 | |
| Cells: | Adipose derived mesenchymal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | L-DMEM |
Adipose/Fat: Human | |
| |
| Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates.
, Methods Mol Biol 702,
17-27, 2011 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/Ham's F-12 |
Adipose/Fat: Human | |
| |
| Basu, S., Haghiac, M., Surace, P., Challier, J., Guerre-Millo, M., Singh, K., Waters, T., Minium, J., Presley, L., Catalano, P. and Hauguel-de Mouzon, S.: Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation.
, Obesity
19,
476, 2011 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | RPMI |
Adipose/Fat: Human | |
| |
| Peters, R., Wolf, M., van den Broek, M., Nuvolone, M., Dannenmann, S., Stieger, B., Rapold, R., Konrad, D., Rubin, A., Bertino, J., Aguzzi, A., Heikenwalder, M. and Knuth, A.: Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture.
, PLoS ONE
5,
e15689, 2010 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | KRB |
Adipose/Fat: Human | |
| |
| Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering.
, J Biomed Mater Res 92,
979, 2010 | |
| Cells: | Adipose tissue-derived stem |
| Enzyme(s) | Collagenase Type 2: 1.0% |
| Medium | DMEM/F12 |
Adipose/Fat: Human | |
| |
| Zimmerlin, L., Donnenberg, V., Pfeifer, M., Meyer, EM, Peault, B., Rubin, JP and Donnenberg, A.: Stromal Vascular Progenitors in Adult Human Adipose Tissue.
, Cytometry 77,
22-30, 2010 | |
| Cells: | Stromal vascular |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | HBSS |
Adipose/Fat: Human | |
| |
| Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes.
, Cytotherapy
12,
538, 2010 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/Hams F-12 |
Adipose/Fat: Human | |
| |
| Suga, H., Eto, H., Shigeura, T., Inoue, K., Aoi, N., Kato, H., Nishimura, S., Manabe, I., Gonda, K. and Yoshimura, K.: IFATS Collection: Fibroblast Growth Factor-2-Induced Hepatocyte Growth Factor Secretion by Adipose-Derived Stromal Cells Inhibits Postinjury Fibrogenesis Through a c-Jun N-Terminal Kinase-Dependent Mechanism.
, Stem Cells 27,
238, 2009 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase: 0.075% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Cai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function.
, Stem Cells 27,
230, 2009 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Tan, H., Ramirez, C., Miljkovic, N., Li, H., Rubin, J. and Marra, K.: Thermosensitive Injectable Hyaluronic Acid Hydrogel for Adipose Tissue Engineering.
, Biomaterials 30,
6844, 2009 | |
| Cells: | Adipose tissue-derived stem |
| Enzyme(s) | Collagenase Type 2: 1.0% |
| Medium | DMEM/F12 |
Adipose/Fat: Human | |
| |
| Tandon, N., Goh, B., Marsano, A., Chao, P., Montouri-Sorrentino, C., Gimble, J. and Vunjak-Novakovic, G.: Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation.
, Conf Proc IEEE Eng Med Biol Soc 2009,
6517-21, 2009 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Traktuev, D., Merfeld-Clauss, S., Li, J., Kolonin, M., Arap, W., Pasqualini, R., Johnstone, B., and March, K.: A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks
, Circ Res 102,
77, 2008 | |
| Cells: | Adipose derived stromal cells |
| Enzyme(s) | Collagenase Type 1: see reference |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Minana, M., Carbonell-Uberos, F., Mirabet, V., Marin, S. and Encabo, A.: IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue.
, Stem Cells 26,
2696, 2008 | |
| Cells: | Stromal |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Nie, J., Chang, B., Traktuev, D., Sun, J., March, K., Chan, L., Sage, H., Pasqualini, R., Arap, W. and Kolonin, M.: IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells.
, Stem Cells 26,
2735, 2008 | |
| Cells: | Stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% Neutral Protease: |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Pilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells.
, Regen Med
3,
705-15, 2008 | |
| Cells: | Stem |
| Enzyme(s) | Collagenase: 280 u/ml |
| Medium | D-PBS |
Adipose/Fat: Human | |
| |
| Bujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha: Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat
, J Endocrinol 192,
279-88, 2007 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Adipose/Fat: Human | |
| |
| Schaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies
, Stem Cells 25,
818-27, 2007 | |
| Cells: | Adipose derived stromal cells |
| Enzyme(s) | Collagenase Type 1: 0.15% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors.
, J Cell Physiol 212,
702-9, 2007 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F12 |
Adipose/Fat: Human | |
| |
| Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK
, J Lipid Res 47,
653-64, 2006 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Adipose/Fat: Human | |
| |
| Koellensperger, E., Von Heimburg, D., Markowicz, M. and Pallua, N.: Human Serum from Platelet-Poor Plasma for the Culture of Primary Human Preadipocytes.
, Stem Cells 24,
1218-25, 2006 | |
| Cells: | Preadipocytes |
| Enzyme(s) | Collagenase Type 1: 196 u/ml |
| Medium | M199 |
Adipose/Fat: Human | |
| |
| Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture
, Mol Biol Cell
16,
1131-41, 2005 | |
| Cells: | Adipocytes, stromal vascular |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | HBSS |
Adipose/Fat: Human | |
| |
| Rodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse
, J Exp Med 201,
1397-405, 2005 | |
| Cells: | Multipotent adipose derived stem |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress.
, J Biomech Eng 127,
1081, 2005 | |
| Cells: | Adult stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Seboek, D., Linscheid, P., Zulewski, H., Langer, I., Christ-Crain, M., Keller, U., and Muller, B.: Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation
, J Clin Endocrinol Metab 89(10),
4833, 2004 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM/F12 |
Adipose/Fat: Human | |
| |
| Quickler, M., Sinha, B., Tomlinson, J., Bujalska, I., Stewart, P., Arlt, W.,: Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5
, J Endocrinol 183,
331, 2004 | |
| Cells: | Preadipocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F-12 |
Adipose/Fat: Human | |
| |
| Fain JN, Madan AK, Hiler ML, Cheema P, and Bahouth SW.: Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans
, Endocrinology 145,
2273, 2004 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.13% |
| Medium | see reference |
Adipose/Fat: Human | |
| |
| Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives
, Circulation 109,
656-63, 2004 | |
| Cells: | Stromal vascular, adipocytes |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM/F12 |
Adipose/Fat: Human | |
| |
| Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells
, Circulation 110,
349-55, 2004 | |
| Cells: | Stromal vascular, adipocytes, stem |
| Enzyme(s) | Collagenase: 300 u/ml |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| Gesta, S., Lolmede, K., Daviaud, D., Berlan, M., Bouloumie, A., Lafontan, M., Valet, P., and Saulnier-Blache, J.: Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression
, Horm Metab Res 35,
158, 2003 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | DMEM |
Adipose/Fat: Human | |
| |
| Patwardhan, R., Tubbs, R., Leonard, R., Kelly, D., Killingsworth, C., Rollins, D., Smith, W., Ideker, R., and Oakes, W.: Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery
, Ped Neurosurg 36,
314, 2002 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: see reference |
| Medium | Saline |
Adipose/Fat: Human | |
| |
| McTernan, P., Anderson, L., Anwar, A., Eggo, M., Crocker, J., Barnett, A., Stewart, P., and Kumar, S.: Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences
, J Clin Endocrinol Metab 87,
1327, 2002 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F12 |
Adipose/Fat: Human | |
| |
| Zuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells
, Mol Biol Cell
13,
4279-95, 2002 | |
| Cells: | Processed lipoaspirate cells |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | PBS |
Adipose/Fat: Human | |
| |
| McTernan PG, Anwar A, Eggo MC, Barnett AH, Stewart PM, Kumar S: Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue
, Int J Obes Relat Metab Disord
24,
875-81, 2000 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Adipose/Fat: Human | |
| |
| Gottschling-Zelle, H., Birgel, M., Scriba, D., Blum, W., and Hauner, H.: Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1
, Eur J Endocrinol 141 (4),
436, 1999 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/Ham's F-12 |
Adipose/Fat: Human | |
| |
| Zhang, H., Kumar, S., Barnett, A., and Eggo, M.: Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake
, J Clin Endocrinol Metab 84,
2550, 1999 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F12 |
Adipose/Fat: Human | |
| |
| Anderson, O., Gliemann, J., and Gammeltoft: Receptor Binding and Biological Effect of Insulin in Human Adipocytes
, Diabetologia 13,
589, 1977 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | Kreb's Ringer bicarbonate buffer |
Adipose/Fat: Mouse | |
| |
| Jones, J., Rabhi, N., Orofino, J., Gamini, R., Perissi, V., Vernochet, C. and Farmer, S.: The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet.
, Sci Rep
10,
2380, 2020 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Adipose/Fat: Mouse | |
| |
| Bowles, A., Strong, A., Wise, R., Thomas, R., Gerstein, B., Dutreil, M., Hunter, R., Gimble, J. and Bunnell, B.: Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis.
, Stem Cells 35,
532-544, 2017 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Adipose/Fat: Mouse | |
| |
| Maria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis.
, Cytotherapy
18,
1129-45, 2016 | |
| Cells: | Adipose mesenchyal stromal |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Adipose/Fat: Mouse | |
| |
| Yao, W., Lay, Y., Kot, A., Liu, R., Zhang, H., Chen, H., Lam, K. and Lane, N.: Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference.
, Stem Cells 34,
2587-2600, 2016 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Mouse | |
| |
| Jang, H., Bhasin, S., Guarneri, T., Serra, C., Schneider, M., Lee, M., Guo, W., Fried, S., Pencina, K. and Jasuja, R.: The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life.
, Endocrinology 156,
3737, 2015 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Krebs |
Adipose/Fat: Mouse | |
| |
| Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts.
, Plast Reconstr Surg
136,
189e-199e, 2015 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Adipose/Fat: Mouse | |
| |
| Kondo, T, Toyoshima, Y., Ishii, Y and Kyuwa, S.: Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice.
, Exp Anim 62,
319, 2013 | |
| Cells: | Stromal vascular |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | RPMI-1640 |
Adipose/Fat: Mouse | |
| |
| Takahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair.
, Stem Cells Transl Med 1,
803, 2012 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Adipose/Fat: Mouse | |
| |
| Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells.
, Blood 115,
957, 2010 | |
| Cells: | Stem and progenitor |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | HBSS |
Adipose/Fat: Mouse | |
| |
| De Matteis, R., Zingaretti, M., Murano, I., Vitali, A., Frontini, A., Giannulis, I., Barbatelli, G., Marcucci, F., Bordicchia, M., Sarzani, R., Raviola, E. and Cinti, S.: In Vivo Physiological Transdifferentiation of Adult Adipose Cells.
, Stem Cells 27,
2761, 2009 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Adipose/Fat: Mouse | |
| |
| Wong, K., Szeto, F, Zhang, W., Ye, H., Kong, J., Zhang, Z., Sun, X. and Li, Y: Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins.
, Am J Physiol/Endo 296,
820, 2009 | |
| Cells: | White adipocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Adipose/Fat: Mouse | |
| |
| Thangarajah, H., Vial, I., Chang, E., El-Ftesi, S., Januszyk, M., Chang, E., Paterno, J., Neofytou, E., Longaker, M. and Gurtner, G.: IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia.
, Stem Cells 27,
266, 2009 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase Type 2: 0.075% |
| Medium | DMEM |
Adipose/Fat: Mouse | |
| |
| Cho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model.
, Stem Cells 27,
259-65, 2009 | |
| Cells: | Adipose tissue-derived stem |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | Modified Eagles |
Adipose/Fat: Mouse | |
| |
| Aoyagi T, Shimba S, Tezuka M: Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes, J Health Sci 51,
21, 2005 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.15% |
| Medium | DMEM/F12 |
Adipose/Fat: Mouse | |
| |
| Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T: Adipose-derived adult stromal cells heal critical-size mouse calvarial defects
, Nat Biotechnol
22,
560-7, 2004 | |
| Cells: | Adipose-derived stromal cells |
| Enzyme(s) | Collagenase Type 2: 0.075% |
| Medium | PBS |
Adipose/Fat: Mouse | |
| |
| Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives
, Circulation 109,
656-63, 2004 | |
| Cells: | Stromal vascular, adipocytes |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM/F12 |
Adipose/Fat: Mouse | |
| |
| Ruan, H., Zarnowski, MJ., Cushman, S., and Lodish, H.: Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes
, J Biol Chem 278,
47585, 2003 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase: see reference |
| Medium | DMEM |
Adipose/Fat: Mouse | |
| |
| Nadler, S., Stoehr, J., Rabaglia, M., Schueler, K., Birnbaum, M., and Attie, A.: Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice
, Am J Physiol/Endo 281,
E1249, 2001 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Krebs-Ringer Phosphate HEPES (KRPH) |
Adipose/Fat: Mouse | |
| |
| Launder, T., Gegen, N., Knedler, A., and Harbeck, R.: The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue
, J Immunol Methods 102,
45, 1987 | |
| Cells: | Vascular endothelial |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | PBS |
Adipose/Fat: Porcine | |
| |
| Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells.
, Cells Tissues Organs
188,
251, 2008 | |
| Cells: | Adipose mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Adipose/Fat: Porcine | |
| |
| Ramsay, T.G.: Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes
, J Anim Sci 81,
3008, 2003 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F12 |
Adipose/Fat: Porcine | |
| |
| Liang, W. and Mills, S.: Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues.
, J Anim Sci 80,
963-70, 2002 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.067% |
| Medium | Krebs-Ringer |
Adipose/Fat: Porcine | |
| |
| Ding, S., McNeel, R., and Mersmann, H.: Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo
, Comp Biochem Physiol B
123,
307, 1999 | |
| Cells: | Adipose |
| Enzyme(s) | Collagenase Type 1: 300 u/ml |
| Medium | HEPES |
Adipose/Fat: Porcine | |
| |
| Wang Y, Fried SK, Petersen RN, Schoknecht PA: Somatotropin regulates adipose tissue metabolism in neonatal swine
, J Nutr 129,
139-45, 1999 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | Krebs-Ringer bicarbonate albumin |
Adipose/Fat: Porcine | |
| |
| Suryawan, A., Swanson, L., and Hu, C.: Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture
, J Anim Sci 75,
105, 1997 | |
| Cells: | Adipose, Stromal-vascular |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F12 |
Adipose/Fat: Rat | |
| |
| Dayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells
, Cytotherapy
19,
937-946, 2017 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Adipose/Fat: Rat | |
| |
| Ohta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury.
, Cytotherapy
19,
839-848, 2017 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | DMEM |
Adipose/Fat: Rat | |
| |
| Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model.
, Cytotherapy
17,
543-59, 2015 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | DMEM |
Adipose/Fat: Rat | |
| |
| Veronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells.
, Cytotherapy
17,
215-23, 2015 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 2: 0.075% |
| Medium | DMEM |
Adipose/Fat: Rat | |
| |
| Ogasawara, J., Izawa, T., Sakurai, T., Shirato, K., Ishibashi, Y., Ohira, Y., Ishida, H., Ohno, H. and Kizaki, T.: Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes.
, Biochem Biophys Res Commun
464,
348-53, 2015 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | Krebs-Ringer |
Adipose/Fat: Rat | |
| |
| Leblanc, A., Nguyen, Q., Touroo, J., Aird, A., Chang, R., Ng, C., Hoying, J. and Williams, S.: Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct.
, Stem Cells Transl Med 2,
896-905, 2013 | |
| Cells: | Stromal vascular |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | PBS |
Adipose/Fat: Rat | |
| |
| Thompson, A., Nunez, M., Davidson, R., Horm, T., Schnittker, K., Hart, M., Suarez, A. and Tsao, T.: Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue.
, J Lipid Res 53,
2797, 2012 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 2: 0.33% |
| Medium | RPMI 1640 |
Adipose/Fat: Rat | |
| |
| Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.
, Lipids Health Dis
10,
171, 2011 | |
| Cells: | Renal adipose derived cells |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Adipose/Fat: Rat | |
| |
| Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose.
, J Biomed Biotechnol
2010,
104296, 2010 | |
| Cells: | Brown adipocytes |
| Enzyme(s) | Collagenase Type 4: 0.1% Neutral Protease: 0.1% Trypsin: 0.05% |
| Medium | PBS |
Adipose/Fat: Rat | |
| |
| Wei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats.
, Stem Cells 27,
478, 2009 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Adipose/Fat: Rat | |
| |
| Tomiyama, K., Murase, N., Stolz, D., Toyokawa, H., O'Donnell, D., Smith, D., Dudas, J., Rubin, J. and Marra, K.: Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue.
, Stem Cells 26,
330, 2008 | |
| Cells: | Adipose tissue-derived stem |
| Enzyme(s) | Collagenase Type 2: 0.075% |
| Medium | DMEM |
Adipose/Fat: Rat | |
| |
| Aoki, S., Toda, S., Sakemi, T., and Sugihara, H.: Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro
, Cell Struct Funct 28,
55, 2003 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Ham's F12 |
Adipose/Fat: Rat | |
| |
| Mora, S., Yang, C., Ryder, J., Boeglin, D and Pessin, J: The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency
, Endocrinology 142,
1999, 2001 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | KRHB |
Adipose/Fat: Rat | |
| |
| Omatsu-Kanbe, M., and Matsuura, H.: Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes
, J Physiol
521 (3),
601, 1999 | |
| Cells: | Brown adipocytes |
| Enzyme(s) | Deoxyribonuclease I: 0.5% |
| Medium | DMEM |
Adipose/Fat: Rat | |
| |
| Serrero, G: Primary Culture in Defined Medium of Adipocyte Precursors,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
11B:6.1, 1995 | |
| Cells: | Adipocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM /F-12 |
Adipose/Fat: Rat | |
| |
| Charron, M.J. and Kahn, B.B.: Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo
, J Biol Chem 265,
7994, 1990 | |
| Cells: | Adipose Epididymal fat pads |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | Kreb's-Ringer bicarbonate buffer modified |
Adipose/Fat: Rat | |
| |
| Gaben-Cogneville, A., Poussin, B., Chamblier, M., Forgue-Fafitte, M., and Rosselin, G.: Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture
, Biochim Biophys Acta 968,
231, 1988 | |
| Cells: | Preadipocytes |
| Enzyme(s) | Collagenase Type 3: 0.10% |
| Medium | Parker Medium 199 |
Adipose/Fat: Rat | |
| |
| Woodward, Julie A. and Saggerson, E.: Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline
, Biochem J
238,
395, 1986 | |
| Cells: | Brown adipocytes Interscapular & cervical depots |
| Enzyme(s) | Collagenase: 0.2% Soybean Trypsin Inhibitor: 0.3% |
| Medium | Krebs Ringer bicarbonate buffer |
Adipose/Fat: Rat | |
| |
| Pessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P.: ß-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes
, J Biol Chem 258,
7386, 1983 | |
| Cells: | Adipocytes Epididymal fat pads |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Krebs Ringer bicarbonate buffer |
Adipose/Fat: Rat | |
| |
| Green, A. and Newsholme, E.: Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites
, Biochem J
180,
365, 1979 | |
| Cells: | Adipocytes Epididymal-fat pads |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | Kreb's Ringer |
Adipose/Fat: Rat | |
| |
| Stiles, J.W., Francendese, A.A. and Masoro, E.J.: Influence of Age on Size and Number of Fat Cells in the Epididymal Depot
, Am J Physiol 229,
1561, 1975 | |
| Cells: | White fat |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | Kreb's Ringer bicarbonate buffer |
Adipose/Fat: Rat | |
| |
| DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species
, Am J Physiol 221,
850, 1971 | |
| Cells: | White fat |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Kreb's Ringer bicarbonate buffer |
Adipose/Fat: Rat | |
| |
| Fain, J., Reed, N., and Saperstein, R.: Isolation and Metabolism of Brown Fat Cells
, J Biol Chem 242,
1887, 1967 | |
| Cells: | Brown fat |
| Enzyme(s) | Collagenase Type 1: 0.10% |
| Medium | Bicarbonate buffer |
Adipose/Fat: Rat | |
| |
| Rodbell, M.: Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis
, J Biol Chem 239 (2),
375, 1964 | |
| Cells: | Fat |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | Albumin-bicarbonate buffer |
Adipose/Fat: Squirrel | |
| |
| McFarlane, S., Mathers, K. and Staples, J.: Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator.
, Am J Physiol Regul Integr Comp Physiol
312,
R434-R442, 2017 | |
| Cells: | Brown adipocytes |
| Enzyme(s) | Collagenase Type 2: 0.16% |
| Medium | Krebs-Ringer phosphate |
Adrenal: Bovine | |
| |
| Dominguez, N., Rodriguez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells.
, Methods Mol Biol 846,
223, 2012 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.1-0.2% Deoxyribonuclease I: 0.003-0.015% Hyaluronidase: 0.015% |
| Medium | DMEM/F12 |
Adrenal: Bovine | |
| |
| Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B: Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core
, Cell Transplant 15,
121-33, 2006 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.125% |
| Medium | Locke's solution |
Adrenal: Bovine | |
| |
| Hahm, S., Chen, Y., Vinson, C. and Eiden, L.: A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization.
, Mol Pharmacol 64,
1503, 2003 | |
| Cells: | Chromaffin cells |
| Enzyme(s) | Collagenase: 0.1% Deoxyribonuclease I: 30 u/ml |
| Medium | DMEM |
Adrenal: Bovine | |
| |
| Ortega, J., Sagen, J., and Pappas, G.: Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS
, Cell Transplant 1,
33, 1992 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Locke's solution |
Adrenal: Bovine | |
| |
| Zhu, J., Li, W., Toews, M., and Hexum, T.: Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process
, J Pharmacol Exp Ther 263 (3),
1479, 1992 | |
| Cells: | Chromaffin |
| Enzyme(s) | Deoxyribonuclease I: 30 u/mg |
| Medium | HEPES |
Adrenal: Bovine | |
| |
| Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons
, In Vitro Cell Dev Biol 26,
335, 1990 | |
| Cells: | Heart Adrenal chromaffin Paraneurons |
| Enzyme(s) | Trypsin: 0.06% |
| Medium | 25mM HEPES buffered Locke's solution, CMF |
Adrenal: Bovine | |
| |
| Dahmer, M., Hart, P., and Perlman, R.: Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells
, J Neurochem 54 (3),
931, 1990 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.25 % |
| Medium | DMEM |
Adrenal: Bovine | |
| |
| Higgins, L., and Berg, D.: Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells
, J Neurosci 7 (6),
1792, 1987 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.2% Deoxyribonuclease I: 0.015% |
| Medium | Locke's solution |
Adrenal: Bovine | |
| |
| Aunis, D., Rotllan, P., and Miras-Portugal, M.: Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture, Neurochem Int 7,
89, 1985 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Locke's solution, CMF |
Adrenal: Bovine | |
| |
| Almazan, G., Aunis, D., Garcia, A., Montiel, C., Nicolas, G., and Sanchez-Garcia, P.: Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells
, Br J Biomed Sci 81,
599, 1984 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.15% |
| Medium | Kreb's, CMF |
Adrenal: Bovine | |
| |
| Pollard, H., Pazoles, C., Creutz, C., Scott, J., Zinder, O., and Hotchkiss, A.: An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells
, J Biol Chem 259,
1114, 1984 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | (see reference) |
Adrenal: Bovine | |
| |
| Cena, V., Garcia, A., Montiel, C., and Sanchez-Garcia, P.: Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells
, Br J Pharmacol 81,
119, 1984 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | CF Kreb's |
Adrenal: Bovine | |
| |
| Waymire, J., Bennett, W., Boehme, R., Hankins, L., Gilmer-Waymire, K., and Haycock, J.: Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture
, J Neurosci Methods 7,
329, 1983 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.025% |
| Medium | HBSS, modified |
Adrenal: Bovine | |
| |
| Knight, D. and Baker, P.: Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells
, Q J Exp Physiol 68,
123, 1983 | |
| Cells: | Medulla |
| Enzyme(s) | Hyaluronidase: 0.2% |
| Medium | Saline w/BSA 0.5% |
Adrenal: Bovine | |
| |
| Greenberg, A. and Zinder, O.: alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells
, Cell Tissue Res 226,
655, 1982 | |
| Cells: | Medulla |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Krebs-Ringer bicarbonate buffer, CMF |
Adrenal: Bovine | |
| |
| Wilson, S.P., and Viveros, O.H.: Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium
, Exp Cell Res
133,
159, 1981 | |
| Cells: | Chromaffin |
| Enzyme(s) | Deoxyribonuclease I: 15 µg/ml |
| Medium | Medium A (see reference) |
Adrenal: Bovine | |
| |
| Baker, P., and Knight., D: Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells
, Phil Trans R Soc Lond 296,
83, 1981 | |
| Cells: | Medulla |
| Enzyme(s) | Protease: 0.2% |
| Medium | Saline |
Adrenal: Bovine | |
| |
| Trifaro, J.M., and Lee, R.W.: Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures
, Neuroscience 5,
1533, 1980 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Locke's solution, CMF |
Adrenal: Bovine | |
| |
| Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro
, Adv Biochem Psychopharmacol 255,
51, 1980 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.5% Collagenase: 0.5% |
| Medium | HBSS |
Adrenal: Bovine | |
| |
| Kumakura, K., Karoum, F., Guidotti, A., and Costa, E.: Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells
, Nature
283,
489, 1980 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.25% |
| Medium | F-12 medium |
Adrenal: Bovine | |
| |
| Kilpatrick, D., Ledbetter, F., Carson, K., Kirshner, A., Slepetis, R., and Kirshner, N.: Stability of Bovine Adrenal Medulla Cells in Culture
, J Neurochem 35 (3),
679, 1980 | |
| Cells: | Medulla |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Locke's solution, CF |
Adrenal: Bovine | |
| |
| Unsicker, K., and Griesser, G.: Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures
, Neuroscience 5,
1445, 1980 | |
| Cells: | Medulla |
| Enzyme(s) | Collagenase Type 1: 0.5% |
| Medium | HBSS |
Adrenal: Bovine | |
| |
| Aunis, D., Guerold, B., Bader, M-F., and Cieselski-Treska, J.: Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells
, Neuroscience 5,
2261, 1980 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | DMEM |
Adrenal: Bovine | |
| |
| Hersey, R., and DiStefano, V.: Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells
, J Pharmacol Exp Ther 209 (1),
147, 1979 | |
| Cells: | Medullary |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | HEPES |
Adrenal: Bovine | |
| |
| Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells
, Proc Natl Acad Sci U S A 76,
5217, 1979 | |
| Cells: | Foreskin |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Dulbecco's MEM w/10% calf serum |
Adrenal: Bovine | |
| |
| Fenwick, E., Fajdiga, P., Howe, N., and Livett, B.: Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell
, J Cell Biol
76,
12, 1978 | |
| Cells: | Medulla |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | Kreb's, CF |
Adrenal: Bovine | |
| |
| Brooks, J.C.: The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion
, Endocrinology 101,
1369, 1977 | |
| Cells: | Chromaffin |
| Enzyme(s) | Hyaluronidase: 0.2% |
| Medium | HEPES, CF |
Adrenal: Bovine | |
| |
| Schneider, A., Herz, R., and Rosenheck, K.: Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla
, Proc Natl Acad Sci U S A 74,
5036, 1977 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | Kreb's Ringer bicarbonate buffer, CF |
Adrenal: Guinea-Pig | |
| |
| Role, L.W., Leeman, S.E., and Perlman, R.L.: Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla
, Neurochem Int 6,
1813, 1981 | |
| Cells: | Chromaffin Medulla |
| Enzyme(s) | Collagenase: |
| Medium | BSS (see reference) |
Adrenal: Guinea-Pig | |
| |
| Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro
, Adv Biochem Psychopharmacol 255,
51, 1980 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.5% Collagenase: 0.5% |
| Medium | HBSS |
Adrenal: Guinea-Pig | |
| |
| Hochman, J., and Perlman, R.L.: Catecholamine Secretion by Isolated Adrenal Cells
, Biochim Biophys Acta 421,
168, 1976 | |
| Cells: | Adrenal Chromaffin |
| Enzyme(s) | Collagenase: 0.05%-0.20% |
| Medium | Kreb's-Ringer bicarb glucose buffer, CF |
Adrenal: Hamster | |
| |
| Liang, B.T., and Perlman, R.L.: Catecholamine Secretion by Hamster Adrenal Cells
, J Neurochem 32,
927, 1979 | |
| Cells: | Adrenal Chromaffin |
| Enzyme(s) | Hyaluronidase: 0.20% |
| Medium | Kreb's Ringer bicarbonate buffer |
Adrenal: Human | |
| |
| Santana, M., Chung, K., Vukicevic, V., Rosmaninho-Salgado, J., Kanczkowski, W., Cortez, V., Hackmann, K., Bastos, C., Mota, A., Schrock, E., Bornstein, S., Cavadas, C. and Ehrhart-Bornstein, M.: Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla.
, Stem Cells Transl Med 1,
783, 2012 | |
| Cells: | Adrenal medulla progenitor |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM/F-12 |
Adrenal: Human | |
| |
| Jeon, Y., Baek, W., Chung, S., Shin, N., Kim, H., and Lee, S.: Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model.
, Korean J Anesthesiol
60,
357, 2011 | |
| Cells: | Chromaffin cells |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Locke's solution |
Adrenal: Human | |
| |
| Caroccia, B., Fassina, A., Seccia, T., Recarti, C., Petrelli, L., Belloni, A., Pelizzo, M. and Rossi, G.: Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method.
, Endocrinology 151,
1375, 2010 | |
| Cells: | Adrenocortical |
| Enzyme(s) | Collagenase Type 1: 0.2% Deoxyribonuclease I: 0.01% |
| Medium | Krebs Ringer |
Adrenal: Human | |
| |
| Tischler, A., DeLellis, R., Bailes, B., Nunnemacher, G.,Carabba, V., and Wolfe, H.: Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells
, Lab Invest 43,
399, 1980 | |
| Cells: | Chromaffin |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Eagle's MEM |
Adrenal: Human | |
| |
| Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells
, Proc Natl Acad Sci U S A 76,
5217, 1979 | |
| Cells: | Foreskin |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Dulbecco's MEM w/10% calf serum |
Adrenal: Mouse | |
| |
| Dominguez, N., Rodriguez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells.
, Methods Mol Biol 846,
223, 2012 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.1-0.2% Deoxyribonuclease I: 0.003-0.015% Hyaluronidase: 0.015% |
| Medium | DMEM/F12 |
Adrenal: Mouse | |
| |
| Kolski-Andreaco, A., Cai, H., Currle, D., Chandy, K. and Chow, R.: Mouse Adrenal Chromaffin Cell Isolation.
, J Vis Exp
2,
129, 2007 | |
| Cells: | Chromaffin |
| Enzyme(s) | Papain: 40 u/ml |
| Medium | DMEM |
Adrenal: Mouse | |
| |
| Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang: The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells
, J Neurosci 25,
10546-55, 2005 | |
| Cells: | Chromafin cells |
| Enzyme(s) | Papain: 20-25 u/ml |
| Medium | DMEM |
Adrenal: Ovine | |
| |
| Valego, N. and Rose, J.: A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells.
, Reprod Sci
17,
477, 2010 | |
| Cells: | Adrenocortical |
| Enzyme(s) | Collagenase Type 1: 0.4% |
| Medium | DMEM/Ham's F12 |
Adrenal: Ovine | |
| |
| Valego, N., Su, Y., Carey, L., Young, S., Tatter, S., Wang, J. and Rose, J.: Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression.
, Am J Physiol Regul Integr Comp Physiol
289,
R410, 2005 | |
| Cells: | Adrenocortical |
| Enzyme(s) | Collagenase Type 1: 0.4% |
| Medium | DMEM/Ham's F12 |
Adrenal: Ovine | |
| |
| Keating, D., Rychkov, G., Adams, M., Holgert, H., McMillen, I.C. and Roberts, M.: Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels.
, J Physiol
555,
489, 2004 | |
| Cells: | Chromaffin cells |
| Enzyme(s) | Collagenase Type 2: 0.2% Deoxyribonuclease I: 100 u/ml |
| Medium | Locke's solution |
Adrenal: Ovine | |
| |
| Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J: The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep
, J Endocrinol 162,
215-25, 1999 | |
| Cells: | Anterior pituitary |
| Enzyme(s) | Trypsin: 2.5% Deoxyribonuclease I: 0.004% |
| Medium | DMEM |
Adrenal: Rat | |
| |
| Dominguez, N., Rodriguez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells.
, Methods Mol Biol 846,
223, 2012 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.1-0.2% Deoxyribonuclease I: 0.003-0.015% Hyaluronidase: 0.015% |
| Medium | DMEM/F12 |
Adrenal: Rat | |
| |
| Gilabert, J, Montalvo, G, and Artalejo A.: Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays, Nat Protoc
,
294, 2006 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase Type 1: 0.26% Deoxyribonuclease I: 0.015% Hyaluronidase: 0.015% |
| Medium | HBSS |
Adrenal: Rat | |
| |
| Gilabert, J: Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture,Cell Biology of the Chromaffin Cell,Borges, R. and Gandia, L., Instituto Teofilo Hernando,
, 2004 | |
| Cells: | Chromaffin cells |
| Enzyme(s) | Collagenase Type 1: 0.26% Deoxyribonuclease I: 0.015% Hyaluronidase: 0.015% |
| Medium | HBSS |
Adrenal: Rat | |
| |
| Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel: An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells
, Am J Physiol Regul Integr Comp Physiol
284,
R1631-5, 2003 | |
| Cells: | Zona fasciculata/reticularis |
| Enzyme(s) | Collagenase: 0.4% |
| Medium | Krebs-HEPES |
Adrenal: Rat | |
| |
| Zhang L, Castell A, Avila E, Drucker-ColÃn R, Escobar A: Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons
, J Neuropathol Exp Neurol 59,
170-4, 2000 | |
| Cells: | Chromaffin cells |
| Enzyme(s) | Collagenase Type 1: 0.025% Deoxyribonuclease I: 0.0015% |
| Medium | DMEM |
Adrenal: Rat | |
| |
| Sayed, S., Whitehouse, B., and Jones, P.: Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis
, J Endocrinol 154,
449, 1997 | |
| Cells: | ZG ZFR |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | Kreb's |
Adrenal: Rat | |
| |
| Roskelley, C.D. and Auersperg, N.: Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture
, In Vitro Cell Dev Biol 26,
493, 1990 | |
| Cells: | Adrenocortical |
| Enzyme(s) | Deoxyribonuclease I: 0.005% |
| Medium | BSS |
Adrenal: Rat | |
| |
| Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis
, In Vitro Cell Dev Biol 26,
24, 1990 | |
| Cells: | Leydig Adrenal |
| Enzyme(s) | Collagenase Type 2: 0.03% (adrenal) |
| Medium | Krebs Ringer bicarbonate buffer |
Adrenal: Rat | |
| |
| Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons
, In Vitro Cell Dev Biol 26,
335, 1990 | |
| Cells: | Heart Adrenal chromaffin Paraneurons |
| Enzyme(s) | Trypsin: 0.06% |
| Medium | 25mM HEPES buffered Locke's solution, CMF |
Adrenal: Rat | |
| |
| Payet, N., Deziel, Y., and Lehoux, J.-G.: Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture
, J Steroid Biochem 20,
449, 1984 | |
| Cells: | Glomerulosa |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | MEM-d-Val |
Adrenal: Rat | |
| |
| Leonard, R.K., Auersperg, N., and Parkes, C.O.: Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells
, In Vitro 19,
46, 1983 | |
| Cells: | Adrenocortical |
| Enzyme(s) | Deoxyribonuclease I: 0.005% |
| Medium | BSS |
Adrenal: Rat | |
| |
| Li, C.H., Ng, T.B., and Cheng, C.H.K.: Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells
, Int J Pept Protein Res 19,
361, 1982 | |
| Cells: | Decapular Capsular Glomerulosa |
| Enzyme(s) | Deoxyribonuclease I: 0.01% |
| Medium | Medium 199 |
Adrenal: Rat | |
| |
| Englert, D.F.: An Optical Study of Isolated Rat Adrenal Chromaffin Cells
, Exp Cell Res
125,
369, 1980 | |
| Cells: | Chromaffin |
| Enzyme(s) | Trypsin: 0.10% |
| Medium | Ham's F-12 w/HEPES |
Adrenal: Rat | |
| |
| Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro
, Adv Biochem Psychopharmacol 255,
51, 1980 | |
| Cells: | Chromaffin |
| Enzyme(s) | Collagenase: 0.5% Collagenase: 0.5% |
| Medium | HBSS |
Adrenal: Rat | |
| |
| Braley, L., Williams, G., and Bradwin, G.: The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells
, Endocrinology 106 (1),
50, 1980 | |
| Cells: | Glomerulosa |
| Enzyme(s) | Deoxyribonuclease I: 0.05% |
| Medium | Kreb's |
Adrenal: Rat | |
| |
| Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells
, Proc Natl Acad Sci U S A 76,
5217, 1979 | |
| Cells: | Foreskin |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Dulbecco's MEM w/10% calf serum |
Adrenal: Rat | |
| |
| Unsicker, K., Krisch, B., Otten, U., and Thoenen, H.: Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids
, Proc Natl Acad Sci U S A 75 (7),
3498, 1978 | |
| Cells: | Medullary |
| Enzyme(s) | Trypsin: 0.125% |
| Medium | HBSS |
Adrenal: Rat | |
| |
| Barofsky, A., Feinstein, M., and Halkerston, I.: Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands
, Exp Cell Res
79,
263, 1973 | |
| Cells: | Cortical |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Kreb's Ringer bicarbonate buffer |
Adrenal: Rat | |
| |
| Kloppenborg, P., Island, D., Liddle, G., Michelakis, A., and Nicholson, W.: A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism
, Endocrinology 82,
1053, 1968 | |
| Cells: | Adrenal |
| Enzyme(s) | Collagenase Type 1: 0.5% |
| Medium | Kreb's Ringer bicarbonate buffer |
Bone: Bovine | |
| |
| Buschmann, M., Gluzband, Y., Grodzinsky, A., and Hunziker, E.: Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture
, J Cell Sci
108,
1497, 1995 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.4% |
| Medium | DMEM |
Bone: Chicken | |
| |
| Gay, C., Lloyd, Q., and Gilman, V.: Characteristics and Culture of Osteoblasts Derived From Avian Long Bone
, In Vitro Cell Dev Biol 30A,
379, 1994 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Trypsin: 0.03% |
| Medium | DMEM |
Bone: Chicken | |
| |
| Rosselot, G., Reginato, A.M., and Leach, R.M.: Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes
, In Vitro Cell Dev Biol 28A,
235, 1992 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Ham's F12 |
Bone: Chicken | |
| |
| Schiltz, J.R., Mayne R., and Holtzer, H.: The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture, Differentiation
1,
97, 1973 | |
| Cells: | Vertebrae chondroblasts |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Simm's, CMF |
Bone: Human | |
| |
| Agarwal, S., Loder, S., Sorkin, M., Li, S., Shrestha, S., Zhao, B., Mishina, Y., James, A. and Levi, B.: Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.
, Stem Cells 34,
1692-701, 2016 | |
| Cells: | Bone-cartilage-stromal |
| Enzyme(s) | Collagenase Type 2: 0.75% |
| Medium | HBSS |
Bone: Human | |
| |
| Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts.
, Bonekey Rep
3,
585, 2014 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Collagenase Type 2: 0.2% Trypsin: 0.25%-1.0% |
| Medium | DMEM |
Bone: Human | |
| |
| Kode, A.,Manavalan1, J.,Mosialou1, I., Bhagat G., Rathinam, C, Luo, N et al.: Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts
, Nature
506,
240, 2014 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Collagenase Type 3: 0.2% |
| Medium | MEM |
Bone: Human | |
| |
| Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ: Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute
, Cells Tissues Organs
179,
158-69, 2005 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | Basal Medium |
Bone: Human | |
| |
| Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage
, J Bone Miner Res 14,
362, 1999 | |
| Cells: | Bone Cells, Osteoblasts |
| Enzyme(s) | Collagenase Type 2: 200-250 u/ml |
| Medium | DMEM |
Bone: Human | |
| |
| Meikle, M., Boyd, S., Hembry, R., Compston, J., Croucher, P., and Reynolds, J.: Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines
, J Cell Sci
103,
1093, 1992 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | DMEM |
Bone: Human | |
| |
| Fedarko, N.S., Termine, J.D., Young, M.F. and Robey, P.G.: Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro
, J Biol Chem 265,
12200, 1990 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Collagenase Type 4: 250 u/ml |
| Medium | DMEM |
Bone: Mouse | |
| |
| Mohrin, D., Koukos, G., Alabdulaaly, L., Paw, J., Hake, K., Kolumam, G., Bouxsein, M., Baron, R., Kutskova, Y., Freund, A., Liu, J., Zavala-Solorio, J., Bhargava, S., Maxwell, Trumble, J., Brito, A., Hu, D. and Brooks, D.: Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells.
,Aging Cell Vol. 20,, ,
e13313, 2021 | |
| Cells: | Bone and bone marrow stromal |
| Enzyme(s) | Stemxyme 1: 0.1% Neutral Protease: 0.1% |
| Medium | PBS |
Bone: Mouse | |
| |
| Baryawno, N., Przybylski, D., Kowalczyk, M., Kfoury, Y., Severe, N., Gustafsson, K., Kokkaliaris, K., Mercier, F., Tabaka, M., Hofree, M., Dionne, D., Papazian, A., Lee, D., Ashenberg, O. and Subramanian, A.: A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia.
, Cell
177,
1915-1932.e16, 2019 | |
| Cells: | Bone and bone marrow stroma |
| Enzyme(s) | Stemxyme 1: 0.1% Neutral Protease: 0.1% |
| Medium | Media 199 |
Bone: Mouse | |
| |
| Wang, W., Majihail, G., Lui, C. and Zhou, L.: Osteoblast Sorting and Intracellular Staining of CXCL12.
, Bio Protoc
8,
, 2018 | |
| Cells: | Osteoblast |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | HBSS |
Bone: Mouse | |
| |
| Agarwal, S., Loder, S., Sorkin, M., Li, S., Shrestha, S., Zhao, B., Mishina, Y., James, A. and Levi, B.: Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.
, Stem Cells 34,
1692-701, 2016 | |
| Cells: | Bone-cartilage-stromal |
| Enzyme(s) | Collagenase Type 2: 0.75% |
| Medium | HBSS |
Bone: Mouse | |
| |
| Igarashi, H., Akahoshi, N., Ohto-Nakanishi, T., Yasuda, D. and Ishii, S.: The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells.
, Sci Rep
5,
11410, 2015 | |
| Cells: | Bone stromal |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Bone: Mouse | |
| |
| Lee, D., Choung, H., Kim, H., Gronostajski, R., Yang, Y., Ryoo, H., Lee, Z., Kim, H., Cho, E. and Park, J.: NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression.
, Stem Cells 32,
2467-79, 2014 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Collagenase Type 1: 1.0% Neutral Protease: 1.6% |
| Medium | MEM |
Bone: Mouse | |
| |
| Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts.
, Bonekey Rep
3,
585, 2014 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Collagenase Type 2: 0.2% Trypsin: 0.25%-1.0% |
| Medium | DMEM |
Bone: Mouse | |
| |
| Kode, A.,Manavalan1, J.,Mosialou1, I., Bhagat G., Rathinam, C, Luo, N et al.: Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts
, Nature
506,
240, 2014 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Collagenase Type 3: 0.2% |
| Medium | MEM |
Bone: Mouse | |
| |
| Stern, A., Stern, M., Van Dyke, M., Jahn, K., Prideaux, M. and Bonewald, L.: Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice.
, Biotechniques
52,
361, 2012 | |
| Cells: | Osteocytes |
| Enzyme(s) | Collagenase Type 1: 300 u/ml |
| Medium | MEM |
Bone: Mouse | |
| |
| Nakamura, Y., Arai, F., Iwasaki, H., Hosokawa, K., Kobayashi, I., Gomei, Y., Matsumoto, Y., Yoshihara, H. and Suda, T.: Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells.
, Blood 116,
1422, 2010 | |
| Cells: | Endosteal cells |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Bone: Mouse | |
| |
| Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H. and Matsuzaki, Y.: Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow.
, J Exp Med 206,
2483-96, 2009 | |
| Cells: | Bone marrow |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM |
Bone: Mouse | |
| |
| Sakai, E., Miyamoto, H., Okamoto, K., Kato, Y., Yamamoto, K., and Sakai, H.: Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages
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130,
823, 2001 | |
| Cells: | Osteoclasts |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | DMEM |
Bone: Mouse | |
| |
| Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage
, J Bone Miner Res 14,
362, 1999 | |
| Cells: | Bone Cells, Osteoblasts |
| Enzyme(s) | Collagenase Type 2: 200-250 u/ml |
| Medium | DMEM |
Bone: Mouse | |
| |
| Takanashi, H., Matsuishi, T., and Yoshizato, K.: Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells
, In Vitro Cell Dev Biol 30A,
384, 1994 | |
| Cells: | Osteoblast-like Cells Stromal Cell Lines Hematopoietic Blast Cells |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Eagle's MEM |
Bone: Mouse | |
| |
| Chen,T. and Feldman,D.: Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells
, J Biol Chem 256,
5561, 1981 | |
| Cells: | Neonatal bone |
| Enzyme(s) | Collagenase Type 2: 0.20% |
| Medium | Tris-buffered saline |
Bone: Rat | |
| |
| Jeon, J., Lee, M. and Yang, H.: Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation.
, Biomater Res
22,
4, 2018 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Collagenase Type 2: 0.1% Trypsin: 0.25% |
| Medium | DMEM |
Bone: Rat | |
| |
| Jacobs, F., Gijsen, H., Van de Vyver, M. and Ferris, W.: Vanadate Impedes Adipogenesis in Mesenchymal Stem Cells Derived from Different Depots within Bone.
, Front Endocrinol
7,
108, 2016 | |
| Cells: | Proximal femur stem |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | HBSS |
Bone: Rat | |
| |
| Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts.
, Bonekey Rep
3,
585, 2014 | |
| Cells: | Osteoblasts |
| Enzyme(s) | Collagenase Type 2: 0.2% Trypsin: 0.25%-1.0% |
| Medium | DMEM |
Bone: Rat | |
| |
| Owen, T., Aronow, M., Shalhoub, V., Barone, L., Wilming, L., Tassinari, M., Kennedy, M., Pockwinse, S., Lian, J., and Stein, G.: Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix
, J Cell Physiol 143,
420, 1990 | |
| Cells: | Calvaria |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | MEM |
Bone: Rat | |
| |
| Ernst, M., and Froesch, E.: Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I
, Calcif Tissue Int 40,
27, 1987 | |
| Cells: | Osteoblastlike cells |
| Enzyme(s) | Collagenase Type 2: 0.3% |
| Medium | MEM |
Bone: Rat | |
| |
| Peck, W., Birge, S., and Fedak, S.: Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation
, Science 146,
1476, 1964 | |
| Cells: | Calvaria |
| Enzyme(s) | Collagenase: 0.01%-0.6% |
| Medium | Tris-buffered saline |
Brain: Bovine | |
| |
| Kanda, T., Yoshino, H., Ariga, T., Yamawaki, M., and Yu, R.: Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy
, J Cell Biol
126 (1),
235, 1994 | |
| Cells: | Microvascular endothelial |
| Enzyme(s) | Neutral Protease: 0.005% |
| Medium | Medium 199 |
Brain: Bovine | |
| |
| Wolburg H, Neuhaus J, Kniesel U, Krauss B, Schmid EM, Ocalan M, Farrell C, Risau W: Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes
, J Cell Sci
107,
1347, 1994 | |
| Cells: | Brain endothelial cells |
| Enzyme(s) | Collagenase Type 2: 0.35% |
| Medium | DMEM |
Brain: Bovine | |
| |
| Miller, D., Audus, K., and Borchardt, R.: Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier, J Tiss Cul Meth 14,
217, 1992 | |
| Cells: | Endothelial |
| Enzyme(s) | Neutral Protease: 0.125% |
| Medium | MEM |
Brain: Bovine | |
| |
| Machi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries
, In Vitro Cell Dev Biol 26,
291, 1990 | |
| Cells: | Cerebral artery Endothelial |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | HBSS |
Brain: Bovine | |
| |
| Estrada, C., Bready, J., Berliner, J., and Cancilla, P.: Choline Uptake by Cerebral Capillary Endothelial Cells in Culture
, J Neurochem 54,
1467, 1990 | |
| Cells: | Capillary endothelial |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | DMEM |
Brain: Bovine | |
| |
| Audus, K., and Borchardt, R.: Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism, Pharm Res 3 (2),
81, 1986 | |
| Cells: | Endothelial |
| Enzyme(s) | Neutral Protease: 0.5% |
| Medium | MEM |
Brain: Bovine | |
| |
| Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain
, In Vitro Cell Dev Biol 21,
172, 1985 | |
| Cells: | Endothelial Brain arteries |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | Dulbecco's PBS |
Brain: Bovine | |
| |
| Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain
, J Biol Chem 253,
1592, 1978 | |
| Cells: | Oligodendroglia Neural |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | (see reference) |
Brain: Guinea-Pig | |
| |
| Kay, A.R., and Wong, R.K.S.: Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems
, J Neurosci Methods 16,
227, 1986 | |
| Cells: | Neurons |
| Enzyme(s) | Trypsin: 0.06-0.08% |
| Medium | PIPES saline |
Brain: Human | |
| |
| Mizee, M., Miedema, S., van der Poel, M., Adelia , S., van Strien, M., Melief, J., Smolders, J., Hendrickx, D., Heutinck, K., Hamann, J. and Huitinga, I.: Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables.
, Acta Neuropathol
5,
16, 2017 | |
| Cells: | Microglia |
| Enzyme(s) | Collagenase Type 1: 300 u/ml Trypsin: 0.125% |
| Medium | DMEM |
Brain: Human | |
| |
| Spaethling, J., Na, Y., Lee, J., Ulyanova, A., Baltuch, G., Bell, T., Brem, S., Chen, H., Dueck, H., Fisher, S., Garcia, M., Khaladkar, M., Kung, D., Lucas, T., O'Rourke, D. and Stefanik, D.: Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics.
, Cell Rep
18,
791-803, 2017 | |
| Cells: | Neuronal |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | Neurobasal |
Brain: Human | |
| |
| Zhang, Y., Sloan, S., Clarke, L., Caneda, C., Plaza, C., Blumenthal, P., Vogel, H., Steinberg, G., Edwards, M., Li, G., Duncan, J., Cheshier, S., Shuer, L., Chang, E., Grant, G., Gephart, M. and Barres, B.: Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.
, Neuron 89,
37-53, 2016 | |
| Cells: | Astrocytes and neurons |
| Enzyme(s) | Papain: 7.5-20 u/ml |
| Medium | RPMI |
Brain: Human | |
| |
| Rustenhoven, J., Park Thomas, I., Schweder, P., Scotter, J., Correia, J., Smith, A., Gibbons, H., Oldfield, R., Bergin, P., Mee, E., Faull, R., Curtis, M., Scott, G. and Dragunow, M.: Isolation of Highly Enriched Primary Human Microglia for Functional Studies.
, Sci Rep
6,
19371, 2016 | |
| Cells: | Microglia |
| Enzyme(s) | Neutral Protease: 10 u/ml Papain: 2.5 u/ml |
| Medium | Hibernate A |
Brain: Human | |
| |
| Volovitz, I, Shapira, N., Ezer, H., Gafni, A., Lustgarten, M., Alter, T., Ben-Horin, I., Barzilai, O., Shahar, T., Kanner, A., Fried, I., Veshchev, I., Grossman, R. and Ram, Z,: A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells.
, BMC Neurosci
17,
30, 2016 | |
| Cells: | Brain tumor |
| Enzyme(s) | Neutral Protease: 0.11 u/ml Collagenase Type 4: 0.05% Hyaluronidase: 1,000 u/ml Deoxyribonuclease I: 5 u/ml |
| Medium | HBSS |
Brain: Human | |
| |
| Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges
, Methods Mol Biol 1345,
299, 2016 | |
| Cells: | Amyloid Fibrils |
| Enzyme(s) | Collagenase Type 3: 0.02% Deoxyribonuclease I: 0.001% |
| Medium | 50mM Tris |
Brain: Human | |
| Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.: Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion.
, Neuro Oncol
13,
70-83, 2011 | |
| Cells: | Brain tumor |
| Enzyme(s) | Collagenase Type 1: 0.04% Hyaluronidase: 0.01% Deoxyribonuclease I: 0.02% Neutral Protease: 0.008% |
| Medium | DMEM/F12 |
Brain: Human | |
| |
| Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.: Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.
,Neuro Oncol Vol. 11,, ,
109, 2009 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 4: 0.1% Hyaluronidase: 0.07% Deoxyribonuclease I: 0.04% |
| Medium | see reference |
Brain: Human | |
| |
| Klegeris Andis, McGeer PatrickL: Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity
, Glia 51,
56-64, 2005 | |
| Cells: | Microglia |
| Enzyme(s) | Trypsin: 0.25% Deoxyribonuclease I: .005% |
| Medium | DMEM/F12 |
Brain: Human | |
| |
| Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels
, Brain Res Bull 21,
785, 1988 | |
| Cells: | Microvessels |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | DMEM |
Brain: Human | |
| |
| Roher, A.E., Palmer, K.C., Chau, V., and Ball, M.J.: Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein
, J Cell Biol
107,
2703, 1988 | |
| Cells: | Neuronal |
| Enzyme(s) | Deoxyribonuclease I: 10 µg/ml |
| Medium | Tris-HCl, 50 mM, CaCl2, 2 mM |
Brain: Human | |
| |
| Vinters, H.V., Reave, S., Costello, P., Girvin, J.P., and Moore, S.A.: Isolation and Culture of Cells Derived From Human Cerebral Microvessels
, Cell Tissue Res 249,
657, 1987 | |
| Cells: | Microvessels |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Serum-free modified Lewis medium |
Brain: Insect | |
| |
| Hadzic, T., Park, D., Abruzzi, K., Yang, L., Trigg, J., Rohs, R., Rosbash, M. and Taghert, P.: Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED.
, Nucleic Acids Res
43,
2199-215, 2015 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 50 u/ml |
| Medium | Modified Dissecting Saline |
Brain: Insect | |
| |
| Gu, H. and O'Dowd, D.: Whole Cell Recordings from Brain of Adult Drosophila.
, J Vis Exp
6,
248, 2007 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | Saline |
Brain: Insect | |
| |
| Sicaeros, B. Campusano, J. and O'Dowd, D.: Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae.
, J Vis Exp
4,
200, 2007 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 4 u/ml |
| Medium | DMEM |
Brain: Insect | |
| |
| Gu, H. and O'Dowd, D.: Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ.
, J Neurosci 26,
265-72, 2006 | |
| Cells: | Kenyon |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | Recording saline |
Brain: Monkey | |
| |
| Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS: Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection
, J Immunol 167,
5429-38, 2001 | |
| Cells: | Brain cells |
| Enzyme(s) | Collagenase Type 2: 500 u/ml Deoxyribonuclease I: 28 u/ml |
| Medium | HBSS |
Brain: Mouse | |
| |
| Calvo, Belen, Rubio, F., Fernandez, M. and Tranque, P.: Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry.
,IBRO Rep Vol. 8,, ,
36-47, 2020 | |
| Cells: | Microglia, astrocytes, lymphocytes |
| Enzyme(s) | Collagenase Type 2: 120 u/ml Papain: 20 u/ml Neutral Protease: 1.2 u/ml Deoxyribonuclease I: 20 u/ml |
| Medium | EBSS |
Brain: Mouse | |
| |
| Kramer, D., Risso, D., Kosillo, ., Ngai, J. and Bateup, H.: Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability
,eNeuro Vol. 5,, ,
1, 2018 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 10 u/ml Deoxyribonuclease I: 10 u/ml |
| Medium | Artificial CSF |
Brain: Mouse | |
| |
| Swartzlander, D., Propson, N., Roy, E., Saito, T., Saido, T., Wang, B. and Zheng, H.: Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease.
, JCI Insight
3,
, 2018 | |
| Cells: | Astrocytes, microglia, endothelia, oligodendrocytes |
| Enzyme(s) | Papain: 20 u/ml Collagenase Type 1: 0.01% |
| Medium | HBSS |
Brain: Mouse | |
| |
| Zhang, Y., Sloan, S., Clarke, L., Caneda, C., Plaza, C., Blumenthal, P., Vogel, H., Steinberg, G., Edwards, M., Li, G., Duncan, J., Cheshier, S., Shuer, L., Chang, E., Grant, G., Gephart, M. and Barres, B.: Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.
, Neuron 89,
37-53, 2016 | |
| Cells: | Astrocytes and neurons |
| Enzyme(s) | Papain: 7.5-20 u/ml |
| Medium | RPMI |
Brain: Mouse | |
| |
| Baruch, K., Rosenzweig, N., Kertser, A., Deczkowska, A., Sharif, A., Spinrad, A., Tsitsou-Kampeli, A., Sarel, A., Cahalon, L. and Schwartz, M.: Breaking Immune Tolerance by Targeting Foxp3(+) Regulatory T Cells Mitigates Alzheimer's Disease Pathology.
, Nat Commun
6,
7967, 2015 | |
| Cells: | Chloroid plexus |
| Enzyme(s) | Collagenase Type 4: 400 u/ml |
| Medium | PBS |
Brain: Mouse | |
| |
| Luo,Y.,Coskun, V., Liang, A.,Yu, J., Cheng, L.,Ge, W., Shi, Z., Zhang, K., Li, C., Lin, H., Luo, D., Wang, J., Lin, C., Dai, Z., Zhu, H., Zhang, J., Liu, J., Liu, H.,deVellis, J., Horvath, S., Sun, Y. and Li, S.: Different Patterns of Molecular Forms of Somatostatin are Released by the Rat Median Eminence and Hypothalamus.
, Cell
161,
1175, 2015 | |
| Cells: | Neural |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | PBS |
Brain: Mouse | |
| |
| Ryg-Cornejo, V., Ioannidis, L. and Hansen, D.: Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice.
, J Vis Exp
71,
e50112, 2013 | |
| Cells: | Brain-sequestered leukocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% Deoxyribonuclease I: 2 u/ml |
| Medium | RPMI |
Brain: Mouse | |
| |
| Shah. G., Price, T., Banks, W., Morofuji, Y., Kovac, A., Ercal, N., Sorenson, C., Shin, E., Sheibani, N.: Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis.
, J Pharmacol Exp Ther 344,
637-45, 2013 | |
| Cells: | Cerebral pericytes |
| Enzyme(s) | Collagenase Type 2: 0.1% Deoxyribonuclease I: 30 u/ml |
| Medium | DMEM |
Brain: Mouse | |
| |
| Haseleu, J., Anlauf, E., Blaess, S., Endl, E. and Derouiche, A.: Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs).
, Front Cell Neurosci
7,
54, 2013 | |
| Cells: | Glial |
| Enzyme(s) | Papain: 20 u/ml Deoxyribonuclease I: 0.0005% |
| Medium | EBSS |
Brain: Mouse | |
| |
| Foo, L.: Purification of Rat and Mouse Astrocytes by Immunopanning.
, Cold Spring Harb. Protoc. 2013,
421-32, 2013 | |
| Cells: | Astrocytes |
| Enzyme(s) | Papain: 5 u/ml Deoxyribonuclease I: 0.004% |
| Medium | DPBS |
Brain: Mouse | |
| |
| Saxena, A., Wagatsuma, A., Noro, Y., Kuji, T., Asaka-Oba, A., Watahiki, A., Gurnot, C., Fagiolini, M., Hensch, T. and Carninci, P.: Trehalose-Enhanced Isolation of Neuronal Sub-Types from Adult Mouse Brain.
, Biotechniques
52,
381, 2012 | |
| Cells: | Neurons |
| Enzyme(s) | PDS kit: with modifications |
| Medium | DMEM/F12 |
Brain: Mouse | |
| |
| O'Meara, R., Ryan, S., Colognato, H. and Kothary, R.: Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues.
, J Vis Exp
54,
3324, 2011 | |
| Cells: | Oligodendrocytes, dorsal root ganglia |
| Enzyme(s) | Papain: 0.15% Deoxyribonuclease I: 0.006% |
| Medium | DMEM |
Brain: Mouse | |
| |
| Chung, W., Farley, J., Swenson, A., Barnard, J., Hamilton, G., Chiposi, R. and Drummond, H.: Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells.
, Am J Physiol Cell Physiol
298,
C1198, 2010 | |
| Cells: | Vascular smooth muscle cells |
| Enzyme(s) | Papain: 0.05% Collagenase Type 4: 0.15% Elastase: 0.05% |
| Medium | PBS |
Brain: Mouse | |
| |
| Lee, H., Greene, L., Mason, C. and Manzini, M.: Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons.
, J Vis Exp
23,
990, 2009 | |
| Cells: | Neurons |
| Enzyme(s) | PDS kit: with modifications |
| Medium | HBSS |
Brain: Mouse | |
| |
| Sher, F., Rossler, R., Brouwer, N., Balasubramaniyan, V., Boddeke, E. and Copray, S.: Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2.
, Stem Cells 26,
2875, 2008 | |
| Cells: | Astrocytes |
| Enzyme(s) | Trypsin: 0.25% Deoxyribonuclease I: 1,000 u/ml |
| Medium | HBSS |
Brain: Mouse | |
| |
| Fasano, C., Thibault, D. and Trudeau, L.: Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer
,Current Protocols in Neuroscience Vol. 44,, ,
3.21.1, 2008 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | Neurobasal |
Brain: Mouse | |
| |
| Hilgenberg, L. and Smith, M.: Preparation of Dissociated Mouse Cortical Neuron Cultures.
, J Vis Exp
10,
562, 2007 | |
| Cells: | Cortical neurons |
| Enzyme(s) | Papain: 4-10 u/ml |
| Medium | Neurobasal |
Brain: Mouse | |
| |
| Klein C, Butt SJ, Machold RP, Johnson JE, and Fishell G.: Cerebellum- and forebrain-derived stem cells possess intrinsic regional character
, Development
132,
4497, 2005 | |
| Cells: | Neurosperes |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM/F12 |
Brain: Mouse | |
| |
| Seaberg, R., Smukler, S. and Van der Kooy, D.: Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain.
, Dev Biol 278,
71, 2005 | |
| Cells: | Neural progenitor |
| Enzyme(s) | PDS kit: per instructions |
| Medium | see reference |
Brain: Mouse | |
| |
| Oliver, T., Read, T., Kessler, J., Mehmeti, A., Wells, J., Huynh, T., Lin, S. and Wechsler-Reya, R.: Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma.
, Development
132,
2425, 2005 | |
| Cells: | Granule cell precursors, pre-neoplastic and tumor cells |
| Enzyme(s) | Papain: 10 u/ml Deoxyribonuclease I: 250 u/ml |
| Medium | Neurobasal/B27 |
Brain: Mouse | |
| |
| Hernandez, F., Perez, M., Lucas, J., Mata, A., Bhat, R. and Avila, J.: Glycogen Synthase Kinase-3 plays a Crucial Role in Tau Exon 10 Splicing and Intranuclear Distribution of SC35. Implications for Alzheimer's Disease.
, J Biol Chem 279,
3801, 2004 | |
| Cells: | Cortical neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal |
Brain: Mouse | |
| |
| Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H: Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia
, J Biol Chem 277,
4816, 2002 | |
| Cells: | Microglia |
| Enzyme(s) | Papain: 90 u/ml Deoxyribonuclease I: 2000 u/ml |
| Medium | Eagle's MEM |
Brain: Mouse | |
| |
| O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL: IGF-I and microglia/macrophage proliferation in the ischemic mouse brain
, Glia 39,
85, 2002 | |
| Cells: | Microglia |
| Enzyme(s) | Trypsin: 0.125% Collagenase Type 2: 0.01% Deoxyribonuclease I: .005% |
| Medium | RPMI-1640 |
Brain: Mouse | |
| |
| Smeyne Michelle, Smeyne RichardJ: Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease
, Brain Res Brain Res Protoc 9,
105-11, 2002 | |
| Cells: | Postnal substantia nigra |
| Enzyme(s) | PDS kit: per instructions |
| Medium | see reference |
Brain: Mouse | |
| |
| Estivill-Torrus, G., Pearson, H., Van Heyningen, V., Price, D. and Rashbass, P.: Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors.
, Development
129,
455, 2002 | |
| Cells: | Cortical progenitors |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Serum free medium |
Brain: Mouse | |
| |
| Martin-Aparicio, E., Yamamoto, A., Hernandez, F., Hen, R., Avila, J. and Lucas, J.: Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease.
, J Neurosci 21,
8772, 2001 | |
| Cells: | Neurons and glia |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal |
Brain: Mouse | |
| |
| Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S, and Ishizaki Y.: A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation
, J Neurosci 20,
5756, 2000 | |
| Cells: | Cerebellar granule cell precursors |
| Enzyme(s) | Papain: 0.435% Deoxyribonuclease I: 0.05% |
| Medium | EBSS |
Brain: Mouse | |
| |
| Lim, D, and Alvarez-Buylla, A.: Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis
, Proc Natl Acad Sci U S A 96,
7526, 1999 | |
| Cells: | Astrocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Brain: Mouse | |
| |
| Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS: Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice
, Learn Mem
5,
317-30, 1998 | |
| Cells: | Hippocampal cells |
| Enzyme(s) | Papain: 10 u/ml |
| Medium | DMKM |
Brain: Mouse | |
| |
| Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.: A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells
, Brain Res 503,
326, 1989 | |
| Cells: | Papillae, taste receptor |
| Enzyme(s) | Pronase E: 0.15% |
| Medium | Carbonate-Phosphate buffer (see reference) |
Brain: Mouse | |
| |
| Shrier, B., Wilson, S., and Nirenberg, M.: Cultured Cell Systems and Methods for Neurobiology
, Vol. 32,, ,
765, 1974 | |
| Cells: | Neural |
| Enzyme(s) | Trypsin NF 1:250: 0.25% |
| Medium | BSS |
Brain: Ovine | |
| |
| Reddy, R., Amodei, R., Estill, C., Stormshak, F., Meaker, M. and Roselli, C.: Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture.
, PLoS ONE
10,
e0129521, 2015 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 0.2% Deoxyribonuclease I: 0.1% |
| Medium | Hibernate |
Brain: Ovine | |
| |
| Lepore G, Gadau S, Mura A, Zedda M, Farina V: Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury.
, Eur J Histochem 53,
e28, 2009 | |
| Cells: | Neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Brain: Ovine | |
| |
| Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain
, J Biol Chem 253,
1592, 1978 | |
| Cells: | Oligodendroglia Neural |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | (see reference) |
Brain: Porcine | |
| |
| Robinson, D.H., Kang,Y., Deschner, S.H., and Nielsen, T.B.: Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture
, In Vitro Cell Dev Biol 26,
169, 1990 | |
| Cells: | Microvascular |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HBSS |
Brain: Rat | |
| |
| Kurachi, M., Mikuni, M. and Ishizaki, Y.: Extracellular Vesicles from Vascular Endothelial Cells Promote Survival, Proliferation and Motility of Oligodendrocyte Precursor Cells.
, PLoS ONE
11,
e0159158, 2016 | |
| Cells: | Oligodendrocyte precursor cells |
| Enzyme(s) | Papain: 16.5 u/ml |
| Medium | HBSS |
Brain: Rat | |
| |
| Folts, C., Scott-Hewitt, N., Proschel, C., Mayer-Proschel, M. and Noble, M.: Lysosomal Re-acidification Prevents Lysosphingolipid-Induced Lysosomal Impairment and Cellular Toxicity.
, PLoS Biol
14,
e1002583, 2016 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | HBSS |
Brain: Rat | |
| |
| Foo, L.: Purification of Rat and Mouse Astrocytes by Immunopanning.
, Cold Spring Harb. Protoc. 2013,
421-32, 2013 | |
| Cells: | Astrocytes |
| Enzyme(s) | Papain: 5 u/ml Deoxyribonuclease I: 0.004% |
| Medium | DPBS |
Brain: Rat | |
| |
| Kaiser, O., Aliuos, P., Wissel, K., Lenarz, T., Werner, D., Reuter, G., Kral, A. and Warnecke, A.: Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi After Digestion with Papain.
, PLoS ONE
8,
e80490, 2013 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | Neuro medium |
Brain: Rat | |
| |
| Cao, R., Li, A., Cho, H., Lee, B. and Obrietan, K.: Mammalian Target of Rapamycin Signaling Modulates Photic Entrainment of the Suprachiasmatic Circadian Clock.
, J Neurosci 30,
6302, 2010 | |
| Cells: | Suprachiasmatic nucleus neurons |
| Enzyme(s) | Papain: 100 u/ml |
| Medium | MEM |
Brain: Rat | |
| |
| Chen, N., Newcomb, J., Garbuzova-Davis, S., Davis Sanberg, C., Sanberg, P. and Willing, A.: Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro.
, Aging Dis 1,
173, 2010 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate A |
Brain: Rat | |
| |
| Tanaka, S., Shaikh, I., Chiocca, E. and Saeki, Y.: The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development.
, PLoS ONE
4,
e5922, 2009 | |
| Cells: | Cerebellar granule neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | PBS |
Brain: Rat | |
| |
| Shigetomi, E. and Khakh, B.: Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes.
, J Vis Exp
26,
1142, 2009 | |
| Cells: | Astrocytes |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | EBSS |
Brain: Rat | |
| |
| Richler Esther, Chaumont Severine, Shigetomi Eiji, Sagasti Alvaro, Khakh Baljit S: Tracking transmitter-gated P2X cation channel activation in vitro and in vivo
, Nat Methods
5,
87-93, 2008 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 10 u/ml |
| Medium | EBSS |
Brain: Rat | |
| |
| Akanda, N., Tofighi, R., Brask, J., Tamm, C., Elinder, F. and Ceccatelli, S.: Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells.
, Cell Cycle 7,
3225, 2008 | |
| Cells: | Hippocampal |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Neurobasal |
Brain: Rat | |
| |
| Peptan, I., Hong, L. and Evans, C.: Multiple Differentiation Potentials of Neonatal Dura Mater-Derived Cells.
, Neurosurgery
60,
346, 2007 | |
| Cells: | Dura mater |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | DMEM |
Brain: Rat | |
| |
| Jekabsons MB, Nicholls DG: Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation
, Cell Death Differ
13,
1595-610, 2006 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate |
Brain: Rat | |
| |
| Velasco Myrian, Garcia Esperanza, Onetti Carlos G: Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons
, Cell Mol Neurobiol 26,
307-19, 2006 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Brain: Rat | |
| |
| Khakh Baljit S, Fisher James A, Nashmi Raad, Bowser David N, Lester Henry A: An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy
, J Neurosci 25,
6911-20, 2005 | |
| Cells: | Hippocampal neuron |
| Enzyme(s) | Papain: 10 u/ml |
| Medium | MEM |
Brain: Rat | |
| |
| Mizoguchi Y, Kanematsu T, Hirata M, Nabekura J: A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex
, J Biol Chem 278,
44097, 2003 | |
| Cells: | Visual cortical |
| Enzyme(s) | PDS kit: per instructions |
| Medium | EBSS |
Brain: Rat | |
| |
| Floris S, Van den Born J, van der Pol SM, Dijkstra CD, De Vries HE: Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium
, J Neuropathol Exp Neurol 62,
780, 2003 | |
| Cells: | Cerebral endothelial |
| Enzyme(s) | Collagenase Type 3: 0.2% |
| Medium | MEM |
Brain: Rat | |
| |
| Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I and Barker, J: GABA(B) receptors mediate motility signals for migrating embryonic cortical cells
, Cereb Cortex 11,
744-53, 2001 | |
| Cells: | Cortical cells |
| Enzyme(s) | Papain: 20 u/ml Deoxyribonuclease I: .005% |
| Medium | EBSS |
Brain: Rat | |
| |
| Brzezinska AK, Gebremedhin D, Chilian WM, Kalyanaraman B, Elliott SJ.: Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells
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H1883, 2000 | |
| Cells: | Cerebral artery smooth muscle cells |
| Enzyme(s) | Papain: 1.5 mg/ml Collagenase Type 4: 1.5 mg/ml |
| Medium | Physiological Salt Solution |
Brain: Rat | |
| |
| Varney MA, Cosford ND, Jachec C, Rao SP, Sacaan A, Lin FF, Bleicher L, Santori EM, Flor PJ, Allgeier H, Gasparini F, Kuhn R, Hess SD, Velicelebi G, Johnson EC: SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5
, J Pharmacol Exp Ther 290,
170, 1999 | |
| Cells: | Cortical neurons |
| Enzyme(s) | PDS kit: with modifications |
| Medium | Neurobasal Medium |
Brain: Rat | |
| |
| Boehm S, Betz H: Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors
, J Neurosci 17,
4066, 1997 | |
| Cells: | Hippocampal cells |
| Enzyme(s) | Papain: 1 mg/ml |
| Medium | DMEM |
Brain: Rat | |
| |
| Brewer, G.J.: Isolation and culture of adult rat hippocampal neurons of any age
, J Neurosci Methods 71,
143, 1997 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | HibernateA/B27 |
Brain: Rat | |
| |
| Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE: Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis
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2648-53, 1997 | |
| Cells: | Hippocampal and cortical neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal media |
Brain: Rat | |
| |
| Pixley, S.K.: The Olfactory Nerve Contains Two Populations of Glia
, In Vitro 5,
269, 1992 | |
| Cells: | Glial |
| Enzyme(s) | Trypsin: 0.0625% |
| Medium | MEM, sterile |
Brain: Rat | |
| |
| Holzwarth, J., Glaum, S., and Miller, R.: Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes
, Glia 5,
239, 1992 | |
| Cells: | Astrocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM, HBSS |
Brain: Rat | |
| |
| Acheson, A., Barker, P., Alderson, R., Miller, F., and Murphy, R.: Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF
, Neuron 7,
265, 1991 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | HEPES buffered DMEM |
Brain: Rat | |
| |
| Doron, D., Jacobowitz, D., Heldman, E., Feurerstein, G., Pollard, H., and Hallenbeck, J.: Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels
, In Vitro Cell Dev Biol 27A,
689, 1991 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 2: 0.5% |
| Medium | Medium 199 |
Brain: Rat | |
| |
| Mattson, M., and Kater, S.: Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions
, Brain Res 490,
110, 1989 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Trypsin: 0.2% Deoxyribonuclease I: 10 ug/ml |
| Medium | HBSS |
Brain: Rat | |
| |
| Matsuda, M.: Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture
, In Vitro Cell Dev Biol 24 (10),
1031, 1988 | |
| Cells: | Fetal rat brain |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | DMEM |
Brain: Rat | |
| |
| Giulian, D. and Baker, T.J.: Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain
, J Neurosci 6,
2163, 1986 | |
| Cells: | Cerebral cortices |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS |
Brain: Rat | |
| |
| Goldman, J.E., Geier, S.S., and Hirano, M.: Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture
, J Neurosci 6,
52, 1986 | |
| Cells: | Germinal matrix |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS |
Brain: Rat | |
| |
| Bartlett, W. and Banker, G.: An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts
, J Neurosci 4,
1944, 1984 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS, CMF |
Brain: Rat | |
| |
| Rothman, S.: Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death
, J Neurosci 4 (7),
1884, 1984 | |
| Cells: | Hippocampi |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS |
Brain: Rat | |
| |
| Ahmed, Z., Walker, P., and Fellows, R.: Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture
, J Neurosci 3 (12),
2448, 1983 | |
| Cells: | Neural |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Brain: Rat | |
| |
| Peterfreund, R. and Vale, W.: High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell
, Brain Res 239,
463, 1982 | |
| Cells: | Cerebral cortex Hypothalmus |
| Enzyme(s) | Deoxyribonuclease I: 0.001% |
| Medium | HEPES |
Brain: Rat | |
| |
| Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.: Primary Culture of Rat Cerebral Microvascular Endothelial Cells
, Lab Invest 46,
554, 1982 | |
| Cells: | Endothelial Cerebral |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | HBSS |
Brain: Rat | |
| |
| Swaiman, K., Neale, E., Fitzgerald, S., and Nelson, P.: A Method for Large-scale Production of Mouse Brain Cortical Cultures
, Brain Res 255,
361, 1982 | |
| Cells: | Cortical |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Puck's D1 (see reference) |
Brain: Rat | |
| |
| Abney, E., Bartlett, P., and Raff, M.: Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain
, Dev Biol 83,
301, 1981 | |
| Cells: | Glial |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Eagle's MEM/DMEM |
Brain: Rat | |
| |
| Bowman, P., Betz, A., Ar, D., Wolinsky, J., Penney, J., Shivers, R., and Goldstein, G.: Primary Culture of Capillary Endothelium From Rat Brain
, In Vitro 17 (4),
353, 1981 | |
| Cells: | Capillary endothelium Pericytes |
| Enzyme(s) | Neutral Protease: 0.1% |
| Medium | Medium 199 |
Brain: Rat | |
| |
| Williams, S., Gillis, J., Matthews, M., Wagner, R., and Bitensky, M.: Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity
, J Neurochem 35 (2),
374, 1980 | |
| Cells: | Microvessels Endothelial |
| Enzyme(s) | Collagenase Type 2: 0.75% |
| Medium | Ringers-HEPES buffer |
Brain: Rat | |
| |
| Phillips, P., Kumar, P., Kumar, S., and Waghe, M.: Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter
, J Anat 129,
261, 1979 | |
| Cells: | Endothelial |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | BSS |
Brain: Rat | |
| |
| Banker, G., and Cowan, M.: Rat Hippocampal Neurons in Dispersed Cell Culture
, Brain Res 126,
397, 1977 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS |
Brain: Rat | |
| |
| Rowe, V., Neale, E., Avins, L., Guroff, G., and Schrier, B.: Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons
, Exp Cell Res
104,
345, 1977 | |
| Cells: | Pineal |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM, MEM |
Brain: Shellfish | |
| |
| Hadley, R.D., Bodnar, D.A., and Kater, S.B.: Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation
, J Neurosci 5,
3145, 1985 | |
| Cells: | Neurons Buccal ganglia |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | Saline, Sterile |
Cartilage: Bovine | |
| |
| White, R. and Gibson, J.: The Effect of Oxygen Tension on Calcium Homeostasis in Bovine Articular Chondrocytes.
, J Orthop Surg Res
5,
27, 2010 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Cartilage: Bovine | |
| |
| Hwang, Y., Sangaj, N. and Varghese, S.: Interconnected Macroporous Poly(ethylene glycol) Cryogels as a Cell Scaffold for Cartilage Tissue Engineering.
, Tissue Eng Part A
16,
3033-41, 2010 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Cartilage: Bovine | |
| |
| Lima, E., Tan, A., Tai, T., Marra, K., DeFail, A., Ateshian, G. and Hung, C.: Genipin Enhances the Mechanical Properties of Tissue-Engineered Cartilage and Protects Against Inflammatory Degradation When Used as a Medium Supplement.
, J Biomed Mater Res 91,
692, 2009 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 4: 390 u/ml |
| Medium | DMEM |
Cartilage: Bovine | |
| |
| Mackintosh, D., and Mason, R.: Pharmacological Actions of 17 Beta-oestradiol on Articular Cartilage Chondrocytes and Chondrosarcoma Chondrocytes in the Absence of Oestrogen Receptors
, Biochim Biophys Acta 964,
295, 1988 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Trypsin: 0.20% |
| Medium | HBSS |
Cartilage: Bovine | |
| |
| Klagsbrun, M.: Large Scale Preparation of Chondrocytes
,Methods of Enzymology Vol. 58,, ,
560, 1979 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.20% |
| Medium | PBS |
Cartilage: Canine | |
| |
| Lee, J., Kim, H., Kim, J., Bae, S., Joo, D., Huh, K., Fang, Y., Jeong, J., Kim, M. and Kim, Y.: Microencapsulation of Pancreatic Islets with Canine Ear Cartilage for Immunoisolation.
, Transplant Proc 44,
1091-4, 2012 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.3% Trypsin: 0.25% |
| Medium | Ham's F12 |
Cartilage: Chicken | |
| |
| Wong, M., and Tuan, R.: Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture
, In Vitro Cell Dev Biol 29A,
917, 1993 | |
| Cells: | Mesenchymal |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | DMEM (see reference) |
Cartilage: Chicken | |
| |
| Genge, B., Wu, L. and Wuthier, R.: Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins
, J Biol Chem 265,
4703, 1990 | |
| Cells: | Matrix vesicles Epiphyseal growth plate |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Tris-buffered saline |
Cartilage: Chicken | |
| |
| Genge, B.R., Wu, L.N.Y., and Wuthier, R.E.: Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles
, J Biol Chem 264,
10917, 1989 | |
| Cells: | Matrix vesicles |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | (see reference) |
Cartilage: Chicken | |
| |
| Gionti, E., Capasso, O., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro
, J Biol Chem 258 (11),
7190, 1983 | |
| Cells: | Fibroblasts Epithelial-like |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | E 199 medium |
Cartilage: Chicken | |
| |
| Capasso, O., Gionti, E., Pontarelli, G., Ambesi-Impiobato, F., Nitsch, L., Tajana, G., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro
, Exp Cell Res
142,
197, 1982 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Trypsin: 0.75% |
| Medium | Coon's modified F-12 |
Cartilage: Chicken | |
| |
| Ahrens, P., Solursh, M., and Reiter, R.: Stage-Related Capacity for Limb Chondrogenesis in Cell Culture
, Dev Biol 60,
69, 1977 | |
| Cells: | Wing buds |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Saline G |
Cartilage: Equine | |
| |
| Visser, J., Levett, P., te Moller, N., Besems, J., Boere, K., van Rijen, M., de Grauw, J., Dhert, W., van Weeren, P. and Malda, J.: Crosslinkable Hydrogels Derived from Cartilage, Meniscus, and Tendon Tissue.
, Tissue Eng Part A
21,
1195-206, 2015 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | DMEM |
Cartilage: Equine | |
| |
| Ley, C., Svala, E., Nilton, A., Lindahl, A., Eloranta, M., Ekman, S. and Skioldebrand, E.: Effects of High Mobility Group Box Protein-1, Interleukin-1B, and Interleukin-6 on Cartilage Matrix Metabolism in Three-Dimensional Equine Chondrocyte Cultures.
, Conn Tissue Res 52,
290-300, 2011 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.08% |
| Medium | DMEM/F12 |
Cartilage: Goat | |
| |
| Bekkers,J, Creemers, L, Tsuchida, A, van Rijen, M, Custers, R, Dhert, W and Saris, D.: One-Stage Focal Cartilage Defect Treatment with Bone Marrow Mononuclear Cells and Chondrocytes Leads to Better Macroscopic Cartilage Regeneration Compared to Microfracture in Goats.
, Osteoarthritis Cartilage
21,
950-6, 2013 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 2% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Muraglia, A., Nguyen, V., Nardini, M., Mogni, M., Coviello, D., Dozin, B., Strada, P., Baldelli, I., Formica, M., Cancedda, R. and Mastrogiacomo, M.: Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support.
, Front Bioeng Biotechnol
5,
66, 2017 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Trypsin: 0.25% Hyaluronidase: 0.1% Collagenase Type 2: 1000 u/ml Collagenase Type 1: 400 u/ml |
| Medium | Ham's F12 |
Cartilage: Human | |
| |
| Andrews, S., Kunze, M., Mulet-Sierra, A., Williams, L., Ansari, K., Osswald, M. and Adesida, A.: Strategies to Mitigate Variability in Engineering Human Nasal Cartilage.
, Sci Rep
7,
6490, 2017 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Capsoni, F., Ongari, A., Lonati, C., Accetta, R., Gatti, S. and Catania, A.: Alpha-Melanocyte-Stimulating-Hormone (a-MSH) Modulates Human Chondrocyte Activation Induced by Proinflammatory Cytokines.
, BMC Musculoskelet Disord
16,
154, 2015 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Tamai, M., Nagasao, T., Yanaga, H., Hamamoto, Y., Kogure, T. and Tanaka, Y.: Correction of Secondary Deformity after Nuss Procedure for Pectus Excavatum by Means of Cultured autologous Cartilage Cell Injection.
, Int J Surg Case Rep
15,
70-3, 2015 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Pallu, S., Francin, P., Guillaume, C., Gegout-Pottie, P., Netter, P., Mainard, D., Terlain, B. and Presle, N.: Obesity Affects the Chondrocyte Responsiveness to Leptin in Patients with Osteoarthritis.
, Arthritis Res Ther 12,
R112, 2010 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% Pronase: 0.15% |
| Medium | DMEM/F12 |
Cartilage: Human | |
| |
| Kim Wan-Uk, Kwok Seung-Ki, Hong Kyung-Hee, Yoo Seung-Ah, Kong Jin-Sun, Choe Jongseon, Cho Chul-Soo: Soluble Fas ligand inhibits angiogenesis in rheumatoid arthritis
, Arthritis Res Ther 9,
R42, 2007 | |
| Cells: | Synoviocytes |
| Enzyme(s) | Collagenase Type 1: 0.4% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Marsano A, Millward-Sadler SJ, Salter DM, Adesida A, Hardingham T, Tognana E, Kon E, Chiari-Grisar C, Nehrer S, Jakob M, Martin I: Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes
, Osteoarthritis Cartilage
15,
48-58, 2007 | |
| Cells: | Meniscus and cartilage |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Tallheden T, Bengtsson C, Brantsing C, Sjogren-Jansson E, Carlsson L, Peterson L, Brittberg M, and Lindahl A.: Proliferation and Differentiation Potential of Chondrocytes from Osteoarthritic Patients
, Arthritis Res Ther 7(3),
R560, 2005 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.08% |
| Medium | DMEM/F12 |
Cartilage: Human | |
| |
| Liagre B, Vergne-Salle P, Corbiere C, Charissoux JL, and Beneytout JL.: Diosgenin, a Plant Steroid, Induces Apoptosis in Human Rheumatoid Arthritis Synoviocytes with Cyclooxygenase-2 Overexpression
, Arthritis Res Ther 6(4),
R373, 2004 | |
| Cells: | Synovial |
| Enzyme(s) | Collagenase: 0.15% Hyaluronidase: 0.1% Deoxyribonuclease I: 0.015% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Jakob M, Demarteau O, Schafer D, Stumm M, Heberer M, and Martin I.: Enzymatic Digestion of Adult Human Articular Cartilage Yields a Small Fraction of the Total Available Cells
, Conn Tissue Res 44,
173, 2003 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | DMEM |
Cartilage: Human | |
| |
| McEvoy A., Murphy E., Ponnio T., Conneely O., Bresnihan B., FitzGerald O., and Murphy E.: Activation of Nuclear Orphan Receptor NURR1 Transcription by NF-kappa B and Cyclic Adenosine 5'-Monophosphate Response Element-binding Protein in Rheumatoid Arthritis Synovial Tissue
, J Immunol 168(6),
2979, 2002 | |
| Cells: | Synoviocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | RPMI |
Cartilage: Human | |
| |
| Rotter N, Bonassar LJ, Tobias G, Lebl M, Roy AK, Vacanti CA: Age dependence of cellular properties of human septal cartilage: implications for tissue engineering
, Arch Otolaryngol Head Neck Surg 127,
1248-52, 2001 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Sarkissian M, Lafyatis R: Integrin engagement regulates proliferation and collagenase expression of rheumatoid synovial fibroblasts
, J Immunol 162,
1772-9, 1999 | |
| Cells: | Synovial fibroblasts |
| Enzyme(s) | Collagenase: 0.4% |
| Medium | DMEM |
Cartilage: Human | |
| |
| Dunham B P, Koch R J: Basic fibroblast growth factor and insulinlike growth factor I support the growth of human septal chondrocytes in a serum-free environment
, Arch Otolaryngol Head Neck Surg 124,
1325-30, 1998 | |
| Cells: | Septal chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% Hyaluronidase: 0.01% Deoxyribonuclease I: 0.015% |
| Medium | DMEM/F12 |
Cartilage: Human | |
| |
| Dayer, J., Krane, S., Russell, R., and Robinson, D.: Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells
, Proc Natl Acad Sci U S A 73 (3),
945, 1976 | |
| Cells: | Synovial tissue |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM (see reference) |
Cartilage: Human | |
| |
| Srivastava, V.M.L., Malemud, C.J., Hough, A.J., Bland, J.H., and Sokoloff, L.: Preliminary Experience with Cell Culture of Human Articular Chondrocytes
, Arthritis Rheum 17,
165, 1974 | |
| Cells: | Articular chondrocytes |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | BSS |
Cartilage: Human | |
| |
| Manning, W.K., and Bonner, W.M.: Isolation and Culture of Chondrocytes From Human Adult Articular Cartilage
, Arthritis Rheum 10,
235, 1967 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase: |
| Medium | GBSS |
Cartilage: Mouse | |
| |
| Otsuru, S., Hofmann, T., Raman, P., Olson, T., Guess, A., Dominici, M. and Horwitz, E.: Genomic and Functional Comparison of Mesenchymal Stromal Cells Prepared Using Two Isolation Methods.
, Cytotherapy
17,
262-70, 2015 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 86.5 u/ml |
| Medium | DMEM |
Cartilage: Mouse | |
| |
| Terpstra, L, Prud'homme, J, Arabian, A, Takeda, S, Karsenty, G, Dedhar, S, and St-Arnaud, R.: Reduced Chondrocyte Proliferation and Chondrodysplasia in Mice Lacking the Integrin-linked Kinase in Chondrocytes
, J Cell Biol
162,
139, 2003 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM |
Cartilage: Ovine | |
| |
| Kojima Koji, Bonassar Lawrence J, Roy Amit K, Mizuno Hirokazu, Cortiella Joaquin, Vacanti Charles A: A Composite Tissue-Engineered Trachea Using Sheep Nasal Chondrocyte and Epithelial Cells
, FASEB J
17,
823-8, 2003 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.3% |
| Medium | Ham's F-12 |
Cartilage: Porcine | |
| |
| Chowdhury, T., Schulz, R., Rai, S., Thuemmler, C., Wuestneck, N., Bader, A and Homandberg, G: Biomechanical Modulation of Collagen Fragment-Induced Anabolic and Catabolic Activities in Chondrocyte/Agarose Constructs.
, Arthritis Res Ther 12,
R82, 2010 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DEMEM |
Cartilage: Porcine | |
| |
| Graff RD, Lazarowski ER, Banes AJ, Lee GM: ATP release by mechanically loaded porcine chondrons in pellet culture
, Arthritis Rheum 43,
1571-9, 2000 | |
| Cells: | Chondrons |
| Enzyme(s) | Neutral Protease: 0.3% Collagenase: 0.2% |
| Medium | PBS |
Cartilage: Rabbit | |
| |
| Ju, X., Deng, M., Ao, Y., Yu, C., Wang, J., Yu, J., Cui, G. and Hu, Y.: Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis.
, Yakugaku Zasshi
130,
1053-60, 2010 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.025% Pronase: 0.2% |
| Medium | DMEM |
Cartilage: Rabbit | |
| |
| Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.: Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion
, Ann Rheum Dis 56,
108, 1997 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Hyaluronidase: .05% Collagenase Type 2: 0.2% Trypsin: 0.2% |
| Medium | Gey's solution |
Cartilage: Rabbit | |
| |
| Plaas, A., Sandy, J., and Kimura, J.: Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes
, J Biol Chem 263,
7560, 1988 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Protease XIV: 5 mg/g of tissue |
| Medium | Ham's F-12 |
Cartilage: Rabbit | |
| |
| Benya, P.D., Padilla, S.R., and Nimni, M.E.: The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II
, Biochemistry
16,
865, 1977 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | Gey's BSS |
Cartilage: Rabbit | |
| |
| Schindler, F.H., Ose, M.A., and Solursh, M.: The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture
, In Vitro 12,
44, 1976 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Saline G, CMF |
Cartilage: Rabbit | |
| |
| Green, J.R., and William, T.: Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting, Clin Orthop Relat Res
,
237, 1976 | |
| Cells: | Articular chondrocytes Hyaline |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | Gey's BSS |
Cartilage: Rabbit | |
| |
| Bentley, G., and Greer , R.: Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits
, Nature
230,
385, 1971 | |
| Cells: | Epiphyseal Articular cartilage |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Eagle's basal medium |
Cartilage: Rat | |
| |
| Mei, L., Shen, B., Ling, P., Liu, S., Xue, J., Liu, F., Shao, H., Chen, J., Ma, A. and Liu, X.: Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model.
, PLoS ONE
12,
e0176107, 2017 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Collagenase Type 2: 0.15% Trypsin: 0.25% |
| Medium | DMEM/F12 |
Cartilage: Rat | |
| |
| Shimomura, Y., Yoneda, T., and Suzuki, F.: Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib
, Calcif Tissue Res 19,
179, 1975 | |
| Cells: | Chondrocytes |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | Ham's F-12 medium |
Colon: Guinea-Pig | |
| |
| Raghupathi, R., Duffield, M., Zelkas, L., Meedeniya, A., Brookes, S., Sia, T., Wattchow, D., Spencer, N. and Keating, D.: Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells.
, J Physiol
591,
5959-75, 2013 | |
| Cells: | Enterochromaffin |
| Enzyme(s) | Trypsin: 0.05% Collagenase Type 1: 0.1% |
| Medium | DMEM |
Colon: Guinea-Pig | |
| |
| Kang, M., Maguma, H., Smith, T., Ross, G., Dewey, W. and Akbarali, H.: The Role of β-arrestin2 in the Mechanism of Morphine Tolerance in the Mouse and Guinea Pig Gastrointestinal Tract.
, J Pharmacol Exp Ther 340,
567-76, 2012 | |
| Cells: | Myenteric ganglia |
| Enzyme(s) | Collagenase Type 4: 0.2% Protease: 0.1% |
| Medium | Kreb's solution |
Colon: Human | |
| |
| Moser, A., Spindelboeck, W., Strohmaier, H., Enzinger, C., Gattringer, T., Fuchs, S., Fazekas, F., Gorkiewicz, G., Wurm, P., Hogenauer, C. and Khalil, M.: Mucosal Biopsy Shows Immunologic Changes of the Colon in Patients with Early MS
, Neurol Neuroimmunol Neuroinflamm
4,
e362, 2017 | |
| Cells: | Lamina propria mononuclear |
| Enzyme(s) | Collagenase Type 1: 0.1% Deoxyribonuclease I: 5 u/ml |
| Medium | HBSS |
Colon: Human | |
| |
| Rollo, B., Zhang, D., Stamp, L., Menheniott, T., Stathopoulos, L., Denham, M., Dottori, M., King, S., Hutson, J. and Newgreen, D.: Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon, Cell Mol Gastroenterol Hepatol 2,
92, 2016 | |
| Cells: | Colon enteric neural |
| Enzyme(s) | Neutral Protease: 0.5% Collagenase animal free: 0.1% Hyaluronidase: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | Ham's F-12 |
Colon: Human | |
| |
| VanDussen, K., Marinshaw, J., Shaikh, N., Miyoshi, H., Moon, C., Tarr, P., Ciorba, M. and Stappenbeck, T.: Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays.
, Gut 64,
911-20, 2015 | |
| Cells: | Gastrointestinal epithelial |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F12 |
Colon: Human | |
| |
| Ali, M., Anand, S., Tangella, K., Ramkumar, D. and Saif, T.: Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry.
, J Vis Exp
,
e52532, 2015 | |
| Cells: | Colon |
| Enzyme(s) | Trypsin: 0.1% Collagenase: 0.1% |
| Medium | HBSS |
Colon: Human | |
| |
| Raghupathi, R., Duffield, M., Zelkas, L., Meedeniya, A., Brookes, S., Sia, T., Wattchow, D., Spencer, N. and Keating, D.: Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells.
, J Physiol
591,
5959-75, 2013 | |
| Cells: | Enterochromaffin |
| Enzyme(s) | Trypsin: 0.05% Collagenase Type 1: 0.1% |
| Medium | DMEM |
Colon: Human | |
| |
| Roig, A., Eskiocak, U., Hight, S., Kim, S., Delgado, O., Souza, R., Spechler, S., Wright, W. and Shay, J.: Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro.
, Gastroenterol 138,
1012, 2010 | |
| Cells: | Colonic epithelial |
| Enzyme(s) | Collagenase: 150 u/ml Neutral Protease: 0.04 mg/ml |
| Medium | Basal X media |
Colon: Human | |
| |
| Zhou, J., Belov, L., Huang, P., Shin, J., Solomon, M., Chapuis, P., Bokey, L., Chan, C., Clarke, C., Clarke, S. and Christopherson, R.: Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing.
, J Immunol Methods 355,
40, 2010 | |
| Cells: | Colorectal cancer |
| Enzyme(s) | Collagenase Type 4: 1% Deoxyribonuclease I: 0.2% |
| Medium | HBSS |
Colon: Human | |
| |
| Huang, E., Hynes, M., Zhang, T., Ginestier, C., Dontu, G., Appelman, H., Fields, J., Wicha, M. and Boman, B.: Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis.
, Cancer Res 69,
3382-9, 2009 | |
| Cells: | Colonic epithelial |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | not listed |
Colon: Human | |
| |
| Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion.
, EMBO Mol Med
1,
338-51, 2009 | |
| Cells: | Colon cancer |
| Enzyme(s) | Collagenase Type 1: 300 u/ml Hyaluronidase: 100 u/ml |
| Medium | DMEM/F12 |
Colon: Human | |
| |
| Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells
, Proc Natl Acad Sci U S A 104,
10158-63, 2007 | |
| Cells: | Cancer stem cell |
| Enzyme(s) | Collagenase Type 3: 200 u/ml Deoxyribonuclease I: 100 u/ml |
| Medium | RPMI-1640 |
Colon: Human | |
| |
| Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients.
, Am J Physiol Gastrointest Liver Physiol
286,
G804-13, 2004 | |
| Cells: | Colonic epithelial |
| Enzyme(s) | Collagenase: Neutral Protease: 0.3% Deoxyribonuclease I: 0.05% |
| Medium | RPMI 1640 |
Colon: Human | |
| |
| Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells
, Am J Physiol/Gastro 282,
G1088, 2002 | |
| Cells: | Colonic endothelial cells |
| Enzyme(s) | Collagenase Type 2: 0.25% |
| Medium | HBSS/5%FBS |
Colon: Human | |
| |
| Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes
, J Nutr 46,
96-105, 2001 | |
| Cells: | Colonocytes |
| Enzyme(s) | Collagenase: 0.15% |
| Medium | DMEM/F12 |
Colon: Human | |
| |
| Hisamatsu, T., Watanabe, M., Ogata, H., Ezaki, T., Hozawa, S., Ishii, H., Kanai, T. and Hibi, T.: Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis.
, Cancer Res 59,
5927-31, 1999 | |
| Cells: | Epithelial and mucosal lymphocytes |
| Enzyme(s) | Neutral Protease: 0.1% CLSPA: 0.02% Deoxyribonuclease I: 0.01% |
| Medium | RPMI 1640 |
Colon: Human | |
| |
| Gibson, P., Rosella, O., Wilson, A., Mariadason, J., Rickard, K., Byron, K. and Barkla, D.: Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate.
, Carcinogenesis
20,
539, 1999 | |
| Cells: | Colonic epithelial |
| Enzyme(s) | Neutral Protease: 1.2 u/ml Collagenase Type 4: 50 u/ml |
| Medium | HBSS |
Colon: Human | |
| |
| Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y: High Fas ligand expression on lymphocytes in lesions of ulcerative colitis
, Gut 43,
48-55, 1998 | |
| Cells: | Lamina propria lymphocytes |
| Enzyme(s) | Collagenase: 25 u/ml |
| Medium | HBSS |
Colon: Mouse | |
| |
| Larmonier, C., Shehab, K., Laubitz, D., Jamwal, D., Ghishan, F. and Kiela, P.: Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice.
, J Biol Chem 291,
8918-30, 2016 | |
| Cells: | Lamina propria |
| Enzyme(s) | Collagenase: 100 u/ml Deoxyribonuclease I: 0.004% |
| Medium | RPMI 1640 |
Colon: Mouse | |
| |
| Fattahi, F., Steinbeck, J., Kriks, S., Tchieu, J., Zimmer, B., Kishinevsky, S., Zeltner, N., Mica, Y., El-Nachef, W., Zhao, H., de Stanchina, E., Gershon, M., Grikscheit, T., Chen, S. and Studer, L.: Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease.
, Nature
531,
105-9, 2016 | |
| Cells: | Colon organoid |
| Enzyme(s) | Collagenase Type 1: 800 u/ml Neutral Protease: 0.013% |
| Medium | DMEM |
Colon: Mouse | |
| |
| Round, J. and Mazmanian, S.: Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota.
, Proc Natl Acad Sci U S A 107,
12204-9, 2010 | |
| Cells: | Lamina propria lymphocytes |
| Enzyme(s) | Neutral Protease: 3 u/ml Collagenase Type 4: 0.05% |
| Medium | HBSS |
Colon: Mouse | |
| |
| Atarashi, K., Nishimura, J., Shima, T., Umesaki, Y., Yamamoto, M., Onoue, M., Yagita, H., Ishii, N., Evans, R., Honda, K. and Takeda, K.: ATP Drives Lamina Propria T(H)17 Cell Differentiation.
, Nature
455,
808, 2008 | |
| Cells: | Lamina propria |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 0.1% Deoxyribonuclease I: 0.004% |
| Medium | RPMI 1640 |
Colon: Mouse | |
| |
| Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells
, Proc Natl Acad Sci U S A 104,
10158-63, 2007 | |
| Cells: | Cancer stem cell |
| Enzyme(s) | Collagenase Type 3: 200 u/ml Deoxyribonuclease I: 100 u/ml |
| Medium | RPMI-1640 |
Colon: Mouse | |
| |
| Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue
, Nat Protoc
2,
2307-11, 2007 | |
| Cells: | Lamina propria mononuclear cells |
| Enzyme(s) | Collagenase: 0.05% Deoxyribonuclease I: 0.05% Neutral Protease: 0.3% |
| Medium | HBSS |
Colon: Mouse | |
| |
| Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells.
, Proc Natl Acad Sci U S A 104,
17022, 2007 | |
| Cells: | Dentritic |
| Enzyme(s) | Collagenase: 300 u/ml Deoxyribonuclease I: 0.002% |
| Medium | RPMI 1640 |
Colon: Mouse | |
| |
| Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.: Essential role for CD103 in the T cell-mediated regulation of experimental colitis
, J Exp Med 202,
1051, 2005 | |
| Cells: | Lympocytes |
| Enzyme(s) | Collagenase/Dispase: 100 u/ml |
| Medium | RPMI 1640 |
Colon: Mouse | |
| |
| Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M: Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis
, Am J Physiol/Gastro 284,
G595-603, 2003 | |
| Cells: | Lamina propria lymphocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% Deoxyribonuclease I: 0.01% |
| Medium | HBSS |
Colon: Mouse | |
| |
| Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA
, J Immunol 168(1),
411, 2002 | |
| Cells: | Lamina propria mononuclear cells |
| Enzyme(s) | Collagenase Type 2: 0.015% Deoxyribonuclease I: 0.01% |
| Medium | RPMI |
Colon: Rat | |
| |
| Somara, S., Bashllari, D., Gilmont, R. and Bitar, K.: Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA.
, Am J Physiol Gastrointest Liver Physiol
300,
G1022, 2011 | |
| Cells: | Colon smooth muscle |
| Enzyme(s) | Soybean Trypsin Inhibitor: 0.01% Collagenase Type 2: 0.1% |
| Medium | DMEM |
Endothelial: Bovine | |
| |
| Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina: The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin
, J Vasc Res 44,
19-30, 2007 | |
| Cells: | Bovine umbilical cord (BUVEC) |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Dulbecco's/Ham F-12 |
Endothelial: Bovine | |
| |
| Yu, M., McAndrew, R., Al-Saghir, R., Maier, K., Medhora, M., Roman, R. and Jacobs, E.: Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries.
, J Appl Physiol 93,
1391, 2002 | |
| Cells: | Pulmonary artery endothelial and smooth muscle cells |
| Enzyme(s) | Collagenase: 0.04-0.05% Soybean Trypsin Inhibitor: 0.04% |
| Medium | RPMI-1640 |
Endothelial: Bovine | |
| |
| Del Vecchio, P., Siflinger-Birnboim, A., Belloni, P., Holleran, L., Lum, H., and Malik, A.: Culture and Characterization of Pulmonary Microvascular Endothelial Cells
, In Vitro Cell Dev Biol 28A,
711, 1992 | |
| Cells: | Endothelial Pulmonary |
| Enzyme(s) | Collagenase: 1000 u/ml Collagenase Type 2: 1000 u/ml |
| Medium | PBS, CMF |
Endothelial: Bovine | |
| |
| Vender, R.: Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension
, In Vitro Cell Dev Biol 28A,
403, 1992 | |
| Cells: | Endothelial |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HEPES |
Endothelial: Bovine | |
| |
| Machi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries
, In Vitro Cell Dev Biol 26,
291, 1990 | |
| Cells: | Cerebral artery Endothelial |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | HBSS |
Endothelial: Bovine | |
| |
| DeNucci, G., Gryglewski, R.J., Warner, T.D., and Vane, J.R.: Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled
, Proc Natl Acad Sci U S A 85,
2334, 1988 | |
| Cells: | Endothelial Aortic |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Krebs Ringer solution |
Endothelial: Bovine | |
| |
| Martin, T.: Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells
, Biochim Biophys Acta 962,
282, 1988 | |
| Cells: | Endothelial, pulmonary artery |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | CMF-Dulbecco's PBS |
Endothelial: Bovine | |
| |
| Carson, M.P. and Haudenschild, C.C.: Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures
, In Vitro Cell Dev Biol 22,
344, 1986 | |
| Cells: | Endothelial Aorta |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | PBS |
Endothelial: Bovine | |
| |
| Kinsella, Michael G. and Wight, Thomas N.: Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration
, J Cell Biol
102,
679, 1986 | |
| Cells: | Endothelial Aortic |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Endothelial: Bovine | |
| |
| Gospodarowicz, D., Massoglia, S., Cheng, J., and Fujii, D.: Effect of Fibroblast Growth Factor and Lipoproteins on the Proliferation of Endothelial Cells Derived From Bovine Adrenal Cortex, Brain Cortex, and Corpus Luteum Capillaries
, J Cell Physiol 127,
121, 1986 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | DMEM/Ham's F-12 |
Endothelial: Bovine | |
| |
| Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain
, In Vitro Cell Dev Biol 21,
172, 1985 | |
| Cells: | Endothelial Brain arteries |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | Dulbecco's PBS |
Endothelial: Bovine | |
| |
| Voyta, J., Via, D., Butterfield, C., and Zetter, B.: Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein
, J Cell Biol
99,
2034, 1984 | |
| Cells: | Endothelial Smooth muscle |
| Enzyme(s) | Collagenase: 0.75% |
| Medium | DMEM |
Endothelial: Bovine | |
| |
| Scott, D., Murray, J., and Barnes, M.: Investigation of the Attachment of Bovine Corneal Endothelial Cells
, Exp Cell Res
144,
472, 1983 | |
| Cells: | Endothelial Corneal |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | PBS: DMEM |
Endothelial: Bovine | |
| |
| Lee, S., Douglas, W., Deneke, S., and Fanburg, B.: Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro
, In Vitro 19,
714, 1983 | |
| Cells: | Endothelial Pulmonary artery |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | RPMI 1640 w/1% Fetal Bovine Serum |
Endothelial: Bovine | |
| |
| Robinson, J. and Gospodarowicz, D.: Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells
, J Cell Physiol 117,
368, 1983 | |
| Cells: | Endothelial, Corneal |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | 0.01M Phosphate buffer with 0.02% EDTA 0.9% NaCl (See Reference) |
Endothelial: Bovine | |
| |
| Olander, J., Marasa, J., Kimes, R., Johnston, G., and Feder, J.: An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells
, In Vitro 18,
99, 1982 | |
| Cells: | Endothelial Subclavian vein |
| Enzyme(s) | Collagenase Type 1: 0.10% |
| Medium | PBS |
Endothelial: Bovine | |
| |
| Makarski, J. S.: Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells
, In Vitro 17,
450, 1981 | |
| Cells: | Aortic Pulmonary artery |
| Enzyme(s) | Collagenase Type 2: 0.10% |
| Medium | PBS |
Endothelial: Bovine | |
| |
| Rosen, E., Mueller, S., Noveral, J., and Levine, E.: Proliferative Characteristics of Clonal Endothelial Cell Strains
, J Cell Physiol 107,
123, 1981 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 1: 0.25% |
| Medium | PBS |
Endothelial: Bovine | |
| |
| Cotta-Pereira, G., Sage, H., Bornstein, P., Ross, R., and Schwartz, S.: Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis
, J Cell Physiol 102,
183, 1980 | |
| Cells: | Aorta |
| Enzyme(s) | Collagenase Type 1: 125 u/ml |
| Medium | Dulbecco's PBS with calcium and magnesium |
Endothelial: Bovine | |
| |
| Ryan, U., Mortara, M., and Whitaker, C.: Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes
, Tissue Cell
12,
619, 1980 | |
| Cells: | Pulmonary artery |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Medium 199 |
Endothelial: Bovine | |
| |
| Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells
, Proc Natl Acad Sci U S A 76,
5217, 1979 | |
| Cells: | Foreskin |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Dulbecco's MEM w/10% calf serum |
Endothelial: Bovine | |
| |
| Eskin, S., Sybers, H., Trevino, L., Lie, J., and Chimoskey, J.: Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein
, In Vitro 14,
903, 1978 | |
| Cells: | Saphenous Vein Aorta |
| Enzyme(s) | Collagenase: 0.01% |
| Medium | PBS |
Endothelial: Bovine | |
| |
| Schwartz, S.M.: Selection and Characterization of Bovine Aortic Endothelial Cells
, In Vitro 14,
966, 1978 | |
| Cells: | Endothelial Thoracic aorta Saphenous veins |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | PBS |
Endothelial: Bovine | |
| |
| Ryan, U.S., Clements, E., Habliston, D., and Ryan, J.W.: Isolation And Culture of Pulmonary Artery Endothelial Cells
, Tissue Cell
10,
535, 1978 | |
| Cells: | Pulmonary artery |
| Enzyme(s) | Collagenase Type 2: 0.25% |
| Medium | Puck's solution |
Endothelial: Bovine | |
| |
| Howard, B., Macarak, E., Gunson, D., and Kefalides, N.: Characterization of the Collagen Synthesized by Endothelial Cells in Culture
, Proc Natl Acad Sci U S A 73 (7),
2361, 1976 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Endothelial: Canine | |
| |
| Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels
, Brain Res Bull 21,
785, 1988 | |
| Cells: | Microvessels |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | DMEM |
Endothelial: Canine | |
| |
| Ford, J., Burkel, W., and Kahn, R.: Isolation of Adult Canine Venous Endothelium for Tissue Culture
, In Vitro 17,
44, 1981 | |
| Cells: | Endothelium Jugular vein |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Earle's PBS, CMF |
Endothelial: Guinea-Pig | |
| |
| Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium
, Am J Physiol Heart Circ Physiol
281,
H1657-66, 2001 | |
| Cells: | Coronary endothelial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | see reference |
Endothelial: Human | |
| |
| Ganguly, A., Zhang, H., Sharma, R., Parsons, S. and Patel, K.: Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions.
, J Vis Exp
66,
e4032, 2012 | |
| Cells: | Vascular endothelial |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Endothelial: Human | |
| |
| Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells
, Inv Ophthal Visual Sci 48,
614-20, 2007 | |
| Cells: | Corneal endothelial |
| Enzyme(s) | Collagenase: 0.2% Neutral Protease: 1.0% |
| Medium | DMEM/F12 |
Endothelial: Human | |
| |
| Moss, S., Bates, M., Parrino, P. and Woods, TC.: Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue.
, Ochsner J
7,
133, 2007 | |
| Cells: | Endothelial and vascular smooth muscle |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Endothelial: Human | |
| |
| Davis, J., Crampton, S. and Hughes. C.: Isolation of Human Umbilical Vein Endothelial Cells (HUVEC).
, J Vis Exp
3,
183, 2007 | |
| Cells: | HUVEC |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Endothelial: Human | |
| |
| Silva AP, Kaufmann JE, Vivancos C, Fakan S, Cavadas C, Shaw P, Brunner HR, Vischer U, and Grouzmann E.: Neuropeptide Y expression, localization and cellular transducing effects in HUVEC
, Biol Cell 97(6),
457, 2005 | |
| Cells: | HUVEC |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | RPMI 1640 |
Endothelial: Human | |
| |
| Patel, V., Logan, A., Watkinson, J., Uz-Zaman, S., Sheppard, M., Ramsden, J. and Eggo, M.: Isolation and Characterization of Human Thyroid Endothelial Cells.
, Am J Physiol Endocrinol Metab
284,
E168, 2003 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Endothelial: Human | |
| |
| Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation.
, Am J Physiol Gastrointest Liver Physiol
285,
G1277, 2003 | |
| Cells: | Esophageal microvascular endothelial |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | MCDB-131 |
Endothelial: Human | |
| |
| Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells
, Am J Physiol/Gastro 282,
G1088, 2002 | |
| Cells: | Colonic endothelial cells |
| Enzyme(s) | Collagenase Type 2: 0.25% |
| Medium | HBSS/5%FBS |
Endothelial: Human | |
| |
| Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM, and Kirkpatrick CJ.: Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro
, Exp Mol Pathol 72,
221, 2002 | |
| Cells: | Pulmonary vascular endothelial cells |
| Enzyme(s) | Neutral Protease: 1.18 u/ml Elastase: 10 u/ml |
| Medium | M199 |
Endothelial: Human | |
| |
| Takano Manabu, Meneshian Avedis, Sheikh Emran, Yamakawa Yasuhiko, Wilkins Kirsten Bass, Hopkins Elise A, Bulkley Gregory B: Rapid upregulation of endothelial P-selectin expression via reactive oxygen species generation
, Am J Physiol Heart Circ Physiol
283,
H2054-61, 2002 | |
| Cells: | HUVEC |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | PBS |
Endothelial: Human | |
| |
| Goolcharran, C., Cleland, J., Keck, R., Jones, A., and Borchardt, R.: Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides
, AAPS PharmSci 2 (1),
5, 2000 | |
| Cells: | Endothelial |
| Enzyme(s) | Trypsin: 2% |
| Medium | PBS |
Endothelial: Human | |
| |
| Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells
, Circulation 101(19),
2317, 2000 | |
| Cells: | HUVEC, porcine pulmonary arterial endothelial cells |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Endothelial: Human | |
| |
| Schonbeck U, Sukhova GK, Graber P, Coulter S, Libby P: Augmented expression of cyclooxygenase-2 in human atherosclerotic lesions
, Am J Pathol 155,
1281-91, 1999 | |
| Cells: | Vascular endothelial cells |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | DMEM |
Endothelial: Human | |
| |
| Sanyal AJ, and Mirshahi F.: A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts
, Lab Invest 78(11),
1469, 1998 | |
| Cells: | Hepatic endothelial cells |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM |
Endothelial: Human | |
| |
| Wojta J, Gallicchio M, Zoellner H, Filonzi E, Hamilton J, McGrath K: Interleukin-4 stimulates expression of urokinase-type-plasminogen activator in cultured human foreskin microvascular endothelial cells
, Blood 81,
3285-92, 1993 | |
| Cells: | Foreskin microvascular endothelial |
| Enzyme(s) | Trypsin: 0.3% |
| Medium | HBSS |
Endothelial: Human | |
| |
| Lee, K, Lawley, T, Xu, Y, and Swerlick, R.: VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells
, J Invest Dermatol 98,
79, 1992 | |
| Cells: | Endothelial |
| Enzyme(s) | Trypsin: 0.3.% |
| Medium | HBSS (see reference) |
Endothelial: Human | |
| |
| Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ: Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis
, Am J Physiol 262,
H1088-95, 1992 | |
| Cells: | Vascular endothelial |
| Enzyme(s) | Neutral Protease: 0.15% Trypsin: 0.25% |
| Medium | M-199 |
Endothelial: Human | |
| |
| Grant, D.S., Lelkes, P.I., Fukuda, K., Kleinman, H.K.: Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro
, In Vitro Cell Dev Biol 27,
327, 1991 | |
| Cells: | Umbilical vein HUVEC |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Cord Buffer (See Reference) |
Endothelial: Human | |
| |
| Whitehead, R., and Eeden, P.: A Method For the Prolonged Culture of Colonic Epithelial Cells, J Tiss Cul Meth 13,
103, 1991 | |
| Cells: | Crypt cells |
| Enzyme(s) | Collagenase: 125 u/ml |
| Medium | RMPI 1640 |
Endothelial: Human | |
| |
| Fischer, E., Stingl, A., and Kirkpatrick, C.: Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids
, J Immunol Methods 128,
235, 1990 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | Medium 199 |
Endothelial: Human | |
| |
| Muller WA, Ratti CM, McDonnell SL, Cohn ZA: A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions
, J Exp Med 170,
399-414, 1989 | |
| Cells: | Human umbilical vein endothelial cells |
| Enzyme(s) | Collagenase Type 2: 75 u/ml |
| Medium | M199 |
Endothelial: Human | |
| |
| Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels
, Brain Res Bull 21,
785, 1988 | |
| Cells: | Microvessels |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | DMEM |
Endothelial: Human | |
| |
| Kubota, Y., Kleinman, H., Martin, G., and Lawley, T.: Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures
, J Cell Biol
107,
1589, 1988 | |
| Cells: | Endothelial/HUVEC Foreskin & umbilical cord |
| Enzyme(s) | Trypsin: 0.3% |
| Medium | HBSS/PBS, Medium 199 (see reference) |
Endothelial: Human | |
| |
| Hoshi, H., Kan, M., Chen, J., and McKeehan, W.: Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells
, In Vitro Cell Dev Biol 24 (4),
309, 1988 | |
| Cells: | Umbilical cord Smooth muscle |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HEPES |
Endothelial: Human | |
| |
| Sharefkin, J.B., Fairchild, K.D., Albus, R.A., Cruess, D.F., and Rich, N.M.: The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques
, J Surg Res 41,
463, 1986 | |
| Cells: | Endothelial Saphenous vein |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | PBS, CMF |
Endothelial: Human | |
| |
| Hoshi, H., and McKeehan, W.: Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium
, In Vitro Cell Dev Biol 22 (1),
51, 1986 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HEPES |
Endothelial: Human | |
| |
| Marks, R.M., Czerniecki, M., and Penny, R.: Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture
, In Vitro Cell Dev Biol 21,
627, 1985 | |
| Cells: | Endothelial Dermal |
| Enzyme(s) | Trypsin: 0.3% |
| Medium | PBS |
Endothelial: Human | |
| |
| Gordon, P., Sussman, I., and Hatcher, V.: Long-Term Culture of Human Endothelial Cells
, In Vitro 19,
661, 1983 | |
| Cells: | Fibroblasts Foreskin |
| Enzyme(s) | Hyaluronidase: 0.10% |
| Medium | DMEM |
Endothelial: Human | |
| |
| Glassberg, M., Bern, M., Coughlin, S., Haudenschild, C., Hoyer, L., and Antoniades, H.: Cultured Endothelial Cells Derived from the Human Iliac Arteries
, In Vitro 18,
859, 1982 | |
| Cells: | Iliac arteries |
| Enzyme(s) | Collagenase: 0.25% |
| Medium | PBS w/Ca++, Mg++, & BSA (see reference) |
Endothelial: Human | |
| |
| Ashida, E., Johnson, A., and Lipsky, P.: Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro
, J Clin Invest 67,
1490, 1981 | |
| Cells: | Peripheral blood mononuclear Monocytes T cells Endothelial |
| Enzyme(s) | Collagenase: 0.25% |
| Medium | RPMI 1640 |
Endothelial: Human | |
| |
| Sherer, G., Fitzharris, T., Faulk, W., and LeRoy, E.: Cultivation of Microvascular Endothelial Cells from Human Preputial Skin
, In Vitro 16,
675, 1980 | |
| Cells: | Microvascular endothelial Neonatal foreskins |
| Enzyme(s) | Neutral Protease: at 1000 u/ml |
| Medium | Konigsberg's modification of HBSS (See Reference) |
Endothelial: Human | |
| |
| Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells
, Proc Natl Acad Sci U S A 76,
5217, 1979 | |
| Cells: | Foreskin |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Dulbecco's MEM w/10% calf serum |
Endothelial: Human | |
| |
| Jaffe, E.A., Minick, C.R., Adelman, B., Becker, C.G., and Nachman, R.: Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells
, J Exp Med 144,
209, 1976 | |
| Cells: | Umbilical vein |
| Enzyme(s) | Trypsin: 100 µg/ml |
| Medium | Tris-HCl,0.2 M |
Endothelial: Human | |
| |
| Gimbrone Jr., M.A.: Culture of Vascular Endothelium
, Prog Hemost Thromb 3,
1, 1976 | |
| Cells: | Umbilical vein |
| Enzyme(s) | Collagenase Type 1: 125 u/ml |
| Medium | Dulbecco's PBS |
Endothelial: Human | |
| |
| Gimbrone, M.A., Cotran, R.S., and Folkman, J.: Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis
, J Cell Biol
60,
673, 1974 | |
| Cells: | Umbilical cord |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Dulbecco's PBS |
Endothelial: Human | |
| |
| Jaffe, E., Nachman, R., Becker, C., and Minick, C.: Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria
, J Clin Invest 52,
2745, 1973 | |
| Cells: | Umbilical cord |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Cord buffer (See Reference) |
Endothelial: Human | |
| |
| Lewis, L.J., Haok, J.C., Maca, R.D., and Fry, G.L.: Replication of Human Endothelial Cells in Culture
, Science 181,
452, 1973 | |
| Cells: | Umbilical cord |
| Enzyme(s) | Trypsin NF 1:250: 0.125% |
| Medium | Saline, normal |
Endothelial: Human | |
| |
| Fryer, D.G., Birnbaum, G., and Luttrell, C.N.: Human Endothelium in Cell Culture
, J Atheroscler Res 6,
151, 1966 | |
| Cells: | Umbilical cord |
| Enzyme(s) | Trypsin NF 1:250: 0.25% |
| Medium | CMF solution |
Endothelial: Mouse | |
| |
| Sabbagh, M. and Nathans, J.: A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture
, Elife
,
1, 2020 | |
| Cells: | Endothelial, CNS |
| Enzyme(s) | PDS kit: with modifications Stemxyme 1: 0.1% |
| Medium | EC growth medium |
Endothelial: Mouse | |
| |
| Esteban, S., Clemente, C., Koziol, A., Gonzalo, P., Rius, C., Martinez, F., Linares, P., Chaparro, M., Urzainqui, A., Andres, V., Seiki, M., Gisbert, J and Arroyo, A.: Endothelial MT1-MMP Targeting Limits Intussusceptive Angiogenesis and Colitis via TSP1/Nitric Oxide Axis.
, EMBO Mol Med
12,
e10862, 2020 | |
| Cells: | Aortic endothelial |
| Enzyme(s) | Collagenase Type 1: 0.6% Elastase: 0.25% |
| Medium | DMEM |
Endothelial: Mouse | |
| |
| Luo, S., Truong, A. and Makino, A.: Isolation of Mouse Coronary Endothelial Cells.
, J Vis Exp
,
, 2016 | |
| Cells: | Coronary endothelial |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 0.6 u/ml |
| Medium | Kreb's |
Endothelial: Mouse | |
| |
| Wang, J., Sun, C., Gerdes, N., Liu, C., Liao, M., Liu, J., Shi, M., He, A., Zhou, Y., Sukhova, G., Chen, H., Cheng, X., Kuzuya, M., Murohara, T., Zhang, J., Cheng, X., Jiang, M., Shull, G. and Rogers, S.: Interleukin 18 Function in Atherosclerosis is Mediated by the Interleukin 18 Receptor and the Na-Cl Co-Transporter.
, Nat Med 21,
820-6, 2015 | |
| Cells: | Endothelial lung |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM |
Endothelial: Mouse | |
| |
| Xiao, L., McCann, J. and Dudley, A.: Isolation and Culture Expansion of Tumor-specific Endothelial Cells.
, J Vis Exp
,
e53072, 2015 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 0.25 u/ml Deoxyribonuclease I: 0.0075% |
| Medium | DMEM |
Endothelial: Mouse | |
| |
| Kochukov, M., Balasubramanian, A., Abramowitz, J., Birnbaumer, L. and Marrelli, S.: Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery.
, J Am Heart Assoc
3,
, 2014 | |
| Cells: | Cerebral artery endothelial |
| Enzyme(s) | Neutral Protease: 4 u/ml Elastase: 1 u/ml Collagenase Type 1: 120 u/ml |
| Medium | DMEM |
Endothelial: Mouse | |
| |
| Kazerounian, S., Gerald, D., Huang, M., Chin, R, Udayakumar, D, Zheng, N., O'Donnell, R., Perruzzi, C., Mangiante, L., Pourat, J., Phung, T., Bravo-Nuevo, A., Shechter, S., McNamara, S. and Duhadaway, K.: RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis.
, Cancer Res 73,
50, 2013 | |
| Cells: | Tumor endothelial |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Endothelial: Mouse | |
| |
| Imoukhuede, P. and Popel, A.: Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle.
, PLoS ONE
7,
e44791, 2012 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | HBSS |
Endothelial: Mouse | |
| |
| Daneman, R., Zhou, L., Agalliu, D., Cahoy, J., Kaushal, A. and Barres, B.: The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells.
, PLoS ONE
5,
e13741, 2010 | |
| Cells: | Endothelial |
| Enzyme(s) | Papain: 40 u/ml Deoxyribonuclease I: 125 u/ml |
| Medium | DPBS |
Endothelial: Mouse | |
| |
| Follenzi, A., Benten, D., Novikoff, P., Faulkner, L., Raut, S. and Gupta, S.: Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice.
, J Clin Invest 118,
935, 2008 | |
| Cells: | Liver endothelial |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Endothelial: Mouse | |
| |
| Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis.
, Am J Physiol Cell Physiol
292,
C2070, 2007 | |
| Cells: | Endothelial kidney |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Endothelial: Mouse | |
| |
| Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong: Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation
, J Cell Biol
172,
151-62, 2006 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 3: 200 u/ml Deoxyribonuclease I: 0.001% |
| Medium | PBS |
Endothelial: Mouse | |
| |
| Cha, S., Talavera, D., Demir, E., Nath, A. and Sierra-Honigmann, M.: A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin.
, Microvasc Res 70,
198, 2005 | |
| Cells: | Microvascular endothelial |
| Enzyme(s) | Neutral Protease: 0.005% Collagenase Type 1: 4% |
| Medium | DMEM |
Endothelial: Mouse | |
| |
| Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells
, Mol Vis
9,
171, 2003 | |
| Cells: | Retinal endothelial cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Endothelial: Mouse | |
| |
| Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K, and Miyasaka M: Expression profile of active genes in mouse lymph node high endothelial cells
, Int Immunol 11(12),
1989, 1999 | |
| Cells: | Endothelial cells from lymph node |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Endothelial: Mouse | |
| |
| Sapatino, B., Welsh, C., Smith, C., Bebo, B., and Linticum, D.: Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme
, In Vitro Cell Dev Biol 29A,
923, 1993 | |
| Cells: | Cerebrovascular Endothelial |
| Enzyme(s) | Collagenase/Dispase: 0.1% |
| Medium | PBS |
Endothelial: Porcine | |
| |
| Balaoing, L., Post, A., Lin, A., Tseng, H., Moake, J. and Grande-Allen, K.: Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation.
, PLoS ONE
10,
e0130749, 2015 | |
| Cells: | Valvular endothelial |
| Enzyme(s) | Neutral Protease: 2 u/ml Collagenase Type 2: 60 u/ml |
| Medium | PBS |
Endothelial: Porcine | |
| |
| Gould, R. and Butcher, J.: Isolation of Valvular Endothelial Cells.
, J Vis Exp
46,
e2158, 2010 | |
| Cells: | Valvular endothelial |
| Enzyme(s) | Collagenase Type 2: 300-600 u/ml |
| Medium | DMEM |
Endothelial: Porcine | |
| |
| Nugent, H., and Edelman, E.: Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury
, J Surg Res 99,
228, 2001 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 1: |
| Medium | DMEM w/ 10% calf serum |
Endothelial: Porcine | |
| |
| Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells
, Circulation 101(19),
2317, 2000 | |
| Cells: | HUVEC, porcine pulmonary arterial endothelial cells |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Endothelial: Porcine | |
| |
| Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM, and Tisher CC.: Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV
, J Am Soc Nephrol
10(3),
481, 1999 | |
| Cells: | Porcine pulmonary endothelial |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | RPMI 1640 |
Endothelial: Porcine | |
| |
| Shasby, S.: Endothelial Cells Grown On Permeable Membrane Supports, J Tiss Cul Meth 14,
247, 1992 | |
| Cells: | Endothelial |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Medium 199 |
Endothelial: Porcine | |
| |
| Vischer, P., and Buddecke, E.: Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells
, Exp Cell Res
158,
15, 1985 | |
| Cells: | Endothelial |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Endothelial: Porcine | |
| |
| Dickinson, E. and Slakey, L.: Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta
, In Vitro 18,
63, 1982 | |
| Cells: | Endothelial Aortic |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | Dulbecco-Vogt MEM w/o serum |
Endothelial: Porcine | |
| |
| Merrilees, M.J., and Scott, L.: Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture
, Atherosclerosis 39,
147, 1981 | |
| Cells: | Endothelial Thoracic aorta |
| Enzyme(s) | Collagenase Type 4: 0.025% Trypsin: 0.05% |
| Medium | Medium 199 and 0.01M EDTA |
Endothelial: Porcine | |
| |
| Slater, D.N., and Sloan, J.M.: The Porcine Endothelial Cell in Tissue Culture
, Atherosclerosis 21,
259, 1975 | |
| Cells: | Endothelial Aortas Veins |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Medium 199 w/BSS and HEPES or NaHCO3 |
Endothelial: Porcine | |
| |
| Coulson, W.F.: The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin
, Biochim Biophys Acta 237,
378, 1971 | |
| Cells: | Aorta |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Phosphate buffer (see reference) |
Endothelial: Rabbit | |
| |
| Choi, J., Ko, M., and Kay, E.: Subcellular Localization of the Expressed 18 kDa FGF-2 Isoform in Corneal Endothelial Cells
, Mol Vis
6,
222, 2000 | |
| Cells: | Corneal endothelial cells (CEC) |
| Enzyme(s) | Hyaluronidase: 0.05% |
| Medium | DMEM |
Endothelial: Rabbit | |
| |
| Haley, N., Shio, H., Fowler, S.: Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation
, Lab Invest 37,
287, 1977 | |
| Cells: | Endothelial, aortic |
| Enzyme(s) | Elastase: 0.2% |
| Medium | Hanks solution |
Endothelial: Rat | |
| |
| Schwertschlag, U.S., and Whorton, A.R.: Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells
, J Biol Chem 263,
13791, 1988 | |
| Cells: | Smooth muscle, aorta |
| Enzyme(s) | Soybean Trypsin Inhibitor: 0.25% |
| Medium | HBSS with 0.2 mM Ca++ |
Endothelial: Rat | |
| |
| Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan
, Anal Biochem
161,
207, 1987 | |
| Cells: | Lipocytes Kupffer Sinusoidal endothelial |
| Enzyme(s) | Collagenase: 0.015% |
| Medium | DMEM/Ham's F-12 |
Endothelial: Rat | |
| |
| Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells
, J Biol Chem 258,
12221, 1983 | |
| Cells: | Endothelial Kupffer Parenchymal |
| Enzyme(s) | Collagenase: 0.05% Pronase: 0.25% |
| Medium | HBSS |
Endothelial: Rat | |
| |
| Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.: Primary Culture of Rat Cerebral Microvascular Endothelial Cells
, Lab Invest 46,
554, 1982 | |
| Cells: | Endothelial Cerebral |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | HBSS |
Endothelial: Rat | |
| |
| Merrilees, M.J., and Scott, L.: Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture
, Atherosclerosis 39,
147, 1981 | |
| Cells: | Endothelial Thoracic aorta |
| Enzyme(s) | Collagenase Type 4: 0.025% Trypsin: 0.05% |
| Medium | Medium 199 and 0.01M EDTA |
Endothelial: Rat | |
| |
| Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells
, Proc Natl Acad Sci U S A 76,
5217, 1979 | |
| Cells: | Foreskin |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Dulbecco's MEM w/10% calf serum |
Endothelial: Rat | |
| |
| Phillips, P., Kumar, P., Kumar, S., and Waghe, M.: Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter
, J Anat 129,
261, 1979 | |
| Cells: | Endothelial |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | BSS |
Epithelial: Bovine | |
| |
| Schumann, B.L., Cody, T.E., Miller, M.L., Leikauf, G.D.: Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells
, In Vitro Cell Dev Biol 24,
211, 1988 | |
| Cells: | Epithelial Tracheal |
| Enzyme(s) | Neutral Protease: 2% |
| Medium | Dissociation medium, CMF |
Epithelial: Canine | |
| |
| Virmani, A., Naziruddin, B., Desai, V., Lowry, J., Graves, D., and Sachdev, G.: Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion
, In Vitro Cell Dev Biol 28A,
120, 1992 | |
| Cells: | Tracheal |
| Enzyme(s) | Pronase: 0.1% |
| Medium | DMEM |
Epithelial: Chicken | |
| |
| Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12A:3.1, 1995 | |
| Cells: | Intestinal mesenchymal and epithelial |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Epithelial: Fish | |
| |
| Karnaky, Jr., K.J., Valentich, J.D., Currie, M.G., Oehlenschlager, W.F., and Kennedy, M.P.: Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells
, Am J Physiol 260,
1125, 1991 | |
| Cells: | Rectal gland |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Ringer's solution |
Epithelial: Fish | |
| |
| Valentich, J: Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport, J Tiss Cul Meth 13,
149, 1991 | |
| Cells: | Rectal gland |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Ringer's solution |
Epithelial: Fish | |
| |
| Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport
, Am J Physiol 251,
424, 1986 | |
| Cells: | Renal tubule |
| Enzyme(s) | Trypsin: 0.2% Trypsin: 0.2% |
| Medium | CMF solution |
Epithelial: Frog | |
| |
| Heinke, B, and Clauss, W.: Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium
, J Comp Physiol [B] 169,
148, 1999 | |
| Cells: | Colonic epithelial |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | Kreb's |
Epithelial: Guinea-Pig | |
| |
| Rutten, M.: Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells, J Tiss Cul Meth 14,
235, 1992 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Epithelial: Guinea-Pig | |
| |
| Chaminadas, G., Alkhalaf, M., Remy-Martin, J.P., Propper, A.Y., and Adessi, G.L.: Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium
, J Endocrinol 123,
233, 1989 | |
| Cells: | Endometrial |
| Enzyme(s) | Collagenase: 0.25% |
| Medium | HBSS |
Epithelial: Hamster | |
| |
| Niles,R., Kim, K.C., Hyman, B., Christensen, T., Wasano, K., Brody, J.: Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells
, In Vitro Cell Dev Biol 24,
457, 1988 | |
| Cells: | Tracheal |
| Enzyme(s) | Pronase: 0.1% |
| Medium | MEM with Hepes, CMF |
Epithelial: Hamster | |
| |
| McDowell, E., et al.: Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters
, Am J Pathol 129,
511, 1987 | |
| Cells: | Tracheal |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Ham's F-12 |
Epithelial: Hamster | |
| |
| Lee, T., Wu, R., Brody, A., Barrett, J., and Nettesheim, P.: Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture
, Exp Lung Res 6,
27, 1984 | |
| Cells: | Tracheal |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Medium 199 |
Epithelial: Hamster | |
| |
| Goldman, W.E., Baseman, J.B.: Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea
, In Vitro 16,
313, 1980 | |
| Cells: | Tracheal |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS with EDTA |
Epithelial: Human | |
| |
| Gottipamula, S., Saraswat, S. and Sridhar, K.: Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells.
, Cytotherapy
19,
263-271, 2017 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 4: 0.1% Neutral Protease: 0.1% |
| Medium | PBS |
Epithelial: Human | |
| |
| Hsueh, Y., Huang, S., Lai, J., Ma, S., Chen, H., Wu, S., Wang, T., Sun, C., Ma, K., Chen, J., Lai, C. and Ma, D.: Preservation of Epithelial Progenitor Cells from Collagenase-Digested Oral Mucosa During Ex Vivo Cultivation.
, Sci Rep
6,
36266, 2016 | |
| Cells: | Oral mucosal epithelial |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | DMEM/F12 |
Epithelial: Human | |
| |
| VanDussen, K., Marinshaw, J., Shaikh, N., Miyoshi, H., Moon, C., Tarr, P., Ciorba, M. and Stappenbeck, T.: Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays.
, Gut 64,
911-20, 2015 | |
| Cells: | Gastrointestinal epithelial |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F12 |
Epithelial: Human | |
| |
| Widdicombe JH, Sachs LA, Morrow JL, and Finkbeiner WE.: Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function
, Biotechniques
39(2),
249, 2005 | |
| Cells: | Human tracheal epithelium |
| Enzyme(s) | Protease Type XIV: 0.04% |
| Medium | DMEM/F12 |
Epithelial: Human | |
| |
| Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients.
, Am J Physiol Gastrointest Liver Physiol
286,
G804-13, 2004 | |
| Cells: | Colonic epithelial |
| Enzyme(s) | Collagenase: Neutral Protease: 0.3% Deoxyribonuclease I: 0.05% |
| Medium | RPMI 1640 |
Epithelial: Human | |
| |
| Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet
, Inv Ophthal Visual Sci 44(10),
4275, 2003 | |
| Cells: | Corneal limbal epithelial sheet |
| Enzyme(s) | Neutral Protease: 5% |
| Medium | see reference |
Epithelial: Human | |
| |
| Fahlgren, A., Hammarstrom, S., Danielsson, A. and Hammarstrom, M.: Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis.
, Clin Exp Immunol 131,
90, 2003 | |
| Cells: | Intestinal epithelial |
| Enzyme(s) | Collagenase Type 4: 72.5 u/ml |
| Medium | HBSS |
Epithelial: Human | |
| |
| Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22,
133, 2000 | |
| Cells: | Gastric epithelial cells |
| Enzyme(s) | Collagenase Type 2: 200 u/ml Neutral Protease: 1.2 u/ml Soybean Trypsin Inhibitor: 0.125% |
| Medium | L-15 |
Epithelial: Human | |
| |
| Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R and Little, C: The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12B:10.1, 1995 | |
| Cells: | Gastric |
| Enzyme(s) | Collagenase Type 4: 0.01% |
| Medium | F-12 medium |
Epithelial: Human | |
| |
| Halbert CL, Alexander IE, Wolgamot GM, Miller AD: Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells
, J Virol 69,
1473-9, 1995 | |
| Cells: | Nasal polyp epithelial |
| Enzyme(s) | Neutral Protease: .004% Trypsin: 0.1% |
| Medium | see reference |
Epithelial: Human | |
| |
| Sabatini, L., Allen-Hoffmann, B, Warner, T., and Azen, E.: Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands
, In Vitro Cell Dev Biol 27A,
939, 1991 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Epithelial: Human | |
| |
| Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13,
95, 1991 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | MEM, PBS |
Epithelial: Human | |
| |
| Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples
, In Vitro Cell Dev Biol 26,
1186, 1990 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 2.0% |
| Medium | DMEM/Ham's F-12 |
Epithelial: Human | |
| |
| Gruenert, D.C., Basbaum, C.B., and Widdicombe, J.H.: Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions
, In Vitro Cell Dev Biol 26,
411, 1990 | |
| Cells: | Epithelial |
| Enzyme(s) | Pronase: 0.1% |
| Medium | PBS |
Epithelial: Human | |
| |
| Wood, L. and Neufeld, E.: A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux
, J Biol Chem 265,
12796, 1990 | |
| Cells: | Epithelial Sweat gland |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | (see reference) |
Epithelial: Human | |
| |
| Munson, L., Chandler, S., and Schlafer, D.: Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells, J Tiss Cul Meth 11 (3),
129, 1988 | |
| Cells: | Endometrial epithelial |
| Enzyme(s) | Trypsin: |
| Medium | DMEM/Ham's F-12 |
Epithelial: Human | |
| |
| Widdicombe, J.H., Coleman, D.L., Finkbeiner, W.E., and Tuet, I.K.: Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa
, J Appl Physiol 58,
1729, 1985 | |
| Cells: | Epithelial |
| Enzyme(s) | Deoxyribonuclease I: 0.01% |
| Medium | HEPES with 5.9mM Glucose,5mM DTT |
Epithelial: Human | |
| |
| Yankaskas, J., Cotton, C., Knowles, M., Gatzy, J., and Boucher, R.: Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports
, Am Rev Respir Dis 132,
1281, 1985 | |
| Cells: | Epithelial |
| Enzyme(s) | Protease Type XIV: 0.1% |
| Medium | Eagle's MEM |
Epithelial: Human | |
| |
| Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture
, In Vitro 20,
743, 1984 | |
| Cells: | Epithelial Ovary |
| Enzyme(s) | Trypsin: 0.125% |
| Medium | HBSS, CMF |
Epithelial: Human | |
| |
| Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures
, Methods Cell Biol 21,
195, 1980 | |
| Cells: | Epithelial Prostate |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS |
Epithelial: Mouse | |
| |
| Brann, D., Tsukahara, T., Weinreb, C., Lipovsek, M., Van den Berge, K., Gong, B., Chance, R., Macaulay, I., Chou, H., Fletcher, R., Das, D., Street, K., de Bezieux, H., Choi, Y., Risso, D. and Dudoit, S.: Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia.
, Sci. Adv.
6,
, 2020 | |
| Cells: | Nasal epithelium |
| Enzyme(s) | Papain: 20 u/ml Deoxyribonuclease I: 0.005% |
| Medium | EBSS |
Epithelial: Mouse | |
| |
| Sun, F., Xiao, G. and Qu, Z.: Isolation of Murine Alveolar Type II Epithelial Cells.
, Bio-protocol
7,
, 2017 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Neutral Protease: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Epithelial: Mouse | |
| |
| Pardo-Saganta, A., Tata, P., Law, B., Saez, B., Chow, R., Prabhu, M., Gridley, T. and Rajagopal, J.: Parent Stem Cells Can Serve as Niches for Their Daughter Cells.
, Nature
523,
597-601, 2015 | |
| Cells: | Tracheal epithelial |
| Enzyme(s) | Papain: 50 u/ml |
| Medium | See reference |
Epithelial: Mouse | |
| |
| Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R., Ali, R. and Sowden, J.: Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells.
, Curr Protoc Stem Cell Biol
Chapter 1,
Unit 1H.4, 2011 | |
| Cells: | Cilary epithelial |
| Enzyme(s) | PDS kit: per instructions |
| Medium | EBSS |
Epithelial: Mouse | |
| |
| Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress.
, Oxid Med Cell Longev
1,
33, 2008 | |
| Cells: | Renal tubular epithelial |
| Enzyme(s) | Collagenase: 200 u/ml Soybean Trypsin Inhibitor: see reference |
| Medium | HBSS |
Epithelial: Mouse | |
| |
| Mathew, R., Degenhard,t K., Haramaty, L., Karp, C. and White, E.: Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer.
, Methods Enzymol
446,
77-106, 2008 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 0.025% Neutral Protease: 0.25% |
| Medium | DMEM |
Epithelial: Mouse | |
| |
| Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA
, J Immunol 168(1),
411, 2002 | |
| Cells: | Lamina propria mononuclear cells |
| Enzyme(s) | Collagenase Type 2: 0.015% Deoxyribonuclease I: 0.01% |
| Medium | RPMI |
Epithelial: Mouse | |
| |
| Mueller, S., Clark, J., Myers, P. and Korach, K.: Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha.
, Endocrinology 143,
2357, 2002 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 3: 25 u/ml Hyaluronidase: 0.1% Protease XIV: 0.05% Deoxyribonuclease I: 0.04% |
| Medium | DMEM/F12 |
Epithelial: Mouse | |
| |
| Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y: Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis
, Am J Pathol 155,
173-81, 1999 | |
| Cells: | Salivary gland epithelial |
| Enzyme(s) | Collagenase Type 1: 750 u/ml Hyaluronidase: 500 u/ml |
| Medium | DMEM/F12 |
Epithelial: Mouse | |
| |
| Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12A:3.1, 1995 | |
| Cells: | Intestinal mesenchymal and epithelial |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Epithelial: Mouse | |
| |
| Durban, E: Submandibular Salivary Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12B:2.1, 1995 | |
| Cells: | Submandibular salivary |
| Enzyme(s) | Collagenase Type 2 or 3: 0.16% |
| Medium | DMEM |
Epithelial: Mouse | |
| |
| Katayama, M., Kan, M.: Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation
, In Vitro Cell Dev Biol 27,
533, 1991 | |
| Cells: | Esophageal |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS, CMF |
Epithelial: Mouse | |
| |
| Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes
, In Vitro Cell Dev Biol 27A,
713, 1991 | |
| Cells: | Uterine |
| Enzyme(s) | Trypsin: 0.2% Trypsin: 0.2% |
| Medium | HBSS |
Epithelial: Mouse | |
| |
| Fukamachi, H., and McLachlan, J.: Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum
, In Vitro Cell Dev Biol 27A,
907, 1991 | |
| Cells: | Uterine |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS |
Epithelial: Mouse | |
| |
| Durban, E.M.: Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix
, In Vitro Cell Dev Biol 26,
33, 1990 | |
| Cells: | Epithelial Submandibular salivary gland |
| Enzyme(s) | Collagenase Type 3: 0.16% , 1:1 v/v |
| Medium | DMEM with 15 mM HEPES |
Epithelial: Mouse | |
| |
| Riser, M., Huff, B., and Medina, D.: Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells
, In Vitro 19,
730, 1983 | |
| Cells: | Epithelial |
| Enzyme(s) | Pepsin: 0.1% and 0.05% Pepsin: 0.1% |
| Medium | HBSS with 0.2% EDTA, CMF |
Epithelial: Mouse | |
| |
| Yang, J., Flynn, D., Larson, L., and Hamamoto, S.: Growth in Primary Culture of Mouse Submandibular Epithelial Cells
, In Vitro 18,
435, 1982 | |
| Cells: | Epithelial Submandibular gland |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | HBSS |
Epithelial: Mouse | |
| |
| Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels
, In Vitro 16,
502, 1980 | |
| Cells: | Mammary tumors Epithelial |
| Enzyme(s) | Collagenase: 1.0% |
| Medium | HBSS |
Epithelial: Mouse | |
| |
| Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver
, In Vitro 15,
910, 1979 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 0.10% |
| Medium | DMEM |
Epithelial: Porcine | |
| |
| Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells
, Acta Opthalmol Scand 81(2),
170, 2003 | |
| Cells: | Retinal pigment epithelial cells |
| Enzyme(s) | Collagenase: 2% |
| Medium | DMEM |
Epithelial: Porcine | |
| |
| De Buysscher, E., Kennedy, J., and Mendicino, J.: Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis
, In Vitro 20,
433, 1984 | |
| Cells: | Trachea |
| Enzyme(s) | Neutral Protease: 0.2% |
| Medium | HBSS |
Epithelial: Rabbit | |
| |
| Vidrich, A., Racindranath, R., Farsi, K., and Targan, S.: A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures
, In Vitro Cell Dev Biol 24 (3),
188, 1988 | |
| Cells: | Colon |
| Enzyme(s) | Neutral Protease: 0.3% |
| Medium | PBS |
Epithelial: Rabbit | |
| |
| Chew, C.S., Brown,M.R.: Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa
, Biochim Biophys Acta 888,
116, 1986 | |
| Cells: | Gastric Parietal and chief |
| Enzyme(s) | Collagenase Type 2: 0.08% |
| Medium | Sodium phosphate buffer |
Epithelial: Rabbit | |
| |
| Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium)
, Int J Gynecol Pathol 3,
348, 1984 | |
| Cells: | Mesothelial and surface epithelial Ovaries |
| Enzyme(s) | Trypsin: 0.125%-0.5% |
| Medium | Medium 199 |
Epithelial: Rabbit | |
| |
| Logsdon, C.D., Bisbee, C.A., Rutten, M.J. and Machen, T.E.: Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel
, In Vitro 18,
233, 1981 | |
| Cells: | Epithelial Gastric |
| Enzyme(s) | Collagenase Type 3: 0.10% |
| Medium | HBSS |
Epithelial: Rat | |
| |
| Mei, N., McDaniel, L., Dobrovolsky, V., Guo, X., Shaddock, J., Mittelstaedt, R., Azuma, M., Shelton, S., McGarrity, L., Doerge, D. and Heflich, R.: The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats.
, Toxicol Sci 115,
412, 2010 | |
| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase Type 3: 0.35% |
| Medium | HBSS |
Epithelial: Rat | |
| |
| Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization
, Biol Proced Online 3 (1),
35, 2001 | |
| Cells: | Seminiferous tubules |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Krebs-Ringer bicarbonate buffer (see reference) |
Epithelial: Rat | |
| |
| Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair
, J Cell Sci
112,
243, 1999 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Elastase: 40 u/ml |
| Medium | DMEM |
Epithelial: Rat | |
| |
| Shannon JM, Gebb SA, and Nielsen LD: Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture
, Development
126,
1675, 1999 | |
| Cells: | Tracheal epithelial |
| Enzyme(s) | Collagenase Type 4: 0.05% Neutral Protease: Deoxyribonuclease I: |
| Medium | DMEM/F12 |
Epithelial: Rat | |
| |
| Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12A:3.1, 1995 | |
| Cells: | Intestinal mesenchymal and epithelial |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Epithelial: Rat | |
| |
| Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats
, Inv Ophthal Visual Sci 34(1),
101, 1993 | |
| Cells: | Retinal pigment epithelial cells |
| Enzyme(s) | Collagenase Type 1: 65 u/ml Hyaluronidase: 220 u/ml |
| Medium | CF Hanks with EDTA |
Epithelial: Rat | |
| |
| Klinefelter, G.: A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats, J Tiss Cul Meth 14,
195, 1992 | |
| Cells: | Epididymal epithelial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | HBSS |
Epithelial: Rat | |
| |
| Evans GS, Flint N, Somers AS, Eyden B, and Potten CS.: The development of a method for the preparation of rat intestinal epithelial cell primary cultures
, J Cell Sci
101,
219, 1992 | |
| Cells: | Rat intestinal epithelial |
| Enzyme(s) | Neutral Protease: 0.01% Collagenase: 300 u/ml |
| Medium | DMEM |
Epithelial: Rat | |
| |
| Dial, E., Kao, Y., and Lichtenberger, L.: Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture
, In Vitro Cell Dev Biol 27,
39, 1991 | |
| Cells: | Epithelial Stomach |
| Enzyme(s) | Pronase: 0.15% |
| Medium | Medium 199 |
Epithelial: Rat | |
| |
| Heimann, T., and Githens, S.: Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix
, Pancreas 6 (5),
514, 1991 | |
| Cells: | Interlobular duct fragments |
| Enzyme(s) | Papain: |
| Medium | DMEM/Ham's F-12 |
Epithelial: Rat | |
| |
| Yassin, R., Clearfield, H., Katz, S., and Murthy, S.: Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro
, Peptides
12,
63, 1991 | |
| Cells: | Colon |
| Enzyme(s) | Deoxyribonuclease I: 10 µg/ml |
| Medium | (see reference) |
Epithelial: Rat | |
| |
| Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium
, In Vitro Cell Dev Biol 27A,
625, 1991 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS |
Epithelial: Rat | |
| |
| Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13,
95, 1991 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | MEM, PBS |
Epithelial: Rat | |
| |
| Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina
, Curr Eye Res 10(11),
1081, 1991 | |
| Cells: | Retinal pigment epithelial |
| Enzyme(s) | Neutral Protease: 2% |
| Medium | DMEM |
Epithelial: Rat | |
| |
| Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus
, In Vitro Cell Dev Biol 26,
713, 1990 | |
| Cells: | Epthelial |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | Eagle's MEM Serum-free |
Epithelial: Rat | |
| |
| Chang, L., Wu, R., and Nettesheim, P.: Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture
, J Cell Sci
74,
283, 1985 | |
| Cells: | Tracheal epithelial |
| Enzyme(s) | Pronase: 0.5% |
| Medium | DMEM |
Epithelial: Rat | |
| |
| Babcock, M., Marino, M., Gunning, W., and Stoner, G.: Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells
, In Vitro 19,
403, 1983 | |
| Cells: | Epithelial Esophagus |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | HEPES BSS |
Epithelial: Rat | |
| |
| Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats
, In Vitro 19,
576, 1983 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HBSS CMF |
Epithelial: Rat | |
| |
| Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma
, In Vitro 18,
565, 1982 | |
| Cells: | Epithelial, cancer and tumor |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Eagles's MEM |
Epithelial: Rat | |
| |
| Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels
, Exp Cell Res
131,
363, 1981 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HBSS, CMF |
Epithelial: Rat | |
| |
| Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells
, Exp Cell Res
89,
139, 1974 | |
| Cells: | Epithelial |
| Enzyme(s) | Hyaluronidase: 0.0075% |
| Medium | KCl-NaCl HEPES Buffer |
Epithelial: Rat | |
| |
| Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver
, Exp Cell Res
69,
106, 1971 | |
| Cells: | Epithelial-like |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS |
Eye: Bovine | |
| |
| Terlizzi, V., Kolibabka, M., Burgess, J., Hammes, H. and Harmsen, M.: The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2.
, Stem Cells 36,
240-251, 2018 | |
| Cells: | Retinal endothelial |
| Enzyme(s) | Collagenase Type 4: 0.05% Deoxyribonuclease I: 0.02% Pronase: 0.02% |
| Medium | DMEM |
Eye: Bovine | |
| |
| Bryan, B. and D'Amore, P.: Pericyte Isolation and USe is Endothelial/Pericyte Coculture Models
, Meth Enzymol 443,
315, 2008 | |
| Cells: | Pericyte |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM |
Eye: Bovine | |
| |
| Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina
, In Vitro 18 (7),
626, 1982 | |
| Cells: | Microvascular endothelial |
| Enzyme(s) | Collagenase/Dispase: 0.1% |
| Medium | MEM |
Eye: Chicken | |
| |
| Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G: Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions
, Cell Death Differ
180,
159-68, 2005 | |
| Cells: | Retinal cells |
| Enzyme(s) | Trypsin: 0.005% Deoxyribonuclease I: 0.005% |
| Medium | DMEM/F12 |
Eye: Chicken | |
| |
| Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H: ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent
, Inv Ophthal Visual Sci 43,
3181-9, 2002 | |
| Cells: | Corneal epithelia |
| Enzyme(s) | Collagenase: 0.08% Trypsin: 0.08% |
| Medium | HBSS |
Eye: Chicken | |
| |
| Seigel, G.: The Golden Age of Retinal Cell Culture
, Mol Vis
5,
4, 1999 | |
| Cells: | Retinal |
| Enzyme(s) | Trypsin: 0.6% |
| Medium | DMEM |
Eye: Chicken | |
| |
| Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina
, In Vitro Cell Dev Biol 26,
1073, 1990 | |
| Cells: | Flat, retina |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Tyrode's solution, CMF |
Eye: Fish | |
| |
| Diekmann, H., Kalbhen, P. and Fischer, D.: Characterization of Optic Nerve Regeneration using Transgenic Zebrafish.
, Front Cell Neurosci
9,
118, 2015 | |
| Cells: | Retinal |
| Enzyme(s) | Papain: 10 u/ml |
| Medium | L-15 |
Eye: Fish | |
| |
| Graffe, M., Zenisek, D. and Taraska, J.: A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals.
, J Gen Physiol 146,
109-17, 2015 | |
| Cells: | Retinal Bipolar |
| Enzyme(s) | Papain: 30 u/ml |
| Medium | HEPES |
Eye: Human | |
| |
| Yusoff,B, Riau, A., Yam, G., Halim, N. and Mehta, J.: Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy.
,Cells Vol. 11,, ,
, 2022 | |
| Cells: | Corneal stromal keratocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F-12 |
Eye: Human | |
| |
| Xu, N., Chen, Y., Dean, K., Lu, X., Liu, X., Wang, W., Dean, D., Kaplan, H., Gao, L., Dong, F. and Liu, Y.: Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation.
, Stem Cells 35,
1579-1591, 2017 | |
| Cells: | Muller glia |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Eye: Human | |
| |
| Sidney, L., Branch, M., Dua, H. and Hopkinson, A.: Effect of Culture Medium on Propagation and Phenotype of Corneal Stroma-Derived Stem Cells.
, Cytotherapy
17,
1706-22, 2015 | |
| Cells: | Corneal stroma stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Eye: Human | |
| |
| Du, Y., Roh, D., Funderburgh, M., Mann, M., Marra, K., Rubin, J., Li, X. and Funderburgh, J.: Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro.
, Mol Vis
16,
2680, 2010 | |
| Cells: | Corneal stromal stem |
| Enzyme(s) | Neutral Protease: 1.2 u/ml Collagenase: 0.1% |
| Medium | DMEM |
Eye: Human | |
| |
| Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells
, Inv Ophthal Visual Sci 48,
614-20, 2007 | |
| Cells: | Corneal endothelial |
| Enzyme(s) | Collagenase: 0.2% Neutral Protease: 1.0% |
| Medium | DMEM/F12 |
Eye: Human | |
| |
| Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet
, Inv Ophthal Visual Sci 44(10),
4275, 2003 | |
| Cells: | Corneal limbal epithelial sheet |
| Enzyme(s) | Neutral Protease: 5% |
| Medium | see reference |
Eye: Human | |
| |
| Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture
, Exp Eye Res 69,
97, 1999 | |
| Cells: | Retinal pigment epithelial (RPE) |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS |
Eye: Monkey | |
| |
| Han ,Y., Jacoby, R. and Wu, S.: Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells.
, Brain Res 875,
175, 2000 | |
| Cells: | Retinal |
| Enzyme(s) | Papain: 20-40 u/ml Deoxyribonuclease I: 400 u/ml |
| Medium | Ames' solution |
Eye: Monkey | |
| |
| Whittum-Hudson JA, Taylor HR: Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection
, Infect Immun 57,
2977, 1989 | |
| Cells: | Conjunctival lymphocytes |
| Enzyme(s) | Collagenase Type 1: 0.02% |
| Medium | RPMI 1640 |
Eye: Mouse | |
| |
| Liu, X., Tang, L. and Liu Y.: Mouse Muller Cell Isolation and Culture.
, Bio-protocol
7,
, 2017 | |
| Cells: | Muller |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Eye: Mouse | |
| |
| Ortin-Martinez, A., Tsai, E., Nickerson, P., Bergeret, M., Lu, Y., Smiley, S., Comanita, L. and Wallace, V.: A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors.
, Stem Cells 35,
932-939, 2017 | |
| Cells: | Retinal |
| Enzyme(s) | Papain: 20 u/ml Deoxyribonuclease I: 0.005% |
| Medium | PBS |
Eye: Mouse | |
| |
| Wohl, S., Jorstad, N., Levine, E. and Reh, T.: Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture.
, Nat Commun
8,
1603, 2017 | |
| Cells: | Retinal |
| Enzyme(s) | Papain: 20 u/ml Deoxyribonuclease I: 0.005% |
| Medium | HBSS |
Eye: Mouse | |
| |
| Blandford, S. and Baldridge, W.: The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i.
, Biomed Res Int
2016,
8178162, 2016 | |
| Cells: | Retinal astrocytes |
| Enzyme(s) | Papain: 16.5 u/ml Deoxyribonuclease I: 0.004% |
| Medium | HBSS |
Eye: Mouse | |
| |
| Feodorova, Y., Koch, M., Bultman, S., Michalakis, S. and Solovei, I.: Quick and Reliable Method for Retina Dissociation and Separation of Rod Photoreceptor Perikarya from Adult Mice.
, MethodsX
2,
39, 2015 | |
| Cells: | Retinal photoreceptor |
| Enzyme(s) | PDS kit: with modifications |
| Medium | EBSS |
Eye: Mouse | |
| |
| Balmer, J., Zulliger, R., Roberti, S. and Enzmann, V.: Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways.
, Int J Mol Sci 16,
15086-103, 2015 | |
| Cells: | Photoreceptors |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal |
Eye: Mouse | |
| |
| Dvoriantchikova, G., Perea-Martinez, I., Pappas, S., Barry, A., Danek, D., Dvoriantchikova, X., Pelaez, D. and Ivanov, D.: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina.
, PLoS ONE
10,
e0131054, 2015 | |
| Cells: | Retinal |
| Enzyme(s) | Papain: 8 u/ml Deoxyribonuclease I: 124 u/ml |
| Medium | DPBS |
Eye: Mouse | |
| |
| Iqbal, N., Xu, L., Devitt, C. and Skapek, S.: Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development.
, Biotechniques
56,
239-49, 2014 | |
| Cells: | Vitreous cells |
| Enzyme(s) | Collagenase: 0.1% Hyaluronidase: 0.03% |
| Medium | DMEM |
Eye: Mouse | |
| |
| Singh, M., Issa, P, Butler, R., Martin, C., Lipinski, D., Sekaran, S., Barnard, A. and Maclaren, R.: Reversal of End-Stage Retinal Degeneration and Restoration of Visual Bunction by Photoreceptor Transplantation.
, Proc Natl Acad Sci U S A ,
, 2013 | |
| Cells: | Retinal |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Eye: Mouse | |
| |
| Schmidt, T. and Kofuji, P.: An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells.
, J Vis Exp
47,
2367, 2011 | |
| Cells: | Retinal ganglion |
| Enzyme(s) | CLSPA: 240 u/ml Hyaluronidase: 0.2% |
| Medium | Ames |
Eye: Mouse | |
| |
| Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R., Ali, R. and Sowden, J.: Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells.
, Curr Protoc Stem Cell Biol
Chapter 1,
Unit 1H.4, 2011 | |
| Cells: | Cilary epithelial |
| Enzyme(s) | PDS kit: per instructions |
| Medium | EBSS |
Eye: Mouse | |
| |
| Ebihara, L., Tong, J., Vertel, B., White, T. and Chen, T.: Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells.
, Inv Ophthal Visual Sci 52,
882-9, 2011 | |
| Cells: | Lens Fiber |
| Enzyme(s) | Collagenase Type 4: 0.125% Protease: 0.5% |
| Medium | PBS |
Eye: Mouse | |
| |
| Jiang, C. Klassen, H., Zhang, X. and Young, M.: Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina.
, Mol Vis
16,
983, 2010 | |
| Cells: | Retinal progenitor |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HBSS |
Eye: Mouse | |
| |
| Kobayashi, T., Yoshioka, R., Shiraishi, A. and Ohashi, Y.: New Technique for Culturing Corneal Epithelial Cells of Normal Mice.
, Mol Vis
15,
1589-93, 2009 | |
| Cells: | Corneal epithelial |
| Enzyme(s) | Neutral Protease: 1.5% Trypsin: 0.25% |
| Medium | DMEM/F12 |
Eye: Mouse | |
| |
| Amadi-Obi, A., Yu, C., Liu, X., Mahdi, R., Clarke, G., Nussenblatt, R, Gery, I., Lee, Y. and Egwuagu, C.: TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1.
, Nat Med 13,
711, 2007 | |
| Cells: | Retinal |
| Enzyme(s) | Collagenase: 0.1% Deoxyribonuclease I: 0.001% |
| Medium | RPMI |
Eye: Mouse | |
| |
| Jadhav AP, Cho SH and Cepko CL: Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property
, Proc Natl Acad Sci U S A 103,
18998, 2006 | |
| Cells: | Retinal |
| Enzyme(s) | Papain: 50 u/ml |
| Medium | DMEM/F-12 |
Eye: Mouse | |
| |
| Yoshida, S., Shimmura, S., Nagoshi, N., Fukuda, K., Matsuzaki, Y., Okano, H. and Tsubota, K.: Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea.
, Stem Cells 24,
2714-22, 2006 | |
| Cells: | Stromal |
| Enzyme(s) | Trypsin: 0.05% Collagenase: 78 u/ml Hyaluronidase: 38 u/ml |
| Medium | DMEM/F-12 |
Eye: Mouse | |
| |
| Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto: Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission
, J Neurosci 25,
566-76, 2005 | |
| Cells: | Mouse retinal and bipolar |
| Enzyme(s) | Papain: 20 u/ml Deoxyribonuclease I: 200 u/ml |
| Medium | HBSS |
Eye: Mouse | |
| |
| Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells
, Mol Vis
9,
171, 2003 | |
| Cells: | Retinal endothelial cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Eye: Porcine | |
| |
| Xu, N., Chen, Y., Dean, K., Lu, X., Liu, X., Wang, W., Dean, D., Kaplan, H., Gao, L., Dong, F. and Liu, Y.: Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation.
, Stem Cells 35,
1579-1591, 2017 | |
| Cells: | Muller glia |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Eye: Porcine | |
| |
| Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells
, Acta Opthalmol Scand 81(2),
170, 2003 | |
| Cells: | Retinal pigment epithelial cells |
| Enzyme(s) | Collagenase: 2% |
| Medium | DMEM |
Eye: Rabbit | |
| |
| Lai, J., Cheng, H. and Ma, D.: Investigation of Overrun-Processed Porous Hyaluronic Acid Carriers in Corneal Endothelial Tissue Engineering
, PLoS ONE
10,
1371, 2015 | |
| Cells: | Corneal endothelial |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | HBSS |
Eye: Rabbit | |
| |
| Hao, M., Flynn, K., Nien-Shy, C., Jester, B., Winkler, M., Brown, D., La Schiazza, O., Bille, J., Jester, J.: In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelbert Two-Photon Laser Opthalmoscope
, Exp Eye Res 91,
308, 2010 | |
| Cells: | Corneal keratocytes |
| Enzyme(s) | Hyaluronidase: 0.05% Collagenase: 0.2% |
| Medium | DMEM |
Eye: Rabbit | |
| |
| Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C: Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes
, Inv Ophthal Visual Sci 45,
807-12, 2004 | |
| Cells: | Corneal keratocytes |
| Enzyme(s) | Trypsin: 0.25% Collagenase: 0.5% |
| Medium | PBS |
Eye: Rabbit | |
| |
| Brockway LM, Zhou Z-H, Bubien JK, Jovov B, Benos DJ, Keyser KT: Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits
, Am J Physiol Cell Physiol
283,
C126-34, 2002 | |
| Cells: | Retinal neurons |
| Enzyme(s) | Papain: 26 u/ml |
| Medium | DMEM |
Eye: Rabbit | |
| |
| Johnson-Muller, B., and Gross, J.: Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions
, Proc Natl Acad Sci U S A 75 (9),
4417, 1978 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS/DMEM |
Eye: Rat | |
| |
| Mead, B. and Tomarev, S.: Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms.
, Stem Cells Transl Med 6,
1273-1285, 2017 | |
| Cells: | Retinal |
| Enzyme(s) | PDS kit: with modifications |
| Medium | EBSS |
Eye: Rat | |
| |
| Cameron, M., Kekesi, O., Morley, J., Tapson, J., Breen, P., Van Schaik, A and Buskila, Y.: Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue.
, PLoS ONE
11,
e0155468, 2016 | |
| Cells: | Retinal |
| Enzyme(s) | Deoxyribonuclease I: 0.005% Papain: 30 u/ml |
| Medium | EBSS |
Eye: Rat | |
| |
| Vigneswara, V., Esmaeili, M., Deer, L., Berry, M., Logan, A. and Ahmed, Z.: Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration.
, Mol Cell Neurosci 68,
212-21, 2015 | |
| Cells: | Retinal |
| Enzyme(s) | PDS kit: per instructions |
| Medium | EBSS |
Eye: Rat | |
| |
| Tan, C., Andrews, M., Kwok, J., Heintz, T., Gumy, L., Fassler, R. and Fawcett, J.: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration.
, J Neurosci 32,
7325, 2012 | |
| Cells: | Retinal ganglion |
| Enzyme(s) | PDS kit: with modifications |
| Medium | Neurobasal |
Eye: Rat | |
| |
| Ma, J., Yu, W., Wang, Y., Cao, G., Cai, S., Chen, X., Yan, N., Yuan, Y., Zeng, H., Fleenor, D., Liu, X. and Pang, I.: Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells.
, Inv Ophthal Visual Sci 51,
3544-53, 2010 | |
| Cells: | Retinal ganglion |
| Enzyme(s) | Papain: 0.2% |
| Medium | Neurobasal |
Eye: Rat | |
| |
| Suzuki, T., Mandai, M., Akimoto, M., Yoshimura, N. and Takahashi, M.: The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina.
, Stem Cells 24,
2406, 2006 | |
| Cells: | Retinal |
| Enzyme(s) | PDS kit: per instructions |
| Medium | MEM |
Eye: Rat | |
| |
| Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.: Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue
, Inv Ophthal Visual Sci 45,
4570, 2004 | |
| Cells: | Ciliary-derived eye |
| Enzyme(s) | PDS kit: with modifications |
| Medium | DMEM/F12 |
Eye: Rat | |
| |
| Seigel, G.: The Golden Age of Retinal Cell Culture
, Mol Vis
5,
4, 1999 | |
| Cells: | Retinal |
| Enzyme(s) | Trypsin: 0.6% |
| Medium | DMEM |
Eye: Rat | |
| |
| Jing, S., Wen, D., Yu, Y., Holst, P., Luo, Y., Fang, M., Tamir, R., Antonio, L., Hu, Z., et al.: GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF
, Cell
85,
1113, 1996 | |
| Cells: | Retina |
| Enzyme(s) | Papain: 120 U |
| Medium | HBSS, PBS |
Eye: Rat | |
| |
| Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats
, Inv Ophthal Visual Sci 34(1),
101, 1993 | |
| Cells: | Retinal pigment epithelial cells |
| Enzyme(s) | Collagenase Type 1: 65 u/ml Hyaluronidase: 220 u/ml |
| Medium | CF Hanks with EDTA |
Eye: Rat | |
| |
| Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina
, Curr Eye Res 10(11),
1081, 1991 | |
| Cells: | Retinal pigment epithelial |
| Enzyme(s) | Neutral Protease: 2% |
| Medium | DMEM |
Eye: Rat | |
| |
| Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats
, J Neurosci 6,
3044, 1986 | |
| Cells: | Neurons, visual cortex |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | BSS (see reference) |
Eye: Rat | |
| |
| Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat
, J Neurosci 3 (12),
2532, 1983 | |
| Cells: | Retina |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Ham's F-12 |
Eye: Salamander | |
| |
| Clarke, R, Wang, J. and Townes-Anderson, E.: Using Laser Tweezers For Manipulating Isolated Neurons In Vitro.
, J Vis Exp
19,
911, 2008 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 14 u/ml |
| Medium | Ringers |
Eye: Salamander | |
| |
| Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase
, J Cell Biol
100,
175, 1985 | |
| Cells: | Retina |
| Enzyme(s) | Papain: 14 u/ml |
| Medium | Saline |
Eye: Salamander | |
| |
| Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina
, Proc Natl Acad Sci U S A 75,
3507, 1978 | |
| Cells: | Photoreceptors, retina |
| Enzyme(s) | Papain: 0.05% |
| Medium | (see reference) |
Eye: Turtle | |
| |
| Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors
, Proc Natl Acad Sci U S A 69,
1987, 1972 | |
| Cells: | Retinal |
| Enzyme(s) | Papain: 0.1% (13.5 u/mg) |
| Medium | Kreb's Ringer |
Heart: Bovine | |
| |
| Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons
, In Vitro Cell Dev Biol 26,
335, 1990 | |
| Cells: | Heart Adrenal chromaffin Paraneurons |
| Enzyme(s) | Trypsin: 0.06% |
| Medium | 25mM HEPES buffered Locke's solution, CMF |
Heart: Canine | |
| |
| Zhang,D., Wu, C., Qi, X., Meijering, R., Hoogstra-Berends, F., Tadevosyan, A., Cubukcuoglu, et al: Activation of Histone Deacetylase-6 Induces Contractile Dysfunction Through Derailment of alpha-tubulin Proteostasis in Experimental and Human Atrial Fibrillation.
, Circulation 129,
346-58, 2014 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 150 u/ml |
| Medium | Tyrode's solution |
Heart: Canine | |
| |
| Bonilla, I., Sridhar, A., Nishijima, Y., Gyorke, S., Cardounel, A. and Carnes, C.: Differential Effects of the Peroxynitrite Donor, SIN-1, on Atrial and Ventricular Myocyte Electrophysiology.
, J Cardiovasc Pharmacol 61,
401-7, 2013 | |
| Cells: | Atrial and ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.065% |
| Medium | See reference |
Heart: Canine | |
| |
| Xi, Y., Wu, G., Ai, T., Cheng, N., Kalisnik, J., Sun, J., Abbasi, S., Yang, D., Fan, C., Yuan, X., Wang, S. et al: Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium.
, Circ Arrhythm Electrophysiol
6,
976-83, 2013 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 300 u/ml Protease: 0.03% |
| Medium | Tyrode's solution |
Heart: Canine | |
| |
| Gan, T., Qiao, W., Xu, G., Zhou, X., Tang, B., Song, J., Li, Y., Zhang, J., Li, F., Mao, T. and Jiang, T.: Aging-Associated Changes in L-type Calcium Channels in the Left Atria of Dogs.
, Exp Ther Med
6,
919-924, 2013 | |
| Cells: | Atrial myocytes |
| Enzyme(s) | Collagenase Type 2: 0.03% |
| Medium | Tyrode's solution |
Heart: Canine | |
| |
| Harleton, E., Besana, A., Comas, G., Danilo, P., Rosen, T., Argenziano, M., Rosen, M., Robinson, R. and Feinmark, S.: Ability to Induce Atrial Fibrillation in the Peri-Operative Period is Associated with Phosphorylation-Dependent Inhibition of TWIK Protein-Related Acid-Sensitive Potassium Channel 1 (TASK-1).
, J Biol Chem 288,
2829-38, 2013 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 60 u/ml Protease XIV: 0.5 u/ml |
| Medium | See reference |
Heart: Canine | |
| |
| Zhang, H., Silva, J., Lin, Y., Verbsky, J., Lee, U., Kanter, E., Yamada, K., Schuessler, R. and Nichols, C.: Heterogeneity and Function of K(ATP) Channels in Canine Hearts.
, Heart Rhythm
10,
1576-83, 2013 | |
| Cells: | Atrial and ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.05-0.08% |
| Medium | See reference |
Heart: Canine | |
| |
| Calloe, K., Nof, E., Jespersen, T., Di Diego, J., Chlus, N., Olesen, S.¸ Antzelevitch, C. and Cordeiro, J.: Comparison of the Effects of a Transient Outward Potassium Channel Activator on Currents Recorded from Atrial and Ventricular Cardiomyocytes.
, J Cardiovasc Electrophysiol 22,
1057-66, 2011 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% Protease Type XIV: 0.01% |
| Medium | HEPES |
Heart: Canine | |
| |
| Burashnikov, A., Zygmunt, A., Di Diego, J., Linhardt, G., Carlsson, L. and Antzelevitch, C.: AZD1305 Exerts Atrial Predominant Electrophysiological Actions and is Effective in Suppressing Atrial Fibrillation and Preventing its Reinduction in the Dog.
, J Cardiovasc Pharmacol 56,
80-90, 2010 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | Tyrode's solution |
Heart: Canine | |
| |
| Gavi, S., Yin, D., Shumay, E., Wang, H. and Malbon, C.: Insulin-Like Growth Factor-I Provokes Functional Antagonism and Internalization of Beta1-Adrenergic Receptors.
, Endocrinology 148,
2653-62, 2007 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% Protease: 0.008% |
| Medium | M199 |
Heart: Canine | |
| |
| Schotten, U., de Haan, S., Verheule, S., Harks, E., Frechen, D., Bodewig, E., Greiser, M., Ram, R., Maessen, J., Kelm, M., Allessie, M. and Van Wagoner, D.: Blockade of Atrial-Specific K+-currents Increases Atrial but not Ventricular Contractility by Enhancing Reverse Mode Na+/Ca2+-Exchange.
, Cardiovasc Res 73,
37-47, 2007 | |
| Cells: | Atrial and ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.065% |
| Medium | Tyrode's solution |
Heart: Canine | |
| |
| Baba, S., Dun, W., Hirose, M. and Boyden, P.: Sodium Current Function in Adult and Aged Canine Atrial Cells.
, Am J Physiol Heart Circ Physiol
291,
H756-61, 2006 | |
| Cells: | Atrial myocytes |
| Enzyme(s) | Collagenase Type 2: 0.013% |
| Medium | HEPES |
Heart: Canine | |
| |
| Sun, H., Chartier, D., Leblanc, N. and Nattel, S.: Intracellular Calcium Changes and Tachycardia-Induced Contractile Dysfunction in Canine Atrial Myocytes.
, Cardiovasc Res 49,
751-61, 2001 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 110 u/ml |
| Medium | Tyrode's solution |
Heart: Canine | |
| |
| Gintant, G.: Two Components of Delayed Rectifier Current in Canine Atrium and Ventricle. Does IKs Play a Role in the Reverse Rate Dependence of Class III Agents?
, Circ Res 78,
26-37, 1996 | |
| Cells: | Ventricular and atrial myocytes |
| Enzyme(s) | Collagenase Type 4: 0.04% Collagenase Type 2: 125 u/ml |
| Medium | HEPES/Tyrode's |
Heart: Canine | |
| |
| Spanier, A. and Weglicki, W.: Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis
, Am J Physiol 243,
H448, 1982 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Joklik's MEM |
Heart: Chicken | |
| |
| Blech-Hermoni, Y. and Ladd, A.: Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq.
, Genom Data
6,
74-76, 2015 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.13% Collagenase Type 2: 0.13% Deoxyribonuclease I: 0.033% |
| Medium | HBSS |
Heart: Chicken | |
| |
| Eschenhagen, T., Fink, C., Remmers, U., Scholz, H., Wattchow, J., Weil, J., et al.: Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System
, FASEB J
11,
683, 1997 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS |
Heart: Chicken | |
| |
| Wang, S., Greaser, M.L., Schultz, E., Bulinski, J.C., Lin, J.J., and Lessard, J.L.: Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin
, J Cell Biol
107,
1075, 1988 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.17% |
| Medium | HBSS, CMF |
Heart: Chicken | |
| |
| Kim, D., Okada, A., and Smith, T.W.: Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells
, Circ Res 61,
29, 1987 | |
| Cells: | Ventricular |
| Enzyme(s) | Trypsin: 0.025% |
| Medium | HBSS, CMF |
Heart: Chicken | |
| |
| Jacob, R., Lieberman, M., Murphy, E., and Piwnica-Worms, D.: Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells
, J Physiol
387,
549, 1987 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HBSS, CMF |
Heart: Chicken | |
| |
| Murphy, E., Aiton, J., Russell, C., and Lieberman, M.: Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury
, Am J Physiol Cell Physiol
245 (14),
C316, 1983 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 0.025% |
| Medium | CMF solution |
Heart: Chicken | |
| |
| Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells
, Exp Cell Res
53,
1, 1968 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin NF 1:250: 0.25% |
| Medium | Rinaldini's buffer solution, CF |
Heart: Chicken | |
| |
| Dehann, R.: Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture
, Dev Biol 16,
216, 1967 | |
| Cells: | Heart |
| Enzyme(s) | Trypsin: 0.025% |
| Medium | Medium 199 |
Heart: Chicken | |
| |
| Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation
, Exp Cell Res
30,
257, 1963 | |
| Cells: | Heart Liver |
| Enzyme(s) | Trypsin: 3.0% |
| Medium | Tyrode's solution, CMF |
Heart: Feline | |
| |
| Silver, L., Hemwall, E., Marino, T., and Houser, S.: Isolation and Morphology of Calcium-Tolerant Feline Ventricular Myocytes
, Am J Physiol 245,
H891, 1983 | |
| Cells: | Myocytes Ventricular |
| Enzyme(s) | Collagenase Type 2: 0.12% |
| Medium | Kreb's Henseleit, CF |
Heart: Fish | |
| |
| Fowler, E., Wang, N., Hezzell, M., Chanoit, G., Hancox, J. and Cannell, M.: Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration
, Proc Natl Acad Sci U S A 117,
2687-2692, 2020 | |
| Cells: | Cardiac Myocytes |
| Enzyme(s) | Protease XIV: 0.005% Collagenase Type 1: 0.1-0.2% Trypsin: 0.08% |
| Medium | HEPES |
Heart: Fish | |
| |
| Sander, V., Sune, G., Jopling, C., Morera, C. and Belmonte J.: Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts.
, Nat Protoc
8,
800-9, 2013 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.5% Collagenase Type 4: 0.5% |
| Medium | MEM |
Heart: Frog | |
| |
| Arrio-Dupont, M., and de Nay, D.: High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles, Biol Cell 54,
164, 1985 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 0.04% |
| Medium | CF Ringer |
Heart: Guinea-Pig | |
| |
| Wang, Y., Geng, J., Jiang, M., Li, C., Han, Y and Jiang, J.: The Cardiac Electrophysiology Effects of Higenamine in Guinea Pig Heart
, Biomed Pharmacother 109,
2348, 2019 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.4% |
| Medium | Tyrodes solution |
Heart: Guinea-Pig | |
| |
| Zorn-Pauly, K., Schaffer, P., Pelzmann, B., Bernhart, E., Lang, P., and Koidl, B.: L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes
, Physiol Res 53(4),
369, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 100 u/ml |
| Medium | M-199 |
Heart: Guinea-Pig | |
| |
| Dhamoon, A, Pandit, S., Sarmast, F., Parisian, K., Guha, P., Li, Y., Bagwe, S., Taffet, S. and Anumonwo, J.: Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current.
, Circ Res 94,
1332-9, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 100-200 u/ml |
| Medium | See reference |
Heart: Guinea-Pig | |
| |
| Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium
, Am J Physiol Heart Circ Physiol
281,
H1657-66, 2001 | |
| Cells: | Coronary endothelial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | see reference |
Heart: Guinea-Pig | |
| |
| Preisig-Muller, R., Mederos Y Schnitzler, M., Derst, C., and Daut J.: Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR
, Am J Physiol 277,
H413, 1999 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | Perfusing solution (see reference) |
Heart: Guinea-Pig | |
| |
| Stemmer, P., Akera,T., Brody, T., Rardon, D., and Watanabe, A.: Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart
, Life Sci 44,
1231, 1989 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.02% |
| Medium | Krebs Henseleit bicarbonate buffer |
Heart: Guinea-Pig | |
| |
| Ishihara, K., Mitsuiye, T., Noma, A., and Takano, M.: The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes
, J Physiol
419,
297, 1989 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase: 0.04% |
| Medium | Tyrode solution, CF |
Heart: Guinea-Pig | |
| |
| Bridge, J., Spitzer, K., and Ershler, P.: Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent
, Science 241,
823, 1988 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.10% |
| Medium | Bicarbonate buffer, CF |
Heart: Human | |
| |
| Holt-Casper, D., Theisen, J., Moreno, A., Warren, M., Silva, F., Grainger, D., Bull, D. and Patel, A.: Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds.
, J Transl Med
13,
194, 2015 | |
| Cells: | Cardiac |
| Enzyme(s) | Collagenase: 0.045% Pancreatin: 0.1% |
| Medium | DPBS |
Heart: Human | |
| |
| Sharma, S., Mishra, R., Simpson, D., Wehman, B., Colletti, E., Deshmukh, S., Datla, S., Balachandran, K., Guo, Y., Chen, L., Siddiqui, O., Kaushal, S. and Kaushal, S.: Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome.
, Stem Cells 33,
1213-29, 2015 | |
| Cells: | Cardiospheres |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 0.08% Trypsin: 0.05% |
| Medium | Iscove modified DMEM |
Heart: Human | |
| |
| Avolio, E., Gianfranceschi, G., Cesselli, D., Caragnano, A., Athanasakis, E., Katare, R., Meloni, M., Palma, A., Barchiesi, A., Vascotto, C., Toffoletto, B., Mazzega, E., Finato, N., Aresu, G. and Livi, U.: Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction.
, Stem Cells 32,
2373, 2014 | |
| Cells: | Cardiac stem |
| Enzyme(s) | Collagenase Type 2: 0.25% |
| Medium | Joklik modified Eagle’s |
Heart: Human | |
| |
| Voigt, N., Zhou, X. and Dobrev, D.: Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents.
, J Vis Exp
,
e50235, 2013 | |
| Cells: | Atrial myocytes |
| Enzyme(s) | Collagenase Type 1: 286 u/ml Protease: 5 u/ml |
| Medium | see references |
Heart: Human | |
| |
| Vukusic, K., Jonsson, M., Brantsing, C., Dellgren, G., Jeppsson, A., Lindahl, A. and Asp, J.: High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis.
, Biomed Res Int
2013,
, 2013 | |
| Cells: | Cardiac |
| Enzyme(s) | Collagenase Type 2: 0.08% |
| Medium | DMEM |
Heart: Human | |
| |
| Anderson, E., Rodriguez, E., Anderson, C., Thayne, K., Chitwood, W. and Kypson, A.: Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways.
, Am J Physiol Heart Circ Physiol
300,
H118, 2011 | |
| Cells: | Myofibers |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | see reference |
Heart: Human | |
| |
| Voigt, N., Makary, S., Nattel, S. and Dobrev, D.: Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart.
, Methods Enzymol
484,
653, 2010 | |
| Cells: | Atrial cardiomyocytes |
| Enzyme(s) | Collagenase Type 1: 286 u/ml Protease: 5 u/ml |
| Medium | see reference |
Heart: Human | |
| |
| Jensen, B., Swigart, P., Laden, M., DeMarco, T., Hoopes, C. and Simpson, P.: The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries.
, J Am Coll Cardiol
54,
1137, 2009 | |
| Cells: | Coronary artery smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.1% Elastase: 0.05% |
| Medium | DMEM |
Heart: Human | |
| |
| Mathew, S., Tustison, K., Sugatani, T., Chaudhary, L., Rifas, L. and Hruska, K.: The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD.
, J Am Soc Nephrol
19,
1092, 2008 | |
| Cells: | Smooth muscle aortic |
| Enzyme(s) | Collagenase Type 1: 0.2% Collagenase Type 2: 0.1% Elastase: 0.025% |
| Medium | DMEM |
Heart: Human | |
| |
| Mukerjee, R., Multani, M., Sample, J., Dowdy, K., Zellner, J., Hoover, D., and Spinale, F.: Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response
, J Cardiovasc Pharmacol 7 (4),
235, 2002 | |
| Cells: | Myocardial |
| Enzyme(s) | Hyaluronidase: 0.05% |
| Medium | Medium 199 |
Heart: Human | |
| |
| Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N: Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes
, Am J Physiol Heart Circ Physiol
283,
H990-5, 2002 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% Collagenase Type 1: 0.025% Protease XIV: 0.013% |
| Medium | HEPES solution |
Heart: Human | |
| |
| Hoppe, U., Jansen, E., Sudkamp, M., and Beukelmann, D.: Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts
, Circulation 97,
55, 1998 | |
| Cells: | Heart Myocytes |
| Enzyme(s) | Collagenase Type 2: 200 u/ml |
| Medium | Tyrode's solution |
Heart: Human | |
| |
| Shirai, T., Rao, V., Weisel, R., Ikonomidis, J., Li, R., Tumiati, L., Merante, F. and Mickle, D.: Preconditioning Human Cardiomyocytes and Endothelial Cells.
, J Thorac Cardiovasc Surg 115,
210-9, 1998 | |
| Cells: | Cardiomyocytes and endothelial |
| Enzyme(s) | Trypsin: 0.2% Collagenase: 0.1% |
| Medium | PBS |
Heart: Human | |
| |
| Van Wagoner, D., Pond, A., McCarthy, P., Trimmer, J. and Nerbonne, J.: Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation.
, Circ Res 80,
772-81, 1997 | |
| Cells: | Atrial myocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% Protease Type XIV: 0.04% |
| Medium | see reference |
Heart: Human | |
| |
| Goldman, B., and Wurzel, J.: Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes
, In Vitro Cell Dev Biol 28,
109, 1992 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | HBSS with Calcium |
Heart: Human | |
| |
| Smith, D., Glover, J., Townsend, L., and Maupin, E.: A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor
, In Vitro Cell Dev Biol 27,
914, 1991 | |
| Cells: | Myocardial Atrial |
| Enzyme(s) | Collagenase: 0.14% |
| Medium | HBSS, CMF |
Heart: Human | |
| |
| Bugaisky, L.B.: Biology of Isolated Adult Cardiac Myocytes,Isolation and Culture of Human Adult Cardiac Myocytes,(Clark,Decker,Borg,eds), Elsevier Science Publishing Co., Inc.,
, 1988 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Joklik's MEM |
Heart: Human | |
| |
| Hassler, O., Wiren, M., and Herbertsson, S.: The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase, Acta Pathol Microbiol Scand 57,
15, 1963 | |
| Cells: | Thoracic aorta |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Phosphate buffer w/NaCl |
Heart: Invertebrate | |
| |
| Altimiras J., Hove-Madsen L., and Gesser H.: Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods
, J Exp Biol
202,
2531, 1999 | |
| Cells: | Systemic heart cardiomyocytes |
| Enzyme(s) | Collagenase: 0.025% Trypsin: 0.02% |
| Medium | see reference |
Heart: Mouse | |
| |
| Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R and Chen-Izu, Y: In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models.
, PLoS ONE
11,
e0160605, 2016 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 300 u/ml Protease XIV: 0.004% |
| Medium | Perfusion solution (see reference) |
Heart: Mouse | |
| |
| Hu, D., Yin, C., Mohanta, S., Weber, C. and Habenicht, A.: Prepartaion of Single Cell Suspensions from Mouse Aorta
, Bio Protoc
6,
11, 2016 | |
| Cells: | Aortic |
| Enzyme(s) | Collagenase Type 1: 400 u/ml Collagenase: 120 u/ml Hyaluronidase: 60 u/ml Deoxyribonuclease I: 60 u/ml |
| Medium | DPBS |
Heart: Mouse | |
| |
| Xu, J., Lee, Y., Ran, X., Liao, S., Yang, J., Au, K., Lai, W., Esteban, M., Tse, H.: Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration.
, Stem Cells 34,
2693-2706, 2016 | |
| Cells: | Cardiospheres |
| Enzyme(s) | Collagenase Type 2: 0.4% Neutral Protease: 0.4% |
| Medium | PBS |
Heart: Mouse | |
| |
| Henn, M., Janjua, M., Kanter, E., Makepeace, C., Schuessler, R., Nichols, C. and Lawton, J.: Adenosine Triphosphate-Sensitive Potassium Channel Kir Subunits Implicated in Cardioprotection by Diazoxide.
, J Am Heart Assoc
4,
, 2015 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.12% |
| Medium | see reference |
Heart: Mouse | |
| |
| Wang, H., Chen, H., Feng, B., Wang, X., He, X., Hu, R., Yin, M., Wang, W., Fu, W. and Xu, Z.: Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue.
, BMC Biotechnol
14,
75, 2014 | |
| Cells: | Cardiac progenitor |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Heart: Mouse | |
| |
| Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H and Pinto-d-O, P: Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms.
, Stem Cell Res Ther 5,
5, 2014 | |
| Cells: | Cardiac Progenitor |
| Enzyme(s) | Collagenase Type 2: 600 u/ml Deoxyribonuclease I: 60 u/ml |
| Medium | MEM |
Heart: Mouse | |
| |
| Zafiriou, M., Noack, C., Unsold, B., Didie, M., Pavlova, E., Fischer, H., Reichardt, H., Bergmann, M., El-Armouche, A., Zimmermann, W., Zelarayan, L.: Erythropoietin Responsive Cardiomyogenic Cells Contribute to Heart Repair Post Myocardial Infarction.
, Stem Cells 32,
2480, 2014 | |
| Cells: | Cardiac progenitor |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 2.4 u/ml |
| Medium | HBSS |
Heart: Mouse | |
| |
| Kohncke, C. Lisewski, U., Schleussner, L., Gaertner, C., Reichert, S. and Roepke, T.: Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes.
, J Vis Exp
73,
e50145, 2013 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.08% |
| Medium | see reference |
Heart: Mouse | |
| |
| Hennessey, J., Wei, E. and Pitt, G.: Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels.
, Circ Res 113,
381-8, 2013 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 150 u/ml |
| Medium | MEM |
Heart: Mouse | |
| |
| Touchberry, C., Green, T., Tchikrizov, V., Mannix, J., Mao, T., Carney, B., Girgis, M., Vincent, R., Wetmore, L., Dawn, B., Bonewald, L., Stubbs, J. and Wacker, M.: FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy.
, Am J Physiol Endocrinol Metab
304,
E863, 2013 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 360 u/ml Papain: 0.4 u/ml Deoxyribonuclease I: 40 u/ml |
| Medium | L-15 |
Heart: Mouse | |
| |
| Pratumvinit, B., Reesukumal, K., Janebodin, K., Ieronimakis, N. and Reyes, M.: Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells.
, Biomed Res Int
2013,
359412, 2013 | |
| Cells: | Cardiac endothelial |
| Enzyme(s) | Collagenase Type 4: 0.2% Neutral Protease: 1.2 u/ml |
| Medium | PBS |
Heart: Mouse | |
| |
| Christel, C., Cardona, N., Mesirca, P., Herrmann, S., Hofmann, F., Striessnig, J., Ludwig, A., Mangoni, M. and Lee, A.: Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node.
, J Physiol
590,
6327, 2012 | |
| Cells: | Sinoatrial node |
| Enzyme(s) | Collagenase Type 2: 229 u/ml Elastase: 1.9 u/ml Protease Type XIV: 0.9 u/ml |
| Medium | Tyrode's solution |
Heart: Mouse | |
| |
| Carley, A. and Kleinfeld, A.: Fatty Acid (FFA) Transport in Cardiomyocytes Revealed by Imaging Unbound FFA is Mediated by an FFA Pump Modulated by the CD36 Protein.
, J Biol Chem 286,
4589-97, 2011 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 59 u/ml |
| Medium | MEM |
Heart: Mouse | |
| |
| Butcher, M., Herre, M., Ley, K. and Galkina, E.: Flow Cytometry Analysis of Immune Cells Within Murine Aortas.
, J Vis Exp
53,
e2828, 2011 | |
| Cells: | Aortic adventitial leukocytes |
| Enzyme(s) | Collagenase Type 2: 300 u/ml Elastase: 5.6 u/ml |
| Medium | PBS |
Heart: Mouse | |
| |
| Flynn, J., Santana, L. and Melov, S.: Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes.
, J Vis Exp
58,
e3302, 2011 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 1: 0.1-0.4% Protease Type XIV: 0.004-.02% |
| Medium | see reference |
Heart: Mouse | |
| |
| Makino, A., Suarez, J., Wang, H., Belke, D., Scott, B. and Dillmann, W.: Thyroid Hormone Receptor-beta is Associated With Coronary Angiogenesis During Pathological Cardiac Hypertrophy.
, Endocrinology 150,
2008, 2009 | |
| Cells: | Coronary endothelial cells |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 0.6 u/ml |
| Medium | M199 |
Heart: Mouse | |
| |
| Zhang, Y, Kanter, E., Laing, J., Aprhys, C., Johns, D., Kardami, E. and Yamada, K.: Connexin43 Expression Levels Influence Intercellular Coupling and Cell Proliferation of Native Murine Cardiac Fibroblasts.
, Cell Commun Adhes
15,
289, 2008 | |
| Cells: | Ventricular myoctes |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | M199 |
Heart: Mouse | |
| |
| Kabaeva, Z., Zhao, M. and Michele, D.: Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression.
, Am J Physiol Heart Circ Physiol
294,
H1667-74, 2008 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 620 u/ml Protease XIV: 0.104 u/ml Deoxyribonuclease I: 0.0015% |
| Medium | Myocyte buffer see reference |
Heart: Mouse | |
| |
| O'Connell Timothy D, Rodrigo Manoj C, Simpson Paul C: Isolation and culture of adult mouse cardiac myocytes
, Methods Mol Biol 357,
271-96, 2007 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase: see reference |
| Medium | HBSS |
Heart: Mouse | |
| |
| Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy
, J Am Coll Cardiol
49,
706, 2007 | |
| Cells: | Smooth muscle cells |
| Enzyme(s) | Collagenase Type 2: 300 u/ml Elastase: 5 u/ml |
| Medium | F10 Ham |
Heart: Mouse | |
| |
| Shioya, T: A Simple Technique for Isolating Healthy Heart Cells from Mouse Models.
, J Physiol Sci
57,
327-35, 2007 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% Trypsin: 0.006% Protease XIV: 0.006% |
| Medium | see reference |
Heart: Mouse | |
| |
| Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms
, J Physiol
575,
627-44, 2006 | |
| Cells: | Ventricular myocytes and mesenteric arterial SMC |
| Enzyme(s) | Collagenase Type 2: 0.06% Papain: 0.175% |
| Medium | Krebs-Ringer |
Heart: Mouse | |
| |
| Chen, H., Yong, W., Ren, S., Shen, W., He, Y., Cox, K., Zhu, W., Li, W., Soonpaa, M., Payne, RM, Franco, D., Field, L., Rosen, V., Wang, Y. and Shou, W.: Overexpression of Bone Morphogenetic Protein 10 in Myocardium Disrupts Cardiac Postnatal Hypertrophic Growth.
, J Biol Chem 281,
27481, 2006 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 1: 0.17% |
| Medium | PBS |
Heart: Mouse | |
| |
| Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.: ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes
, Cell Physiol Biochem 15,
263, 2005 | |
| Cells: | Cardiomyoctes |
| Enzyme(s) | Collagenase Type 1: 150 u/ml Trypsin: 0.01% |
| Medium | M199 |
Heart: Mouse | |
| |
| Potts, Malia B., Vaughn, Allyson E., McDonough, Holly, Patterson, Cam, and Deshmukh, Mohanish: Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP
, J Cell Biol
171,
925, 2005 | |
| Cells: | Cardiomyoctes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | EBSS |
Heart: Mouse | |
| |
| Kobayashi, M., Inoue, K., Warabi, E., Minami, T. and Kodama, T.: A Simple Method of Isolating Mouse Aortic Endothelial Cells.
, J Atheroscler Thromb
12,
138, 2005 | |
| Cells: | Aortic endothelial |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Heart: Mouse | |
| |
| Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori: Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques
, Circulation 110,
1953-9, 2004 | |
| Cells: | Smooth muscle cells |
| Enzyme(s) | Collagenase Type 1: 0.1% Elastase: 0.0125% |
| Medium | DMEM |
Heart: Mouse | |
| |
| Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H: Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo
, Circulation 110,
3803-7, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.02% Elastase: 0.03% Pancreatin: 0.06% |
| Medium | see reference |
Heart: Mouse | |
| |
| Qian, Q., Hunter, L., Li, M., Marin-Padilla, M., Prakash, Y., Somlo, S., Harris, P., Torres, V., and Sieck, G.: Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells
, Hum Mol Genet
12(15),
1875, 2003 | |
| Cells: | Vascular smooth muscle |
| Enzyme(s) | Papain: 10 u/ml Elastase: .005% Collagenase: 0.05% Deoxyribonuclease I: 1000 u/ml PDS kit: with modifications |
| Medium | EBSS |
Heart: Mouse | |
| |
| Rybkin, I., Markham, D., Yan, Z., Bassel-Duby, R., Williams, R., and Olson, E.: Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation
, J Biol Chem 278(18),
15927, 2003 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% Pancreatin: 0.06% |
| Medium | DMEM/F-12 |
Heart: Mouse | |
| |
| Bettahi, I,, Marker, C., Roman, M., and Wickman, K.: Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh
, J Biol Chem 277,
48282, 2002 | |
| Cells: | Atrial myocytes |
| Enzyme(s) | NCIS kit: with modifications see reference |
| Medium | L-15 |
Heart: Mouse | |
| |
| Schreiber, K., Paquet, L., Allen, B., and Rindt, H.: Protein Kinase C Isoform Expression and Activity in the Mouse Heart
, Am J Physiol Heart Circ Physiol
281(5),
H2062, 2001 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.03-0.2% |
| Medium | Joklik's MEM |
Heart: Mouse | |
| |
| Nelson, D., Setser, E., Hall, D., Schwartz, S., Hewitt, T., Klevitsky, R., Osinska, H., Bellgrau, D., Duke, R., and Robbins, J.: Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart
, J Clin Invest 105 (9),
1199, 2000 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 150 u/ml |
| Medium | Joklik's MEM |
Heart: Mouse | |
| |
| Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G, and Coles JG.: Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture
, Transplantation 70,
1310, 2000 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% Deoxyribonuclease I: 0.002% |
| Medium | PBS |
Heart: Mouse | |
| |
| Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP: Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology
, Am J Physiol Heart Circ Physiol
279,
H429-36, 2000 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% Collagenase Type 4: 0.05% Protease XIV: 0.002% |
| Medium | MEM |
Heart: Mouse | |
| |
| Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR: High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates
, Circulation 101,
178-84, 2000 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 150 u/ml |
| Medium | Joklik's MEM |
Heart: Mouse | |
| |
| Wang, G., and Kang, Y.: Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels
, J Pharmacol Exp Ther 288(3),
938, 1999 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | FBS-MEM |
Heart: Mouse | |
| |
| Lader, A., Kwiatkowski, D., and Cantiello, H.: Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels
, Am J Physiol 277,
C1277, 1999 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: with modifications see reference |
| Medium | L-15 |
Heart: Mouse | |
| |
| Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation
, Mol Biol Cell
10,
1569-79, 1999 | |
| Cells: | Fibroblasts, mesangial, smooth muscle |
| Enzyme(s) | Trypsin: 0.25% Collagenase: see reference Soybean Trypsin Inhibitor: 0.05% |
| Medium | DMEM |
Heart: Mouse | |
| |
| Felzen, B., Shilkrut, M., Less, H., Sarapov, I., Maor, G., Coleman, R., Robinson, R., et al.: Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice
, Circ Res 82,
438, 1998 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.04% |
| Medium | Tyrode's solution |
Heart: Mouse | |
| |
| Lader, A., Xiao, Y., Ishikawa, Y., Cui, Y., Vatner, D., Vatner, S., Homcy, C., Cantiello, H.: Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway
, Proc Natl Acad Sci U S A 95,
9669, 1998 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: with modifications see reference |
| Medium | L-15 |
Heart: Mouse | |
| |
| Grupp, I., Lorenz, J., Walsh, R., Boivin, G. and Rindt, H.: Overexpression of Alpha1B-Adrenergic Receptor Induces Left Ventricular Dysfunction in the Absence of Hypertrophy.
, Am J Physiol 275,
H1338-50, 1998 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 1: 75 u/ml Collagenase Type 2: 75 u/ml |
| Medium | Joklik's MEM |
Heart: Mouse | |
| |
| Arber, S., Hunter, J., Ross, J., Hongo, M., Sansig, G., Borg, J., Perriard, J., Chien, K., and Caroni, P.: MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure
, Cell
88,
393, 1997 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase: 0.045% |
| Medium | DMEM, Medium 199 |
Heart: Mouse | |
| |
| Valenzuela, D., Han, X., Mende, U., Fankhauser, C., Mashimo, H., Huang, P., Pfeffer, J., Neer, E., and Fishman, M.: G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart
, Proc Natl Acad Sci U S A 94(5),
1727, 1997 | |
| Cells: | Ventricular and atrial myocytes |
| Enzyme(s) | Collagenase Type 2: 95 u/ml Hyaluronidase: 172.5 u/ml Trypsin: 0.002% Deoxyribonuclease I: 60u/ml |
| Medium | DMEM/Tyrodes |
Heart: Mouse | |
| |
| Soonpaa, M., Kim, K., Pajak, aL., Franklin, M., and Field, L.: Cardiomyocyte DNA Synthesis and Binucleation During Murine Development
, Am J Physiol 271,
H2183, 1996 | |
| Cells: | Cardiomyocyte |
| Enzyme(s) | Collagenase: 0.17 % |
| Medium | PBS |
Heart: Mouse | |
| |
| Wobus, A., Kleppisch, T., Maltsev, V., and Hescheler, J.: Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels
, In Vitro Cell Dev Biol 30A,
425, 1994 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Heart: Mouse | |
| |
| Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells
, Exp Cell Res
53,
1, 1968 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin NF 1:250: 0.25% |
| Medium | Rinaldini's buffer solution, CF |
Heart: Ovine | |
| |
| Voigt, N., Pearman, C., Dobrev, D. and Dibb, K.: Methods for Isolating Atrial Cells from Large Mammals and Humans.
, J Mol Cell Cardiol 86,
187-98, 2015 | |
| Cells: | Atrial myocytes |
| Enzyme(s) | Collagenase Type 2: 0.055% Protease XIV: 0.006% |
| Medium | Tyrode's solution |
Heart: Ovine | |
| |
| Dibb, K., Clarke, J., Horn, M., Richards, M., Graham, H., Eisner, D. and Trafford, A.: Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure.
, Circ Heart Fail
2,
482-9, 2009 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.055% Protease Type XIV: 0.006% |
| Medium | See reference |
Heart: Ovine | |
| |
| Dhamoon, A, Pandit, S., Sarmast, F., Parisian, K., Guha, P., Li, Y., Bagwe, S., Taffet, S. and Anumonwo, J.: Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current.
, Circ Res 94,
1332-9, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 100-200 u/ml |
| Medium | See reference |
Heart: Porcine | |
| |
| Butcher, J. and Nerem, R.: Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells.
, J Heart Valve Dis 13,
478, 2004 | |
| Cells: | Aortic smooth muscle and intersitial cells |
| Enzyme(s) | Collagenase: 600 u/ml |
| Medium | PBS |
Heart: Porcine | |
| |
| Christ, M., Gunther, A., Heck, M., Schmidt, B., Falkenstein, E., and Wehling, M.: Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells
, Circulation 99,
1485, 1999 | |
| Cells: | Coronary vascular smooth muscle (PCVSMCs) |
| Enzyme(s) | Trypsin: 0.037% |
| Medium | HEPES |
Heart: Porcine | |
| |
| Bowles, D., Hu, Q., Laughlin, M., and Sturek, M.: Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle
, Am J Physiol 275 (44),
H2159, 1998 | |
| Cells: | Coronary smooth muscle |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Krebs bicarbonate solution |
Heart: Rabbit | |
| |
| Fowler, E., Wang, N., Hezzell, M., Chanoit, G., Hancox, J. and Cannell, M.: Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration
, Proc Natl Acad Sci U S A 117,
2687-2692, 2020 | |
| Cells: | Cardiac Myocytes |
| Enzyme(s) | Protease XIV: 0.005% Collagenase Type 1: 0.1-0.2% Trypsin: 0.08% |
| Medium | HEPES |
Heart: Rabbit | |
| |
| Farkas, A., Acsai, K., Nagy, N., Toth, A., Fulop, F., Seprenyi, G., Birinyi, P., Nanasi, P., Forster, T., Csanady, M., Papp, J., Varrro, A. and Farkas, A.: Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart.
, Br J Pharmacol 154,
93, 2008 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 1: 0.05% Hyaluronidase: see reference Protease: see reference |
| Medium | Krebs-Henseleit |
Heart: Rabbit | |
| |
| Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy
, J Am Coll Cardiol
49,
706, 2007 | |
| Cells: | Smooth muscle cells |
| Enzyme(s) | Collagenase Type 2: 300 u/ml Elastase: 5 u/ml |
| Medium | F10 Ham |
Heart: Rabbit | |
| |
| Driesen Ronald B, Dispersyn Gerrit D, Verheyen Fons K, van den Eijnde Stefan M, Hofstra Leo, Thone Fred, Dijkstra Petra, Debie Wiel, Borgers Marcel, Ramaekers Frans C S: Partial cell fusion: a newly recognized type of communication between dedifferentiating cardiomyocytes and fibroblasts
, Cardiovasc Res 68,
37-46, 2005 | |
| Cells: | Cardiomyocytes and fibroblasts |
| Enzyme(s) | Collagenase: 0.06% |
| Medium | Medium 199 |
Heart: Rabbit | |
| |
| Spitzer, K., Ershler, P., Skolnick, R., and Vaughan-Jones, R.: Generation of Intracellular pH Gradients in Single Cardiac Myocytes with a Microperfusion System
, Am J Physiol 278,
H1371, 2000 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | HEPES |
Heart: Rabbit | |
| |
| Sedarat, F., Xu, L., Moore, E., and Tibbits, G.: Colocalization of Dihydropyridine and Ryanodine Receptors in Neonate Rabbit Heart Using Confocal Microscopy
, Am J Physiol Heart Circ Physiol
279,
H202, 2000 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | EGTA-KB |
Heart: Rabbit | |
| |
| Anumonwo JM, Delmar M, and Jalife J.: Electrophysiology of single heart cells from the rabbit tricuspid valve
, J Physiol
425,
145, 1990 | |
| Cells: | Tricuspid valve cells |
| Enzyme(s) | Collagenase: 0.8 mg/ml |
| Medium | HEPES Tyrode solution |
Heart: Rabbit | |
| |
| Buxton, I., and Brunton, L.: Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes
, J Biol Chem 258 (17),
10233, 1983 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Hyaluronidase: 0.5% |
| Medium | Eagle's MEM |
Heart: Rabbit | |
| |
| Dani, A., Cittadini, A., Flamini, G., Festuccia, G., and Terranova, T.: Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart
, J Mol Cell Cardiol 9,
777, 1977 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.007% |
| Medium | Kreb's Ringer |
Heart: Rat | |
| |
| Zhang, Y., Wang, Y., Yanni, J., Qureshi, M., Logantha, S., Kassab, S., Boyett, M., Gardiner, N., Sun, H., Howarth, F. and Dobrzynski, H.: Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart.
, Front Physiol
10,
826, 2019 | |
| Cells: | Sinoatrial nodal |
| Enzyme(s) | Collagenase Type 4: 220 u/ml Elastase: 1.4 u/ml Protease: 0.45 u/ml |
| Medium | Tyrode solution |
Heart: Rat | |
| |
| Savi, M., Bocchi, L., Mena, P., Dall'Asta, M., Crozier, A., Brighenti, F., Stilli, D. and Del Rio, D.: In vivo Administration of Urolithin A and B Prevents the Occurrence of Cardiac Dysfunction in Streptozotocin-induced Diabetic Rats.
, Cardiovasc Diabetol
16,
80, 2017 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% Protease Type XIV: 0.01% |
| Medium | Tyrode solution |
Heart: Rat | |
| |
| Abdul-Ghani, M., Suen, C., Jiang, B., Deng, Y., Weldrick, J., Putinski, C., Brunette, S., Fernando, P., Lee, T., Flynn, P., Leenen, F., Burgon, P. Stewart, D. and Megeney, L.: Cardiotrophin 1 Stimulates Beneficial Myogenic and Vascular Remodeling of the Heart.
, Cell Res
27,
1195-1215, 2017 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 200 u/ml |
| Medium | DMEM |
Heart: Rat | |
| |
| Stoppel, W., Gao, A., Greaney, A., Partlow, B., Bretherton, R., Kaplan, D and Black, L: Elastic, Silk-Cardiac Extracellular Matrix Hydrogels Exhibit Time-Dependent Stiffening that Modulates Cardiac Fibroblast Response.
, J Biomed Mater Res A
104,
3058, 2016 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Collagenase Type 2: 300 u/ml |
| Medium | DMEM |
Heart: Rat | |
| |
| Xie, L., Pi, X., Townley-Tilson, WH., Li, N., Wehrens, X., Entman, M., Taffet, G., Mishra, A., Peng, J., Schisler, J., Meissner, G. and Patterson, C.: PHD2/3-dependent Hydroxylation Tunes Cardiac Response to β-adrenergic Stress via Phospholamban.
, J Clin Invest 125,
2759-71, 2015 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | MEM |
Heart: Rat | |
| |
| Calderon-Sachez, E., Dominguez-Rodriguez, A., Lopez-Haldon, J., Jimenez-Navarro, M., Gomez, A., Smani, T. and Ordonez, A.: Cardioprotective Effect of Ranolazine in the Process of Ischemia-reperfusion in Adult Rat Cardiomyocytes.
, Rev Esp Cardiol (Engl Ed)
,
, 2015 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 251 u/ml |
| Medium | Tyrode solution |
Heart: Rat | |
| |
| Lee, J., Ha, J. and Leem, C.: A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat.
, Korean J Physiol Pharmacol
19,
373-82, 2015 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase: 0.08% Pronase: 0.004% |
| Medium | Tyrode's Solution |
Heart: Rat | |
| |
| Clark, A. and Naya, F.: MicroRNAs in the Myocyte Enhancer Factor 2 (MEF2)-regulated Gtl2-Dio3 Noncoding RNA Locus Promote Cardiomyocyte Proliferation by Targeting the Transcriptional Coactivator Cited2.
, J Biol Chem 290,
23162-72, 2015 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.025% Collagenase Type 2: 1.0% |
| Medium | HBSS |
Heart: Rat | |
| |
| Fang, R, Qiao, S., Liu, Y., Meng, Q., Chen, X., Song, B., Hou, X. and Tian, W.: Sustained Co-Delivery of BIO and IGF-1 by a Novel Hybrid Hydrogel System to Stimulate Endogenous Cardiac Repair in Myocardial Infarcted Rat Hearts.
, Int J Nanomedicine
10,
4691-703, 2015 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | DMEM |
Heart: Rat | |
| |
| Horne, T., VandeKopple, M., Sauls, K., Koenig, S., Anstine, L., Garg, V., Norris, R. and Lincoln, J.: Dynamic Heterogeneity of the Heart Valve Interstitial Cell Population in Mitral Valve Health and Disease, J Cardiovasc Dev Dis
2,
214, 2015 | |
| Cells: | Valve interstitial cells |
| Enzyme(s) | Collagenase Type 2: 600 u/ml |
| Medium | DMEM |
Heart: Rat | |
| |
| Miller, L., Greensmith, D., Sankaranarayanan, R., O'Neill, S. and Eisner D.: The Effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on Intracellular Ca2+ Handling in Rat Ventricular Myocytes.
, Cell Calcium
58,
208-14, 2015 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 95 u/ml Protease: 0.1 u/ml |
| Medium | HEPES |
Heart: Rat | |
| |
| Liu, L., Wang, Y., Cao, Z., Wang, M., Liu, X., Gao, T., Hu, Q., Yuan, W. and Lin, L.: Up-regulated TLR4 in Cardiomyocytes Exacerbates Heart Failure after Long-Term Myocardial Infarction.
, J Cell Mol Med
19,
2728, 2015 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Heart: Rat | |
| |
| Song, D., Lee, K., Ryu, J., Jeon, H. and Kim, D.: The Molecular Interaction of Heart LIM Protein (HLP) with RyR2 and Caveolin-3 is Essential for Ca(2+)-Induced Ca(2+) Release in the Heart.
, Biochem Biophys Res Commun
463,
975-81, 2015 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | HBSS |
Heart: Rat | |
| |
| Zhang, B., Montgomery, M., Davenport-Huyer, L., Korolj, A. and Radisic, M.: Platform Technology for Scalable Assembly of Instantaneously Functional Mosaic Tissues.
, Sci. Adv.
1,
e1500423, 2015 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.06% Collagenase Type 2: 220 u/ml |
| Medium | HBSS |
Heart: Rat | |
| |
| Rutering, J., Ilmer, M., Recio, A., Coleman, M., Vykoukal, J. and Alt, E.: Improved Method for Isolation of Neonatal Rat Cardiomyocytes with Increased Yield of C-Kit+ Cardiac Progenitor Cells.
, J Stem Cell Res Ther 5,
1-8, 2015 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.12% |
| Medium | DMEM/F-12 |
Heart: Rat | |
| |
| Sakurai, T., Lanahan, A., Woolls, M., Li, N., Tirziu, D. and Murakami, M.: Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction using Confocal Spinning Disk Microscopy.
, J Vis Exp
,
e51666, 2014 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Chouchani, E., Pell, V., Gaude, E., Aksentijevic, D., Sundier, S., Robb, E., Logan, A., Nadtochiy, S., Ord, E., Smith, A., Eyassu, F., Shirley, R., Hu, C., Dare, A., James, A. and Rogatti, S.: Ischaemic Accumulation of Succinate Controls Reperfusion Injury Through Mitochondrial ROS.
, Nature
515,
431-435, 2014 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | KH buffer |
Heart: Rat | |
| |
| Hennessey, J., Wei, E. and Pitt, G.: Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels.
, Circ Res 113,
381-8, 2013 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 150 u/ml |
| Medium | MEM |
Heart: Rat | |
| |
| Eckerle, L., Felix, S. and Herda, L.: Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes.
, J Vis Exp
73,
e4237, 2013 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 150 u/ml |
| Medium | see reference |
Heart: Rat | |
| |
| Zhang, B., Green, J., Murthy, S. and Radisic, M.: Label-Free Enrichment of Functional Cardiomyocytes using Microfluidic Deterministic Lateral Flow Displacement.
, PLoS ONE
7,
e37619, 2012 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.06% Collagenase Type 2: 220 u/ml |
| Medium | DMEM |
Heart: Rat | |
| |
| Karakikes, I., Hadri, L., Rapti, K., Ladage, D., Ishikawa, K., Tilemann, L., Yi, G., Morel, C., Gwathmey, J., Zsebo, K., Weber, T., Kawase, Y. and Hajjar, R.: Concomitant Intravenous Nitroglycerin with Intracoronary Delivery of AAV1.SERCA2a Enhances Gene Transfer in Porcine Hearts.
, Mol Ther
20,
565, 2012 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | Medium 199 |
Heart: Rat | |
| |
| Ye, K., Sullivan, K. and Black, L.: Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering.
, J Vis Exp
55,
3251, 2011 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 300 u/ml |
| Medium | PBS |
Heart: Rat | |
| |
| Mellor, K., Bell, J., Wendt, I., Davidoff, A., Ritchie, R. and Delbridge, L.: Fructose Modulates Cardiomyocyte Excitation-Contraction Coupling and Ca Handling In Vitro.
, PLoS ONE
6,
e25204, 2011 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.056% |
| Medium | Krebs |
Heart: Rat | |
| |
| Dittami, G., Rajguru, S., Lasher, R., Hitchcock, R. and Rabbitt, R.: Intracellular Calcium Transients Evoked by Pulsed Infrared Radiation in Neonatal Cardiomyocytes.
, J Physiol
589,
1295-306, 2011 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Guan, X., Delo, D., Atala, A. and Soker, S.: In Vitro Cardiomyogenic Potential of Human Amniotic Fluid Stem Cells.
, J Tissue Eng Regen Med
5,
220, 2011 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.07% Collagenase Type 2: 0.1% |
| Medium | DMEM/M199 |
Heart: Rat | |
| |
| Louch, W., Sheehan, K. and Wolska, B.: Methods in Cardiomyocyte Isolation, Culture, and Gene Transfer.
, J Mol Cell Cardiol 51,
288-98, 2011 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.025% |
| Medium | see reference |
Heart: Rat | |
| |
| Brinckmann, M., Kaschina, E., Altarche-Xifro, W., Curato, C., Timm, M., Grzesiak, A., Dong, J., Kappert, K., Kintscher, U., Unger, T. and Li, J.: Estrogen Receptor Alpha Supports Cardiomyocytes Indirectly Through Post-Infarct Cardiac c-kit+ Cells.
, J Mol Cell Cardiol 47,
66, 2009 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Smith, M., Huang, Y. and Deshmukh, M.: Skeletal Muscle Differentiation Evokes Endogenous XIAP to Restrict the Apoptotic Pathway.
, PLoS ONE
4,
e5097, 2009 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Xu, X. and Colecraft, H.: Primary Culture of Adult Rat Heart Myocytes.
, J Vis Exp
28,
1308, 2009 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% Protease XIV: 0.02% |
| Medium | Media 199 |
Heart: Rat | |
| |
| Farkas, A., Acsai, K., Nagy, N., Toth, A., Fulop, F., Seprenyi, G., Birinyi, P., Nanasi, P., Forster, T., Csanady, M., Papp, J., Varrro, A. and Farkas, A.: Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart.
, Br J Pharmacol 154,
93, 2008 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 1: 0.05% Hyaluronidase: see reference Protease: see reference |
| Medium | Krebs-Henseleit |
Heart: Rat | |
| |
| Kim, M., Oh, J., Sakata, S., Liang, I., Park, W., Hajjar, R. and Lebeche, D.: Role of Resistin in Cardiac Contractility and Hypertrophy.
, J Mol Cell Cardiol 45,
270, 2008 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Jang, J., Ku, S., Kim, J., Choi, K., Kim, Y., Kim, H., Oh, S., Lee, E., Cho, H., Song, Y., Lee, S., Lee, S., Suh, C., Kim, S., Moon, S. and Choi, Y.: Notch Inhibition Promotes Human Embryonic Stem Cell-Derived Cardiac Mesoderm Differentiation.
, Stem Cells 26,
2782, 2008 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM/F-12 |
Heart: Rat | |
| |
| Kubli, D., Quinsay, M., Huang, C., Lee, Y., Gustafsson, A.: Bnip3 Functions as a Mitochondrial Sensor of Oxidative Stress During Myocardial Ischemia and Reperfusion.
, Am J Physiol Heart Circ Physiol
295,
H2025, 2008 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | J-MEM |
Heart: Rat | |
| |
| LaFramboise, W., Scalise, D., Stoodley, P., Graner, S., Guthrie, R., Magovern, J and Becich, M.: Cardiac Fibroblasts Influence Cardiomyocyte Phenotype in Vitro.
, Am J Physiol Cell Physiol
292,
C1799-808, 2007 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% Pancreatin: 0.1% |
| Medium | DMEM |
Heart: Rat | |
| |
| Robinet, A., Millart, H., Oszust, F., Hornebeck, W. and Bellon, G.: Binding of Elastin Peptides to S-Gal Protects the Heart Against Ischemia/Reperfusion Injury by Triggering the RISK Pathway.
, FASEB J
21,
1968, 2007 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Jenkins, C., Milsted, A., Doane, K., Meszaros, G., Toot, J. and Ely, D.: A Cell Culture Model using Rat Coronary Artery Adventitial Fibroblasts to Measure Collagen Production.
, BMC Cardiovasc Disord
7,
13, 2007 | |
| Cells: | Ventricular fibroblasts |
| Enzyme(s) | Trypsin: 0.06% Collagenase: 100 u/ml |
| Medium | ADS Buffer:see reference |
Heart: Rat | |
| |
| Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA: An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction
, J Exp Med 203,
53-61, 2006 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% Hyaluronidase: 0.02% |
| Medium | DMEM |
Heart: Rat | |
| |
| Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M: Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation
, FASEB J
20,
744-6, 2006 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase: 0.13% Hyaluronidase: 0.06% |
| Medium | DMEM |
Heart: Rat | |
| |
| Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms
, J Physiol
575,
627-44, 2006 | |
| Cells: | Ventricular myocytes and mesenteric arterial SMC |
| Enzyme(s) | Collagenase Type 2: 0.06% Papain: 0.175% |
| Medium | Krebs-Ringer |
Heart: Rat | |
| |
| Butler, T., Au, C., Yang, B., Egan, J., Tan, Y., Hardeman, E., North, K., Verkman, A. and Winlaw, D.: Cardiac Aquaporin Expression in Humans, Rats, and Mice.
, Am J Physiol Heart Circ Physiol
291,
H705, 2006 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Pedram, A., Razandi, M., Aitkenhead, M., and Levin, E: Estrogen Inhibits Cardiomyocyte Hypertrophy in vitro. Antagonism of Calcineurin-related Hypertrophy through Induction of MCIP1
, J Biol Chem 280,
26339, 2005 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | DMEM/F-12 |
Heart: Rat | |
| |
| Head, B., Patel, H., Roth, D., Lai, N., Niesman, I., Farquhar, M. and Insel, P.: G-Protein-Coupled Receptor Signaling Components Localize in Both Sarcolemmal and Intracellular Caveolin-3-Associated Microdomains in Adult Cardiac Myocytes.
, J Biol Chem 280,
31036-44, 2005 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 250 u/ml |
| Medium | Cardioplegic solution |
Heart: Rat | |
| |
| Castillo, A., Ruzmetov, N., Harvey, K., Stillwell, W., Zaloga, G. and Siddiqui, R.: Docosahexaenoic Acid Inhibits Protein Kinase C Translocation/Activation and Cardiac Hypertrophy in Rat Cardiomyocytes.
, J Mol Genet Med 1,
18, 2005 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Entcheva, E., Bien, H, Yin L., Chung, C., Farrell, M., and Kostov, Y.: Functional Cardiac Cell Constructs on Cellulose-based Scaffolding
, Biomaterials 25(26),
5753, 2004 | |
| Cells: | Cardiomyocytes, fibroblasts |
| Enzyme(s) | Trypsin: 0.1% Collagenase: 0.1% |
| Medium | Medium 199 |
Heart: Rat | |
| |
| Muller-Bore, B., Cascio, W., Anderson, P., Snowwaert, J., Frye, J., Desai, N., Esch, G., Brackham, J., Bagnell, C., Coleman, W., Grisham, J., and Malouf, N.: Adult-derived Liver Stem Cells Acquire a Cardiomyocyte Structural and Functional Phenotype ex vivo
, Am J Pathol 165,
135, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Natarajan AR, Rong Q, Katchman AN, and Ebert SN.: Intrinsic cardiac catecholamines help maintain beating activity in neonatal rat cardiomyocyte cultures
, Pediatr Res 56,
411, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | DMEM |
Heart: Rat | |
| |
| Chen, Z., Ge, Y., and Kang J.: Down-regulation of the M6P/IGF-II Receptor Increases Cell Proliferation and Reduces Apoptosis in Neonatal Rat Cardiac Myocytes
, BMC Cell Biol 5,
15, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | F-10 |
Heart: Rat | |
| |
| Chen Hsiao-Huei, Mullett Steven J, Stewart Alexandre F R: Vgl-4, a novel member of the vestigial-like family of transcription cofactors, regulates alpha1-adrenergic activation of gene expression in cardiac myocytes
, J Biol Chem 279,
30800-6, 2004 | |
| Cells: | Cardiac myocytes |
| Enzyme(s) | Collagenase Type 2: 0.5% |
| Medium | HBSS |
Heart: Rat | |
| |
| Hunton DaciaL, Zou LuYun, Pang Yi, Marchase Richard B: Adult rat cardiomyocytes exhibit capacitative calcium entry
, Am J Physiol Heart Circ Physiol
286,
H1124-32, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 1: 0.03% Protease: 0.01% |
| Medium | HBSS |
Heart: Rat | |
| |
| Tamamori-Adachi, M., Hayashida, K., Nobori, K., Omizu, C., Yamada, K., Sakamoto, N., Kamura, T., Fukuda, K., Ogawa, S., Nakayama, K. and Kitajima, S.: Down-Regulation of p27 Promotes Cell Proliferation of Rat Neonatal Cardiomyocytes Induced by Nuclear Expression of Cyclin D1 and CDK4
, J Biol Chem 2279,
50429, 2004 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Heart: Rat | |
| |
| Aberle II NS, and Ren J.: Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy
, Biol Proced Online 5,
1, 2003 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase: 223 u/ml Hyaluronidase: 0.01% Trypsin: 0.002% |
| Medium | Medium 199 |
Heart: Rat | |
| |
| Li TS, Ito H, Kajiwara K, and Hamano K.: Long-term survival of xenografted neonatal cardiomyocytes by adenovirus-mediated CTLA4-Ig expression and CD40 blockade
, Circulation 108,
1760, 2003 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H: Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes
, Am J Physiol Heart Circ Physiol
284,
H330-6, 2003 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 178 u/ml Hyaluronidase: 0.01% |
| Medium | Krebs-Henseleit, CF |
Heart: Rat | |
| |
| Tamamori-Adachi Mimi, Ito Hiroshi, Sumrejkanchanakij Piyamas, Adachi Susumu, Hiroe Michiaki, Shimizu Masato, Kawauchi Junya, Sunamori Makoto, Marumo Fumiaki, Kitajima Shigetaka, Ikeda Masa-Aki: Critical role of cyclin D1 nuclear import in cardiomyocyte proliferation
, Circ Res 92,
e12-9, 2003 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | HEPES |
Heart: Rat | |
| |
| Swift Luther, McHowat Jane, Sarvazyan Narine: Inhibition of membrane-associated calcium-independent phospholipase A2 as a potential culprit of anthracycline cardiotoxicity
, Cancer Res 63,
5992-8, 2003 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05-0.1% |
| Medium | Joklik's MEM |
Heart: Rat | |
| |
| Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise: Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation
, Am J Physiol/Endo 284,
E1055-64, 2003 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% Deoxyribonuclease I: 0.02% |
| Medium | Krebs-Ringer |
Heart: Rat | |
| |
| DeAlmeida, A., Mustin, D., Forman, M., Brower, G., Janicki, J., and Carver, W.: Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression
, J Cell Physiol 191,
51, 2002 | |
| Cells: | Peritoneal mast |
| Enzyme(s) | Hyaluronidase: 100 u/ml |
| Medium | DMEM (see reference) |
Heart: Rat | |
| |
| Lam ML, Bartoli M, and Claycomb WC.: The 21-day postnatal rat ventricular cardiac muscle cell in culture as an experimental model to study adult cardiomyocyte gene expression
, Mol Cell Biochem 229,
51, 2002 | |
| Cells: | Ventricular cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.7-1% |
| Medium | DMEM |
Heart: Rat | |
| |
| Yang Y, Liao H, Ke Q, Cai J, Xiao YF, and Morgan JP: Enhancement of nitric oxide production by methylecgonidine in cultured neonatal rat cardiomyocytes
, Br J Pharmacol 135,
188, 2002 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Liu Qinghang, Hofmann Polly A: Antiadrenergic effects of adenosine A(1) receptor-mediated protein phosphatase 2a activation in the heart
, Am J Physiol Heart Circ Physiol
283,
H1314-21, 2002 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | Ringer solution |
Heart: Rat | |
| |
| Shimizu Tatsuya, Yamato Masayuki, Isoi Yuki, Akutsu Takumitsu, Setomaru Takeshi, Abe Kazuhiko, Kikuchi Akihiko, Umezu Mitsuo, Okano Teruo: Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces
, Circ Res 90,
e40, 2002 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 80u/ml |
| Medium | M199 |
Heart: Rat | |
| |
| Kitta, K., Day, R., Ikeda, T., and Suzuki, Y.: Hepatocyte Growth Factor Protects Cardiac Myocytes Against Oxidative Stress-Induced Apoptosis
, Free Radic Biol Med
31 (7),
902, 2001 | |
| Cells: | Cardiac myocytes |
| Enzyme(s) | Protease: 0.55 u/ml |
| Medium | Tyrode's solution |
Heart: Rat | |
| |
| Schwartzbauer, G., and Robbins, J.: The Tumor Suppressor Gene PTEN Can Regulate Cardiac Hypertrophy and Survival
, J Biol Chem 276,
35786, 2001 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: |
| Medium | DMEM/Ham's F-12 |
Heart: Rat | |
| |
| Rahman A, Alam M, Rao S, Cai L, Clark LT, Shafiq S, and Siddiqui MA: Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro
, Biol Res 34(3-4),
195, 2001 | |
| Cells: | Cardiac myocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Ren J, and Wold LE.: Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging
, Biol Proced Online 3,
43, 2001 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase: 223 u/ml Hyaluronidase: 0.01% Trypsin: 0.002% |
| Medium | Tyrode solution |
Heart: Rat | |
| |
| Dai L, Brookes PS, Darley-Usmar VM, and Anderson PG.: Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of NO
, Am J Physiol/Heart 281,
H2261-H2269, 2001 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 140 u/ml |
| Medium | Krebs-Henseleit |
Heart: Rat | |
| |
| Guo K, Searfoss G, Krolikowski D, Pagnoni M, Franks C, Clark K, Yu KT, Jaye M, Ivashchenko Y: Hypoxia induces the expression of the pro-apoptotic gene BNIP3
, Cell Death Differ
8,
367-76, 2001 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | DMEM/M199 |
Heart: Rat | |
| |
| Pyle, W, Lester, J, and Hofmann, P.: Effects of Kappa-opioid receptor activation of myocardium
, Am J Physiol Heart Circ Physiol
281,
H669, 2001 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | Ringer solution |
Heart: Rat | |
| |
| Arutunyan A, Webster D, Swift L, Sarvazyan N: Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias
, Am J Physiol Heart Circ Physiol
280,
H1905-15, 2001 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.01% Collagenase Type 2: 0.08% |
| Medium | HBSS |
Heart: Rat | |
| |
| Kim, D., and Pleumsamran, A.: Cytoplasmic Unsaturated Free Fatty Acids Inhibit ATP-Dependent Gating of the G Protein-gated K+ Channel
, J Gen Physiol 115,
287, 2000 | |
| Cells: | Atrial |
| Enzyme(s) | Trypsin: 0.06% |
| Medium | DMEM |
Heart: Rat | |
| |
| Bueno OF, De Windt LJ, Tymitz KM, Witt SA, Kimball TR, Klevitsky R, Hewett TE, Jones SP, Lefer DJ, Peng CF, Kitsis RN, and Molkentin JD.: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice
, EMBO J
19(23),
6341, 2000 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 1: 84 u/ml Pancreatin: 0.05% |
| Medium | M199 medium |
Heart: Rat | |
| |
| Maki T, Horio T, Yoshihara F, Suga S, Takeo S, Matsuo H, and Kangawa K: Effect of neutral endopeptidase inhibitor on endogenous atrial natriuretic peptide as a paracrine factor in cultured cardiac fibroblasts
, Br J Pharmacol 131(6),
1204, 2000 | |
| Cells: | Ventricular myocytes |
| Enzyme(s) | Collagenase Type 2: 0.08% |
| Medium | DMEM |
Heart: Rat | |
| |
| Puri S, Bansal DD, Uskokovic MR, and MacGregor RR.: Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3)
, Am J Physiol/Endo 278(2),
E293, 2000 | |
| Cells: | Heart microvascular cells |
| Enzyme(s) | CLSPA: 250u/ml Papain: 5 u/ml Elastase: 0.8 u/ml |
| Medium | L-15 |
Heart: Rat | |
| |
| Sun L, Chang J, Kirchhoff SR, and Knowlton AA.: Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment
, Am J Physiol Heart Circ Physiol
278(4),
H1091, 2000 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.06% |
| Medium | M199 |
Heart: Rat | |
| |
| Cowan DB, Poutias DN, Del Nido PJ, and McGowan FX Jr: CD14-independent activation of cardiomyocyte signal transduction by bacterial endotoxin
, Am J Physiol Heart Circ Physiol
279,
H619, 2000 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | DMEM-F12 |
Heart: Rat | |
| |
| Webster DR, and Patrick DL.: Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset
, Am J Physiol Heart Circ Physiol
278,
H1653, 2000 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Heart: Rat | |
| |
| Kim, B., Nikolovski, J., Bonadio, J., Smiley, E., and Mooney, D.: Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold
, Exp Cell Res
251,
318, 1999 | |
| Cells: | Aorta, smooth muscle |
| Enzyme(s) | Trypsin: 0.025% |
| Medium | Medium 199 with 20% FBS medium |
Heart: Rat | |
| |
| Nemec, J., Wickman, K., and Clapham, D.: GBy Binding Increases the Open Time of IKACh: Kinetic Evidence for Multiple GBy Binding Sites
, Biophys J
76,
246, 1999 | |
| Cells: | Atrial |
| Enzyme(s) | Trypsin: 0.03% |
| Medium | HBSS or EBSS |
Heart: Rat | |
| |
| Adderley SR, and Fitzgerald DJ.: Oxidative damage of cardiomyocytes is limited by extracellular regulated kinases 1/2-mediated induction of cyclooxygenase-2
, J Biol Chem 274,
5038, 1999 | |
| Cells: | Neonatal rat cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 80u/ml Pancreatin: 0.06% |
| Medium | DMEM/F12 |
Heart: Rat | |
| |
| Reinecke H, Zhang M, Bartosek T, and Murry CE.: Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts
, Circulation 100(2),
193, 1999 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Pancreatin: 0.06% Collagenase Type 2: 95 u/ml |
| Medium | DMEM |
Heart: Rat | |
| |
| Richards SM, Jaconi ME, Vassort G, and Puceat M.: A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes
, J Cell Sci
112,
1519, 1999 | |
| Cells: | Cardioomyocytes |
| Enzyme(s) | Collagenase Type 4: 0.12%-adult Collagenase Type 2: 0.05%-neonatal |
| Medium | DMEM/medium 199 |
Heart: Rat | |
| |
| Sambandam N, Abrahani MA, St Pierre E, Al-Atar O, Cam MC, and Rodrigues B.: Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin
, Arterioscler Thromb Vasc Biol
19(6),
1526, 1999 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 228 u/ml |
| Medium | Joklik MEM |
Heart: Rat | |
| |
| Matsui, T., Li, Ling, Del Monte, F., Fukui, Y., Franke, T., Hajjar, R., Rosenzweig, A.: Adenoviral gene transfer of activated phosphatidylinositol 3'-kinase and Akt inhibits apoptosis of hypoxic cardiomyocytes in vitro
, Circulation 100,
2373-9, 1999 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | RPMI |
Heart: Rat | |
| |
| Zilberman, A., Dave, V., Miano, J., Olson, E., and Periasamy, M.: Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression
, Circ Res 82,
566, 1998 | |
| Cells: | Thoracic aorta, smooth muscle |
| Enzyme(s) | Elastase: 0.02% |
| Medium | (see reference) |
Heart: Rat | |
| |
| Wagner DR, Combes A, McTiernan C, Sanders VJ, Lemster B, Feldman AM: Adenosine inhibits lipopolysaccharide-induced cardiac expression of tumor necrosis factor-alpha
, Circ Res 82,
47-56, 1998 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | DMEM/F-12 |
Heart: Rat | |
| |
| Yu L, Netticadan T, Xu YJ, Panagia V, Dhalla NS: Mechanisms of lysophosphatidylcholine-induced increase in intracellular calcium in rat cardiomyocytes
, J Pharmacol Exp Ther 286,
1-8, 1998 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.04% |
| Medium | see reference |
Heart: Rat | |
| |
| Westfall M V, Rust E M, Metzger J M: Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function
, Proc Natl Acad Sci U S A 94,
5444-9, 1997 | |
| Cells: | Ventricular cardiac myocytes |
| Enzyme(s) | Collagenase Type 2: 0.5% Hyaluronidase: 0.2% |
| Medium | Krebs-Henseleit buffer |
Heart: Rat | |
| |
| Kinugawa, K., Kohmoto, O., Yao, A., Serizawa, T., and Takahashki, T.: Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes
, Am J Physiol 272,
H35-47, 1997 | |
| Cells: | Cardiomyoctes |
| Enzyme(s) | Collagenase Type 2: 80 u/ml |
| Medium | HBSS |
Heart: Rat | |
| |
| Vanwinkle, W., Snuggs, M., and Buja, L.: Cardiogel: A Biosynthetic Extracellular Matrix for Cardiomyocyte Culture
, In Vitro Cell Dev Biol Anim 32,
478, 1996 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Heart: Rat | |
| |
| Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH, and Sheu SS: Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes
, Circ Res 79(1),
86, 1996 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | Joklik's |
Heart: Rat | |
| |
| Li RK, Mickle DA, Weisel RD, Zhang J, Mohabeer MK: In vivo survival and function of transplanted rat cardiomyocytes
, Circ Res 78,
283-8, 1996 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase: 0.1% Trypsin: 0.2% |
| Medium | PBS |
Heart: Rat | |
| |
| MacGregor, R., Klein, R., and Bansal, D.: Secretion of Plasminogen Activator Activity from Neonatal Rat Heart Cells is Regulated by Hormones and Growth Factors
, Ann N Y Acad Sci 752,
331, 1995 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | HBSS |
Heart: Rat | |
| |
| Laughlin, M., Schaeffer, M., and Sturek, M.: Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats
, Am J Physiol 73,
1141, 1992 | |
| Cells: | Myocytes Ventricles |
| Enzyme(s) | Collagenase: 0.07% |
| Medium | Joklik's MEM |
Heart: Rat | |
| |
| Welder, A.A., Grant, R., Bradlaw, J., and Acosta, D.: A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents
, In Vitro Cell Dev Biol 27,
921, 1991 | |
| Cells: | Myocardial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | Joklik's MEM, Kreb's-Henseleit buffer, CF |
Heart: Rat | |
| |
| Fischer, Y., Rose, H., and Kammermeier. H.: Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method
, Life Sci 49,
1679, 1991 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase: 0.11% |
| Medium | (see reference) |
Heart: Rat | |
| |
| Marino, T.A., Walter, R.,Cobb, E., Palasiuk, M., Parsons, T., and Mercer, W.E.: Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation
, In Vitro Cell Dev Biol 26,
229, 1990 | |
| Cells: | Cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.12% |
| Medium | Krebs-Henseleit Buffer, CF |
Heart: Rat | |
| |
| Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons
, In Vitro Cell Dev Biol 26,
335, 1990 | |
| Cells: | Heart Adrenal chromaffin Paraneurons |
| Enzyme(s) | Trypsin: 0.06% |
| Medium | 25mM HEPES buffered Locke's solution, CMF |
Heart: Rat | |
| |
| Nag, A.C., Lee, M., and Kosiur, J.R.: Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms
, In Vitro Cell Dev Biol 26,
464, 1990 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | Joklik's MEM |
Heart: Rat | |
| |
| Engelmann, G., McTiernan, C., Gerrity, R., and Samarel, A.: Serum-free Primary Cultures of Neonatal Rat Cardiomyocytes: Cellular and Molecular Applications, Technique 2 (6),
279, 1990 | |
| Cells: | Ventricular cardiomyocytes |
| Enzyme(s) | Collagenase Type 2: 0.05 - 0.08% |
| Medium | See reference |
Heart: Rat | |
| |
| Berg, I., Guse, A.H., and Gercken, G.: Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats
, Biochim Biophys Acta 1010,
100, 1989 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 1: 100 u/ml and 150 u/ml |
| Medium | Krebs Ringer bicarbonate buffer |
Heart: Rat | |
| |
| Bugaisky, L.B. and Zak, R.: Differentiation of Adult Rat Cardiac Myocytes in Cell Culture
, Circ Res 64,
493, 1989 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Joklik's medium |
Heart: Rat | |
| |
| De Young, M., Giannattasio, B., and Scarpa, A.: Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart
,Methods Enzymology Vol. 173,, ,
662, 1989 | |
| Cells: | Myocytes, ventricular |
| Enzyme(s) | Collagenase Type 1: 90 and 100 u/ml |
| Medium | Joklik's solution with and without calcium |
Heart: Rat | |
| |
| Toraason, M., Luken, M., Breitenstein, M., Krueger, J., and Biagini, R.: Comparative Toxicity of Allylamine and Acrolein in Cultured Myocytes and Fibroblasts from Neonatal Rat Heart
, Toxicology 56,
107, 1989 | |
| Cells: | Cardiomyocytes Fibroblasts |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS |
Heart: Rat | |
| |
| Cornwell, T., and Lincoln, T.: Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells
, J Biol Chem 264 (2),
1146, 1989 | |
| Cells: | Aortic smooth muscle |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Heart: Rat | |
| |
| McMahon, K.K.: A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, Eds), Elsevier Science Publishing Co. Inc.,
318, 1988 | |
| Cells: | Myocytes, atria |
| Enzyme(s) | Collagenase Type 1: 100 u/ml |
| Medium | Kreb's Ringer bicarbonate - HEPES buffer |
Heart: Rat | |
| |
| Buxton, I.L.O. and Doggwiler, K.O.: Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), ,
298, 1988 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | Kreb's Ringer w/ calcium |
Heart: Rat | |
| |
| Kim, D. and Smith, T.W.: Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), Elsevier Science Publishing Co. Inc.,
370, 1988 | |
| Cells: | Myocytes, heart |
| Enzyme(s) | Hyaluronidase: 0.03% |
| Medium | Bicarbonate-buffered medium |
Heart: Rat | |
| |
| Grynberg, A., Degois, M., Guenot, L., and Athias, P.: Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates
, Biol Cell 57,
89, 1986 | |
| Cells: | Myoblast, cardiac |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Standardized Medium (see reference) |
Heart: Rat | |
| |
| Freerksen, D., Schroedl, N., and Hartzell, C.: Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium
, J Cell Physiol 120,
126, 1984 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 2.4 u/ml |
| Medium | DMEM |
Heart: Rat | |
| |
| Piper, H.M., Probst, I., Schwartz, P., Hutter, F.J., and Spieckermann, P.G.: Culturing of Calcium Stable Adult Cardiac Myocytes
, J Mol Cell Cardiol 14,
397, 1982 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 1: 0.06% |
| Medium | Krebs Ringer bicarbonate buffer |
Heart: Rat | |
| |
| Nag, A. and Cheng, M.: Adult Mammalian Cardiac Muscle Cells in Culture
, Tissue Cell
13,
515, 1981 | |
| Cells: | Muscle |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Krebs Ringer phosphate buffer, CMF |
Heart: Rat | |
| |
| Claycomb, W.C., and Palazzo, M.C.: Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study
, Dev Biol 80,
466, 1980 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | Joklik's MEM |
Heart: Rat | |
| |
| Frangakis, C., Bahl, J., McDaniel, H., and Bressler, R.: Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation
, Life Sci 27,
815, 1980 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.10% |
| Medium | Joklik MEM, CF |
Heart: Rat | |
| |
| Powell, T., Terrar, D.A., and Twist, V.W.: Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium
, J Physiol
302,
131, 1980 | |
| Cells: | Myocytes Ventricular myocardium |
| Enzyme(s) | Collagenase Type 1: 0.20% |
| Medium | Kreb's Ringer bicarbonate buffer |
Heart: Rat | |
| |
| Bishop, S., Oparil, S., Reynolds, R., and Drummond, J.: Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats
, Hypertension 1 (4),
378, 1979 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | Saline |
Heart: Rat | |
| |
| Farmer, B., Harris, R., Jolly, W., Hathaway, D., Katzberg, A., Watanabe, A., Whitlow, A., and Besch, H.: Isolation and Characterization of Adult Rat Heart Cells
, Arch Biochem Biophys
179,
545, 1977 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 82 u/ml |
| Medium | Kreb's Henseleit buffer |
Heart: Rat | |
| |
| Nag, A., Fischman, D., Aumont, M., and Zak, R.: Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability
, Tissue Cell
9 (3),
419, 1977 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Kreb's Ringers phosphate |
Heart: Rat | |
| |
| Grosso, D., Frangakis, C., Carlson, E., and Bressler, R.: Isolation and Characterization of Myocytes From the Adult Rat Heart
, Prep Biochem 7 (5),
383, 1977 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase: 0.1% Hyaluronidase: 0.1% |
| Medium | MEM CF |
Heart: Rat | |
| |
| Moustafa, E., Skomedal, T., Osnes, J., and Oye, I.: Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+
, Biochim Biophys Acta 421,
411, 1976 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Perfusing solution |
Heart: Rat | |
| |
| Glick, M., Burns, A., and Reddy, W.: Dispersion and Isolation of Beating Cells from Adult Rat Heart
, Anal Biochem
61,
32, 1974 | |
| Cells: | Heart ventricles, beating |
| Enzyme(s) | Collagenase: 0.05-0.2% |
| Medium | Phosphate buffer |
Heart: Rat | |
| |
| Bierman, E.L., Stein, O., and Stein, Y.: Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture
, Circ Res 35,
136, 1974 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Versene buffer |
Heart: Rat | |
| |
| Speicher, D., and McCarl, R.: Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture
, In Vitro 10,
30, 1974 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 0.01% |
| Medium | Saline A |
Heart: Rat | |
| |
| Pretlow II, T., Glick, M., and Reddy, W.: Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells
, Am J Pathol 67 (2),
215, 1972 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.05% |
| Medium | (see reference) |
Heart: Rat | |
| |
| Berry, M., Friend, D., and Scheuer, J.: Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart
, Circ Res 26,
679, 1970 | |
| Cells: | Myocytes |
| Enzyme(s) | Hyaluronidase: 0.20% |
| Medium | Hank's solution, CF |
Heart: Rat | |
| |
| Vahouny, G.V., Wei, R., Starkweather, R., and Davis, C.: Preparation of Beating Heart Cells From Adult Rats
, Science 167,
1616, 1970 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Saline A |
Heart: Rat | |
| |
| Mark, G., and Strasser, F.: Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells
, Exp Cell Res
44,
217, 1966 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 0.125% |
| Medium | Gey's BSS |
Heart: Shellfish | |
| |
| Le Duff, R., Lipart, C., and Renault, T.: Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells, J Tiss Cul Meth 16,
67, 1994 | |
| Cells: | Haemocytes |
| Enzyme(s) | Trypsin: |
| Medium | L15 medium |
Heart: Shellfish | |
| |
| Wen, C., Kou, G., and Chen, S.: Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding), J Tiss Cul Meth 15,
123, 1993 | |
| Cells: | Myocytes Fibroblasts |
| Enzyme(s) | Collagenase Type 1: 2% |
| Medium | L15 medium |
Intestine: Canine | |
| |
| Damholt AB, Buchan AM, Kofod H: Glucagon-like-peptide-1 secretion from canine L-cells is increased by glucose-dependent-insulinotropic peptide but unaffected by glucose
, Endocrinology 139,
2085-91, 1998 | |
| Cells: | Intestinal L-cells |
| Enzyme(s) | Collagenase Type 1: 75 u/ml |
| Medium | HBSS |
Intestine: Human | |
| |
| Doyle, C., Vine, E, Bertram, K., Baharlou, H., Rhodes, J., Dervish, S., Gosselink, M., Di Re, A., Collins, G., Reza, F., Toh, J., Pathma-Nathan, N., Ahlenstiel, G., Ctercteko, G., Cunningham, A., Harm, A and Byrne, S.: Optimal Isolation Protocols for Examining and Interrogating Mononuclear Phagocytes From Human Intestinal Tissue.
,Front Immunol Vol. 12,, ,
727952, 2021 | |
| Cells: | |
| Enzyme(s) | Collagenase Type 4: 0.3% Deoxyribonuclease I: 5 ul/ml |
| Medium | |
Intestine: Human | |
| |
| Trapecar, M., Khan, S., Roan, N., Chen, T., Telwatte, S., Deswal, M., Pao, M., Somsouk, M., Deeks, S., Hunt, P., Yukl, S. and Sanjabi, S.: An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies.
, AIDS Res Hum Retroviruses 33,
S31-S39, 2017 | |
| Cells: | Lymphocytes |
| Enzyme(s) | CLSPA: 0.05% Deoxyribonuclease I: 0.00075% |
| Medium | RPMI-1640 |
Intestine: Human | |
| |
| Kessler, S., Rho, H., West, G., Fiocchi, C., Drazba, J. and de la Motte, C.: Hyaluronan (HA) Deposition Precedes and Promotes Leukocyte Recruitment in Intestinal Inflammation.
, Clin Transl Sci 1,
57-61, 2008 | |
| Cells: | Microvessel endothelial |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | HBSS |
Intestine: Human | |
| |
| Ebert, Ellen.: CD2 Activation of Human Lamina Propria Lymphocytes Reduces CD3 Responsiveness.
, Immunology 117,
71-7, 2006 | |
| Cells: | Lamina propria lymphocytes |
| Enzyme(s) | Collagenase: 20 u/ml |
| Medium | Medium |
Intestine: Human | |
| |
| Kanai Takanori, Totsuka Teruji, Uraushihara Koji, Makita Shin, Nakamura Tetsuya, Koganei Kazutaka, Fukushima Tsuneo, Akiba Hisaya, Yagita Hideo, Okumura Ko, Machida Utako, Iwai Hideyuki, Azuma Miyuki, Chen Lieping, Watanabe Mamoru: Blockade of B7-H1 suppresses the development of chronic intestinal inflammation
, J Immunol 171,
4156-63, 2003 | |
| Cells: | Lamina propria mononuclear cells |
| Enzyme(s) | Collagenase: 0.02% |
| Medium | HBSS |
Intestine: Human | |
| |
| Fahlgren, A., Hammarstrom, S., Danielsson, A. and Hammarstrom, M.: Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis.
, Clin Exp Immunol 131,
90, 2003 | |
| Cells: | Intestinal epithelial |
| Enzyme(s) | Collagenase Type 4: 72.5 u/ml |
| Medium | HBSS |
Intestine: Human | |
| |
| Stallmach A, Schaffer F, Hoffmann S, Weber S, Muller-Molaian I, Schneider T, Kohne G, Ecker KW, Feifel G, Zeitz M: Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis
, Gut 43,
499-505, 1998 | |
| Cells: | Mucosal mononuclear cells |
| Enzyme(s) | Collagenase Type 3: 0.01% Deoxyribonuclease I: 0.01% Soybean Trypsin Inhibitor: 0.01% |
| Medium | RPMI |
Intestine: Mouse | |
| |
| Emgard, J., Kammoun, H., Garcia-Cassani, B., Chesne, J., Parigi, S., Jacob, J., Cheng, H, Evren, E., Das, S., Czarnewski, P., Sleiers, N. Melo-Gonzalez, F., Kvedaraite, E. Svensson, M. and Scandella, E.: Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation.
, Immunity
48,
120-132.e8, 2018 | |
| Cells: | Lamina propria lymphocytes |
| Enzyme(s) | Collagenase Type 4: 0.02% Deoxyribonuclease I: 0.01% |
| Medium | HBSS |
Intestine: Mouse | |
| |
| Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J and Ming L: Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase.
, Braz J Med Biol Res
50,
e5831, 2017 | |
| Cells: | Crypt |
| Enzyme(s) | Collagenase Type 1: 200 u/ml Hyaluronidase: 100u/ml |
| Medium | DMEM |
Intestine: Mouse | |
| |
| O'Rourke, K., Ackerman, S., Dow, L. and Lowe, S.: Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells.
, Bio Protoc
6,
, 2016 | |
| Cells: | Intestinal organoids |
| Enzyme(s) | Collagenase Type 4: 500 u/ml Deoxyribonuclease I: 15 u/ml |
| Medium | Basal Medium |
Intestine: Mouse | |
| |
| Seo, S., Kuffa, P., Kitamoto, S., Nagao-Kitamoto, H., Rousseau, J., Kim, Y., Nunez, G. and Kamada, N.: Intestinal Macrophages Arising from CCR2(+) Monocytes Control Pathogen Infection by Activating Innate Lymphoid Cells.
, Nat Commun
6,
8010, 2015 | |
| Cells: | Lamina propria mononuclear cells |
| Enzyme(s) | Collagenase Type 3: 400 u/ml Deoxyribonuclease I: 0.001% |
| Medium | HBSS |
Intestine: Mouse | |
| |
| Beura, L., Anderson, K., Schenkel, J., Locquiao, J., Fraser, K., Vezys, V., Pepper, M. and Masopust, D.: Lymphocytic Choriomeningitis Virus Persistence Promotes Effector-Like Memory Differentiation and Enhances Mucosal T cell Distribution.
, J Leukoc Biol 97,
217, 2015 | |
| Cells: | Lymphocytes |
| Enzyme(s) | Collagenase Type 1: 100 u/ml |
| Medium | RPMI-1640 |
Intestine: Mouse | |
| |
| Hotta, R., Stamp, L., Foong, J., McConnell, S., Bergner, A., Anderson, R., Enomoto, H., Newgreen, D., Obermayr, F., Furness, J. and Young, H.: Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon.
, J Clin Invest 123,
1182, 2013 | |
| Cells: | Enteric neural crest progenitors |
| Enzyme(s) | Neutral Protease: 0.5% Collagenase animal free: 0.05% |
| Medium | DMEM/F12 |
Intestine: Mouse | |
| |
| Xue, X. and Shah, Y.: In Vitro Organoid Culture of Primary Mouse Colon Tumors.
, J Vis Exp
,
e50210, 2013 | |
| Cells: | Colon tumor organoids |
| Enzyme(s) | Collagenase Type 4: 200 u/ml Neutral Protease: 0.01% |
| Medium | DMEM |
Intestine: Mouse | |
| Zhang, Y. and Hu, W.: Mouse Enteric Neuronal Cell Culture.
, Methods Mol Biol 1078,
55-63, 2013 | |
| Cells: | Enteric neurons |
| Enzyme(s) | Collagenase Type 4: 0.1% Trypsin: 0.05% Deoxyribonuclease I: 400 u/ml |
| Medium | DMEM/F12 |
Intestine: Mouse | |
| |
| Barthel, E., Speer, A., Levin, D., Sala, F., Hou, X., Torashima, Y., Wigfall, C. and Grikscheit, T.: Tissue Engineering of the Intestine in a Murine Model.
, J Vis Exp
70,
e4279, 2012 | |
| Cells: | Intestinal organoids |
| Enzyme(s) | Collagenase Type 1: 800 u/ml Neutral Protease: 0.013% |
| Medium | DMEM |
Intestine: Mouse | |
| |
| Geem, D., Medina-Contreras, O., Kim, W., Huang, C. and Denning T.: Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine.
, J Vis Exp
63,
e4040, 2012 | |
| Cells: | Dendritic, macrophages |
| Enzyme(s) | Collagenase: 0.15% Deoxyribonuclease I: 0.004% |
| Medium | HBSS |
Intestine: Mouse | |
| |
| Ruiz, V., Sachdev, M., Zhang, S., Wen, S. and Moss, S.: Isolating, Immunophenotyping and Ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes During Murine Helicobacter Pylori Infection.
, J Immunol Methods 384,
157-63, 2012 | |
| Cells: | Lyphocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% Neutral Protease: 0.1% Deoxyribonuclease I: 200 ug/ml |
| Medium | RPMI 1640 |
Intestine: Mouse | |
| |
| Becker, L., Kulkarni, S., Tiwari, G., Micci, M. and Pasricha, P.: Divergent Fate and Origin of Neurosphere-Like Bodies from Different Layers of the Gut.
, Am J Physiol Gastrointest Liver Physiol
302,
G958-65, 2012 | |
| Cells: | Enteric neural stem cells |
| Enzyme(s) | Collagenase: 0.11% Neutral Protease: 0.1% Deoxyribonuclease I: 50 u/ml |
| Medium | M199 |
Intestine: Mouse | |
| |
| Joseph, N., He, S., Quintana, E., Kim, Y., Nunez, G. and Morrison, S.: Enteric Glia are Multipotent in Culture but Primarily Form Glia in the Adult Rodent Gut.
, J Clin Invest 121,
3398, 2011 | |
| Cells: | Myenteric plexus |
| Enzyme(s) | Collagenase Type 4: 0.025-0.1% Papain: 10 u/ml Deoxyribonuclease I: 100 u/ml |
| Medium | HBSS |
Intestine: Mouse | |
| |
| Ito, Y., Kanai, T., Totsuka, T., Okamoto, R., Tsuchiya, K., Nemoto, Y., Yoshioka, A., Tomita, T., Nagaishi, T., Sakamoto, N., Sakanishi, T., Okumura, K., Yagita, H. and Watanabe, M.: Blockade of NKG2D Signaling Prevents the Development of Murine CD4+ T Cell-Mediated Colitis.
, Am J Physiol Gastrointest Liver Physiol
294,
G199, 2008 | |
| Cells: | Lamina propria lymphocytes |
| Enzyme(s) | Collagenase Type 1: 0.3% Deoxyribonuclease I: 0.01% |
| Medium | RPMI 1640 |
Intestine: Mouse | |
| |
| Forbes, E., Groschwitz, K., Abonia, J.P., Brandt, E., Cohen, E., Blanchard, C., Ahrens, R., Seidu, L., McKenzie, A., Strait, R., Finkelman, F., Foster, P., Matthaei, K., Rothenberg, M. and Hogan, S.: IL-9- and Mast Cell-Mediated Intestinal Permeability Predisposes to Oral Antigen Hypersensitivity.
, J Exp Med 205,
897, 2008 | |
| Cells: | Intestinal mononuclear |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | RPMI 1640 |
Intestine: Mouse | |
| |
| Kidd, M., Modlin, I., Eick, G. and Champaneria, M.: Isolation, Functional Characterization, and Transcriptome of Mastomys Ileal Enterochromaffin Cells.
, Am J Physiol Gastrointest Liver Physiol
291,
G778-91, 2006 | |
| Cells: | Enterochromaffin cells |
| Enzyme(s) | Collagenase: 0.025% Pronase E: 0.07% |
| Medium | HBSS |
Intestine: Mouse | |
| |
| Goto Kazunori, Matsuoka Satoshi, Noma Akinori: Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine
, J Physiol
559,
411-22, 2004 | |
| Cells: | Interstitial cells |
| Enzyme(s) | Collagenase: 0.04% Trypsin: 0.02% |
| Medium | see reference |
Intestine: Mouse | |
| |
| Ordag Tamas, Redelman Doug, Miller Lisa J, Horvath Viktor J, Zhong Qiao, Almeida-Porada Graca, Zanjani Esmail D, Horowitz Burton, Sanders Kenton M: Purification of interstitial cells of Cajal by fluorescence-activated cell sorting
, Am J Physiol Cell Physiol
286,
C448-56, 2004 | |
| Cells: | Interstitial cells of Cajal |
| Enzyme(s) | Collagenase Type 2: 0.13% |
| Medium | HBSS |
Intestine: Mouse | |
| |
| Lee Young Mee, Kim Byung Joo, Kim Hyun Jin, Yang Dong Ki, Zhu Mei Hong, Lee Kyu Pil, So Insuk, Kim Ki Whan: TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach
, Am J Physiol/Gastro 284,
G604-16, 2003 | |
| Cells: | Interstitial cells of Cajal |
| Enzyme(s) | Collagenase Type 2: 0.13% |
| Medium | Hanks |
Intestine: Mouse | |
| |
| Wu Y, Wang X, Csencsits KL, Haddad A, Walters N, Pascual DW: M cell-targeted DNA vaccination
, Proc Natl Acad Sci U S A 98,
9318-23, 2001 | |
| Cells: | Lamina propria lymphocytes |
| Enzyme(s) | Collagenase Type 4: 300 u/ml |
| Medium | PBS |
Intestine: Mouse | |
| |
| Sakagami Y, Inaguma Y, Sakakura T, Nishizuka Y: Intestine-like remodeling of adult mouse glandular stomach by implanting of fetal intestinal mesenchyme
, Cancer Res 44,
5845-9, 1984 | |
| Cells: | Intestinal mesenchymal |
| Enzyme(s) | Collagenase Type 2: 0.03% |
| Medium | HBSS |
Intestine: Rat | |
| |
| Kruger, G., Mosher, J., Bixby, S., Joseph, N., Iwashita, T. and Morrison, S.: Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness.
, Neuron 35,
657, 2002 | |
| Cells: | Gut neural crest stem |
| Enzyme(s) | Collagenase Type 4: 0.1% Trypsin: 0.025% |
| Medium | HBSS |
Intestine: Rat | |
| |
| Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity.
, Neuron 35,
643, 2002 | |
| Cells: | Sciatic nerve and gut neural crest stem |
| Enzyme(s) | Collagenase Type 4: 0.025% Trypsin: 0.005% Deoxyribonuclease I: 0.05% |
| Medium | HBSS |
Intestine: Rat | |
| |
| Quaroni A, Wands J, Trelstad RL, Isselbacher KJ: Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria
, J Cell Biol
80,
248-65, 1979 | |
| Cells: | Intestinal epithelial |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Kidney: Avian | |
| |
| Goldstein D., Reddy V., and Plaga K.: Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones
, Am J Physiol 276,
R847, 1999 | |
| Cells: | Tubule segments |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | HBSS |
Kidney: Avian | |
| |
| Reich, C. and Bonar, R.: Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture, Meth Cell Sci 7,
97, 1982 | |
| Cells: | Tubule and glomeruli |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | RPMI 1640 |
Kidney: Bovine | |
| |
| Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture
, Am J Physiol 255,
F1160, 1988 | |
| Cells: | Papillary duct |
| Enzyme(s) | Hyaluronidase: 0.2% |
| Medium | Keri's buffer HEPES buffered saline |
Kidney: Canine | |
| |
| Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.
, Lipids Health Dis
10,
171, 2011 | |
| Cells: | Renal adipose derived cells |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Kidney: Canine | |
| |
| States, B., Reynolds, R., Lee, J., and Segal, S.: Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments
, In Vitro Cell Dev Biol 26,
105, 1990 | |
| Cells: | Proximal tubules |
| Enzyme(s) | Collagenase Type 1: 0.15% |
| Medium | Krebs Ringer bicarbonate buffer |
Kidney: Canine | |
| |
| Yau, C., Rao, L., and Silverman, M.: Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells
, Can J Physiol Pharmacol 63,
417, 1985 | |
| Cells: | Proximal tubular |
| Enzyme(s) | Deoxyribonuclease I: 0.0125% |
| Medium | (see reference) |
Kidney: Fish | |
| |
| Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport
, Am J Physiol 251,
424, 1986 | |
| Cells: | Renal tubule |
| Enzyme(s) | Trypsin: 0.2% Trypsin: 0.2% |
| Medium | CMF solution |
Kidney: Guinea-Pig | |
| |
| Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase
, In Vitro 8,
101, 1972 | |
| Cells: | Single cells |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | CF salt solution |
Kidney: Hamster | |
| |
| Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture
, Am J Physiol 255,
F1160, 1988 | |
| Cells: | Papillary duct |
| Enzyme(s) | Hyaluronidase: 0.2% |
| Medium | Keri's buffer HEPES buffered saline |
Kidney: Human | |
| |
| Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.
, Lipids Health Dis
10,
171, 2011 | |
| Cells: | Renal adipose derived cells |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Kidney: Human | |
| |
| Valente, M., Henrique, R., Costa, V., Jeronimo, C., Carvalho, F., Bastos, M., Guedes de Pinho, P. and Carvalho, M.: A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens.
, PLoS ONE
6,
e19337, 2011 | |
| Cells: | Renal tumor cells and proximal tubular epithelial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM/F12 |
Kidney: Human | |
| |
| Presnell, S., Bruce, A., Wallace, S., Choudhury, S., Genheimer, C., Cox, B., Guthrie, K., Werdin, E., Tatsumi-Ficht, P., Ilagan, R., Kelley, R., Rivera, E., Ludlow, J., Wagner, B., Jayo, M. and Bertram, T.: Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys.
, Tiss Eng
17,
261, 2011 | |
| Cells: | Renal cells |
| Enzyme(s) | Collagenase Type 4: 300 u/ml Neutral Protease: see reference |
| Medium | HBSS |
Kidney: Human | |
| |
| Johnson, D., Saunders, H., Johnson, F., Huq, S., Field, M., and Pollock, C.: Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor
, J Pharmacol Exp Ther 289,
535-42, 1999 | |
| Cells: | Renal proximal tubule and cortical fibroblasts |
| Enzyme(s) | Collagenase Type 2: 383 u/ml |
| Medium | DMEM/F-12 |
Kidney: Human | |
| |
| McAteer, J, Kempson, S., and Evan, A: Culture of Human Renal Cortex Epithelial Cells, J Tiss Cul Meth 13,
143, 1991 | |
| Cells: | Renal cortex |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Tissue Culture Grade Water |
Kidney: Human | |
| |
| Trifillis, A. and Kahng, M.: Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells
, In Vitro Cell Dev Biol 26,
441, 1990 | |
| Cells: | Papillary duct |
| Enzyme(s) | Collagenase: 400 u/ml |
| Medium | Eagle's MEM-HEPES buffer w/L-glutamine |
Kidney: Human | |
| |
| Heieren, M., van der Woude, F., and Balfour Jr., H.: Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells
, Proc Natl Acad Sci U S A 85,
1642, 1988 | |
| Cells: | Mesangial |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM/Ham's F-12 |
Kidney: Human | |
| |
| Yang, A., Gould-Kostka, J., and Oberley, T.: In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate
, In Vitro Cell Dev Biol 23 (1),
34, 1987 | |
| Cells: | Tubular |
| Enzyme(s) | Collagenase: 250 u/ml |
| Medium | PBS |
Kidney: Human | |
| |
| Trifillis, A., Regec, A., and Trump, B.: Isolation, Culture, and Charaterization of Human Renal Tubular Cells
, J Urol 133,
324, 1985 | |
| Cells: | Tubular |
| Enzyme(s) | Collagenase: 100 u/ml |
| Medium | Joklik's MEM |
Kidney: Human | |
| |
| Hemstreet, G., Enoch, P., and Pretlow, T.: Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification
, Cancer Res 40,
1043, 1980 | |
| Cells: | Malignant Stromal |
| Enzyme(s) | Papain: 0.009% |
| Medium | Sacks solution |
Kidney: Human | |
| |
| De Oca, H., and Malinin, T.: Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys
, J Clin Microbiol 2,
243, 1975 | |
| Cells: | Renal |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | See reference |
Kidney: Monkey | |
| |
| Melnick, J., and Wallis, C.: Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells
, Dev Biol 37,
77, 1976 | |
| Cells: | Kidney |
| Enzyme(s) | Trypsin: 0.01% - 0.00001% |
| Medium | Eagle's MEM |
Kidney: Mouse | |
| |
| Yuan, Y., Li, L., Zhu, L., Liu, F., Tang, X., Liao, G., Liu, J., Cheng, J., Chen, Y. and Lu, Y.: Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy.
, Stem Cells 38,
639-652, 2020 | |
| Cells: | Mactrophages |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Kidney: Mouse | |
| |
| Adam, M., Potter, A. and Potter, S.: Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development.
, Development
144,
3625-3632, 2017 | |
| Cells: | Kidney |
| Enzyme(s) | Deoxyribonuclease I: 125 u/ml Protease, Bacillus Licheniformis: 1% |
| Medium | DPBS |
Kidney: Mouse | |
| |
| DiRocco, D., Kobayashi, A., Taketo, M., McMahon, A. and Humphreys, B.: Wnt4/β-catenin Signaling in Medullary Kidney Myofibroblasts.
, J Am Soc Nephrol
24,
1399, 2013 | |
| Cells: | Collecting duct epithelial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Kidney: Mouse | |
| |
| Kabgani, N., Grigoleit, T., Schulte, K., Sechi, A., Sauer-Lehnen, S., Tag, C., Boor, P., Kuppe, C., Warsow, G., Schordan, S., Mostertz, J., Chilukoti, R., Homuth, G., Endlich, N., Tacke, F. and Weiskirchen, R.: Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin.
, PLoS ONE
7,
e34907, 2012 | |
| Cells: | Glomeruli |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | RPMI 1640 |
Kidney: Mouse | |
| |
| Sedrakyan, S., Da Sacco, S., Milanesi, A., Shiri, L., Petrosyan, A., Varimezova, R., Warburton, D., Lemley, K., De Filippo, R. and Perin, L.: Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis.
, J Am Soc Nephrol
23,
661-73, 2012 | |
| Cells: | Glomeruli |
| Enzyme(s) | Collagenase Type 1: 0.1% Deoxyribonuclease I: 100 u/ml |
| Medium | DMEM |
Kidney: Mouse | |
| |
| Park, J., Pasupulati, R., Feldkamp, T., Roeser, N. and Weinberg, J.: Cyclophilin D and the Mitochondrial Permeability Transition in Kidney Proximal Tubules after Hypoxic and Ischemic Injury.
, Am J Physiol/Renal 301,
F134, 2011 | |
| Cells: | Proximal tubule |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | See reference |
Kidney: Mouse | |
| |
| Brown, A., Blank, U., Adams, D., Karolak, M., Fetting, J., Hill, B. and Oxburgh, L.: Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney.
, J Vis Exp
50,
e2555, 2011 | |
| Cells: | Kidney |
| Enzyme(s) | Collagenase Type 1: 0.25% Pancreatin: 1.0% Deoxyribonuclease I: 1 u/ml |
| Medium | HBSS |
Kidney: Mouse | |
| |
| Huang, Y., Johnston, P., Zhang, B., Zakari, A., Chowdhry, T., Smith, R., Marban, E., Rabb, H. and Womer, K.: Kidney-Derived Stromal Cells Modulate Dendritic and T Cell Responses.
, J Am Soc Nephrol
20,
831-41, 2009 | |
| Cells: | Kidney sphere-derived |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | Iscove's Modified Dulbecco's |
Kidney: Mouse | |
| |
| Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress.
, Oxid Med Cell Longev
1,
33, 2008 | |
| Cells: | Renal tubular epithelial |
| Enzyme(s) | Collagenase: 200 u/ml Soybean Trypsin Inhibitor: see reference |
| Medium | HBSS |
Kidney: Mouse | |
| |
| Wright, J., Morales, M., Sousa-Menzes, J., Ornellas, D., Sipes, J., Cui, Y., Cui, I., Hulamm, P., Cebotaru, V., Cebotaru, L., Guggino, W. and Guggino, S.: Transcriptional Adaptation to Clcn5 Knockout in Proximal Tubules of Mouse Kidney.
, Physiol Genomics
33,
341, 2008 | |
| Cells: | Proximal tubule |
| Enzyme(s) | Collagenase Type 2: 0.013% |
| Medium | see reference |
Kidney: Mouse | |
| |
| Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis.
, Am J Physiol Cell Physiol
292,
C2070, 2007 | |
| Cells: | Endothelial kidney |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Kidney: Mouse | |
| |
| Syal Ashu, Schiavi Susan, Chakravarty Sumana, Dwarakanath Vangipuram, Quigley Raymond, Baum Michel: Fibroblast growth factor-23 increases mouse PGE2 production in vivo and in vitro
, Am J Physiol/Renal 290,
F450-5, 2006 | |
| Cells: | Cortex, proximal tubule |
| Enzyme(s) | Collagenase: 0.15% |
| Medium | DMEM |
Kidney: Mouse | |
| |
| Plotkin, M. and Goligorsky, M.: Mesenchymal Cells from Adult Kidney Support Angiogenesis and Differentiate into Multiple Interstitial Cell Types including Erythropoietin-Producing Fibroblasts.
, Am J Physiol Renal Physiol
291,
F902-12, 2006 | |
| Cells: | Kidney mesenchymal |
| Enzyme(s) | Collagenase: 250 u/ml Neutral Protease: 33.3 u/ml |
| Medium | DMEM/F12 |
Kidney: Mouse | |
| |
| Dekel, B., Zangi, L., Shezen, E., Reich-Zeliger, S., Eventov-Friedman, S., Katchman, H., Jacob-Hirsch, J., Amariglio, N., Rechavi, G., Margalit, R. and Reisner, Y.: Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney.
, J Am Soc Nephrol
17,
3300, 2006 | |
| Cells: | Kidney |
| Enzyme(s) | Collagenase Type 4: 0.075% |
| Medium | DMEM |
Kidney: Mouse | |
| |
| Cunningham, R., Xiaofei, E., Steplock, D., Shenolikar, S. and Weinman, E.: Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media.
, Am J Physiol Renal Physiol
289,
F933, 2005 | |
| Cells: | Renal proximal tubule cells |
| Enzyme(s) | Collagenase Type 2: 0.1% Soybean Trypsin Inhibitor: 0.25% |
| Medium | DMEM/F-12 |
Kidney: Mouse | |
| |
| Sindic, A., Velic, A., Basoglu, C., Hirsch, J., Edemir, B., Kuhn, M. and Schlatter, E.: Uroguanylin and Guanylin Regulate Transport of Mouse Cortical Collecting Duct Independent of Guanylate Cyclase C.
, Kidney Int
68,
1008, 2005 | |
| Cells: | Cortical collecting duct |
| Enzyme(s) | Collagenase: 54-178 u/ml Protease: see reference |
| Medium | MEM |
Kidney: Mouse | |
| |
| Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE.: Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis
, J Biol Chem 277(30),
27535, 2002 | |
| Cells: | Glomerular mesangial cells |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | RPMI 1640 |
Kidney: Mouse | |
| |
| Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation
, Mol Biol Cell
10,
1569-79, 1999 | |
| Cells: | Fibroblasts, mesangial, smooth muscle |
| Enzyme(s) | Trypsin: 0.25% Collagenase: see reference Soybean Trypsin Inhibitor: 0.05% |
| Medium | DMEM |
Kidney: Mouse | |
| |
| Taub, M: Renal Tubule Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley & Sons, Ltd.,
14B:6.1, 1995 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 4: 1% |
| Medium | DMEM /F-12 |
Kidney: Mouse | |
| |
| Haverty, T., Kelly, C., Hines, W., Amenta, P., Watanabe, M., Harper, R., Kefalides, N., and Neilson, E.: Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis
, J Cell Biol
107,
1359, 1988 | |
| Cells: | Proximal tubular epithelial |
| Enzyme(s) | Deoxyribonuclease I: 15 µg/ml |
| Medium | RPMI 1640 |
Kidney: Porcine | |
| |
| Heussner, A. and Dietrich, D.: Primary Porcine Proximal Tubular Cells as an Alternative to Human Primary Renal Cells in Vitro: an Initial Characterization.
, BMC Cell Biol 14,
55, 2013 | |
| Cells: | Kidney |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Kidney: Rabbit | |
| |
| Nowak, G. and Bakajsova, D.: Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes.
, J Vis Exp
71,
e4301, 2013 | |
| Cells: | Renal proximal tubular |
| Enzyme(s) | Collagenase Type 1: 60 u/ml |
| Medium | DMEM/F12 |
Kidney: Rabbit | |
| |
| Taub, M.: Primary Kidney Proximal Tubule Cells.
, Methods Mol Biol 290,
231, 2005 | |
| Cells: | Proximal tubule cells |
| Enzyme(s) | Collagenase Type 4: 0.005% Soybean Trypsin Inhibitor: 0.00025% |
| Medium | DMEM/F-12 |
Kidney: Rabbit | |
| |
| Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments
, Am J Physiol 273,
F650, 1997 | |
| Cells: | Nephron segments |
| Enzyme(s) | Collagenase Type 2: 0.05% Deoxyribonuclease I: 50 u/ml |
| Medium | Eagle's MEM |
Kidney: Rabbit | |
| |
| Naray-Fejes-Toth, A., and Fejes-Toth, G.: Immunoelection and Culture of Cortical Collecting Duct Cells, J Tiss Cul Meth 13,
179, 1991 | |
| Cells: | Duct |
| Enzyme(s) | Soybean Trypsin Inhibitor: 0.025% |
| Medium | Hank's Solution with calcium and HEPES |
Kidney: Rabbit | |
| |
| Rodeheaver, D., Aleo, M., and Schnellmann, R.: Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation
, In Vitro Cell Dev Biol 26,
898, 1990 | |
| Cells: | Renal proximal tubules |
| Enzyme(s) | Deoxyribonuclease I: 70 u/ml |
| Medium | Modified DME-F12 |
Kidney: Rabbit | |
| |
| Allen, M., Nakao, A., Sonnenburg, W., Burnatowska-Hledin, M., Speilman, W., and Smith, W.: Immunodissection of Cortical and Medullary Thick Ascending Limb Cells From Rabbit Kidney
, Am J Physiol 255,
F704, 1988 | |
| Cells: | MTALH cells RCCT cells |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | DMEM |
Kidney: Rabbit | |
| |
| Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture
, Am J Physiol 255,
F1160, 1988 | |
| Cells: | Papillary duct |
| Enzyme(s) | Hyaluronidase: 0.2% |
| Medium | Keri's buffer HEPES buffered saline |
Kidney: Rabbit | |
| |
| Grenier, F., and Smith, W.: Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae, Prostaglandins 16,
759, 1978 | |
| Cells: | Collecting tubule |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Kreb's Ringer buffer |
Kidney: Rabbit | |
| |
| Dworzack, D., and Grantham, J.: Preparation of Renal Papillary Collecting Duct Cells for Study
, Nat Neurosci 8,
191, 1975 | |
| Cells: | Papillary collecting duct |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | (see reference) |
Kidney: Rat | |
| |
| Gupta, A., Jadhav, S., Tripathy, N. and Nityanand, S.: Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects.
, PLoS ONE
10,
e0131057, 2015 | |
| Cells: | Fetal kidney |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | MEM |
Kidney: Rat | |
| |
| Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.
, Lipids Health Dis
10,
171, 2011 | |
| Cells: | Renal adipose derived cells |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Kidney: Rat | |
| |
| Panico, C., Luo, Z., Damiano, S., Artigiano, F., Gill, P. and Welch, W.: Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3.
, Hypertension 54,
1291, 2009 | |
| Cells: | Proximal tubule |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | see reference |
Kidney: Rat | |
| |
| Silva, G., Beierwaltes, W., and Garvin, J.: Extracellular ATP stimulates NO production in rat thick ascending limb
, Hypertension 47,
563-7, 2006 | |
| Cells: | Medullary thick ascending limb |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HEPES-saline |
Kidney: Rat | |
| |
| Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C: Kidney oxygen consumption, carbonic anhydrase, and proton secretion
, Am J Physiol/Renal 290,
F1009-15, 2006 | |
| Cells: | Renal proximal tubules |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM/F12 |
Kidney: Rat | |
| |
| Deng, A., Miracle, C., Suarez, J., Lortie, M., Satriano, J., Thomson, S., Munger, K. and Blantz, R.: Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II.
, Kidney Int
68,
723, 2005 | |
| Cells: | Renal proximal tubules |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM/F-12 |
Kidney: Rat | |
| |
| Arystarkhova, E., Wetzel R., and Sweadner K.: Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney
, Am J Physiol/Renal 282(3),
F393, 2002 | |
| Cells: | Cortical tubule cells |
| Enzyme(s) | Collagenase: 0.6% |
| Medium | DMEM |
Kidney: Rat | |
| |
| Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B: Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose
, Kidney Int Suppl 61,
1605-16, 2002 | |
| Cells: | Glomerular mesangial cells |
| Enzyme(s) | Collagenase Type 1: 250 u/ml |
| Medium | DMEM |
Kidney: Rat | |
| |
| Valencia L., Bidet M., Martial S., Sanchez E., Melendez E., Tauc M., Poujeol C., Martin D., Namorado M., Reyes J., and Poujeol P.: Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture
, Am J Physiol Cell Physiol
280(5),
C1193, 2001 | |
| Cells: | Cortical collection duct |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | DMEM/Ham's F12 |
Kidney: Rat | |
| |
| Li, N., Teggatz, E., Li, P., Allaire, R., and Zou, A.: Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels
, Microvasc Res 60,
149, 2000 | |
| Cells: | Microvessels |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | PSS |
Kidney: Rat | |
| |
| Mattson D., and Wu F.: Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature
, Hypertension 35,
337, 2000 | |
| Cells: | Renal |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | see reference |
Kidney: Rat | |
| |
| Miyata, N., Park, F., Li, X., and Cowley, A.: Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney
, Am J Physiol 277,
F437, 1999 | |
| Cells: | Renal tubules |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | see reference |
Kidney: Rat | |
| |
| Amiri F, Garcia R: Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells
, Am J Physiol 276,
F691-9, 1999 | |
| Cells: | Glomerular mesangial cells |
| Enzyme(s) | Collagenase Type 1: 250 u/ml |
| Medium | DMEM |
Kidney: Rat | |
| |
| Eitle E, Hiranyachattada S, Wang H, Harris PJ: Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney
, Am J Physiol 274,
C1075-80, 1998 | |
| Cells: | Proximal tubule suspensions |
| Enzyme(s) | Collagenase Type 4: 0.1% Pronase E: 2 u/ml |
| Medium | HEPES buffer |
Kidney: Rat | |
| |
| Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments
, Am J Physiol 273,
F650, 1997 | |
| Cells: | Nephron segments |
| Enzyme(s) | Collagenase Type 2: 0.05% Deoxyribonuclease I: 50 u/ml |
| Medium | Eagle's MEM |
Kidney: Rat | |
| |
| Ishikawa, S., Kusaka, I., Higashiyama, M., Nagasaka, S., Saito, T., Honda, K., and Saito, T.: Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein
, Nat Neurosci 50,
1506, 1996 | |
| Cells: | Renal |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | DMEM |
Kidney: Rat | |
| |
| Elliget, K., and Trump, B.: Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury
, In Vitro Cell Dev Biol 27,
739, 1991 | |
| Cells: | Epithelial Proximal tubule |
| Enzyme(s) | Protease: 0.1% |
| Medium | HBSS/CMF |
Kidney: Rat | |
| |
| Brion, L., Schwartz, J., Lachman, H., Zavilowitz, B., and Schwartz, G.: Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells
, Am J Physiol 257,
F486, 1989 | |
| Cells: | Inner medullary collecting duct Papillae |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | PBS |
Kidney: Rat | |
| |
| Wang, J., Kester, M., and Dunn, M.: The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells
, Biochim Biophys Acta 969,
217, 1988 | |
| Cells: | Glomerular mesangial |
| Enzyme(s) | Collagenase: 0.025% |
| Medium | RPMI 1640 |
Kidney: Rat | |
| |
| Barlet-Bas, C., Khadouri, C., Marsey, S., and Doucet, A.: Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine
, Proc Natl Acad Sci U S A 85,
1707, 1988 | |
| Cells: | Renal target |
| Enzyme(s) | Collagenase: 1.0% (also 0.7%) |
| Medium | Eagle's MEM |
Kidney: Rat | |
| |
| Gesek, F., Wolff, D., and Strandhov, J.: Improved Separation Method for Rat Proximal and Distal Renal Tubules
, Am J Physiol 253,
F358, 1987 | |
| Cells: | Proximal tubules |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Krebs-Henseleit buffer |
Kidney: Rat | |
| |
| Vinay, P., Gougoux, A., and Lemieux, G.: Isolation of a Pure Suspension of Rat Proximal Tubules
, Am J Physiol 241,
F403, 1981 | |
| Cells: | Proximal tubules |
| Enzyme(s) | Collagenase: 0.15 % |
| Medium | Krebs Henseleit solution |
Kidney: Rat | |
| |
| Wallach, D., Anderson, W., and Pastan, I.: Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin
, J Biol Chem 253,
24, 1978 | |
| Cells: | Fibroblasts Kidney |
| Enzyme(s) | Trypsin: up to 0.25% |
| Medium | Dulbecco-Vogt MEM |
Liver: Avian | |
| |
| Else, P., Brand, M., Turner, N. and Hulbert, A: Respiration Rate of Hepatocytes Varies With Body Mass in Birds
, J Exp Biol
207,
2305, 2004 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | see reference |
Liver: Avian | |
| |
| Lee J., Culvenor J., Angus P., Smallwood R., Nicoll A., and Locarnini S.: Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells
, J Virol 75(16),
7651, 2001 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% Hyaluronidase: 0.05% |
| Medium | DMEM/ F12 |
Liver: Canine | |
| |
| Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies
, In Vitro 17,
935, 1981 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 90 u/ml |
| Medium | CF EGTA perfusate |
Liver: Canine | |
| |
| Vickrey, H., Ramon, J., and McCann, D.: Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions
, In Vitro 15,
120, 1979 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS, CMF |
Liver: Chicken | |
| |
| Fraslin, J., Touquette, L., Douaire, M., Menezo, Y., Guillemot, J., and Mallard, J.: Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture
, In Vitro Cell Dev Biol 28,
615, 1992 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.02% |
| Medium | HEPES, CF |
Liver: Chicken | |
| |
| Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars
, J Biol Chem 254 (21),
10830, 1979 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 6000 units Collagenase Type 3 & 4: |
| Medium | HEPES |
Liver: Chicken | |
| |
| Tarlow, D., Watkins, P., Reed, R., Miller, R., Zwergel, E., and Lane, M.: Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture
, J Cell Biol
73,
332, 1974 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Deoxyribonuclease I: 0.00125% |
| Medium | PBS |
Liver: Chicken | |
| |
| Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation
, Exp Cell Res
30,
257, 1963 | |
| Cells: | Heart Liver |
| Enzyme(s) | Trypsin: 3.0% |
| Medium | Tyrode's solution, CMF |
Liver: Chicken | |
| |
| Rinaldini, L.: An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells
, Exp Cell Res
16,
477, 1959 | |
| Cells: | Various tissues (heart, liver, skeletal, cardiac) |
| Enzyme(s) | Trypsin: various grades |
| Medium | CMF Tyrode's solution |
Liver: Equine | |
| |
| Bakala A., Karlik W., and Wiechetek M.: Preparation of Equine Isolated Hepatocytes
, Toxicol In Vitro
17(5-6),
615, 2003 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | HBSS |
Liver: Fish | |
| |
| Blair, J.B., Miller, M.R., Pack, D., Barnes, R., Teh, S.J. and Hinton, D.E.: Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions
, In Vitro Cell Dev Biol 26,
237, 1990 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.045% |
| Medium | Kreb's-Ringer bicarbonate buffer, CF |
Liver: Fish | |
| |
| Lipsky, M., Sheridan, T., Bennett, R., and May, E.: Comparison of Trout Hepatocyte Culture on Different Substrates, In Vitro Cell Dev Biol 22,
360, 1986 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.045% |
| Medium | HBSS with 0.05M HEPES |
Liver: Fish | |
| |
| Klaunig, J., Ruch, R., and Goldblah, P.: Trout Hepatocyte Culture: Isolation and Primary Culture
, In Vitro Cell Dev Biol 21,
221, 1985 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 100 u/ml |
| Medium | HBSS/CMF |
Liver: Fish | |
| |
| Bailey, G., Taylor, M., and Selivonchick, D.: Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner
, Carcinogenesis
3,
511, 1982 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Hyaluronidase: 0.08% |
| Medium | See reference |
Liver: Fish | |
| |
| Klaunig, J.: Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies, Natl Cancer Inst Monogr 65,
163, 1981 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 100u/ml |
| Medium | HBSS |
Liver: Guinea-Pig | |
| |
| Arinze, I. and Kawai, Y.: Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis
, Arch Biochem Biophys
225,
196, 1983 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: |
| Medium | Kreb's Ringer bicarbonate buffer |
Liver: Guinea-Pig | |
| |
| Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies
, In Vitro 17,
935, 1981 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 90 u/ml |
| Medium | CF EGTA perfusate |
Liver: Human | |
| |
| Reuschel, E, Jilg, W., Seelbach-Goebel, B. and Demi, L.: Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes
, PLoS ONE
,
, 2019 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | HBSS |
Liver: Human | |
| |
| Werner, M., Driftmann, S., Kleinehr, K., Kaiser, G., Mathe, Z., Treckmann, J., Paul, A., Skibbe, K., Timm, J., Canbay, A., Gerken, G., Schlaak, J. and Broering, R.: All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells.
, PLoS ONE
10,
e0138655, 2015 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.06% |
| Medium | DMEM |
Liver: Human | |
| |
| Pfeiffer, E., Kegel, V., Zeilinger, K., Hengstler, J., Nussler, A., Seehofer, D. and Damm, G.: Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells.
, Exp Biol Med (Maywood)
240,
645-56, 2015 | |
| Cells: | Hepatocytes and non-parenchymal cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Liver: Human | |
| |
| Lee, S., Schelcher, C., Demmel, M., Hauner, M. and Thasler, W.: Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure.
, J Vis Exp
,
, 2013 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.1-0.15% |
| Medium | HEPES |
Liver: Human | |
| |
| Vatakis, D., Bristol, G., Kim, S., Levin, B., Liu, W., Radu, C., Kitchen, S. and Zack, J.: Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model.
, J Vis Exp
70,
e4181, 2012 | |
| Cells: | Liver hematopoietic |
| Enzyme(s) | Collagenase Type 4: 0.1% Hyaluronidase: 0.1% Deoxyribonuclease I: 2 u/ml |
| Medium | RPMI |
Liver: Human | |
| |
| Torok, E., Lutgehetmann, M., Bierwolf, J., Melbeck, S., Dullmann, J., Nashan, B., Ma, P. and Pollok, J.: Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering.
, Liver Transpl 17,
104, 2011 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | Williams E |
Liver: Human | |
| |
| Wauthier, E., Schmelzer, E., Turner, W., Zhang, L., LeCluyse, E., Ruiz, J., Turner, R., Furth, M., Kubota, H., Lozoya, O., Barbier, C., McClelland, R., Yao, H., Moss, N., Bruce, A., Ludlow, J. and Reid, L.: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance.
, Methods Cell Biol 86,
137, 2008 | |
| Cells: | Hepatic stem cells and heptoblasts |
| Enzyme(s) | Collagenase Type 4: 0.014-0.06% |
| Medium | various |
Liver: Human | |
| |
| Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC, and Maurel P: Human hepatocyte culture
, Methods Mol Biol 320,
283, 2006 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HEPES |
Liver: Human | |
| |
| Hughes, R., Mitry, R., Dhawan, A., Lehec, S., Girlanda, R., Rela, M., Heaton, N., and Muiesan, P.: Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation
, Liver Transpl 12,
713, 2006 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | EBSS |
Liver: Human | |
| |
| Hussain, S., Strom, S., Kirby, M., Burns, S., Langemeijer, S., Ueda, T., Hsieh, M. and Tisdale, J.: Side Population Cells Derived from Adult Human Liver Generate Hepatocyte-Like Cells In Vitro.
, Dig Dis Sci
50,
1755, 2005 | |
| Cells: | Hepatic side population |
| Enzyme(s) | Collagenase: 0.02-0.05% |
| Medium | HBSS |
Liver: Human | |
| |
| Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R, and Gupta S.:
Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications
, Liver Int
24,
361, 2004 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.6% |
| Medium | RPMI 1640 |
Liver: Human | |
| |
| Duanmu Z., Locke D., Smigelski J., Wu W., Dahn M., Falany C., Kocarek T., and Runge-Morris M.: Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes
, Drug Metab Dispos 30(9),
997, 2002 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | Williams E |
Liver: Human | |
| |
| Malhi, H., Irani, A., Gagandeep, S. and Gupta, S.: Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes.
, J Cell Sci
115,
2679, 2002 | |
| Cells: | Epithelial progenitor |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Liver: Human | |
| |
| Dandri, M., Burda, M., Torok, E., Pollok, J., Iwanska, A., Sommer, G., et al.: Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus
, Hepatol 33,
981, 2001 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | Leffert's buffer |
Liver: Human | |
| |
| Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers
, Drug Metab Dispos 28,
1321-6, 2000 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Williams E |
Liver: Human | |
| |
| Parzefall, W., Erber, E., Sedivy, R., and Schulte-Hermann, R.: Testing for Induction of DNA Synthesis in Human Hepatocyte Primary Cultures by Rat Liver Tumor Promoters
, Cancer Res 51,
1143, 1991 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.025% |
| Medium | Williams E |
Liver: Human | |
| |
| Gomez-Lechon, M., Lopez, P., Donato, T., Montoya, A., Larrauri, A., Gimenez, P., Trullenque, R., Fabra, R., and Castell, J.: Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo
, In Vitro Cell Dev Biol 26,
67, 1990 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HEPES buffer (see reference) |
Liver: Human | |
| |
| Begue, J., Baffet, G., Campion, J., and Guillouzo, A.: Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3
, Biol Cell 63,
327, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HEPES buffer |
Liver: Human | |
| |
| LeBot, M., Begue, J., Kernaleguen, D., Robert, J., Ratanasavanh, D., Airiau, J., Riche, C., and Guillouzo, A.: Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes
, Biochem Pharmacol 37 (20),
3877, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% & 0.025% |
| Medium | HEPES (see reference) |
Liver: Human | |
| |
| Gugen-Guillouzo, C., Campion, J., Brissot, P., Glaise, D., Launois, B., Bourel, M., and Guillouzo, A.: High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver
, Cell Biol Int Rep 6 (6),
625, 1982 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HEPES |
Liver: Human | |
| |
| Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies
, In Vitro 17,
935, 1981 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 90 u/ml |
| Medium | CF EGTA perfusate |
Liver: Human | |
| |
| Kaighn, M.: Human Liver Cells,Tissue Culture Methods / Applications,(Kruse, P., Patterson, M. eds), ,
54, 1973 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS, CMF |
Liver: Monkey | |
| |
| Weber, A., Groyer-Picard, M.and Dagher, I.: Hepatocyte Transplantation Techniques: Large Animal Models.
, Methods Mol Biol 481,
83, 2009 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 129 u/ml |
| Medium | DMEM/F12 |
Liver: Monkey | |
| |
| Ulrich, R., Aspar, D., Cramer, C., Kletzien, R., and Ginsberg, L.: Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis)
, In Vitro Cell Dev Biol 26,
815, 1990 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Trypsin: 160 u/ml |
| Medium | HEPES buffer |
Liver: Mouse | |
| |
| Cabral, F., Miller, C., Kudrna, K., Hass, B., Daubendiek, J., Kellar, B. and Harris, E.: Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion.
, J Vis Exp
,
, 2018 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | DMEM |
Liver: Mouse | |
| |
| Sewing, S., Boess, F., Moisan, A., Bertinetti-Lapatki, C., Minz, T., Hedtjaern, M., Tessier, Y., Schuler, F., Singer, T. and Roth, A.: Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs.
, PLoS ONE
11,
e0159431, 2016 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.2 u/ml |
| Medium | William's Medium E |
Liver: Mouse | |
| |
| Weerasinghe, S., Park, M., Portney, D. and Omary, M.: Mouse Genetic Background Contributes to Hepatocyte Susceptibility to Fas-mediated Apoptosis.
, Mol Biol Cell
27,
3005-3012, 2016 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 150 u/ml |
| Medium | PBS |
Liver: Mouse | |
| |
| Bigorgne, A., John, B., Ebrahimkhani, M., Shimizu-Albergine, M., Campbell, J. and Crispe, I.: TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota.
, PLoS ONE
11,
e0151063, 2016 | |
| Cells: | Hepatic |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | HBSS |
Liver: Mouse | |
| |
| Mederacke, I., Dapito, D., Affo, S., Uchinami, H. and Schwabe, R.: High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers.
, Nat Protoc
10,
305-15, 2015 | |
| Cells: | Hepatic stellate |
| Enzyme(s) | Collagenase Type 4: 0.05% Pronase: 0.04% |
| Medium | DMEM |
Liver: Mouse | |
| |
| Guenthner, C., Luitje, M., Pyle, L., Molyneux, P., Yu, J., Li, A., Leise, T. and Harrington, M.: Circadian Rhythms of Per2::Luc in Individual Primary Mouse Hepatocytes and Cultures.
, PLoS ONE
9,
e87573, 2014 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 3: 100 u/ml Collagenase Type 4: 100 u/ml |
| Medium | DMEM |
Liver: Mouse | |
| |
| Sin, Y., Ballantyne, L., Mukherjee, K., St Amand, T., Kyriakopoulou, L., Schulze, A. and Funk, C.: Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism.
, PLoS ONE
8,
e80001, 2013 | |
| Cells: | hepatocytes |
| Enzyme(s) | Collagenase Type 4: 100 u/ml |
| Medium | DMEM |
Liver: Mouse | |
| |
| Perepelyuk, M., Terajima, M., Wang, A., Georges, P., Janmey, P., Yamauchi, M. and Wells, R.: Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury.
, Am J Physiol Gastrointest Liver Physiol
304,
G605, 2013 | |
| Cells: | Hepatocytes and sinusoidal endothelial |
| Enzyme(s) | Collagenase Type 4: 0.05% Deoxyribonuclease I: 0.003% |
| Medium | HBSS |
Liver: Mouse | |
| |
| Severgnini, M., Sherman, J., Sehgal, A., Jayaprakash, N., Aubin, J., Wang, G., Zhang, L., Peng, C., Yucius, K., Butler, J. and Fitzgerald, K.: A Rapid Two-Step Method for Isolation of Functional Primary Mouse Hepatocytes: Cell Characterization and Asialoglycoprotein Receptor Based Assay Development.
, Cytotechnology
64,
187-95, 2012 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.08% |
| Medium | DMEM |
Liver: Mouse | |
| |
| Brundert, M., Heeren, J., Merkel, M., Carambia, A., Herkel, J., Groitl, P., Dobner, T., Ramakrishnan, R., Moore, K. and Rinninger, F.: Scavenger Receptor CD36 Mediates Uptake of High Density Lipoproteins in Mice and by Cultured Cells.
, J Lipid Res 52,
745, 2011 | |
| Cells: | Hepatocytes & non-parenchymal liver cells |
| Enzyme(s) | Collagenase: 0.03-0.05% |
| Medium | DMEM |
Liver: Mouse | |
| |
| Kang, H., Okamoto, K., Kim, Y., Takeda, Y., Bortner, C., Dang, H., Wada, T., Xie, W., Yang, X., Liao, G. and Jetten, A.: Nuclear Orphan Receptor TAK1/TR4-Deficient Mice are Protected Against Obesity-Linked Inflammation, Hepatic Steatosis, and Insulin Resistance.
, Diabetes 60,
177, 2011 | |
| Cells: | Hepatocytes |
| Enzyme(s) | HIS kit: with modifications |
| Medium | DMEM |
Liver: Mouse | |
| |
| Holl, D., Kuckenberg, P., Woynecki, T., Egert, A., Becker, A., Huss, S., Stabenow, D., Zimmer, A., Knolle, P., Tolba, R., Fischer, H. and Schorle, H.: Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia.
, PLoS ONE
6,
e22034, 2011 | |
| Cells: | Hepatacytes |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | Williams E |
Liver: Mouse | |
| |
| Rountree, C., Ding, W., Dang, H., Vankirk, C. and Crooks, G.: Isolation of CD133+ Liver Stem Cells for Clonal Expansion.
, J Vis Exp
56,
e3183, 2011 | |
| Cells: | CD133+ stem |
| Enzyme(s) | Collagenase Type 4: 0.05% Pronase: 0.05% Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F12 |
Liver: Mouse | |
| |
| Chung, S., Timmins, J., Duong, M., Degirolamo, C., Rong, S., Sawyer, J., Singaraja, R., Hayden, M., Maeda, N., Rudel, L., Shelness, G. and Parks, J.: Targeted Deletion of Hepatocyte ABCA1 Leads to VLDL Triglyceride Over-Production and LDL Hypercatabolism
, J Biol Chem 285,
12197, 2010 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.03% |
| Medium | Williams E |
Liver: Mouse | |
| |
| Bosschaerts, T., Guilliams, M., Stijlemans, B., Morias, Y., Engel, D., Tacke, F., Herin, M., De Baetselier, P. and Beschin, A.: Tip-DC Development During Parasitic Infection is Regulated by IL-10 and Requires CCL2/CCR2, IFN-gamma and MyD88 Signaling.
, PLoS Pathog 6,
e1001045, 2010 | |
| Cells: | Liver non-parenchymal |
| Enzyme(s) | Collagenase Type 3: 100 u/ml |
| Medium | HBSS |
Liver: Mouse | |
| |
| Shmarakov, I., Fleshman, M., D'Ambrosio, D., Piantedosi, R., Riedl, K., Schwartz, S., Curley, R., von Lintig, J., Rubin, L., Harrison, E. and Blaner, W.: Hepatic Stellate Cells are an Important Cellular Site for β-Carotene Conversion to Retinoid.
, Arch Biochem Biophys
504,
3-10, 2010 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | HBSS |
Liver: Mouse | |
| |
| Mathijs, K., Kienhuis, A., Brauers, K. J, Jennen, D. , Lahoz, A., Kleinjans, J. and van Delft, J.: Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes.
, Drug Metab Dispos 37,
1305, 2009 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | HBSS |
Liver: Mouse | |
| |
| Oliva, J., Bardag-Gorce, F., French, B., Li, J., McPhaul, L., Amidi, F., Dedes, J., Habibi, A., Nguyen, S. and French, S.: Fat10 is an Epigenetic Marker for Liver Preneoplasia in a Drug-Primed Mouse Model of Tumorigenesis.
, Exp Mol Pathol 84,
102, 2008 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 100 u/ml Elastase: 0.1 u/ml |
| Medium | Williams E |
Liver: Mouse | |
| |
| Follenzi, A., Benten, D., Novikoff, P., Faulkner, L., Raut, S. and Gupta, S.: Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice.
, J Clin Invest 118,
935, 2008 | |
| Cells: | Liver endothelial |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Liver: Mouse | |
| |
| Beldi, G., Wu, Y., Sun, X., Imai, M., Enjyoji, K., Csizmadia, E., Candinas, D., Erb, L. and Robson, S.: Regulated Catalysis of Extracellular Nucleotides by Vascular CD39/ENTPD1 is Required for Liver Regeneration.
, Gastroenterol 135,
1751, 2008 | |
| Cells: | Liver sinusoidal endothelial cells |
| Enzyme(s) | Collagenase Type 1: 0.05% Neutral Protease: 0.025% |
| Medium | HEPES |
Liver: Mouse | |
| |
| Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse
, Stem Cells 24,
322-32, 2006 | |
| Cells: | Liver epithelial progenitor cells |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.05% |
| Medium | DMEM |
Liver: Mouse | |
| |
| Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice
, Hepatol 42,
140-8, 2005 | |
| Cells: | Liver sinusodial endothelial |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Liver: Mouse | |
| |
| Jiang Guoqiang, Li Zhihua, Liu Franklin, Ellsworth Kenneth, Dallas-Yang Qing, Wu Margaret, Ronan John, Esau Christine, Murphy Cain, Szalkowski Deborah, Bergeron Raynald, Doebber Thomas, Zhang Bei: Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1
, J Clin Invest 115,
1030-8, 2005 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.033% |
| Medium | Leffert's buffer |
Liver: Mouse | |
| |
| Kotton, D., Fabian, A. and Mulligan, R.: A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity.
, Blood 106,
1574, 2005 | |
| Cells: | Liver derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% Neutral Protease: 2.4 u/ml |
| Medium | HBSS |
Liver: Mouse | |
| |
| Hatano E, Brenner DA: Akt protects mouse hepatocytes from TNF-alpha- and Fas-mediated apoptosis through NK-kappa B activation.
, Am J Physiol/Gastro 282,
G1357, 2002 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.04% |
| Medium | Waymouth's medium |
Liver: Mouse | |
| |
| Sazani P., Gemignani F., Kang S., Maier M., Manoharan M., Persmark M., Bortner D., and Kole R.: Systemically Delivered Antisense Oligomers Up-regulate Gene Expression in Mouse Tissues
, Nat Biotechnol
20,
1228, 2002 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.053% |
| Medium | DMEM/ F-12 |
Liver: Mouse | |
| |
| Lingohr Melissa K, Bull Richard J, Kato-Weinstein Junko, Thrall Brian D: Dichloroacetate stimulates glycogen accumulation in primary hepatocytes through an insulin-independent mechanism
, Toxicol Sci 68,
508-15, 2002 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 100 u/ml |
| Medium | HBSS |
Liver: Mouse | |
| |
| Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers
, Drug Metab Dispos 28,
1321-6, 2000 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Williams E |
Liver: Mouse | |
| |
| Angele MK, Knoferl MW, Schwacha MG, Ayala A, Cioffi WG, Bland KI, and Chaudry IH.: Sex steroids regulate pro- and anti-inflammatory cytokine release by macrophages after trauma-hemorrhage
, Am J Physiol 277,
C35, 1999 | |
| Cells: | Kupffer cells |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | HBSS |
Liver: Mouse | |
| |
| Ling W, Lougheed M, Suzuki H, Buchan A, Kodama T, Steinbrecher UP: Oxidized or acetylated low density lipoproteins are rapidly cleared by the liver in mice with disruption of the scavenger receptor class A type I/II gene
, J Clin Invest 100,
244-52, 1997 | |
| Cells: | Nonparenchymal liver |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | Hanks |
Liver: Mouse | |
| |
| Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver
, In Vitro 15,
910, 1979 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 0.10% |
| Medium | DMEM |
Liver: Mouse | |
| |
| Crisp, D., and Pogson, C.: Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver
, Biochem J
126,
1009, 1972 | |
| Cells: | Parenchymal and non-parenchymal |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Hank's w/ Insulin, CMF |
Liver: Porcine | |
| |
| Meng, F., Chen, Z., Han, M., Hu, X., He, X., Liu, Y., He, W., Huang, W., Guo, H. and Zhou, P.: Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination.
, World J Gastroenterol
16,
1660, 2010 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% Neutral Protease: 0.84% Deoxyribonuclease I: see reference |
| Medium | Williams E |
Liver: Porcine | |
| |
| Terner, M., Gilmore, W.J., Lou, Y. and Squires, E.J.: The Role of CYP2A and CYP2E1 in the Metabolism of 3-Methylindole in Primary Cultured Porcine Hepatocytes.
, Drug Metab Dispos 34,
848, 2006 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.07% |
| Medium | Williams E |
Liver: Porcine | |
| |
| Li, J, Li, L., Chao, H., Yang, Q., Liu, X., Sheng, J., Yu, H. and Huang, J: Isolation and Short Term Cultivation of Swine Hepatocytes for Bioartificial Liver Support System.
, Hepatobiliary Pancreat Dis Int
4,
249, 2005 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 125 u/ml |
| Medium | Williams E |
Liver: Porcine | |
| |
| Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich
, World J Gastroenterol
10,
699, 2004 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | RPMI 1640 |
Liver: Porcine | |
| |
| Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures
, Am J Physiol Regul Integr Comp Physiol
286,
R206-16, 2004 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | DMEM |
Liver: Porcine | |
| |
| Zhou X, Liu L, Kano J, Mukaiyama T, and Tokiwa T.: Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System
, Chin Med J 114,
946, 2001 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% Neutral Protease: |
| Medium | MEM |
Liver: Porcine | |
| |
| Gerlach, J., Brombacher, J., Smith, M., Neuhaus, P.: High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight
, J Surg Res 62 (1),
85, 1996 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.8% |
| Medium | PBS |
Liver: Rabbit | |
| |
| Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies
, In Vitro 17,
935, 1981 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 90 u/ml |
| Medium | CF EGTA perfusate |
Liver: Rat | |
| |
| Perepelyuk, M., Terajima, M., Wang, A., Georges, P., Janmey, P., Yamauchi, M. and Wells, R.: Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury.
, Am J Physiol Gastrointest Liver Physiol
304,
G605, 2013 | |
| Cells: | Hepatocytes and sinusoidal endothelial |
| Enzyme(s) | Collagenase Type 4: 0.05% Deoxyribonuclease I: 0.003% |
| Medium | HBSS |
Liver: Rat | |
| |
| Shen, L., Hillebrand, A., Wang, D. and Liu, M.: Isolation and Primary Culture of Rat Hepatic Cells.
, J Vis Exp
64,
e3917, 2012 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 1000 U |
| Medium | William's |
Liver: Rat | |
| |
| Wen, J., Olsen, A., Perepelyuk, M. and Wells, R.: Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion.
, J Vis Exp
64,
e3669, 2012 | |
| Cells: | Portal fibroblasts |
| Enzyme(s) | Collagenase Type 2: 0.3% |
| Medium | DMEM/F-12 |
Liver: Rat | |
| |
| Budick-Harmelin, N., Anavi, S., Madar, Z. and Tirosh, O.: Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes.
, Lipids Health Dis
11,
66, 2012 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | DMEM |
Liver: Rat | |
| |
| Pillai, V., and Mehvar, R.: Inhibition of NADPH-Cytochrome P450 Reductase by Tannic Acid in Rat Liver Microsomes and Primary Hepatocytes: Methodological Artifacts and Application to Ischemia-Reperfusion Injury
, J Pharm Sci 100,
3495, 2011 | |
| Cells: | Hepatocytes |
| Enzyme(s) | HIS kit: per instructions |
| Medium | Waymouth's MB |
Liver: Rat | |
| |
| Gopalakrishnan, S. and Harris, E.: In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion.
, J Vis Exp
57,
e3138, 2011 | |
| Cells: | Hepatocytes and SEC's |
| Enzyme(s) | Collagenase Type 1: 0.1-0.2% |
| Medium | RPMI |
Liver: Rat | |
| |
| Zvibel, I., Atias, D., Phillips, A., Halpern, Z. and Oren, R.: Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho.
, Lab Invest 90,
674, 2010 | |
| Cells: | Hepatic stellate cells |
| Enzyme(s) | Collagenase Type 1: 0.025-0.1% Pronase: 0.025-0.13% |
| Medium | DMEM |
Liver: Rat | |
| |
| Handy, J., Saxena, N., Fu, P., Lin, S., Mells, J., Gupta, N. and Anania, F.: Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3).
, J Cell Biochem 110,
1195, 2010 | |
| Cells: | Hepatic stellate cells |
| Enzyme(s) | Pronase: 0.02% Collagenase: see reference |
| Medium | Medium 199 |
Liver: Rat | |
| |
| Parasrampuria, R. and Mehvar, R.: Dose-Dependent Inhibition of Transporter-Mediated Hepatic Uptake and Biliary Excretion of Methotrexate by Cyclosporine A in an Isolated Perfused Rat Liver Model.
, J Pharm Sci 99,
5060, 2010 | |
| Cells: | Hepatocytes |
| Enzyme(s) | HIS kit: per instructions |
| Medium | Krebs-Henseleit |
Liver: Rat | |
| |
| Chung, C., Shugrue, C., Nagar, A., Doll, J., Cornwell, M., Gattu, A., Kolodecik, T., Pandol, S. and Gorelick, F.: Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator.
, Gastroenterol 136,
331, 2009 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HBSS |
Liver: Rat | |
| |
| Bettinger, C., Kulig, K., Vacanti, J., Langer, R. and Borenstein, J.: Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture.
, Tissue Eng Part A
15,
1321, 2009 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DPBS |
Liver: Rat | |
| |
| Mula, N., Cubero, F., Codesal, J., de Andres, S., Escudero, C., Garcia-Barrutia, S., Millan, I., Arahuetes, R. and Maganto, P.: Survival of Allogeneic Hepatocytes Transplanted into the Thymus.
, Cells Tissues Organs
188,
270, 2008 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.067% |
| Medium | HBSS |
Liver: Rat | |
| |
| Doleh, L. and Romani, A.: Biphasic Effect of Extra-Reticular Mg2+ on Hepatic G6P Transport and Hydrolysis.
, Arch Biochem Biophys
467,
283, 2007 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | HEPES |
Liver: Rat | |
| |
| Smith, M. and Mooney, D.: Hypoxia Leads to Necrotic Hepatocyte Death.
, J Biomed Mater Res A
80,
520, 2007 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | Williams E |
Liver: Rat | |
| |
| Charbonneau, A., Unson, C. and Lavoie, J.: High-Fat Diet-Induced Hepatic Steatosis Reduces Glucagon Receptor Content in Rat Hepatocytes: Potential Interaction with Acute Exercise.
, J Physiol
579,
255, 2007 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | see reference |
Liver: Rat | |
| |
| Li, W., Ralphs, K., Slack, J. and Tosh, D.: Keratinocyte Serum-Free Medium Maintains Long-Term Liver Gene Expression and Function in Cultured Rat Hepatocytes by Preventing the Loss of Liver-Enriched Transcription Factors.
, Int J Biochem Cell Biol
39,
541-54, 2007 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.033% |
| Medium | Williams E |
Liver: Rat | |
| |
| Li, Z., Dranoff, J., Chan, E., Uemura, M., Sevigny, J.and Wells, R.: Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture.
, Hepatol 46,
1246-56, 2007 | |
| Cells: | Portal fibroblasts |
| Enzyme(s) | Collagenase: 0.03% Pronase: 0.033% Hyaluronidase: 0.036% |
| Medium | DMEM/F-12 |
Liver: Rat | |
| |
| Isabel Zvibel, Miri Bronstein, Einav Hubel, Ella Bar-Lev, Zamir Halpern, Ran Oren: Isolation, characterization and culture of Thy1-positive cells from fetal rat livers
, World J Gastroenterol
12,
3841-7, 2006 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.22% |
| Medium | HBSS |
Liver: Rat | |
| |
| Sosef MN, Baust JM, Sugimachi K, Fowler A, Tompkins RG, and Toner M.: Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function
, Ann Surg 241,
125, 2005 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: see references |
| Medium | DMEM |
Liver: Rat | |
| |
| Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice
, Hepatol 42,
140-8, 2005 | |
| Cells: | Liver sinusodial endothelial |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Liver: Rat | |
| |
| Putz G, Schmider W, Nitschke R, Kurz G, Blum HE: Synthesis of phospholipid-conjugated bile salts and interaction of bile salt-coated liposomes with cultured hepatocytes
, J Lipid Res 46,
2325-38, 2005 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | Williams E |
Liver: Rat | |
| |
| Jensen C., Jauho E., Santoni-Rugiu E., Holmskov U., Teisner B., Tygstrup N., and Bisgaard H.: Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1
, Am J Pathol 164(4),
1347, 2004 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% Pronase: 1% Deoxyribonuclease I: 0.007% |
| Medium | Williams E |
Liver: Rat | |
| |
| Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich
, World J Gastroenterol
10,
699, 2004 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | RPMI 1640 |
Liver: Rat | |
| |
| Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures
, Am J Physiol Regul Integr Comp Physiol
286,
R206-16, 2004 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | DMEM |
Liver: Rat | |
| |
| Annaert, P., Turncliff, R., Booth, C., Thakker, D. and Brouwer, K.: P-Glycoprotein-Mediated In Vitro Biliary Excretion in Sandwich-Cultured Rat Hepatocytes.
, Drug Metab Dispos 29,
1277-83, 2001 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 200 u/ml |
| Medium | DMEM |
Liver: Rat | |
| |
| Burczynski, M., McMillian, M., Parker, J., Bryant, S., Leone, A., Grant, E., Thorne, J., Zhong, Z., Zivin, R. and Johnson, M.: Cytochrome P450 Induction in Rat Hepatocytes Assessed by Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction and the RNA Invasive Cleavage Assay.
, Drug Metab Dispos 29,
1243, 2001 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.025% |
| Medium | Williams E |
Liver: Rat | |
| |
| Kuddus, R., Nalesnik, M., Subbotin, V., Rao, A., and Gandhi, C.: Enhanced Sythesis and Reduced Metabolism of Endothelin-1 (ET-1) by Hepatocytes - An Important Mechanism of Increased Endogenous Levels of ET-1 in Liver Cirrhosis
, J Hepatol
33,
725, 2000 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Liver: Rat | |
| |
| Low-Baselli A., Hufnagl K., Parzefall W., Schulte-Hermann R., and Grasl-Kraupp B.: Initiated Rat Hepatocytes in Primary Culture: A Novel Tool to Study Alterations in Growth Control During the First Stage of Carcinogenesis
, Carcinogenesis
21,
79, 2000 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.02% |
| Medium | Williams E |
Liver: Rat | |
| |
| Gabriel, A., Kuddus, R., Rao, A., and Gandhi, C.: Down-Regulation of Endothelin Receptors by Transforming Growth Factor B1 in Hepatic Stellate Cells
, J Hepatol
30,
440, 1999 | |
| Cells: | Stellate |
| Enzyme(s) | Protease: 0.02% |
| Medium | HBSS |
Liver: Rat | |
| |
| Wolz E, Liechti H, Notter B, Oesterhelt G, Kistler A: Characterization of metabolites of astaxanthin in primary cultures of rat hepatocytes
, Drug Metab Dispos 27,
456-62, 1999 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 120 u/ml |
| Medium | HBSS |
Liver: Rat | |
| |
| Matsuura T, Gad MZ, Harrison EH, and Ross AC: Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocyte and nonparenchymal cell fractions of rat liver
, J Nutr 127,
218, 1997 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 60 u/ml |
| Medium | HEPES |
Liver: Rat | |
| |
| Alston-Smith, J and Pertoft, H: Isolation of Liver Cells: a System for Obtaining Pure Cells in Monolayer Cultures from a Single Rat Liver,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12B:14.1, 1995 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.04% - 0.06% |
| Medium | PBS |
Liver: Rat | |
| |
| Matsumoto, K and Nakamura, T: Techniques for the Isolation and Cultivation of Hepatocytes using Collagenase,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12B:16.1, 1995 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | DMEM |
Liver: Rat | |
| |
| Rana, B., Mischoulon, D., Xie, Y., Bucher, N., and Farmer, S.: Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors
, Mol Cell Biol
14 (9),
5858, 1994 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Medium 199 |
Liver: Rat | |
| |
| Liu, J., McKim, J., Liu, Y., and Klaassen, C.: Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures
, In Vitro Cell Dev Biol 28,
320, 1992 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | Serum-free medium |
Liver: Rat | |
| |
| Dixit, V., Arthur, M., and Gitnick, G.: Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats
, Cell Transplant 1,
275, 1992 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 200 u/ml |
| Medium | RPMI 1640 |
Liver: Rat | |
| |
| Blouin, M., and Marceau, N.: Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells, J Tiss Cul Meth 13,
117, 1991 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 0.05% Neutral Protease: 0.48% Hyaluronidase: 0.075% |
| Medium | Eagles's MEM |
Liver: Rat | |
| |
| Davila, J., Reddy, C., Davis, P. and Acosta, D.: Toxicity Assessment of Papaverine Hydrochloride and Papaverine-Derived Metabolites in Primary Cultures of Rat Hepatocytes
, In Vitro Cell Dev Biol 26,
515, 1990 | |
| Cells: | Parenchymal hepatocytes |
| Enzyme(s) | Collagenase Type 4: 80 u/ml |
| Medium | HBSS |
Liver: Rat | |
| |
| Liu, J., Kershaw, W., and Klaassen, C.: Rat Primary Hepatocyte Cultures are a Good Model for Examining Metallothionein-Induced Tolerance to Cadmium Toxicity
, In Vitro Cell Dev Biol 26,
75, 1990 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | HBSS, CMF |
Liver: Rat | |
| |
| Kindberg, G., Gudmundsen, O., and Berg, T.: The Effect of Vandate on Receptor-mediated Endocytosis of Asialoorosomucoid in Rat Liver Parenchymal Cells
, J Biol Chem 265,
8999, 1990 | |
| Cells: | Parenchymal |
| Enzyme(s) | Collagenase Type 1: |
| Medium | HEPES, modified |
Liver: Rat | |
| |
| Reddy, S., Amick, G., Cooper, R., and Damun, Z.: Insulin Stimulates the Activity of a Protamine in Isolated Rat Hepatocytes
, J Biol Chem 265,
7748, 1990 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: |
| Medium | Kreb's Ringer bicarbonate buffer |
Liver: Rat | |
| |
| Davila, J., Lenherr, A., and Acosta, D.: Protective Effect of Flavonoids on Drug-Induced Hepatotoxicity In Vitro
, Toxicology 57,
267, 1989 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | Hanks' BSS, CF |
Liver: Rat | |
| |
| Chan, K., Kost, D., and Michalopoulos, G.: Multiple Sequential Periods of DNA Synthesis and Quiescence in Primary Hepatocyte Cultures Maintained on the DMSO-EGF On/Off Protocol
, J Cell Physiol 141,
584, 1989 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.5% |
| Medium | RPMI 1640 |
Liver: Rat | |
| |
| Nagy, I., Ohno-Iwashita, Y., Ohta, M., Nagy, V., Kitani, K., Ando, S., and Imahori, K.: Effect of Perfringolysin O on the Lateral Diffusion Constant of Membrane Proteins of Hepatocytes as Revealed by Fluorescence Recovery After Photobleaching
, Biochim Biophys Acta 939,
551, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Ringer's biocarbonate buffer |
Liver: Rat | |
| |
| Voss, A. and Sprecher, H.: Metabolism of 6,9,12-Octadecatetraenoic and 6,9,12,15-Octadecatetraenoic Acid
, Biochim Biophys Acta 958,
153, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HEPES |
Liver: Rat | |
| |
| Kuiper, J., Zijlstra, F., Kamps, J, and vanBerkel, T.: Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells
, Biochim Biophys Acta 959,
143, 1988 | |
| Cells: | Parenchymal Endothelial Kupffer |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | Krebs Henseleit |
Liver: Rat | |
| |
| Cai, H., He, Z., and Ding, Y.: Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors
, Biochim Biophys Acta 958,
334, 1988 | |
| Cells: | Parenchymal Kupffer |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | HBSS with CaCl2 |
Liver: Rat | |
| |
| Cotariu, D., Barr-Nea, L., Papo, N., and Zaidman, J.: Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes
, Enzyme 40,
212, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HBSS |
Liver: Rat | |
| |
| Braun, L., Mead, J., Panzica, M., Mikumo, R., Bell, G., and Fausto, N.: Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation
, Proc Natl Acad Sci U S A 85,
1539, 1988 | |
| Cells: | Hepatocytes and Nonparenchymal |
| Enzyme(s) | Pronase: 0.1% |
| Medium | HEPES buffer with calcium |
Liver: Rat | |
| |
| Chang, T., and Chang, C.: Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution
, Biochim Biophys Acta 942,
57, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 166 u/ml |
| Medium | DMEM |
Liver: Rat | |
| |
| Gupta, C., Hattori, A., Betschart, J., Virji, M., and Shinozuka, H.: Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet
, Cancer Res 48,
1162, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | MEM |
Liver: Rat | |
| |
| Mathis, G., Walls, S., and Sirica, A.: Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes
, Cancer Res 48,
6145, 1988 | |
| Cells: | Bile ductular epithelial |
| Enzyme(s) | Collagenase Type 1: 220 u/ml |
| Medium | L-15 |
Liver: Rat | |
| |
| McAbee DD and Weigel PH: ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes
, Biochemistry
27,
2061, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | HEPES |
Liver: Rat | |
| |
| Rodriguez de Turco, E., and Spitzer, J.: Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats
, Biochem J
253,
73, 1988 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: |
| Medium | HBSS |
Liver: Rat | |
| |
| Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan
, Anal Biochem
161,
207, 1987 | |
| Cells: | Lipocytes Kupffer Sinusoidal endothelial |
| Enzyme(s) | Collagenase: 0.015% |
| Medium | DMEM/Ham's F-12 |
Liver: Rat | |
| |
| Oka, J. and Weigel, P.: Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes
, J Cell Physiol 133,
243, 1987 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 100 - 200 µg/g body weight |
| Medium | Eagle's Eagle's w/HEPES HBSS |
Liver: Rat | |
| |
| Francavilla, A., Ove, P., Polimeno, L., Sciascia, C., Coetzee, M., and Starzi, T.: Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy
, Cancer Res 46,
1318, 1986 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 125 - 250 u/ml |
| Medium | MEM (see reference) |
Liver: Rat | |
| |
| Kreamer, B., Staecker, J., Sawada, N., Sattler, G., Hsia, M., and Pitot, H.: Use of Low-Speed, Iso-Density Percoll Centrifugation Method to Increase the Viability of Isolated Rat Hepatocyte Preparations
, In Vitro Cell Dev Biol 22 (4),
201, 1986 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HBSS |
Liver: Rat | |
| |
| Schwarz, K., Lanier, S., Carter, E., Homcy, C., and Graham, R.: Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture
, Mol Pharmacol 27,
200, 1985 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Krebs Ringer bicarbonate buffer |
Liver: Rat | |
| |
| Holstege A, Leser HG, Pausch J, Gerok W: Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes
, Eur J Biochem
149,
169-73, 1985 | |
| Cells: | Hepatocytes, Kupffer, endothelial |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Gey's BSS |
Liver: Rat | |
| |
| Brass, E., Garrity, M., and Robertson, R.: Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins
, F.E.B.S. Lett. 169,
293, 1984 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.04% |
| Medium | Bicarbonate buffer with calcium added |
Liver: Rat | |
| |
| Okumura, T. and Saito, K.: Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes
, J. Biochem.
96,
429, 1984 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Trypsin: 0.005% |
| Medium | Williams E |
Liver: Rat | |
| |
| Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats
, In Vitro 19,
576, 1983 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HBSS CMF |
Liver: Rat | |
| |
| Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells
, J Biol Chem 258,
12221, 1983 | |
| Cells: | Endothelial Kupffer Parenchymal |
| Enzyme(s) | Collagenase: 0.05% Pronase: 0.25% |
| Medium | HBSS |
Liver: Rat | |
| |
| Studer, R. and Borle, A.: Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines
, J Biol Chem 257,
7987, 1982 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 100 u/ml |
| Medium | Krebs Henseleit bicarbonate buffer |
Liver: Rat | |
| |
| Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels
, Exp Cell Res
131,
363, 1981 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HBSS, CMF |
Liver: Rat | |
| |
| Goldstein, A., Palmer, J., and Johnson, P.: Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis
, In Vitro 17,
651, 1981 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 2: 0.30% |
| Medium | Dulbecco-Vogt arginine free Eagle's |
Liver: Rat | |
| |
| Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies
, In Vitro 17,
935, 1981 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 90 u/ml |
| Medium | CF EGTA perfusate |
Liver: Rat | |
| |
| Gugen-Guillouzo, C., Tichonicky, L., Szajnert, M., and Kruh, J.: Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes
, In Vitro 16,
1, 1980 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.025% |
| Medium | HEPES buffer |
Liver: Rat | |
| |
| Yamada, S., Otto, P., Kennedy, D., and Whayne, T.: The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture
, In Vitro 16,
559, 1980 | |
| Cells: | Parenchymal |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | Kreb's Henseleit bicarbonate buffer |
Liver: Rat | |
| |
| De Gerlache, J., Lans, M., Taper, H., and Roberfroid, M.: Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization
, Toxicology 18,
225, 1980 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Hyaluronidase: 0.02% |
| Medium | Kreb's buffer |
Liver: Rat | |
| |
| Acosta, D., Anuforo, D., and Smith, R: Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells, J Tiss Cul Meth 6,
35, 1980 | |
| Cells: | Liver |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HBSS modified (see reference) |
Liver: Rat | |
| |
| Poli, G., Gravela, E., Albano, E., and Dianzani, M.: Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion
, Exp Mol Pathol 30,
116, 1979 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Hyaluronidase: 460 u/ml |
| Medium | Saline |
Liver: Rat | |
| |
| Davis, R., Engelhorn, S., Pangburn, S., Weinstein, D., and Steinberg, D.: Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes
, J Biol Chem 254 (6),
2010, 1979 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.065% |
| Medium | DMEM (see reference) |
Liver: Rat | |
| |
| Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars
, J Biol Chem 254 (21),
10830, 1979 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 4: 6000 units Collagenase Type 3 & 4: |
| Medium | HEPES |
Liver: Rat | |
| |
| Rubin, K., Kjellen, L., and Oslashbrink, B.: Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel
, Exp Cell Res
109,
413, 1977 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 100 u/ml |
| Medium | Buffers 1 & 2 (see reference) |
Liver: Rat | |
| |
| VanBezodijen, C., Grell, T., and Knook, D.: Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells
, Mech Ageing Dev 6,
293, 1977 | |
| Cells: | Parenchymal |
| Enzyme(s) | Collagenase Type 1: 0.05% - 0.06% |
| Medium | HEPES buffer |
Liver: Rat | |
| |
| Williams, G., Bermudez, E., and Scaramuzzino, D.: Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium
, In Vitro 13 (12),
809, 1977 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 100 u/ml |
| Medium | HBSS (see reference) |
Liver: Rat | |
| |
| Gravela, E., Poli, G., Albano, E., and Dianzani, M.: Studies of Fatty Liver with Isolated Hepatocytes
, Exp Mol Pathol 27,
339, 1977 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Hank's solution, CF |
Liver: Rat | |
| |
| Laishes, B., and Williams, G.: Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin
, In Vitro 12,
521, 1976 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.05%-0.10% |
| Medium | Williams E |
Liver: Rat | |
| |
| Witters, L., Alberico, L., and Acruch, J.: Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte
, Biochem Biophys Res Commun
69 (4),
997, 1976 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | MEM |
Liver: Rat | |
| |
| Bausher, J., and Schaeffer, W.: A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1
, In Vitro 9,
286, 1974 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS, CMF |
Liver: Rat | |
| |
| Bonney, R., Becker, J., Walker, P., and Potter, V.: Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis
, In Vitro 9,
399, 1974 | |
| Cells: | Parenchymal |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Hank's solution, CMF |
Liver: Rat | |
| |
| Gerschenson, L., Berliner, J., and Davidson, M.: The Isolation and Culture of Liver Cells
, Vol. 32,, ,
733, 1974 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Hyaluronidase: 0.10% |
| Medium | HBSS |
Liver: Rat | |
| |
| Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells
, Exp Cell Res
89,
139, 1974 | |
| Cells: | Epithelial |
| Enzyme(s) | Hyaluronidase: 0.0075% |
| Medium | KCl-NaCl HEPES Buffer |
Liver: Rat | |
| |
| Howard, R., Lee, J., and Pesch, L.: The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques
, J Cell Biol
57,
642, 1973 | |
| Cells: | Parenchymal |
| Enzyme(s) | Hyaluronidase: 0.10% |
| Medium | HBSS, CF |
Liver: Rat | |
| |
| Bissell, D., Hammaker, L., and Meyer, U.: Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies
, J Cell Biol
59,
722, 1973 | |
| Cells: | Parenchymal |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | Hank's solution, CF |
Liver: Rat | |
| |
| Berg, T., Boman, D., and Seglen, P.O.: Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone
, Exp Cell Res
72,
571, 1972 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Hyaluronidase: 0.10% |
| Medium | HBSS, CF |
Liver: Rat | |
| |
| Johnson, M., Das, N., Butcher, F., and Fain, J.: The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids
, J Biol Chem 247,
3229, 1972 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Hyaluronidase: 0.08% |
| Medium | HBSS, CF |
Liver: Rat | |
| |
| Leffert, H., and Paul, D.: Studies on Primary Cultures of Differentiated Fetal Liver Cells
, J Cell Biol
52,
559, 1972 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | Modified Eagle's w/ Serum |
Liver: Rat | |
| |
| Seglen, P.: Preparation of Rat Liver Cells
, Exp Cell Res
74,
450, 1972 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Collagenase: 0.01 - 0.08% |
| Medium | HEPES |
Liver: Rat | |
| |
| Iype, P.: Cultures from Adult Rat Liver Cells. 1.Establishment of Monolayer Cell-Cultures from Normal Lver
, J Cell Physiol 78,
281, 1971 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Hyaluronidase: 1.0% |
| Medium | HBSS, CMF |
Liver: Rat | |
| |
| Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver
, Exp Cell Res
69,
106, 1971 | |
| Cells: | Epithelial-like |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS |
Liver: Rat | |
| |
| Haung, Y., and Ebner, K.: Induction of Tyrosine Aminotransferase in Isolated Liver Cells
, Biochim Biophys Acta 191,
161, 1969 | |
| Cells: | Hepatocytes |
| Enzyme(s) | Hyaluronidase: 0.10% |
| Medium | HBSS, CF |
Liver: Rat | |
| |
| Berry, M., and Friend, D.: High Yield Preparation of Isolated Rat Liver Parenchymal Cells
, J Cell Biol
43,
506, 1969 | |
| Cells: | Parenchymal |
| Enzyme(s) | Hyaluronidase: 0.10% Collagenase Type 1: 0.05% |
| Medium | HBSS, CF |
Lung: Bovine | |
| |
| Del Vecchio, P., Siflinger-Birnboim, A., Belloni, P., Holleran, L., Lum, H., and Malik, A.: Culture and Characterization of Pulmonary Microvascular Endothelial Cells
, In Vitro Cell Dev Biol 28A,
711, 1992 | |
| Cells: | Endothelial Pulmonary |
| Enzyme(s) | Collagenase: 1000 u/ml Collagenase Type 2: 1000 u/ml |
| Medium | PBS, CMF |
Lung: Guinea-Pig | |
| |
| Sikpi, M., Nair, C., Johns, A., and Das, S.: Metabolic and Ultrastructural Characterizationof Guinea-Pig Alveolar Type II Cells Isolated by Centrifugal Elutriation
, Biochim Biophys Acta 877,
20, 1986 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Elastase: 40 u/ml |
| Medium | PBS (see reference) |
Lung: Guinea-Pig | |
| |
| Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase
, In Vitro 8,
101, 1972 | |
| Cells: | Single cells |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | CF salt solution |
Lung: Human | |
| |
| Bahmed, K., Boukhenouna, S., Karim, L., Andrews, T., Lin, J., Powers, R., Wilson, M., Lin, C., Messier, E., Reisdorph, N., Powell, R., Tang, H., Mason, R., Criner, G. and Kosmider, B.: The Effect of Cysteine Oxidation on DJ-1 Cytoprotective Function in Human Alveolar Type II Cells.
, Cell Death Dis
10,
638, 2019 | |
| Cells: | Aveolar |
| Enzyme(s) | Elastase: 12.9 u/ml |
| Medium | PBS |
Lung: Human | |
| |
| Quatromoni, J., Singhal, S., Bhojnagarwala, P., Hancock, W., Albelda, S. and Eruslanov, E.: An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells
, J Leukoc Biol 97,
201, 2015 | |
| Cells: | Lung tumor |
| Enzyme(s) | Collagenase Type 1: 45-60 u/ml Collagenase Type 2: 15-20 u/ml Collagenase Type 4: 45-60 u/ml Elastase: 0.002% Deoxyribonuclease I: 0.002% |
| Medium | DMEM/F12 |
Lung: Human | |
| |
| Barkauskas, C., Cronce, M., Rackley, C., Bowie, E., Keene, D., Stripp, B., Randell, S., Noble, P. and Hogan, B.: Type 2 Alveolar Cells are Stem Cells in Adult Lung
, J Clin Invest 123,
3025, 2013 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Neutral Protease: 2 u/ml Trypsin: 0.25% Elastase: 10 u/ml |
| Medium | Bronchial Epithelial Growth Medium |
Lung: Human | |
| |
| Mackay, L., Dodd, S., Dougall, I., Tomlinson, W., Lordan, J., Fisher, A. and Corris, P.: Isolation and Characterisation of Human Pulmonary Microvascular Endothelial Cells from Patients with Severe Emphysema.
, Respir Res 14,
23, 2013 | |
| Cells: | Pulmonary endothelial |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | RPMI |
Lung: Human | |
| |
| Comhair, S., Xu, W., Mavrakis, L., Aldred, M., Asosingh, K. and Erzurum, S.: Human Primary Lung Endothelial Cells in Culture.
, Am J Respir Cell Mol Biol 46,
723-30, 2012 | |
| Cells: | Lung endothelial |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | PBS |
Lung: Human | |
| |
| Fujino, N., Kubo, H., Suzuki, T., Ota, C., Hegab, A., He, M., Suzuki, S., Suzuki, T., Yamada, M., Kondo, T., Kato, H. and Yamaya, M.: Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs.
, Lab Invest 91,
363, 2011 | |
| Cells: | Lung |
| Enzyme(s) | Neutral Protease: 2 u/ml Collagenase/Dispase: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Lung: Human | |
| |
| Zhu, Y., Skold, C., Liu, X., Wang, H., Kohyama., T., Wen, F., Ertl, R., and Rennard, S.: Fibroblasts and Monocyte Macrophages Contract and Degrade Three-Dimensional Collagen Gels in Extended Co-Culture
, Respir Res 2 (5),
295, 2001 | |
| Cells: | Lung Fibroblasts |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Lung: Human | |
| |
| Liley, H., Ertsey, R., Gonzales, L., Odom, M., Hawgood, S., Dobbs, L., and Ballard, P.: Synthesis of Surfactant Components by Cultured Type II Cells From Human Lung
, Biochim Biophys Acta 961,
86, 1988 | |
| Cells: | Alveolar type II Fetal |
| Enzyme(s) | Trypsin: 50 ug/ml |
| Medium | Ham's F-12, Eagle's MEM |
Lung: Human | |
| |
| Lechner, J., Haugen, A., McClendon, I., and Pettis, E.: Clonal Growth of Normal Adult Human Bronchial Epithelial Cells in a Serum-Free Medium
, In Vitro 18 (7),
633, 1982 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.02% |
| Medium | Medium 199 |
Lung: Human | |
| |
| Kan, M., and Yamane, I.: In Vitro Proliferation and Lifespan of Human Diploid Fibroblasts in Serum-Free BSA-Containing Medium
, J Cell Physiol 111,
155, 1982 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Trypsin: 0.01% |
| Medium | Eagle's MEM |
Lung: Human | |
| |
| Hinz, R., and Syverton, J.: Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase
, Proc Soc Exp Biol Med 101,
19, 1959 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase: 0.01% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Qiu, Y., Xu, Y., Wang, M., Miao, M., Zhou, H., Xu, J., Kong, J., Zheng, D., Li, R., Zhang, R., Guo, Y., Li, X., Cui, J., Qin, C. and Zhou , X.: Flavivirus Induces and Antagonizes Antiviral RNA Interference in Both Mammals and Mosquitoes.
, Sci. Adv.
6,
7989, 2020 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Collagenase Type 2: 1.0% Deoxyribonuclease I: 0.001% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Wang, J., Niu, N., Xu, S. and Jin, Z.: A Simple Protocol for Isolating Mouse Lung Endothelial Cells.
, Sci Rep
9,
1458, 2019 | |
| Cells: | Lung endothelial |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Lung: Mouse | |
| |
| Lewis, K., Hall, J., Kiyotake, E., Christensen, T., Balasubramaniam, V. and Anseth, K.: Epithelial-Mesenchymal Crosstalk Influences Cellular Behavior in a 3D Alveolus-Fibroblast Model System.
, Biomaterials 155,
124, 2018 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Collagenase Type 1: 0.1% Deoxyribonuclease I: 0.1% Trypsin: 0.01% |
| Medium | DMEM/F-12 |
Lung: Mouse | |
| |
| Sun, F., Xiao, G. and Qu, Z.: Isolation of Murine Alveolar Type II Epithelial Cells.
, Bio-protocol
7,
, 2017 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Neutral Protease: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Lung: Mouse | |
| |
| Yu, Y., O'Koren, E., Holten, D., Kan, M., Kojin, D., Nelson, E., Que, L. and Gunn, M.: A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflammed Murine Non-Lymphoid Tissues
, PLoS ONE
10,
1371, 2016 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 1: 0.15% Deoxyribonuclease I: 0.04% |
| Medium | PBS |
Lung: Mouse | |
| |
| Braza, F., Dirou, S., Forest, V., Sauzeau, V., Hassoun, D., Chesne, J., Cheminant-Muller, M., Sagan, C., Magnan, A. and Lemarchand, P.: Mesenchymal Stem Cells Induce Suppressive Macrophages Through Phagocytosis in a Mouse Model of Asthma.
, Stem Cells 34,
1836-45, 2016 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 2: 0.3% Deoxyribonuclease I: 0.3% |
| Medium | PBS |
Lung: Mouse | |
| |
| Singer, B., Mock, J., D'Alessio, F., Aggarwal, N., Mandke, P., Johnston, L. and Damarla, M.: Flow-Cytometric Method for Simultaneous Analysis of Mouse Lung Epithelial, Endothelial, and Hematopoietic Lineage Cells.
, Am J Physiol Lung Cell Mol Physiol
310,
L796-801, 2016 | |
| Cells: | Epithelial, Endothelial |
| Enzyme(s) | Collagenase Type 1: 0.5% Deoxyribonuclease I: 0.1% Neutral Protease: 0.1% |
| Medium | PBS |
Lung: Mouse | |
| |
| Mohapatra, A., Van Dyken, S., Schneider, C., Nussbaum, J., Liang, H. and Locksley, R.: Group 2 Innate Lymphoid Cells Utilize the IRF4-IL-9 Module to Coordinate Epithelial Cell Maintenance of Lung Homeostasis.
, Mucosal Immunol
,
, 2015 | |
| Cells: | Alveolar |
| Enzyme(s) | Collagenase Type 1: 300 u/ml Elastase: 4 u/ml Neutral Protease: 5 u/ml Deoxyribonuclease I: 200 u/ml |
| Medium | HBSS |
Lung: Mouse | |
| |
| Kim, H., Lee, H., Chang, Y., Pichavant, M., Shore, S., Fitzgerald, K., Ikawura, Y., Israel, E., Bolger, K., Faul, J., DeKruyff, R. and Umetsu, D.: IL-17 Producing Innate Lymphoid Cells and the NLRP3 Inflammasone Facilitate Obescity-associated Airway Hyperractivity
, Nat Med 20,
54, 2014 | |
| Cells: | Pulmonary |
| Enzyme(s) | Collagenase Type 4: 0.16% Deoxyribonuclease I: 0.1% |
| Medium | see reference |
Lung: Mouse | |
| |
| Desai, T., Brownfield, D. and Krasnow, M.: Alveolar Progenitor and Stem Cells in Lung Development, Renewal and Cancer.
, Nature
507,
190-4, 2014 | |
| Cells: | Avelolar progenitor |
| Enzyme(s) | Elastase: 3 u/ml Deoxyribonuclease I: 0.33 u/ml |
| Medium | DMEM/F12 |
Lung: Mouse | |
| |
| Guimond, D., Cam, N., Hirve, N., Duan, W., Lambris, J., Croft, M. and Tsoukas, C.: Regulation of Immune Responsiveness In Vivo by Disrupting an Early T-Cell Signaling Event Using a Cell-Permeable Peptide.
, PLoS ONE
8,
e63645, 2013 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 4: 0.3% |
| Medium | PBS |
Lung: Mouse | |
| |
| Misharin, A., Morales-Nebreda, L., Mutlu, G., Budinger, G. and Perlman, H.: Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung.
, Am J Respir Cell Mol Biol 49,
503-10, 2013 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Driscoll, B., Kikuchi, A., Lau, A., Lee, J., Reddy, R., Jesudason, E., Kim, C. and Warburton, D.: Isolation and Characterization of Distal Lung Progenitor Cells.
, Methods Mol Biol 879,
109, 2012 | |
| Cells: | Lung progenitor |
| Enzyme(s) | Collagenase/Dispase: 0.2% Neutral Protease: 5 u/ml Deoxyribonuclease I: 0.0025% |
| Medium | DMEM |
Lung: Mouse | |
| |
| Vaughan, A., Halbert, C., Wootton, S. and Miller, A.: Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation.
, Mol Cancer Res
10,
86, 2012 | |
| Cells: | Lung tumor |
| Enzyme(s) | Neutral Protease: 50 u/ml Collagenase: 400 u/ml Deoxyribonuclease I: 50 u/ml |
| Medium | DMEM |
Lung: Mouse | |
| |
| Hardy, C., LeMasurier, J., Belz, G., Scalzo-Inguanti, K., Yao, J., Xiang, S., Kanellakis, P., Bobik, A., Strickland, D., Rolland, J., O'Hehir, R. and Plebanski, M.: Inert 50-nm Polystyrene Nanoparticles That Modify Pulmonary Dendritic Cell Function and Inhibit Allergic Airway Inflammation.
, J Immunol 188,
1431, 2012 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 3: 0.1% Deoxyribonuclease I: 0.0025% |
| Medium | RPMI 1640 |
Lung: Mouse | |
| |
| Rayamajhi, M., Redente, E., Condon, T., Gonzalez-Juarrero, M., Riches, D. and Lenz, L.: Non-Surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry.
, J Vis Exp
51,
2702, 2011 | |
| Cells: | Lung draining |
| Enzyme(s) | Collagenase Type 4: 0.1-0.125% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Chow, K., Jun, D., Helm, K., Wagner, D. and Majka, S.: Isolation & Characterization of Hoechst(low) CD45(negative) Mouse Lung Mesenchymal Stem Cells.
, J Vis Exp
56,
e3159, 2011 | |
| Cells: | Lung mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Lancelin, W. and Guerrero-Plata, A.: Isolation of Mouse Lung Dendritic Cells.
, J Vis Exp
57,
e3563, 2011 | |
| Cells: | Dendritic |
| Enzyme(s) | Collagenase Type 1: 0.05% Deoxyribonuclease I: 0.002% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Rock, J, Barkauskas, C, Cronc, M, Xue, Y, Harris, J, Liang, J, Noble, P and Hogan, B.: Multiple Stromal Populations Contribute to Pulmonary Fibrosis Without Evidence for Epithelial to Mesenchymal Transition.
, Proc Natl Acad Sci U S A 108,
E1475, 2011 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 1: 450 u/ml Elastase: 4 u/ml Neutral Protease: 5 u/ml Deoxyribonuclease I: 0.33 u/ml |
| Medium | DMEM/F12 |
Lung: Mouse | |
| |
| Breslow, R., Rao, J., Xing, W., Hong, D., Barrett, N. and Katz, H.: Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells.
, J Immunol 184,
1003, 2010 | |
| Cells: | Lung mononuclear |
| Enzyme(s) | Collagenase Type 4: 500 u/ml Deoxyribonuclease I: 0.002% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Ferreira, C., Antunes, F., Leonard, V., Welstead, G., Richardson, C. and Cattaneo, R.: Measles Virus Infection of Alveolar Macrophages and Dendritic Cells Precedes Spread to Lymphatic Organs in Transgenic Mice Expressing Human Signaling Lymphocytic Activation Molecule (SLAM, CD150).
, J Virol 84,
3033, 2010 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 2: 300 u/ml Deoxyribonuclease I: 0.015% |
| Medium | RPMI 1640 |
Lung: Mouse | |
| |
| Trotter, A., Kipp, M., Schrader, R. and Beyer, C.: Combined Application of 17Beta-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice.
, Int J Pediatr
2009,
170491, 2009 | |
| Cells: | Alveolar and fibroblast |
| Enzyme(s) | Trypsin: 2.5% Deoxyribonuclease I: 0.2% Collagenase Type 1: 1250 u/ml |
| Medium | MEM |
Lung: Mouse | |
| |
| Zhao, J., Zhao, J., Van Rooijen, N. and Perlman, S.: Evasion by Stealth: Inefficient Immune Activation Underlies Poor T Cell Response and Severe Disease in SARS-CoV-Infected Mice.
, PLoS Pathog 5,
e1000636, 2009 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Flano, E., Jewell, N., Durbin, R. and Durbin, J.: Methods Used to Study Respiratory Virus Infection.
,Curr Protoc Cell Biol Vol. Chapter 26,, ,
Unit 26.3, 2009 | |
| Cells: | Dendritic |
| Enzyme(s) | Collagenase Type 1: 0.5% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Jungblut, M., Oeltze, K., Zehnter, I., Hasselmann, D. and Bosio, A.: Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue Using the GentleMACS Dissociator.
, J Vis Exp
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| Cells: | Leukocytes and endothelial |
| Enzyme(s) | Collagenase: 0.2% Deoxyribonuclease I: 40-80 u/ml |
| Medium | PBS |
Lung: Mouse | |
| |
| Jones, R., Capen, D., Cohen, K., Munn, L., Jain, R. and Duda, D.: A Protocol for Phenotypic Detection and Characterization of Vascular Cells of Different Origins in a Lung Neovascularization Model in Rodents.
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3,
388-97, 2008 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | PBS |
Lung: Mouse | |
| |
| Ebeling, C., Lam, T., Gordon, J., Hollenberg, M. and Vliagoftis, H.: Proteinase-Activated Receptor-2 Promotes Allergic Sensitization to an Inhaled Antigen through a TNF-Mediated Pathway.
, J Immunol 179,
2910, 2007 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 3: 0.17% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Finotto, S., Eigenbrod, T., Karwot, R., Boross, I., Doganci, A., Ito, H., Nishimoto, N., Yoshizaki, K., Kishimoto, T., Rose-John, S., Galle, P. and Neurath, M.: Local Blockade of IL-6R Signaling Induces Lung CD4+ T Cell Apoptosis in a Murine Model of Asthma Via Regulatory T Cells.
, Int Immunol 19,
685, 2007 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 2: 300 u/ml Deoxyribonuclease I: 0.001% |
| Medium | Dulbecco's PBS |
Lung: Mouse | |
| |
| Abonia, J., Hallgren, J., Jones, T., Shi, T., Xu, Y., Koni, P., Flavell, R., Boyce, J., Austen, F. and Gurish, M.: Alpha-4 Integrins and VCAM-1, but not MAdCAM-1, are Essential for Recruitment of Mast Cell Progenitors to the Inflamed Lung.
, Blood 108,
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| Cells: | Mononuclear |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | RPMI 1640 |
Lung: Mouse | |
| |
| Woolard MD, Hodge LM, Jones HP, Schoeb TR, and Simecka JW: The upper and lower respiratory tracts differ in their requirement of IFN-gamma and IL-4 in controlling respiratory mycoplasma infection and disease
, J Immunol 172,
6875, 2004 | |
| Cells: | Mononuclear cells |
| Enzyme(s) | Collagenase Type 1: 300 u/ml Deoxyribonuclease I: 50 u/ml |
| Medium | RPMI 1640 medium |
Lung: Mouse | |
| |
| Andonegui G., Bonder C., Green F., Mullaly S., Zbytnuik L., Raharjo E., and Kubes P.: Endothelium-derived Toll-like Receptor-4 is the Key Molecule in LPS-induced Neutrophil Sequestration into Lungs
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| Cells: | Murine pulmonary endothelial |
| Enzyme(s) | Collagenase Type 2: 0.25% |
| Medium | HBSS |
Lung: Mouse | |
| |
| Huaux F, Liu T, McGarry B, Ullenbruch M, and Phan SH.: Dual roles of IL-4 in lung injury and fibrosis
, J Immunol 170,
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| Cells: | Pulmonary T lymphocytes |
| Enzyme(s) | Collagenase Type 3: 10 mg/lung Deoxyribonuclease I: 250 ug/lung |
| Medium | RPMI medium |
Lung: Mouse | |
| |
| Paine R 3rd, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CE, Matthay MA, and Christensen PJ: Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury
, Am J Pathol 163,
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| Cells: | Alveolar epithelial cells |
| Enzyme(s) | Neutral Protease: Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Lung: Mouse | |
| |
| Vermaelen KY, Carro-Muino I, Lambrecht BN, and Pauwels RA.: Specific migratory dendritic cells rapidly transport antigen from the airways to the thoracic lymph nodes
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51, 2001 | |
| Cells: | Lung and lymph node cells |
| Enzyme(s) | Collagenase Type 2: 0.1% Deoxyribonuclease I: 0.002% |
| Medium | RPMI 1640 |
Lung: Mouse | |
| |
| Freedman SD, Katz MH, Parker EM, Laposata M, Urman MY, and Alvarez JG.: A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice
, Proc Natl Acad Sci U S A 96,
13995, 1999 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase: 100 u/ml Deoxyribonuclease I: 200 u/ml |
| Medium | Krebs-Henseleit Buffer |
Lung: Mouse | |
| |
| Stampfli MR, Wiley RE, Neigh GS, Gajewska BU, Lei XF, Snider DP, Xing Z, Jordana M: GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice
, J Clin Invest 102,
1704-14, 1998 | |
| Cells: | Lung cells |
| Enzyme(s) | Collagenase Type 3: 150 u/ml |
| Medium | HBSS |
Lung: Mouse | |
| |
| Dong QG, Bernasconi S, Lostaglio S, De Calmanovici RW, Martin-Padura I, Breviario F, Garlanda C, Ramponi S, Mantovani A,and Vecchi A.: A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants
, Arterioscler Thromb Vasc Biol
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| Cells: | Murine endothelial cells |
| Enzyme(s) | Collagenase Type 1: 1 mg/ml |
| Medium | DMEM |
Lung: Mouse | |
| |
| Hamilton-Easton A, and Eichelberger M: Virus-specific antigen presentation by different subsets of cells from lung and mediastinal lymph node tissues of influenza virus-infected mice
, J Virol 69,
6359, 1995 | |
| Cells: | Antigen presenting cells |
| Enzyme(s) | Collagenase: 150 u/ml Deoxyribonuclease I: 30 u/ml |
| Medium | RPMI 1640 |
Lung: Porcine | |
| |
| Hinz, R., and Syverton, J.: Mammalian Cell Cultures for Study of Influenza Virus. I. Preparation of Monolayer Cultures with Collagenase
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| Cells: | Lung |
| Enzyme(s) | Collagenase: 0.01% |
| Medium | HBSS |
Lung: Rabbit | |
| |
| Scott, J.: The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung
, Exp Lung Res 12,
181, 1987 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HBSS |
Lung: Rabbit | |
| |
| Finkelstein, J., Maniscalco, W., and Shapiro, D.: Properties of Freshly Isolated Type II Alveolar Epithelial Cells
, Biochim Biophys Acta 762,
398, 1983 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Trypsin: 0.0025% |
| Medium | Joklik's MEM |
Lung: Rabbit | |
| |
| Devereux, T., and Fouts, J.: Isolation and Identification of Clara Cells From Rabbit Lung
, In Vitro 16 (11),
958, 1980 | |
| Cells: | Clara cells |
| Enzyme(s) | Protease: 0.1% |
| Medium | HEPES |
Lung: Rabbit | |
| |
| Gould, M., Clements, J., Jones, A., and Felts, J.: Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism
, Science 178,
1209, 1972 | |
| Cells: | Lung |
| Enzyme(s) | Pronase: 0.2% |
| Medium | Kreb's serum substitute solution, CMF |
Lung: Rat | |
| |
| Jones, R., Capen, D., Cohen, K., Munn, L., Jain, R. and Duda, D.: A Protocol for Phenotypic Detection and Characterization of Vascular Cells of Different Origins in a Lung Neovascularization Model in Rodents.
, Nat Protoc
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388-97, 2008 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | PBS |
Lung: Rat | |
| |
| Chen J., Chen Z., Narasaraju T., Jin N., and Liu L.: Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs
, Lab Invest 84,
727, 2004 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Elastase: 3-4.5 u/ml |
| Medium | RPMI 1640 |
Lung: Rat | |
| |
| King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F, and Stevens T: Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes
, Microvasc Res 67,
139, 2004 | |
| Cells: | Pulmonary endothelial cells |
| Enzyme(s) | Collagenase Type 2: 1000 u/ml |
| Medium | DMEM/F-12 |
Lung: Rat | |
| |
| Liebler, J., Borok, Z., Li, X., Zhou, B., Sandoval, A., Kim, K.and Crandall, E.: Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2.
, J Histochem Cytochem 52,
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| Cells: | Alveolar type I & II |
| Enzyme(s) | Elastase: 2.5-8 u/ml Collagenase Type 1: 1.0% |
| Medium | DMEM/F12 |
Lung: Rat | |
| |
| Sunil VR, Connor AJ, Guo Y, Laskin JD, and Laskin DL: Activation of type II alveolar epithelial cells during acute endotoxemia
, Am J Physiol Lung Cell Mol Physiol
282,
L872, 2002 | |
| Cells: | Type II alveolar epithelial cells |
| Enzyme(s) | Elastase: 4.2 u/ml Deoxyribonuclease I: 0.0001% |
| Medium | DMEM |
Lung: Rat | |
| |
| Kemp, P., Kim, K., Borok., Z., and Crandall, E.: Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels
, J Physiol
536 (3),
693, 2001 | |
| Cells: | Alveolar |
| Enzyme(s) | Elastase: 2.0 - 2.5 u/ml |
| Medium | DMEM/Ham's F-12 (see reference) |
Lung: Rat | |
| |
| Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A: Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages
, Am J Respir Cell Mol Biol 22,
481-90, 2000 | |
| Cells: | Interstitial lung macrophages |
| Enzyme(s) | Collagenase Type 1: 100 u/ml Deoxyribonuclease I: 50 u/ml |
| Medium | RPMI-1640 |
Lung: Rat | |
| |
| Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair
, J Cell Sci
112,
243, 1999 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Elastase: 40 u/ml |
| Medium | DMEM |
Lung: Rat | |
| |
| Bakhramov, A, Evans, M and Kozlowski, R: Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree
, Exp Physiol 83,
337-47, 1998 | |
| Cells: | Pulmonary arterial myocytes |
| Enzyme(s) | Collagenase: 0.15% Papain: 0.15% Elastase: 0.05% |
| Medium | PBS |
Lung: Rat | |
| |
| Fraslon-Vanhulle, C, Bourbon, J and Batenburg, J: Culture of Fetal Alveolar Epithelial Type II Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
13A:2.1, 1995 | |
| Cells: | Fetal alveolar epithelial type II |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | DMEM |
Lung: Rat | |
| |
| Kim, K., Suh, D., Lubman, R., Danto, S., Borok, Z., and Crandall, E.: Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers, J Tiss Cul Meth 14,
187, 1992 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Elastase: 2 u/ml |
| Medium | EBSS |
Lung: Rat | |
| |
| Berk, J., Franzblau, C., and Goldstein, R.: Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype
, J Biol Chem 266,
3192, 1991 | |
| Cells: | Interstitial |
| Enzyme(s) | Trypsin: 1.125% |
| Medium | HEPES buffer |
Lung: Rat | |
| |
| Fraslon, C., Rolland, G., Bourbon, J., Rieutort, M., and Valenza, C.: Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium
, In Vitro Cell Dev Biol 27A,
843, 1991 | |
| Cells: | Alveolar epithelial type II |
| Enzyme(s) | Trypsin: 1% |
| Medium | Eagle's MEM |
Lung: Rat | |
| |
| Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium
, In Vitro Cell Dev Biol 27A,
625, 1991 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS |
Lung: Rat | |
| |
| Mangum, J., Everitt, J., Bonner, J., Moore, L., and Brody, A.: Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins
, In Vitro Cell Dev Biol 26,
1135, 1990 | |
| Cells: | Alveolar type II pneumocytes |
| Enzyme(s) | Elastase: 40 u/ml |
| Medium | HEPES buffer (see reference) |
Lung: Rat | |
| |
| Ma, J., LaCagnin, L., Bowman, L., and Miles, P.: Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells
, Biochim Biophys Acta 1003,
136, 1989 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Elastase: 40 u/ml |
| Medium | Phosphate-buffered medium (see reference) |
Lung: Rat | |
| |
| Batenburg, J., Otto-Verberne, C., Have-Opbroek, A., and Klazinga, W.: Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture
, Biochim Biophys Acta 960,
441, 1988 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | RPMI 1640 |
Lung: Rat | |
| |
| Brown, L. and Longmore, W.: Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats
, Biochim Biophys Acta 878,
258, 1986 | |
| Cells: | Pneumocytes type II |
| Enzyme(s) | Trypsin: 0.30% |
| Medium | BSS |
Lung: Rat | |
| |
| Dobbs, L., Gonzalez R., and Williams, M.: An Improved Method for Isolating Type ll Cells in High Yield and Purity
, Am Rev Respir Dis 134,
141, 1986 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Elastase: 4.3 u/ml |
| Medium | HEPES (see reference) |
Lung: Rat | |
| |
| Weller, N., and Karnovksy, M.: Isolation of Pulmonary Alveolar Type I Cells From Adult Rats
, Am J Pathol 124,
448, 1986 | |
| Cells: | Alveolar type I |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Lung: Rat | |
| |
| Post, M., Torday, J., and Smith, B.: Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor
, Exp Lung Res 7,
53, 1984 | |
| Cells: | Fibroblasts & type II |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HBSS: serum free MEM, CMF |
Lung: Rat | |
| |
| Goodman, B., Fleischer, R., and Crandall, E.: Evidence for Active Na+ Transport by Cultured Monolayers of Pulmonary Alveolar Epithelial Cells
, Am J Physiol 245,
C78, 1983 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Elastase: 4 u/ml |
| Medium | Auto-Pow Eagle's modified MEM |
Lung: Rat | |
| |
| Mason, R., Williams, M., Greenleaf, R., and Clements, J.: Isolation and Properties of Type II Alveolar Cells from Rat Lung
, Am Rev Respir Dis 115,
1015, 1977 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Trypsin: 0.30% |
| Medium | BSS |
Lung: Rat | |
| |
| King, R.: Metabolic Fate of the Apoproteins of Pulmonary Surfactant
, Am Rev Respir Dis 115,
73, 1977 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Trypsin: 0.50% |
| Medium | Earle's MEM |
Lung: Rat | |
| |
| Douglas, W., and Teel, R.: An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes
, Am Rev Respir Dis 113,
17, 1976 | |
| Cells: | Alveolar pneumonocytes, type II |
| Enzyme(s) | Trypsin: 0.1 % |
| Medium | HBSS, CMF |
Lung: Rat | |
| |
| Douglas, W., and Kaighn, M.: Clonal Isolation of Differentiated Rat Lung Cells
, In Vitro 10,
230, 1974 | |
| Cells: | Lung |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Moscona saline, CMF |
Lung: Rat | |
| |
| Kikkawa, Y., and Yoneda, K.: Type II Epithelial Cell of the Lung. I. Method of Isolation
, Lab Invest 30,
76, 1974 | |
| Cells: | Alveolar type II |
| Enzyme(s) | Trypsin: 1.0% |
| Medium | Joklik's medium |
Lymph nodes: Mouse | |
| |
| Terhorst, D., Chelbi, R., Wohn, C., Malosse, C., Tamoutounour, S., Jorquera, A., Bajenoff, M., Dalod, M., Malissen, B. and Henri, S.: Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis.
, J Immunol 195,
4953-61, 2015 | |
| Cells: | Dendritic |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.1% |
| Medium | RPMI 1640 |
Lymph nodes: Mouse | |
| |
| Dubrot, J., Duraes, F., Potin, L., Capotosti, F., Brighouse, D., Suter, T., LeibundGut-Landmann, S., Garbi, N., Reith, W., Swartz, M. and Hugues, S.: Lymph Node Stromal Cells Acquire Peptide-MHCII Complexes from Dendritic Cells and Induce Antigen-Specific CD4+T Cell Tolerance
, J Exp Med 211,
1153, 2014 | |
| Cells: | Stromal |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.004% |
| Medium | RPMI |
Lymph nodes: Mouse | |
| |
| Rayamajhi, M., Redente, E., Condon, T., Gonzalez-Juarrero, M., Riches, D. and Lenz, L.: Non-Surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry.
, J Vis Exp
51,
2702, 2011 | |
| Cells: | Lung draining |
| Enzyme(s) | Collagenase Type 4: 0.1-0.125% |
| Medium | HBSS |
Lymph nodes: Mouse | |
| |
| Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, Szakal AK: Follicular dendritic cell (FDC) precursors in primary lymphoid tissues
, J Immunol 160,
1078-84, 1998 | |
| Cells: | Follicular dentritic |
| Enzyme(s) | Collagenase Type 4: 0.25% Deoxyribonuclease I: 0.5% |
| Medium | HBSS |
Mammary: Bovine | |
| |
| Miranda, S., Wang, Y., Purdie, N., Osborne, V., Coomber, B. and Cant, J.: Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture.
, J Dairy Sci 92,
2670, 2009 | |
| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase Type 3: 400 u/ml Hyaluronidase: 100 u/ml Deoxyribonuclease I: 2 u/ml |
| Medium | HBSS |
Mammary: Bovine | |
| |
| Weber M., Purup S., Vestergaard M., Ellis S., Scndergard-Andersen J., Akers R., and Sejrsen K.: Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum
, J Endocrinol 161,
365, 1999 | |
| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase Type 2: 1% Hyaluronidase: 1% Deoxyribonuclease I: 0.03% |
| Medium | M-199 |
Mammary: Bovine | |
| |
| Gibson, C., Vega, J., Baumrucker, C., Oakley, C., and Welsch, C.: Establishment And Characterization Of Bovine Mammary Epithelial Cell Lines
, In Vitro Cell Dev Biol 27,
585, 1991 | |
| Cells: | Epithelial |
| Enzyme(s) | Hyaluronidase: 0.005% |
| Medium | HBSS |
Mammary: Bovine | |
| |
| Baumrucker, C., Deemer, K., Walsh, R., Riss, T., and Akers, R.: Primary Culture of Bovine Mammary Acini on a Collagen Matrix
, Tissue Cell
20 (4),
541, 1988 | |
| Cells: | Epithelial |
| Enzyme(s) | Deoxyribonuclease I: 0.04% |
| Medium | HBSS/Medium 199 |
Mammary: Bovine | |
| |
| Anderson, C., and Larson, B.: Comparative Maintenance of Function in Dispersed Cell and Organ Cultures
, Exp Cell Res
61,
24, 1970 | |
| Cells: | Mammary |
| Enzyme(s) | Collagenase: 0.30 % |
| Medium | HBSS or EBSS |
Mammary: Bovine | |
| |
| Schingoethe, D., Hageman, E., and Larson, B.: Essential Amino Acids for Milk Protein Synthesis in the In Vitro Secretory Cell and Stimulation by Elevated Levels
, Biochim Biophys Acta 148,
469, 1967 | |
| Cells: | Secretory Mammary gland |
| Enzyme(s) | Collagenase: 0.02 - 0.03% |
| Medium | HBSS or EBSS |
Mammary: Goat | |
| |
| Blanco, A., Rife, U., and Larson, B.: Lactate Dehydrogenase Isozymes during Dedifferentiation in Cultures of Mammary Secretory Cells
, Nature
214,
1331, 1967 | |
| Cells: | Mammary gland |
| Enzyme(s) | Collagenase: 0.02 - 0.03% |
| Medium | HBSS or EBSS |
Mammary: Guinea-Pig | |
| |
| Turba, F., and Hilpert, N.: Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German
, Biochem Z 334,
501, 1961 | |
| Cells: | Mammary gland |
| Enzyme(s) | Trypsin NF 1:250: 0.25% |
| Medium | Dulbecco phosphate |
Mammary: Human | |
| |
| Labarge, M., Garbe, J. and Stampfer, M.: Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture.
, J Vis Exp
71,
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| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase: 200 u/ml Hyaluronidase: 100 u/ml |
| Medium | DMEM/F-12 |
Mammary: Human | |
| |
| Ronnov-Jessen L., Villadsen R., Edwards J., and Petersen O.: Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts
, Am J Pathol 161,
471, 2002 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Collagenase Type 3: 900 u/ml |
| Medium | DMEM/F-12 |
Mammary: Human | |
| |
| Huss, F. and Kratz, G.: Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue.
, Cells Tissues Organs
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| Cells: | Mammary epithelial cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Mammary: Human | |
| |
| Ogmundsdottir, H., Petursdottir, I., Gudmundsdottir, I., Amundadottir, L., Ronnov-Jessen, L., and Petersen, O.: Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures
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936, 1993 | |
| Cells: | Epithelial Fibroblasts |
| Enzyme(s) | Collagenase Type 1: 450 IU/ml |
| Medium | DMEM/Ham's F-12 |
Mammary: Human | |
| |
| Ronnov-Jessen, L., VanDeurs, B., Nielsen, M., and Petersen, O.W.: Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture
, In Vitro Cell Dev Biol 28,
273, 1992 | |
| Cells: | Myofibroblasts |
| Enzyme(s) | Collagenase: 900 IU/ml |
| Medium | DME - F12 |
Mammary: Human | |
| |
| Berthon, P., Pancino, G., Cremoux, P., Roseto, A., Gespach, C., and Calvo, F.: Characterization of Normal Breast Epithelial Cells in Primary Cultures: Differentiation and Growth Factor Receptors Studies
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716, 1992 | |
| Cells: | Epithelial |
| Enzyme(s) | Hyaluronidase: 150 IU/ml |
| Medium | DMEM |
Mammary: Human | |
| |
| Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples
, In Vitro Cell Dev Biol 26,
1186, 1990 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 2.0% |
| Medium | DMEM/Ham's F-12 |
Mammary: Human | |
| |
| Leung, C., and Shiu, R.: Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix
, In Vitro 18,
476, 1981 | |
| Cells: | Tumor, breast |
| Enzyme(s) | Neuraminidase: 0.8 u/ml |
| Medium | HBSS |
Mammary: Human | |
| |
| Stampfer, M., Hallowes, R., and Hackett, A.: Growth of Normal Human Mammary Cells in Culture
, In Vitro 16 (5),
415, 1980 | |
| Cells: | Epithelial |
| Enzyme(s) | Hyaluronidase: 100 u/ml |
| Medium | DMEM/Ham's F-12 |
Mammary: Mouse | |
| |
| Liu, X., Johnson, S., Liu, S., Kanojia, D., Yue, W., Singh, U., Wang, Q, Wang Qi, Nie, Q. and Chen H.: Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy.
, Sci Rep
3,
2473, 2013 | |
| Cells: | Mammary tumor |
| Enzyme(s) | Collagenase: 0.15% Hyaluronidase: 0.020% |
| Medium | DMEM/F12 |
Mammary: Mouse | |
| |
| Cheng, N. and Lambert, D.: Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice.
, J Vis Exp
54,
e2716, 2011 | |
| Cells: | Fibroblasts, carcinoma |
| Enzyme(s) | Collagenase Type 4: 0.5% Trypsin: 0.2% Deoxyribonuclease I: 0.004 Hyaluronidase: 1,000 u/ml |
| Medium | DMEM |
Mammary: Mouse | |
| |
| Taddei, I., Deugnier, M., Faraldo, M., Petit, V., Bouvard, D., Medina, D., Fassler, R., Thiery, J. and Glukhova, M.: Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells
, Nat Cell Biol 10,
716-22, 2008 | |
| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase: 0.3% Hyaluronidase: 100 u/ml Trypsin: 0.25% Neutral Protease: 0.5% Deoxyribonuclease I: 0.01% |
| Medium | see reference |
Mammary: Mouse | |
| |
| Mueller, S., Clark, J., Myers, P. and Korach, K.: Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha.
, Endocrinology 143,
2357, 2002 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 3: 25 u/ml Hyaluronidase: 0.1% Protease XIV: 0.05% Deoxyribonuclease I: 0.04% |
| Medium | DMEM/F12 |
Mammary: Mouse | |
| |
| Boulanger CA, Smith GH: Reducing mammary cancer risk through premature stem cell senescence
, Oncogene
20,
2264-72, 2001 | |
| Cells: | Mammary epithelial stem |
| Enzyme(s) | Collagenase Type 3: 0.1% Hyaluronidase: 0.1% Pronase: 1.25% Deoxyribonuclease I: 0.2% |
| Medium | DMEM |
Mammary: Mouse | |
| |
| Kanazawa, T., and Hosick, H.: Transformed Growth Phenotype of Mouse Mammary Epithelium in Primary Culture Induced by Specific Fetal Mesenchymes
, J Cell Physiol 153,
381, 1992 | |
| Cells: | Epithelium |
| Enzyme(s) | Collagenase: 250 u/ml |
| Medium | HBSS |
Mammary: Mouse | |
| |
| Beck, J., Hosick, H., and Watkins, B.: Growth of Epithelium From a Preneoplastic Mammary Outgrowth on Response to Mammary Adipose Tissue
, In Vitro Cell Dev Biol 25 (5),
409, 1989 | |
| Cells: | Adipocytes |
| Enzyme(s) | Trypsin: 50 µg/ml |
| Medium | DMEM |
Mammary: Mouse | |
| |
| Bandyopadhyay, G., Imagawa, W., Wallace, D., and Nandi, S.: Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture
, J Biol Chem 263,
7567, 1988 | |
| Cells: | Epithelial |
| Enzyme(s) | Pronase: 0.01% |
| Medium | Hepes buffered medium 199 |
Mammary: Mouse | |
| |
| Ehmann, U., Peterson, W., and Misfeldt, D.: To Grow Mouse Mammary Epithelial Cells in Culture
, J Cell Biol
98,
1026, 1984 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | DMEM |
Mammary: Mouse | |
| |
| Taketani, Y., and Oka, T.: EGF Stimulates Cell Proliferation and Inhibits Functional Differentiation of Mouse Mammalian Dipithelial Cells in Culture
, Endocrinology 113,
871, 1983 | |
| Cells: | Epithelial |
| Enzyme(s) | Deoxyribonuclease I: 0.0001% |
| Medium | Medium 199 |
Mammary: Mouse | |
| |
| Riser, M., Huff, B., and Medina, D.: Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells
, In Vitro 19,
730, 1983 | |
| Cells: | Epithelial |
| Enzyme(s) | Pepsin: 0.1% and 0.05% Pepsin: 0.1% |
| Medium | HBSS with 0.2% EDTA, CMF |
Mammary: Mouse | |
| |
| Jones, W., and Hallowes, R.: Isolation of the Epithelial Subcomponents of the Mouse Mammary Gland for Tissue-Level Culture Studies, J Tiss Cul Meth 8 (1),
17, 1983 | |
| Cells: | Epithelial |
| Enzyme(s) | Deoxyribonuclease I: 0.1% |
| Medium | DMEM |
Mammary: Mouse | |
| |
| Ehmann, U., and Misfeldt, D.: Mouse Mammary Cells in D-Valine Medium
, In Vitro 18,
407, 1982 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | DMEM |
Mammary: Mouse | |
| |
| Asch, B., Burstein, N., Vidrich, A., and Sun, T.: Identification of Mouse Mammary Epithelial Cells by Immunofluorescence With Rabbit and Guinea-Pig Antikeratin Antisera
, Proc Natl Acad Sci U S A 78,
5643, 1981 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | HBSS/DMEM |
Mammary: Mouse | |
| |
| Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels
, In Vitro 16,
502, 1980 | |
| Cells: | Mammary tumors Epithelial |
| Enzyme(s) | Collagenase: 1.0% |
| Medium | HBSS |
Mammary: Mouse | |
| |
| Yang J, Richards J, Guzman R, Imagawa W, and Nandi S.: Sustained growth in primary culture of normal mammary epithelial cells embedded in collagen gels
, Proc Natl Acad Sci U S A 77,
2088, 1980 | |
| Cells: | Mammary |
| Enzyme(s) | (see reference): |
| Medium | HBSS |
Mammary: Mouse | |
| |
| White, M., Hu, A., Hamamoto, S. and Nandi: In vitro analysis of proliferating epithelial cell populations from the mouse mammary gland: fibroblast-free growth and serial passage
, In Vitro 14,
271, 1978 | |
| Cells: | Epithelial Mammary gland |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | HBSS |
Mammary: Mouse | |
| |
| Ceriani, R., Peterson, J., and Abraham, S.: Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells
, In Vitro 14,
887, 1978 | |
| Cells: | Mammary |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | CMF medium |
Mammary: Mouse | |
| |
| DeOme, K., Miyamoto, M., Osborn, R., Guzman, R., and Lum, K.: Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice
, Cancer Res 38,
2103, 1978 | |
| Cells: | Nodule-transformed |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Medium 199 |
Mammary: Mouse | |
| |
| Asch, B., and Medina, D.: Concanavalin A-Induced Agglutinability of Normal, Preneoplastic, and Neoplastic Mouse Mammary Cells
, J Natl Cancer Inst 61 (6),
1423, 1978 | |
| Cells: | Swiss 3T3 |
| Enzyme(s) | Collagenase Type 3: 0.2% |
| Medium | PBS CMF |
Mammary: Mouse | |
| |
| Emerman, J., Enami, J., Pitelka, D., and Nandi, S.: Hormonal Effects on Intracellular and Secreted Casein in Cultures of Mouse Mammary Epithelial Cells on Floating Collagen Membranes
, Proc Natl Acad Sci U S A 74 (10),
4466, 1977 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 3: 0.12% |
| Medium | HBSS |
Mammary: Mouse | |
| |
| Kerkof, P., and Abraham, S.: Preparation of Adipose Cell-Free Suspensions of Mammary Gland Parenchymal Cells from Lactating Mice, Methods Enzymol
69,
693, 1976 | |
| Cells: | Parenchymal |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | Kreb's Ringer bicarbonate buffer |
Mammary: Mouse | |
| |
| Moore, D., and Lasfargues, E.: Method for the Continuous Cultivation of Mammary Epithelium
, In Vitro 7,
21, 1971 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Eagle's MEM |
Mammary: Mouse | |
| |
| Prop, F., and Wiepjes, G.: Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse
, Exp Cell Res
61,
451, 1970 | |
| Cells: | Mammary |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | BSS, CMF |
Mammary: Mouse | |
| |
| Pitelka, D., Kerkof, P., Gagne, H., Smith, S., and Abraham, S.: Characteristics of Cells Dissociated from Mouse Mammary Glands. I. Method of Separation and Morphology of Parenchymal Cells from Lactating Glands
, Exp Cell Res
57,
43, 1969 | |
| Cells: | Parenchymal |
| Enzyme(s) | Collagenase: 0.33% |
| Medium | Kreb's buffer |
Mammary: Mouse | |
| |
| Kopelovich, L., Abraham, S., McGrath, H., DeOme, K., Chaikoff, I.: Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland
, Cancer Res 26,
1534, 1966 | |
| Cells: | Mammary |
| Enzyme(s) | Trypsin NF 1:250: 0.25% |
| Medium | HBSS |
Mammary: Mouse | |
| |
| Daniel, C., and DeOme, K.: Growth of Mouse Mammary Glands In Vivo After Monolayer Culture
, Science 149,
634, 1965 | |
| Cells: | Mammary |
| Enzyme(s) | Collagenase: 0.05% - 0.1% |
| Medium | HBSS |
Mammary: Mouse | |
| |
| Lasfargues, E.: Cultivation and Behavior In Vitro of the Normal Mammary Epithelium of the Adult Mouse. II. Observations on the Secretory Activity
, Exp Cell Res
13,
553, 1957 | |
| Cells: | Epithelial Mammary |
| Enzyme(s) | Collagenase: 0.02% |
| Medium | Simm's |
Mammary: Rat | |
| |
| Mei, N., McDaniel, L., Dobrovolsky, V., Guo, X., Shaddock, J., Mittelstaedt, R., Azuma, M., Shelton, S., McGarrity, L., Doerge, D. and Heflich, R.: The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats.
, Toxicol Sci 115,
412, 2010 | |
| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase Type 3: 0.35% |
| Medium | HBSS |
Mammary: Rat | |
| |
| Maffini M., Soto A., Calabro J., Ucci A., and Sonnenschein C.: The Stroma as a Crucial Target in Rat Mammary Gland Carcinogenesis
, J Cell Sci
117,
1495, 2004 | |
| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase Type 3: 0.15% |
| Medium | DMEM/F12 |
Mammary: Rat | |
| |
| Djuric Z., Lewis S., Lu M., Mayhugh M., Naegeli L., Tang N., and Hart R.: Effect of Varying Caloric Restriction Levels on Female Rat Growth and 5-hydroxymethyl-2'-deoxyuridine in DNA
, Toxicol Sci 66,
125, 2002 | |
| Cells: | Mammary gland epithelial |
| Enzyme(s) | Collagenase Type 3: 0.35% |
| Medium | see reference |
Mammary: Rat | |
| |
| Brake P., Zhang L., and Jefcoate C.: Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids
, Mol Pharmacol 54,
825, 1998 | |
| Cells: | Mammary fibroblasts |
| Enzyme(s) | Collagenase Type 3: 0.2% Neutral Protease: 0.2% Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F-12 |
Mammary: Rat | |
| |
| Varela L., Darcy K., and Ip M.: The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells
, Endocrinology 138,
3891, 1997 | |
| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase Type 3: 0.2% Neutral Protease: 0.2% |
| Medium | DMEM/F12 |
Mammary: Rat | |
| |
| Soriano, J., Pepper, M., Nakamura, T., Orci, L., and Montesano, R.: Hepatocyte Growth Factor Stimulates Extensive Development of Branching Duct-like Structures by Cloned Mammary Gland Epithelial Cells
, J Cell Sci
108,
413-430, 1995 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 1: 0.4% |
| Medium | DMEM |
Mammary: Rat | |
| |
| Laduca, J., and Sinha, D.: In Vitro Carcinogenesis of Mammary Epithelial Cells by N-Nitroso-N-Methylurea Using a Collagen Gel Matrix Culture
, In Vitro Cell Dev Biol 29A,
789, 1993 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 3: 0.5% |
| Medium | EBSS |
Mammary: Rat | |
| |
| Lin, T., Hom, Y.K., Richards, J. and Nandi, S.: Effects of Antioxidants and Reduced Oxygen Tension on Rat Mammary Epithelial Cells in Culture
, In Vitro Cell Dev Biol 27A,
191, 1991 | |
| Cells: | Epithelial Mammary |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | Medium 199 |
Mammary: Rat | |
| |
| Ehmann, U., Osborn, R., Guzman, R., and Fajardo, L.: Cultured Proliferating Rat Mammary Epithelial Cells
, In Vitro Cell Dev Biol 27,
749, 1991 | |
| Cells: | Epithelial |
| Enzyme(s) | Neutral Protease: 3 u/ml |
| Medium | Medium 199 |
Mammary: Rat | |
| |
| Hahm, H.A., Ip, M.M.: Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors
, In Vitro Cell Dev Biol 26,
791, 1990 | |
| Cells: | Epithelial |
| Enzyme(s) | Neutral Protease: 0.2% |
| Medium | EBSS |
Mammary: Rat | |
| |
| McGrath, M., Palmer, S., and Nandi, S.: Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF
, J Cell Physiol 125,
182, 1985 | |
| Cells: | Epithelial |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Medium 199 |
Mammary: Rat | |
| |
| Ethler, S.: Primary Culture and Serial Passage of Normal and Carcinogen-Treated Rat Mammary Epithelial Cells In Vitro
, J Natl Cancer Inst 74 (6),
1307, 1985 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Medium 199 |
Mammary: Rat | |
| |
| Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma
, In Vitro 18,
565, 1982 | |
| Cells: | Epithelial, cancer and tumor |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Eagles's MEM |
Mammary: Rat | |
| |
| Richards, J., and Nandi, S.: Primary Culture of Rat Mammary Epithelial Cells. I. Effect of Plating Density, Horomones, and Serum on DNA Synthesis
, J Natl Cancer Inst 61 (3),
765, 1978 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | Medium 199 |
Mammary: Rat | |
| |
| Katz, J., Wals, P. and Van de Velde, R: Lipogenesis by Acini from Mammary Gland of Lactating Rats
, J Biol Chem 249,
7348, 1974 | |
| Cells: | Acini |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | HBSS |
Mammary: Rat | |
| |
| Martin, R., and Baldwin, R.: Effects of Insulin on Isolated Rat Mammary Cell Metabolism: Glucose Utilization and Metabolite Patterns
, Endocrinology 89,
1263, 1971 | |
| Cells: | Mammary |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Kreb's Ringer bicarbonate buffer |
Mammary: Rat | |
| |
| Moon, R., Janns, D., and Young, S.: Preparation of Fat Cell-"Free" Rat Mammary Gland
, J Histochem Cytochem 17 (3),
182, 1969 | |
| Cells: | Mammary |
| Enzyme(s) | Collagenase: 0.35% |
| Medium | Medium 199 |
Miscellaneous: Bovine | |
| |
| Lee, J., Cheung, K. and Leung, V.: Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability.
, J Orthop Res 33,
1743-55, 2015 | |
| Cells: | Nucleus pulposus |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Miscellaneous: Equine | |
| |
| Nemoto, M., Kizaki, K., Yamamoto, Y., Oonuma, T. and Hashizume, K.: Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration.
, J Equine Sci
24,
17-24, 2013 | |
| Cells: | Tendon Cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Miscellaneous: Goat | |
| |
| Arora, A., Sriram, M., Kothari, A. and Katti, D.: Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering.
, Cytotherapy
19,
881-894, 2017 | |
| Cells: | Infrapatellar fat pad mesenchymal stromal |
| Enzyme(s) | Collagenase: 0.15% |
| Medium | DMEM |
Miscellaneous: Human | |
| |
| Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation.
, Nat Commun
11,
4061, 2020 | |
| Cells: | Adhesion fibroblasts |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | Medium 199 |
Miscellaneous: Human | |
| |
| Sun, Y., Lv, M., Zhou, L., Tam, V., Lv, F., Chan, D., Wang, H., Zheng, Z., Cheung, K. and Leung, V.: Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation.
, Osteoarthritis Cartilage
23,
1194-203, 2015 | |
| Cells: | Nucleus pulposus |
| Enzyme(s) | Pronase: 0.25% Collagenase Type 2: 600 u/ml |
| Medium | DMEM |
Miscellaneous: Human | |
| |
| Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia.
, PLoS ONE
8,
e56749, 2013 | |
| Cells: | Dental pulp stem cells |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Miscellaneous: Human | |
| |
| Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium.
, Front Physiol
4,
357, 2013 | |
| Cells: | Dental pulp stem |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM/F12 |
Miscellaneous: Human | |
| |
| Lee, Y., Lee, J., Park, H., Lim, Y., Lee, J., Wang, S. and Lee, B.: Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue.
, Cell Physiol Biochem 31,
513-24, 2013 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | PBS |
Miscellaneous: Human | |
| |
| Ozdener, H., Spielman, A. and Rawson, N.: Isolation and Culture of Human Fungiform Taste Papillae Cells
, J Vis Exp
63,
e3730, 2012 | |
| Cells: | Fungiform taste papillae |
| Enzyme(s) | Collagenase Type 1: 550 u/ml Elastase: 10 u/ml Soybean Trypsin Inhibitor: 0.09% |
| Medium | see reference |
Miscellaneous: Human | |
| |
| Pauly, S., Klatte, F., Strobel, C., Schmidmaier, G., Greiner, S. and Scheibel, M. Wildemann B: Characterization of Tendon Cell Cultures of the Human Rotator Cuff.
, Eur Cell Mater
20,
84-97, 2010 | |
| Cells: | Tendon cells |
| Enzyme(s) | Collagenase Type 2: 0,3% |
| Medium | DMEM/Hams F12 |
Miscellaneous: Human | |
| |
| Panetta, N., Gupta, D., Kwan, M., Wan, D., Commons, G. and Longaker, M.: Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells.
, Plast Reconstr Surg
124,
65, 2009 | |
| Cells: | Adipose derived stromal |
| Enzyme(s) | Collagenase Type 2: 0.075% |
| Medium | DMEM |
Miscellaneous: Human | |
| |
| Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling.
, Stem Cells 25,
628-38, 2007 | |
| Cells: | Oral epithelial |
| Enzyme(s) | Neutral Protease: 1.2 u/ml Trypsin: 0.05% |
| Medium | PBS |
Miscellaneous: Human | |
| |
| Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM: Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin
, Circ Res 98,
1282-9, 2006 | |
| Cells: | Carotid artery plaque macrophage |
| Enzyme(s) | Collagenase Type 4: 450 u/ml Deoxyribonuclease I: 500 u/ml Soybean Trypsin Inhibitor: 0.1% |
| Medium | HBSS |
Miscellaneous: Human | |
| |
| Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation.
, Am J Physiol Gastrointest Liver Physiol
285,
G1277, 2003 | |
| Cells: | Esophageal microvascular endothelial |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | MCDB-131 |
Miscellaneous: Human | |
| Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22,
133, 2000 | |
| Cells: | Gastric epithelial cells |
| Enzyme(s) | Collagenase Type 2: 200 u/ml Neutral Protease: 1.2 u/ml Soybean Trypsin Inhibitor: 0.125% |
| Medium | L-15 |
Miscellaneous: Human | |
| |
| Chen V, Croft D, Purkis P, Kramer IM: Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release.
, Br J Rheumatol 37,
148-56, 1998 | |
| Cells: | Synoviocytes |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM/F12 |
Miscellaneous: Human | |
| |
| Lanas. A., Nerin, J., Esteva, F. and Sainz, R.: Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells.
, Gut 36,
657-63, 1995 | |
| Cells: | Peptic cells |
| Enzyme(s) | Collagenase Type 4: 0.1% Soybean Trypsin Inhibitor: 0.2% |
| Medium | Ringer solution |
Miscellaneous: Human | |
| |
| Stern MH, Dreizen S, Mackler BF, Levy BM: Isolation and characterization of inflammatory cells from the human periapical granuloma
, J Dent Res 61,
1408-12, 1982 | |
| Cells: | Periapical granuloma |
| Enzyme(s) | CLSPA: 0.25% |
| Medium | RPMI-1640 |
Miscellaneous: Insect | |
| |
| Goodwin, R and McCawley, P: Initiating Attached Cell Lines From the Lepidoptera (Insecta), Meth Cell Sci 3,
567, 1977 | |
| Cells: | Lepitopteran |
| Enzyme(s) | Collagenase Type 3: 0.35% Hyaluronidase: 0.01% |
| Medium | Dulbecco PBS |
Miscellaneous: Invertebrate | |
| |
| Raman, M. and Mathew, S.: Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny.
, J Food Sci Technol
51,
1509-16, 2014 | |
| Cells: | Mantle |
| Enzyme(s) | Collagenase: see reference Papain: see reference |
| Medium | Phosphate buffer |
Miscellaneous: Mouse | |
| |
| Huang, L., Desai, R., Conrad, D., Leite, N., Akshinthala, D., Lim, C., Gonzalez, R., Muthuswamy, L., Gartner, Z. and Muthuswamy, S.: Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids.
,Cell Stem Cell Vol. 28,, ,
1090-1104.e6, 2021 | |
| Cells: | Organoid cultures |
| Enzyme(s) | Stemxyme 1: 0.1% |
| Medium | PBS |
Miscellaneous: Mouse | |
| |
| He, M., Wu, B., Ye, W., Le, D., Sinclair, A., Padovano, V., Chen, Y., Li, K., Sit, R., Tan, M., Caplan, M., Neff, N., Jan, Y., Darmanis, S. and Jan, L.: Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway.
, Elife
9,
53085, 2020 | |
| Cells: | Trachea |
| Enzyme(s) | Elastase: 0.1% Neutral Protease: 0.25% Deoxyribonuclease I: 0.01% Trypsin: 0.125% |
| Medium | DMEM/F12 |
Miscellaneous: Mouse | |
| |
| Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation.
, Nat Commun
11,
4061, 2020 | |
| Cells: | Adhesion fibroblasts |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | Medium 199 |
Miscellaneous: Mouse | |
| |
| Atta, H., Gheinani, A., Wacker, A., Heshmati, Y., Bigger-Allen, A., Lambrinos, G., Gao, Y., Bielenberg, D. and Adam, R.: A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury.
, J Vis Exp
,
, 2020 | |
| Cells: | Urinary bladder |
| Enzyme(s) | Collagenase Type 1: 50 u/ml Collagenase Type 3: 40 u/ml Papain: 45 u/ml Deoxyribonuclease I: 4 u/ml Collagenase Type 6: 20 u/ml |
| Medium | Tyrode solution |
Miscellaneous: Mouse | |
| |
| Huang, C., Chen, C. and Shyr, C.: The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer.
, Cytotherapy
19,
1233-1245, 2017 | |
| Cells: | Urothelial |
| Enzyme(s) | Collagenase Type 1: 0.5% |
| Medium | PBS |
Miscellaneous: Mouse | |
| |
| Ishida, T., Suzuki, S., Lai, C., Yamazaki, S., Kakuta, S., Iwakura, Y., Nojima, M., Takeuchi, Y., Higashihara, M., Nakauchi, H. and Otsu, M.: Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells.
, Stem Cells 35,
989-1002, 2017 | |
| Cells: | Bone marrow stromal |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | PBS |
Miscellaneous: Mouse | |
| |
| Kim, Y., Wang, S., Tymanskyj, S., Ma, L., Tao, H. and Zhang, L.: Dcc Mediates Functional Assembly of Peripheral Auditory Circuits.
, Sci Rep
6,
23799, 2016 | |
| Cells: | Cochlear |
| Enzyme(s) | Papain: 20 u/ml Trypsin: 0.05% |
| Medium | DMEM |
Miscellaneous: Mouse | |
| |
| Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor.
, J Vis Exp
,
, 2014 | |
| Cells: | Epithelial stem |
| Enzyme(s) | Collagenase: 2% |
| Medium | DMEM/F12 |
Miscellaneous: Mouse | |
| |
| Smith, T., Grider, J., Dewey, W. and Akbarali, H.: Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels.
, PLoS ONE
7,
e45251, 2012 | |
| Cells: | Myenteric plexus |
| Enzyme(s) | Collagenase Type 2: 0.13% |
| Medium | Neurobasal A |
Miscellaneous: Mouse | |
| |
| Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium.
, PLoS ONE
7,
e45517, 2012 | |
| Cells: | Synovial mesenchymal |
| Enzyme(s) | Collagenase: 0.1% Deoxyribonuclease I: 0.005% |
| Medium | DMEM |
Miscellaneous: Mouse | |
| |
| Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands.
, Immunity
34,
590, 2011 | |
| Cells: | Spleen, bone marrow endothelial |
| Enzyme(s) | Collagenase Type 4: 0.3-1.0% Deoxyribonuclease I: 20 u/ml |
| Medium | PBS |
Miscellaneous: Mouse | |
| |
| Stock, A., Booth, S. and Cerundolo, V.: Prostaglandin E2 Suppresses the Differentiation of Retinoic Acid-Producing Dendritic Cells in Mice and Humans.
, J Exp Med 208,
761, 2011 | |
| Cells: | Dentritic |
| Enzyme(s) | Collagenase Type 2: 0.1% Deoxyribonuclease I: 0.001% |
| Medium | DME |
Miscellaneous: Mouse | |
| |
| Jan, T., Chai, R., Sayyid, Z. and Cheng, A.: Isolating LacZ-Expressing Cells from Mouse Inner Ear Tissues Using Flow Cytometry.
, J Vis Exp
58,
e3432, 2011 | |
| Cells: | Cochleaer |
| Enzyme(s) | Trypsin: 0.125% |
| Medium | DMEM/F-12 |
Miscellaneous: Mouse | |
| |
| Xu, S., De Becker, A., Van Camp, B., Vanderkerken, K. and Van Riet, I.: An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells.
, J Biomed Biotechnol
2010,
105940, 2010 | |
| Cells: | Bone marrow mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.25% |
| Medium | RPMI 1640 |
Miscellaneous: Mouse | |
| |
| Staszkiewicz, J., Gimble, J., Manuel, J. and Gawronska-Kozak, B.: IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential.
, Stem Cells 26,
2666, 2008 | |
| Cells: | Ear mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F12 |
Miscellaneous: Mouse | |
| |
| Ji, H., Rintelen, F., Waltzinger, C., Meier, D, Bilancio, A., Pearce, W., Hirsch, E., Wymann, M., Ruckle, T., Camps, M., Vanhaesebroeck, B., Okkenhaug, K. and Rommel, C.: Inactivation of PI3Kgamma and PI3Kdelta Distorts T-cell Development and Causes Multiple Organ Inflammation.
, Blood 110,
2940, 2007 | |
| Cells: | Salivary gland and stomach |
| Enzyme(s) | Collagenase Type 4: 0.8% Deoxyribonuclease I: 1.0% |
| Medium | RPMI-1640 |
Miscellaneous: Mouse | |
| |
| Bertoncello, I. and Williams, B.: Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining.
, Methods Mol Biol 263,
181, 2004 | |
| Cells: | Bone marrow |
| Enzyme(s) | Collagenase Type 1: 0.15% Neutral Protease: 0.15% |
| Medium | PBS |
Miscellaneous: Mouse | |
| |
| Minamoto Kanji, Pinsky DavidJ: Recipient iNOS but not eNOS deficiency reduces luminal narrowing in tracheal allografts
, J Exp Med 196,
1321-33, 2002 | |
| Cells: | Tracheal inflammatory cells |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 50 u/ml Soybean Trypsin Inhibitor: 0.1% |
| Medium | RPMI 1640 |
Miscellaneous: Porcine | |
| |
| Kean, T and Dennis, J.: Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions.
, PLoS ONE
10,
e0129961, 2015 | |
| Cells: | Synoviocytes |
| Enzyme(s) | Hyaluronidase: 660 u/ml Trypsin: 0.25% Collagenase Type 2: 583 u/ml |
| Medium | DMEM |
Miscellaneous: Porcine | |
| |
| Potier, E. and Ito. K.: Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System.
, Tissue Eng Part A
20,
3241-51, 2014 | |
| Cells: | Nucleus pulposus |
| Enzyme(s) | Collagenase: 0.05% Pronase: 0.3% |
| Medium | DMEM |
Miscellaneous: Porcine | |
| |
| Cho, H., Park, S., Lee, S., Kang, M., Hasty, K. and Kim, S.: Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms.
, Exp Mol Med
43,
334-40, 2011 | |
| Cells: | Nucleous pulposus and AF |
| Enzyme(s) | Pronase: 0.05% Collagenase: 0.2% |
| Medium | Modified F-12K |
Miscellaneous: Rabbit | |
| |
| Zhang, J. and Wang, J.: Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes.
, BMC Musculoskelet Disord
11,
10, 2010 | |
| Cells: | Tenocytes and tendon stem cells |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Miscellaneous: Rat | |
| |
| Jain, S., Tran, T. and Amiji, M.: Macrophage Repolarization with Targeted Alginate Nanoparticles Containing IL-10 Plasmid DNA for the Treatment of Experimental Arthritis.
, Biomaterials 61,
162-77, 2015 | |
| Cells: | Synovial macrophages |
| Enzyme(s) | Collagenase Type 1: 250 u/ml |
| Medium | DMEM |
Miscellaneous: Rat | |
| |
| Zhou, B., Zhou, Y. and Tang, K.: The Effects of Substance P on Pluripotent Tendon Cells: an In Vitro and In Vivo Study.
, J Musculoskelet Neuronal Interact
14,
349-58, 2014 | |
| Cells: | Pluripotent tendon |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Miscellaneous: Rat | |
| |
| Kaneko, N., Rikimaru, T., Fujimura, T., Mori, S., Hidaka, S. and Kaya, H.: Preparation of Rat Gingival Mitochondria with an Improved Isolation Method.
, Int J Dent
2010,
275103, 2010 | |
| Cells: | Gingival mitochondria |
| Enzyme(s) | Collagenase Type 1: 0.115-0.130 % |
| Medium | HBSS |
Miscellaneous: Rat | |
| |
| Silver, N., Proctor, GB, Arno, M. and Carpenter GH: Activation of mTOR Coincides with Autophagy During Ligation-induced Atrophy in the Rat Submandibular Gland.
, Cell Death Dis
1,
e14, 2010 | |
| Cells: | Submandibular acinar |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | PBS |
Miscellaneous: Rat | |
| |
| Lin, A., Hokugo, A., Choi, J. and Nishimura, I.: Small Cytoskeleton-Associated Molecule, Fibroblast Growth Factor Receptor 1 Oncogene Partner 2/Wound Inducible Transcript-3.0 (FGFR1OP2/wit3.0), Facilitates Fibroblast-Driven Wound Closure.
, Am J Pathol 176,
108-21, 2010 | |
| Cells: | Oral fibroblasts |
| Enzyme(s) | Collagenase Type 1: 200 u/ml |
| Medium | DMEM |
Miscellaneous: Rat | |
| |
| Moghaddami, M., Cleland, L. and Mayrhofer, G.: MHC II+ CD45+ Cells from Synovium-Rich Tissues of Normal Rats: Phenotype, Comparison with Macrophage and Dendritic Cell Lineages and Differentiation into Mature Dendritic Cells In Vitro.
, Int Immunol 17,
1103, 2005 | |
| Cells: | Synovial cells |
| Enzyme(s) | Collagenase Type 1: 250 u/ml |
| Medium | RPMI 1640 |
Muscle: Bovine | |
| |
| Lee, E., Choi, J., Hyun, J., Cho, K., Hwang, I., Lee, H., Chang, J. and Choi, I.: Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells., AJAS
20,
501, 2007 | |
| Cells: | Muscle satellite cells |
| Enzyme(s) | Collagenase Type 2: 300 u/ml |
| Medium | Krebs-Ringer Bicarbonate |
Muscle: Bovine | |
| |
| Yu, M., McAndrew, R., Al-Saghir, R., Maier, K., Medhora, M., Roman, R. and Jacobs, E.: Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries.
, J Appl Physiol 93,
1391, 2002 | |
| Cells: | Pulmonary artery endothelial and smooth muscle cells |
| Enzyme(s) | Collagenase: 0.04-0.05% Soybean Trypsin Inhibitor: 0.04% |
| Medium | RPMI-1640 |
Muscle: Bovine | |
| |
| Absher, M., Woodcock-Mitchell, J., Mitchell, J., Baldor, L., Low, R., and Warshaw, D.: Characterization of Vascular Smooth Muscle Cell Phenotype in Long-Term Culture
, In Vitro Cell Dev Biol 25 (2),
183, 1989 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Muscle: Bovine | |
| |
| Warshaw, D., Szarek, J., Hubbard, M., and Evans, J.: Pharmacology and Force Development of Single Freshly Isolated Bovine Cartoid Artery Smooth Muscle Cells
, Circ Res 58,
399, 1986 | |
| Cells: | Vascular smooth muscle |
| Enzyme(s) | Elastase Type 3: 50 u/ml |
| Medium | PSS |
Muscle: Bovine | |
| |
| Davies, P. and Kerr, C.: Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts
, Biochim Biophys Acta 712,
26, 1982 | |
| Cells: | Smooth muscle, fibroblasts |
| Enzyme(s) | Trypsin: 0.055% |
| Medium | DMEM |
Muscle: Canine | |
| |
| Parker, M., Loretz, C., Tyler, A., Snider, L., Storb. R. and Tapscott, S.: Inhibition of CD26/DPP-IV Enhances Donor Muscle Cell Engraftment and Stimulates Sustained Donor Cell Proliferation.
, Skelet Muscle
2,
4, 2012 | |
| Cells: | Skeletal muscle |
| Enzyme(s) | Collagenase Type 4: 200 u/ml Neutral Protease: 1 u/ml |
| Medium | DMEM |
Muscle: Canine | |
| |
| Subramanian, M., Madden, J., and Harder, D.: A Method for the Isolation of Cells from Arteries of Various Sizes, J Tiss Cul Meth 13,
13, 1991 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Elastase: 50 u/ml |
| Medium | PSS |
Muscle: Canine | |
| |
| Wilde, D., and Lee, K.: Outward Potassium Currents in Freshly Isolated Smooth Muscle Cell of Dog Coronary Arteries
, Circ Res 65,
1718, 1989 | |
| Cells: | Smooth muscle Vascular |
| Enzyme(s) | Elastase: 34 u/ml |
| Medium | Tyrode's solution w/ calcium |
Muscle: Canine | |
| |
| Dobrin, P., and Canfield, T.: Elastase, Collagenase, and the Biaxial Elastic Properties of Dog Carotid Artery
, Am J Physiol Heart Circ Physiol
247 (16),
H124, 1984 | |
| Cells: | Artery Carotid |
| Enzyme(s) | Elastase: 80 u/ml |
| Medium | PSS |
Muscle: Chicken | |
| |
| Dirksen W., Vladic F., and Fisher S.: A Myosin Phosphatase Targeting Subunit Isoform Transition Defines a Smooth Muscle Developmental Phenotypic Switch
, Am J Physiol/Cell 278(3),
C589, 2000 | |
| Cells: | Gizzard and aorta smooth muscle |
| Enzyme(s) | Collagenase Type 1: 0.15% |
| Medium | HBSS |
Muscle: Chicken | |
| |
| Chamley-Campbell, J., Campbell, G., and Ross, R.: The Smooth Muscle in Cell Culture
, Physiol Res 59,
1, 1979 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Trypsin: 0.05% - 0.1% |
| Medium | HBSS |
Muscle: Chicken | |
| |
| Bullaro, J., and Brookman, D.: Comparison of Skeletal Muscle Monolayer Cultures Initiated With Cells Dissociated by the Vortex and Trypsin Methods
, In Vitro 12,
564, 1976 | |
| Cells: | Muscle |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Puck's saline A |
Muscle: Chicken | |
| |
| Tepperman, K., Morris, G., Essien, F., and Heywood, S.M.: A Mechanical Dissociation Method For Preparation of Muscle Cell Cultures
, J Cell Physiol 86,
561, 1975 | |
| Cells: | Muscle |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Saline G |
Muscle: Chicken | |
| |
| Hilfer, S., and Brown, J.: Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart
, Exp Cell Res
65,
246, 1971 | |
| Cells: | Thyroid Muscle Heart |
| Enzyme(s) | Collagenase: 0.25% |
| Medium | Tyrode's saline, potassium free |
Muscle: Chicken | |
| |
| Hilfer, S.: Collagenase Treatment of Chick Heart and Thyroid,Tissue Cult Methods & Applications,Kruse, P., and Patterson, M., ,
246, 1971 | |
| Cells: | Muscle |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | CMF HBSS |
Muscle: Chicken | |
| |
| Rinaldini, L.: An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells
, Exp Cell Res
16,
477, 1959 | |
| Cells: | Various tissues (heart, liver, skeletal, cardiac) |
| Enzyme(s) | Trypsin: various grades |
| Medium | CMF Tyrode's solution |
Muscle: Feline | |
| |
| Madden, J., Vadula, M., and Kurup, V.: Effects of Hypoxia and Other Vasoactive Agents on Pulmonary and Cerebral Artery Smooth Muscle Cells
, Am J Physiol 263,
L384, 1992 | |
| Cells: | Cerebral arteries |
| Enzyme(s) | Elastase: 50 u/ml |
| Medium | Puck's solution |
Muscle: Feline | |
| |
| Follmer, C.H., Ten Eick, R.E., and Yeh, J.Z.: Sodium Current Kinetics in Cat Atrial Myocytes, J Physiol
384,
169, 1987 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase: 0.12% |
| Medium | Kreb's Henseleit, CF |
Muscle: Fish | |
| |
| Alexander, M., Kawahara, G., Kho, A., Howell, M., Pusack, T., Myers, J., Montanaro, F., Zon, L., Guyon, J. and Kunkel, L.: Isolation and Transcriptome Analysis of Adult Zebrafish Cells Enriched for Skeletal Muscle Progenitors.
, Muscle Nerve
43,
741-50, 2011 | |
| Cells: | Myogenic progenitor |
| Enzyme(s) | Collagenase Type 4: 0.5% Neutral Protease: 1.2 u/ml |
| Medium | PBS |
Muscle: Fish | |
| |
| Mitra, R. and Morad, M.: A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates
, Am J Physiol 249,
H1056, 1985 | |
| Cells: | Myocytes, heart and stomach |
| Enzyme(s) | Protease XIV: 0.028% |
| Medium | Solution C (see reference) |
Muscle: Frog | |
| |
| Stollberg, J. and Fraser, S.: Acetylcholine Receptors and Con A-Binding Sites on Xenopus Muscle Cells
, J Cell Biol
107,
1397, 1988 | |
| Cells: | Muscle |
| Enzyme(s) | Collagenase: 0.10% |
| Medium | Steinberg's solution |
Muscle: Frog | |
| |
| Shepherd, N. and Kavaler, F.: Direct Control of Contraction Force of Single Frog Atrial Cells by Extracellular Ions
, Am J Physiol 251,
C653, 1986 | |
| Cells: | Myocytes |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | CF Ringer |
Muscle: Frog | |
| |
| Mitra, R. and Morad, M.: A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates
, Am J Physiol 249,
H1056, 1985 | |
| Cells: | Myocytes, heart and stomach |
| Enzyme(s) | Protease XIV: 0.028% |
| Medium | Solution C (see reference) |
Muscle: Frog | |
| |
| Anderson, M.J., Cohen, M.W., and Zorychta, E.: Effects of Innervation on the Distribution of Acetylcholine Receptors on Cultured Muscle Cells
, J Physiol
268,
731, 1977 | |
| Cells: | Muscle |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | L15 medium (see reference) |
Muscle: Guinea-Pig | |
| |
| Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M: Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells
, J Pharmacol Exp Ther 296,
669-75, 2001 | |
| Cells: | Bladder smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.1-0.2% |
| Medium | Krebs-Ringer bicarbonate |
Muscle: Guinea-Pig | |
| |
| Schnitzler, M., Derst, C., Daut, J., and Preisig-Muller, R.: ATP-Sensitive Potassium Channels in Capillaries Isolated From Guinea-Pig Heart
, J Physiol
525 (2),
307, 2000 | |
| Cells: | Capillaries Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | CF solution |
Muscle: Guinea-Pig | |
| |
| Firth, T., Mawe, G., and Nelson, M.: Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle
, Am J Physiol Cell Physiol
278,
C1031, 2000 | |
| Cells: | Smooth muscle Gallbladder |
| Enzyme(s) | Papain: 0.1% |
| Medium | Krebs solution |
Muscle: Guinea-Pig | |
| |
| Jennings, L., Xu, Q., Firth, T., Nelson, M., and Mawe, G.: Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle
, Am J Physiol 277,
G1017, 1999 | |
| Cells: | Smooth muscle Gallbladder |
| Enzyme(s) | Papain: 0.1% |
| Medium | NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES |
Muscle: Guinea-Pig | |
| |
| Ryder, K., Bryant, S., and Hart, G.: Membrane Current Changes in Left Ventricular Myocytes Isolated From Guinea-Pigs After Abdominal Aortic Coarctation
, Cardiovasc Res 27,
1278, 1993 | |
| Cells: | Myocytes |
| Enzyme(s) | Protease: |
| Medium | DMEM |
Muscle: Guinea-Pig | |
| |
| Hu, S., and Kim, H.: Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid, FASEB J
6,
A383, 1992 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Potassium buffer solution |
Muscle: Guinea-Pig | |
| |
| Ohya, Y. and Sperelakis, N.: ATP Regulation of the Slow Calcium Channels in Vascular Smooth Muscle Cells, Circ Res 64,
145, 1989 | |
| Cells: | Smooth muscle Mesenteric artery |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | CF solution |
Muscle: Guinea-Pig | |
| |
| Mitra, R. and Morad, M.: A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates
, Am J Physiol 249,
H1056, 1985 | |
| Cells: | Myocytes, heart and stomach |
| Enzyme(s) | Protease XIV: 0.028% |
| Medium | Solution C (see reference) |
Muscle: Guinea-Pig | |
| |
| Ross, R.: The Smooth Muscle Cell . II. Growth of Smooth Muscle in Culture and Formation of Elastic Fibers, J Cell Biol
50,
172, 1971 | |
| Cells: | Smooth muscle Aortic |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Dulbecco-Vogt modification of Eagle's |
Muscle: Hamster | |
| |
| Nakamura, T., Iwata, Y., Sampaolesi, M., Hanada, H., Saito, N., Artman, M., Coetzee, W., and Shigekawa, M.: Stretch-Activated Cation Channels in Skeletal Muscle Myotubes From Sarcoglycan-Deficient Hamsters, Am J Physiol Cell Physiol
281,
C690, 2001 | |
| Cells: | Satellite |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Muscle: Human | |
| |
| Malysz, J., Rovner, E., Wake, R. and Petkov, G.: Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents.
, J Vis Exp
,
, 2020 | |
| Cells: | Bladder smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.1-0.2% Papain: 0.1-0.2% |
| Medium | Digestion solution (see reference) |
Muscle: Human | |
| |
| Garcia, S., Tamaki, S., Lee, S., Wong, A., Jose, A., Dreux, J., Kouklis, G., Sbitany, H., Seth, R., Knott, P., Heaton, C., Ryan, W., Kim, E., Hansen, S., Hoffman, W. and Pomerantz, J.: High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells.
, Stem Cell Reports
10,
1160-1174, 2018 | |
| Cells: | Muscle satellite |
| Enzyme(s) | Collagenase: 0.1% Trypsin: 0.25% |
| Medium | DMEM |
Muscle: Human | |
| |
| Charville, G., Cheung, T., Yoo, B., Santos, P., Lee, G., Shrager, J. and Rando, T.: Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells.
, Stem Cell Reports
5,
621, 2015 | |
| Cells: | Skeletal muscle stem |
| Enzyme(s) | Collagenase Type 2: 750 u/ml Neutral Protease: 2 u/ml |
| Medium | Ham's F10 |
Muscle: Human | |
| |
| Lu, S., Sun, X., Hong, T., Song, K., Yang, S. and Wang, C.: Isolation and Culture of Smooth Muscle Cells from Human Acute Type A Aortic Dissection.
, J Cardiothorac Surg
8,
83, 2013 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Muscle: Human | |
| |
| Stadler, G., Chen, J., Wagner, K., Robin, J., Shay, J., Emerson, C. and Wright, W.: Establishment of Clonal Myogenic Cell Lines from Severely Affected Dystrophic Muscles - CDK4 Maintains the Myogenic Population.
, Skelet Muscle
1,
12, 2011 | |
| Cells: | Myogenic |
| Enzyme(s) | Collagenase Type 4: 0.1% Neutral Protease: 2.4 u/ml |
| Medium | HBSS |
Muscle: Human | |
| |
| Nesti, L., Jackson, W., Shanti, R., Koehler, S., Aragon, A., Bailey, J., Sracic, M., Freedman, B., Giuliani, J. and Tuan, R.: Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue.
, J Bone Joint Surg Am
90,
2390, 2008 | |
| Cells: | Muscle derived multiprogenitor cells |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | DMEM |
Muscle: Human | |
| |
| Moss, S., Bates, M., Parrino, P. and Woods, TC.: Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue.
, Ochsner J
7,
133, 2007 | |
| Cells: | Endothelial and vascular smooth muscle |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Muscle: Human | |
| |
| Kimuli, M., Eardley, I. and Southgate, J.: In Vitro Assessment of Decellularized Porcine Dermis as a Matrix for Urinary Tract Reconstruction.
, BJU Int 94,
859, 2004 | |
| Cells: | Urinary tract smooth muscle |
| Enzyme(s) | Collagenase Type 4: 100 u/ml |
| Medium | DMEM |
Muscle: Human | |
| |
| Richardson, M., Taylor, D., Casey, M., MacDonald, P., and Stull, J.: Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture, In Vitro Cell Dev Biol 23,
21, 1987 | |
| Cells: | Smooth muscle Myometrial |
| Enzyme(s) | Deoxyribonuclease I: 0.015% and 0.007% |
| Medium | HBSS |
Muscle: Human | |
| |
| Casey, M., MacDonald, P., Mitchell, M., and Snyder, J.: Maintenance and Characterization of Human Myometrial Smooth Muscle Cells in Monolayer Culture, In Vitro 20,
396, 1984 | |
| Cells: | Smooth muscle Myometrial |
| Enzyme(s) | Deoxyribonuclease I: 0.12% |
| Medium | HBSS |
Muscle: Human | |
| |
| Davies, P. and Kerr, C.: Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts
, Biochim Biophys Acta 712,
26, 1982 | |
| Cells: | Smooth muscle, fibroblasts |
| Enzyme(s) | Trypsin: 0.055% |
| Medium | DMEM |
Muscle: Human | |
| |
| Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8),
713, 1981 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Muscle: Human | |
| |
| Chamley-Campbell, J., Campbell, G., and Ross, R.: The Smooth Muscle in Cell Culture
, Physiol Res 59,
1, 1979 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Trypsin: 0.05% - 0.1% |
| Medium | HBSS |
Muscle: Lizard | |
| |
| Cox, P., and Simpson, Jr., S.: A Microphotometric STudy of Myogenic Lizard Cells Grown in Vitro
, Dev Biol 23,
433, 1970 | |
| Cells: | Myoblasts, tail |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | GM III (see reference) |
Muscle: Monkey | |
| |
| Chait, A., Ross, R., Albers, J., and Bierman, E.: Platelet-Derived Growth Factor Stimulates Activity of LDL Receptors, Proc Natl Acad Sci U S A 77,
4084, 1980 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Dulbecco-Vogt |
Muscle: Monkey | |
| |
| Chamley, J., Campbell, G., McConnell, J., and Groschel-Stewart, U.: Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture
, Cell Tissue Res 177,
503, 1977 | |
| Cells: | Smooth muscle, saphenous vein |
| Enzyme(s) | Elastase: 0.05% |
| Medium | BSS |
Muscle: Mouse | |
| Huttner,S., Ahrens, H., Schmidt, M., Henze, H., Jung, M., Schuler. S. and von Maltzahn, J.: Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle.
, Methods Mol Biol ,
, 2019 | |
| Cells: | Myofiber |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM |
Muscle: Mouse | |
| |
| Eckhardt, J., Bachmann, C., Sekulic-Jablanovic, M., Enzmann, V., Park, K., Ma, J., Takeshima, H., Zorzato, F. and Treves, S.: Extraocular Muscle Function is Impaired in ryr3−/− Mice
, J Gen Physiol 151,
929-943, 2019 | |
| Cells: | Extraocular muscles |
| Enzyme(s) | Collagenase Type 1: 0.1% Collagenase Type 2: 0.03-0.2% Elastase: 0.0125-0.1% |
| Medium | Ringer |
Muscle: Mouse | |
| |
| Sachdeva, J., Mahajan, A., Cheng, J., Baeten, J., Lilly, B., Kuivaniemi, H. and Hans, C.: Smooth Muscle Cell-Specific Notch1 Haploinsufficiency Restricts the Progression of Abdominal Aortic Aneurysm by Modulating CTGF Expression.
, PLoS ONE
12,
e0178538, 2017 | |
| Cells: | vascular smooth muscle |
| Enzyme(s) | Collagenase Type 1: 0.1% Elastase: 0.75 u/ml |
| Medium | DMEM/F12 |
Muscle: Mouse | |
| |
| Birbrair, A, Sattiraju, A, Zhu, D, Zulato, G, Batista, I, Nguyen, V, Messi M, Solingapuram Sai, K, Marini, F, Delbono, O and Mintz, A.: Novel Peripherally Derived Neural-Like Stem Cells as Therapeutic Carriers for Treating Glioblastomas.
, Stem Cells Transl Med ,
, 2016 | |
| Cells: | Muscle derived NLSC |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Muscle: Mouse | |
| |
| Randolph, M., Phillips, B., Choo, H., Vest, K., Vera, Y. and Pavlath, G.: Pharyngeal Satellite Cells Undergo Myogenesis Under Basal Conditions and Are Required for Pharyngeal Muscle Maintenance.
, Stem Cells 33,
3581-95, 2015 | |
| Cells: | Pharyngeal myofiber |
| Enzyme(s) | Collagenase Type 1: 400-800 u/ml |
| Medium | DMEM |
Muscle: Mouse | |
| |
| Abou-Khalil, R., Yang, F., Lieu, S., Julien, A., Perry, J., Pereira, C., Relaix, F., Miclau, T., Marcucio, R. and Colnot, C.: Role of Muscle Stem Cells During Skeletal Regeneration.
, Stem Cells 33,
1501-11, 2015 | |
| Cells: | Myoblasts |
| Enzyme(s) | Collagenase Type 2: 400 u/ml Neutral Protease: 0.5 u/ml |
| Medium | Hams F-10 |
Muscle: Mouse | |
| |
| Pasut, A., Jones, A. and Rudnicki, M.: Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle.
, J Vis Exp
73,
e50074, 2013 | |
| Cells: | Myofibers |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM |
Muscle: Mouse | |
| |
| Metz, R., Patterson, J. and Wilson, E.: Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization.
, Methods Mol Biol 843,
169, 2012 | |
| Cells: | Vascular smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.14% |
| Medium | Basal Culture |
Muscle: Mouse | |
| |
| Rezk, B., Yoshida, T., Semprun-Prieto, L., Higashi, Y., Sukhanov, S. and Delafontaine, P.: Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy.
, PLoS ONE
7,
e30276, 2012 | |
| Cells: | Diaphragm cells |
| Enzyme(s) | Collagenase Type 2: 100 u/ml Pronase: 0.125% |
| Medium | PBS |
Muscle: Mouse | |
| |
| Weisleder, N., Zhou, J. and Ma, J.: Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers.
, Methods Mol Biol 798,
395, 2012 | |
| Cells: | Skeletal muscle fiber |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | Tyrode |
Muscle: Mouse | |
| |
| Shi, H., Boadu, E., Mercan, F., Le, A., Roth Flach, R., Zhang, L., Tyner, K., Olwin, B. and Bennett, A.: MAP Kinase Phosphatase-1 Deficiency Impairs Skeletal Muscle Regeneration and Exacerbates Muscular Dystrophy.
, FASEB J
24,
2985, 2010 | |
| Cells: | Myoblast |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Muscle: Mouse | |
| |
| Ieronimakis Nicholas, Balasundaram Gayathri, Reyes Morayma: Direct isolation, culture and transplant of mouse skeletal muscle derived endothelial cells with angiogenic potential
, PLoS ONE
3,
e0001753, 2008 | |
| Cells: | Myocytes, endothelial |
| Enzyme(s) | Neutral Protease: 1.2 u/ml Collagenase Type 4: 0.2% |
| Medium | PBS |
Muscle: Mouse | |
| |
| Li CX, Liu BH, Tong WD, Zhang LY, and Jiang YP: Dissociation, culture and morphologic changes of interstitial cells of Cajal in vitro
, World J Gastroenterol
11,
2838, 2005 | |
| Cells: | Intersitial cells of Cajal |
| Enzyme(s) | Collagenase Type 2: 0.13% |
| Medium | M199 |
Muscle: Mouse | |
| |
| Johnson, B., Scheuer, T. and Catterall, W.: Convergent Regulation of Skeletal Muscle Ca2+ Channels by Dystrophin, the Actin Cytoskeleton, and cAMP-Dependent Protein Kinase.
, Proc Natl Acad Sci U S A 102,
4191, 2005 | |
| Cells: | Skeletal muscle myotubes |
| Enzyme(s) | NCIS kit: per instructions |
| Medium | L-15 |
Muscle: Mouse | |
| |
| Winitsky, S., Gopal, T., Hassanzadeh, S., Takahashi, H., Gryder, D., Rogawski, M., Takeda, K., Yu, Z., Xu, Y. and Epstein, N.: Adult Murine Skeletal Muscle Contains Cells That Can Differentiate into Beating Cardiomyocytes In Vitro.
, PLoS Biol
3,
e87, 2005 | |
| Cells: | Precursor cells |
| Enzyme(s) | Collagenase Type 2: 0.5% |
| Medium | DMEM/F12 |
Muscle: Mouse | |
| |
| Majka S., Jackson K., Kienstra K., Majesky M., Goodell M., and Hirschi K.: Distinct Progenitor Populations in Skeletal Muscle Are Bone Marrow Derived and Exhibit Different Cell Fates During Vascular Regeneration
, J Clin Invest 111,
71, 2003 | |
| Cells: | Skeletal muscle progenitor |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Muscle: Mouse | |
| |
| Fukada S., Miyagoe-Suzuki Y., Tsukihara H., Yuasa K., Higuchi S., Ono S., Tsujikawa K., Takeda S., and Yamamoto H.: Muscle Regeneration by Reconstitution with Bone Marrow or Fetal Liver Cells from Green Fluorescent Protein-gene Transgenic Mice
, J Cell Sci
115,
1285, 2002 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 1: 0.5% |
| Medium | DMEM |
Muscle: Mouse | |
| |
| McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.: Muscle-derived hematopoietic stem cells are hematopoietic in origin
, Proc Natl Acad Sci U S A 99,
1341, 2002 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% Trypsin: 0.25% |
| Medium | HBSS |
Muscle: Ovine | |
| |
| Driska S., Laudadio R., Wolfson M., and Shaffer T.: A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity
, J Appl Physiol 86(1),
427, 1999 | |
| Cells: | Tracheal smooth muscle cells |
| Enzyme(s) | Papain: 0.2% Deoxyribonuclease I: 0.1% |
| Medium | MOPS-PSS |
Muscle: Porcine | |
| |
| Miersch, C., Stange, K. and Rontgen, M.: Effects of Trypsinization and of a Combined Trypsin, Collagenase, and DNase Digestion on Liberation and in Vitro Function of Satellite Cells Isolated from Juvenile Porcine Muscles.
, In Vitro Cell Dev Biol Anim 54,
406-412, 2018 | |
| Cells: | Muscle satellite |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 0.2% Deoxyribonuclease I: 0.01% |
| Medium | Eagle MEM |
Muscle: Porcine | |
| |
| Lewis, F., Henning, B., Marazzi, G., Sassoon, D., Ellison, G. and Nadal-Ginard, B.: Porcine Skeletal Muscle-Derived Multipotent PW1pos/Pax7neg Interstitial Cells: Isolation, Characterization, and Long-Term Culture.
, Stem Cells Transl Med 3,
702-12, 2014 | |
| Cells: | Skeletal muscle |
| Enzyme(s) | Collagenase: 10% Neutral Protease: 0.3% |
| Medium | HBSS |
Muscle: Porcine | |
| |
| Korzick, D., Laughlin, M., and Bowles, D.: Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries
, J Appl Physiol 96,
1425-32, 2004 | |
| Cells: | Coronary myocytes |
| Enzyme(s) | Collagenase Type 2: 294 u/ml Elastase: 6.5 u/ml Deoxyribonuclease I: 0.04% Soybean Trypsin Inhibitor: 0.1% |
| Medium | low calcium physiological saline |
Muscle: Porcine | |
| |
| Wamhoff BR, Dixon JL, Sturek M: Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia
, J Vasc Res 39,
208, 2002 | |
| Cells: | Arterial smooth muscle |
| Enzyme(s) | Collagenase Type 2: 294 u/ml Elastase: 6.5 u/ml Deoxyribonuclease I: 0.4 mg/ml Soybean Trypsin Inhibitor: 1 mg/ml |
| Medium | MEM |
Muscle: Porcine | |
| |
| Sirous ZN, Fleming JB, Khalil RA: Endothelin-1 enhances eicosanoids-induced coronary smooth muscle contraction by activating specific protein kinase C isoforms
, Hypertension 37,
497-504, 2001 | |
| Cells: | Coronary smooth muscle |
| Enzyme(s) | Collagenase Type 2: 150 u/ml Elastase: 0.05% |
| Medium | HBSS |
Muscle: Porcine | |
| |
| Shieh CC, Feng J, Buckner SA, Brioni JD, Coghlan MJ, Sullivan JP, Gopalakrishnan M: Functional implication of spare ATP-sensitive K(+) channels in bladder smooth muscle cells
, J Pharmacol Exp Ther 296,
669-75, 2001 | |
| Cells: | Bladder smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.1-0.2% |
| Medium | Krebs-Ringer bicarbonate |
Muscle: Porcine | |
| |
| Huckle WR, Drag MD, Acker WR, Powers M, McFall RC, Holder DJ, Fujita T, Stabilito II, Kim D, Ondeyka DL, Mantlo NB, Chang RS, Reilly CF, Schwartz RS, Greenlee WJ, Johnson RG: Effects of subtype-selective and balanced angiotensin II receptor antagonists in a porcine coronary artery model of vascular restenosis
, Circulation 93,
1009-19, 1996 | |
| Cells: | Coronary smooth muscle cells |
| Enzyme(s) | Collagenase: 0.3% Elastase: 0.05% |
| Medium | HBSS |
Muscle: Porcine | |
| |
| Xiong, Yimin, Xu, Shangzhe, and Slakey, Linda L: Modulation of Response to Adenosine in Vascular Smooth Muscle Cells Cultured in Defined Medium, In Vitro Cell Dev Biol 27,
355, 1991 | |
| Cells: | Smooth muscle, aorta |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | DMEM |
Muscle: Porcine | |
| |
| Breton, M., Berrou, E., Deudon, E. and Picard, J.: Changes in Proteoglycans of Cultured Pig Aortic Smooth Muscle Cells During Subculture, In Vitro Cell Dev Biol 26,
157, 1990 | |
| Cells: | Smooth muscle Aorta |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | EDTA 0.02% |
Muscle: Porcine | |
| |
| Fehr, T., Dickinson, E., Goldman, S. and Slakey, L.: Cyclic AMP Efflux is Regulated by Occupancy of The Adenosine Receptor in Pig Aortic Smooth Muscle Cells, J Biol Chem 265,
10974, 1990 | |
| Cells: | Smooth muscle Aortic medial tissue |
| Enzyme(s) | Collagenase: 0.30% |
| Medium | DMEM |
Muscle: Quail | |
| |
| Konigsberg, I.: Skeletal Myoblasts in Culture, Methods Enzymol
LVIII,
511, 1979 | |
| Cells: | Myoblasts |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | Puck's solution |
Muscle: Rabbit | |
| |
| Al-Shboul, O., Mahavadi, S., Sriwai, W., Grider, J. and Murthy, K.: Differential Expression of Multidrug Resistance Protein 5 and Phosphodiesterase 5 and Regulation of cGMP Levels in Phasic and Tonic Smooth Muscle.
, Am J Physiol Gastrointest Liver Physiol
305,
G314-24, 2013 | |
| Cells: | Gastric smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.1% Soybean Trypsin Inhibitor: 0.01% |
| Medium | DMEM |
Muscle: Rabbit | |
| |
| Croons Valerie, Martinet Wim, Herman Arnold G, Timmermans Jean-Pierre, De Meyer Guido R Y: Selective clearance of macrophages in atherosclerotic plaques by the protein synthesis inhibitor cycloheximide
, J Pharmacol Exp Ther 320,
986-93, 2007 | |
| Cells: | Aortic smooth muscle |
| Enzyme(s) | Collagenase Type 2: 300 u/ml Elastase: 5 u/ml |
| Medium | F10 Ham's |
Muscle: Rabbit | |
| |
| Santos, M., Nguyen, B.., Thompson, J.: Factors Affecting in Vitro Growth of Harvested Enterocytes
, Cell Transplant 1,
299, 1992 | |
| Cells: | Enterocytes |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | RPMI 1640 w/ 1% fetal bovine serum PBS |
Muscle: Rabbit | |
| |
| Hu, S., and Kim, H.: Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid, FASEB J
6,
A383, 1992 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Potassium buffer solution |
Muscle: Rabbit | |
| |
| Benham, C., Bolton, T., Byrne, N., and Large, W.: Action of Extremely Applied Adenosine Triphosphate O Single Smooth Muscle Cells Dispersed From Rabbit Ear Artery
, J Physiol
387,
473, 1987 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Elastase: 0.17 - 0.25% |
| Medium | Saline |
Muscle: Rabbit | |
| |
| Knodle, S., Anderson, S., and Papaioannou, S.: Large Scale Preparation of Rabbit Aortic Smooth Muscle Cells For Use in Calcium Uptake Studies, In Vitro Cell Dev Biol 22,
23, 1986 | |
| Cells: | Smooth muscle, aortic |
| Enzyme(s) | Trypsin: 0.038% |
| Medium | MEM |
Muscle: Rabbit | |
| |
| Benham, C.D., Bolton, T.B.: Spontaneous Transient Outward Currents in Single Visceral and Vascular Smooth Muscle Cells of the Rabbit
, J Physiol
381,
385, 1986 | |
| Cells: | Smooth muscle, ear artery |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | CF solution (see reference) |
Muscle: Rabbit | |
| |
| Mitra, R. and Morad, M.: A Uniform Enzymatic Method for Dissociation of Myocytes from Hearts and Stomachs of Vertebrates
, Am J Physiol 249,
H1056, 1985 | |
| Cells: | Myocytes, heart and stomach |
| Enzyme(s) | Protease XIV: 0.028% |
| Medium | Solution C (see reference) |
Muscle: Rabbit | |
| |
| Ives, H., Schultz, G., Galardy, R., and Jamieson, J.: Preparation of Functional Smooth Muscle Cells from the Rabbit Aorta, J Exp Med 148,
1400, 1978 | |
| Cells: | Smooth muscle, aorta |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Krebs Ringer HEPES solution |
Muscle: Rabbit | |
| |
| Chamley, J., Campbell, G., McConnell, J., and Groschel-Stewart, U.: Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture
, Cell Tissue Res 177,
503, 1977 | |
| Cells: | Smooth muscle, saphenous vein |
| Enzyme(s) | Elastase: 0.05% |
| Medium | BSS |
Muscle: Rabbit | |
| |
| Peters, T., Muller, M., and deDuve, C.: Lysosomes of the Arterial Wall. I. Isolation and Subcellular Fractionation of Cells from Normal Rabbit Aorta, J Exp Med 136,
1117, 1972 | |
| Cells: | Smooth muscle, aorta |
| Enzyme(s) | Hyaluronidase: 800 u/ml |
| Medium | HBSS |
Muscle: Rabbit | |
| |
| Day, A., Phil, D., and Newman, H.: Synthesis of Phospholipid by Foam Cells Isolated from Rabbit Atherosclerotic Lesions, Circ Res XIX,
122, 1966 | |
| Cells: | Thoracic aorta |
| Enzyme(s) | Elastase: 0.008% |
| Medium | Kreb's Ringer |
Muscle: Rat | |
| |
| Vilmont, V., Cadot, B., Ouanoumou, G. and Gomes, E.: A System for Studying Mechanisms of Neuromuscular Junction Development and Maintence
, Development
143,
2464, 2016 | |
| Cells: | Skeletal muscle |
| Enzyme(s) | Collagenase: 0.05% Neutral Protease: 0.35% |
| Medium | DMEM |
Muscle: Rat | |
| |
| Zeng, Y., Liu, H., Kang, K., Wang, Z., Hui, G., Zhang, X., Zhong, J., Peng, W., Ramchandran, R., Raj, J. and Gou, D.: Hypoxia Inducible Factor-1 Mediates Expression of miR-322: Potential Role in Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells.
, Sci Rep
5,
12098, 2015 | |
| Cells: | Pulmonary arterial smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.1% Elastase: 0.05% |
| Medium | Buffer |
Muscle: Rat | |
| |
| Weisleder, N., Zhou, J. and Ma, J.: Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers.
, Methods Mol Biol 798,
395, 2012 | |
| Cells: | Skeletal muscle fiber |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | Tyrode |
Muscle: Rat | |
| |
| Weber, S., Gratopp, A., Akanbi, S., Rheinlaender, C., Sallmon, H., Barikbin, P. and Koehne, P.: Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus
, Pediatr Res 70,
236, 2011 | |
| Cells: | Vascular smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.1% Elastase: 0.02% Soybean Trypsin Inhibitor: 0.05% Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Muscle: Rat | |
| |
| Das, M., Rumsey, J., Bhargava, N., Stancescu, M. and Hickman, J.: Skeletal Muscle Tissue Engineering: A Maturation Model Promoting Long-Term Survival of Myotubes, Structural Development of the Excitation-Contraction Coupling Apparatus and Neonatal Myosin Heavy Chain Expression.
, Biomaterials 30,
5392, 2009 | |
| Cells: | Myotubes |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | see reference |
Muscle: Rat | |
| |
| Pacak, C. and Cowan, D.: Fabrication of Myogenic Engineered Tissue Constructs.
, J Vis Exp
27,
e1137, 2009 | |
| Cells: | Myogenic |
| Enzyme(s) | Collagenase Type 2: 1.0% Neutral Protease: 2.4 u/ml |
| Medium | Ham's F-10 |
Muscle: Rat | |
| |
| Larkin, L., Calve, S., Kostrominova, T. and Arruda, E.: Structure and Functional Evaluation of Tendon-Skeletal Muscle Constructs Engineered In Vitro.
, Tiss Eng
12,
3149-58, 2006 | |
| Cells: | myogenic |
| Enzyme(s) | Neutral Protease: 8 u/ml Collagenase Type 4: 200 u/ml |
| Medium | DMEM |
Muscle: Rat | |
| |
| Kim, J., Hadlock, T., Cheney, M., Varvares, M. and Marler, J.: Muscle Tissue Engineering for Partial Glossectomy Defects.
, Arch Facial Plast Surg 5,
403, 2003 | |
| Cells: | Myoblasts |
| Enzyme(s) | Collagenase Type 2: 1.0% Neutral Protease: 2.4 u/ml |
| Medium | Ham's F-10 |
Muscle: Rat | |
| |
| Dennis, R., Kosnik II, P., Gilbert, M., and Faulkner, J.: Excitability and Contractility of Skeletal Muscle Engineered from Primary Cultures and Cell Lines, Am J Physiol Cell Physiol
280,
C288, 2001 | |
| Cells: | Myooids |
| Enzyme(s) | Neutral Protease: 4 u/ml |
| Medium | Ham's F-12 |
Muscle: Rat | |
| |
| Su E., Stevenson S., Rollence M., Marshall-Neff J., and Liau G.: A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells
, J Vasc Res 38(5),
471, 2001 | |
| Cells: | Smooth muscle cells |
| Enzyme(s) | Collagenase Type 2: 0.2% Elastase: 0.04% Soybean Trypsin Inhibitor: 0.1% |
| Medium | M-199 |
Muscle: Rat | |
| |
| Jaggar JH: Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells
, Am J Physiol Cell Physiol
281,
C439-48, 2001 | |
| Cells: | Arterial smooth muscle |
| Enzyme(s) | Papain: 0.03% Collagenase: 0.1% |
| Medium | see reference |
Muscle: Rat | |
| |
| Wellman, G., Barrett-Jolley, R., Koppel, H. Everitt, D., and Quayle, J.: Inhibition of Vascular KATP Channels by U-37883A: A Comparison with Cardiac and Skeletal Muscle, Br J Pharmacol 128,
909, 1999 | |
| Cells: | Smooth & skeletal muscle Cardiac myocytes |
| Enzyme(s) | Protease: 0.01% |
| Medium | PSS |
Muscle: Rat | |
| |
| Hrometz, S., Edelmann, S., McCune, D., Olges, J., Hadley, R., Perez ,D., and Piascik, M.: Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction
, J Pharmacol Exp Ther 290(1),
452, 1999 | |
| Cells: | Vascular smooth muscle |
| Enzyme(s) | Collagenase Type 2: 0.1% Elastase: 0.0125% |
| Medium | DMEM |
Muscle: Rat | |
| |
| Redmond, E., Cahill, P., and Sitzmann, J.: Perfused Transcapillary Smooth Muscle and Endothelial Cell Co-Culture-A Novel In Vitro Model, In Vitro Cell Dev Biol Anim 31,
601, 1995 | |
| Cells: | Smooth muscle, endothelial |
| Enzyme(s) | Trypsin: 0.04% |
| Medium | MEM |
Muscle: Rat | |
| |
| McGuire, P., Walker-Caprioglio, H., Little, S., and McGuffee, L.: Isolation and Culture of Rat Superior Mesenteric Artery Smooth Muscle Cells, In Vitro Cell Dev Biol 29,
135, 1993 | |
| Cells: | Smooth muscle, mesenteric artery |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | MEM |
Muscle: Rat | |
| |
| Hu, S., and Kim, H.: Activation of K+ Channel in Vascular Smooth Muscle by Cytochrome P-450 Metabolites of Arachidonic Acid, FASEB J
6,
A383, 1992 | |
| Cells: | Smooth muscle |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Potassium buffer solution |
Muscle: Rat | |
| |
| Loch-Caruso, R., Pahl, M., and Juberg, D.: Rat Myometrial Smooth Muscle Cells Show High Levels of Gap Junctional Communication Under a Variety of Culture Conditions
, In Vitro Cell Dev Biol 28,
97, 1992 | |
| Cells: | Smooth muscle, myometrial |
| Enzyme(s) | Trypsin: 150 µg/ml |
| Medium | HBSS or PSS, CMF |
Muscle: Rat | |
| |
| Bolzon, B. and Cheung, D.: Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats
, Hypertension 14,
137, 1989 | |
| Cells: | Smooth muscle, tail arteries |
| Enzyme(s) | Papain: 0.1% |
| Medium | HEPES buffer (see reference) |
Muscle: Rat | |
| |
| Barone, L., Wolfe, L., Faris, B., and Franzblau, C.: Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat
, Biochemistry
27,
3175, 1988 | |
| Cells: | Smooth muscle, aortic |
| Enzyme(s) | Elastase: 0.0125% |
| Medium | DMEM |
Muscle: Rat | |
| |
| Bean, B., Sturek, M., Puga, A., and Hermsmeyer, K.: Calcium Channels in Muscle Cells Isolated From Rat Mesenteric Arteries: Modulation by Dihydropyridine Drugs
, Circ Res 59,
229, 1986 | |
| Cells: | Muscle, mesenteric arteries |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HEPES KG solution (see reference) |
Muscle: Rat | |
| |
| Gordon, D., Mohai, L., and Schwartz, S.: Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells
, Circ Res 59,
633, 1986 | |
| Cells: | Endothelial, aortic |
| Enzyme(s) | Elastase: 0.05% |
| Medium | Waymouth's culture medium |
Muscle: Rat | |
| |
| Cole, O., Fan, T., and Lewis, G.: Isolation, Characterization, Growth and Culture of Endothelial Cells From the Rat Aorta
, Cell Biol Int Rep 10 (6),
399, 1986 | |
| Cells: | Endothelial, aortic |
| Enzyme(s) | Trypsin: |
| Medium | RPMI 1640 |
Muscle: Rat | |
| |
| Boulanger-Saunier, C., Kattenburg, D., and Stoclet, J.: Cyclic AMP-dependent Phosphorylation of a 16kDa Protein in a Plasma Membrane-enriched Fraction of Rat Aortic Myocytes
, F.E.B.S. Lett. 193,
283, 1985 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HBSS |
Muscle: Rat | |
| |
| Brock, T., Alexander, R., Ekstein, L., Atkinson, W., and Gimbrone, M.: Angiotensin Increases Cytosolic Free Calcium in Cultured Vascular Smooth Muscle Cells
, Hypertension 7,
105, 1985 | |
| Cells: | Smooth muscle, thoracic aorta |
| Enzyme(s) | Trypsin: 0.0375% |
| Medium | Eagle's MEM with calcium |
Muscle: Rat | |
| |
| Gunther S, Alexander RW, Atkinson WJ, and Gimbrone MA Jr.: Functional angiotensin II receptors in cultured vascular smooth muscle cells
, J Cell Biol
92,
289, 1982 | |
| Cells: | Mesenteric artery smooth muscle cells |
| Enzyme(s) | Elastase: .0125% Soybean Trypsin Inhibitor: 0.025% Collagenase Type 1: 0.1% |
| Medium | HBSS |
Muscle: Rat | |
| |
| Kasten, F.: Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties
, In Vitro 8,
128, 1972 | |
| Cells: | Myocardial |
| Enzyme(s) | Trypsin NF 1:250: 0.125% |
| Medium | HBSS CMF |
Muscle: Rat | |
| |
| Kono, T.: Roles of Collagenases and Other Proteolytic Enzymes in the Dispersal of Animal Tissues
, Biochim Biophys Acta 178,
397, 1969 | |
| Cells: | Muscle |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Kreb's Henseleit bicarbonate buffer |
Muscle: Rat | |
| |
| Harary, I., and Farley, B.: In Vitro Studies on Single Beating Rat Heart Cells
, Exp Cell Res
29,
451, 1963 | |
| Cells: | Heart |
| Enzyme(s) | Trypsin NF 1:250: 250: 0.1% |
| Medium | Saline A (See reference) |
Neural: Avian | |
| |
| Rollo, B., Zhang, D., Simkin, J., Menheniott, T. and Newgreen, D.: Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells.
, F1000Res
4,
113, 2015 | |
| Cells: | Midgut DRG |
| Enzyme(s) | Collagenase animal free: 0.05% Neutral Protease: 0.5% |
| Medium | Ham's F12 |
Neural: Avian | |
| |
| Temburni, M., Rosenberg, M., Pathak, N., McConnell, R. and Jacob, M.: Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein.
, J Neurosci 24,
6776, 2004 | |
| Cells: | Cilary ganglion neurons |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Neural: Bovine | |
| |
| Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons
, In Vitro Cell Dev Biol 26,
335, 1990 | |
| Cells: | Heart Adrenal chromaffin Paraneurons |
| Enzyme(s) | Trypsin: 0.06% |
| Medium | 25mM HEPES buffered Locke's solution, CMF |
Neural: Bovine | |
| |
| Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina
, In Vitro 18 (7),
626, 1982 | |
| Cells: | Microvascular endothelial |
| Enzyme(s) | Collagenase/Dispase: 0.1% |
| Medium | MEM |
Neural: Bovine | |
| |
| Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain
, J Biol Chem 253,
1592, 1978 | |
| Cells: | Oligodendroglia Neural |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | (see reference) |
Neural: Canine | |
| |
| Tongtako, W., Lehmbecker, A., Wang, Y., Hahn, K., Baumgartner, W. and Gerhauser, I.: Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties.
, Sci Rep
7,
13915, 2017 | |
| Cells: | Satellite glial |
| Enzyme(s) | Hyaluronidase: 0.2% Collagenase Type 4: 0.2% Collagenase: 0.2% |
| Medium | HBSS |
Neural: Chicken | |
| |
| Watt, P., Mullen, Y., Benhamou, P., Hober, C., Nomura, Y., Watanabe, Y., Passaro, E., Zinner, M., and Brunicardi, F.: Simplified Semiautomated Method For Isolating Islets From the Human Pancreas
, Transplant Proc 26 (2),
582, 1994 | |
| Cells: | Islets |
| Enzyme(s) | Trypsin: Trypsin: 0.125-0.25% Collagenase: 0.2% Deoxyribonuclease I: 200 u/ml |
| Medium | Eurocollins solution |
Neural: Chicken | |
| |
| Raman, I., and Trussell, L.: The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus
, Neuron 9,
173, 1992 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 40 u/ml |
| Medium | HEPES |
Neural: Chicken | |
| |
| Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina
, In Vitro Cell Dev Biol 26,
1073, 1990 | |
| Cells: | Flat, retina |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Tyrode's solution, CMF |
Neural: Chicken | |
| |
| Schnaar, R., and Schaffner, A.: Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions
, J Neurosci 1,
204, 1981 | |
| Cells: | Spinal cord |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Phosphate buffer (see reference) |
Neural: Chicken | |
| |
| Choi, D., and Fiscbach, G.: GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture
, J Neurophysiol 45,
605, 1981 | |
| Cells: | Dorsal root ganglion neurons Spinal cord |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Puck's saline, CMF |
Neural: Chicken | |
| |
| Bottenstein, J., Skaper, S., Varon, S., and Sato, G.: Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium
, Exp Cell Res
125,
183, 1980 | |
| Cells: | Neurons, ganglia |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS, CMF |
Neural: Chicken | |
| |
| Tuttle, J., Suszkiw, J., and Ard, M.: Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture
, Brain Res 183,
161, 1980 | |
| Cells: | Ciliary ganglion neurons |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Eagle's MEM |
Neural: Chicken | |
| |
| Mudge, A., Leeman, S., and Fischbach, G.: Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration
, Proc Natl Acad Sci U S A 76 (1),
526, 1979 | |
| Cells: | Dorsal root ganglia neurons |
| Enzyme(s) | Collagenase: 0.01% |
| Medium | Eagle's MEM |
Neural: Chicken | |
| |
| McCarthy, K., and Partlow, L.: Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates
, Brain Res 114,
391, 1976 | |
| Cells: | Ganglion chains, sympathetic ganglia |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Krebs Phosphosaline |
Neural: Chicken | |
| |
| Wiseman, L., and Hammond, W.: The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents
, J Exp Zool 197,
429, 1976 | |
| Cells: | Neural retina |
| Enzyme(s) | Collagenase: 0.25% Elastase: 0.2% Hyaluronidase: 1.0% Papain: 1.0% Protease: 0.1% Trypsin: 0.05% |
| Medium | HBSS |
Neural: Fish | |
| |
| Spiro, Z., Koh, A., Tay, S., See, K. and Winkler, C.: Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish.
, Sci Rep
6,
27470, 2016 | |
| Cells: | Motorneurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | L-15 |
Neural: Fish | |
| |
| Sakowski, S., Lunn, J., Busta, A., Palmer, M., Dowling, J. and Feldman, E.: A Novel Approach to Study Motor Neurons from Zebrafish Embryos and Larvae in Culture.
, J Neurosci Methods 205,
277-82, 2012 | |
| Cells: | Motor neurons |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | See reference |
Neural: Fish | |
| |
| Won, Y., Ono, F. and Ikeda, S.: Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons.
, J Neurophysiol 105,
442-53, 2011 | |
| Cells: | Rohon-Beard neurons |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | L-15/Hepes |
Neural: Fish | |
| |
| Cerda, G., Hargrave, M. and Lewis, K.: RNA Profiling of FAC-sorted Neurons from the Developing Zebrafish Spinal Cord.
, Dev Dyn
238,
150-61, 2009 | |
| Cells: | Neurons |
| Enzyme(s) | PDS kit: with modifications |
| Medium | EBSS |
Neural: Fish | |
| |
| Anderson, M.J.: Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro
, In Vitro Cell Dev Biol 29A,
145, 1993 | |
| Cells: | Neurons, spinal cord |
| Enzyme(s) | Trypsin: 0.4% |
| Medium | PBS, CMF |
Neural: Frog | |
| |
| Takahashi, T., Nakajima, Y., Hirosawa, K., Nakajima, S., and Onodera, K.: Structure and Physiology of Developing Neuromuscular Synapses in Culture
, J Neurosci 7,
473, 1987 | |
| Cells: | Neuron |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | Steinberg's solution |
Neural: Guinea-Pig | |
| |
| Jessen, K, McConnell, J., Purves, R., Burnstock, G., and Chamley-Campbell, J.: Tissue Culture of Mammalian Enteric Neurons
, Brain Res 152,
573, 1978 | |
| Cells: | Neuron, enteric |
| Enzyme(s) | Trypsin: 0.125% |
| Medium | Medium 199 |
Neural: Hamster | |
| |
| Liman ER: Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons
, J Physiol
548,
777, 2003 | |
| Cells: | Vomeronasal organ neurons |
| Enzyme(s) | Collagenase Type 1: 0.02% Trypsin: 0.02% |
| Medium | PBS |
Neural: Human | |
| |
| Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord.
, J Vis Exp
,
e52732, 2015 | |
| Cells: | Spinal cord neural progenitor |
| Enzyme(s) | PDS kit: with modifications |
| Medium | EBSS |
Neural: Human | |
| |
| Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24
, Stem Cells 25,
1560-70, 2007 | |
| Cells: | Neural, various |
| Enzyme(s) | Papain: 12 u/ml Trypsin: see reference Collagenase/Dispase: see reference |
| Medium | see reference |
Neural: Human | |
| |
| Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.: EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy
, Nat Med 11,
277, 2005 | |
| Cells: | Neurons |
| Enzyme(s) | Collagenase Type 4: 1.33% Papain: 0.07 u/ml Neutral Protease: 1 mg/ml |
| Medium | DMEM/F12 |
Neural: Human | |
| |
| Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.: Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells
, J Neurosci Res 75,
782, 2004 | |
| Cells: | Neural progenitor cells |
| Enzyme(s) | Papain: 2.5 u/ml Deoxyribonuclease I: 250 u/ml Neutral Protease: 1 u/ml |
| Medium | DMEM/F-12 |
Neural: Human | |
| |
| Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA: Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone
, J Neurosci Res 59,
321, 2000 | |
| Cells: | Ventricular epithelial |
| Enzyme(s) | Papain: 11.4 u/ml Deoxyribonuclease I: 10 u/ml |
| Medium | DMEM/F12 |
Neural: Human | |
| |
| Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture
, Exp Eye Res 69,
97, 1999 | |
| Cells: | Retinal pigment epithelial (RPE) |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS |
Neural: Human | |
| |
| Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.: Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons
, F.E.B.S. Lett. 462,
117, 1999 | |
| Cells: | Dorsal root ganglion neurons |
| Enzyme(s) | Collagenase Type 1: 0.2% Neutral Protease: 0.5% |
| Medium | DMEM/F-12 |
Neural: Insect | |
| |
| Berger, C., Harzer, H., Burkard, T., Steinmann, J., van der Horst, S., Laurenson, A., Novatchkova, M., Reichert, H. and Knoblich, J.: FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal.
, Cell Rep
2,
407, 2012 | |
| Cells: | Neuroblasts |
| Enzyme(s) | Collagenase Type 1: 0.1% Papain: 0.1% |
| Medium | Rinaldini solution |
Neural: Insect | |
| |
| Sanchez-Soriano, N., Bottenberg, W., Fiala, A., Haessler, U., Kerassoviti, A., Knust, E., Lohr, R. and Prokop, A.: Are Dendrites in Drosophila Homologous to Vertebrate Dendrites?
, Dev Biol 288,
126, 2005 | |
| Cells: | Dendrites |
| Enzyme(s) | Collagenase: 0.05% Neutral Protease: 0.2% |
| Medium | HBSS |
Neural: Insect | |
| |
| Kloppenburg, P. and Horner, M.: Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus
, J Exp Biol
201 (Pt 17),
2529, 1998 | |
| Cells: | Giant interneurons |
| Enzyme(s) | Collagenase: 0.05% Neutral Protease: 0.2% |
| Medium | Leibovitz's L15 |
Neural: Monkey | |
| |
| Kupari, J., Usoskin, D., Parisien, M., Lou, D., Hu, Y., Fatt, M., Lonnerberg, P., Spangberg, M., Eriksson, B., Barkas, N., Kharchenko, P., Lore, K, Khoury, S., Diatchenko, L. and Ernfors, P.: Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain.
, Nat Commun
12,
1510, 2021 | |
| Cells: | Dorsal root ganglia |
| Enzyme(s) | Papain: 35 u/ml Deoxyribonuclease I: 80 u/ml Stemxyme 1: 0.2% |
| Medium | see reference |
Neural: Mouse | |
| |
| Yousuf, M., Samtleben, S., Lamothe, S., Friedman, T., Catuneanu, A., et al.: ER Stress in the Dorsal Root Ganglia Regulates BK Channel Physiology and Contributes to Pain Hypersensitivity in a Mouse Model of Multiple Schlerosis, bioRxivorg
,
, 2020 | |
| Cells: | Dorsal root ganglion |
| Enzyme(s) | Stemxyme 1: 0.1-0.2% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Kramer, N., Haney, M., Morgens, D., Jovicic, A., Couthouis, J., Li, A., Ousey, J., Ma, R., Bieri, G., Tsui, C., Shi, Y., Hertz, N., Tessier-Lavigne, M., Ichida, J., Bassik, M. and Gitler, A.: CRISPR-Cas9 Screens in Human Cells and Primary Neurons Identify Modifiers of C9ORF72 Dipeptide-Repeat-Protein Toxicity.
, Nat Genet
,
, 2018 | |
| Cells: | Cortical neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal |
Neural: Mouse | |
| |
| Mirsky, R. and Jessen, K.: Isolation of Schwann Cell Precursors from Rodents.
, Methods Mol Biol 1739,
3-15, 2018 | |
| Cells: | Schwann cell precursors |
| Enzyme(s) | Collagenase Type 2: 0.2% Hyaluronidase: 0.12% Soybean Trypsin Inhibitor: 0.03% |
| Medium | DMEM/ Ham's F-12 |
Neural: Mouse | |
| |
| Fangmann, L., Teller, S., Stupakov, P., Friess, H., Ceyhan, G. and Demir, I.: 3D Cancer Migration Assay with Schwann Cells.
, Methods Mol Biol 1739,
317-325, 2018 | |
| Cells: | Dorsal root ganglia neurons |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | MEM |
Neural: Mouse | |
| |
| Weng, Y., An, R., Cassin, J., Joseph, J., Mi, R., Wang, C., Zhong, C., Jin, S., Pfeifer, G., Bellacosa, A., Dong, X., Hoke, A., He, Z., Song, H. and Ming, G.: An Intrinsic Epigenetic Barrier for Functional Axon Regeneration
, Neuron 94,
337, 2018 | |
| Cells: | Lumbar DRG |
| Enzyme(s) | Collagenase Type 2: 200 u/ml Neutral Protease: 2.5 u/ml |
| Medium | HBSS |
Neural: Mouse | |
| |
| Meas, S., Nishimura, K., Scheibinger, M. and Dabdoub, A.: Front Neurosci
12,
822, 2018 | |
| Cells: | Spiral ganglion |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | MEM |
Neural: Mouse | |
| |
| Tongtako, W., Lehmbecker, A., Wang, Y., Hahn, K., Baumgartner, W. and Gerhauser, I.: Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties.
, Sci Rep
7,
13915, 2017 | |
| Cells: | Satellite glial |
| Enzyme(s) | Hyaluronidase: 0.2% Collagenase Type 4: 0.2% Collagenase: 0.2% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Xu, W., Sachewsky, N., Azimi, A., Hung, M., Gappasov, A. and Morshead, C.: Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord.
, Stem Cells 35,
485-496, 2017 | |
| Cells: | Neurospheres |
| Enzyme(s) | Trypsin: 0.13% Hyaluronidase: 0.08% |
| Medium | Neurobasal A |
Neural: Mouse | |
| |
| Clements, M., Byrne, E., Camarillo Guerrero, L., Cattin, A., Zakka, L., Ashraf, A., Burden, J., Khadayate, S., Lloyd, A., Marguerat, S. and Parrinello, S.: The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration.
, Neuron 96,
98-114.e7, 2017 | |
| Cells: | Schwann |
| Enzyme(s) | Trypsin: 0.025% Collagenase Type 1: 0.1% Deoxyribonuclease I: 7 u/ml Collagenase Type 2: 0.2% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Sun, H., Tsai, W., Li, B., Tao, W., Chen, P. and Rubart, M.: Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice.
, PLoS ONE
11,
e0148962, 2016 | |
| Cells: | Sympathetic neurons |
| Enzyme(s) | Papain: 10 u/ml Collagenase Type 2: 0.13% Neutral Protease: 0.22% |
| Medium | EBSS |
Neural: Mouse | |
| |
| Choi, H., Sun, D. and Jakobs, T.: Isolation of Intact Astrocytes from the Optic Nerve Head of Adult Mice.
, Exp Eye Res 137,
103-10, 2015 | |
| Cells: | Astrocytes |
| Enzyme(s) | Papain: ~20 u/ml |
| Medium | HBSS |
Neural: Mouse | |
| |
| Beaudet, M., Yang, Q., Cadau, S., Blais, M., Bellenfant, S., Gros-Louis, F. and Berthod, F.: High Yield Extraction of Pure Spinal Motor Neurons, Astrocytes and Microglia from Single Embryo and Adult Mouse Spinal Cord.
, Sci Rep
5,
16763, 2015 | |
| Cells: | Spinal motor neurons, astrocytes, microglia |
| Enzyme(s) | Papain: 0.2% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Ren, H., Lu, T., McGraw, T. and Accili, D.: Anorexia and Impaired Glucose Metabolism in Mice with Hypothalamic Ablation of Glut4 Neurons.
, Diabetes 64,
405-17, 2015 | |
| Cells: | Neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | EBSS |
Neural: Mouse | |
| |
| Lee, B., Cho, H., Jung, J., Yang, Y., Yang, D. and Oh, U.: Anoctamin 1 Contributes to Inflammatory and Nerve-Injury Induced Hypersensitivity.
, Mol Pain
10,
5, 2014 | |
| Cells: | DRG neurons |
| Enzyme(s) | Collagenase Type 2: 0.1% Trypsin: 0.25% |
| Medium | DMEM/F-12 |
Neural: Mouse | |
| |
| Stettner, M., Lohmann, B., Wolffram, K., Weinberger, J., Dehmel, T., Hartung, H., Mausberg, A. and Kieseier, B.: Interleukin-17 Impedes Schwann Cell-Mediated Myelination.
, J Neuroinflammation
11,
63, 2014 | |
| Cells: | Schwann cells |
| Enzyme(s) | Collagenase: 0.05-0.1% Trypsin: 0.125-0.25% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Harris, M., Hulseberg, P., Ling, C., Karman, J., Clarkson, B., Harding, J., Zhang, M., Sandor, A., Christensen, K., Nagy, A., Sandor, Matyas,S. and Fabry, Z.: Immune Privilege of the CNS is not the Consequence of Limited Antigen Sampling.
, Sci Rep
4,
4422, 2014 | |
| Cells: | Oligodendrocytes |
| Enzyme(s) | Papain: 0.1% Deoxyribonuclease I: 0.0002% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Williams, E., Zhong, X., Mohamed, A., Li, R., Liu, Y., Dong, Q., Ananiev, G., Mok, J., Lin, B., Lu, J., Chiao, C., Cherney, R., Li, H., Zhang, S. and Chang, Q: Mutant Astrocytes Differentiated from Rett Syndrome Patients-Specific iPSCs Have Adverse Effects on Wild-Type Neurons.
, Hum Mol Genet
23,
2968-80, 2014 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Neural: Mouse | |
| |
| Stuart, M., Corrigan, F. and Baune, B.: Knockout of CXCR5 Increases the Population of Immature Neural Cells and Decreases Proliferation in the Hippocampal Dentate Gyrus.
, J Neuroinflammation
11,
31, 2014 | |
| Cells: | Neurosperes |
| Enzyme(s) | Papain: 0.1% Deoxyribonuclease I: 0.1% |
| Medium | NeuroCult NSC |
Neural: Mouse | |
| |
| Liu, G., Rustom, N., Litteljohn, D., Bobyn, J., Rudyk, C., Anisman, H. and Hayley, S.: Use of Induced Pluripotent Stem Cell Derived Neurons Engineered to Express BDNF for Modulation of Stressor Related Pathology.
, Front Cell Neurosci
8,
316, 2014 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate |
Neural: Mouse | |
| |
| Masaki, T., Qu, J., Cholewa-Waclaw, J., Burr, K., Raaum, R. and Rambukkana, A.: Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection.
, Cell
152,
51, 2013 | |
| Cells: | Schwann |
| Enzyme(s) | Collagenase Type 1: 0.01% Trypsin: 0.125% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Medina-Rodriguez, E., Arenzana, F., Bribian, A. and de Castro, F.: Protocol to Isolate a Large Amount of Functional Oligodendrocyte Precursor Cells from the Cerebral Cortex of Adult Mice and Humans.
, PLoS ONE
8,
e81620, 2013 | |
| Cells: | Oligodendrocyte precursor cells |
| Enzyme(s) | Papain: 0.09% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Zhang, Y. and Hu, W.: Mouse Enteric Neuronal Cell Culture.
, Methods Mol Biol 1078,
55-63, 2013 | |
| Cells: | Enteric neurons |
| Enzyme(s) | Collagenase Type 4: 0.1% Trypsin: 0.05% Deoxyribonuclease I: 400 u/ml |
| Medium | DMEM/F12 |
Neural: Mouse | |
| |
| Babona-Pilipos, R., Popovic, M. and Morshead, C.: A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field.
, J Vis Exp
68,
e4193, 2012 | |
| Cells: | Neural precursors |
| Enzyme(s) | Trypsin: 0.13% Hyaluronidase: 0.08% |
| Medium | see reference |
Neural: Mouse | |
| |
| Goetz, J. and Trimarchi, J.: Single-Cell Profiling of Developing and Mature Retinal Neurons.
, J Vis Exp
62,
e3824, 2012 | |
| Cells: | Retinal neurons |
| Enzyme(s) | Papain: 10-20 ul |
| Medium | HBSS |
Neural: Mouse | |
| |
| Li, Y., Roy, B., Wang, W., Zhang, L., Sampson, S. and Lin, D.: Imaging pHluorin-Tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons.
, J Vis Exp
69,
e4450, 2012 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 100 ul |
| Medium | DMEM |
Neural: Mouse | |
| |
| Ziegler, A., Schneider, J., Qin, M., Tyler, W., Pintar, J., Fraidenraich, D., Wood, T and Levison, S.: IGF-II Promotes Stemness of Neural Restricted Precursors.
, Stem Cells 30,
1265, 2012 | |
| Cells: | Neuroshere |
| Enzyme(s) | Trypsin: 0.25% Papain: 100 u Deoxyribonuclease I: 0.025% |
| Medium | Pro-N |
Neural: Mouse | |
| |
| Bracko, O., Singer, T., Aigner, S., Knobloch, M., Winner, B., Ray, J., Clemenson, G., Suh, H., Couillard-Despres, S., Aigner, L., Gage, F. and Jessberger, S.: Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis.
, J Neurosci 32,
3376-87, 2012 | |
| Cells: | Neural stem cells |
| Enzyme(s) | Papain: 0.01% Neutral Protease: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F12 |
Neural: Mouse | |
| |
| Salisbury, E., Lazard, Z., Ubogu, E., Davis, A. and Olmsted-Davis, E.: Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2.
, Stem Cells Transl Med 1,
874-85, 2012 | |
| Cells: | Nerve progenitors |
| Enzyme(s) | Collagenase Type 4: 0.025% Trypsin NF 1:250: .025% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Pfaltzgraff, E., Mundell, N. and Labosky, P.: Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube.
, J Vis Exp
,
e4134, 2012 | |
| Cells: | Neural crest |
| Enzyme(s) | Collagenase/Dispase: 0.1% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Pollari, E., Savchenko, E., Jaronen, M., Kanninen, K., Malm, T., Wojciechowski, S., Ahtoniemi, T., Goldsteins, G., Giniatullina, R., Giniatullin, R., Koistinaho, J. and Magga, J.: Granulocyte Colony Stimulating Factor Attenuates Inflammation in a Mouse Model of Amyotrophic Lateral Sclerosis.
, J Neuroinflammation
8,
74, 2011 | |
| Cells: | Spinal cord neurons |
| Enzyme(s) | Papain: 0.05% Deoxyribonuclease I: 0.004% |
| Medium | PBS/DMEM |
Neural: Mouse | |
| |
| O'Meara, R., Ryan, S., Colognato, H. and Kothary, R.: Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues.
, J Vis Exp
54,
3324, 2011 | |
| Cells: | Oligodendrocytes, dorsal root ganglia |
| Enzyme(s) | Papain: 0.15% Deoxyribonuclease I: 0.006% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Conrad, R., Jablonka, S., Sczepan, T., Sendtner, M., Wiese, S. and Klausmeyer, A.: Lectin-Based Isolation and Culture of Mouse Embryonic Motoneurons.
, J Vis Exp
55,
e3200, 2011 | |
| Cells: | Motorneurons |
| Enzyme(s) | Trypsin: 0.025% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Radtke, C., Sasaki, M., Lankford, K., Gallo, V. and Kocsis, J.: CNPase Expression in Olfactory Ensheathing Cells.
, J Biomed Biotechnol
2011,
608496, 2011 | |
| Cells: | Olfactory ensheathing |
| Enzyme(s) | Collagenase: 0.15% Papain: 12 u/ml |
| Medium | DMEM |
Neural: Mouse | |
| |
| Babu, H., Claasen, J., Kannan, S., Runker, A., Palmer, T. and Kempermann, G.: A Protocol for Isolation and Enriched Monolayer Cultivation of Neural Precursor Cells from Mouse Dentate Gyrus.
, Front Neurosci
5,
89, 2011 | |
| Cells: | Dentate gyrus neural precursor |
| Enzyme(s) | Papain: 2.5 u/ml Neutral Protease: 1.0 u/ml Deoxyribonuclease I: 250 u/ml |
| Medium | Neurobasal A |
Neural: Mouse | |
| |
| Milligan, C. and Gifondorwa, D.: Isolation and Culture of Postnatal Spinal Motoneurons.
, Methods Mol Biol 793,
77, 2011 | |
| Cells: | Motoneurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate A |
Neural: Mouse | |
| |
| Brown, J., Gianino, S. and Gutmann, D.: Defective cAMP Generation Underlies the Sensitivity of CNS Neurons to Neurofibromatosis-1 Heterozygosity
, J Neurosci 30,
5579, 2010 | |
| Cells: | Hippocampal and retinal neurons |
| Enzyme(s) | Papain: 1% Deoxyribonuclease I: 5 u/ml |
| Medium | HBSS |
Neural: Mouse | |
| |
| Sayed, B., Christy, A., Walker, M. and Brown, M.: Meningeal Mast Cells Affect Early T Cell Central Nervous System Infiltration and Blood-Brain Barrier Integrity Through TNF: a Role for Neutrophil Recruitment?
, J Immunol 184,
6891-900, 2010 | |
| Cells: | CNS leukocytes |
| Enzyme(s) | Collagenase Type 4: 300 u/ml |
| Medium | HBSS |
Neural: Mouse | |
| |
| Yip, P., Kaan, T., Fenesan, D. and Malcangio, M.: Rapid Isolation and Culture of Primary Microglia from Adult Mouse Spinal Cord.
, J Neurosci Methods 183,
223-37, 2009 | |
| Cells: | Spinal microganglia |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate A |
Neural: Mouse | |
| |
| Radad Khaled, Gille Gabriele, Rausch Wolf-Dieter: Dopaminergic neurons are preferentially sensitive to long-term rotenone toxicity in primary cell culture
, Toxicol In Vitro
22,
68-74, 2008 | |
| Cells: | Dopaminergic neurons |
| Enzyme(s) | Trypsin: 0.1% Deoxyribonuclease I: 0.02% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Pedrola, L., Espert, A., Valdes-Sanchez, T., Sanchez-Piris, M., Sirkowski, E., Scherer, S., Farinas, I., and Palau, F.: Cell Expression of GDAP1 in the Nervous System and Pathogenesis of Charcot-Marie-Tooth Type 4A Disease.
, J Cell Mol Med
12,
679, 2008 | |
| Cells: | DRG neurons |
| Enzyme(s) | Collagenase: 0.2% Trypsin: 0.05% |
| Medium | Ham's F12 |
Neural: Mouse | |
| |
| Brewer Gregory J, Torricelli John R: Isolation and culture of adult neurons and neurospheres
, Nat Protoc
2,
1490-8, 2007 | |
| Cells: | Neurons, neurospheres |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate |
Neural: Mouse | |
| |
| Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24
, Stem Cells 25,
1560-70, 2007 | |
| Cells: | Neural, various |
| Enzyme(s) | Papain: 12 u/ml Trypsin: see reference Collagenase/Dispase: see reference |
| Medium | see reference |
Neural: Mouse | |
| |
| Malin, S., Davis, B. and Molliver, D.: Production of Dissociated Sensory Neuron Cultures and Considerations for their use in Studying Neuronal Function and Plasticity
, Nat Protoc
2,
152, 2007 | |
| Cells: | Sensory neurons, DRG |
| Enzyme(s) | Papain: 20 u/ml Collagenase Type 2: 0.4% Neutral Protease: 0.46% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Eide, L, and McMurray, C: Culture of Adult Mouse Neurons
, Biotechniques
38(1),
99-104, 2005 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Gonzalez John M, Bergmann Cornelia C, Fuss Babette, Hinton David R, Kangas Cindy, Macklin Wendy B, Stohlman Stephen A: Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes
, Glia 51,
22-34, 2005 | |
| Cells: | Brain and spinal cord cells |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS |
Neural: Mouse | |
| |
| Gill JC, Moenter SM, Tsai PS: Developmental regulation of gonadotropin-releasing hormone neurons by fibroblast growth factor signaling
, Endocrinology 145,
3830, 2004 | |
| Cells: | Neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | EBSS |
Neural: Mouse | |
| |
| Okano-Uchida T, Himi T, Komiya Y, and Ishizaki Y: Cerebellar granule cell precursors can differentiate into astroglial cells
, Proc Natl Acad Sci U S A 101,
1211, 2004 | |
| Cells: | Cerebellar granule cell precursors |
| Enzyme(s) | Papain: 16.5 u/ml Deoxyribonuclease I: 0.008% |
| Medium | Dulbecco's PBS |
Neural: Mouse | |
| |
| Savchenko V, Sung U, Blakely RD.: Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody
, Mol Cell Neurosci 24,
1131, 2003 | |
| Cells: | Neurons, ganglia |
| Enzyme(s) | Papain: 20 u/ml Deoxyribonuclease I: 100 u/ml Collagenase: 0.3% Trypsin: 0.05% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Roberts, L., MacDonald, C.and Mark, C.: Anandamide is a Partial Agonist at Native Vanilloid Receptors in Acutely Isolated Mouse Trigeminal Sensory Neurons.
, Br J Pharmacol 137,
421, 2002 | |
| Cells: | Trigeminal senesory neurons |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | HEPES buffered saline |
Neural: Mouse | |
| |
| Deshmukh, M., Kuida, K., and Johnson Jr., E.: Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization
, J Cell Biol
150 (1),
131, 2000 | |
| Cells: | Neurons |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | NGF-containing medium |
Neural: Mouse | |
| |
| Lee, K., Davies, A., and Jaenisch, R.: P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF
, Development
120,
1027, 1994 | |
| Cells: | Neurons, DRG and SCG |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | L-15 medium |
Neural: Mouse | |
| |
| Richards L., Kilpatrick T., and Bartlett P.: De Novo Generation of Neuronal Cells from the Adult Mouse Brain
, Proc Natl Acad Sci U S A 89,
8591, 1992 | |
| Cells: | Neurons, neuronal precursors |
| Enzyme(s) | Trypsin: 0.1% Deoxyribonuclease I: 0.001% |
| Medium | DMEM |
Neural: Mouse | |
| |
| Quinn, S. and De Boni, U.: Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro
, In Vitro Cell Dev Biol 27,
55, 1991 | |
| Cells: | Neurons, dorsal root ganglion |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS |
Neural: Mouse | |
| |
| Kitani, H., Shiurba, R., Sakakura, T., Tomooka, Y.: Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture
, In Vitro Cell Dev Biol 27,
615, 1991 | |
| Cells: | Precursor |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | PBS |
Neural: Mouse | |
| |
| Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.: A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells
, Brain Res 503,
326, 1989 | |
| Cells: | Papillae, taste receptor |
| Enzyme(s) | Pronase E: 0.15% |
| Medium | Carbonate-Phosphate buffer (see reference) |
Neural: Mouse | |
| |
| Varon, S., Skaper, S., Barbin, G., Selak, I., and Manthorpe, M.: Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System
, J Neurosci 4 (3),
654, 1984 | |
| Cells: | PNS test neurons |
| Enzyme(s) | Trypsin: 0.08% |
| Medium | Eagle's Basal Medium (see reference) |
Neural: Mouse | |
| |
| Eagleson, K. and Bennett, M.: Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium
, Neurosci Lett 38,
187, 1983 | |
| Cells: | Neurons, spinal cord |
| Enzyme(s) | Collagenase Type 3: 0.25% |
| Medium | Hank's BSS, CMF |
Neural: Mouse | |
| |
| Shrier, B., Wilson, S., and Nirenberg, M.: Cultured Cell Systems and Methods for Neurobiology
, Vol. 32,, ,
765, 1974 | |
| Cells: | Neural |
| Enzyme(s) | Trypsin NF 1:250: 0.25% |
| Medium | BSS |
Neural: Ovine | |
| |
| Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain
, J Biol Chem 253,
1592, 1978 | |
| Cells: | Oligodendroglia Neural |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | (see reference) |
Neural: Porcine | |
| |
| Si ML, Lee TJ.: Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries
, J Pharmacol Exp Ther 298,
122, 2001 | |
| Cells: | Superior cervical ganglia |
| Enzyme(s) | Papain: 2 u/ml Collagenase: 0.12% Neutral Protease: 0.48% |
| Medium | HBSS |
Neural: Quail | |
| |
| Sieber-Blum, M., and Cohen, A.: Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells
, Dev Biol 80,
96, 1980 | |
| Cells: | Neural crest |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | MEM, HBSS |
Neural: Rat | |
| |
| Mirsky, R. and Jessen, K.: Isolation of Schwann Cell Precursors from Rodents.
, Methods Mol Biol 1739,
3-15, 2018 | |
| Cells: | Schwann cell precursors |
| Enzyme(s) | Collagenase Type 2: 0.2% Hyaluronidase: 0.12% Soybean Trypsin Inhibitor: 0.03% |
| Medium | DMEM/ Ham's F-12 |
Neural: Rat | |
| |
| Andersen, N. and Monje, P.: Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers.
, Methods Mol Biol 1739,
49-66, 2018 | |
| Cells: | Schwann |
| Enzyme(s) | Neutral Protease: 0.25% Collagenase Type 1: 0.05% |
| Medium | DMEM |
Neural: Rat | |
| |
| Fangmann, L., Teller, S., Stupakov, P., Friess, H., Ceyhan, G. and Demir, I.: 3D Cancer Migration Assay with Schwann Cells.
, Methods Mol Biol 1739,
317-325, 2018 | |
| Cells: | Dorsal root ganglia neurons |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | MEM |
Neural: Rat | |
| |
| Maurel, P.: Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies.
, Methods Mol Biol 1739,
17-37, 2018 | |
| Cells: | Schwann |
| Enzyme(s) | Collagenase Type 1: 0.1% Trypsin: 0.25% |
| Medium | DMEM |
Neural: Rat | |
| |
| George, D., Ahrens, P. and Lambert, S.: Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells.
, Glia ,
, 2018 | |
| Cells: | Schwann |
| Enzyme(s) | Stemxyme 1: 0.05% |
| Medium | DMEM |
Neural: Rat | |
| |
| Lin, Y. and Chen, J.: Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release.
, J Vis Exp
,
, 2018 | |
| Cells: | Dorsal root ganglia |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Neural: Rat | |
| |
| Clements, M., Byrne, E., Camarillo Guerrero, L., Cattin, A., Zakka, L., Ashraf, A., Burden, J., Khadayate, S., Lloyd, A., Marguerat, S. and Parrinello, S.: The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration.
, Neuron 96,
98-114.e7, 2017 | |
| Cells: | Schwann |
| Enzyme(s) | Trypsin: 0.025% Collagenase Type 1: 0.1% Deoxyribonuclease I: 7 u/ml Collagenase Type 2: 0.2% |
| Medium | DMEM |
Neural: Rat | |
| |
| Schuh, C., Hercher, D., Stainer, M., Hopf, R., Teuschl, A., Schmidhammer, R. and Redl, H.: Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture.
, Cytotherapy
18,
760-70, 2016 | |
| Cells: | Schwann |
| Enzyme(s) | Collagenase Type 1: 0.05% |
| Medium | DMEM |
Neural: Rat | |
| |
| Hayakawa, K., Esposito, E., Wang, X., Terasaki, Y., Liu, Y., Xing, C., Ji. X. and Lo, E.: Transfer of Mitochondria from Astrocytes to Neurons after Stroke.
, Nature
535,
551-5, 2016 | |
| Cells: | Neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Neural: Rat | |
| |
| Andersen, N., Srinivas, S., Pinero, G. and Monje, P.: A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves.
, Sci Rep
6,
31781, 2016 | |
| Cells: | Schwann |
| Enzyme(s) | Neutral Protease: 0.25% Collagenase Type 1: 0.05% Trypsin: 0.25% |
| Medium | DMEM |
Neural: Rat | |
| |
| Rheaume, C., Cai, B., Wang, J., Fernandez-Salas, E., Aoki, K., Francis, J. and Broide, R.: A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25.
, Toxins 7,
2354-70, 2015 | |
| Cells: | Cortical neurons, DRG |
| Enzyme(s) | PDS kit: per instructions Collagenase Type 1: 0.17% |
| Medium | Neurobasal |
Neural: Rat | |
| |
| Oyanagi, K., Tashiro, T. and Negishi, T.: Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes.
, J Toxicol Sci 40,
459-68, 2015 | |
| Cells: | Cerebral neurons |
| Enzyme(s) | Papain: 2 u/ml Deoxyribonuclease I: 0.01% |
| Medium | DMEM-PBS |
Neural: Rat | |
| |
| Murayama, C., Watanabe, S., Nakamura, M. and, Norimoto, H.: Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons.
, Molecules
20,
14959-69, 2015 | |
| Cells: | Dorsal root ganglia |
| Enzyme(s) | Collagenase Type 3: 0.2% |
| Medium | HBSS |
Neural: Rat | |
| |
| Yu, R., Seymour, V., Berecki, G., Jia, X., Akca, M., Adams, D., Kaas, Q. and Craik, D.: Less is More: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic α-Conotoxin Vc1.1.
, Sci Rep
5,
13264, 2015 | |
| Cells: | Dorsal root ganglion neurons |
| Enzyme(s) | Collagenase Type 2: 0.15% |
| Medium | HBSS |
Neural: Rat | |
| |
| Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord.
, J Vis Exp
,
e52732, 2015 | |
| Cells: | Spinal cord neural progenitor |
| Enzyme(s) | PDS kit: with modifications |
| Medium | EBSS |
Neural: Rat | |
| |
| Wei, X., Yan, J., Tillu, D., Asiedu, M., Weinstein, N., Melemedjian, O., Price, T. and Dussor, G.: Meningeal Norepinephrine Produces Headache Behaviors in Rats via Actions Both on Dural Afferents and Fibroblasts.
, Cephalalgia
35,
1054-64, 2015 | |
| Cells: | Trigeminal ganglion |
| Enzyme(s) | Collagenase Type 2: 0.1% Papain: 20 u/ml |
| Medium | DMEM/F12 |
Neural: Rat | |
| |
| Stettner, M., Lohmann, B., Wolffram, K., Weinberger, J., Dehmel, T., Hartung, H., Mausberg, A. and Kieseier, B.: Interleukin-17 Impedes Schwann Cell-Mediated Myelination.
, J Neuroinflammation
11,
63, 2014 | |
| Cells: | Schwann cells |
| Enzyme(s) | Collagenase: 0.05-0.1% Trypsin: 0.125-0.25% |
| Medium | DMEM |
Neural: Rat | |
| |
| Thurner, P., Gsandtner, I., Kudlacek, O., Choquet, D., Nanoff, C., Freissmuth, M. and Zezula, J.: A Two-State Model for the Diffusion of the A2A Adenosine Receptor in Hippocampal Neurons: Agonist-Induced Switch to Slow Mobility is Modified by Synapse-Associated Protein 102 (SAP102).
, J Biol Chem 289,
9263-74, 2014 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 25 u/ml |
| Medium | L-15 |
Neural: Rat | |
| |
| Loktev, A. and Jackson, P.: Neuropeptide Y Family Receptors Traffic via the Bardet-Biedl Syndrome Pathway to Signal in Neuronal Primary Cilia.
, Cell Rep
5,
1316-29, 2013 | |
| Cells: | Hypothalamic neuronal |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal A |
Neural: Rat | |
| |
| Todd, G., Boosalis, C., Burzycki, A., Steinman, M., Hester, L., Shuster, P. and Patterson, R.: Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons.
, PLoS ONE
8,
e58996, 2013 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate |
Neural: Rat | |
| |
| Yan, J., Melemedjian, O., Price, T. and Dussor, G.: Sensitization of Dural Afferents Underlies Migraine-Related Behavior Following Meningeal Application of Interleukin-6 (IL-6).
, Mol Pain
8,
6, 2012 | |
| Cells: | Trigeminal ganglia |
| Enzyme(s) | Papain: 20 u/ml Collagenase Type 2: 0.3% |
| Medium | HBSS |
Neural: Rat | |
| |
| Ghogha, A., Bruun, D. and Lein, P.: Inducing Dendritic Growth in Cultured Sympathetic Neurons.
, J Vis Exp
61,
e3546, 2012 | |
| Cells: | Sympathetic neurons |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 0.5% |
| Medium | DMEM/F-12 |
Neural: Rat | |
| |
| Bosmans, F., Puopolo, M., Martin-Eauclaire, M., Bean, B., Swartz, K.: Functional Properties and Toxin Pharmacology of a Dorsal Root Ganglion Sodium Channel Viewed through its Voltage Sensors.
, J Gen Physiol 138,
59, 2011 | |
| Cells: | Dorsal root ganglion |
| Enzyme(s) | Papain: 20 u/ml Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | L-15 |
Neural: Rat | |
| |
| Giacomello, M., Girardi, S., Scorzeto, M., Peruffo, A., Maschietto, M., Cozzi, B. and Vassanelli, S.: Stimulation of Ca²+ Signals in Neurons by Electrically Coupled Electrolyte-Oxide-Semiconductor Capacitors.
, J Neurosci Methods 198,
1, 2011 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 0.05% |
| Medium | Neurobasal |
Neural: Rat | |
| |
| East, E., de Oliveira, D., Golding, J. and Phillips, J.: Alignment of Astrocytes Increases Neuronal Growth in Three-Dimensional Collagen Gels and is Maintained Following Plastic Compression to Form a Spinal Cord Repair Conduit.
, Tissue Eng Part A
16,
3173, 2010 | |
| Cells: | Dorsal root ganglia, fibroblast |
| Enzyme(s) | Collagenase: 0.125% |
| Medium | DMEM |
Neural: Rat | |
| |
| Chen, N., Newcomb, J., Garbuzova-Davis, S., Davis Sanberg, C., Sanberg, P. and Willing, A.: Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro.
, Aging Dis 1,
173, 2010 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate A |
Neural: Rat | |
| |
| Peltier, D., Simms, A., Farmer, J and Miller, D.: Human Neuronal Cells Possess Functional Cytoplasmic and TLR-Mediated Innate Immune Pathways Influenced by Phosphatidylinositol-3-Kinase Signaling
, J Immunol 184,
7010, 2010 | |
| Cells: | Neuronal |
| Enzyme(s) | Papain: 0.2% |
| Medium | Neurobasal E |
Neural: Rat | |
| |
| Tanaka, S., Shaikh, I., Chiocca, E. and Saeki, Y.: The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development.
, PLoS ONE
4,
e5922, 2009 | |
| Cells: | Cerebellar granule neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | PBS |
Neural: Rat | |
| |
| Liu, Y., Yohrling, G., Wang, Y., Hutchinson, T., Brenneman, D., Flores, C., Zhao, B.: Carisbamate, a Novel Neuromodulator, Inhibits Voltage-Gated Sodium Channels and Action Potential Firing of Rat Hippocampal Neurons.
, Epilepsy Res
83,
66, 2009 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | Neurobasal/B27 |
Neural: Rat | |
| |
| Yokosuka Makoto, Ohtani-Kaneko Ritsuko, Yamashita Kayoko, Muraoka Daisuke, Kuroda Yoichiro, Watanabe Chiho: Estrogen and environmental estrogenic chemicals exert developmental effects on rat hypothalamic neurons and glias
, Toxicol In Vitro
22,
1-9, 2008 | |
| Cells: | Hypothalamic neurons and glias |
| Enzyme(s) | Papain: 0.5 u/ml |
| Medium | DMEM |
Neural: Rat | |
| |
| Sakisaka, T., Yamamoto, Y., Mochida, S., Nakamura, M., Nishikawa, K., Ishizaki, H., Okamoto-Tanaka, M., Miyoshi, J., Fujiyoshi, Y., Manabe, T. and Takai, Y.: Dual Inhibition of SNARE Complex Formation by Tomosyn Ensures Controlled Neurotransmitter Release.
, J Cell Biol
183,
323, 2008 | |
| Cells: | Superior cervical ganglion |
| Enzyme(s) | Collagenase: 0.05% |
| Medium | L-15 |
Neural: Rat | |
| |
| Frank, L., Caldera-Siu, A. and Pothos, E.: Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates.
, J Vis Exp
21,
820, 2008 | |
| Cells: | Dopamine neurons |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | PBS |
Neural: Rat | |
| |
| Obradovic Darja, Gronemeyer Hinrich, Lutz Beat, Rein Theo: Cross-talk of vitamin D and glucocorticoids in hippocampal cells
, J Neurochem 96,
500-9, 2006 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal A |
Neural: Rat | |
| |
| Lacroix-Fralish, M., Tawfik, V., Nutile-McMenemy, N., Harris, B. and Deleo, J.: Differential Regulation of Neuregulin 1 Expression by Progesterone in Astrocytes and Neurons.
, Neuron Glia Biol
2,
227, 2006 | |
| Cells: | astrocytes |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Neural: Rat | |
| |
| Mothe, A., Kulbatski, I., Van Bendegem, R., Lee, L., Kobayashi, E., Keating, A., and Tator, C.: Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells
, J Histochem Cytochem 53(10),
1215, 2005 | |
| Cells: | Spinal cord progenitor cells
Hybrid toxins |
| Enzyme(s) | PDS kit: see reference |
| Medium | Neurobasal A |
Neural: Rat | |
| |
| Gavva NR, Tamir R, Qu Y, Klionsky L, Zhang TJ, Immke D, Wang J, Zhu D, Vanderah TW, Porreca F, Doherty EM, Norman MH, Wild KD, Bannon AW, Louis JC, Treanor JJ.: AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties
, J Pharmacol Exp Ther 313,
474, 2005 | |
| Cells: | Dorsal root ganglia |
| Enzyme(s) | PDS kit: per instructions |
| Medium | MEM/Ham's F12 |
Neural: Rat | |
| |
| Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M: Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons
, Chem Senses 30,
111, 2005 | |
| Cells: | Vomeronasal receptor neurons |
| Enzyme(s) | Collagenase/Dispase: 0.1% Papain: 0.5 u/ml |
| Medium | DMEM/F12 |
Neural: Rat | |
| |
| Floyd Candace L, Gorin Fredric A, Lyeth Bruce G: Mechanical strain injury increases intracellular sodium and reverses Na+/Ca2+ exchange in cortical astrocytes
, Glia 51,
35-46, 2005 | |
| Cells: | Cortical astrocytes |
| Enzyme(s) | Papain: see reference |
| Medium | DMEM |
Neural: Rat | |
| |
| Pedraza Carlos E, Podlesniy Petar, Vidal Noemi, Arevalo Juan Carlos, Lee Ramee, Hempstead Barbara, Ferrer Isidre, Iglesias Montse, Espinet Carme: Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR
, Am J Pathol 166,
533-43, 2005 | |
| Cells: | Superior cervical ganglion |
| Enzyme(s) | Collagenase Type 4: 20 u/ml Trypsin: 0.25% |
| Medium | DMEM |
Neural: Rat | |
| |
| Connor, M, Naves, L. and McCleskey, E.: Contrasting Phenotypes of Putative Proprioceptive and Nociceptive Trigeminal Neurons Innervating Jaw Muscle in Rat.
, Mol Pain
1,
31, 2005 | |
| Cells: | Trigeminal neurons |
| Enzyme(s) | Papain: 20 u/ml Collagenase: 0.3% |
| Medium | CMF Hanks |
Neural: Rat | |
| |
| Lovshin JA, Huang Q, Seaberg R, Brubaker PL, Drucker DJ: Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells
, Endocrinology 145,
3495, 2004 | |
| Cells: | Brainstem and cortical neurons |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM |
Neural: Rat | |
| |
| Hu Hong-Zhen, Gu Qihai, Wang Chunbo, Colton Craig K, Tang Jisen, Kinoshita-Kawada Mariko, Lee Lu-Yuan, Wood Jackie D, Zhu Michael X: 2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3
, J Biol Chem 279,
35741-8, 2004 | |
| Cells: | Dorsal root ganglion neurons |
| Enzyme(s) | Collagenase Type 4: 0.125% Trypsin: 0.05% |
| Medium | DMEM/Ham's F12 |
Neural: Rat | |
| |
| Lin CR, Wu PC, Shih HC, Cheng JT, Lu CY, Chou AK, Yang LC.: Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain
, Cell Transplant 11,
17, 2002 | |
| Cells: | Spinal progenitor cells |
| Enzyme(s) | PDS kit: with modifications |
| Medium | Neurobasal medium |
Neural: Rat | |
| |
| Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.: The adult substantia nigra contains progenitor cells with neurogenic potential
, J Neurosci 22,
6639, 2002 | |
| Cells: | Adult progenitor |
| Enzyme(s) | Papain: 2.5 u/ml Deoxyribonuclease I: 250 u/ml Neutral Protease: 1 u/ml |
| Medium | DMEM/F-12 |
Neural: Rat | |
| |
| Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, and Shaw G: Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem
, J Neurophysiol 87,
1076, 2002 | |
| Cells: | Neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate A |
Neural: Rat | |
| |
| Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity.
, Neuron 35,
643, 2002 | |
| Cells: | Sciatic nerve and gut neural crest stem |
| Enzyme(s) | Collagenase Type 4: 0.025% Trypsin: 0.005% Deoxyribonuclease I: 0.05% |
| Medium | HBSS |
Neural: Rat | |
| |
| Liu, Q., Kawai, H., and Berg, D.: B-Amyloid Peptide Blocks the Response of a7-Containing Nicotinic Receptors on Hippocampal Neurons
, PNAS
98 (8),
4734, 2001 | |
| Cells: | Neurons, hippocampal |
| Enzyme(s) | Papain: 15 - 20 u/ml |
| Medium | Eagle's MEM (see reference) |
Neural: Rat | |
| |
| Acosta, C., Fabrega, A., Masco, D., and Lopez, H.: A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development
, J Neurosci 21 (22),
8873, 2001 | |
| Cells: | Neurons |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | MEM10 |
Neural: Rat | |
| |
| Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M, Matsumoto M: Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo
, J Neurosci 21,
9204-13, 2001 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | PDS kit: see reference |
| Medium | DMEM |
Neural: Rat | |
| |
| O'Connor, S., Andreadis, J., Shaffer, K., Ma, W., Pancrazio, J., and Stenger, D.: Immobilization of Neural Cells in Three-Dimensional Matrices for Biosensor Applications
, Biosensors & Bioelectronics 14,
871, 2000 | |
| Cells: | Cortical |
| Enzyme(s) | Papain: |
| Medium | Neurobasal medium and DMEM |
Neural: Rat | |
| |
| Neuhoff, H., Roeper, J., Schweizer, M.: Activity-Dependent Formation of Perforated Synapses in Cultured Hippocampal Neurons
, Eur J Neurosci 11,
4241, 1999 | |
| Cells: | Neurons, hippocampal |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | EBSS |
Neural: Rat | |
| |
| Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells
, Cell
96,
737, 1999 | |
| Cells: | Sciatic nerves |
| Enzyme(s) | Trypsin: 0.025% Collagenase Type 3: 0.1% |
| Medium | L-15 medium (see reference) |
Neural: Rat | |
| |
| Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells
, Cell
96,
737, 1999 | |
| Cells: | Sciatic nerves |
| Enzyme(s) | Trypsin: 0.025% Collagenase Type 3: 0.1% |
| Medium | L-15 medium (see reference) |
Neural: Rat | |
| |
| Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., and Frisen, J: Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System
, Cell
96,
25, 1999 | |
| Cells: | Spinal cord |
| Enzyme(s) | Trypsin: 0.133% |
| Medium | HBSS and PIPES |
Neural: Rat | |
| |
| Wilding, T., and Huettner, J.: Activation and Desensitization of Hippocampal Kainate Receptors
, J Neurosci 17 (8),
2713, 1997 | |
| Cells: | Neurons, hippocampal |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | EBSS |
Neural: Rat | |
| |
| Schafer, K., Saffrey, M., Burnstock, G., and Mastres-Ventura, P.: A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract
, Brain Res Proto 1,
109, 1997 | |
| Cells: | Myenteric ganglia |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | MEM-HEPES |
Neural: Rat | |
| |
| Hall, R., and Soderling, T.: Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons
, J Biol Chem 272 (7),
4135, 1997 | |
| Cells: | Neurons, hippocampal |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | Harvest buffer |
Neural: Rat | |
| |
| Davies, S., Fitch, M., Memberg, S., Hall, A, Raisman, G., and Silver, J.: Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System
, Nature
390,
680, 1997 | |
| Cells: | Neurons, DRG |
| Enzyme(s) | Neutral Protease: 0.5% |
| Medium | L-15 w/ CO2 |
Neural: Rat | |
| |
| Twitchell, W., Brown, S., and Mackie, K.: Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons
, J Neurophysiol 78,
43, 1997 | |
| Cells: | Neurons, hippocampal |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | MEM |
Neural: Rat | |
| |
| Brewer, G.J.: Isolation and culture of adult rat hippocampal neurons of any age
, J Neurosci Methods 71,
143, 1997 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | HibernateA/B27 |
Neural: Rat | |
| |
| Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL: Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons
, J Neurosci 16,
2912-23, 1996 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 20 u/ml Deoxyribonuclease I: 0.01% |
| Medium | MEM |
Neural: Rat | |
| |
| Robertson, S., Rae, M., Rowan, E. and Kennedy, C.: Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia.
, Br J Pharmacol 118,
951, 1996 | |
| Cells: | DRG neurons |
| Enzyme(s) | Papain: 20 u/ml Collagenase Type 1: 150 u/ml Neutral Protease: 0.8% |
| Medium | HBSS |
Neural: Rat | |
| |
| McFarlane, S., and Cooper, E.: Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons
, J Neurosci 13 (6),
2591, 1993 | |
| Cells: | Neurons, sympathetic |
| Enzyme(s) | Neutral Protease: 0.24% |
| Medium | HBSS |
Neural: Rat | |
| |
| Allen, T., Sim, J., and Brown, D.: The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat
, J Physiol
460,
91, 1993 | |
| Cells: | Basal forebrain neurons |
| Enzyme(s) | Trypsin: 0.125% |
| Medium | Gey's BSS |
Neural: Rat | |
| |
| Rayport, S., Sulzer, D., Shi, W., Sawasdikosol, S., Monaco, J., Batson, D., and Rajendran, G.: Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology
, J Neurosci 12 (11),
4264, 1992 | |
| Cells: | Postnatal dopamine neurons |
| Enzyme(s) | Trypsin: 0.035% |
| Medium | (see reference) |
Neural: Rat | |
| |
| Cheng, B., and Mattson, M.: IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage
, J Neurosci 12 (4),
1558, 1992 | |
| Cells: | Hippocampal |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | HBSS |
Neural: Rat | |
| |
| Stemple, D., and Anderson, D.: Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest
, Cell
71,
973, 1992 | |
| Cells: | Stem, neural crest |
| Enzyme(s) | Collagenase: 0.075% |
| Medium | Ringer's solution |
Neural: Rat | |
| |
| Buchhalter, J., and Dichter, M.: Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus
, Brain Res Bull 26,
333, 1991 | |
| Cells: | Pyramidal neurons Nonpyramidal neurons |
| Enzyme(s) | Trypsin: 0.027% |
| Medium | HEPES |
Neural: Rat | |
| |
| Mithen, F., Reiker, M., and Birchem, R.: Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture
, In Vitro Cell Dev Biol 26,
129, 1990 | |
| Cells: | Schwann, dorsal root ganglia |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS, CMF |
Neural: Rat | |
| |
| Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons
, In Vitro Cell Dev Biol 26,
335, 1990 | |
| Cells: | Heart Adrenal chromaffin Paraneurons |
| Enzyme(s) | Trypsin: 0.06% |
| Medium | 25mM HEPES buffered Locke's solution, CMF |
Neural: Rat | |
| |
| Novelli, A., Reilly, J., Lysko, P., and Henneberry, R.: Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced
, Brain Res 451,
205, 1988 | |
| Cells: | Cerebellar neurons |
| Enzyme(s) | Trypsin: 0.025% |
| Medium | Eagle's MEM |
Neural: Rat | |
| |
| Mattson, M., and Kater, S.: Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda
, Int J Dev Neurosci 6 (5),
439, 1988 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | Eagle's MEM |
Neural: Rat | |
| |
| Hatanaka,H., Tsukui, H., Nihonmatsu, I.: Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor
, Neurosci Lett 79,
85, 1987 | |
| Cells: | Septal neurons |
| Enzyme(s) | Papain: 0.05% |
| Medium | PBS, CMF |
Neural: Rat | |
| |
| Wood, P., and Bunge, R.: Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals
, Nature
320,
756, 1986 | |
| Cells: | CNS cells |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | EBSS |
Neural: Rat | |
| |
| Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats
, J Neurosci 6,
3044, 1986 | |
| Cells: | Neurons, visual cortex |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | BSS (see reference) |
Neural: Rat | |
| |
| Jahr, C. and Jessell, T.: Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate
, J Neurosci 5,
2281, 1985 | |
| Cells: | Dorsal horn neurons Spinal |
| Enzyme(s) | Trypsin: 0.025% |
| Medium | Ham's F-12 |
Neural: Rat | |
| |
| Bartlett, W. and Banker, G.: An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts
, J Neurosci 4,
1944, 1984 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | HBSS, CMF |
Neural: Rat | |
| |
| Leifer, D., Lipton, S., Barnstable, C., and Masland, R.: Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture
, Science 224,
303, 1984 | |
| Cells: | Ganglion, retina |
| Enzyme(s) | Papain: 12.5 u/ml |
| Medium | HBSS w/5 mM HEPES |
Neural: Rat | |
| |
| Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat
, J Neurosci 3 (12),
2532, 1983 | |
| Cells: | Retina |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Ham's F-12 |
Neural: Rat | |
| |
| Raff, M., Fields, K., Hakomori, S., Mirsky, R., Pruss, R., and Winter, J.: Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture
, Brain Res 174,
283, 1979 | |
| Cells: | Neurons and glial |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | MEM (see reference) |
Neural: Rat | |
| |
| Wakshull, E., Johnson, M., Burton, H.: Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons
, J Neurophysiol 42,
1410, 1979 | |
| Cells: | Neurons, sympathetic |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | L-15 or HBSS, CMF |
Neural: Rat | |
| |
| Brockes, J., Fields, K., and Raff, M.: Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve
, Brain Res 165,
105, 1979 | |
| Cells: | Schwann |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Neural: Rat | |
| |
| Dichter, M.: Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation
, Brain Res 149,
279, 1978 | |
| Cells: | Neurons, cortical |
| Enzyme(s) | Trypsin: 0.027% |
| Medium | MEM |
Neural: Rat | |
| |
| Reichardt, L., Patterson, P.: Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures
, Nature
270,
147, 1977 | |
| Cells: | Neurons, sympathetic |
| Enzyme(s) | Collagenase Type 1: 0.01% |
| Medium | Hank's solution, CF |
Neural: Rat | |
| |
| Mains, R., and Patterson, P.: Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties
, J Cell Biol
59,
329, 1973 | |
| Cells: | Neurons, superior cervical ganglia |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Basal L-15 medium |
Neural: Salamander | |
| |
| Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase
, J Cell Biol
100,
175, 1985 | |
| Cells: | Retina |
| Enzyme(s) | Papain: 14 u/ml |
| Medium | Saline |
Neural: Salamander | |
| |
| Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina
, Proc Natl Acad Sci U S A 75,
3507, 1978 | |
| Cells: | Photoreceptors, retina |
| Enzyme(s) | Papain: 0.05% |
| Medium | (see reference) |
Neural: Shellfish | |
| |
| Lee, A., Decourt, B. and Suter, D.: Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones.
, J Vis Exp
12,
662, 2008 | |
| Cells: | Neurons |
| Enzyme(s) | Neutral Protease: 1.0% |
| Medium | L-15-ASW |
Neural: Shellfish | |
| |
| Zoran, M., Doyle, R. and Haydon, P.: Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis
, Neuron 6,
145, 1991 | |
| Cells: | Buccal ganglia; SLT muscle |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | DMEM |
Neural: Shellfish | |
| |
| Haydon, P.: The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata
, J Neurosci 8,
1032, 1988 | |
| Cells: | Somata, buccal ganglia |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | Antibiotic saline, Leibowitz 50% |
Neural: Shellfish | |
| |
| Cohan, C., Haydon, P., and Kater, S.: Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma
, J Neurosci Res 13,
285, 1985 | |
| Cells: | Buccal ganglia |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | L-15 medium |
Neural: Shellfish | |
| |
| Schacher, S., and Proshanksy, E.: Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment
, J Neurosci 3 (12),
2403, 1983 | |
| Cells: | Neurons LUQ cells |
| Enzyme(s) | Protease: 1% |
| Medium | L15 medium |
Neural: Shellfish | |
| |
| Camardo, J., Proshansky, E., and Schacher, S.: Identified Aplysia Neurons Form Specific Chemical Synapses in Culture
, J Neurosci 3 (12),
2614, 1983 | |
| Cells: | LUQ cells RUQ cells |
| Enzyme(s) | Protease: 1% |
| Medium | L15 medium |
Neural: Turtle | |
| |
| Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors
, Proc Natl Acad Sci U S A 69,
1987, 1972 | |
| Cells: | Retinal |
| Enzyme(s) | Papain: 0.1% (13.5 u/mg) |
| Medium | Kreb's Ringer |
Pancreas: Bovine | |
| |
| Cotton, C., and Al-Nakkash, L.: Isolation and Culture of Bovine Pancreatic Duct Epithelial cells
, Am J Physiol 272,
G1328, 1997 | |
| Cells: | Duct epithelial |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HEPES |
Pancreas: Bovine | |
| |
| Sato, T., Sato, M., Hudson, E., and Jones, R.: Characterization of Bovine Pancreatic Ductal Cells Isolated by a Perfusion-Digestion Technique
, In Vitro 19,
651, 1983 | |
| Cells: | Ductal |
| Enzyme(s) | Neutral Protease: 0.05% |
| Medium | EBSS |
Pancreas: Bovine | |
| |
| Stiles, G., and Lefkowitz, R.: Hormone-Sensitive Adneylate Cyclase
, J Biol Chem 257 (11),
6287, 1982 | |
| Cells: | Platelets |
| Enzyme(s) | Trypsin: |
| Medium | (see reference) |
Pancreas: Canine | |
| |
| Zoran, M., Doyle, R. and Haydon, P.: Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis
, Neuron 6,
145, 1991 | |
| Cells: | Buccal ganglia; SLT muscle |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | DMEM |
Pancreas: Canine | |
| |
| Noel, J., Rabinovitch, A., Olson, L., Kyriakides, G., Miller, J., and Mintz, D.: A Method for Large-Scale, High-Yield Isolation of Canine Pancreatic Islets of Langerhans
, Metabolism 31 (2),
184, 1982 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 600-1100 u/ml Deoxyribonuclease I: 10 ug/ml |
| Medium | RPMI 1640 |
Pancreas: Fish | |
| |
| Schrezenmeir, J., Laue, C., Sternheim,E., Wolbert, K., Darquy, S., Chicheportiche, D., Kirchgessner, J., and Reach, G.: Long-Term Function of Single-Cell Preparations of Piscine Principal Islets in Hollow Fibers
, Transplant Proc 24 (6),
2941, 1992 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.12 - 0.46 u/ml |
| Medium | RPMI 1640 |
Pancreas: Guinea-Pig | |
| |
| Schultz, G., Sarras, Jr, M., Gunther, G., Hull, B., Alicea, H., Gorelick, F., and Jamieson, J.: Guinea Pig Pancreatic Acini Prepared with Purified collagenase
, Exp Cell Res
130,
49, 1980 | |
| Cells: | Acinar |
| Enzyme(s) | Collagenase Type 3: 60 u/ml |
| Medium | Kreb's Ringer |
Pancreas: Guinea-Pig | |
| |
| Gardner, J., Conlon, T., Klaeveman, H., Adams, T., and Ondetti, M.: Action of Cholecystokinin and Cholinergic Agents on Calcium Transport in Isolated Pancreatic Acinar Cells
, J Clin Invest 56,
366, 1975 | |
| Cells: | Acinar |
| Enzyme(s) | Soybean Trypsin Inhibitor: 0.01% |
| Medium | Kreb's Ringer |
Pancreas: Guinea-Pig | |
| |
| Amsterdam, J., and Jamieson, J.: Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells
, Proc Natl Acad Sci U S A 69 (10),
3028, 1972 | |
| Cells: | Exocrine |
| Enzyme(s) | Hyaluronidase: 0.15% - 0.2% |
| Medium | Kreb's Ringer |
Pancreas: Hamster | |
| |
| Feldman, J., and Chapman, B.: Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique
, Acta Diabetol 12,
208, 1975 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 1.3% - 2.0% |
| Medium | HBSS |
Pancreas: Human | |
| |
| Brissova, M., Haliyur, R., Saunders, D., Shrestha, S., Dai, C., Blodgett, D., Bottino, R., Campbell-Thompson, M., Aramandla, R., Poffenberger, G., Lindner, J., Pan, F., von Herrath, M., Greiner, D. and Shultz, L.: α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes.
, Cell Rep
22,
2667-2676, 2018 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 1: 1,600u/isolation Neutral Protease: 200 u/isolation Deoxyribonuclease I: 12,000 u/isolation |
| Medium | RPMI |
Pancreas: Human | |
| |
| Cane, M., Sutton, R. and Criddle, D.: Isolation of Human Pancreatic Acinar Cells From Rat and Human Pancreas, Pancreapedia
20,
1, 2011 | |
| Cells: | Acinar |
| Enzyme(s) | CLSPA: 200 u/ml Soybean Trypsin Inhibitor: 0.01% |
| Medium | See Reference |
Pancreas: Human | |
| |
| Li, C., Heidt, D., Dalerba, P., Burant, C., Zhang, L., Adsay, V., Wicha, M., Clarke, M. and Simeone, D.: Identification of Pancreatic Cancer Stem Cells.
, Cancer Res 67,
1030, 2007 | |
| Cells: | Pancreatic cancer stem cells |
| Enzyme(s) | Collagenase Type 4: 200 u/ml |
| Medium | medium 199 |
Pancreas: Human | |
| |
| Contractor, H., Johnson, P., Chadwick, D., Robertson, G., and London, N.: The Effect of UW Solution and its Components on the Collagenase Digestion of Human and Porcine Pancreas
, Cell Transplant 4,
615, 1995 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.4% |
| Medium | HBSS |
Pancreas: Human | |
| |
| Watt, P., Mullen, Y., Benhamou, P., Hober, C., Nomura, Y., Watanabe, Y., Passaro, E., Zinner, M., and Brunicardi, F.: Simplified Semiautomated Method For Isolating Islets From the Human Pancreas
, Transplant Proc 26 (2),
582, 1994 | |
| Cells: | Islets |
| Enzyme(s) | Trypsin: Trypsin: 0.125-0.25% Collagenase: 0.2% Deoxyribonuclease I: 200 u/ml |
| Medium | Eurocollins solution |
Pancreas: Human | |
| |
| Warnock, G., Rajotte, R., Evans, M., Ellis, D., DeGroot, T., and Dawidson, I.: Isolation of Islets of Langerhans Following Cold Storage of Human Pancreas
, Transplant Proc XIX (4),
3466, 1987 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.6% |
| Medium | Eurocollins solution |
Pancreas: Human | |
| |
| Izumi, R., Konishi, K., Ueno, K., Shimizu, K., Hirosawa, H., Takahashi, N., and Miyazaki, I.: Isolation of Human Pancreatic Islets from Cryopreserved Pancreas, Transplant Proc XVII,
383, 1985 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.8% |
| Medium | HBSS |
Pancreas: Human | |
| |
| Gray, D., McShane, P., Grant, A., and Morris, P.: A Method for Isolation of Islets of Langerhans from the Human Pancreas
, Diabetes 33,
1055, 1984 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase (1 or 4): 0.60% |
| Medium | HBSS |
Pancreas: Human | |
| |
| Sutherland, D., Matas, A., Steffes, M., and Najarian, J.: Infant Human Pancreas: A Potential Source of Islet Tissue for Transplantation
, Diabetes 25 (12),
1123, 1976 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 170-210 u/ml |
| Medium | HBSS |
Pancreas: Monkey | |
| |
| Scharp, D., Murphy, J., Newton, W., Ballinger, W., and Lacy, P.: Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats, Transplant Proc 7,
739, 1975 | |
| Cells: | Islets |
| Enzyme(s) | Hyaluronidase: 0.05% |
| Medium | HBSS |
Pancreas: Mouse | |
| |
| Low, L., Zavortink, M., Mitchell, J., Gan, W., Do, O., Schwiening, C., Gaisano, H. and Thorn, P.: Insulin Secretion from Beta Cells in Intact Mouse Islets is Targeted Towards the Vasculature
, Diabetologia 57,
1655, 2014 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | HBSS |
Pancreas: Mouse | |
| |
| Koh, D., Moody, M. and Jo, J.: Collection of Islets of Langerhans using an Equilibrium Method.
, Biotechniques
55,
34-7, 2013 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.1-0.25% |
| Medium | HBSS |
Pancreas: Mouse | |
| |
| Huch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis.
, EMBO J
32,
2708-21, 2013 | |
| Cells: | Pancreas organoid |
| Enzyme(s) | Collagenase: 0.012% Neutral Protease: 0.012% |
| Medium | DMEM |
Pancreas: Mouse | |
| |
| Greggio, C., De Franceschi, F., Figueiredo-Larsen, M., Gobaa, S., Ranga, A., Semb, H., Lutolf, M. and Grapin-Botton, A.: Artificial Three-Dimensional Niches Deconstruct Pancreas Development In Vitro.
, Development
140,
4452-62, 2013 | |
| Cells: | Pancreatic progenitor |
| Enzyme(s) | Neutral Protease: 0.125% |
| Medium | DMEM |
Pancreas: Mouse | |
| |
| Bertera, S., Balamurugan, A., Bottino, R., He, J. and Trucco, M.: Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin.
, J Transplant
2012,
856386, 2012 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | RPMI 1640 |
Pancreas: Mouse | |
| |
| Ding, G., Wang, F., Shu, J., Tian, S., Jiang, Y., Zhang, D., Wang, N., Luo, Q., Zhang, Y., Jin, F., Leung, P., Sheng, J. and Huang, H.: Transgenerational Glucose Intolerance with Igf2/H19 Epigenetic Alterations in Mouse Islet Induced by Intrauterine Hyperglycemia.
, Diabetes 61,
1133-42, 2012 | |
| Cells: | Islet |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | RPMI-1640 |
Pancreas: Mouse | |
| |
| Kobayashi, T., Yamaguchi, T., Hamanaka, S., Kato-Itoh, M., Yamazaki, Y., Ibata, M., Sato, H., Lee, Y., Usui, J., Knisely, A., Hirabayashi, M. and Nakauchi, H.: Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells.
, Cell
142,
787, 2010 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | RPMI 1640 |
Pancreas: Mouse | |
| |
| Taguchi, Y., Tasaki, Y., Terakado, K., Kobayashi, K., Machida, T. and Kobayashi, T.: Impaired Insulin Secretion from the Pancreatic Islets of Hypothyroidal Growth-Retarded Mice.
, J Endocrinol 206,
195-204, 2010 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | HBSS |
Pancreas: Mouse | |
| |
| Ji, B., Gaiser, S., Chen, X., Ernst, S. and Logsdon, C.: Intracellular Trypsin Induces Pancreatic Acinar Cell Death but not NF-KappaB Activation.
, J Biol Chem 284,
17488, 2009 | |
| Cells: | Ancinar |
| Enzyme(s) | CLSPA: see reference Soybean Trypsin Inhibitor: 0.001% |
| Medium | DMEM |
Pancreas: Mouse | |
| |
| Huang, H., Xie, Q., Kang, M., Zhang, B., Zhang, H., Chen, J., Zhai, C., Yang, D., Jiang, B. and Wu, Y.: Labeling Transplanted Mice Islet with Polyvinylpyrrolidone Coated Superparamagnetic Iron Oxide Nanoparticles for In Vivo Detection By Magnetic Resonance Imaging.
, Nanotechnology
20,
365101, 2009 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | RPMI 1540 |
Pancreas: Mouse | |
| |
| Li, D., Yuan, Y., Tu, H., Liang, Q. and Dai, L.: A Protocol for Islet Isolation from Mouse Pancreas.
, Nat Protoc
4,
1649, 2009 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 1,000 u/ml |
| Medium | RPMI 1640 |
Pancreas: Mouse | |
| |
| Carter, J., Dula, S., Corbin, K., Wu, R. and Nunemaker, C.: A Practical Guide to Rodent Islet Isolation and Assessment.
, Biol Proced Online 11,
3, 2009 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.14% |
| Medium | RPMI 1640 |
Pancreas: Mouse | |
| |
| Szot, G., Koudria, P. and Bluestone, J.: Murine Pancreatic Islet Isolation.
, J Vis Exp
7,
255, 2007 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.03-0.08% |
| Medium | RPMI 1640 |
Pancreas: Mouse | |
| |
| Wang, Y., Soyombo, A., Shcheynikov, N., Zeng, W., Dorwart, M., Marino, C., Thomas, P. and Muallem, S.: Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis.
, EMBO J
25,
5049, 2006 | |
| Cells: | Pancreatic ductal |
| Enzyme(s) | CLSPA: 50 u/ml Hyaluronidase: 400 u/ml Soybean Trypsin Inhibitor: 0.02% |
| Medium | DMEM |
Pancreas: Mouse | |
| |
| Voronina, S., Barrow, S., Gerasimenko, O., Petersen, O. and Tepikin, A.: Effects of Secretagogues and Bile Acids on Mitochondrial Membrane Potential of Pancreatic Acinar Cells: Comparison of Different Modes of Evaluating Delta Psi.
, J Biol Chem 279,
27327, 2004 | |
| Cells: | Acinar |
| Enzyme(s) | CLSPA: 200 u/ml |
| Medium | see reference |
Pancreas: Mouse | |
| |
| Astrof, S., Crowley, D., George, E., Fukuda, T., Sekiguchi, K., Hanahan, D. and Hynes, R.: Direct Test of Potential Roles of EIIIA and EIIIB Alternatively Spliced Segments of Fibronectin in Physiological and Tumor Angiogenesis.
, Mol Cell Biol
24,
8662-70, 2004 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | RPMI |
Pancreas: Mouse | |
| |
| Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard: The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells
, J Cell Sci
116,
1463-9, 2003 | |
| Cells: | Pancreatic islet |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | HBSS |
Pancreas: Mouse | |
| |
| Wu Yulian, Han Bing, Luo Hongyu, Roduit Raphael, Salcedo TheodoraW, Moore PaulA, Zhang Jun, Wu Jiangping: DcR3/TR6 effectively prevents islet primary nonfunction after transplantation
, Diabetes 52,
2279-86, 2003 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | HBSS |
Pancreas: Mouse | |
| |
| Kurup, S., and Bhonde, R.: Analysis and Optimization of Nutritional Set-up for Murine Pancreatic Acinar Cells
, JOP
3 (1),
8, 2002 | |
| Cells: | Acinar |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Waymouth's MB |
Pancreas: Mouse | |
| |
| Koster, J., Marshall, B., Ensor, N., Corbett, J., and Nichols, C.: Targeted Overativity of Cell KATP Channels Induces Profound Neonatal Diabetes
, Cell
100,
645, 2000 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | CF Medium |
Pancreas: Mouse | |
| |
| Strowski M, Parmar R, Blake A, Schaeffer J: Somatostatin inhibits insulin and glucagon secretion via two receptors subtypes: an in vitro study of pancreatic islets from somatostatin receptor 2 knockout mice
, Endocrinology 141,
111-7, 2000 | |
| Cells: | Pancreatic islets |
| Enzyme(s) | Collagenase Type 4: 0.4% |
| Medium | Gey's BSS |
Pancreas: Mouse | |
| |
| Toivola, D., Ku, N., Ghori, N., Lowe, A., Michie, S. and Omary, M.: Effects of Keratin Filament Disruption on Exocrine Pancreas-Stimulated Secretion and Susceptibility to Injury.
, Exp Cell Res
255,
156, 2000 | |
| Cells: | Acinar cells and acini |
| Enzyme(s) | Collagenase Type 1: see reference CLSPA: see reference |
| Medium | see reference |
Pancreas: Mouse | |
| |
| Fogarty, K., Kidd, J., Tuft, R. and Thorn, P.: A Bimodal Pattern of InsP(3)-Evoked Elementary Ca(2+) Signals in Pancreatic Acinar Cells.
, Biophys J
78,
2298, 2000 | |
| Cells: | Acinar |
| Enzyme(s) | CLSPA: see reference |
| Medium | see reference |
Pancreas: Mouse | |
| |
| Githens, S: Pancreatic Duct Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12B:12.1, 1995 | |
| Cells: | Duct |
| Enzyme(s) | Papain: 25 u/ml |
| Medium | DMEM /F-12 |
Pancreas: Mouse | |
| |
| Jauch, P., Peterson, O., and Lauger, P.: Electrogenic Properties of the Na-Alanine Cotransporter in Acinar Cells
, J Membr Biol 94,
99, 1986 | |
| Cells: | Acinar |
| Enzyme(s) | Collagenase: 100 u/ml |
| Medium | HEPES |
Pancreas: Mouse | |
| |
| Dalpe-Scott, M., Heick, H., and Begin-Heick, N.: Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release
, Diabetes 32,
932, 1983 | |
| Cells: | Islets |
| Enzyme(s) | Hyaluronidase: 0.5% |
| Medium | Kreb's Ringer bicarbonate buffer |
Pancreas: Mouse | |
| |
| Burnham DB, Williams JA: Effects of carbachol, cholecystokinin, and insulin on protein phosphorylation in isolated pancreatic acini
, J Biol Chem 257,
10523-8, 1982 | |
| Cells: | Acinar |
| Enzyme(s) | CLSPA: 70-90 u/ml Soybean Trypsin Inhibitor: 0.01% |
| Medium | Krebs-Henseleit |
Pancreas: Mouse | |
| |
| Yesil, P, Michel, M., Chwalek, K., Pedack, S., Jany, C., Ludwig, B., Bornstein, S. and Lammert, E.: A New Collagenase Blend Increases the Number of Islets Isolated from Mouse Pancreas.
, Islets
1,
185, | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: see reference |
| Medium | DMEM |
Pancreas: Porcine | |
| |
| Van der Burg Michael P M, Graham John M: Iodixanol Density Gradient Preparation in University of Wisconsin solution for porcine islet purification
, ScientificWorldJournal
3,
1154-9, 2003 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: see reference |
| Medium | Univ of Wisconsin solution |
Pancreas: Porcine | |
| |
| Zhao, X., Han, J., and Tang, C.: Primary Culture of Porcine Pancreatic Acinar Cells
, JOP
2 (2),
78, 2001 | |
| Cells: | Acinar |
| Enzyme(s) | Collagenase Type 3: 200 u/ml |
| Medium | RPMI 1640 |
Pancreas: Porcine | |
| |
| Korbutt, G., Elliott, J., Ao, Z., Smith, D., Warnock, G., and Rajotte, R.: Large Scale Isolation, Growth, and Function of Porcine Neonatal Islet Cells
, J Clin Invest 97 (9),
2119, 1996 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.25% |
| Medium | HBSS |
Pancreas: Porcine | |
| |
| Johnson, P., van Suylichem, P., Roberts, D., Vos-Scheperkeuter, G., White, S., van Schlifgaarde, R., London, N.: Design of a Simple, in vitro Method for Evaluation of the Efficiency of Crude Clostridium histolyticum Collagenase and its Components for Porcine Islet Isolation, Xenotransplantation 2,
165, 1995 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.1%-0.2% |
| Medium | HBSS |
Pancreas: Porcine | |
| |
| Brandhorst, D., Brandhorst, H., Hering, B., Federlin, K., and Bretzel, R.: Islet Isolation from the Pancreas of Large Mammals and Humans: 10 Years of Experience
, Exp Clin Endocrinol 103,
3, 1995 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HBSS |
Pancreas: Porcine | |
| |
| Heiser, A., Ulrichs, K., and Muller-Ruchholtz, W.: Isolation of Porcine Pancreatic Islets: Low Trypsin Activity During the Isolation Procedure Guarantees Reproducible High Islet Yields
, J Clin Lab Anal 8,
407, 1994 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | HBSS |
Pancreas: Porcine | |
| |
| Ricordi, C., Socci, C., Davalli, A., Staudacher, C., Baro, P., Vertova, A., Sassi, I., Gavazzi, F., Pozza, G., and Di Carlo, V.: Isolation of the Elusive Pig Islet
, Surgery 107 (6),
688, 1990 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | HBSS |
Pancreas: Porcine | |
| |
| Iwatsuki, N., and Peterson, O.: Action of Tetraethylammonium on Calcium-Activated Potassim Channels in Pig Pancreatic Acinar Cells Studied by Patch-Clamp Single-Channel and Whole-Cell Current Recording
, J Membr Biol 86,
139, 1985 | |
| Cells: | Acinar |
| Enzyme(s) | Collagenase: 100 u/ml |
| Medium | Saline |
Pancreas: Rabbit | |
| |
| Renckens, B., Schrijen, J., Swarts, H., DePont, J., and Bonting, S.: Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas
, Biochim Biophys Acta 544,
338, 1978 | |
| Cells: | Acinar |
| Enzyme(s) | Hyaluronidase: 0.2% |
| Medium | Kreb's Ringer bicarbonate buffer |
Pancreas: Rabbit | |
| |
| Feldman, J., and Chapman, B.: Preparation of Islets of Langerhans from Rabbits and Hamsters by the Collagenase Digestion Technique
, Acta Diabetol 12,
208, 1975 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 1.3% - 2.0% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Li, J., Zhou, R., Bie, B., Huang, N., Guo, Y., Chen, H., Shi, M., Yang, J., Zhang, J. and Li, Z.: Emodin and Baicalein Inhibit Sodium Taurocholate-Induced Vacuole Formation in Pancreatic Acinar Cells.
, World J Gastroenterol
24,
35-45, 2018 | |
| Cells: | Acinar |
| Enzyme(s) | Collagenase: 0.01% Soybean Trypsin Inhibitor: 0.001% |
| Medium | Culture media |
Pancreas: Rat | |
| |
| Verga Falzacappa, C., Mangialardo, C., Raffa, S., Mancuso, A., Piergrossi, P., Moriggi, G., Piro, S., Stigliano, A., Torrisi, M., Brunetti, E., Toscano, V. and Misiti, S.: The Thyroid Hormone T3 Improves Function and Survival of Rat Pancreatic Islets During In Vitro Culture.
, Islets
2,
96, 2010 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | CMRL 1066 |
Pancreas: Rat | |
| |
| Williams, J.: Isolation of Rodent Pancreatic Acinar Cells and Acini by Collagenase Digestion, Pancreapedia
,
, 2010 | |
| Cells: | Acinar |
| Enzyme(s) | CLSPA: 100 u/ml |
| Medium | DMEM |
Pancreas: Rat | |
| |
| Williams, J., Huang, H., Kover, K., Moore, W., Berkland, C., Singh, M., Smirnova, I., MacGregor, R. and Stehno-Bittel, L.: Reduction of Diffusion Barriers in Isolated Rat Islets Improves Survival, but not Insulin Secretion or Transplantation Outcome.
, Organogenesis 6,
115, 2010 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 1: 450 u/ml |
| Medium | Leibowitz L-15 |
Pancreas: Rat | |
| |
| Getty-Kaushik, L., Richard, A., Deeney, J., Shirihai, O. and Corkey, B.: The CB1 Antagonist Rimonabant Decreases Insulin Hypersecretion in Rat Pancreatic Islets
, Obesity
17,
1856, 2009 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: see reference |
| Medium | RPMI 1640 |
Pancreas: Rat | |
| |
| MacGregor, R., Williams, S., Tong, P., Kover, K., Moore, W. and Stehno-Bittel, L.: Small Rat Islets are Superior to Large Islets in In Vitro Function and in Transplantation Outcomes.
, Am J Physiol/Endo 290,
E771, 2006 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 1: 450 u/ml |
| Medium | RPMI 1640 |
Pancreas: Rat | |
| |
| Tian XH, Xue WJ, Ding XM, Pang XL, Teng Y, Tian PX, and Feng XS: Small intestinal submucosa improves islet survival and function during in vitro culture
, World J Gastroenterol
11,
7378, 2005 | |
| Cells: | Pancreatic islets |
| Enzyme(s) | Collagenase: 0.75% |
| Medium | RPMI 1640 |
Pancreas: Rat | |
| |
| Haefliger Jacques-Antoine, Tawadros Thomas, Meylan Laure, Gurun SabineLe, Roehrich Marc-Estienne, Martin David, Thorens Bernard, Waeber Gerard: The scaffold protein IB1/JIP-1 is a critical mediator of cytokine-induced apoptosis in pancreatic beta cells
, J Cell Sci
116,
1463-9, 2003 | |
| Cells: | Pancreatic islet |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Blinman TA, Gukovsky I, Mouria M, Zaninovic V, Livingston E, Pandol SJ, Gukovskaya AS: Activation of pancreatic acinar cells on isolation from tissue: cytokine upregulation via p38 MAP kinase
, Am J Physiol Cell Physiol
279,
C1993-2003, 2000 | |
| Cells: | Pancreatic acini |
| Enzyme(s) | CLSPA: 30 u/ml Collagenase Type 4: 30 u/ml Soybean Trypsin Inhibitor: 0.01% |
| Medium | M199 |
Pancreas: Rat | |
| |
| Ji B, Kopin AS, Logsdon CD: Species differences between rat and mouse CCKA receptors determine the divergent acinar cell response to the cholecystokinin analog JMV-180
, J Biol Chem 275,
19115-20, 2000 | |
| Cells: | Ancinar |
| Enzyme(s) | CLSPA: see reference Soybean Trypsin Inhibitor: 0.01% |
| Medium | DMEM |
Pancreas: Rat | |
| |
| Verspohl, E., and Wienecke, A.: The Role of Protein Kinase C in the Desensitization of Rat Pancreatic Islets to Chrolinergic stimulation
, J Endocrinol 159,
287, 1998 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 126 - 196 u/ml |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Takaki, R and Ono J: Culture of Pancreatic Islet Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12B:11.1, 1995 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.5 - 0.9% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Githens, S: Pancreatic Duct Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12B:12.1, 1995 | |
| Cells: | Duct |
| Enzyme(s) | Papain: 25 u/ml |
| Medium | DMEM /F-12 |
Pancreas: Rat | |
| |
| Hirschi, K., Kenny, S., Justice, J., Brannon, P.: Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells
, In Vitro Cell Dev Biol 27,
660, 1991 | |
| Cells: | Acinar |
| Enzyme(s) | Hyaluronidase: 462 u/ml |
| Medium | Ham's F12 |
Pancreas: Rat | |
| |
| Yeh, C., Mertz, P., Oliver, C., Baum, B., and Kousvelari, E.: Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells
, In Vitro Cell Dev Biol 27,
707, 1991 | |
| Cells: | Parotid acinar |
| Enzyme(s) | Trypsin: 0.001% |
| Medium | F12 medium |
Pancreas: Rat | |
| |
| Foskett, J., Roifman, C. and Wong, D.: Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells
, J Biol Chem 266,
2778, 1991 | |
| Cells: | Parotid acinar |
| Enzyme(s) | Trypsin: 0.02% |
| Medium | Solution B (See reference) |
Pancreas: Rat | |
| |
| Ohzato, H., Gotoh, M., Monden, M., Dono, K., Kanai, T., and Mori, T.: Improvement in the Islet Yield From a Cold-Preserved Pancreas by Pancreatic Ductal Collagenase Distention at the Time of Harvesting
, Transplantation 51,
566, 1991 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.1% - 0.2% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Githens, S., Schexnayder, J., Desai, K., and Patke, C.: Rat Pancreatic Interlobular Duct Epithelium: Isolation and Culture in Collagen Gel
, In Vitro Cell Dev Biol 25 (8),
679, 1989 | |
| Cells: | Interlobular ducts |
| Enzyme(s) | Papain: 25 u/ml |
| Medium | DMEM/Ham's F-12 |
Pancreas: Rat | |
| |
| Menozzi, D., Sato, S., Jensen, R., and Gardner, J.: Cyclic GMP Does Not Inhibit Protein Kinase C-Mediated Enzyme Secretion in Rat Pancreatic Acini
, J Biol Chem 264,
995, 1989 | |
| Cells: | Acinar |
| Enzyme(s) | Soybean Trypsin Inhibitor: 0.01% |
| Medium | HEPES |
Pancreas: Rat | |
| |
| Melvin, J., Kawaguchi, M., Baum, B., and Turner, R.: A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells
, Biochem Biophys Res Commun
145,
754, 1987 | |
| Cells: | Acinar, parotid |
| Enzyme(s) | Hyaluronidase: 0.015% |
| Medium | Earle's MEM |
Pancreas: Rat | |
| |
| Oliver, C., Waters, J., Tolbert, C., and Kleinman, H.: Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel
, In Vitro Cell Dev Biol 23,
465, 1987 | |
| Cells: | Acinar Exorbital lacrimal, parotid, pancreas |
| Enzyme(s) | Trypsin: 0.01% |
| Medium | HBSS, CMF |
Pancreas: Rat | |
| |
| Tsao, M., and Duguid, W.: Establishment of Propagable Epithelial Cell Lines From Normal Adult Rat Pancreas
, Exp Cell Res
168,
365, 1987 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Ham's F-12/HBSS (see reference) |
Pancreas: Rat | |
| |
| Gotoh, M., Maki, T., Satomi, S., Porter, J., Bonner-Weir, S., O'Hara, C., and Monaco, A.: Reproducible High Yield of Rat Islets By Stationary In Vitro Digestion Following Pancreatic Ductal or Portal Venous Collagenase Injection
, Transplantation 43 (5),
725, 1987 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Quissell, D., Redman, R., and Mark, M.: Short-Term Primary Culture of Acinar-Intercalated Duct Complexes From Rat Submandibular Glands
, In Vitro Cell Dev Biol 22,
469, 1986 | |
| Cells: | Acinar, submandibular gland |
| Enzyme(s) | Hyaluronidase: 0.1 % |
| Medium | HBSS, CF |
Pancreas: Rat | |
| |
| Sutton, R., Peters, M., McShane, P., Gray, D., and Morris, P.: An Improved Method for the Isolation of Islets of Langerhans From the Adult Rat Pancreas, Transplant Proc XVII (6),
1819, 1986 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | Hank's solution |
Pancreas: Rat | |
| |
| Brannon, P., Orrison, B., and Kretchmer, N.: Primary Cultures of Rat Pancreatic Acinar Cells in Serum-Free Medium
, In Vitro Cell Dev Biol 21 (1),
6, 1985 | |
| Cells: | Acinar |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | HBSS (see reference) |
Pancreas: Rat | |
| |
| Githens, S., Holmquist, D., Whelan, J., and Ruby, J.: Ducts of the Rat Pancreas in Agarose Matrix Culture
, In Vitro 16,
797, 1980 | |
| Cells: | Duct |
| Enzyme(s) | Trypsin: 0.01% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Schulz, I., Heil, K., Kribben, A., Sachs, G., and Haase, W.: Isolation and Functional Characterization of Cells From the Exocrine Pancreas,Biology of Normal and Cancerous Exocrine Pancreatic Cells,Ribet, A., Pradayrol, L., and Susini, C., Elsevier,
, 1980 | |
| Cells: | Exocrine |
| Enzyme(s) | Hyaluronidase: 0.9% |
| Medium | Kreb's Ringer |
Pancreas: Rat | |
| |
| Katada, T., and Ui, M.: Enhanced Insulin Secretion and Cyclic Amp Accumulation in Pancreatic Islets Due to Activation of Native Calcium Ionophores
, J Biol Chem 254 (2),
469, 1979 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 1% |
| Medium | Medium 199 |
Pancreas: Rat | |
| |
| Williams, J., Korc, M., and Dormer, R.: Action of Secretagogues on a New Preparation of Functionally Intact, Isolated Pancreatic Acini
, Am J Physiol 235,
517, 1978 | |
| Cells: | Acinar |
| Enzyme(s) | Hyaluronidase: 0.18% |
| Medium | Kreb's Henseleit bicarbonate buffer |
Pancreas: Rat | |
| |
| Wolters, G., Konijenendijk, W., and Bouman, P.: Effects of Fasting on Insulin Secretion, Islet Glucose Metabolism, and the Cyclic Adenosine 3'5'-Monophosphate Content of Rat Pancreatic Islets In Vitro
, Diabetes 26 (6),
530, 1977 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Kreb's Ringer bicarbonate buffer |
Pancreas: Rat | |
| |
| Shibata, A., Ludvigsen, C., Naber, S., McKaneil M., and Lacy, P.: Standardization of a Digestion-Filtration Method for Isolation of Islets
, Diabetes 25,
667, 1976 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.5% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Kondo, S., and Schulz, I.: Calcium Ion Uptake in Isolated Pancreas Cells Induced by Secretagogues
, Biochim Biophys Acta 419,
76, 1976 | |
| Cells: | Exocrine |
| Enzyme(s) | Hyaluronidase: 0.15% |
| Medium | Krebs |
Pancreas: Rat | |
| |
| Braaten, J., Jarlfors, U., Smith, D., and Mintz, D.: Purification of Monolayer Cell Cultures of the Endocrine Pancreas
, Tissue Cell
7 (4),
747, 1975 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 0.63% |
| Medium | EBSS (see reference) |
Pancreas: Rat | |
| |
| Mangos, J., McSherry, N., Butcher, F., Irwin, K., and Barber, T.: Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character)
, Am J Physiol 229 (3),
560, 1975 | |
| Cells: | Acinar, parotid |
| Enzyme(s) | Trypsin: 0.01% Collagenase: 40-50 u/ml Hyaluronidase: 0.10% |
| Medium | HBSS CMF |
Pancreas: Rat | |
| |
| Leonard, R., Lazarow, A., and Hegre, O.: Pancreatic Islet Transplantation in the Rat
, Diabetes 22,
413, 1973 | |
| Cells: | Islets |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Puck's saline buffered w/ EDTA 0.02% |
Pancreas: Rat | |
| |
| Lacy, P., Walker, M., and Fink, J.: Perifusion of Isolated Rat Islets in Vitro
, Diabetes 21 (10),
987, 1972 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Hanks solution |
Pancreas: Rat | |
| |
| Ballinger, W., and Lacy, P.: Transplantation of Intact Pancreatic Islets in Rats
, Surgery 72 (2),
175, 1972 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Lacy, P., and Kostianovsky, M.: Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas
, Diabetes 16,
35, 1967 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase Type 4: 1.0% - 1.2% |
| Medium | HBSS |
Pancreas: Rat | |
| |
| Essner, E.: Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands
, Exp Cell Res
7,
430, 1954 | |
| Cells: | Ascites hepatoma |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Phosphate buffer (see reference) |
Parotid: Mouse | |
| |
| Ogawa, M., Oshima, M., Imamura, A., Sekine, Y., Ishida, K., Yamashita, K., Nakajima, K., Hirayama, M., Tachikawa, T. and Tsuji, T.: Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ.
, Nat Commun
4,
2498, 2013 | |
| Cells: | Parotid and sublingual glandular |
| Enzyme(s) | Neutral Protease: 50 u/ml Collagenase Type 1: 100 u/ml |
| Medium | PBS |
Parotid: Rat | |
| |
| Looms, D., Dissing, S., Tritsaris, K., Pedersen, A. and Nauntofte, B.: Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells.
, Adv Dent Res
14,
62, 2000 | |
| Cells: | Acinar |
| Enzyme(s) | Collagenase: 75 u/ml Hyaluronidase: 153 u/ml |
| Medium | RPMI 1640 |
Parotid: Rat | |
| |
| D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL: Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes
, J Histochem Cytochem 45,
965-73, 1997 | |
| Cells: | Parotid acinar cells |
| Enzyme(s) | Collagenase Type 2: 90 u/ml |
| Medium | Krebs-Henseleit Bicarbonate |
Parotid: Rat | |
| |
| Prasad, K.N., Edwards-Prasad, J., Carvaiho, I., LaRosa, F.G., Baibinder, E., Meyers, A., and Quissell, D.: Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments
, In Vitro Cell Dev Biol 28,
493, 1992 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 50-75 u/ml Hyaluronidase: 0.1% |
| Medium | HBSS CF |
Parotid: Rat | |
| |
| Yeh, C., Mertz, P., Oliver, C., Baum, B., and Kousvelari, E.: Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells
, In Vitro Cell Dev Biol 27,
707, 1991 | |
| Cells: | Parotid acinar |
| Enzyme(s) | Trypsin: 0.001% |
| Medium | F12 medium |
Parotid: Rat | |
| |
| Foskett, J., Roifman, C. and Wong, D.: Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells
, J Biol Chem 266,
2778, 1991 | |
| Cells: | Parotid acinar |
| Enzyme(s) | Trypsin: 0.02% |
| Medium | Solution B (See reference) |
Parotid: Rat | |
| |
| Takuma, T. and Ichida, T.: Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP
, J Invest Dermatol 103,
95, 1988 | |
| Cells: | Parotid |
| Enzyme(s) | Hyaluronidase: 0.025% |
| Medium | HBSS with 20mM HEPES |
Parotid: Rat | |
| |
| Melvin, J., Kawaguchi, M., Baum, B., and Turner, R.: A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells
, Biochem Biophys Res Commun
145,
754, 1987 | |
| Cells: | Acinar, parotid |
| Enzyme(s) | Hyaluronidase: 0.015% |
| Medium | Earle's MEM |
Parotid: Rat | |
| |
| Oliver, C., Waters, J., Tolbert, C., and Kleinman, H.: Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel
, In Vitro Cell Dev Biol 23,
465, 1987 | |
| Cells: | Acinar Exorbital lacrimal, parotid, pancreas |
| Enzyme(s) | Trypsin: 0.01% |
| Medium | HBSS, CMF |
Parotid: Rat | |
| |
| Mangos, J., McSherry, N., Butcher, F., Irwin, K., and Barber, T.: Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character)
, Am J Physiol 229 (3),
560, 1975 | |
| Cells: | Acinar, parotid |
| Enzyme(s) | Trypsin: 0.01% Collagenase: 40-50 u/ml Hyaluronidase: 0.10% |
| Medium | HBSS CMF |
Pituitary: Bovine | |
| |
| Hassan, H., and Merkel, R.: Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells
, In Vitro Cell Dev Biol 30A,
435, 1994 | |
| Cells: | Pituitary |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | DMEM |
Pituitary: Bovine | |
| |
| Mason, W. and Ingram, C.: Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells
, Vol. 124,, ,
207, 1986 | |
| Cells: | Pituitary |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | EBSS, CMF |
Pituitary: Bovine | |
| |
| Ferrara, N., Goldsmith, P., Fujii, D., and Weiner, R.: Culture and Characterization of Follicular Cells of the Bovine Anterior Pituitary and Pars Tuberalis
, Vol. 124,, ,
245, 1986 | |
| Cells: | Follicular, anterior pituitary and pars tuberalis |
| Enzyme(s) | Deoxyribonuclease I: 200 µg/ml |
| Medium | HBSS, CMF |
Pituitary: Bovine | |
| |
| Ridgway, E., Klibanski, A., Marorana, M., Milbury, P., Kieffer, J., and Chin, W.: The Effect of Somatostatin on the Release of Thyrotropin and its Subunits from Bovine Anterior Pitituary Cells in Vitro
, Endocrinology 112 (6),
1937, 1983 | |
| Cells: | Pitituary |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | DMEM |
Pituitary: Mouse | |
| |
| Pyczek, J., Buslei, R., Schult, D., Holsken, A., Buchfelder, M., Hess, I., Hahn, H. and Uhmann, A.: Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland.
, Sci Rep
6,
24928, 2016 | |
| Cells: | Pituitary |
| Enzyme(s) | Collagenase Type 1: 1,000 u/ml Soybean Trypsin Inhibitor: 0.01% Hyaluronidase: 0.1% Deoxyribonuclease I: 0.001% |
| Medium | DMEM |
Pituitary: Mouse | |
| |
| Perez Millain, M., Brinkmeier, M., Mortensen, A. and Camper, S.: PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells.
, Elife
5,
, 2016 | |
| Cells: | Pituitary |
| Enzyme(s) | Collagenase Type 2: 0.5% Deoxyribonuclease I: 0.005% |
| Medium | HBSS |
Pituitary: Mouse | |
| |
| Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A: Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase
, Endocrinology 144,
188-200, 2003 | |
| Cells: | Pituitary |
| Enzyme(s) | Collagenase: 0.4% Hyaluronidase: 0.1% Trypsin: 0.3% |
| Medium | DMEM/Han's F12 |
Pituitary: Ovine | |
| |
| Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen: Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes
, Endocrinology 143,
4609-19, 2002 | |
| Cells: | Somatotropes |
| Enzyme(s) | Collagenase Type 1: 0.3% Hyaluronidase: |
| Medium | Medium 199 |
Pituitary: Rat | |
| |
| Akieda-Asai, S., Zaima, N., Ikegami, K., Kahyo, T, Yao, I., Hatanaka, T., Iemura, S., Sugiyama, R., Yokozeki, T., Eishi, Y., Koike, M., Ikeda, K., Chiba, T., Yamaza, H., Shimokawa, I., Song, S., Matsuno, A., Mizutani, A., Sawabe, M. Chao, N., Tanaka, M., : SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals.
, PLoS ONE
5,
e11755, 2010 | |
| Cells: | Pituitary |
| Enzyme(s) | Collagenase Type 2: 0.4% Deoxyribonuclease I: 0.04% |
| Medium | DMEM |
Pituitary: Rat | |
| |
| Zhou, X., De Schepper, J., De Craemer, D., Delhase, M., Gys, G., Smitz, J., and Hooghe-Peters, E.: Pituitary Growth Hormone Release and Gene Expression in Cafeteria-Diet-Induced Obese Rats
, J Endocrinol 159,
165, 1998 | |
| Cells: | Pituitary |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | DMEM |
Pituitary: Rat | |
| |
| D'Emden, M. and Wark, J.: Culture Requirements for Optimal Expression of 1,25-Dihydroxyvitamin D3-Enhanced Thyrotropin Secretion, In Vitro Cell Dev Biol 27,
197, 1991 | |
| Cells: | Anterior pituitary gland |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | EBSS, CMF |
Pituitary: Rat | |
| |
| Wilfinger, W., Larsen, W., Downs, T., and Wilbur, D.: An In Vitro Model for Studies of Intercellular Communication in Cultured Rat Anterior Pituitary Cells
, Tissue Cell
16 (4),
483, 1984 | |
| Cells: | Pituitary |
| Enzyme(s) | Trypsin: 0.3 % |
| Medium | HEPES |
Pituitary: Rat | |
| |
| Portanova, R., Smith, D., and Sayers, G.: A Trypsin for the Preparation of Isolated Rat Anterior Pituitary
, Proc Soc Exp Biol Med 133,
573, 1970 | |
| Cells: | Anterior pituitary |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Krebs |
Prostate: Human | |
| |
| Taylor, R., Toivanen, R., Frydenberg, M., Pedersen, J., Harewood, L., Collins, A., Maitland, N. and Risbridger, G.: Human Epithelial Basal Cells are Cells of Origin of Prostate Cancer, Independent of CD133 Status.
, Stem Cells 30,
1087, 2012 | |
| Cells: | Prostatic cancer fibroblasts |
| Enzyme(s) | Collagenase: 225 u/ml Hyaluronidase: 125 u/ml |
| Medium | RPMI 1640 |
Prostate: Human | |
| |
| Le Hanh, Arnold Julia T, McFann Kimberly K, Blackman Marc R: DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells
, Am J Physiol/Endo 290,
E952-60, 2006 | |
| Cells: | Prostatic stromal cells |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F-12 |
Prostate: Human | |
| |
| Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi: Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R
, Am J Pathol 165,
533-40, 2004 | |
| Cells: | Prostate stromal cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | RPMI 1640 |
Prostate: Human | |
| |
| Levine AC, Liu XH, Greenberg PD, Eliashvili M, Schiff JD, Aaronson SA, Holland JF, Kirschenbaum A: Androgens induce the expression of vascular endothelial growth factor in human fetal prostatic fibroblasts
, Endocrinology 139,
4672-8, 1998 | |
| Cells: | Prostatic fibroblasts |
| Enzyme(s) | Collagenase Type 1: 0.125% |
| Medium | DMEM/F12 |
Prostate: Mouse | |
| |
| Burger, P., Gupta, R., Xiong, X., Ontiveros, C., Salm, S., Moscatelli, D. and Wilson, E.: High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells.
, Stem Cells 27,
2220-8, 2009 | |
| Cells: | Prostate epithelial/stem |
| Enzyme(s) | Collagenase Type 2: 0.5% Trypsin: 0.05% |
| Medium | HBSS |
Prostate: Mouse | |
| |
| Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake: Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate
, Proc Natl Acad Sci U S A 101,
9375-80, 2004 | |
| Cells: | Prostatic epithelial |
| Enzyme(s) | Collagenase Type 3: 170 u/ml |
| Medium | DMEM |
Prostate: Mouse | |
| |
| Dubey P, Wu H, Reiter RE, Witte ON: Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression
, Cancer Res 61,
3256-61, 2001 | |
| Cells: | Prostatic stem |
| Enzyme(s) | Collagenase Type 1: 170 u/ml |
| Medium | DMEM |
Reproductive: Bovine | |
| |
| Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R: Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis
, Endocrinology 142,
5254-60, 2001 | |
| Cells: | Corpus leuteal cells |
| Enzyme(s) | Collagenase Type 4: 420 u/ml |
| Medium | M-199 |
Reproductive: Bovine | |
| |
| Tsang PC, Poff JP, Boulton EP, Condon WA: Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro
, Biol Reprod 53,
1160-8, 1995 | |
| Cells: | Leuteal |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | Ham's F-12 |
Reproductive: Bovine | |
| |
| Coplen, D., Howard, P., Duckett, J., Snyder, H., and Macarak, E.: Characterization of a Fibroblast Cell From the Urinary Bladder Wall
, In Vitro Cell Dev Biol 30A,
604, 1994 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Medium 199 |
Reproductive: Bovine | |
| |
| Munson, L., Chandler, S., and Schlafer, D.: Long-Term Culture of Bovine Trophoblastic Cells, J Tiss Cul Meth 11 (3),
123, 1988 | |
| Cells: | Epithelial Endometrial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM/EBSS |
Reproductive: Bovine | |
| |
| Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues
, Endocr Res 10,
151, 1984 | |
| Cells: | Interna & corpus luteum Endometrium Ovarian Uterine |
| Enzyme(s) | Pronase: 0.1% |
| Medium | Moscona's BSS |
Reproductive: Canine | |
| |
| Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells.
, PLoS ONE
7,
e44693, 2012 | |
| Cells: | Amniotic membrane MSC |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 0.2% |
| Medium | LG-DMEM |
Reproductive: Chicken | |
| |
| Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong: An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles
, J Reprod Dev
52,
569-76, 2006 | |
| Cells: | Primary follicles |
| Enzyme(s) | Trypsin: 0.15% Collagenase Type 1: 0.125% |
| Medium | Dulbecco's phosphate buffered saline |
Reproductive: Fish | |
| |
| Ryu, J., Kim, H., Bae, S., Jung, C. and Gong, S.: Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of
the Rockfish, Sebastes schlegeli, Fish Aquatic Sci 19,
, 2016 | |
| Cells: | Ovarian |
| Enzyme(s) | Collagenase Type 1: 500 u/ml |
| Medium | L-15 |
Reproductive: Frog | |
| |
| Nicoll, G, Jawad, A., Weymouth, R, Zhang, H. and Beg, A.: Pharmacological characterization of the excitatory ‘Cys-loop’ GABA receptor family in Caenorhabditis elegans
, Br J Pharmacol 174,
781, 2017 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | Barth's solution |
Reproductive: Frog | |
| |
| Chatzidaki, A., D'Oyley, J., Gill-Thind, J., Sheppard, T. and Millar, N.: The Influence of Allosteric Modulators and Transmembrane Mutations on Desensitisation and Activation of alpha7 Nicotinic Acetylcholine Receptors.
, Neuropharmacol 97,
75-85, 2015 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Barth's solution |
Reproductive: Frog | |
| |
| O'Connell, D., Mruk, K., Rocheleau, J. and Kobertz, W.: Xenopus laevis Oocytes Infected with Multi-drug-resistant Bacteria: Implications for Electrical Recordings.
, J Gen Physiol 138,
271-7, 2011 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | see reference |
Reproductive: Frog | |
| |
| Mruk, K. and Kobertz, W.: Discovery of a Novel Activator of KCNQ1-KCNE1 K Channel Complexes.
, PLoS ONE
4,
e4236, 2009 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | see reference |
Reproductive: Frog | |
| |
| Cohen, S., Au, S. and Pante, N.: Microinjection of Xenopus Laevis Oocytes.
, J Vis Exp
24,
1106, 2009 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase: 0.5% |
| Medium | Barth's solution, CF |
Reproductive: Frog | |
| |
| Pannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio: Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species
, J Biol Chem 277,
8912-9, 2002 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | see reference |
Reproductive: Frog | |
| |
| Alagem, N., Dvir, M., and Reuveny, E.: Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues
, J Physiol
534 (2),
381, 2001 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | CF Medium |
Reproductive: Frog | |
| |
| Tian, J., Kim, S., Heilig, E., and Ruderman, J.: Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation
, Proc Natl Acad Sci U S A 97,
14358, 2000 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase Type 1: 1% |
| Medium | (see reference) |
Reproductive: Frog | |
| |
| Karkanias, N, and Papke, R.: Subtype-Specific Effects of Lithium on Glutamate Receptor Function
, J Neurophysiol 81,
1506, 1999 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Barth's solution, CF |
Reproductive: Frog | |
| |
| Moriarty, T., Gillo, B., Carty, D., Premont, R., Landau, E., Iyengar, R.: Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C
, Proc Natl Acad Sci U S A 85,
8865, 1988 | |
| Cells: | Oocytes |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | CF Medium |
Reproductive: Hamster | |
| |
| Wallis, R., and Drickamer, K.: Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins
, J Biol Chem 274 (6),
3580, 1999 | |
| Cells: | Ovary |
| Enzyme(s) | Trypsin: 5% |
| Medium | Dialyzed fetal calf serum, 10% and 0.5M Methotrexate |
Reproductive: Human | |
| |
| Chen, J. and Roan, N.: Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts.
, Bio Protoc
5,
, 2015 | |
| Cells: | Endometrial epithelial and stromal fibroblasts |
| Enzyme(s) | Collagenase Type 1: 0.32% Hyaluronidase: 62 u/ml |
| Medium | DMEM |
Reproductive: Human | |
| |
| Evans, J. and Salamonsen, L.: Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade.
, Biol Reprod 90,
14, 2014 | |
| Cells: | Endometrial stromal |
| Enzyme(s) | Collagenase Type 3: 8 u/ml Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F-12 |
Reproductive: Human | |
| |
| Patel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta.
, Stem Cells Transl Med 2,
839-47, 2013 | |
| Cells: | Endothelial colony forming |
| Enzyme(s) | Collagenase Type 1: 0.1% Deoxyribonuclease I: 0.1% Neutral Protease: 0.075% |
| Medium | HBSS |
Reproductive: Human | |
| |
| Patel, M., Ghosh, M., Fahey, J. and Wira, C.: Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol.
, PLoS ONE
7,
e35654, 2012 | |
| Cells: | Uterine epithelial |
| Enzyme(s) | Pancreatin: 0.34% Hyaluronidase: 0.01% Collagenase: 0.16% |
| Medium | HBSS |
Reproductive: Human | |
| |
| Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1,
48, 2012 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.4% Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F12 |
Reproductive: Human | |
| |
| Steigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta.
, Curr Protoc Stem Cell Biol
Chapter 1,
Unit 1E.2, 2007 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 10% Neutral Protease: see reference |
| Medium | CMF-DPBS |
Reproductive: Human | |
| |
| Lockwood, C., Arcuri, F., Toti, P., Felice, C., Krikun, G., Guller, S., Buchwalder, L. and Schatz, F.: Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis.
, Am J Pathol 169,
1294-302, 2006 | |
| Cells: | Decidual |
| Enzyme(s) | Collagenase: 0.25% Deoxyribonuclease I: 6.25 u/ml |
| Medium | DMEM/F12 |
Reproductive: Human | |
| |
| Spessotto, P., Bulla, R., Danussi, C., Radillo, O., Cervi, M., Monami, G., Bossi, F., Tedesco, F., Doliana, R. and Colombatti, A.: EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall.
, J Cell Sci
119,
4574, 2006 | |
| Cells: | Extravillanous trophoblasts and decidual stromal |
| Enzyme(s) | Trypsin: 0.1-0.25% Collagenase Type 1: 0.1% Deoxyribonuclease I: 0.02-0.05% |
| Medium | HBSS RPMI |
Reproductive: Human | |
| |
| Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun: Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro
, Chin Med J (Engl)
118,
1857-62, 2005 | |
| Cells: | Sertoli cells |
| Enzyme(s) | Trypsin: 2.5% Collagenase Type 1: 2% Hyaluronidase: 1% |
| Medium | DMEM/F-12 |
Reproductive: Human | |
| |
| Meter, R., Wira, C. and Fahey, J.: Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture.
, Fertil Steril
84,
191, 2005 | |
| Cells: | Uterine epithelial |
| Enzyme(s) | Pancreatin: 0.34% Hyaluronidase: 0.01% Collagenase: 0.16% |
| Medium | HBSS |
Reproductive: Human | |
| |
| Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E: Clonogenicity of human endometrial epithelial and stromal cells
, Biol Reprod 70,
1738-50, 2004 | |
| Cells: | Endometrial epithelial and stromal cells |
| Enzyme(s) | Collagenase Type 3: 0.03% Deoxyribonuclease I: 0.004% |
| Medium | DMEM/F-12 |
Reproductive: Human | |
| |
| Wang Xin, Athayde Neil, Trudinger Brian: Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease
, Am J Obstet Gynecol
190,
596-601, 2004 | |
| Cells: | Endothelial placental |
| Enzyme(s) | Collagenase Type 1: 0.2% Trypsin: 0.2% Deoxyribonuclease I: 0.1% |
| Medium | DMEM |
Reproductive: Human | |
| |
| Sun Kang, Myatt Leslie: Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts
, Endocrinology 144,
5568-77, 2003 | |
| Cells: | Amnion epithelial and fibroblast |
| Enzyme(s) | Trypsin: 0.125% Collagenase: 0.1% Deoxyribonuclease I: 0.02% |
| Medium | PBS |
Reproductive: Human | |
| |
| Oberlin, E., Tavian, M., Blazsek, I., and Peault, B.: Blood-forming potential of vascular endothelium in the human embryo
, Development
129,
4147, 2002 | |
| Cells: | Endothelial Hematopoietic Stromal |
| Enzyme(s) | Collagenase Type 1/2/4: 0.1% |
| Medium | DMEM |
Reproductive: Human | |
| |
| Fahey John V, Wira Charles R: Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture
, J Infect Dis 185,
1606-13, 2002 | |
| Cells: | Uterine epithelial cells |
| Enzyme(s) | Pancreatin: 0.34% Collagenase: 0.16% Hyaluronidase: 0.16% |
| Medium | HBSS |
Reproductive: Human | |
| |
| Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S: Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells
, Hum Reprod 17,
2832-8, 2002 | |
| Cells: | Mesothelial |
| Enzyme(s) | Collagenase Type 1: 0.1% Deoxyribonuclease I: 0.05% |
| Medium | Eagle's MEM |
Reproductive: Human | |
| |
| Zhang, S., Wernig, M., Duncan, I., Brustle, O., and Thomson, J.: In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells
, Nat Biotechnol
19,
1129, 2001 | |
| Cells: | Stem, embryonic |
| Enzyme(s) | Neutral Protease: 0.01% - 0.02% |
| Medium | DMEM |
Reproductive: Human | |
| |
| Arnold, J., Kaufman, D., Seppala, M., and Lessey, B.: Endometrial stromal cells regulate epithelial cell grownth in vitro: a new co-culture model
, Hum Reprod 16,
836, 2001 | |
| Cells: | Endometrium epithelial and stromal cells |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Reproductive: Human | |
| |
| Yusuf RZ, Naeem R: Cytogenetic studies of spontaneous miscarriages: a seven year study to compare significance of primary vs. secondary culture methods for assessment of fetal karyotype yield and maternal cell contamination
, Early Pregnancy 5,
121-31, 2001 | |
| Cells: | Chorionic villi |
| Enzyme(s) | Trypsin: see reference Collagenase Type 3: 100 u/ml |
| Medium | HBSS |
Reproductive: Human | |
| |
| Nikitenko LL, MacKenzie IZ,Rees MC, Bicknell R: Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium
, Mol Hum Reprod 6,
811, 2000 | |
| Cells: | Endometrial endothelial cells |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | McCoys medium |
Reproductive: Human | |
| |
| Gargett CE, Bucak K, Rogers PA: Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells
, Hum Reprod 15,
293-301, 2000 | |
| Cells: | Microvascular endothelial cells |
| Enzyme(s) | Collagenase Type 2: 0.2% Deoxyribonuclease I: 0.0015% Trypsin: 0.05% |
| Medium | PBS |
Reproductive: Human | |
| |
| Zhang J, Lathbury LJ, Salamonsen LA: Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium
, Biol Reprod 62,
404-11, 2000 | |
| Cells: | Endometrial epithelial cells |
| Enzyme(s) | Collagenase Type 3: 45 u/ml Deoxyribonuclease I: .00035% |
| Medium | DMEM/F-12 |
Reproductive: Human | |
| |
| Runesson E, Ivarsson K, Janson P, Brannstrom M: Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle
, J Clin Endocrinol Metab 85,
4387-95, 2000 | |
| Cells: | Theca cells |
| Enzyme(s) | Collagenase Type 1: 0.3% Deoxyribonuclease I: 0.0005% Hyaluronidase: 0.1% |
| Medium | PBS |
Reproductive: Human | |
| |
| Friden BE, Runesson E, Hahlin M, Brannstrom M: Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum
, Mol Hum Reprod 6,
397-403, 2000 | |
| Cells: | Corpus luteum cells |
| Enzyme(s) | Collagenase Type 2: 0.25% Deoxyribonuclease I: .005% |
| Medium | PBS |
Reproductive: Human | |
| |
| Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M: Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture
, Mol Hum Reprod 5,
714-9, 1999 | |
| Cells: | Luteal cells |
| Enzyme(s) | Collagenase Type 2: 0.25% Deoxyribonuclease I: 0.005% |
| Medium | PBS |
Reproductive: Human | |
| |
| Hovatta O, Wright C, Krausz T, Hardy K, Winston RM: Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation
, Hum Reprod 14,
2519-24, 1999 | |
| Cells: | Follicles |
| Enzyme(s) | Collagenase Type 2: 0.025-0.1% |
| Medium | EBSS |
Reproductive: Human | |
| |
| Bradbury., R., Sunn, K., Crossley, M., Bai, M., Brown, E., Delbridge, D., and Conigrave, A.: Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta
, J Endocrinol 156,
425-430, 1998 | |
| Cells: | Placental |
| Enzyme(s) | Deoxyribonuclease I: 0.04% Collagenase Type 2: 0.1% |
| Medium | PSS |
Reproductive: Human | |
| |
| Huang JC, Liu DY, Dawood MY: The expression of vascular endothelial growth factor isoforms in cultured human endometrial stromal cells and its regulation by 17beta-oestradiol
, Mol Hum Reprod 4,
603-7, 1998 | |
| Cells: | Endometrial stromal cells |
| Enzyme(s) | Collagenase: 4000 u/ml |
| Medium | DMEM/F-12 |
Reproductive: Human | |
| |
| Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA: Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation
, Biol Reprod 59,
693-703, 1998 | |
| Cells: | Stromal endometrial |
| Enzyme(s) | Collagenase Type 3: 45 u/ml Deoxyribonuclease I: .00035% |
| Medium | DMEM/Ham's F12 |
Reproductive: Human | |
| |
| Takeuchi, K., Maruyama, I., Yamamoto, S., Oki, T., Nagata, Y.: Isolation and Monolayer Culture of Human Fallopian Tube Epithelial Cells
, In Vitro Cell Dev Biol 27,
720, 1991 | |
| Cells: | Epithelial, fallopian tube |
| Enzyme(s) | Collagenase Type 1: 1% |
| Medium | Medium 199 |
Reproductive: Human | |
| |
| Branchaud, C.L., Goodyer, C.G., Guyda, H.J. and Lefebvre, Y.: A Serum-Free System for Culturing Human Placental Trophoblasts
, In Vitro Cell Dev Biol 26,
865, 1990 | |
| Cells: | Trophoblasts, placental |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | EBSS, CMF |
Reproductive: Human | |
| |
| Jie, Z., Fey, S., Hager, H., Hollsberg, P., Ebbesen, P., and Larsen, P.: Markers For Human Placental Trophoblasts in Two-Dimensional Gel Electrophoresis
, In Vitro Cell Dev Biol 26,
937, 1990 | |
| Cells: | Trophoblasts, placental |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS |
Reproductive: Human | |
| |
| Egan, D., Grzegorczyk, V., Tricarico, K., Rueter, A.H., Olleman, W., and Marcotte, P.: Human Placental Chorionic Renin: Production, Purification and Characterization
, Biochim Biophys Acta 965,
68, 1988 | |
| Cells: | Chorionic, placental |
| Enzyme(s) | Deoxyribonuclease I: 0.003% |
| Medium | HBSS |
Reproductive: Human | |
| |
| Rinehart, C., Lyn-Cook, B., and Kaufman, D.: Gland formation from human endometrial epithelial cells in vitro
, In Vitro Cell Dev Biol 24 (10),
1037, 1988 | |
| Cells: | Endometrial |
| Enzyme(s) | Collagenase: 2% |
| Medium | RPMI 1640 |
Reproductive: Human | |
| |
| Morrish, D., and Siy, O.: Critical Factors in Establishing Monolayer Cultures of Normal Human Placental Cells in Serum-Free Medium
, Endocr Res 12 (3),
229, 1986 | |
| Cells: | Placental |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Reproductive: Human | |
| |
| Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture
, In Vitro 20,
743, 1984 | |
| Cells: | Epithelial Ovary |
| Enzyme(s) | Trypsin: 0.125% |
| Medium | HBSS, CMF |
Reproductive: Human | |
| |
| Siegfried, J., Nelson, K., Martin, J., and Kaufman, D.: Histochemical Identification of Cultured Cells From Human Endometrium
, In Vitro 20 (1),
25, 1984 | |
| Cells: | Epithelial Stromal |
| Enzyme(s) | Collagenase: 0.25% |
| Medium | See reference |
Reproductive: Human | |
| |
| Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues
, Endocr Res 10,
151, 1984 | |
| Cells: | Interna & corpus luteum Endometrium Ovarian Uterine |
| Enzyme(s) | Pronase: 0.1% |
| Medium | Moscona's BSS |
Reproductive: Human | |
| |
| Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures
, Methods Cell Biol 21,
195, 1980 | |
| Cells: | Epithelial Prostate |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS |
Reproductive: Human | |
| |
| Rifas, L., Fant, J., Makman, M., and Seifter, S.: The Characterization of Human Uterine Smooth Muscle Cells in Culture
, Cell Tissue Res 196,
385, 1979 | |
| Cells: | Smooth muscle, uterine |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | EBSS |
Reproductive: Human | |
| |
| Kirk, D., King, R., Heyes, J., Peachey, L., Hirsch, P., and Taylor, W.: Normal Human Edometrium in Cell Culture
, In Vitro 14 (8),
651, 1978 | |
| Cells: | Epithelial Stromal |
| Enzyme(s) | Collagenase Type 1: 180 u/ml |
| Medium | DMEM |
Reproductive: Insect | |
| |
| Ma, X., Wang, S., Do, T., Song, X., Inaba, M., Nishimoto, Y., Liu, L., Gao, Y., Mao, Y., Li, H., McDowell, W., Park, J., Malanowski, K., Peak, A., Perera, A., Li, H. et al.: Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary.
, PLoS ONE
9,
e90267, 2014 | |
| Cells: | Ovarian |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | Grace's |
Reproductive: Insect | |
| |
| Salmand, P. Iche-Torres, M. and Perrin, L.: Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis.
,Fly (Austin) Vol. 5,, ,
261, | |
| Cells: | Cardiac differentiating |
| Enzyme(s) | Collagenase Type 1: 20 u/ml Trypsin: 0.25% Deoxyribonuclease I: 4 u/ml |
| Medium | Schneider |
Reproductive: Monkey | |
| |
| Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB and Stouffer, RL: Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin.
, Hum Reprod 26,
1061-72, 2011 | |
| Cells: | Follicle |
| Enzyme(s) | Collagenase Type 1: 275 u/m Deoxyribonuclease I: 585 u/ml |
| Medium | HEPES |
Reproductive: Mouse | |
| |
| Hou, M., Han, J., Li, G., Kwon, M., Jiang, J., Emani, S., Taglauer, E., Park, J., Choi, E., Vodnala, M., Fong, Y., Emani, S., Rosas, I., Perrella, M. and Liu, X.: Multipotency of Mouse Trophoblast Stem Cells
, Stem Cell Res Ther 11,
55, 2020 | |
| Cells: | Trophoblast |
| Enzyme(s) | Collagenase Type 1: 0.1% Collagenase Type 2: 0.25% |
| Medium | DMEM |
Reproductive: Mouse | |
| |
| Lee, A, Rusch, J., Lima, A., Usmani, A., Huang, N., Lepamets, M., Vigh-Conrad, K., Worthington, R., et al: Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility.
, Nat Commun
10,
4626, 2019 | |
| Cells: | Testes |
| Enzyme(s) | Collagenase Type 1: 120 u/ml Deoxyribonuclease I: 0.1% Trypsin: 5% |
| Medium | MEM |
Reproductive: Mouse | |
| |
| Kang, H., Chen, L., Lichti-Kaiser, K., Liao, G., Gerrish, K., Bortner, C., Yao, Hu., Eddy, E. and Jetten, A.: Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis.
, Stem Cells 34,
2772-2783, 2016 | |
| Cells: | Testicular |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.1% |
| Medium | HBSS |
Reproductive: Mouse | |
| |
| Martin, L. and Seandel, M.: Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines.
, J Vis Exp
72,
e50017, 2013 | |
| Cells: | Spermatogonial stem |
| Enzyme(s) | Trypsin: 0.05% Collagenase Type 1: 0.03% Deoxyribonuclease I: 80 u/ml |
| Medium | DMEM |
Reproductive: Mouse | |
| |
| White, Y., Woods, D., Takai, Y., Ishihara, O., Seki, H. and Tilly, J.: Oocyte Formation by Mitotically Active Germ Cells Purified from Ovaries of Reproductive-Age Aomen.
, Nat Med 18,
413, 2012 | |
| Cells: | Ovarian |
| Enzyme(s) | Collagenase Type 4: 800 u/ml |
| Medium | HBSS |
Reproductive: Mouse | |
| |
| Jiang, J. and Kelly, K.: Isolation of Lymphocytes from Mouse Genital Tract Mucosa.
, J Vis Exp
67,
e4391, 2012 | |
| Cells: | Lymphocytes |
| Enzyme(s) | Collagenase: 450 u/ml |
| Medium | RPMI |
Reproductive: Mouse | |
| |
| Venkataraman, L., Lenis, A., Couri, B., Damaser, M. and Ramarurthi, A: Induced Regenerative Elastic Matrix Repair in LOXL 1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse, J Tissue Sci Eng 3,
3, 2012 | |
| Cells: | Vaginal smooth muscle |
| Enzyme(s) | Collagenase Type 2: 175 u/ml Elastase: 0.025% |
| Medium | DMEM/F12 |
Reproductive: Mouse | |
| |
| Getun, I., Torres, B. and Bois, P.: Flow Cytometry Purification of Mouse Meiotic Cells.
, J Vis Exp
50,
2602, 2011 | |
| Cells: | Testis, meiotic |
| Enzyme(s) | Collagenase Type 1: 120 u/ml Deoxyribonuclease I: 0.001% Trypsin: 0.1% |
| Medium | Gey's BSS |
Reproductive: Mouse | |
| |
| Yamatoya, K., Ito, C., Araki, M., Furuse, R. and Toshimori, K.: One-Step Collagenase Method for Zona Pellucida Removal in Unfertilized Eggs: Easy and Gentle Method for Large-Scale Preparation.
, Reprod Med Bio 10,
97-103, 2011 | |
| Cells: | Zona pellucid free eggs |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | THY-HEPES |
Reproductive: Mouse | |
| |
| Chen, L., Belton, R. and Nowak, R.: Basigin-Mediated Gene Expression Changes in Mouse Uterine Stromal Cells During Implantation.
, Endocrinology 150,
966, 2009 | |
| Cells: | Uterine stomal |
| Enzyme(s) | Neutral Protease: 0.6% Pancreatin: 0.25% Collagenase Type 3: 0.05% |
| Medium | DMEM/F12 |
Reproductive: Mouse | |
| |
| Breault, D., Min, I., Carlone, D., Farilla, L., Ambruzs, D., Henderson, D., Algra, S., Montgomery, R., Wagers, A. and Hole, N.: Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells.
, Proc Natl Acad Sci U S A 105,
10420, 2008 | |
| Cells: | Germ cells |
| Enzyme(s) | Collagenase Type 1: 100 u/ml |
| Medium | HBSS |
Reproductive: Mouse | |
| |
| Gekas, C., Rhodes, K. and Mikkola, H.: Isolation and Analysis of Hematopoietic Stem Cells from the Placenta.
, J Vis Exp
16,
e742, 2008 | |
| Cells: | Hematopoietic stem cells |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | PBS |
Reproductive: Mouse | |
| |
| Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta: Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse
, Biol Reprod 69,
985-94, 2003 | |
| Cells: | Sertoli |
| Enzyme(s) | Collagenase Type 2: 500 u/ml Hyaluronidase: 0.1% Deoxyribonuclease I: 0.0005% |
| Medium | DMEM |
Reproductive: Mouse | |
| |
| O'Shaughnessy PJ, Fleming LM, Jackson G, Hochgeschwender U, Reed P, Baker PJ: Adrenocorticotropic hormone directly stimulates testosterone production by the fetal and neonatal mouse testis
, Endocrinology 144,
3279-84, 2003 | |
| Cells: | Testicular cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F-12 |
Reproductive: Mouse | |
| |
| Faldikova, L., Diblikova, I., Canderle, J., Zraly, Z., Veznik, Z., and Sulcova, A.: Effects of Nutrition, Social Factors and Chronic Stress on the Mouse Leydig Cell Testosterone Production, Vet Med 46 (6),
160, 2001 | |
| Cells: | Leydig |
| Enzyme(s) | Collagenase: 0.06% |
| Medium | Medium E 199 |
Reproductive: Mouse | |
| |
| Lin, Q., Sirotkin, A., and Skoultchi, A.: Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t
, Mol Cell Biol
20 (6),
2122, 2000 | |
| Cells: | Seminiferous tubules |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Reproductive: Mouse | |
| |
| Eppig JJ, O'Brien MJ: Development in vitro of mouse oocytes from primordial follicles
, Biol Reprod 54,
197-207, 1996 | |
| Cells: | Oocyte-granulosa |
| Enzyme(s) | Collagenase Type 1: 0.1% Deoxyribonuclease I: 0.02% |
| Medium | Waymouth |
Reproductive: Mouse | |
| |
| Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes
, In Vitro Cell Dev Biol 27A,
713, 1991 | |
| Cells: | Uterine |
| Enzyme(s) | Trypsin: 0.2% Trypsin: 0.2% |
| Medium | HBSS |
Reproductive: Mouse | |
| |
| Tsai, P., Uchima, F., Hamamoto, S., and Bern, H.: Proliferation and Differentiation of Prepubertal Mouse Vaginal Epithelial Cells In Vitro and the Specificity of Estrogen-Induced Growth Retardation
, In Vitro Cell Dev Biol 27A,
461, 1991 | |
| Cells: | vaginal epithelial |
| Enzyme(s) | Collagenase Type 3: 38 u/ml |
| Medium | DMEM |
Reproductive: Mouse | |
| |
| Spindle, A.: In vitro Development of One-Cell Embryos from Outbred Mice: Influence of Culture Medium Composition
, In Vitro Cell Dev Biol 26,
151, 1990 | |
| Cells: | Cumulus, one-cell embryos |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | PBS, CMF |
Reproductive: Mouse | |
| |
| Turner, T., Bern, H., Young,P., and Cunha, G.: Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel
, In Vitro Cell Dev Biol 26,
722, 1990 | |
| Cells: | Epithelial, prostate gland |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Medium 199 |
Reproductive: Mouse | |
| |
| Thompson, T.C., Southgate, J., Kitchener, G., and Land, H.: Multistage Carcinogenesis Induced by ras and myc Oncogenes in a Reconstituted Organ
, Cell
56,
917, 1989 | |
| Cells: | Prostate |
| Enzyme(s) | Trypsin: 1.0% |
| Medium | HBSS/ DMEM |
Reproductive: Mouse | |
| |
| Tomooka, Y., DiAugustine, R., and McLachlan, J.: Proliferation of Mouse Uterine Epithelial Cells in Vitro
, Endocrinology 118 (3),
1011, 1986 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | Medium 199 |
Reproductive: Mouse | |
| |
| Bigsby, R., Cooke, P., and Cunha, G.: A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells
, Am J Physiol 251,
E630, 1986 | |
| Cells: | Epithelial Mesencymal |
| Enzyme(s) | Trypsin: 1% |
| Medium | DMEM |
Reproductive: Mouse | |
| |
| Cooke, P., Uchima, F., Fujii, D., Bern, H., and Cunha, G.: Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma
, Proc Natl Acad Sci U S A 83,
2109, 1986 | |
| Cells: | Epithelial |
| Enzyme(s) | Trypsin: 1% |
| Medium | Medium 199 |
Reproductive: Mouse | |
| |
| Stalvcey, J. and Payne, A.: Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains
, Endocrinology 112,
1696, 1983 | |
| Cells: | Leydig Testis |
| Enzyme(s) | Deoxyribonuclease I: 0.001% |
| Medium | Medium 199 w/ BSA |
Reproductive: Mouse | |
| |
| Iguchi, T., Uchima, F.A., Ostrander, P., and Bern, H.: Growth of Normal Mouse Vaginal Epithelial Cells in and on Collagen Gels
, Proc Natl Acad Sci U S A 80,
3743, 1983 | |
| Cells: | Epithelial, vagina |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | HBSS |
Reproductive: Ovine | |
| |
| Letouzey, V., Tan, K., Deane, J., Ulrich, D., Gurung, S., Ong, Y. and Gargett, C.: Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium.
, PLoS ONE
10,
e0127531, 2015 | |
| Cells: | Endometrial stromal |
| Enzyme(s) | Collagenase Type 1: 0.5% Deoxyribonuclease I: 0.04 u/ml |
| Medium | DMEM/F-12 |
Reproductive: Ovine | |
| |
| Salamonsen, L., Sum O, W., Doughton, B., and Findlay, J.: The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium
, Endocrinology 117 (5),
2148, 1985 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 125 - 190 u/ml |
| Medium | DMEM |
Reproductive: Porcine | |
| |
| Park, M., Park, J., Kim, M., Lee, K., Park, H., Yun, J., Choi, J., Lee, E. and Lee, S.: Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes.
, J Assist Reprod Genet
31,
983-91, 2014 | |
| Cells: | Testicular |
| Enzyme(s) | Collagenase Type 4: 0.1% Hyaluronidase: 0.1% Trypsin: 0.25% |
| Medium | DMEM |
Reproductive: Porcine | |
| |
| Dirami G, Ravindranath N, Pursel V, Dym M: Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM
, Biol Reprod 61,
225-30, 1999 | |
| Cells: | Seminiferous epithelial cells |
| Enzyme(s) | Collagenase: 0.15% Deoxyribonuclease I: .0001% Hyaluronidase: 0.15% Trypsin: 0.05% |
| Medium | DMEM/F12 |
Reproductive: Porcine | |
| |
| Ciereszko, R., Petroff, B., Ottobre, A., Guan, Z., Stokes, B., and Ottobre, J.: Assessment of the Mechanism by Which Prolactin Stimulates Progesterone Production by Early Corpora Lutea of Pigs
, J Endocrinol 159,
201, 1998 | |
| Cells: | Corpus Leuteum |
| Enzyme(s) | Collagenase Type 4: 600 u/ml |
| Medium | Medium 199 |
Reproductive: Porcine | |
| |
| Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues
, Endocr Res 10,
151, 1984 | |
| Cells: | Interna & corpus luteum Endometrium Ovarian Uterine |
| Enzyme(s) | Pronase: 0.1% |
| Medium | Moscona's BSS |
Reproductive: Porcine | |
| |
| Mather, J., Saez, J., and Haour, F.: Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells
, Endocrinology 110,
933, 1982 | |
| Cells: | Leydig Testis |
| Enzyme(s) | Trypsin: 0.0003% |
| Medium | Lebovitz L-15 Medium |
Reproductive: Rabbit | |
| |
| Kubota, H., Wu, X., Goodyear, S., Avarbock, M. and Brinster, R.: Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties.
, FASEB J
25,
2604, 2011 | |
| Cells: | Testicular germ |
| Enzyme(s) | Collagenase Type 1: 0.1% Trypsin: 0.25% Deoxyribonuclease I: 0.7% |
| Medium | HBSS |
Reproductive: Rabbit | |
| |
| Kubota, H., Wu, X., Goodyear, S., Avarbock, M. and Brinster, R.: Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties.
, FASEB J
25,
2604, 2011 | |
| Cells: | Testicular germ |
| Enzyme(s) | Collagenase Type 1: 0.1% Trypsin: 0.25% Deoxyribonuclease I: 0.7% |
| Medium | HBSS |
Reproductive: Rabbit | |
| |
| Setrakian, S., Oliveros-Saunders, B., and Nicosia, S.: Growth Stimulation of Ovarian and Extraovarian Mesothelial Cells by Corpus Luteum Extract
, In Vitro Cell Dev Biol 29A,
879, 1993 | |
| Cells: | Ovarian Mesothelial |
| Enzyme(s) | Collagenase Type 1: 300 u/ml |
| Medium | HBSS |
Reproductive: Rabbit | |
| |
| Phillippe, M., Saunders, T., and Bangalore, S.: Alpha-1,Alpha-2,and Beta Adrenergic Signal Transduction in Cultured Uterine Myocytes
, In Vitro Cell Dev Biol 26,
369, 1990 | |
| Cells: | Myocytes, uterine |
| Enzyme(s) | Deoxyribonuclease I: 200 µg/ml |
| Medium | HBSS-HEPES buffer |
Reproductive: Rabbit | |
| |
| Piquette, G., and Timms, B.: Isolation and Characterization of Rabbit Ovarian Surface Epithelium, Granulosa Cells, and Peritoneal Mesothelium in Primary Culture
, In Vitro Cell Dev Biol 26,
471, 1990 | |
| Cells: | Ovarian surface epithelial and peritoneal mesothelial |
| Enzyme(s) | Collagenase Type 1: 300 IU/ml (280 IU/mg) |
| Medium | HBSS, CMF |
Reproductive: Rabbit | |
| |
| Mulholland, J., Winterhager, E., and Beier, H.: Changes in Proteins Synthesized by Rabbit Endometrial Epithelial Cells Following Primary Culture
, Cell Tissue Res 252,
123, 1988 | |
| Cells: | Endometrial epithelial |
| Enzyme(s) | Collagenase Type 1: 0.005% |
| Medium | DMEM |
Reproductive: Rabbit | |
| |
| Boulet, A., and Fortier, M.: Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment
, In Vitro Cell Dev Biol 23,
93, 1987 | |
| Cells: | Myometrial |
| Enzyme(s) | Trypsin: 0.02%, 0.03%, 0.0375% |
| Medium | HBSS |
Reproductive: Rabbit | |
| |
| Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium)
, Int J Gynecol Pathol 3,
348, 1984 | |
| Cells: | Mesothelial and surface epithelial Ovaries |
| Enzyme(s) | Trypsin: 0.125%-0.5% |
| Medium | Medium 199 |
Reproductive: Rat | |
| |
| Jiang, H., Zhang, Y., Tian, K., Wang, B. and Han, S.: Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells.
, Sci Rep
7,
41837, 2017 | |
| Cells: | Placental mesenchymal |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.02% Neutral Protease: 0.1% |
| Medium | DMEM |
Reproductive: Rat | |
| |
| Sharma RS, Pal PC, Rajalakshmi M.: Isolation and Culture of Leydig Cells from Adult Rats, Indian J Clinical Biochem 21,
27, 2006 | |
| Cells: | Leydig cells |
| Enzyme(s) | Collagenase: .05-0.1% |
| Medium | Medium 199 |
Reproductive: Rat | |
| |
| Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization
, Biol Proced Online 3 (1),
35, 2001 | |
| Cells: | Seminiferous tubules |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Krebs-Ringer bicarbonate buffer (see reference) |
Reproductive: Rat | |
| |
| Ando, M., Kol, S., Irahara, M., Sirois, J., and Adashi, E.: Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process
, Mol Cell Endocrinol
157,
21, 1999 | |
| Cells: | Ovary |
| Enzyme(s) | Collagenase Type 1: 144 u/ml |
| Medium | McCoy's 5a |
Reproductive: Rat | |
| |
| Leckie, C., Welberg, L., and Seckl, J.: 11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells
, J Endocrinol 159,
233, 1998 | |
| Cells: | Testes |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Medium 199 w/ Hank's salts |
Reproductive: Rat | |
| |
| Tellieria, C., Ou, J., Sugino, N., Ferguson, S., and Gibori, G.: The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid
, Endocrinology 139 (8),
3597, 1998 | |
| Cells: | Corpus luteum |
| Enzyme(s) | Neutral Protease: 2.4 u/ml Deoxyribonuclease: 200u/ml |
| Medium | Serum-free medium (see reference) |
Reproductive: Rat | |
| |
| Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR: Effects of insulin and insulin-like growth factors on proliferation of rat ovarian theca-interstitial cells
, Biol Reprod 56,
891-7, 1997 | |
| Cells: | Ovarian theca-interstitial |
| Enzyme(s) | Collagenase Type 1: 0.5% Deoxyribonuclease I: 0.02% |
| Medium | Medium 199 |
Reproductive: Rat | |
| |
| Onoda, M. and Djakiew, D.: Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin
, In Vitro Cell Dev Biol 27,
215, 1991 | |
| Cells: | Sertoli |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | DMEM |
Reproductive: Rat | |
| |
| Abayasekara, D., Kurlak, L., Band, A., Sullivan, M., and Cooke, B.: Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis
, In Vitro Cell Dev Biol 27,
253, 1991 | |
| Cells: | Leydig |
| Enzyme(s) | Trypsin: 0.02% |
| Medium | DMEM |
Reproductive: Rat | |
| |
| Branham, W., Lyn-Cook, B., Andrews, A., McDaniel, M., Sheehan, D.: Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix
, In Vitro Cell Dev Biol 27,
442, 1991 | |
| Cells: | Uterine |
| Enzyme(s) | Trypsin: 1% |
| Medium | HBSS |
Reproductive: Rat | |
| |
| Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis
, In Vitro Cell Dev Biol 26,
24, 1990 | |
| Cells: | Leydig Adrenal |
| Enzyme(s) | Collagenase Type 2: 0.03% (adrenal) |
| Medium | Krebs Ringer bicarbonate buffer |
Reproductive: Rat | |
| |
| Pampfer, S., Vanderheyden, I., Michiels, B., and DeHertogh, R.: Co-Culture of Two-Cell Rat Embryos on Cell Monolayers
, In Vitro Cell Dev Biol 26,
944, 1990 | |
| Cells: | Uterine |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | PBS |
Reproductive: Rat | |
| |
| Hadley MA, Weeks BS, Kleinman HK, Dym M: Laminin promotes formation of cord-like structures by Sertoli cells in vitro
, Dev Biol 140,
318-27, 1990 | |
| Cells: | Sertoli cells |
| Enzyme(s) | Collagenase Type 2: 500 u/ml Deoxyribonuclease I: 0.0005% Hyaluronidase: 0.1% |
| Medium | PBS |
Reproductive: Rat | |
| |
| Rajan, V. and Menon, K.: Differential Uptake and Metabolism of Free and Esterified Cholesterol from High-density Lipoproteins in the Ovary
, Biochim Biophys Acta 959,
206, 1988 | |
| Cells: | Luteal, ovaries |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | McCoy's |
Reproductive: Rat | |
| |
| Skinner, M., Fetterolf, P., and Anthony, C.: Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function
, J Biol Chem 263,
2884, 1988 | |
| Cells: | Sertoli |
| Enzyme(s) | Trypsin: 0.15% |
| Medium | (see reference) |
Reproductive: Rat | |
| |
| Glasser, S., Julian, J., Decker, G., Tang, J., and Carson, D.: Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells
, J Cell Biol
107 (6),
2409, 1988 | |
| Cells: | Luminal epithelial |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | HBSS |
Reproductive: Rat | |
| |
| Conti, C., and Tasat, D.: Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone
, J Steroid Biochem 24 (3),
747, 1986 | |
| Cells: | Vaginal epithelial |
| Enzyme(s) | Trypsin: 0.5% |
| Medium | PBS |
Reproductive: Rat | |
| |
| Rajan, V. and Menon, K.: Involvement of Microtubules in Lipoprotein Degradation and Utilization for Steroidogenesis in Cultured Rat Luteal Cells
, Endocrinology 117,
2408, 1985 | |
| Cells: | Luteal, ovaries |
| Enzyme(s) | Deoxyribonuclease I: 0.0004% |
| Medium | McCoy's |
Reproductive: Rat | |
| |
| Hadley, M., Byers, S., Suarez-Quian, C., Kleinman, H., and Dym, M.: Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro
, J Cell Biol
101,
1511, 1985 | |
| Cells: | Sertoli |
| Enzyme(s) | Trypsin: 0.025% |
| Medium | DMEM |
Reproductive: Rat | |
| |
| Kassis, J., Walent, J., and Gorski, J.: Estrogen Receptors in Rat Uterine Cell Cultures: Effects of Medium on Receptor Concentration
, Endocrinology 115,
762, 1984 | |
| Cells: | Uterine |
| Enzyme(s) | Deoxyribonuclease I: 0.025% |
| Medium | DMEM buffered with HEPES |
Reproductive: Rat | |
| |
| Hsueh, A., Bambino, T., Zhuang, L., Welsh, T., and Ling, N.: Mechanism of the Direct Action of Gonadotropin Releasing Hormone and Its Antagonist on Androgen Biosynthesis by Cultured Rat Testicular Cells
, Endocrinology 112,
1653, 1983 | |
| Cells: | Testicular |
| Enzyme(s) | Deoxyribonuclease I: 10 µg/ml |
| Medium | HEPES |
Reproductive: Rat | |
| |
| Rajendran, K., Hwang, J., and Menon, K.: Binding, Degradation an Utilization of Plasma High Density and Low Density Lipoproteins for Progesterone Production In Cultured Rat Luteal Cells
, Endocrinology 112,
1746, 1983 | |
| Cells: | Luteal, ovaries |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | EBSS |
Reproductive: Rat | |
| |
| Rich, K., Bardin, C., Gunsalus, G., and Mather, J.: Age-Dependent Pattern of Androgen-Binding Protein Secretion from Rat Sertoli Cells in Primary Culture
, Endocrinology 113,
2284, 1983 | |
| Cells: | Sertolli, seminiforous tubules |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | Serum-free medium |
Reproductive: Rat | |
| |
| Azhar, S. and Reaven, E.: Effect of Antimicrotubule Agents on Microtubules and Steroidogenesis in Luteal Cells
, Am J Physiol 243,
E380, 1982 | |
| Cells: | Luteal |
| Enzyme(s) | Deoxyribonuclease I: 0.0004% |
| Medium | Medium 199 |
Reproductive: Rat | |
| |
| Ramachandran, J., and Sairam, M.R.: The Effects Of Interstitial Cell-Stimulating Hormone, Its Subunits,and Recombinants on Isolated Rat Leydig Cells
, Arch Biochem Biophys
167,
294, 1975 | |
| Cells: | Leydig |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Krebs Ringer bicarbonate buffer |
Scales: Fish | |
| |
| Lo, S., Grabowski, S., Lynch, T., Kern, D., Taylor, J.T., and Chen, T.: Isolation of xanthophores from the goldfish
, In Vitro 18,
356, 1982 | |
| Cells: | Pigment, xanthopores |
| Enzyme(s) | Deoxyribonuclease I: 0.005% |
| Medium | Medium 199 w/BSA |
Skin: Canine | |
| |
| Zheng, Y., Nace, A., Chen, W., Watkins, K., Sergott, L., Homan, Y., Vandeberg, J., Breen, M. and Stenn, K.: Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis.
, Dev Dyn
239,
2619-26, 2010 | |
| Cells: | Epidermal |
| Enzyme(s) | Collagenase: 0.35% Neutral Protease: 0.1% |
| Medium | DMEM |
Skin: Frog | |
| |
| Nishikawa, A., Shimizu-Nishikawa, K., and Miler, L.: Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis
, In Vitro Cell Dev Biol 26,
1128, 1990 | |
| Cells: | Epidermal |
| Enzyme(s) | Trypsin: 0.18% |
| Medium | Barth's solution, CMF |
Skin: Goat | |
| |
| Deng, Y., Hu, J. and Athanasiou, K.: Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential.
, Arthritis Rheum 56,
168, 2007 | |
| Cells: | Dermis isolated aggrecan sensitive |
| Enzyme(s) | Neutral Protease: 0.5% Collagenase Type 2: 200 u/ml |
| Medium | DMEM |
Skin: Human | |
| |
| Tan, J., Jaafar, F. and Makpol, S.: Proteomic Profiling of Senescent Human Diploid Fibroblasts Treated with gamma-tocotrienol.
, BMC Complement Altern Med
18,
314, 2018 | |
| Cells: | Diploid fibroblasts |
| Enzyme(s) | Collagenase Type 1: 0.03% |
| Medium | DMEM |
Skin: Human | |
| |
| Chen, Z., Wang, X., Jin, T., Wang, Y., Hong, C., Tan, L., Dai, T., Wu, L., Zhuang, Z. and Shi, C.: Increase in the Radioresistance of Normal Skin Fibroblasts but not Tumor Cells by Mechanical Injury.
, Cell Death Dis
8,
e2573, 2017 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Neutral Protease: 0.1% Collagenase Type 1: 0.25% |
| Medium | DMEM |
Skin: Human | |
| |
| Botting, Ra., Bertram, K., Baharlou, H., Sandgren, K., Fletcher, J., Rhodes, J., Rana, H., Plasto, T., Wang, X., Lim, J., Barnouti, L., Kohout, M., Papadopoulos, T., Merten, S., Olbourne, N. and Cunningham, A.: Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes.
, J Leukoc Biol 101,
1393-1403, 2017 | |
| Cells: | Mononuclear phagocytes |
| Enzyme(s) | Neutral Protease: 1 u/ml Deoxyribonuclease I: 50 u/ml Collagenase: 200 u/ml Trypsin: 0.5% |
| Medium | RPMI-1640 |
Skin: Human | |
| |
| Cerny, D., Haniffa, M., Shin, A., Bigliardi, P., Tan, B., Lee, B., Poidinger, M., Tan, E., Ginhoux, F. and Fink, K.: Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection.
, PLoS Pathog 10,
e1004548, 2014 | |
| Cells: | Skin |
| Enzyme(s) | Collagenase Type 4: 0.08-0.2% Neutral Protease: 0.1% Deoxyribonuclease I: 0.005% |
| Medium | RPMI |
Skin: Human | |
| |
| Cheuk, S., Wiken, M., Blomqvist, L., Nylen, S., Talme, T., Stahle, M.and Eidsmo, L.: Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis.
, J Immunol 192,
3111, 2014 | |
| Cells: | Skin |
| Enzyme(s) | Neutral Protease: 5 u/ml Collagenase Type 3: 0.3% Deoxyribonuclease I: 0.005% |
| Medium | RPMI 1640 |
Skin: Human | |
| |
| Douvaras, P., Wang, J., Zimmer, M., Hanchuk, S., O'Bara, M., Sadiq, S., Sim, F., Goldman, J. and Fossati, V.: Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells.
, Stem Cell Reports
3,
250, 2014 | |
| Cells: | Skin fibroblasts |
| Enzyme(s) | Collagenase Type 1: 1000 u/ml |
| Medium | DMEM |
Skin: Human | |
| |
| Nagel, S., Rohr, F., Weber, C., Kier, J., Siemers, F., Kruse, C., Danner, S., Brandenburger, M. and Matthiessen, A.: Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro.
, PLoS ONE
8,
e78365, 2013 | |
| Cells: | Epidermal stem cells |
| Enzyme(s) | Neutral Protease: 2 u/ml Trypsin: 2.5% |
| Medium | DMEM |
Skin: Human | |
| |
| Karumbayaram, S., Lee, P., Azghadi, S., Cooper, A., Patterson, M., Kohn, D., Pyle, A., Clark, A., Byrne, J., Zack, J., Plath, K. and Lowry, W.: From Skin Biopsy to Neurons Through a Pluripotent Intermediate Under Good Manufacturing Practice Protocols, Stem Cells Transl Med 1,
36, 2012 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Collagenase animal free: 2% |
| Medium | HBSS |
Skin: Human | |
| |
| Huschtscha, L., Napier, C., Noble, J., Bower, K., Au, A., Campbell, H., Braithwaite, A. and Reddel, R.: Enhanced Isolation of Fibroblasts From Human Skin Explants.
, Biotechniques
53,
239, 2012 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | DMEM |
Skin: Human | |
| |
| Carriel, V., Garzon, I., Jimenez, J., Oliveira, A., Arias-Santiago, S., Campos, A., Sanchez-Quevedo, M. and Alaminos, M.: Epithelial and Stromal Developmental Patterns in a Novel Substitute of the Human Skin Generated with Fibrin-Agarose Biomaterials.
, Cells Tissues Organs
196,
1-12, 2012 | |
| Cells: | Fibroblasts and keratinocytes |
| Enzyme(s) | Neutral Protease: 0.5% Collagenase Type 1: 0.2% |
| Medium | DMEM |
Skin: Human | |
| |
| Zheng, Y., Nace, A., Chen, W., Watkins, K., Sergott, L., Homan, Y., Vandeberg, J., Breen, M. and Stenn, K.: Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis.
, Dev Dyn
239,
2619-26, 2010 | |
| Cells: | Epidermal |
| Enzyme(s) | Collagenase: 0.35% Neutral Protease: 0.1% |
| Medium | DMEM |
Skin: Human | |
| |
| Zhang, Q., Yamaza, T., Kelly, AP, Shi, S., Wang, S., Brown, J., Wang, L., French, S., Shi, S. and Le, A.: Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis.
, PLoS ONE
4,
e7798, 2009 | |
| Cells: | Skin and keloid progenitor |
| Enzyme(s) | Collagenase Type 1: 0.4% Neutral Protease: 0.3% |
| Medium | PBS |
Skin: Human | |
| |
| Tuan, T., Hwu, P., Ho, W., Yiu, P., Chang, R., Wysocki, A. and Benya, P.: Adenoviral Overexpression and Small Interfering RNA Suppression Demonstrate that Plasminogen Activator Inhibitor-1 Produces Elevated Collagen Accumulation in Normal and Keloid Fibroblasts.
, Am J Pathol 173,
1311-25, 2008 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Collagenase Type 1: 100-320 u/ml |
| Medium | DMEM |
Skin: Human | |
| |
| Chen, F., Zhang, W., Bi, D., Liu, W., Wei, X., Chen, F., Zhu, L., Cui, L. and Cao, Y.: Clonal Analysis of Nestin(-) Vimentin(+) Multipotent Fibroblasts Isolated from Human Dermis.
, J Cell Sci
120,
2875, 2007 | |
| Cells: | Dermal fibroblasts |
| Enzyme(s) | Neutral Protease: 0.1% Collagenase Type 1: 0.1% |
| Medium | DMEM |
Skin: Human | |
| |
| Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei: Isolation of a novel population of multipotent adult stem cells from human hair follicles
, Am J Pathol 168,
1879-88, 2006 | |
| Cells: | Hair follicular epithelial |
| Enzyme(s) | Neutral Protease: 1.25% Trypsin: 0.25% |
| Medium | DMEM/F-12 |
Skin: Human | |
| |
| Clark, R., Chong, B., Mirchandani, N., Yamanaka, K., Murphy, G., Dowgiert, R. and Kupper, T.: A Novel Method for the Isolation of Skin Resident T cells from Normal and Diseased Human Skin.
, J Invest Dermatol 126,
1059, 2006 | |
| Cells: | fibroblasts |
| Enzyme(s) | Collagenase: 0.5% Trypsin: 0.25% |
| Medium | DMEM/F12 |
Skin: Human | |
| |
| Li, A., Pouliot, N., Redvers, R., and Kaur, P.: Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny
, J Clin Invest 113,
390-400, 2004 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Neutral Protease: 0.4% Collagenase: 0.3% |
| Medium | DMEM |
Skin: Human | |
| |
| Babina Magda, Guhl Sven, Starke Andrae, Kirchhof Loreen, Zuberbier Torsten, Henz Beate M: Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming
, J Leukoc Biol 75,
244-52, 2004 | |
| Cells: | Mast cells |
| Enzyme(s) | Neutral Protease: 0.05% Collagenase Type 4: 1.0% |
| Medium | RPMI |
Skin: Human | |
| |
| Wang, H., Van Blitterswijk, C., Bertrand-De Haas, M., Schuurman, A. and Lamme, E.: Improved Enzymatic Isolation of Fibroblasts for the Creation of Autologous Skin Substitutes.
, In Vitro Cell Dev Biol Anim 40,
268, 2004 | |
| Cells: | Dermal fibroblasts |
| Enzyme(s) | Collagenase: 0.5-1.0% |
| Medium | DMEM |
Skin: Human | |
| |
| Tuan Tai-Lan, Wu Huayang, Huang Eunice Y, Chong Sheree S N, Laug Walter, Messadi Diana, Kelly Paul, Le Anh: Increased plasminogen activator inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures
, Am J Pathol 162,
1579-89, 2003 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Skin: Human | |
| |
| Supp Dorothy M, Wilson-Landy Kaila, Boyce Steven T: Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice
, FASEB J
16,
797-804, 2002 | |
| Cells: | Keratinocytes, fibroblasts, endothelial |
| Enzyme(s) | Neutral Protease: see reference Collagenase: see reference |
| Medium | see reference |
Skin: Human | |
| |
| Baudoux, B., Castanares-Zapatero, D., Leclercq-Smekens, M., Berna, N., and Poumay, Y.: The Tetraspanin CD9 Associates with the Integrin a6B4 in Cultured Human Epidermal Keratinocytes and is Involved in Cell Motility
, Eur J Cell Biol
79,
41, 2000 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Neutral Protease: 0.25% |
| Medium | PBS |
Skin: Human | |
| |
| Hybbinette S, Bostrom M, Lindberg K: Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation
, Exp Dermatol
8,
30-8, 1999 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Neutral Protease: 0.25% Trypsin: 0.17% Thermolysin: 0.05% |
| Medium | see reference |
Skin: Human | |
| |
| Grutzkau A, Kruger-Krasagakes S, Baumeister H, Schwarz C, Kogel H, Welker P, Lippert U, Henz BM, Moller A: Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206
, Mol Biol Cell
9,
875-84, 1998 | |
| Cells: | Human skin mast cells |
| Enzyme(s) | Neutral Protease: 0.1% Collagenase Type 1: see reference |
| Medium | PBS |
Skin: Human | |
| |
| Reece J C, Handley A J, Anstee E J, Morrison W A, Crowe S M, Cameron P U: HIV-1 selection by epidermal dendritic cells during transmission across human skin
, J Exp Med 187,
1623-31, 1998 | |
| Cells: | Epidermal |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 0.5-1.0% |
| Medium | PBS |
Skin: Human | |
| |
| Judd, D., Battista, P., and Behm, D.: Culture of Human Keratinocytes in Defined Serum-Free Medium, Focus 19 (1),
2, 1997 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Dulbecco's PBS |
Skin: Human | |
| |
| Harley, C., and Sherwood, S.: Methods in Molecular Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75,Pollard, J., and Walker, J., Humana Press,
23, 1997 | |
| Cells: | Epidermis plus dermis Abdomen or inner forearm |
| Enzyme(s) | Trypsin: 0.125% |
| Medium | DMEM, M199 |
Skin: Human | |
| |
| Regnier, M: Culture of Human Karatinocytes,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons Ltd,
11B:4.1, 1995 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Skin: Human | |
| |
| Hirel, B., Chesne, C., Pailgheret, J., and Guillouzo, A.: Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions
, In Vitro Cell Dev Biol 30A,
372, 1994 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS |
Skin: Human | |
| |
| Hansbrough, J., Cooper, M., Cohen, R., Spielvogel, R., Greenleaf, G., Bartel, R., and Naughton, G.: Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice
, Surgery 111 (1),
438, 1992 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Skin: Human | |
| |
| Bell, C. and Quinton, P.: Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells
, In Vitro Cell Dev Biol 27,
47, 1991 | |
| Cells: | Sweat duct |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | MEM |
Skin: Human | |
| |
| Limat, A., Hunziker, T., Boillat, C., Noser, F., and Wiesmann, U.: Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation
, In Vitro Cell Dev Biol 26,
709, 1990 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | CMF solution |
Skin: Human | |
| |
| Krouse, M., Hagiqara, G., Chen, J., Lewiston, N., and Wine, J.: Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells
, Am J Physiol 257,
C129, 1989 | |
| Cells: | Sweat gland |
| Enzyme(s) | Collagenase Type 2: 0.015% |
| Medium | (see reference) |
Skin: Human | |
| |
| Pedersen, P.: Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis
, In Vitro Cell Dev Biol 25 (4),
342, 1989 | |
| Cells: | Human sweat duct |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | RPMI 1640 (see reference) |
Skin: Human | |
| |
| Peacocke, M., Yaar, M., Mansur, C., Chao, M., and Gilchrest, B.: Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes
, Proc Natl Acad Sci U S A 85,
5282, 1988 | |
| Cells: | Melanocytes, skin/ foreskin |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | PBS |
Skin: Human | |
| |
| Lee, C., Carpenter, F., Coaker, T., and Kealey, T.: The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands
, J Cell Sci
83,
103, 1986 | |
| Cells: | Human sweat glands |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | HBSS |
Skin: Human | |
| |
| Dover, R. and Potten, C.: Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes
, J Invest Dermatol 80,
423, 1983 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM |
Skin: Human | |
| |
| McCoy, B., Galdun, J., and Cohen, I.: Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts
, In Vitro 18 (1),
79, 1982 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | HBSS |
Skin: Human | |
| |
| Davies, P. and Kerr, C.: Modification of LDL Metabolism by Growth Factors in Cultured Vascular Cells and Human Skin Fibroblasts
, Biochim Biophys Acta 712,
26, 1982 | |
| Cells: | Smooth muscle, fibroblasts |
| Enzyme(s) | Trypsin: 0.055% |
| Medium | DMEM |
Skin: Human | |
| |
| Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A: Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells
, J Cell Biol
94,
497-505, 1982 | |
| Cells: | Epidermal keratinocytes |
| Enzyme(s) | Trypsin: 0.25% Collagenase: 0.2% Deoxyribonuclease I: 0.001% |
| Medium | Eagle's MEM |
Skin: Human | |
| |
| Liu, S., Eaton, M., and Karasek, M.: Growth Characteristics of Human Epidermal Keratinocytes from Newborn Foreskin in Primary and Serial Cultures
, In Vitro 15 (10),
813, 1979 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 0.3% |
| Medium | DMEM |
Skin: Human | |
| |
| Rheinwald, J., and Green, H.: Serial Cultivation of Strains of Human Epidermal Kerotinocytes: The Formation of Keratinizing Colonies from Single Cells
, Cell
6,
331, 1975 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | (see reference) |
Skin: Mouse | |
| |
| Spidale, N., Malhotra, N., Frascoli, M., Sylvia, K., Miu, B., Freeman, C., Stadinski, B., Huseby, E. and Kang, J.: Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent
Spontaneous Atopic Dermatitis
, Elife
9,
5118, 2020 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Collagenase Type 4: 0.2% Deoxyribonuclease I: 0.005% Neutral Protease: 5 u/ml |
| Medium | DMEM |
Skin: Mouse | |
| |
| Blanco, S., Bandiera, R., Popis, M., Hussain, S., Lombard, P., Aleksic, J., Sajini, A., Tanna, H., Cortes-Garrido, R., Gkatza, N., Dietmann, S. and Frye, M.: Stem Cell Function and Stress Response are Controlled by Protein Synthesis.
, Nature
534,
335-40, 2016 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 0.12% Collagenase Type 2: 0.05% Collagenase Type 4: 0.05% Hyaluronidase: 0.1% |
| Medium | Low calcium containing media |
Skin: Mouse | |
| |
| Rinkevich, Y., Walmsley, G., Hu, M., Maan, Z., Newman, A., Drukker, M., Januszyk, M., Krampitz, G., Gurtner, G., Lorenz, H., Weissman, I. and Longaker, M.: Identification and Isolation of a Dermal Lineage with Intrinsic Fibrogenic Potential.
, Science 348,
aaa2151, 2015 | |
| Cells: | Dermal fibroblasts |
| Enzyme(s) | Elastase: 0.012% Collagenase Type 4: 0.4% |
| Medium | DMEM |
Skin: Mouse | |
| |
| Cerny, D., Haniffa, M., Shin, A., Bigliardi, P., Tan, B., Lee, B., Poidinger, M., Tan, E., Ginhoux, F. and Fink, K.: Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection.
, PLoS Pathog 10,
e1004548, 2014 | |
| Cells: | Skin |
| Enzyme(s) | Collagenase Type 4: 0.08-0.2% Neutral Protease: 0.1% Deoxyribonuclease I: 0.005% |
| Medium | RPMI |
Skin: Mouse | |
| |
| Gu, D., Fan, Q and Xie, J.: Cell Population Analyses During Skin Carcinogenesis.
, J Vis Exp
,
e50311, 2013 | |
| Cells: | Epidermal |
| Enzyme(s) | Neutral Protease: 0.5% Collagenase Type 4: 0.1% |
| Medium | DMEM |
Skin: Mouse | |
| |
| Jensen, U., Ghazizadeh, S. and Owens, D.: Isolation and Characterization of Cutaneous Epithelial Stem Cells.
, Methods Mol Biol 989,
61-9, 2013 | |
| Cells: | Epithelial stem |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 0.4% |
| Medium | HBSS |
Skin: Mouse | |
| |
| Antsiferova, M., Martin, C., Huber, M., Feyerabend, T., Forster, A., Hartmann, K., Rodewald, H., Hohl, D. and Werner, S.: Mast Cells are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis.
, J Immunol 191,
6147-55, 2013 | |
| Cells: | Mast |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | IMDM |
Skin: Mouse | |
| |
| Scheibye-Knudsen, M., Ramamoorthy, M., Sykora, P., Maynard, S., Lin, P., Minor, R., Wilson, D., Cooper, M., Spencer, R., de Cabo, R., Croteau, D. and Bohr, V.: Cockayne Syndrome Group B Protein Prevents the Accumulation of Damaged Mitochondria by Promoting Mitochondrial Autophagy.
, J Exp Med 209,
855, 2012 | |
| Cells: | Dermal fibroblasts |
| Enzyme(s) | Collagenase: 400 u/ml |
| Medium | AminoMAX II |
Skin: Mouse | |
| |
| King, I., Kroenke, M. and Segal, B.: GM-CSF-Dependent, CD103+ Dermal Dendritic Cells Play a Critical Role in Th Effector Cell Differentiation After Subcutaneous Immunization.
, J Exp Med 207,
953, 2010 | |
| Cells: | Epidermal and dermal |
| Enzyme(s) | Collagenase Type 4: 0.18% Collagenase/Dispase: 0.18% |
| Medium | PBS |
Skin: Mouse | |
| |
| Farina, G., York, M., Di Marzio, M., Collins, C., Meller, S., Homey, B., Rifkin, I., Marshak-Rothstein, A., Radstake, T. and Lafyatis, R.: Poly(I:C) Drives Type I IFN- and TGFβ-Mediated Inflammation and Dermal Fibrosis Simulating Altered Gene Expression in Systemic Sclerosis.
, J Invest Dermatol 130,
2583-93, 2010 | |
| Cells: | Dermal fibroblasts |
| Enzyme(s) | Collagenase Type 2: 0.04% Trypsin: 0.025% |
| Medium | DME |
Skin: Mouse | |
| |
| Eidsmo, L., Allan, R., Caminschi, I., Van Rooijen, N., Heath, W. and Carbone, F.: Differential Migration of Epidermal and Dermal Dendritic Cells During Skin Infection.
, J Immunol 182,
3165, 2009 | |
| Cells: | Dermal |
| Enzyme(s) | Collagenase Type 3: 0.3% Deoxyribonuclease I: 0.0005% |
| Medium | HBSS |
Skin: Mouse | |
| |
| Crigler Lauren, Kazhanie Amita, Yoon Tae-Jin, Zakhari Julia, Anders Joanna, Taylor Barbara, Virador VictoriaM: Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages
, FASEB J
21,
2050-63, 2007 | |
| Cells: | Dermal |
| Enzyme(s) | Collagenase: 0.35% Deoxyribonuclease I: see reference |
| Medium | PBS |
Skin: Mouse | |
| |
| Baxter Ruth M, Crowell Thomas P, McCrann Margaret E, Frew Erica M, Gardner Humphrey: Analysis of the tight skin (Tsk1/+) mouse as a model for testing antifibrotic agents
, Lab Invest 85,
1199-209, 2005 | |
| Cells: | Dermal fibroblasts |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 0.25% |
| Medium | DMEM |
Skin: Mouse | |
| |
| Cha, S., Talavera, D., Demir, E., Nath, A. and Sierra-Honigmann, M.: A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin.
, Microvasc Res 70,
198, 2005 | |
| Cells: | Microvascular endothelial |
| Enzyme(s) | Neutral Protease: 0.005% Collagenase Type 1: 4% |
| Medium | DMEM |
Skin: Mouse | |
| |
| Montanaro F, Liadaki K, Volinski J, Flint A, and Kunkel LM.: Skeletal muscle engraftment potential of adult mouse skin side population cells
, Proc Natl Acad Sci U S A 100,
9336, 2003 | |
| Cells: | Skin side population |
| Enzyme(s) | Collagenase Type 4: 0.2% Neutral Protease: 1.2 u/ml |
| Medium | PBS |
Skin: Mouse | |
| |
| Takanami-Ohnishi Yoko, Amano Shinya, Kimura Sadao, Asada Sachie, Utani Atsushi, Maruyama Masumi, Osada Hiroyuki, Tsunoda Hajime, Irukayama-Tomobe Yoko, Goto Katsutoshi, Karin Michael, Sudo Tatsuhiko, Kasuya Yoshitoshi: Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity
, J Biol Chem 277,
37896-903, 2002 | |
| Cells: | Ear epidermal |
| Enzyme(s) | Trypsin: 0.1% Collagenase: 0.2% |
| Medium | PBS |
Skin: Mouse | |
| |
| Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation
, Mol Biol Cell
10,
1569-79, 1999 | |
| Cells: | Fibroblasts, mesangial, smooth muscle |
| Enzyme(s) | Trypsin: 0.25% Collagenase: see reference Soybean Trypsin Inhibitor: 0.05% |
| Medium | DMEM |
Skin: Porcine | |
| |
| Ando, W., Kutcher, J., Krawetz, R., Sen, A., Nakamura, N., Frank, C. and Hart, D.: Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage.
, Cytotherapy
16,
776, 2014 | |
| Cells: | Synovial membrane and skin stem |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM |
Skin: Porcine | |
| |
| Regauer S, Compton C: Cultured porcine epithelial grafts: an improved method
, J Invest Dermatol 94,
230-4, 1990 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Neutral Protease: 0.25% |
| Medium | Dulbecco-Vogt MEM |
Skin: Rat | |
| |
| Sugihara, H and Toda, S: Primary Tissue Intact and Dissociated Cell Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
3A:2.1, 1995 | |
| Cells: | Dermal fibroblasts and keratinocytes |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Ham's F-12 |
Skin: Rat | |
| |
| Laurent, S., Mednieks, M., and Rosenfield, R.: Growth of Sebaceous Cells in Monolayer Culture
, In Vitro Cell Dev Biol 28,
83, 1992 | |
| Cells: | Sebaceous |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | DMEM with FBS PBS |
Skin: Rat | |
| |
| Acheson, A., Barker, P., Alderson, R., Miller, F., and Murphy, R.: Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF
, Neuron 7,
265, 1991 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Trypsin: 0.2% |
| Medium | HEPES buffered DMEM |
Skin: Rat | |
| |
| Vaughan, F., Gray, R., and Bernstein, I.: Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes
, In Vitro Cell Dev Biol 22 (3),
141, 1986 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 1% |
| Medium | EBSS |
Spleen: Mouse | |
| |
| Hou, M., Han, J., Li, G., Kwon, M., Jiang, J., Emani, S., Taglauer, E., Park, J., Choi, E., Vodnala, M., Fong, Y., Emani, S., Rosas, I., Perrella, M. and Liu, X.: Multipotency of Mouse Trophoblast Stem Cells
, Stem Cell Res Ther 11,
55, 2020 | |
| Cells: | Trophoblast |
| Enzyme(s) | Collagenase Type 1: 0.1% Collagenase Type 2: 0.25% |
| Medium | DMEM |
Spleen: Mouse | |
| |
| Villarreal, E., Hewgley, W., Lang, W., Morton, C., Mao, S., Wu, J. and Sandoval, J.: In-bag Enzymatic Splenic Digestion: A Novel Alternative to Manual Morcellation?
, J Surg Res 218,
209-216, 2017 | |
| Cells: | Speen |
| Enzyme(s) | Collagenase Type 5: 5% Hyaluronidase: 2.5% Elastase: 1.25% |
| Medium | HBSS |
Spleen: Mouse | |
| |
| Klebanoff, C., Spencer, S., Torabi-Parizi, P., Grainger, J., Roychoudhuri, R., Ji, Y., Sukumar, M., Muranski, P., Scott, C., Hall, J., Ferreyra, G., Leonardi, A., Borman, Z. et al.: Retinoic Acid Controls the Homeostasis of Pre-cDC-Derived Splenic and Intestinal Dendritic Cells.
, J Exp Med 210,
1961, 2013 | |
| Cells: | Splenic |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | PBS |
Spleen: Mouse | |
| |
| Yi, T. and Cyster, J.: EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture.
, Elife
2,
e00757, 2013 | |
| Cells: | Splenocytes |
| Enzyme(s) | Collagenase Type 2: 0.16% Deoxyribonuclease I: 0.002% |
| Medium | PBS |
Spleen: Mouse | |
| |
| Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands.
, Immunity
34,
590, 2011 | |
| Cells: | Spleen, bone marrow endothelial |
| Enzyme(s) | Collagenase Type 4: 0.3-1.0% Deoxyribonuclease I: 20 u/ml |
| Medium | PBS |
Spleen: Mouse | |
| |
| Abou Fakher, F., Rachinel, N., Klimczak, M., Louis, J. and Doyen, N.: TLR9-Dependent Activation of Dendritic Cells by DNA from Leishmania Major Favors Th1 Cell Development and the Resolution of Lesions.
, J Immunol 182,
1386, 2009 | |
| Cells: | Dendritic |
| Enzyme(s) | Collagenase Type 4: 0.05% |
| Medium | RPMI 1640 |
Spleen: Mouse | |
| |
| Flano, E., Jewell, N., Durbin, R. and Durbin, J.: Methods Used to Study Respiratory Virus Infection.
,Curr Protoc Cell Biol Vol. Chapter 26,, ,
Unit 26.3, 2009 | |
| Cells: | Dendritic |
| Enzyme(s) | Collagenase Type 1: 0.5% |
| Medium | HBSS |
Spleen: Mouse | |
| |
| Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells.
, Proc Natl Acad Sci U S A 104,
17022, 2007 | |
| Cells: | Dentritic |
| Enzyme(s) | Collagenase: 300 u/ml Deoxyribonuclease I: 0.002% |
| Medium | RPMI 1640 |
Spleen: Mouse | |
| |
| Mueller, S., Matloubian, M., Clemens, D, Sharpe, A., Freeman, G., Gangappa, S., Larsen, C. and Ahmed, R.: Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection.
, Proc Natl Acad Sci U S A 104,
15430, 2007 | |
| Cells: | Splenic |
| Enzyme(s) | Collagenase Type 2: 0.1% Deoxyribonuclease I: 0.1% |
| Medium | RPMI |
Spleen: Mouse | |
| |
| Kang, H., Liu, M. and Datta, S.: Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells
, J Immunol 178,
7849-58, 2007 | |
| Cells: | Splenic dendritic |
| Enzyme(s) | Collagenase: 0.1% Deoxyribonuclease I: 0.005% |
| Medium | DMEM |
Spleen: Mouse | |
| |
| Benedict Chris A, De Trez Carl, Schneider Kirsten, Ha Sukwon, Patterson Ginelle, Ware Carl F: Specific remodeling of splenic architecture by cytomegalovirus
, PLoS Pathog 2,
e16, 2006 | |
| Cells: | Splenic stromal |
| Enzyme(s) | Collagenase Type 3: 100-400 u/ml |
| Medium | HBSS |
Spleen: Mouse | |
| |
| Siragam, V., Crow, A., Brinc, D., Song, S., Freedman, J. and Lazarus, A.: Intravenous Immunoglobulin Ameliorates ITP via Activating Fc Gamma Receptors on Dendritic Cells.
, Nat Med 12,
688, 2006 | |
| Cells: | Leukocytes |
| Enzyme(s) | Collagenase Type 4: 43 u/ml |
| Medium | RPMI 1640 |
Spleen: Mouse | |
| |
| McLellan, A., Kapp, M., Eggert, A., Linden, C., Bommhardt, U., Brocker, E., Kammerer, U. and Kampgen, E.: Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression
, Blood 99,
2084-93, 2002 | |
| Cells: | Dentritic |
| Enzyme(s) | Collagenase Type 1: 0.1% Deoxyribonuclease I: 0.001% |
| Medium | RPMI-1640 |
Spleen: Mouse | |
| |
| Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells
, J Exp Med 196,
1415, 2002 | |
| Cells: | Dentritic |
| Enzyme(s) | Collagenase Type 3: 0.1% Deoxyribonuclease I: 325 u/ml |
| Medium | RPMI 1640 |
Spleen: Mouse | |
| |
| Stagg, AJ, Burke, F, Hill, S and Knight, S.: Isolation of Mouse Spleen Dendritic Cells.
, Methods Mol Med
64,
9-22, 2001 | |
| Cells: | Dentritic cells |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 20 ug/ml |
| Medium | HEPES/RPMI 1640 |
Spleen: Mouse | |
| |
| Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures
, Blood 96,
3029-39, 2000 | |
| Cells: | Dentritic |
| Enzyme(s) | Collagenase: 100 u/ml |
| Medium | HBSS |
Stem: Canine | |
| |
| Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts.
, Tissue Eng Part A
19,
1295-302, 2013 | |
| Cells: | Stromal vascular |
| Enzyme(s) | Collagenase: see reference |
| Medium | Media 199E |
Stem: Canine | |
| |
| Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells.
, PLoS ONE
7,
e44693, 2012 | |
| Cells: | Amniotic membrane MSC |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 0.2% |
| Medium | LG-DMEM |
Stem: Canine | |
| |
| Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force.
, J Surg Res 152,
157, 2009 | |
| Cells: | Adipose stem cells |
| Enzyme(s) | Collagenase: see reference |
| Medium | Media-199 |
Stem: Equine | |
| |
| Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow.
, Vet Surg
37,
713, 2008 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Stem: Equine | |
| |
| Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells.
, Vet Surg
36,
613, 2007 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Stem: Human | |
| |
| Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat.
, Curr Protoc Stem Cell Biol
48,
e68, 2019 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 4: 150-300 u/ml |
| Medium | RPMI-1640 |
Stem: Human | |
| |
| Yam, G., Teo, E., Setiawan, M., Lovatt, M., Yusoff, N., Fuest, M., Goh, B. and Mehta, J.: Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy.
, J Cell Mol Med
,
, 2018 | |
| Cells: | Postnatal periodontal ligament |
| Enzyme(s) | Collagenase Type 1: 0.01% |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| Matsumura, E., Tsuji, K., Komori, K., Koga, H., Sekiya, I., Muneta, T.: Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells.
, Cytotherapy
19,
181-193, 2017 | |
| Cells: | Synovial mesenchymal stem |
| Enzyme(s) | Collagenase: 0.3% |
| Medium | MEM |
Stem: Human | |
| |
| Oppliger, B., Joerger-Messerli, M., Simillion, C., Mueller, M., Surbek, D. and Schoeberlein, A.: Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact.
, Cytotherapy
19,
829-838, 2017 | |
| Cells: | Wharton jelly mesenchymal stromal |
| Enzyme(s) | Collagenase Type 2: 270 u/ml |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| de Witte, S., Lambert, E., Merino, A., Strini, T., Douben, H., O'Flynn, L., Elliman, S., de Klein, A., Newsome, P., Baan, C. and Hoogduijn, M.: Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion.
, Cytotherapy
19,
798-807, 2017 | |
| Cells: | Umbilical cord mesenchymal stromal |
| Enzyme(s) | Collagenase Type 1: 0.2% Hyaluronidase: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | MEM |
Stem: Human | |
| |
| Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome.
, Stem Cell Res Ther 8,
69, 2017 | |
| Cells: | Adipose derived mesenchymal stem |
| Enzyme(s) | Collagenase animal free: 0.1% |
| Medium | PBS |
Stem: Human | |
| |
| Randelli, P., Menon, A., Ragone, V., Creo P., Alfieri, M., Perucca, O., Banfi, G., Cabitza, P., Tettamanti, G. and Anastasia, L.: Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study.
, BMC Complement Altern Med
16,
293, 2016 | |
| Cells: | Tendon stem |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | MEM |
Stem: Human | |
| |
| Rigamonti, A., Repetti, G., Sun, C., Price, F., Reny, D., Rapino, F., Weisinger, K., Benkler, C., Peterson, Q., Davidow, L., Hansson, E. and Rubin, L.: Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System.
, Stem Cell Reports
6,
993-1008, 2016 | |
| Cells: | Motor neurons |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | EBSS |
Stem: Human | |
| |
| Lim, J., Razi, Z., Law, J., Nawi, A., Idrus, R. and Ng, M.: MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord.
, Cytotherapy
18,
1493-1502, 2016 | |
| Cells: | Wharton jelly mesenchymal stromal |
| Enzyme(s) | Collagenase Type 2: 0.6% |
| Medium | DMEM |
Stem: Human | |
| |
| Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells
, Cytotherapy
18,
371-83, 2016 | |
| Cells: | Adipose stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells.
, Cytotherapy
17,
359-68, 2015 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | PBS |
Stem: Human | |
| |
| Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage.
, BMC Med Genomics
8,
41, 2015 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | HBSS |
Stem: Human | |
| |
| Wu, Z., Wang, J., Dong, R., Wang, L., Fan, Z., Liu, D. and Wang, S.: Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells.
, Int J Clin Exp Med
8,
7220-30, 2015 | |
| Cells: | Dental mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Stem: Human | |
| |
| Charville, G., Cheung, T., Yoo, B., Santos, P., Lee, G., Shrager, J. and Rando, T.: Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells.
, Stem Cell Reports
5,
621, 2015 | |
| Cells: | Skeletal muscle stem |
| Enzyme(s) | Collagenase Type 2: 750 u/ml Neutral Protease: 2 u/ml |
| Medium | Ham's F10 |
Stem: Human | |
| |
| Kusuma, G., Menicanin, D., Gronthos, S., Manuelpillai, U., Abumaree, M., Pertile, M., Brennecke, S. and Kalionis, B.: Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua.
, PLoS ONE
10,
e0141246, 2015 | |
| Cells: | Decidua mesenchymal stem |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 1.0% Deoxyribonuclease I: 0.005% |
| Medium | PBS |
Stem: Human | |
| |
| Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord.
, J Vis Exp
,
e52732, 2015 | |
| Cells: | Spinal cord neural progenitor |
| Enzyme(s) | PDS kit: with modifications |
| Medium | EBSS |
Stem: Human | |
| |
| Lee, S., Park, B., Kim, J., Jekarl, D., Choi, H., Lee, S., Kim, M., Kim, Y. and Park, M.: The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells.
, Cytotherapy
17,
1723-31, 2015 | |
| Cells: | Wharton jelly mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.1% Trypsin NF 1:250: 0.25% |
| Medium | PBS |
Stem: Human | |
| |
| Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells.
, Cytotherapy
16,
915, 2014 | |
| Cells: | Adipose derived mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | see reference |
Stem: Human | |
| |
| Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities.
, Cytotherapy
16,
789, 2014 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 1: 0.15% |
| Medium | DMEM |
Stem: Human | |
| |
| Hang, H., Yu, Y., Wu, N., Huang, Q., Xia, Q. and Bian, J.: Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction.
, PLoS ONE
9,
e104133, 2014 | |
| Cells: | Umbilical mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.1% Trypsin: 0.25% |
| Medium | DMEM |
Stem: Human | |
| |
| Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods.
, Cytotherapy
16,
1220-8, 2014 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Stem: Human | |
| |
| Douvaras, P., Wang, J., Zimmer, M., Hanchuk, S., O'Bara, M., Sadiq, S., Sim, F., Goldman, J. and Fossati, V.: Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells.
, Stem Cell Reports
3,
250, 2014 | |
| Cells: | Skin fibroblasts |
| Enzyme(s) | Collagenase Type 1: 1000 u/ml |
| Medium | DMEM |
Stem: Human | |
| |
| Avolio, E., Gianfranceschi, G., Cesselli, D., Caragnano, A., Athanasakis, E., Katare, R., Meloni, M., Palma, A., Barchiesi, A., Vascotto, C., Toffoletto, B., Mazzega, E., Finato, N., Aresu, G. and Livi, U.: Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction.
, Stem Cells 32,
2373, 2014 | |
| Cells: | Cardiac stem |
| Enzyme(s) | Collagenase Type 2: 0.25% |
| Medium | Joklik modified Eagle’s |
Stem: Human | |
| |
| Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells.
, Tiss Eng
19,
473-8, 2013 | |
| Cells: | Adipose stromal stem |
| Enzyme(s) | Collagenase animal free: 200 u/ml |
| Medium | DMEM/Hams F-12 |
Stem: Human | |
| |
| Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia.
, PLoS ONE
8,
e56749, 2013 | |
| Cells: | Dental pulp stem cells |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Stem: Human | |
| |
| Patel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta.
, Stem Cells Transl Med 2,
839-47, 2013 | |
| Cells: | Endothelial colony forming |
| Enzyme(s) | Collagenase Type 1: 0.1% Deoxyribonuclease I: 0.1% Neutral Protease: 0.075% |
| Medium | HBSS |
Stem: Human | |
| |
| Nagel, S., Rohr, F., Weber, C., Kier, J., Siemers, F., Kruse, C., Danner, S., Brandenburger, M. and Matthiessen, A.: Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro.
, PLoS ONE
8,
e78365, 2013 | |
| Cells: | Epidermal stem cells |
| Enzyme(s) | Neutral Protease: 2 u/ml Trypsin: 2.5% |
| Medium | DMEM |
Stem: Human | |
| |
| Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium.
, Front Physiol
4,
357, 2013 | |
| Cells: | Dental pulp stem |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| Welte, Y., Davies, C., Schafer, R. and Regenbrecht, C.: Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma.
, J Vis Exp
73,
e50200, 2013 | |
| Cells: | Melanoma |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | PBS |
Stem: Human | |
| |
| Barkauskas, C., Cronce, M., Rackley, C., Bowie, E., Keene, D., Stripp, B., Randell, S., Noble, P. and Hogan, B.: Type 2 Alveolar Cells are Stem Cells in Adult Lung
, J Clin Invest 123,
3025, 2013 | |
| Cells: | Alveolar epithelial |
| Enzyme(s) | Neutral Protease: 2 u/ml Trypsin: 0.25% Elastase: 10 u/ml |
| Medium | Bronchial Epithelial Growth Medium |
Stem: Human | |
| |
| Doi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis.
, J Tissue Eng Regen Med
7,
864, 2013 | |
| Cells: | Stromal vascular fraction |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | PBS |
Stem: Human | |
| |
| Han, Y., Tao, R., Sun, T., Chai, J., Xu, G. and Liu, J.: Optimization of Human Umbilical Cord Mesenchymal Stem Cell Isolation and Culture Methods.
, Cytotechnology
65,
819, 2013 | |
| Cells: | Umbilical cord mesenchymal stem |
| Enzyme(s) | Collagenase: 0.1-0.2% Trypsin: 0.1% |
| Medium | DMEM |
Stem: Human | |
| |
| Dobbin, Z. and Landen, C.: Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications.
, Curr Protoc Pharmacol
63,
Unit 14.28., 2013 | |
| Cells: | Cancer stem |
| Enzyme(s) | Trypsin: 0.25% Collagenase: 0.05% Hyaluronidase: 0.005% |
| Medium | RPMI |
Stem: Human | |
| |
| Sakai, K., Yamamoto, A., Matsubara, K., Nakamura, S., Naruse, M., Yamagata, M., Sakamoto, K., Tauchi, R., Wakao, N., Imagama, S., Hibi, H., Kadomatsu, K., Ishiguro, N. and Ueda, M: Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery After Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms.
, J Clin Invest 122,
80, 2012 | |
| Cells: | Dental pulp derived stem |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Stem: Human | |
| |
| Cervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance.
, Stem Cells Transl Med 1,
206-20, 2012 | |
| Cells: | Adipose-derived stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Stem: Human | |
| |
| Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1,
48, 2012 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.4% Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| James, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering.
, Stem Cells Transl Med 1,
673, 2012 | |
| Cells: | Perivascular stem |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Stem: Human | |
| |
| Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery.
, Stem Cells Transl Med 1,
230-6, 2012 | |
| Cells: | Adipose derived stromal vascular |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Stem: Human | |
| |
| Nazarov, I., Lee, J., Soupene, E., Etemad, S., Knapik, D., Green, W., Bashkirova, E., Fang, X., Matthay, M., Kuypers, F. and Serikov, V.: Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential.
, Stem Cells Transl Med 1,
359-72, 2012 | |
| Cells: | Chorionic mesenchymal |
| Enzyme(s) | Collagenase Type 1: 0.1% Neutral Protease: 2.5 u/ml Trypsin: 0.25% |
| Medium | MEM |
Stem: Human | |
| |
| Vatakis, D., Bristol, G., Kim, S., Levin, B., Liu, W., Radu, C., Kitchen, S. and Zack, J.: Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model.
, J Vis Exp
70,
e4181, 2012 | |
| Cells: | Liver hematopoietic |
| Enzyme(s) | Collagenase Type 4: 0.1% Hyaluronidase: 0.1% Deoxyribonuclease I: 2 u/ml |
| Medium | RPMI |
Stem: Human | |
| |
| Salehinejad, P., Alitheen, N., Ali, A., Omar, A., Mohit, M., Janzamin, E., Samani, F., Torshizi, Z. and Nematollahi-Mahani, S.: Comparison of Different Methods for the Isolation of Mesenchymal Stem Cells from Human Umbilical Cord Wharton's Jelly.
, In Vitro Cell Dev Biol Anim 48,
75, 2012 | |
| Cells: | Wharton jelly mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.1% Trypsin: 0.25% |
| Medium | DMEM |
Stem: Human | |
| |
| Vellasamy, S., Sandrasaigaran, P., Vidyadaran, S., George, E. and Ramasamy, R.: Isolation and Characterisation of Mesenchymal Stem cells Derived from Human Placenta Tissue.
, World J Stem Cells
4,
53-61, 2012 | |
| Cells: | Placental mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.4% Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Stem: Human | |
| |
| Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential
, Vasc Cell
3,
5, 2011 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase: 0.25% Deoxyribonuclease I: 0.002% |
| Medium | PBS |
Stem: Human | |
| |
| Farias, V., Linares-Fernandez, J., Penalver, J., Paya Colmenero, J., Ferron, G., Duran, E., Fernandez, R., Olivares, E., O'Valle, F., Puertas, A., Oliver, F. and Ruiz de Almodovar, J.: Human Umbilical Cord Stromal Stem Cell Express CD10 and Exert Contractile Properties.
, Placenta 32,
86, 2011 | |
| Cells: | Umbilical cord stromal stem |
| Enzyme(s) | Collagenase Type 4: 0.08% Neutral Protease: 0.138% Hyaluronidase: 0.02% |
| Medium | DMEM |
Stem: Human | |
| |
| Klein, D., Weisshardt, P., Kleff, V., Jastrow, H., Jakob, H., and Ergun, S.: Vascular Wall-Resident CD44+ Multipotent Stem Cells Vive Rise to Pericytes and Smooth Muscle Cells and Contribute to New Vessel Maturation.
, PLoS ONE
6,
e20540, 2011 | |
| Cells: | Vascular wall-resident multipotent stem cells |
| Enzyme(s) | Collagenase Type 2: 0.2% Elastase: 5 u/ml |
| Medium | see reference |
Stem: Human | |
| |
| Yang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells.
, J Biomed Sci
18,
59, 2011 | |
| Cells: | Adipose derived mesenchymal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | L-DMEM |
Stem: Human | |
| |
| Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates.
, Methods Mol Biol 702,
17-27, 2011 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/Ham's F-12 |
Stem: Human | |
| Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.: Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion.
, Neuro Oncol
13,
70-83, 2011 | |
| Cells: | Brain tumor |
| Enzyme(s) | Collagenase Type 1: 0.04% Hyaluronidase: 0.01% Deoxyribonuclease I: 0.02% Neutral Protease: 0.008% |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| Hjelmeland, A., Wu, Q., Wickman, S., Eyler, C., Heddleston, J., Shi, Q., Lathia, J., Macswords, J., Lee, J., McLendon, R. and Rich, J.: Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth.
, PLoS Biol
8,
e1000319, 2010 | |
| Cells: | Glioma stem cells |
| Enzyme(s) | PDS kit: per instructions |
| Medium | neurobasal medium |
Stem: Human | |
| |
| Roig, A., Eskiocak, U., Hight, S., Kim, S., Delgado, O., Souza, R., Spechler, S., Wright, W. and Shay, J.: Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro.
, Gastroenterol 138,
1012, 2010 | |
| Cells: | Colonic epithelial |
| Enzyme(s) | Collagenase: 150 u/ml Neutral Protease: 0.04 mg/ml |
| Medium | Basal X media |
Stem: Human | |
| |
| Du, Y., Roh, D., Funderburgh, M., Mann, M., Marra, K., Rubin, J., Li, X. and Funderburgh, J.: Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro.
, Mol Vis
16,
2680, 2010 | |
| Cells: | Corneal stromal stem |
| Enzyme(s) | Neutral Protease: 1.2 u/ml Collagenase: 0.1% |
| Medium | DMEM |
Stem: Human | |
| |
| Huang, G., Yamaza, T., Shea, L., Djouad, F., Kuhn, N., Tuan, R. and Shi, S.: Stem/Progenitor Cell-Mediated De Novo Regeneration of Dental Pulp with Newly Deposited Continuous Layer of Dentin in an In Vivo Model.
, Tissue Eng Part A
16,
605, 2010 | |
| Cells: | Dental pulp and apical papilla stem cells |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | MEM |
Stem: Human | |
| |
| Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering.
, J Biomed Mater Res 92,
979, 2010 | |
| Cells: | Adipose tissue-derived stem |
| Enzyme(s) | Collagenase Type 2: 1.0% |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| Zeddou, M., Briquet, A., Relic, B., Josse, C., Malaise, M., Gothot, A., Lechanteur, C. and Beguin, Y.: The Umbilical Cord Matrix is a Better Source of Mesenchymal Stem Cells (MSC) Than the Umbilical Cord Blood.
, Cell Biol Int 34,
693-701, 2010 | |
| Cells: | Umbilical cord mesenchymal |
| Enzyme(s) | Hyaluronidase: 0.05% Collagenase: 0.08% |
| Medium | DMEM |
Stem: Human | |
| |
| Hareendran, S., Sathishkumar, S., Abbas, S., Mackay, A. and Rajan, P.: A Novel Composition for the Culture of Human Adipose Stem Cells which Includes Complement C3.
, Cytotechnology
62,
389-402, 2010 | |
| Cells: | Adipose stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Stem: Human | |
| |
| Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes.
, Cytotherapy
12,
538, 2010 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/Hams F-12 |
Stem: Human | |
| |
| Huang, E., Hynes, M., Zhang, T., Ginestier, C., Dontu, G., Appelman, H., Fields, J., Wicha, M. and Boman, B.: Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis.
, Cancer Res 69,
3382-9, 2009 | |
| Cells: | Colonic epithelial |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | not listed |
Stem: Human | |
| |
| Sun, N., Panetta, N., Gupta, D., Wilson, K., Lee, A., Jia, F., Hu, S., Cherry, A., Robbins, R., Longaker, M. and Wu, J.: Feeder-Free Derivation of Induced Pluripotent Stem Cells from Adult Human Adipose Stem Cells.
, Proc Natl Acad Sci U S A 106,
15720, 2009 | |
| Cells: | Adult human adipose stem cells |
| Enzyme(s) | Collagenase Type 2: 0.075% |
| Medium | DMEM |
Stem: Human | |
| |
| Varghese, M., Olstorn, H., Berg-Johnsen, J., Moe, M., Murrell, W. and Langmoen, I.: Isolation of Human Multipotent Neural Progenitors from Adult Filum Terminale.
, Stem Cells Dev
18,
603, 2009 | |
| Cells: | Filum terminale neural progenitor |
| Enzyme(s) | Trypsin: see reference |
| Medium | DMEM/F-12 |
Stem: Human | |
| |
| Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.: Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.
,Neuro Oncol Vol. 11,, ,
109, 2009 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 4: 0.1% Hyaluronidase: 0.07% Deoxyribonuclease I: 0.04% |
| Medium | see reference |
Stem: Human | |
| |
| Cai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function.
, Stem Cells 27,
230, 2009 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Stem: Human | |
| |
| Zhang, Q., Yamaza, T., Kelly, AP, Shi, S., Wang, S., Brown, J., Wang, L., French, S., Shi, S. and Le, A.: Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis.
, PLoS ONE
4,
e7798, 2009 | |
| Cells: | Skin and keloid progenitor |
| Enzyme(s) | Collagenase Type 1: 0.4% Neutral Protease: 0.3% |
| Medium | PBS |
Stem: Human | |
| |
| Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion.
, EMBO Mol Med
1,
338-51, 2009 | |
| Cells: | Colon cancer |
| Enzyme(s) | Collagenase Type 1: 300 u/ml Hyaluronidase: 100 u/ml |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| Kossack, N., Meneses, J., Shefi, S., Nguyen, H., Chavez, S., Nicholas, C., Gromoll, J., Turek, P. and Reijo-Pera, R.: Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells.
, Stem Cells ,
, 2008 | |
| Cells: | Spermatogonial stem cells |
| Enzyme(s) | Collagenase: 1% Deoxyribonuclease I: 0.22% Trypsin: 0.4% |
| Medium | DMEM |
Stem: Human | |
| |
| Nesti, L., Jackson, W., Shanti, R., Koehler, S., Aragon, A., Bailey, J., Sracic, M., Freedman, B., Giuliani, J. and Tuan, R.: Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue.
, J Bone Joint Surg Am
90,
2390, 2008 | |
| Cells: | Muscle derived multiprogenitor cells |
| Enzyme(s) | Collagenase Type 2: 0.05% |
| Medium | DMEM |
Stem: Human | |
| |
| Jeong, J.: Adipose Stem Cells as a Clinically Available and Effective Source of Adult Stem Cell Therapy, Int J Stem Cells 1,
43, 2008 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | DMEM |
Stem: Human | |
| |
| Wauthier, E., Schmelzer, E., Turner, W., Zhang, L., LeCluyse, E., Ruiz, J., Turner, R., Furth, M., Kubota, H., Lozoya, O., Barbier, C., McClelland, R., Yao, H., Moss, N., Bruce, A., Ludlow, J. and Reid, L.: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance.
, Methods Cell Biol 86,
137, 2008 | |
| Cells: | Hepatic stem cells and heptoblasts |
| Enzyme(s) | Collagenase Type 4: 0.014-0.06% |
| Medium | various |
Stem: Human | |
| |
| Secco, M., Zucconi, E., Vieira, N., Fogaca, L.,Cerqueira, A., Carvalho, M., Jazedje, T., Okamoto, O., Muotri, A. and Zatz, M.: Multipotent Stem Cells from Umbilical Cord: Cord is Richer Than Blood!
, Stem Cells 26,
146, 2008 | |
| Cells: | Umbilical mesenchymal stem |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | DMEM |
Stem: Human | |
| |
| Pilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells.
, Regen Med
3,
705-15, 2008 | |
| Cells: | Stem |
| Enzyme(s) | Collagenase: 280 u/ml |
| Medium | D-PBS |
Stem: Human | |
| |
| Welter, J., Solchaga, L.and Penick, K.: Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay
, Biotechniques
42,
732, 2007 | |
| Cells: | Bone marrow derived MSC |
| Enzyme(s) | Trypsin: 0.05% Papain: 0.0025% |
| Medium | DMEM |
Stem: Human | |
| |
| Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche
, Nat Med 13,
1219-27, 2007 | |
| Cells: | Tendon stem/progenitor |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Stem: Human | |
| |
| Li, C., Heidt, D., Dalerba, P., Burant, C., Zhang, L., Adsay, V., Wicha, M., Clarke, M. and Simeone, D.: Identification of Pancreatic Cancer Stem Cells.
, Cancer Res 67,
1030, 2007 | |
| Cells: | Pancreatic cancer stem cells |
| Enzyme(s) | Collagenase Type 4: 200 u/ml |
| Medium | medium 199 |
Stem: Human | |
| |
| Lei, L., Liao, W., Sheng, P., Fu, M., He, A. and Huang, G.: Biological Character of Human Adipose-Derived Adult Stem Cells and Influence of Donor Age on Cell Replication in Culture.
, Sci China C Life Sci
50,
320, 2007 | |
| Cells: | Adipose derived adult stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Stem: Human | |
| |
| Steigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta.
, Curr Protoc Stem Cell Biol
Chapter 1,
Unit 1E.2, 2007 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 10% Neutral Protease: see reference |
| Medium | CMF-DPBS |
Stem: Human | |
| |
| Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors.
, J Cell Physiol 212,
702-9, 2007 | |
| Cells: | Adipose derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F12 |
Stem: Human | |
| |
| Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling.
, Stem Cells 25,
628-38, 2007 | |
| Cells: | Oral epithelial |
| Enzyme(s) | Neutral Protease: 1.2 u/ml Trypsin: 0.05% |
| Medium | PBS |
Stem: Human | |
| |
| Portmann-Lanz CBettina, Schoeberlein Andreina, Huber Alexander, Sager Ruth, Malek Antoine, Holzgreve Wolfgang, Surbek DanielV: Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration
, Am J Obstet Gynecol
194,
664-73, 2006 | |
| Cells: | Placental mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 270 u/ml Neutral Protease: 2.4 u/ml |
| Medium | MEM |
Stem: Human | |
| |
| Mitchell James B, McIntosh Kevin, Zvonic Sanjin, Garrett Sara, Floyd Z Elizabeth, Kloster Amy, Di Halvorsen Yuan, Storms Robert W, Goh Brian, Kilroy Gail, Wu Xiying, Gimble Jeffrey M: Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers
, Stem Cells 24,
376-85, 2006 | |
| Cells: | Adipose-derived adult stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F-12 Ham's |
Stem: Human | |
| |
| Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK
, J Lipid Res 47,
653-64, 2006 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | HBSS |
Stem: Human | |
| |
| Kern Susanne, Eichler Hermann, Stoeve Johannes, Kluter Harald, Bieback Karen: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue
, Stem Cells 24,
1294-301, 2006 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | DMEM |
Stem: Human | |
| |
| Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei: Isolation of a novel population of multipotent adult stem cells from human hair follicles
, Am J Pathol 168,
1879-88, 2006 | |
| Cells: | Hair follicular epithelial |
| Enzyme(s) | Neutral Protease: 1.25% Trypsin: 0.25% |
| Medium | DMEM/F-12 |
Stem: Human | |
| |
| Boquest Andrew C, Shahdadfar Aboulghassem, Brinchmann Jan E, Collas Philippe: Isolation of stromal stem cells from human adipose tissue
, Methods Mol Biol 325,
35-46, 2006 | |
| Cells: | Stromal stem cells |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Stem: Human | |
| |
| Guilak, F., Lott, K., Awad, H., Cao, Q., Hicok, K., Fermor, B. and Gimble, J.: Clonal Analysis of the Differentiation Potential of Human Adipose-Derived Adult Stem Cells.
, J Cell Physiol 206,
229, 2006 | |
| Cells: | Adipose derived adult stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM/F-12 |
Stem: Human | |
| |
| Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture
, Mol Biol Cell
16,
1131-41, 2005 | |
| Cells: | Adipocytes, stromal vascular |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | HBSS |
Stem: Human | |
| |
| Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress.
, J Biomech Eng 127,
1081, 2005 | |
| Cells: | Adult stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Stem: Human | |
| |
| Li, A., Pouliot, N., Redvers, R., and Kaur, P.: Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny
, J Clin Invest 113,
390-400, 2004 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Neutral Protease: 0.4% Collagenase: 0.3% |
| Medium | DMEM |
Stem: Human | |
| |
| Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells
, Circulation 110,
349-55, 2004 | |
| Cells: | Stromal vascular, adipocytes, stem |
| Enzyme(s) | Collagenase: 300 u/ml |
| Medium | PBS |
Stem: Human | |
| |
| Aust, L., Devlin, B., Foster, S., Halvorsen, Y., Hicok, K., du Laney, T., Sen, A., Willingmyre, G. and Gimble, J.: Yield of Human Adipose-Derived Adult Stem Cells from Liposuction Aspirates.
, Cytometry 6,
7-14, 2004 | |
| Cells: | Adipose derived adult stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM-Ham's F-12 |
Stem: Human | |
| |
| Miura, M., Gronthos, S, Zhao, M, Lu, B, Fisher, L, Robey, P, and Shi, S.: SHED: Stem Cells from Human Exfoliated Deciduous Teeth
, Proc Natl Acad Sci U S A 100,
5807, 2003 | |
| Cells: | Stem cells Human exfoliated deciduous teeth |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | see reference |
Stem: Human | |
| |
| Papini S, Cecchetti D, Campani D, Fitzgerald W, Grivel J, Chen S, Margolis L, Revoltella R.: Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture
, Stem Cells 21,
481, 2003 | |
| Cells: | Human epidermal keratinocyte stem cells |
| Enzyme(s) | Neutral Protease: 0.5% |
| Medium | DMEM |
Stem: Human | |
| |
| Covas DT, Siufi JL C, Silva AR L, Orellana MD: Isolation and culture of umbilical vein mesenchymal stem cells
, Braz J Med Biol Res
36,
1179-83, 2003 | |
| Cells: | Umbilical vein mesenchymal stem cells |
| Enzyme(s) | Collagenase: 1% |
| Medium | PBS |
Stem: Human | |
| |
| Zuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells
, Mol Biol Cell
13,
4279-95, 2002 | |
| Cells: | Processed lipoaspirate cells |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | PBS |
Stem: Human | |
| |
| Malhi, H., Irani, A., Gagandeep, S. and Gupta, S.: Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes.
, J Cell Sci
115,
2679, 2002 | |
| Cells: | Epithelial progenitor |
| Enzyme(s) | Collagenase: 0.03% |
| Medium | DMEM |
Stem: Human | |
| |
| Zhang, S., Wernig, M., Duncan, I., Brustle, O., and Thomson, J.: In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells
, Nat Biotechnol
19,
1129, 2001 | |
| Cells: | Stem, embryonic |
| Enzyme(s) | Neutral Protease: 0.01% - 0.02% |
| Medium | DMEM |
Stem: Human | |
| |
| Kambe, N, Kambe, M, Kochan, J, and Schwartz ,L.: Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes
, Blood 97,
2045, 2001 | |
| Cells: | Human skin mast cells |
| Enzyme(s) | Collagenase Type 2: 0.15% Hyaluronidase: 0.07% Deoxyribonuclease I: 0.03% |
| Medium | HBSS |
Stem: Human | |
| |
| Uchida, N., Buck, D., He, D., Reitsma, M., Masek, M., Phan, T., Tsukamoto, A., Gage, F. and Weissman, I.: Direct Isolation of Human Central Nervous System Stem Cells.
, Proc Natl Acad Sci U S A 97,
14720, 2000 | |
| Cells: | Central nervous system stem |
| Enzyme(s) | Collagenase: 0.1% Hyaluronidase: 0.1% |
| Medium | HBSS |
Stem: Human | |
| |
| Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts
, Science 282,
1145-7, 1998 | |
| Cells: | Embryonic stem |
| Enzyme(s) | Neutral Protease: 1% Collagenase Type 4: 0.1% |
| Medium | DMEM |
Stem: Human | |
| |
| Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio: Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages
, Lancet 364,
1872-83, | |
| Cells: | Muscle-derived stem cells |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | DMEM/F12 |
Stem: Insect | |
| |
| Berger, C., Harzer, H., Burkard, T., Steinmann, J., van der Horst, S., Laurenson, A., Novatchkova, M., Reichert, H. and Knoblich, J.: FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal.
, Cell Rep
2,
407, 2012 | |
| Cells: | Neuroblasts |
| Enzyme(s) | Collagenase Type 1: 0.1% Papain: 0.1% |
| Medium | Rinaldini solution |
Stem: Monkey | |
| |
| Chen, S., Revoltella, R., Papini, S., Michelini, M., Fitzgerald, W., Zimmerberg, J.,and Margolis, L.: Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems
, Stem Cells 21(3),
281, 2003 | |
| Cells: | Embryonic stem |
| Enzyme(s) | Collagenase Type 4: 0.08% |
| Medium | DMEM |
Stem: Monkey | |
| |
| Nagano, M., McCarrey, J., and Brinster, R.: Primate Spermatogonial Stem Cells Colonize Mouse Testes
, Biol Reprod 64,
1409, 2001 | |
| Cells: | Primate spermatogonial |
| Enzyme(s) | Collagenase Type 2: 0.1% Trypsin: 0.05% Deoxyribonuclease I: 0.1% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Pham, K., Delitto, D., Knowlton, A., Hartlage, E., Madhavan, R., Gonzalo, D., Thomas, R., Behrns, K., George, T., Hughes, S., Wallet, S., Liu, C., Trevino, J.: Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture.
, Am J Pathol 186,
, 2016 | |
| Cells: | Pancreatic cancer |
| Enzyme(s) | Stemxyme 1: 0.2% |
| Medium | HBSS |
Stem: Mouse | |
| |
| Birbrair, A, Sattiraju, A, Zhu, D, Zulato, G, Batista, I, Nguyen, V, Messi M, Solingapuram Sai, K, Marini, F, Delbono, O and Mintz, A.: Novel Peripherally Derived Neural-Like Stem Cells as Therapeutic Carriers for Treating Glioblastomas.
, Stem Cells Transl Med ,
, 2016 | |
| Cells: | Muscle derived NLSC |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Maria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis.
, Cytotherapy
18,
1129-45, 2016 | |
| Cells: | Adipose mesenchyal stromal |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Wang, H., Chen, H., Feng, B., Wang, X., He, X., Hu, R., Yin, M., Wang, W., Fu, W. and Xu, Z.: Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue.
, BMC Biotechnol
14,
75, 2014 | |
| Cells: | Cardiac progenitor |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor.
, J Vis Exp
,
, 2014 | |
| Cells: | Epithelial stem |
| Enzyme(s) | Collagenase: 2% |
| Medium | DMEM/F12 |
Stem: Mouse | |
| |
| Martin, L. and Seandel, M.: Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines.
, J Vis Exp
72,
e50017, 2013 | |
| Cells: | Spermatogonial stem |
| Enzyme(s) | Trypsin: 0.05% Collagenase Type 1: 0.03% Deoxyribonuclease I: 80 u/ml |
| Medium | DMEM |
Stem: Mouse | |
| |
| Hotta, R., Stamp, L., Foong, J., McConnell, S., Bergner, A., Anderson, R., Enomoto, H., Newgreen, D., Obermayr, F., Furness, J. and Young, H.: Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon.
, J Clin Invest 123,
1182, 2013 | |
| Cells: | Enteric neural crest progenitors |
| Enzyme(s) | Neutral Protease: 0.5% Collagenase animal free: 0.05% |
| Medium | DMEM/F12 |
Stem: Mouse | |
| |
| Mazzoleni, S., Jachetti, E., Morosini, S., Grioni, M., Piras, I., Pala, M., Bulfone, A., Freschi, M., Bellone, M. and Galli, R.: Gene Signatures Distinguish Stage-Specific Prostate Cancer Stem Cells Isolated From Transgenic Adenocarcinoma of the Mouse Prostate Lesions and Predict the Malignancy of Human Tumors.
, Stem Cells Transl Med 2,
678, 2013 | |
| Cells: | Prostate tumor |
| Enzyme(s) | Collagenase Type 4: 1,600 u/ml |
| Medium | DMEM/F12 |
Stem: Mouse | |
| |
| Liu, X., Johnson, S., Liu, S., Kanojia, D., Yue, W., Singh, U., Wang, Q, Wang Qi, Nie, Q. and Chen H.: Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy.
, Sci Rep
3,
2473, 2013 | |
| Cells: | Mammary tumor |
| Enzyme(s) | Collagenase: 0.15% Hyaluronidase: 0.020% |
| Medium | DMEM/F12 |
Stem: Mouse | |
| |
| Huch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis.
, EMBO J
32,
2708-21, 2013 | |
| Cells: | Pancreas organoid |
| Enzyme(s) | Collagenase: 0.012% Neutral Protease: 0.012% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Masaki, T., Qu, J., Cholewa-Waclaw, J., Burr, K., Raaum, R. and Rambukkana, A.: Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection.
, Cell
152,
51, 2013 | |
| Cells: | Schwann |
| Enzyme(s) | Collagenase Type 1: 0.01% Trypsin: 0.125% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Xue, X. and Shah, Y.: In Vitro Organoid Culture of Primary Mouse Colon Tumors.
, J Vis Exp
,
e50210, 2013 | |
| Cells: | Colon tumor organoids |
| Enzyme(s) | Collagenase Type 4: 200 u/ml Neutral Protease: 0.01% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Jensen, U., Ghazizadeh, S. and Owens, D.: Isolation and Characterization of Cutaneous Epithelial Stem Cells.
, Methods Mol Biol 989,
61-9, 2013 | |
| Cells: | Epithelial stem |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 0.4% |
| Medium | HBSS |
Stem: Mouse | |
| |
| Barthel, E., Speer, A., Levin, D., Sala, F., Hou, X., Torashima, Y., Wigfall, C. and Grikscheit, T.: Tissue Engineering of the Intestine in a Murine Model.
, J Vis Exp
70,
e4279, 2012 | |
| Cells: | Intestinal organoids |
| Enzyme(s) | Collagenase Type 1: 800 u/ml Neutral Protease: 0.013% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Ziegler, A., Schneider, J., Qin, M., Tyler, W., Pintar, J., Fraidenraich, D., Wood, T and Levison, S.: IGF-II Promotes Stemness of Neural Restricted Precursors.
, Stem Cells 30,
1265, 2012 | |
| Cells: | Neuroshere |
| Enzyme(s) | Trypsin: 0.25% Papain: 100 u Deoxyribonuclease I: 0.025% |
| Medium | Pro-N |
Stem: Mouse | |
| |
| Takahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair.
, Stem Cells Transl Med 1,
803, 2012 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium.
, PLoS ONE
7,
e45517, 2012 | |
| Cells: | Synovial mesenchymal |
| Enzyme(s) | Collagenase: 0.1% Deoxyribonuclease I: 0.005% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Vaughan, A., Halbert, C., Wootton, S. and Miller, A.: Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation.
, Mol Cancer Res
10,
86, 2012 | |
| Cells: | Lung tumor |
| Enzyme(s) | Neutral Protease: 50 u/ml Collagenase: 400 u/ml Deoxyribonuclease I: 50 u/ml |
| Medium | DMEM |
Stem: Mouse | |
| |
| Bracko, O., Singer, T., Aigner, S., Knobloch, M., Winner, B., Ray, J., Clemenson, G., Suh, H., Couillard-Despres, S., Aigner, L., Gage, F. and Jessberger, S.: Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis.
, J Neurosci 32,
3376-87, 2012 | |
| Cells: | Neural stem cells |
| Enzyme(s) | Papain: 0.01% Neutral Protease: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F12 |
Stem: Mouse | |
| |
| Salisbury, E., Lazard, Z., Ubogu, E., Davis, A. and Olmsted-Davis, E.: Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2.
, Stem Cells Transl Med 1,
874-85, 2012 | |
| Cells: | Nerve progenitors |
| Enzyme(s) | Collagenase Type 4: 0.025% Trypsin NF 1:250: .025% |
| Medium | HBSS |
Stem: Mouse | |
| |
| Sugii, S., Kida, Y., Berggren, W. and Evans, R.: Feeder-Dependent and Feeder-Independent iPS Cell Derivation from Human and Mouse Adipose Stem Cells.
, Nat Protoc
6,
346, 2011 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 1: 0.025% |
| Medium | HBSS |
Stem: Mouse | |
| |
| Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands.
, Immunity
34,
590, 2011 | |
| Cells: | Spleen, bone marrow endothelial |
| Enzyme(s) | Collagenase Type 4: 0.3-1.0% Deoxyribonuclease I: 20 u/ml |
| Medium | PBS |
Stem: Mouse | |
| |
| Chow, K., Jun, D., Helm, K., Wagner, D. and Majka, S.: Isolation & Characterization of Hoechst(low) CD45(negative) Mouse Lung Mesenchymal Stem Cells.
, J Vis Exp
56,
e3159, 2011 | |
| Cells: | Lung mesenchymal stem |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | HBSS |
Stem: Mouse | |
| |
| Janebodin, K., Horst, O., Ieronimakis, N., Balasundaram, G., Reesukumal, K., Pratumvinit, B. and Reyes, M.: Isolation and Characterization of Neural Crest-Derived Stem Cells from Dental Pulp of Neonatal Mice.
, PLoS ONE
6,
e27526, 2011 | |
| Cells: | Dental pulp stem |
| Enzyme(s) | Collagenase Type 4: 0.2% Neutral Protease: 1.2 u/ml |
| Medium | PBS |
Stem: Mouse | |
| |
| Rountree, C., Ding, W., Dang, H., Vankirk, C. and Crooks, G.: Isolation of CD133+ Liver Stem Cells for Clonal Expansion.
, J Vis Exp
56,
e3183, 2011 | |
| Cells: | CD133+ stem |
| Enzyme(s) | Collagenase Type 4: 0.05% Pronase: 0.05% Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F12 |
Stem: Mouse | |
| |
| Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells.
, Blood 115,
957, 2010 | |
| Cells: | Stem and progenitor |
| Enzyme(s) | Collagenase Type 2: 0.2% |
| Medium | HBSS |
Stem: Mouse | |
| |
| Kobayashi, T., Yamaguchi, T., Hamanaka, S., Kato-Itoh, M., Yamazaki, Y., Ibata, M., Sato, H., Lee, Y., Usui, J., Knisely, A., Hirabayashi, M. and Nakauchi, H.: Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells.
, Cell
142,
787, 2010 | |
| Cells: | Islets |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | RPMI 1640 |
Stem: Mouse | |
| |
| Xu, S., De Becker, A., Van Camp, B., Vanderkerken, K. and Van Riet, I.: An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells.
, J Biomed Biotechnol
2010,
105940, 2010 | |
| Cells: | Bone marrow mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.25% |
| Medium | RPMI 1640 |
Stem: Mouse | |
| |
| Nakamura, Y., Arai, F., Iwasaki, H., Hosokawa, K., Kobayashi, I., Gomei, Y., Matsumoto, Y., Yoshihara, H. and Suda, T.: Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells.
, Blood 116,
1422, 2010 | |
| Cells: | Endosteal cells |
| Enzyme(s) | Collagenase Type 1: 0.3% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Rasheed, Z., Wang, Q. and Matsui, W.: Isolation of Stem Cells from Human Pancreatic Cancer Xenografts.
, J Vis Exp
43,
2169, 2010 | |
| Cells: | Pancreatic cancer stem |
| Enzyme(s) | Collagenase Type 4: 200 u/ml Neutral Protease: 0.6 u/ml |
| Medium | DMEM |
Stem: Mouse | |
| |
| Burger, P., Gupta, R., Xiong, X., Ontiveros, C., Salm, S., Moscatelli, D. and Wilson, E.: High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells.
, Stem Cells 27,
2220-8, 2009 | |
| Cells: | Prostate epithelial/stem |
| Enzyme(s) | Collagenase Type 2: 0.5% Trypsin: 0.05% |
| Medium | HBSS |
Stem: Mouse | |
| |
| Deleyrolle, L. and Reynolds, B.: Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells using the Neurosphere Assay.
, Methods Mol Biol 549,
91, 2009 | |
| Cells: | Adult neural stem |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Cho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model.
, Stem Cells 27,
259-65, 2009 | |
| Cells: | Adipose tissue-derived stem |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | Modified Eagles |
Stem: Mouse | |
| |
| Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H. and Matsuzaki, Y.: Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow.
, J Exp Med 206,
2483-96, 2009 | |
| Cells: | Bone marrow |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Taddei, I., Deugnier, M., Faraldo, M., Petit, V., Bouvard, D., Medina, D., Fassler, R., Thiery, J. and Glukhova, M.: Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells
, Nat Cell Biol 10,
716-22, 2008 | |
| Cells: | Mammary epithelial |
| Enzyme(s) | Collagenase: 0.3% Hyaluronidase: 100 u/ml Trypsin: 0.25% Neutral Protease: 0.5% Deoxyribonuclease I: 0.01% |
| Medium | see reference |
Stem: Mouse | |
| |
| Sher, F., Rossler, R., Brouwer, N., Balasubramaniyan, V., Boddeke, E. and Copray, S.: Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2.
, Stem Cells 26,
2875, 2008 | |
| Cells: | Astrocytes |
| Enzyme(s) | Trypsin: 0.25% Deoxyribonuclease I: 1,000 u/ml |
| Medium | HBSS |
Stem: Mouse | |
| |
| Breault, D., Min, I., Carlone, D., Farilla, L., Ambruzs, D., Henderson, D., Algra, S., Montgomery, R., Wagers, A. and Hole, N.: Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells.
, Proc Natl Acad Sci U S A 105,
10420, 2008 | |
| Cells: | Germ cells |
| Enzyme(s) | Collagenase Type 1: 100 u/ml |
| Medium | HBSS |
Stem: Mouse | |
| |
| Hutton, S. and Pevny, L.: Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System., Cold Spring Harb. Protoc. 11,
5077, 2008 | |
| Cells: | Neural progenitor cell |
| Enzyme(s) | Papain: 10 ul/ml |
| Medium | PBS |
Stem: Mouse | |
| |
| Staszkiewicz, J., Gimble, J., Manuel, J. and Gawronska-Kozak, B.: IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential.
, Stem Cells 26,
2666, 2008 | |
| Cells: | Ear mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | DMEM/F12 |
Stem: Mouse | |
| |
| Gekas, C., Rhodes, K. and Mikkola, H.: Isolation and Analysis of Hematopoietic Stem Cells from the Placenta.
, J Vis Exp
16,
e742, 2008 | |
| Cells: | Hematopoietic stem cells |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | PBS |
Stem: Mouse | |
| |
| Lu Shi-Jiang, Feng Qiang, Caballero Sergio, Chen Yu, Moore Malcolm A, Grant Maria B, Lanza Robert: Generation of functional hemangioblasts from human embryonic stem cells
, Nat Methods
4,
501-9, 2007 | |
| Cells: | HES-BC cells |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche
, Nat Med 13,
1219-27, 2007 | |
| Cells: | Tendon stem/progenitor |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Prince, M., Sivanandan, R., Kaczorowski, A., Wolf, G., Kaplan, M., Dalerba, P., Weissman, I., Clarke, M. and Ailles, L.: Identification of a Subpopulation of Cells with Cancer Stem Cell Properties in Head and Neck Squamous Cell Carcinoma.
, Proc Natl Acad Sci U S A 104,
973, 2007 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 3: 200 u/ml |
| Medium | RPMI-1640 |
Stem: Mouse | |
| |
| Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse
, Stem Cells 24,
322-32, 2006 | |
| Cells: | Liver epithelial progenitor cells |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.05% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Di Rocco Giuliana, Iachininoto Maria Grazia, Tritarelli Alessandra, Straino Stefania, Zacheo Antonella, Germani Antonia, Crea Filippo, Capogrossi Maurizio C: Myogenic potential of adipose-tissue-derived cells
, J Cell Sci
119,
2945-52, 2006 | |
| Cells: | Adipose mesenchymal stem |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | PBS |
Stem: Mouse | |
| |
| Meletis, K., Wirta, V., Hede, S., Nister, M., Lundeberg, J. abd Frisen, J.: p53 Suppresses the Self-Renewal of Adult Neural Stem Cells.
, Development
133,
363, 2006 | |
| Cells: | Neural stem cells |
| Enzyme(s) | Papain: see reference |
| Medium | DMEM/F-12 |
Stem: Mouse | |
| |
| Dekel, B., Zangi, L., Shezen, E., Reich-Zeliger, S., Eventov-Friedman, S., Katchman, H., Jacob-Hirsch, J., Amariglio, N., Rechavi, G., Margalit, R. and Reisner, Y.: Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney.
, J Am Soc Nephrol
17,
3300, 2006 | |
| Cells: | Kidney |
| Enzyme(s) | Collagenase Type 4: 0.075% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.: Isolation of Neural Stem Cells from the Postnatal Cerebellum
, Nat Neurosci 8,
723, 2005 | |
| Cells: | Cerebellar stem cells |
| Enzyme(s) | Papain: 10u/ml Deoxyribonuclease I: 250 u/ml |
| Medium | Dulbecco's PBS |
Stem: Mouse | |
| |
| Klein C, Butt SJ, Machold RP, Johnson JE, and Fishell G.: Cerebellum- and forebrain-derived stem cells possess intrinsic regional character
, Development
132,
4497, 2005 | |
| Cells: | Neurosperes |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM/F12 |
Stem: Mouse | |
| |
| Schatten Gerald, Smith Joseph, Navara Christopher, Park Jong-Hyuk, Pedersen Roger: Culture of human embryonic stem cells
, Nat Methods
2,
455-63, 2005 | |
| Cells: | Embryonic fibroblast feeder cells |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Seaberg, R., Smukler, S. and Van der Kooy, D.: Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain.
, Dev Biol 278,
71, 2005 | |
| Cells: | Neural progenitor |
| Enzyme(s) | PDS kit: per instructions |
| Medium | see reference |
Stem: Mouse | |
| |
| Kotton, D., Fabian, A. and Mulligan, R.: A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity.
, Blood 106,
1574, 2005 | |
| Cells: | Liver derived stem cells |
| Enzyme(s) | Collagenase Type 1: 0.1% Neutral Protease: 2.4 u/ml |
| Medium | HBSS |
Stem: Mouse | |
| |
| Bertoncello, I. and Williams, B.: Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining.
, Methods Mol Biol 263,
181, 2004 | |
| Cells: | Bone marrow |
| Enzyme(s) | Collagenase Type 1: 0.15% Neutral Protease: 0.15% |
| Medium | PBS |
Stem: Mouse | |
| |
| Howell, J., Lee, W., Morrison, P., Zhong, J., Yoder, M. and Srour, E.: Pluripotent Stem Cells Identified in Multiple Murine Tissues.
, Ann N Y Acad Sci 996,
158, 2003 | |
| Cells: | Pluripotent stem cells |
| Enzyme(s) | Collagenase Type 1: 220 u/ml Neutral Protease: 33 u/ml |
| Medium | MEM |
Stem: Mouse | |
| |
| Gritti, A, Bonfanti, L, Doetsch, F, Caille, I, Alvarez-Buylla, A, Lim, D, Galli, R, Verdugo J, Herrera, D, and Vescovi A.: Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents
, J Neurosci 22(2),
437, 2002 | |
| Cells: | Neural stem cells |
| Enzyme(s) | Papain: 0.1% |
| Medium | DMEM/F-12 |
Stem: Mouse | |
| |
| McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.: Muscle-derived hematopoietic stem cells are hematopoietic in origin
, Proc Natl Acad Sci U S A 99,
1341, 2002 | |
| Cells: | Myocytes |
| Enzyme(s) | Collagenase Type 2: 0.2% Trypsin: 0.25% |
| Medium | HBSS |
Stem: Mouse | |
| |
| Estivill-Torrus, G., Pearson, H., Van Heyningen, V., Price, D. and Rashbass, P.: Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors.
, Development
129,
455, 2002 | |
| Cells: | Cortical progenitors |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Serum free medium |
Stem: Mouse | |
| |
| Boulanger CA, Smith GH: Reducing mammary cancer risk through premature stem cell senescence
, Oncogene
20,
2264-72, 2001 | |
| Cells: | Mammary epithelial stem |
| Enzyme(s) | Collagenase Type 3: 0.1% Hyaluronidase: 0.1% Pronase: 1.25% Deoxyribonuclease I: 0.2% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Dubey P, Wu H, Reiter RE, Witte ON: Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression
, Cancer Res 61,
3256-61, 2001 | |
| Cells: | Prostatic stem |
| Enzyme(s) | Collagenase Type 1: 170 u/ml |
| Medium | DMEM |
Stem: Mouse | |
| |
| Jackson, K., and Goodell, M.: Hematopoietic Potential of Stem Cells Isolated from Murine Skeletal Muscle
, Proc Natl Acad Sci U S A 96,
14482, 1999 | |
| Cells: | Muscle hematopoietic stem cells |
| Enzyme(s) | Collagenase: 0.2% Trypsin: 0.1% |
| Medium | DMEM |
Stem: Mouse | |
| |
| Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C, and Vescovi A.: Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain
, J Neurosci 19(9),
3287, 1999 | |
| Cells: | Neural subventricular zone |
| Enzyme(s) | Trypsin: 0.13% Hyaluronidase: 0.067% |
| Medium | DMEM/F12 |
Stem: Ovine | |
| |
| Letouzey, V., Tan, K., Deane, J., Ulrich, D., Gurung, S., Ong, Y. and Gargett, C.: Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium.
, PLoS ONE
10,
e0127531, 2015 | |
| Cells: | Endometrial stromal |
| Enzyme(s) | Collagenase Type 1: 0.5% Deoxyribonuclease I: 0.04 u/ml |
| Medium | DMEM/F-12 |
Stem: Porcine | |
| |
| Ando, W., Kutcher, J., Krawetz, R., Sen, A., Nakamura, N., Frank, C. and Hart, D.: Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage.
, Cytotherapy
16,
776, 2014 | |
| Cells: | Synovial membrane and skin stem |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | DMEM |
Stem: Porcine | |
| |
| Park, M., Park, J., Kim, M., Lee, K., Park, H., Yun, J., Choi, J., Lee, E. and Lee, S.: Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes.
, J Assist Reprod Genet
31,
983-91, 2014 | |
| Cells: | Testicular |
| Enzyme(s) | Collagenase Type 4: 0.1% Hyaluronidase: 0.1% Trypsin: 0.25% |
| Medium | DMEM |
Stem: Porcine | |
| |
| Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells.
, Cells Tissues Organs
188,
251, 2008 | |
| Cells: | Adipose mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Stem: Porcine | |
| |
| Dirami G, Ravindranath N, Pursel V, Dym M: Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM
, Biol Reprod 61,
225-30, 1999 | |
| Cells: | Seminiferous epithelial cells |
| Enzyme(s) | Collagenase: 0.15% Deoxyribonuclease I: .0001% Hyaluronidase: 0.15% Trypsin: 0.05% |
| Medium | DMEM/F12 |
Stem: Rabbit | |
| |
| Zhang, J. and Wang, J.: Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes.
, BMC Musculoskelet Disord
11,
10, 2010 | |
| Cells: | Tenocytes and tendon stem cells |
| Enzyme(s) | Collagenase Type 1: 0.3% Neutral Protease: 0.4% |
| Medium | DMEM |
Stem: Rat | |
| |
| Dayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells
, Cytotherapy
19,
937-946, 2017 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Stem: Rat | |
| |
| Jiang, H., Zhang, Y., Tian, K., Wang, B. and Han, S.: Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells.
, Sci Rep
7,
41837, 2017 | |
| Cells: | Placental mesenchymal |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.02% Neutral Protease: 0.1% |
| Medium | DMEM |
Stem: Rat | |
| |
| Ohta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury.
, Cytotherapy
19,
839-848, 2017 | |
| Cells: | Adipose stromal |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | DMEM |
Stem: Rat | |
| |
| Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model.
, Cytotherapy
17,
543-59, 2015 | |
| Cells: | Mesenchymal stem |
| Enzyme(s) | Collagenase Type 1: 0.075% |
| Medium | DMEM |
Stem: Rat | |
| |
| Veronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells.
, Cytotherapy
17,
215-23, 2015 | |
| Cells: | Mesenchymal stromal |
| Enzyme(s) | Collagenase Type 2: 0.075% |
| Medium | DMEM |
Stem: Rat | |
| |
| Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord.
, J Vis Exp
,
e52732, 2015 | |
| Cells: | Spinal cord neural progenitor |
| Enzyme(s) | PDS kit: with modifications |
| Medium | EBSS |
Stem: Rat | |
| |
| Duarte, S., Momier, D., Baque, P., Casanova, V., Loubat, A., Samson, M., Guigonis, J., Staccini, P., Saint-Paul, M., De Lima, M., Carle, G. and Pierrefite-Carle, V.: Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model.
, Stem Cells 31,
423-32, 2013 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 1: 0.16% Hyaluronidase: 0.002% Deoxyribonuclease I: 0.006% |
| Medium | DMEM/F12 |
Stem: Rat | |
| |
| Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose.
, J Biomed Biotechnol
2010,
104296, 2010 | |
| Cells: | Brown adipocytes |
| Enzyme(s) | Collagenase Type 4: 0.1% Neutral Protease: 0.1% Trypsin: 0.05% |
| Medium | PBS |
Stem: Rat | |
| |
| Wei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats.
, Stem Cells 27,
478, 2009 | |
| Cells: | Adipose derived stem |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | DMEM |
Stem: Rat | |
| |
| Mothe, A., Kulbatski, I., Van Bendegem, R., Lee, L., Kobayashi, E., Keating, A., and Tator, C.: Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells
, J Histochem Cytochem 53(10),
1215, 2005 | |
| Cells: | Spinal cord progenitor cells
Hybrid toxins |
| Enzyme(s) | PDS kit: see reference |
| Medium | Neurobasal A |
Stem: Rat | |
| |
| Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian: Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord
, Dev Neurosci 27,
20-6, 2005 | |
| Cells: | Neutral stem cells, Schwann cells |
| Enzyme(s) | Trypsin: 0.25% Collagenase: 0.16% |
| Medium | DMEM/F12 |
Stem: Rat | |
| |
| Gobbel GT, Choi SJ, Beier S, Niranjan A: Long-term cultivation of multipotential neural stem cells from adult rat subependyma
, Brain Res 980,
221, 2003 | |
| Cells: | Neural stem cells |
| Enzyme(s) | Papain: 0.09% Deoxyribonuclease I: 0.1% |
| Medium | EBSS |
Stem: Rat | |
| |
| Kruger, G., Mosher, J., Bixby, S., Joseph, N., Iwashita, T. and Morrison, S.: Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness.
, Neuron 35,
657, 2002 | |
| Cells: | Gut neural crest stem |
| Enzyme(s) | Collagenase Type 4: 0.1% Trypsin: 0.025% |
| Medium | HBSS |
Stem: Rat | |
| |
| Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity.
, Neuron 35,
643, 2002 | |
| Cells: | Sciatic nerve and gut neural crest stem |
| Enzyme(s) | Collagenase Type 4: 0.025% Trypsin: 0.005% Deoxyribonuclease I: 0.05% |
| Medium | HBSS |
Stem: Rat | |
| |
| Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells
, Cell
96,
737, 1999 | |
| Cells: | Sciatic nerves |
| Enzyme(s) | Trypsin: 0.025% Collagenase Type 3: 0.1% |
| Medium | L-15 medium (see reference) |
Stem: Rat | |
| |
| Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., and Frisen, J: Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System
, Cell
96,
25, 1999 | |
| Cells: | Spinal cord |
| Enzyme(s) | Trypsin: 0.133% |
| Medium | HBSS and PIPES |
Stem: Rat | |
| |
| Palmer, T., Markakis, E., Willhoite, A., Safar, F., and Gage, F.: Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS
, J Neurosci 19,
8487, 1999 | |
| Cells: | Neural stem cells |
| Enzyme(s) | Papain: 2.5 u/ml Deoxyribonuclease I: 250 u/ml Neutral Protease: 1 u/ml |
| Medium | DMEM/F-12 |
Stem: Rat | |
| |
| Ray, J. and Gage, F.: Neural Stem Cell Isolation, Characterization and Transplantation,Modern Techniques in Neuroscience Research,Johansson, H. and Windhorst, U., Springer-Verlag New York,
339, 1999 | |
| Cells: | Central nervous system stem cells |
| Enzyme(s) | Papain: 0.01% Neutral Protease: 0.1% |
| Medium | DMEM/F-12 |
Stem: Rat | |
| |
| Maric, D., Maric, I. and Barker, J.: Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells.
, Methods
16,
247, 1998 | |
| Cells: | Neurons and progenitor |
| Enzyme(s) | Papain: 20 u/ml |
| Medium | EBSS |
Stem: Rat | |
| |
| Stemple, D., and Anderson, D.: Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest
, Cell
71,
973, 1992 | |
| Cells: | Stem, neural crest |
| Enzyme(s) | Collagenase: 0.075% |
| Medium | Ringer's solution |
Thymus: Human | |
| |
| Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K: Human thymus contains 2 distinct dendritic cell populations
, Blood 97,
1733-41, 2001 | |
| Cells: | Dentritic |
| Enzyme(s) | Collagenase Type 2: 0.1% Deoxyribonuclease I: 0.002% |
| Medium | RPMI 1640 |
Thymus: Mouse | |
| |
| Rode, I. and Boehm, T.: Regenerative Capacity of Adult Cortical Thymic Epithelial Cells.
, Proc Natl Acad Sci U S A 109,
3463-8, 2012 | |
| Cells: | Thymic stromal |
| Enzyme(s) | Collagenase Type 4: 0.02% Neutral Protease: 0.02% Deoxyribonuclease I: 0.0025% |
| Medium | RPMI-1640 |
Thymus: Mouse | |
| |
| Phillips, Joy, Brondstetter, T., English, C., Lee, H., Virts, E, and Thoman, M.: IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution
, J Immunol ,
4869, 2004 | |
| Cells: | Stromal |
| Enzyme(s) | Collagenase Type 3: 0.2% Hyaluronidase: 0.1% |
| Medium | RPMI 1640 |
Thymus: Mouse | |
| |
| Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells
, J Exp Med 196,
1415, 2002 | |
| Cells: | Dentritic |
| Enzyme(s) | Collagenase Type 3: 0.1% Deoxyribonuclease I: 325 u/ml |
| Medium | RPMI 1640 |
Thymus: Mouse | |
| |
| Smith KM, Olson DC, Hirose R, Hanahan D: Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance
, Int Immunol 9,
1355-65, 1997 | |
| Cells: | Thymic |
| Enzyme(s) | Collagenase Type 3: 100-400 u/ml |
| Medium | HBSS |
Thymus: Mouse | |
| |
| Ropke, C., van Deurs, B., and Petersen, O.: Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium
, In Vitro Cell Dev Biol 26,
671, 1990 | |
| Cells: | Epithelial, thymus |
| Enzyme(s) | Neutral Protease: 1.5 µg/ml |
| Medium | DMEM |
Thymus: Mouse | |
| |
| Ehmann, U., Shiurba, R., and Peterson, W.: Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture
, In Vitro Cell Dev Biol 22 (12),
738, 1986 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | DMEM |
Thymus: Mouse | |
| |
| Jones, K. and Pierre, R.: Analysis of Cellular Heterogeneity in Mouse Thymus Cultures
, In Vitro 17,
431, 1981 | |
| Cells: | Thymus |
| Enzyme(s) | Collagenase Type 3: 150 u/ml |
| Medium | DMEM |
Thymus: Rat | |
| |
| Bonfanti, P., Claudinot, S., Amici, A., Farley, A., Blackburn, C. and Barrandon, Y.: Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells.
, Nature
466,
978, 2010 | |
| Cells: | Thymic |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | HBSS |
Thymus: Rat | |
| |
| Masuda, A and Matsuyama, M: Epithelial Cell Lines From Rat Thymoma and Rat Thymus,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
21C:4.1, 1995 | |
| Cells: | Thymic epithelial |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | DMEM |
Thymus: Rat | |
| |
| Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus
, In Vitro Cell Dev Biol 26,
713, 1990 | |
| Cells: | Epthelial |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | Eagle's MEM Serum-free |
Thyroid/Parathyroid: Bovine | |
| |
| Nygren, P., Gylfe, E., Larsson, R., Johansson, H., Juhlin, C., Klareskoq, L., Akerstrom, G., and Rastad, J.: Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism
, Biochim Biophys Acta 968,
253, 1988 | |
| Cells: | Parathyroid |
| Enzyme(s) | Deoxyribonuclease I: 0.005%, |
| Medium | HEPES Ham's F10 |
Thyroid/Parathyroid: Bovine | |
| |
| Wallace, J., and Scarpa, A.: Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations
, J Biol Chem 257,
10613, 1982 | |
| Cells: | Parathyroid glands |
| Enzyme(s) | Deoxyribonuclease I: 0.0075% |
| Medium | HEPES buffer |
Thyroid/Parathyroid: Bovine | |
| |
| Brown, E., Hurwitz, S., and Aurbach, G.: Preparation of Viable Isolated Bovine Parathyroid Cells
, Endocrinology 99,
1582, 1976 | |
| Cells: | Parathyroid |
| Enzyme(s) | Deoxyribonuclease I: 0.004% |
| Medium | Eagle's #2 medium without bicarbonate |
Thyroid/Parathyroid: Bovine | |
| |
| Tong, W.: The Isolation and Culture of Thyroid Cells
, Meth Enzymol 32,
745, 1974 | |
| Cells: | Thyroid |
| Enzyme(s) | Trypsin: 0.004% |
| Medium | EBSS |
Thyroid/Parathyroid: Chicken | |
| |
| Hilfer, S., and Brown, J.: Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart
, Exp Cell Res
65,
246, 1971 | |
| Cells: | Thyroid Muscle Heart |
| Enzyme(s) | Collagenase: 0.25% |
| Medium | Tyrode's saline, potassium free |
Thyroid/Parathyroid: Chicken | |
| |
| Spooner, B.: The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture
, J Cell Physiol 75,
33, 1970 | |
| Cells: | Thyroid follicular |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Tyrode's solution, CMF |
Thyroid/Parathyroid: Human | |
| |
| Patel, V., Logan, A., Watkinson, J., Uz-Zaman, S., Sheppard, M., Ramsden, J. and Eggo, M.: Isolation and Characterization of Human Thyroid Endothelial Cells.
, Am J Physiol Endocrinol Metab
284,
E168, 2003 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 2: 0.1% |
| Medium | DMEM |
Thyroid/Parathyroid: Human | |
| |
| Gianoukakis, A., Cao, H., Jennings, T. and Smith, T.: Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells.
, Am J Physiol Cell Physiol
280,
C701, 2001 | |
| Cells: | Thyrocytes |
| Enzyme(s) | Collagenase Type 1: 130 u/ml Neutral Protease: 0.5 u/ml |
| Medium | HBSS |
Thyroid/Parathyroid: Human | |
| |
| Howie, A., Walker, S., Akesson, B., Arthur, J. and Beckett, G.: Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis.
, Biochem J
308 ( Pt 3),
713, 1995 | |
| Cells: | Thyrocytes |
| Enzyme(s) | Neutral Protease: 0.5% Trypsin: 0.25% Collagenase: 0.1% |
| Medium | EBSS |
Thyroid/Parathyroid: Human | |
| |
| Miller, R., Hiraoka, T., Nakamura, N., Tenou, H., Kopecky, K., Jones, M. and Gould, M.: In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology.
, J Radiat Res
26,
269, 1985 | |
| Cells: | Thyroid |
| Enzyme(s) | Collagenase: 300 u/ml |
| Medium | Ham's F-12/MEM |
Thyroid/Parathyroid: Mouse | |
| |
| Kitajima, S., Kohno, S., Kondoh, A., Sasaki, N., Nishimoto, Y., Li, F., Mohammed, M., Muranaka, H., Nagatani, N., Suzuki, M., Kido, Y. and Takahashi, C.: Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts.
, Stem Cells 33,
1657-69, 2015 | |
| Cells: | Thyroid tumor |
| Enzyme(s) | Collagenase Type 1: 300 u/ml Hyaluronidase: 100 u/ml Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Thyroid/Parathyroid: Mouse | |
| |
| Martin, A., Coronel, E., Sano, G., Chen, S., Vassileva, G., Canasto-Chibuque, C., Sedgwick, J., Frenette, P., Lipp, M., Furtado, G. and Lira, S.: A Novel Model for Lymphocytic Infiltration of the Thyroid Gland Generated by Transgenic Expression of the CC Chemokine CCL21.
, J Immunol 173,
4791, 2004 | |
| Cells: | Thyroid |
| Enzyme(s) | Neutral Protease: 0.0012 u/ml Collagenase Type 2: 0.25 u/ml |
| Medium | RPMI 1640 |
Thyroid/Parathyroid: Ovine | |
| |
| Kerkof, P.: Preparation of Primary Cultures of Ovine Thyroid Gland Cells, J Tiss Cul Meth 7,
23, 1982 | |
| Cells: | Thyroid |
| Enzyme(s) | Collagenase: 0.2% |
| Medium | Puck's Saline F |
Thyroid/Parathyroid: Porcine | |
| |
| Tong, W.: The Isolation and Culture of Thyroid Cells
, Meth Enzymol 32,
745, 1974 | |
| Cells: | Thyroid |
| Enzyme(s) | Trypsin: 0.004% |
| Medium | EBSS |
Thyroid/Parathyroid: Rat | |
| |
| Arauchi, A., Shimizu, T., Yamato, M., Obara, T. and Okano, T.: Tissue-Engineered Thyroid Cell Sheet Rescued Hypothyroidism in Rat Models After Receiving Total Thyroidectomy Comparing with Nontransplantation Models.
, Tissue Eng Part A
15,
3943, 2009 | |
| Cells: | Thyroid |
| Enzyme(s) | Collagenase Type 2: 0.15% Collagenase Type 4: 0.15% |
| Medium | DMEM |
Tonsil: Human | |
| |
| Muto, M., Manfroi, B., Suzuki, H., Joh, K., Nagai, M., Wakai, S., Righini, C. et al: Toll-Like Receptor 9 Stimulation Induces Aberrant Expression of a Proliferation-Inducing Ligand by Tonsillar Germinal Center B Cells in IgA Nephropathy.
, J Am Soc Nephrol
28,
1227-1238, 2017 | |
| Cells: | Tonsillar |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | see reference |
Tonsil: Human | |
| |
| Schmidt, S., Schenkova, K., Adam, T., Erikson, E., Lehmann-Koch, J., Sertel, S., Verhasselt, B., Fackler, O., Lasitschka, F. and Keppler, O.: SAMHD1's Protein Expression Profile in Humans.
, J Leukoc Biol 98,
5-14, 2015 | |
| Cells: | Tonsillar |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | PBS |
Tonsil: Human | |
| |
| Sato, D., Suzuki, Y., Kano, T., Suzuki, H., Matsuoka, J., Yokoi, H., Horikoshi, S., Ikeda, K. and Tomino, Y.: Tonsillar TLR9 Expression and Efficacy of Tonsillectomy with Steroid Pulse Therapy in IgA Nephropathy Patients.
, Nephrol Dial Transplant
27,
1090-7, 2012 | |
| Cells: | Tonsillar |
| Enzyme(s) | Collagenase Type 4: 0.1% |
| Medium | Bambanker |
Tonsil: Human | |
| |
| Grammer Amrie C, Slota Rebecca, Fischer Randy, Gur Hanan, Girschick Hermann, Yarboro Cheryl, Illei Gabor G, Lipsky PeterE: Abnormal germinal center reactions in systemic lupus erythematosus demonstrated by blockade of CD154-CD40 interactions
, J Clin Invest 112,
1506-20, 2003 | |
| Cells: | Tonsillar mononuclear cells |
| Enzyme(s) | Collagenase Type 1: 210 u/ml Deoxyribonuclease I: 90 u/ml |
| Medium | RPMI |
Tonsil: Human | |
| |
| Grammer AC, McFarland RD, Heaney J, Darnell BF, Lipsky PE: Expression, regulation, and function of B cell-expressed CD154 in germinal centers
, J Immunol 163,
4150-9, 1999 | |
| Cells: | Tonsillar mononuclear cells |
| Enzyme(s) | Collagenase Type 1: 210 u/ml Deoxyribonuclease I: 90 u/ml |
| Medium | RPMI |
Tumor: Hamster | |
| |
| Gonzalez, A., Oberley, T., Schultz, J., Ostrom, J., and Li, J.: In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney
, In Vitro Cell Dev Biol 29A,
562, 1993 | |
| Cells: | Tumor |
| Enzyme(s) | Hyaluronidase: 0.1% |
| Medium | Waymouth's MB |
Tumor: Hamster | |
| |
| Min, B., Kim, K., Cherrick, H., and Park, N.: Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation
, In Vitro Cell Dev Biol 27A,
128, 1991 | |
| Cells: | Buccal pouch |
| Enzyme(s) | Neutral Protease: 0.24% |
| Medium | CMF HBSS |
Tumor: Human | |
| |
| Jeske, S., Schuler, P., Doescher, J., Theodoraki, M., Laban, S., Brunner, C., Hoffman, T. and Wigand, M.: Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma
, Immun Ageing
17,
3, 2020 | |
| Cells: | Tumor infiltrating lympocytes |
| Enzyme(s) | Collagenase Type 1: 200 u/ml |
| Medium | RPMI |
Tumor: Human | |
| |
| Phan, N., Hong, J., Tofig, B., Mapua, M., Elashoff, D., Moatamed, N., Huang, J., Memarzadeh, S., Damoiseaux, R. and Soragni, A.: A Simple High-Throughput Approach Identifies Actionable Drug Sensitivities in Patient-Derived Tumor Organoids.
, Commun Biol
2,
78, 2019 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 4: 200 u/ml |
| Medium | DMEM |
Tumor: Human | |
| |
| Ayuso, J., Vitek, R., Swick, A., Skala, M., Wisinski, K., Kimple, R., Lambert, P. and Beebe, D.: Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells
, Sci Rep
9,
12480, 2019 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Collagenase Type 1: 0.5% Neutral Protease: 0.1% Deoxyribonuclease I: 1,000 u/ml |
| Medium | FM Fibroblast media |
Tumor: Human | |
| Neal, J,, Li ,X., Zhu, J., Giangarra, V., Grzeskowiak, C., Ju, J., Liu, I. Chiou, S., Salahudeen, A. and Smith, A. et al: Organoid Modeling of the Tumor Immune Microenvironment.
, Cell
175,
1972, 2018 | |
| Cells: | Organoids |
| Enzyme(s) | Collagenase Type 4: 200-300 u/ml |
| Medium | DMEM |
Tumor: Human | |
| |
| Jiang, C., Zhang, Q., Shanti, R., Shi, S., Chang, T., Carrasco, L., Alawi, F. and Le, A.: Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells.
, Stem Cells 35,
2083-2094, 2017 | |
| Cells: | Ameloblastoma epithelial |
| Enzyme(s) | Collagenase: 0.1% Neutral Protease: 50 u/ml |
| Medium | MEM |
Tumor: Human | |
| |
| Pham, K., Delitto, D., Knowlton, A., Hartlage, E., Madhavan, R., Gonzalo, D., Thomas, R., Behrns, K., George, T., Hughes, S., Wallet, S., Liu, C., Trevino, J.: Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture.
, Am J Pathol 186,
, 2016 | |
| Cells: | Pancreatic cancer |
| Enzyme(s) | Stemxyme 1: 0.2% |
| Medium | HBSS |
Tumor: Human | |
| |
| Volovitz, I, Shapira, N., Ezer, H., Gafni, A., Lustgarten, M., Alter, T., Ben-Horin, I., Barzilai, O., Shahar, T., Kanner, A., Fried, I., Veshchev, I., Grossman, R. and Ram, Z,: A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells.
, BMC Neurosci
17,
30, 2016 | |
| Cells: | Brain tumor |
| Enzyme(s) | Neutral Protease: 0.11 u/ml Collagenase Type 4: 0.05% Hyaluronidase: 1,000 u/ml Deoxyribonuclease I: 5 u/ml |
| Medium | HBSS |
Tumor: Human | |
| |
| Liang, S., Marti, T., Dorn, P., Froment, L., Hall, S., Berezowska, S., Kocher, G., Schmid, R. and Peng, R.: Blocking the Epithelial-to-Mesenchymal Transition Pathway Abrogates Resistance to Anti-Folate Chemotherapy in Lung Cancer.
, Cell Death Dis
6,
e1824, 2015 | |
| Cells: | Non-small cell lung tumor |
| Enzyme(s) | Collagenase Type 1: 0.1% Collagenase Type 2: 0.25% |
| Medium | RPMI-1640 |
Tumor: Human | |
| |
| Quatromoni, J., Singhal, S., Bhojnagarwala, P., Hancock, W., Albelda, S. and Eruslanov, E.: An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells
, J Leukoc Biol 97,
201, 2015 | |
| Cells: | Lung tumor |
| Enzyme(s) | Collagenase Type 1: 45-60 u/ml Collagenase Type 2: 15-20 u/ml Collagenase Type 4: 45-60 u/ml Elastase: 0.002% Deoxyribonuclease I: 0.002% |
| Medium | DMEM/F12 |
Tumor: Human | |
| |
| Baldan, V., Griffiths, R., Hawkins, R. and Gilham, D: Efficient and Reproducible Generation of Tumour-Infiltrating Lymphocytes for Renal Cell Carcinoma
, Br J Cancer
112,
1510, 2015 | |
| Cells: | Tumour infiltrating lymphocytes |
| Enzyme(s) | Collagenase: 1% Hyaluronidase: 1% Deoxyribonuclease I: 3,000 u/ml |
| Medium | RPMI 1640 |
Tumor: Human | |
| |
| Ali, M., Anand, S., Tangella, K., Ramkumar, D. and Saif, T.: Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry.
, J Vis Exp
,
e52532, 2015 | |
| Cells: | Colon |
| Enzyme(s) | Trypsin: 0.1% Collagenase: 0.1% |
| Medium | HBSS |
Tumor: Human | |
| |
| Hasselbach, L., Irtenkauf, S., Lemke, N., Nelson, K., Berezovsky, A., Carlton, E., Transou, A., Mikkelsen, T. and deCarvalho, A.: Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry.
, J Vis Exp
,
e51088, 2014 | |
| Cells: | Glioblastoma tumor |
| Enzyme(s) | Trypsin: 0.025% Collagenase Type 4: 500 u/ml |
| Medium | HBSS |
Tumor: Human | |
| |
| Yan, K., Wu, Q., Yan, D., Lee, C., Rahim, N., Tritschler, I., DeVecchio, J., Kalady, M., Hjelmeland, A. and Rich, J.: Glioma Cancer Stem Cells Secrete Gremlin1 to Promote their Maintenance Within the Tumor Hierarchy.
, Genes Dev
28,
1085-100, 2014 | |
| Cells: | Glioma cancer stem |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal |
Tumor: Human | |
| |
| Welte, Y., Davies, C., Schafer, R. and Regenbrecht, C.: Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma.
, J Vis Exp
73,
e50200, 2013 | |
| Cells: | Melanoma |
| Enzyme(s) | Collagenase Type 4: 0.1% Deoxyribonuclease I: 0.01% |
| Medium | PBS |
Tumor: Human | |
| |
| Chou, J., Fitzgibbon, M., Mortales, C., Towlerton, A., Upton, M., Yeung, R., McIntosh, M. and Warren, E.: Phenotypic and Transcriptional Fidelity of Patient-Derived Colon Cancer Xenografts in Immune-Deficient Mice.
, PLoS ONE
8,
e79874, 2013 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 1: 0.1% Hyaluronidase: 0.01% Deoxyribonuclease I: 0.01% |
| Medium | DMEM/F12 |
Tumor: Human | |
| |
| Dobbin, Z. and Landen, C.: Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications.
, Curr Protoc Pharmacol
63,
Unit 14.28., 2013 | |
| Cells: | Cancer stem |
| Enzyme(s) | Trypsin: 0.25% Collagenase: 0.05% Hyaluronidase: 0.005% |
| Medium | RPMI |
Tumor: Human | |
| |
| Zhou, J, Belov, L., Solomon, M., Chan, C., Clarke, S. and Christopherson, R.: Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing.
, J Vis Exp
55,
e3322, 2011 | |
| Cells: | Colorectal cancer |
| Enzyme(s) | Collagenase Type 4: 2% Deoxyribonuclease I: 0.1% |
| Medium | RPMI 1640 |
Tumor: Human | |
| |
| Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.: Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion.
, Neuro Oncol
13,
70-83, 2011 | |
| Cells: | Brain tumor |
| Enzyme(s) | Collagenase Type 1: 0.04% Hyaluronidase: 0.01% Deoxyribonuclease I: 0.02% Neutral Protease: 0.008% |
| Medium | DMEM/F12 |
Tumor: Human | |
| |
| Quintana, E., Shackleton, M., Foster, H., Fullen, D., Sabel, M., Johnson, T. and Morrison, S.: Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized.
, Cancer Cell
18,
510, 2010 | |
| Cells: | Tumorigenic melanoma |
| Enzyme(s) | Collagenase Type 4: 200 u/ml Trypsin: 0.05% Deoxyribonuclease I: 50-100 u/ml |
| Medium | PBS |
Tumor: Human | |
| |
| Kim, M., Evans, D., Wang, H., Abbruzzese, J., Fleming, J. and Gallick, G.: Generation of Orthotopic and Heterotopic Human Pancreatic Cancer Xenografts in Immunodeficient Mice.
, Nat Protoc
4,
1670, 2009 | |
| Cells: | Pancreatic tumor |
| Enzyme(s) | Collagenase Type 4: 200 u/ml |
| Medium | RPMI-1640 |
Tumor: Human | |
| |
| Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.: Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.
,Neuro Oncol Vol. 11,, ,
109, 2009 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 4: 0.1% Hyaluronidase: 0.07% Deoxyribonuclease I: 0.04% |
| Medium | see reference |
Tumor: Human | |
| |
| Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion.
, EMBO Mol Med
1,
338-51, 2009 | |
| Cells: | Colon cancer |
| Enzyme(s) | Collagenase Type 1: 300 u/ml Hyaluronidase: 100 u/ml |
| Medium | DMEM/F12 |
Tumor: Human | |
| |
| Liu, R., Wang, X., Chen, G., Dalerba, P., Gurney, A., Hoey, T., Sherlock, G., Lewicki, J., Shedden, K. and Clarke, M.: The Prognostic Role of a Gene Signature from Tumorigenic Breast-Cancer Cells.
, N Engl J Med 356,
217, 2007 | |
| Cells: | Breast epithelial |
| Enzyme(s) | Collagenase Type 3: 200 u/ml |
| Medium | HBSS |
Tumor: Human | |
| |
| Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi: Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R
, Am J Pathol 165,
533-40, 2004 | |
| Cells: | Prostate stromal cells |
| Enzyme(s) | Collagenase Type 1: 0.1% |
| Medium | RPMI 1640 |
Tumor: Human | |
| |
| Nishio Jun, Iwasaki Hiroshi, Ishiguro Masko, Ohjimi Yuko, Fujita Chikako, Isayama Teruto, Naito Masatoshi, Oda Yoshinao, Kaneko Yasuhiko, Kikuchi Masahiro: Establishment of a new human synovial sarcoma cell line, FU-SY-1, that expresses c-Met receptor and its ligand hepatocyte growth factor
, Int J Oncol
21,
17-23, 2002 | |
| Cells: | Human synovial sarcoma |
| Enzyme(s) | Collagenase Type 2: 200 u/ml |
| Medium | DMEM/F-12 |
Tumor: Human | |
| |
| Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes
, J Nutr 46,
96-105, 2001 | |
| Cells: | Colonocytes |
| Enzyme(s) | Collagenase: 0.15% |
| Medium | DMEM/F12 |
Tumor: Human | |
| |
| MacLeod, R: Rapid Monolayer Primary Cell Culture from Tissue Biopsy,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons Ltd,
3E:2.1, 1995 | |
| Cells: | Epithelial, fibroblasts |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | Ham's F-12 |
Tumor: Human | |
| |
| Hague, A and Paraskeva, C: Colon Adenocarcinoma Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd.,
12C:1.1, 1995 | |
| Cells: | Colon adenocarcinoma |
| Enzyme(s) | Hyaluronidase: 100 u/ml |
| Medium | DMEM |
Tumor: Human | |
| |
| Beaupain, R., Mainquene, C., Brouty-Boye, D., Planchon, P., and Magniew, V.: "Normal" Breast Cells Adjacent to a Tumor Grown in Long-term Three Dimensional Culture
, In Vitro Cell Dev Biol 29,
100, 1993 | |
| Cells: | Tumor, breast |
| Enzyme(s) | Hyaluronidase: 100 u/ml |
| Medium | DMEM |
Tumor: Human | |
| |
| Kruse, C., Mitchell, D., Kleinschmidt-DeMasteis, B., Franklin, W., Morse, H., Spector, E., and Lillehei, K.: Characterization of a Continuous Human Glioma Cell Line DBTRG-OSMG: Growth Kinetics, Karyotype, Receptor Expression and Tumor Suppressor Gene Analyses
, In Vitro Cell Dev Biol 28,
609, 1992 | |
| Cells: | Glioma |
| Enzyme(s) | Hyaluronidase: 0.01% |
| Medium | HBSS |
Tumor: Human | |
| |
| Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples
, In Vitro Cell Dev Biol 26,
1186, 1990 | |
| Cells: | Epithelial |
| Enzyme(s) | Collagenase: 2.0% |
| Medium | DMEM/Ham's F-12 |
Tumor: Human | |
| |
| Boyd, J., Rinehart Jr., C., Walton, L., Siegal, G. and Kaufman, D.: Ultrastructural Characterization of Two New Human Endometrial Carcinoma Cell Lines and Normal Human Endometrial Epithelial Cells Cultured on Extracellular Matrix
, In Vitro Cell Dev Biol 26,
701, 1990 | |
| Cells: | Tumor |
| Enzyme(s) | Neutral Protease: 0.24% |
| Medium | DMEM/Ham's F-12 |
Tumor: Human | |
| |
| Sheela S, Riccardi VM, Ratner N: Angiogenic and invasive properties of neurofibroma Schwann cells
, J Cell Biol
111,
645-53, 1990 | |
| Cells: | Neurofibroma |
| Enzyme(s) | Neutral Protease: 1.25 u/ml Collagenase Type 1: 0.05% Hyaluronidase: 0.1% |
| Medium | L-15 |
Tumor: Human | |
| |
| Sacks, P., Parnes, S., Gallick, G., Mansouri, Z., Lichtner, R., Satya-Prakash, K., Pathak, S, and Parsons, D.: Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck
, Cancer Res 48,
2858, 1988 | |
| Cells: | Tumor |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | DMEM |
Tumor: Human | |
| |
| Brattain, M., Marks, M., McCombs, J., Finely, W., and Brattain, D.: Characterization of Human Colon Carcinoma Cell Lines Isolated From a Single Primary Tumour
, Br J Cancer
47,
373, 1983 | |
| Cells: | Tumor, colon |
| Enzyme(s) | Trypsin: 0.25% |
| Medium | McCoy's |
Tumor: Human | |
| |
| Friedman, E., Higgins, P., Lipkin, M., Shinya, H., and Gelb, A.: Tissue Culture of Human Epithelial Cells from Benign Colonic Tumors
, In Vitro 17,
632, 1981 | |
| Cells: | Epthelial and tumor Colon |
| Enzyme(s) | Collagenase: 300 u/ml |
| Medium | PBS medium 199 or medium F 12 |
Tumor: Human | |
| |
| Leung, C., and Shiu, R.: Morphological and Proliferative Characteristics of Human Breast Tumor Cells Cultured on Plastic and in Collagen Matrix
, In Vitro 18,
476, 1981 | |
| Cells: | Tumor, breast |
| Enzyme(s) | Neuraminidase: 0.8 u/ml |
| Medium | HBSS |
Tumor: Human | |
| |
| Creasey, A., Smith, H., Hackett, A., Fukuyama, K., Epstein, W., and Madin, S.: Biological Properties of Human Melanoma Cells in Culture
, In Vitro 15,
342, 1979 | |
| Cells: | Melanoma Metastatic tumors |
| Enzyme(s) | Collagenase Type 3: 0.10% |
| Medium | DMEM |
Tumor: Human | |
| |
| Lasfargues, E.: Human Mammary Tumors,Tissue Culture Methods/Applications,Kruse, P., and Patterson, M., Academic Press,
45, 1973 | |
| Cells: | Mammary tumors, hard |
| Enzyme(s) | Collagenase: 0.10% |
| Medium | RPMI-1640 w/ 5% Fetal Calf Serum |
Tumor: Mouse | |
| |
| Walker, S., Wankell, M., Ho, V., White, R., Deo, N., Devine, C., Dewdney, B., Bhathal, P., Govaere, O., Roskams, T., Qiao, L., George, J. and Hebbard, L.: Targeting mTOR and Src Restricts Hepatocellular Carcinoma Growth in a Novel Murine Liver Cancer Model.
, PLoS ONE
14,
e0212860, 2019 | |
| Cells: | liver tumor |
| Enzyme(s) | Collagenase Type 2: 0.25% |
| Medium | HBSS |
Tumor: Mouse | |
| |
| Neal, J,, Li ,X., Zhu, J., Giangarra, V., Grzeskowiak, C., Ju, J., Liu, I. Chiou, S., Salahudeen, A. and Smith, A. et al: Organoid Modeling of the Tumor Immune Microenvironment.
, Cell
175,
1972, 2018 | |
| Cells: | Organoids |
| Enzyme(s) | Collagenase Type 4: 200-300 u/ml |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Erdreich-Epstein, A., Singh, A., Joshi, S., Vega, F., Guo, P., Xu, J., Groshen, S., Ye, W., Millard, M., Campan, M., Morales, G., Garlich, J., Laird, P., Seeger, R., Shimada, H. and Durden, D.: Association of High Microvessel A,B and Low PTEN with Poor Outcome in Stage 3 Neuroblastoma: Rationale for Using First in Class Dual PI3K/BRD4 Inhibitor, SF1126.
, Oncotarget
8,
52193-52210, 2017 | |
| Cells: | Tumor derived neutroblastoma |
| Enzyme(s) | Papain: 10 u/ml Deoxyribonuclease I: 250 u/ml |
| Medium | DPBS |
Tumor: Mouse | |
| |
| Boyd, N., Walker, K., Fried, J., Hackney, J., McDonald, P., Benavides, G., Spina, R., Audia, A., Scott, S., Libby, C., Tran, A., Bevensee, M., Griguer, C., Nozell, S., Gillespie, G. and Nabors, B.: Addition of Carbonic Anhydrase 9 Inhibitor SLC-0111 to Temozolomide Treatment Delays Glioblastoma Growth In Vivo.
, JCI Insight
2,
, 2017 | |
| Cells: | Glioblastoma |
| Enzyme(s) | PDS kit: per instructions |
| Medium | DMEM/F12 |
Tumor: Mouse | |
| |
| Filtjens, J., Keirsse, J., Van Ammel, E., Taveirne, S., Van Acker, A., Kerre, T., Taghon, T., Vandekerckhove, B., Plum, J., Van Ginderachter, J. and Leclercq, G.: Expression of the Inhibitory Ly49E Receptor is Not Critically Involved in the Immune Response Against Cutaneous, Pulmonary or Liver tumours.
, Sci Rep
6,
30564, 2016 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 4: 0.1% Neutral Protease: 0.1% Deoxyribonuclease I: 0.02% |
| Medium | RPMI-1640 |
Tumor: Mouse | |
| |
| Aguilera, T., Rafat, M., Castellini, L., Shehade, H., Kariolis, M., Hui, A., Stehr, H., von Eyben, R., Jiang, D., Ellies, L., Koong, A., Diehn, M., Rankin, E. Graves, E. and Giaccia, A.: Reprogramming the Immunological Microenvironment through Radiation and Targeting Axl.
, Nat Commun
7,
13898, 2016 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase: 0.1% Neutral Protease: 0.5 u/ml |
| Medium | F12K |
Tumor: Mouse | |
| |
| Singer, M., Wang, C., Cong, L., Marjanovic, N., Kowalczyk, Mo., Zhang, H., Nyman, J., Sakuishi, K., Kurtulus, S., Gennert, D., Xia, J., Kwon, J., Nevin, J., Herbst, R., Yanai, I., Rozenblatt-Rosen, O. and Kuchroo, V.: A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells.
, Cell
166,
1500-1511.e9, 2016 | |
| Cells: | Tumor-infiltrating lymphocytes |
| Enzyme(s) | Collagenase Type 4: 0.25% |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Otvos, B., Silver, D., Mulkearns-Hubert, E., Alvarado, A., Turaga, S., Sorensen, M., Rayman, P., Flavahan, W., Hale, J., Stoltz, K., Sinyuk, M., Wu, Q., Jarrar, A., Kim, S., Fox, P., Nakano, I. and Rich, J.: Cancer Stem Cell-Secreted Macrophage Migration Inhibitory Factor Stimulates Myeloid Derived Suppressor Cell Function and Facilitates Glioblastoma Immune Evasion.
, Stem Cells 34,
2026-39, 2016 | |
| Cells: | Glioblastoma |
| Enzyme(s) | PDS kit: per instructions |
| Medium | Neurobasal |
Tumor: Mouse | |
| |
| Blanco, S., Bandiera, R., Popis, M., Hussain, S., Lombard, P., Aleksic, J., Sajini, A., Tanna, H., Cortes-Garrido, R., Gkatza, N., Dietmann, S. and Frye, M.: Stem Cell Function and Stress Response are Controlled by Protein Synthesis.
, Nature
534,
335-40, 2016 | |
| Cells: | Keratinocytes |
| Enzyme(s) | Trypsin: 0.25% Collagenase Type 1: 0.12% Collagenase Type 2: 0.05% Collagenase Type 4: 0.05% Hyaluronidase: 0.1% |
| Medium | Low calcium containing media |
Tumor: Mouse | |
| |
| Hosseini, H., Obradovic, M., Hoffmann, M., Harper, K., Sosa, M., Werner-Klein, M., Nanduri, L., Werno, C., Ehrl, C., Maneck, M., Patwary, N., Haunschild, G., Guzvic, M., Reimelt, C. et al.: Early Dissemination Seeds Metastasis in Breast Cancer.
, Nature
,
, 2016 | |
| Cells: | Mammary tumor |
| Enzyme(s) | Collagenase Type 1: 200 u/ml Hyaluronidase: 0.001% |
| Medium | DMEM/F12 |
Tumor: Mouse | |
| |
| Xiao, L., McCann, J. and Dudley, A.: Isolation and Culture Expansion of Tumor-specific Endothelial Cells.
, J Vis Exp
,
e53072, 2015 | |
| Cells: | Endothelial |
| Enzyme(s) | Collagenase Type 2: 0.1% Neutral Protease: 0.25 u/ml Deoxyribonuclease I: 0.0075% |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Kitajima, S., Kohno, S., Kondoh, A., Sasaki, N., Nishimoto, Y., Li, F., Mohammed, M., Muranaka, H., Nagatani, N., Suzuki, M., Kido, Y. and Takahashi, C.: Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts.
, Stem Cells 33,
1657-69, 2015 | |
| Cells: | Thyroid tumor |
| Enzyme(s) | Collagenase Type 1: 300 u/ml Hyaluronidase: 100 u/ml Deoxyribonuclease I: 0.01% |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Devaud, C., Westwood, J., John, L., Flynn, J., Paquet-Fifield, S., Duong, C., Yong, C., Pegram, H., Stacker, S., Achen, M. Stewart, T., Snyder, L., Teng, M., Smyth, M., Darcy, P. and Kershaw, M: Tissues in Different Anatomical Sites can Sculpt and Vary the Tumor Microenvironment to Affect Responses to Therapy.
, Mol Ther
22,
18-27, 2014 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 4: 0.1 Hyaluronidase: 0.01% Deoxyribonuclease I: 30 u/ml |
| Medium | PBS |
Tumor: Mouse | |
| |
| Ali, O., Verbeke, C., Johnson, C., Sands, R., Lewin, S., White, D., Doherty, E., Dranoff, G. and Mooney, D.: Identification of Immune Factors Regulating Antitumor Immunity Using Polymeric Vaccines with Multiple Adjuvants.
, Cancer Res 74,
1670-81, 2014 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 1: 250 u/ml |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Dunleavey, J., Xiao, L., Thompson, J., Kim, M., Shields, J., Shelton, S., Irvin, D., Brings, V., Ollila, D., Brekken, R., Dayton, P., Melero-Martin, J. and Dudley, A.: Vascular Channels Formed by Subpopulations of PECAM1+ Melanoma Cells.
, Nat Commun
5,
5200, 2014 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 2: 0.2% Neutral Protease: 2.5 u/ml Deoxyribonuclease I: 0.0075% |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Sharon, Y., Alon, L., Glanz, S., Servais, C. and Erez, N.: Isolation of Normal and Cancer-Associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS).
, J Vis Exp
71,
e4425, 2013 | |
| Cells: | Fibroblasts |
| Enzyme(s) | Collagenase Type 2: 0.25% Collagenase Type 4: 0.25% Deoxyribonuclease I: 0.05% |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Mazzoleni, S., Jachetti, E., Morosini, S., Grioni, M., Piras, I., Pala, M., Bulfone, A., Freschi, M., Bellone, M. and Galli, R.: Gene Signatures Distinguish Stage-Specific Prostate Cancer Stem Cells Isolated From Transgenic Adenocarcinoma of the Mouse Prostate Lesions and Predict the Malignancy of Human Tumors.
, Stem Cells Transl Med 2,
678, 2013 | |
| Cells: | Prostate tumor |
| Enzyme(s) | Collagenase Type 4: 1,600 u/ml |
| Medium | DMEM/F12 |
Tumor: Mouse | |
| |
| Liu, X., Johnson, S., Liu, S., Kanojia, D., Yue, W., Singh, U., Wang, Q, Wang Qi, Nie, Q. and Chen H.: Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy.
, Sci Rep
3,
2473, 2013 | |
| Cells: | Mammary tumor |
| Enzyme(s) | Collagenase: 0.15% Hyaluronidase: 0.020% |
| Medium | DMEM/F12 |
Tumor: Mouse | |
| |
| Chen, X., Stewart, E., Shelat, A., Qu, C., Bahrami, A., Hatley, M., Wu, G., Bradley, C., McEvoy, J., Pappo, A., Spunt, S., Valentine, M., Valentine, V., Krafcik, F., Lang, W., Wierdl, M. and Tsurkan, L.: Targeting Oxidative Stress in Embryonal Rhabdomyosarcoma.
, Cancer Cell
24,
710-24, 2013 | |
| Cells: | Rhabdomycosarcoma tumor |
| Enzyme(s) | Trypsin: 0.012% Collagenase Type 2: 0.1% |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Kazerounian, S., Gerald, D., Huang, M., Chin, R, Udayakumar, D, Zheng, N., O'Donnell, R., Perruzzi, C., Mangiante, L., Pourat, J., Phung, T., Bravo-Nuevo, A., Shechter, S., McNamara, S. and Duhadaway, K.: RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis.
, Cancer Res 73,
50, 2013 | |
| Cells: | Tumor endothelial |
| Enzyme(s) | Collagenase Type 1: 0.2% |
| Medium | HBSS |
Tumor: Mouse | |
| |
| Watkins, S., Zhu, Z., Watkins, K. and Hurwitz, A.: Isolation of Immune Cells from Primary Tumors.
, J Vis Exp
64,
e3952, 2012 | |
| Cells: | Dendritic, macrophages |
| Enzyme(s) | Collagenase (1 or 4): 100-200 u/ml Deoxyribonuclease I: 0.01% |
| Medium | RPMI |
Tumor: Mouse | |
| |
| Vaughan, A., Halbert, C., Wootton, S. and Miller, A.: Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation.
, Mol Cancer Res
10,
86, 2012 | |
| Cells: | Lung tumor |
| Enzyme(s) | Neutral Protease: 50 u/ml Collagenase: 400 u/ml Deoxyribonuclease I: 50 u/ml |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Hamzah, J., Kotamraju, V., Seo, J., Agemy, L., Fogal, V., Mahakian, L., Peters, D., Roth, L., Gagnon, M., Ferrara, K. and Ruoslahti, E.: Specific Penetration and Accumulation of a Homing Peptide Within Atherosclerotic Plaques of Apolipoprotein E-deficient Mice.
, Proc Natl Acad Sci U S A 108,
7154, 2011 | |
| Cells: | Macrophages |
| Enzyme(s) | Collagenase Type 4: 0.2% |
| Medium | PBS |
Tumor: Mouse | |
| |
| Rasheed, Z., Wang, Q. and Matsui, W.: Isolation of Stem Cells from Human Pancreatic Cancer Xenografts.
, J Vis Exp
43,
2169, 2010 | |
| Cells: | Pancreatic cancer stem |
| Enzyme(s) | Collagenase Type 4: 200 u/ml Neutral Protease: 0.6 u/ml |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Kwong, B., Roberts, S., Silberzahn, T., Filler, R., Neustadter, J., Galan, A., Reddy, S., Lin, W., Ellis, P., Langford, C., Hayday, A. and Girardi, M.: Molecular Analysis of Tumor-Promoting CD8+ T cells in Two-Stage Cutaneous Chemical Carcinogenesis.
, J Invest Dermatol 130,
1726, 2010 | |
| Cells: | Tumor infiltration lymphocytes |
| Enzyme(s) | Collagenase Type 1: 0.25% Collagenase Type 2: 0.15% Collagenase Type 4: 0.1% Hyaluronidase: 0.025% |
| Medium | RPMI 1640 |
Tumor: Mouse | |
| |
| Kim, M., Evans, D., Wang, H., Abbruzzese, J., Fleming, J. and Gallick, G.: Generation of Orthotopic and Heterotopic Human Pancreatic Cancer Xenografts in Immunodeficient Mice.
, Nat Protoc
4,
1670, 2009 | |
| Cells: | Pancreatic tumor |
| Enzyme(s) | Collagenase Type 4: 200 u/ml |
| Medium | RPMI-1640 |
Tumor: Mouse | |
| |
| Prince, M., Sivanandan, R., Kaczorowski, A., Wolf, G., Kaplan, M., Dalerba, P., Weissman, I., Clarke, M. and Ailles, L.: Identification of a Subpopulation of Cells with Cancer Stem Cell Properties in Head and Neck Squamous Cell Carcinoma.
, Proc Natl Acad Sci U S A 104,
973, 2007 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 3: 200 u/ml |
| Medium | RPMI-1640 |
Tumor: Mouse | |
| |
| Berger, M., Bergers, G., Arnold, B., Hammerling, G. and Ganss, R.: Regulator of G-protein Signaling-5 Induction in Pericytes Coincides With Active Vessel Remodeling During Neovascularization
, Blood 105,
1094, 2005 | |
| Cells: | Tumor endothelial |
| Enzyme(s) | Collagenase Type 4: 500 u/ml Collagenase Type 2: 550 u/ml Deoxyribonuclease I: 3 u/ml |
| Medium | PBS |
Tumor: Mouse | |
| |
| Oliver, T., Read, T., Kessler, J., Mehmeti, A., Wells, J., Huynh, T., Lin, S. and Wechsler-Reya, R.: Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma.
, Development
132,
2425, 2005 | |
| Cells: | Granule cell precursors, pre-neoplastic and tumor cells |
| Enzyme(s) | Papain: 10 u/ml Deoxyribonuclease I: 250 u/ml |
| Medium | Neurobasal/B27 |
Tumor: Mouse | |
| |
| Hida Kyoko, Hida Yasuhiro, Amin Dhara N, Flint Alan F, Panigrahy Dipak, Morton Cynthia C, Klagsbrun Michael: Tumor-associated endothelial cells with cytogenetic abnormalities
, Cancer Res 64,
8249-55, 2004 | |
| Cells: | Tumor associated endothelial cells |
| Enzyme(s) | Collagenase Type 2: |
| Medium | see reference |
Tumor: Mouse | |
| |
| Bergers Gabriele, Song Steven, Meyer-Morse Nicole, Bergsland Emily, Hanahan Douglas: Benefits of targeting both pericytes and endothelial cells in the tumor vasculature with kinase inhibitors
, J Clin Invest 111,
1287-95, 2003 | |
| Cells: | Pancreatic tumor |
| Enzyme(s) | Collagenase Type 2: 0.5% Collagenase Type 4: 0.5% Deoxyribonuclease I: 0.2% |
| Medium | PBS |
Tumor: Mouse | |
| |
| Uekusa Yasuhiro, Yu Wen-Gong, Mukai Takao, Gao Ping, Yamaguchi Nobuya, Murai Masako, Matsushima Kouji, Obika Satoshi, Imanishi Takeshi, Higashibata Yuji, Nomura Shintaro, Kitamura Yukihiko, Fujiwara Hiromi, Hamaoka Toshiyuki: A pivotal role for CC chemokine receptor 5 in T-cell migration to tumor sites induced by interleukin 12 treatment in tumor-bearing mice
, Cancer Res 62,
3751-8, 2002 | |
| Cells: | Tumor-infiltrating lymphocyte |
| Enzyme(s) | Collagenase: 200 u/ml |
| Medium | RPMI 1640 |
Tumor: Mouse | |
| |
| Arbiser JL, Raab G, Rohan RM, Paul S, Hirschi K, Flynn E, Price ER, Fisher DE, Cohen C, Klagsbrun M: Isolation of mouse stromal cells associated with a human tumor using differential diphtheria toxin sensitivity
, Am J Pathol 155,
723-9, 1999 | |
| Cells: | Melanoma tumor cells |
| Enzyme(s) | Collagenase Type 2: 0.5% |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels
, In Vitro 16,
502, 1980 | |
| Cells: | Mammary tumors Epithelial |
| Enzyme(s) | Collagenase: 1.0% |
| Medium | HBSS |
Tumor: Mouse | |
| |
| Hosick, H.: A Note on Growth Patterns of Epithelial Tumor Cells in Primary Culture
, Cancer Res 34,
259, 1974 | |
| Cells: | Neoplastic Epithelial tumor |
| Enzyme(s) | Trypsin: |
| Medium | DMEM |
Tumor: Mouse | |
| |
| Kopelovich, L., Abraham, S., McGrath, H., DeOme, K., Chaikoff, I.: Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland
, Cancer Res 26,
1534, 1966 | |
| Cells: | Mammary |
| Enzyme(s) | Trypsin NF 1:250: 0.25% |
| Medium | HBSS |
Tumor: Rat | |
| |
| Duarte, S., Momier, D., Baque, P., Casanova, V., Loubat, A., Samson, M., Guigonis, J., Staccini, P., Saint-Paul, M., De Lima, M., Carle, G. and Pierrefite-Carle, V.: Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model.
, Stem Cells 31,
423-32, 2013 | |
| Cells: | Tumor |
| Enzyme(s) | Collagenase Type 1: 0.16% Hyaluronidase: 0.002% Deoxyribonuclease I: 0.006% |
| Medium | DMEM/F12 |
Tumor: Rat | |
| |
| Mehta, N., Lopez, P., Vyas, A. and Schnaar, R.: Gangliosides and Nogo Receptors Independently Mediate Myelin-Associated Glycoprotein Inhibition of Neurite Outgrowth in Different Nerve Cells.
, J Biol Chem 282,
27875-86, 2007 | |
| Cells: | Hippocampal neurons |
| Enzyme(s) | Papain: 0.2% |
| Medium | Hibernate A |
Tumor: Rat | |
| |
| Sharma N, Luo J, Kirschmann DA, O'Malley Y, Robbins ME, Akporiaye ET, Lubaroff DM, Heidger PM, Hendrix MJ: A novel immunological model for the study of prostate cancer
, Cancer Res 59,
2271-6, 1999 | |
| Cells: | Sponge infiltrating cells |
| Enzyme(s) | Collagenase Type 4: 0.15% Deoxyribonuclease I: 0.02% |
| Medium | RPMI 1640 |
Tumor: Rat | |
| |
| Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus
, In Vitro Cell Dev Biol 26,
713, 1990 | |
| Cells: | Epthelial |
| Enzyme(s) | Collagenase Type 3: 0.1% |
| Medium | Eagle's MEM Serum-free |
Tumor: Rat | |
| |
| Brennan, M., Oldberg, A., Hayman, E., and Ruoslahti, E.: Effect of a Proteoglycan Produced by Rat Tumor Cells on Their Adhesion to Fibronectin-Collagen Substrata
, Cancer Res 43,
4302, 1983 | |
| Cells: | Yolk sac tumor |
| Enzyme(s) | Trypsin: 0.01% |
| Medium | DMEM |
Tumor: Rat | |
| |
| Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma
, In Vitro 18,
565, 1982 | |
| Cells: | Epithelial, cancer and tumor |
| Enzyme(s) | Collagenase: 0.1% |
| Medium | Eagles's MEM |
Tumor: Rat | |
| |
| Gazdar, A., Chick, W., Oie, H., Sims, H., King, D., Weir, G., and Lauris, V.: Continuous, Clonal, Insulin-and Somatostatin-Secreting Cell Lines Established from a Transplantable Rat Islet Cell Tumor
, Proc Natl Acad Sci U S A 77 (6),
3519, 1980 | |
| Cells: | Tumor, islet |
| Enzyme(s) | Trypsin: 0.05% |
| Medium | Medium 199 |
Tumor: Rat | |
| |
| Essner, E.: Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands
, Exp Cell Res
7,
430, 1954 | |
| Cells: | Ascites hepatoma |
| Enzyme(s) | Trypsin: 0.1% |
| Medium | Phosphate buffer (see reference) |
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