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Tissue Dissociation References
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Adipose/Fat: Avian
Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges , Methods Mol Biol 1345, 299, 2016
 
Cells:      Amyloid Fibrils
Enzyme(s)     Collagenase Type 3: 0.02%
Deoxyribonuclease I: 0.001%
Medium     50mM Tris


Adipose/Fat: Avian
Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges , Methods Mol Biol 1345, 299, 2016
 
Cells:      Amyloid Fibrils
Enzyme(s)     Collagenase Type 3: 0.02%
Deoxyribonuclease I: 0.001%
Medium     50mM Tris


Adipose/Fat: Bear
  • Bear, Ursus arctos
Gehring, J., Rigano, K., Evans, H., Nelson, O., Robbins, C. and Jansen, H.: A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes. , Cytotechnology 68, 2177-91, 2016
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Adipose/Fat: Bovine
  • Bovine
Yang, Y., Baldwin, R.: Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue , J Dairy Sci 56, 350, 1973
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 40 u/ml
Medium     Krebs-Ringer bicarbonate


Adipose/Fat: Canine
  • 23-33 kg
Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts. , Tissue Eng Part A 19, 1295-302, 2013
 
Cells:      Stromal vascular
Enzyme(s)     Collagenase: see reference
Medium     Media 199E


Adipose/Fat: Canine
  • Canine
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10, 171, 2011
 
Cells:      Renal adipose derived cells
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     DMEM


Adipose/Fat: Canine
  • Canine, 20-25 kg
Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. , J Surg Res 152, 157, 2009
 
Cells:      Adipose stem cells
Enzyme(s)     Collagenase: see reference
Medium     Media-199


Adipose/Fat: Canine
  • Canine
DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species , Am J Physiol 221, 850, 1971
 
Cells:      White fat
Enzyme(s)     Collagenase: 0.05%
Medium     Kreb's Ringer bicarbonate buffer


Adipose/Fat: Equine
  • Equine
Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow. , Vet Surg 37, 713, 2008
 
Cells:      Adipose derived stem cells
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Equine
  • Equine, 1-5 year
Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells. , Vet Surg 36, 613, 2007
 
Cells:      Adipose derived stem cells
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Fish
  • Fish, Atlantic salmon
Todorcevic, M., Vegusdal, A., Gjoen, T., Sundvold, H., Torstensen, B., Kjaer, M. and Ruyter, B.: Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids. , Biochim Biophys Acta 1781, 326, 2008
 
Cells:      Preadipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Adipose/Fat: Gerbil
  • Gerbil of unknown age
    (also rat, hamster, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.: The Brown Fat Cell , Int Rev Cytol 74, 187, 1982
 
Cells:      Brown fat
Enzyme(s)     Collagenase Type 1: 0.10%
Medium     Bicarbonate buffer


Adipose/Fat: Guinea-Pig
  • Guinea-pig, adult
    (also rat, hamster, gerbil, rabbit, lamb)
Nedergaard, J. and Lindberg, O.: The Brown Fat Cell , Int Rev Cytol 74, 187, 1982
 
Cells:      Brown fat
Enzyme(s)     Collagenase Type 1: 0.10%
Medium     Bicarbonate buffer


Adipose/Fat: Hamster
  • Hamster, adult
    (also rat, gerbil, rabbit, lamb, guinea-pig)
Nedergaard, J. and Lindberg, O.: The Brown Fat Cell , Int Rev Cytol 74, 187, 1982
 
Cells:      Brown fat
Enzyme(s)     Collagenase Type 1: 0.10%
Medium     Bicarbonate buffer


Adipose/Fat: Hamster
  • Hamster, 5 week-12 month
DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species , Am J Physiol 221, 850, 1971
 
Cells:      White fat
Enzyme(s)     Collagenase: 0.05%
Medium     Kreb's Ringer bicarbonate buffer


Adipose/Fat: Human
  • Human
Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat. , Curr Protoc Stem Cell Biol 48, e68, 2019
 
Cells:      Adipose derived stem
Enzyme(s)     Collagenase Type 4: 150-300 u/ml
Medium     RPMI-1640


Adipose/Fat: Human
  • Human
Tsurumachi, N., Akita, D., Kano, K., Matsumoto, T., Toriumi, T., Kazama, T., Oki, Y., Saito-Tamura, Y., Tonogi, M., Shimizu, N. and Honda, M.: Effect of Collagenase Concentration on The Isolation of Small Adipocytes from Human Buccal Fat Pad. , J Oral Sci , , 2018
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 2: 0.01-0.5%
Medium     DMEM


Adipose/Fat: Human
  • Human
Wahl, S., Drong, A., Lehne, B., Loh, M., Scott, K., Kunze, S. et al: Epigenome-wide Association Study of Body Mass Index, and the Adverse Outcomes of Adiposity , Nature 541, 81, 2017
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Adipose/Fat: Human
  • Human
Duscher, D., Maan, Z., Luan, A., Aitzetmuller, M., Brett, E., Atashroo, D., Whittam, A., Hu, M., Walmsley, G., Houschyar, K., Schilling, A., Machens, H., Gurtner, G., Longaker, M. and Wan, D.: Ultrasound-Assisted Liposuction Provides a Source for Functional Adipose-Derived Stromal Cells. , Cytotherapy 19, 1491-1500, 2017
 
Cells:      Stromal
Enzyme(s)     Collagenase Type 2: 0.075%
Medium     DMEM


Adipose/Fat: Human
  • Human
Lin, Y., Marin-Argany, M., Dick, C., Redhage, K., Blancas-Mejia, L., Bulur, P., Butler, G., Deeds, M., Madden, B., Williams, A., Wall, J., Dietz, A. and Ramirez-Alvarado, M.: Mesenchymal Stromal Cells Protect Human Cardiomyocytes from Amyloid Fibril Damage , Cytotherapy 19, 1426-1437, 2017
 
Cells:      Mesenchymal stromal
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     PBS


Adipose/Fat: Human
  • Human
Munir, H., Ward, L., Sheriff, L., Kemble, S., Nayar, S., Barone, F., Nash, G. and McGettrick, H.: Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner. , Stem Cells 35, 1636-1646, 2017
 
Cells:      Mesenchymal stem
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM/F-12


Adipose/Fat: Human
  • Human
Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. , Stem Cell Res Ther 8, 69, 2017
 
Cells:      Adipose derived mesenchymal stem
Enzyme(s)     Collagenase animal free: 0.1%
Medium     PBS


Adipose/Fat: Human
  • Human
Szoke, K., Reinisch, A., Ostrup, E., Reinholt, F. and Brinchmann, J.: Autologous Cell Sources in Therapeutic Vasculogenesis: In Vitro and In Vivo Comparison of Endothelial Colony-Forming Cells from Peripheral Blood and Endothelial Cells Isolated from Adipose Tissue. , Cytotherapy 18, 242-52, 2016
 
Cells:      Adipose derived endothelial
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Adipose/Fat: Human
  • Human
Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells , Cytotherapy 18, 371-83, 2016
 
Cells:      Adipose stem
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM/F12


Adipose/Fat: Human
  • Human
Hu, J., Cao, Y., Xie, Y., Wang, H., Fan, Z., Wang, J., Zhang, C., Wang, J., Wu, C. aand Wang, S.: Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice. , Stem Cell Res Ther 7, 130, 2016
 
Cells:      Dental pulp stem
Enzyme(s)     Collagenase animal free: 0.1%
Medium     HBSS


Adipose/Fat: Human
  • Human
Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells. , Cytotherapy 17, 359-68, 2015
 
Cells:      Mesenchymal stromal
Enzyme(s)     Collagenase Type 4: 0.2%
Medium     PBS


Adipose/Fat: Human
  • Human
Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage. , BMC Med Genomics 8, 41, 2015
 
Cells:      Adipose derived stem cells
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     HBSS


Adipose/Fat: Human
  • Human, female
Kinoshita, K., Kuno, S., Ishimine, H., Aoi, N., Mineda, K., Kato, H., Doi, K., Kanayama, K., Feng, J., Mashiko, T., Kurisaki, A. and Yoshimura, K.: Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers. , Stem Cells Transl Med 4, 146, 2015
 
Cells:      Adipose stromal
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     DMEM


Adipose/Fat: Human
  • Human
Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts. , Plast Reconstr Surg 136, 189e-199e, 2015
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Human
  • Human
Esteve, D., Boulet, N., Volat, F., Zakaroff-Girard, A., Ledoux, S., Coupaye, M., Decaunes, P., Belles, C., Gaits-Iacovoni, F., Iacovoni, J., Remaury, A., Castel, B., Ferrara, P., Heymes, C., Lafonta, M. Boloumie, J.: Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells. , Stem Cells 33, 1277-91, 2015
 
Cells:      Adipose stroma-vascular
Enzyme(s)     Neutral Protease: 2.4 u/ml
Collagenase: 250 u/ml
Medium     PBS


Adipose/Fat: Human
  • Human, adult
Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells. , Cytotherapy 16, 915, 2014
 
Cells:      Adipose derived mesenchymal stem
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     see reference


Adipose/Fat: Human
  • Human, adult
Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities. , Cytotherapy 16, 789, 2014
 
Cells:      Adipose derived stem
Enzyme(s)     Collagenase Type 1: 0.15%
Medium     DMEM


Adipose/Fat: Human
  • Human
Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods. , Cytotherapy 16, 1220-8, 2014
 
Cells:      Mesenchymal stromal
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Human
  • Human
Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells. , Tiss Eng 19, 473-8, 2013
 
Cells:      Adipose stromal stem
Enzyme(s)     Collagenase animal free: 200 u/ml
Medium     DMEM/Hams F-12


Adipose/Fat: Human
  • Human
Doi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis. , J Tissue Eng Regen Med 7, 864, 2013
 
Cells:      Stromal vascular fraction
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     PBS


Adipose/Fat: Human
  • Human
Cervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance. , Stem Cells Transl Med 1, 206-20, 2012
 
Cells:      Adipose-derived stem
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Human
  • Human
James, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering. , Stem Cells Transl Med 1, 673, 2012
 
Cells:      Perivascular stem
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Adipose/Fat: Human
  • Human
Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery. , Stem Cells Transl Med 1, 230-6, 2012
 
Cells:      Adipose derived stromal vascular
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Human
  • Human
Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I., Curcio, C., Floris, M., Fiaschetti, V., Floris, R. and Cervell, V.: A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Platelet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction. , Stem Cells Transl Med 1, 341-51, 2012
 
Cells:      Stromal vascular, adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Adipose/Fat: Human
  • Human, adult
Hagman, D., Kuzma, J., Larson, I., Foster-Schubert, K., Kuan, L., Cignarella, A., Geamanu, E., Makar, K., Gottlieb, J. and Kratz, M.: Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing. , J Immunol Methods 386, 50, 2012
 
Cells:      Stromovascular
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS with BSA


Adipose/Fat: Human
  • Human
Naaijkens, B., Niessen, H., Prins, H., Krijnen, P., Kokhuis, T., de Jong, N,, van Hinsbergh, V., Kamp, O., Helder, M., Musters, R., van Dijk, A. and Juffermans, L.: Human Platelet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications. , Cell Tissue Res 348, 119, 2012
 
Cells:      Adipose derived stromal
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Human
  • Human
Wu, I., Nahas, Z., Kimmerling, K., Rosson, G. and Elisseeff, J.: An Injectable Adipose Matrix for Soft-Tissue Reconstruction. , Plast Reconstr Surg 129, 1247, 2012
 
Cells:      Adipose derived stem
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM/F-12


Adipose/Fat: Human
  • Human, male 40-60 years
Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential , Vasc Cell 3, 5, 2011
 
Cells:      Adipose derived stem cells
Enzyme(s)     Collagenase: 0.25%
Deoxyribonuclease I: 0.002%
Medium     PBS


Adipose/Fat: Human
  • Human
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10, 171, 2011
 
Cells:      Renal adipose derived cells
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     DMEM


Adipose/Fat: Human
  • Human
Yang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells. , J Biomed Sci 18, 59, 2011
 
Cells:      Adipose derived mesenchymal
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     L-DMEM


Adipose/Fat: Human
  • Human
Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates. , Methods Mol Biol 702, 17-27, 2011
 
Cells:      Adipose derived stem
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM/Ham's F-12


Adipose/Fat: Human
  • Human
Basu, S., Haghiac, M., Surace, P., Challier, J., Guerre-Millo, M., Singh, K., Waters, T., Minium, J., Presley, L., Catalano, P. and Hauguel-de Mouzon, S.: Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation. , Obesity 19, 476, 2011
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     RPMI


Adipose/Fat: Human
  • Human
Peters, R., Wolf, M., van den Broek, M., Nuvolone, M., Dannenmann, S., Stieger, B., Rapold, R., Konrad, D., Rubin, A., Bertino, J., Aguzzi, A., Heikenwalder, M. and Knuth, A.: Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture. , PLoS ONE 5, e15689, 2010
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     KRB


Adipose/Fat: Human
  • Human
Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering. , J Biomed Mater Res 92, 979, 2010
 
Cells:      Adipose tissue-derived stem
Enzyme(s)     Collagenase Type 2: 1.0%
Medium     DMEM/F12


Adipose/Fat: Human
  • Human
Zimmerlin, L., Donnenberg, V., Pfeifer, M., Meyer, EM, Peault, B., Rubin, JP and Donnenberg, A.: Stromal Vascular Progenitors in Adult Human Adipose Tissue. , Cytometry 77, 22-30, 2010
 
Cells:      Stromal vascular
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     HBSS


Adipose/Fat: Human
  • Human
Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes. , Cytotherapy 12, 538, 2010
 
Cells:      Adipose stromal
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM/Hams F-12


Adipose/Fat: Human
  • Human
Suga, H., Eto, H., Shigeura, T., Inoue, K., Aoi, N., Kato, H., Nishimura, S., Manabe, I., Gonda, K. and Yoshimura, K.: IFATS Collection: Fibroblast Growth Factor-2-Induced Hepatocyte Growth Factor Secretion by Adipose-Derived Stromal Cells Inhibits Postinjury Fibrogenesis Through a c-Jun N-Terminal Kinase-Dependent Mechanism. , Stem Cells 27, 238, 2009
 
Cells:      Adipocytes
Enzyme(s)     Collagenase: 0.075%
Medium     DMEM


Adipose/Fat: Human
  • Human
Cai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function. , Stem Cells 27, 230, 2009
 
Cells:      Adipose stromal
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Adipose/Fat: Human
  • Human
Tan, H., Ramirez, C., Miljkovic, N., Li, H., Rubin, J. and Marra, K.: Thermosensitive Injectable Hyaluronic Acid Hydrogel for Adipose Tissue Engineering. , Biomaterials 30, 6844, 2009
 
Cells:      Adipose tissue-derived stem
Enzyme(s)     Collagenase Type 2: 1.0%
Medium     DMEM/F12


Adipose/Fat: Human
  • Human
Tandon, N., Goh, B., Marsano, A., Chao, P., Montouri-Sorrentino, C., Gimble, J. and Vunjak-Novakovic, G.: Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation. , Conf Proc IEEE Eng Med Biol Soc 2009, 6517-21, 2009
 
Cells:      Adipose derived stem cells
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Adipose/Fat: Human
  • Human
Traktuev, D., Merfeld-Clauss, S., Li, J., Kolonin, M., Arap, W., Pasqualini, R., Johnstone, B., and March, K.: A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks , Circ Res 102, 77, 2008
 
Cells:      Adipose derived stromal cells
Enzyme(s)     Collagenase Type 1: see reference
Medium     DMEM


Adipose/Fat: Human
  • Human
Minana, M., Carbonell-Uberos, F., Mirabet, V., Marin, S. and Encabo, A.: IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue. , Stem Cells 26, 2696, 2008
 
Cells:      Stromal
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     PBS


Adipose/Fat: Human
  • Human
Nie, J., Chang, B., Traktuev, D., Sun, J., March, K., Chan, L., Sage, H., Pasqualini, R., Arap, W. and Kolonin, M.: IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells. , Stem Cells 26, 2735, 2008
 
Cells:      Stromal
Enzyme(s)     Collagenase Type 1: 0.1%
Neutral Protease:
Medium     DMEM


Adipose/Fat: Human
  • Human
Pilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells. , Regen Med 3, 705-15, 2008
 
Cells:      Stem
Enzyme(s)     Collagenase: 280 u/ml
Medium     D-PBS


Adipose/Fat: Human
  • Human
Bujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha: Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat , J Endocrinol 192, 279-88, 2007
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     HBSS


Adipose/Fat: Human
  • Human
Schaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies , Stem Cells 25, 818-27, 2007
 
Cells:      Adipose derived stromal cells
Enzyme(s)     Collagenase Type 1: 0.15%
Medium     DMEM


Adipose/Fat: Human
  • Human
Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors. , J Cell Physiol 212, 702-9, 2007
 
Cells:      Adipose derived stem cells
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM/F12


Adipose/Fat: Human
  • Human
Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK , J Lipid Res 47, 653-64, 2006
 
Cells:      Mesenchymal stem
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Adipose/Fat: Human
  • Human
Koellensperger, E., Von Heimburg, D., Markowicz, M. and Pallua, N.: Human Serum from Platelet-Poor Plasma for the Culture of Primary Human Preadipocytes. , Stem Cells 24, 1218-25, 2006
 
Cells:      Preadipocytes
Enzyme(s)     Collagenase Type 1: 196 u/ml
Medium     M199


Adipose/Fat: Human
  • Human
Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture , Mol Biol Cell 16, 1131-41, 2005
 
Cells:      Adipocytes, stromal vascular
Enzyme(s)     Collagenase: 0.2%
Medium     HBSS


Adipose/Fat: Human
  • Human
Rodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse , J Exp Med 201, 1397-405, 2005
 
Cells:      Multipotent adipose derived stem
Enzyme(s)     Collagenase: 0.2%
Medium     DMEM


Adipose/Fat: Human
  • Human
Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress. , J Biomech Eng 127, 1081, 2005
 
Cells:      Adult stem cells
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Human
  • Human, adult, obese
Seboek, D., Linscheid, P., Zulewski, H., Langer, I., Christ-Crain, M., Keller, U., and Muller, B.: Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation , J Clin Endocrinol Metab 89(10), 4833, 2004
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM/F12


Adipose/Fat: Human
  • Human, adult, female
Quickler, M., Sinha, B., Tomlinson, J., Bujalska, I., Stewart, P., Arlt, W.,: Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5 , J Endocrinol 183, 331, 2004
 
Cells:      Preadipocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     DMEM/F-12


Adipose/Fat: Human
  • Human, adult
Fain JN, Madan AK, Hiler ML, Cheema P, and Bahouth SW.: Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans , Endocrinology 145, 2273, 2004
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.13%
Medium     see reference


Adipose/Fat: Human
  • Human
Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives , Circulation 109, 656-63, 2004
 
Cells:      Stromal vascular, adipocytes
Enzyme(s)     Collagenase: 0.2%
Medium     DMEM/F12


Adipose/Fat: Human
  • Human
Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells , Circulation 110, 349-55, 2004
 
Cells:      Stromal vascular, adipocytes, stem
Enzyme(s)     Collagenase: 300 u/ml
Medium     PBS


Adipose/Fat: Human
  • Human, adult, female, non-obese
Gesta, S., Lolmede, K., Daviaud, D., Berlan, M., Bouloumie, A., Lafontan, M., Valet, P., and Saulnier-Blache, J.: Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression , Horm Metab Res 35, 158, 2003
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     DMEM


Adipose/Fat: Human
  • Human
Patwardhan, R., Tubbs, R., Leonard, R., Kelly, D., Killingsworth, C., Rollins, D., Smith, W., Ideker, R., and Oakes, W.: Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery , Ped Neurosurg 36, 314, 2002
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: see reference
Medium     Saline


Adipose/Fat: Human
  • Human, adult, male and female
McTernan, P., Anderson, L., Anwar, A., Eggo, M., Crocker, J., Barnett, A., Stewart, P., and Kumar, S.: Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences , J Clin Endocrinol Metab 87, 1327, 2002
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     DMEM/F12


Adipose/Fat: Human
  • Human
Zuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells , Mol Biol Cell 13, 4279-95, 2002
 
Cells:      Processed lipoaspirate cells
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     PBS


Adipose/Fat: Human
  • Human
McTernan PG, Anwar A, Eggo MC, Barnett AH, Stewart PM, Kumar S: Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue , Int J Obes Relat Metab Disord 24, 875-81, 2000
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     HBSS


Adipose/Fat: Human
  • Human, adult, male and female
Gottschling-Zelle, H., Birgel, M., Scriba, D., Blum, W., and Hauner, H.: Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1 , Eur J Endocrinol 141 (4), 436, 1999
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM/Ham's F-12


Adipose/Fat: Human
  • Human, adult, male and female
Zhang, H., Kumar, S., Barnett, A., and Eggo, M.: Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake , J Clin Endocrinol Metab 84, 2550, 1999
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM/F12


Adipose/Fat: Human
  • Human, non-diabetic, male
Anderson, O., Gliemann, J., and Gammeltoft: Receptor Binding and Biological Effect of Insulin in Human Adipocytes , Diabetologia 13, 589, 1977
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     Kreb's Ringer bicarbonate buffer


Adipose/Fat: Mouse
  • Mouse
Jones, J., Rabhi, N., Orofino, J., Gamini, R., Perissi, V., Vernochet, C. and Farmer, S.: The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet. , Sci Rep 10, 2380, 2020
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Adipose/Fat: Mouse
  • Mouse
Bowles, A., Strong, A., Wise, R., Thomas, R., Gerstein, B., Dutreil, M., Hunter, R., Gimble, J. and Bunnell, B.: Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis. , Stem Cells 35, 532-544, 2017
 
Cells:      Adipose stromal
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Adipose/Fat: Mouse
  • Mouse
Maria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis. , Cytotherapy 18, 1129-45, 2016
 
Cells:      Adipose mesenchyal stromal
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM


Adipose/Fat: Mouse
  • Mouse
Yao, W., Lay, Y., Kot, A., Liu, R., Zhang, H., Chen, H., Lam, K. and Lane, N.: Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference. , Stem Cells 34, 2587-2600, 2016
 
Cells:      Adipose stromal
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Mouse
  • Mouse
Jang, H., Bhasin, S., Guarneri, T., Serra, C., Schneider, M., Lee, M., Guo, W., Fried, S., Pencina, K. and Jasuja, R.: The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life. , Endocrinology 156, 3737, 2015
 
Cells:      Adipocytes
Enzyme(s)     Collagenase: 0.1%
Medium     Krebs


Adipose/Fat: Mouse
  • Mouse
Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts. , Plast Reconstr Surg 136, 189e-199e, 2015
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Adipose/Fat: Mouse
  • Mouse, 20 week
Kondo, T, Toyoshima, Y., Ishii, Y and Kyuwa, S.: Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice. , Exp Anim 62, 319, 2013
 
Cells:      Stromal vascular
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     RPMI-1640


Adipose/Fat: Mouse
  • Mouse
Takahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair. , Stem Cells Transl Med 1, 803, 2012
 
Cells:      Adipose derived stem
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Adipose/Fat: Mouse
  • Mouse
Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells. , Blood 115, 957, 2010
 
Cells:      Stem and progenitor
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     HBSS


Adipose/Fat: Mouse
  • Mouse, 3 week
De Matteis, R., Zingaretti, M., Murano, I., Vitali, A., Frontini, A., Giannulis, I., Barbatelli, G., Marcucci, F., Bordicchia, M., Sarzani, R., Raviola, E. and Cinti, S.: In Vivo Physiological Transdifferentiation of Adult Adipose Cells. , Stem Cells 27, 2761, 2009
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     HBSS


Adipose/Fat: Mouse
  • Mouse
Wong, K., Szeto, F, Zhang, W., Ye, H., Kong, J., Zhang, Z., Sun, X. and Li, Y: Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins. , Am J Physiol/Endo 296, 820, 2009
 
Cells:      White adipocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Adipose/Fat: Mouse
  • Mouse
Thangarajah, H., Vial, I., Chang, E., El-Ftesi, S., Januszyk, M., Chang, E., Paterno, J., Neofytou, E., Longaker, M. and Gurtner, G.: IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia. , Stem Cells 27, 266, 2009
 
Cells:      Adipose stromal
Enzyme(s)     Collagenase Type 2: 0.075%
Medium     DMEM


Adipose/Fat: Mouse
  • Mouse
Cho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model. , Stem Cells 27, 259-65, 2009
 
Cells:      Adipose tissue-derived stem
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     Modified Eagles


Adipose/Fat: Mouse
  • Mouse, C57Bl/6J
Aoyagi T, Shimba S, Tezuka M: Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes, J Health Sci 51, 21, 2005
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.15%
Medium     DMEM/F12


Adipose/Fat: Mouse
  • Mouse, 3-6 day and 2-3 month
Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T: Adipose-derived adult stromal cells heal critical-size mouse calvarial defects , Nat Biotechnol 22, 560-7, 2004
 
Cells:      Adipose-derived stromal cells
Enzyme(s)     Collagenase Type 2: 0.075%
Medium     PBS


Adipose/Fat: Mouse
  • Mouse
Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives , Circulation 109, 656-63, 2004
 
Cells:      Stromal vascular, adipocytes
Enzyme(s)     Collagenase: 0.2%
Medium     DMEM/F12


Adipose/Fat: Mouse
  • Mouse, C57BL/6J and FVB, male, 8-9 week
Ruan, H., Zarnowski, MJ., Cushman, S., and Lodish, H.: Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes , J Biol Chem 278, 47585, 2003
 
Cells:      Adipocytes
Enzyme(s)     Collagenase: see reference
Medium     DMEM


Adipose/Fat: Mouse
  • Mouse, both sexes
Nadler, S., Stoehr, J., Rabaglia, M., Schueler, K., Birnbaum, M., and Attie, A.: Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice , Am J Physiol/Endo 281, E1249, 2001
 
Cells:      Adipocytes
Enzyme(s)     Collagenase: 0.05%
Medium     Krebs-Ringer Phosphate HEPES (KRPH)


Adipose/Fat: Mouse
  • Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male
Launder, T., Gegen, N., Knedler, A., and Harbeck, R.: The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue , J Immunol Methods 102, 45, 1987
 
Cells:      Vascular endothelial
Enzyme(s)     Collagenase: 0.2%
Medium     PBS


Adipose/Fat: Porcine
  • Porcine, female, <1 year
Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells. , Cells Tissues Organs 188, 251, 2008
 
Cells:      Adipose mesenchymal stem
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Adipose/Fat: Porcine
  • Porcine, crossbred, male 1-4 day
Ramsay, T.G.: Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes , J Anim Sci 81, 3008, 2003
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     DMEM/F12


Adipose/Fat: Porcine
  • Porcine, crossbred
Liang, W. and Mills, S.: Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues. , J Anim Sci 80, 963-70, 2002
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.067%
Medium     Krebs-Ringer


Adipose/Fat: Porcine
  • Porcine, 8-9 week
Ding, S., McNeel, R., and Mersmann, H.: Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo , Comp Biochem Physiol B 123, 307, 1999
 
Cells:      Adipose
Enzyme(s)     Collagenase Type 1: 300 u/ml
Medium     HEPES


Adipose/Fat: Porcine
  • Porcine, neonatal
Wang Y, Fried SK, Petersen RN, Schoknecht PA: Somatotropin regulates adipose tissue metabolism in neonatal swine , J Nutr 129, 139-45, 1999
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     Krebs-Ringer bicarbonate albumin


Adipose/Fat: Porcine
  • Porcine, crossbred, 1-3 day
Suryawan, A., Swanson, L., and Hu, C.: Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture , J Anim Sci 75, 105, 1997
 
Cells:      Adipose, Stromal-vascular
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     DMEM/F12


Adipose/Fat: Rat
  • Rat, SD, 2-3 month
Dayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells , Cytotherapy 19, 937-946, 2017
 
Cells:      Mesenchymal stromal
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Adipose/Fat: Rat
  • Rat, 9 week
Ohta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury. , Cytotherapy 19, 839-848, 2017
 
Cells:      Adipose stromal
Enzyme(s)     Collagenase: 0.1%
Medium     DMEM


Adipose/Fat: Rat
  • Rat
Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model. , Cytotherapy 17, 543-59, 2015
 
Cells:      Mesenchymal stem
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     DMEM


Adipose/Fat: Rat
  • Rat, SD, 5 month
Veronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells. , Cytotherapy 17, 215-23, 2015
 
Cells:      Mesenchymal stromal
Enzyme(s)     Collagenase Type 2: 0.075%
Medium     DMEM


Adipose/Fat: Rat
  • Rat
Ogasawara, J., Izawa, T., Sakurai, T., Shirato, K., Ishibashi, Y., Ohira, Y., Ishida, H., Ohno, H. and Kizaki, T.: Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes. , Biochem Biophys Res Commun 464, 348-53, 2015
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     Krebs-Ringer


Adipose/Fat: Rat
  • Rat, adult
Leblanc, A., Nguyen, Q., Touroo, J., Aird, A., Chang, R., Ng, C., Hoying, J. and Williams, S.: Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct. , Stem Cells Transl Med 2, 896-905, 2013
 
Cells:      Stromal vascular
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     PBS


Adipose/Fat: Rat
  • Rat, male, 300g
Thompson, A., Nunez, M., Davidson, R., Horm, T., Schnittker, K., Hart, M., Suarez, A. and Tsao, T.: Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue. , J Lipid Res 53, 2797, 2012
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 2: 0.33%
Medium     RPMI 1640


Adipose/Fat: Rat
  • Rat, Lewis, male
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10, 171, 2011
 
Cells:      Renal adipose derived cells
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     DMEM


Adipose/Fat: Rat
  • Rat, SD, neonatal
Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose. , J Biomed Biotechnol 2010, 104296, 2010
 
Cells:      Brown adipocytes
Enzyme(s)     Collagenase Type 4: 0.1%
Neutral Protease: 0.1%
Trypsin: 0.05%
Medium     PBS


Adipose/Fat: Rat
  • Rat
Wei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats. , Stem Cells 27, 478, 2009
 
Cells:      Adipose derived stem
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Adipose/Fat: Rat
  • Rat
Tomiyama, K., Murase, N., Stolz, D., Toyokawa, H., O'Donnell, D., Smith, D., Dudas, J., Rubin, J. and Marra, K.: Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue. , Stem Cells 26, 330, 2008
 
Cells:      Adipose tissue-derived stem
Enzyme(s)     Collagenase Type 2: 0.075%
Medium     DMEM


Adipose/Fat: Rat
  • Rat, Wistar, 4 week
Aoki, S., Toda, S., Sakemi, T., and Sugihara, H.: Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro , Cell Struct Funct 28, 55, 2003
 
Cells:      Adipocytes
Enzyme(s)     Collagenase: 0.2%
Medium     Ham's F12


Adipose/Fat: Rat
  • Rat, SD
Mora, S., Yang, C., Ryder, J., Boeglin, D and Pessin, J: The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency , Endocrinology 142, 1999, 2001
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     KRHB


Adipose/Fat: Rat
  • Rat, SD, male, 4-7 weeks
Omatsu-Kanbe, M., and Matsuura, H.: Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes , J Physiol 521 (3), 601, 1999
 
Cells:      Brown adipocytes
Enzyme(s)     Deoxyribonuclease I: 0.5%
Medium     DMEM


Adipose/Fat: Rat
  • Rat
Serrero, G: Primary Culture in Defined Medium of Adipocyte Precursors,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 11B:6.1, 1995
 
Cells:      Adipocytes
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM /F-12


Adipose/Fat: Rat
  • Rat, SD, male, 130-160 g
Charron, M.J. and Kahn, B.B.: Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo , J Biol Chem 265, 7994, 1990
 
Cells:      Adipose
Epididymal fat pads
Enzyme(s)     Collagenase: 0.3%
Medium     Kreb's-Ringer bicarbonate buffer modified


Adipose/Fat: Rat
  • Rat, 3 day
Gaben-Cogneville, A., Poussin, B., Chamblier, M., Forgue-Fafitte, M., and Rosselin, G.: Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture , Biochim Biophys Acta 968, 231, 1988
 
Cells:      Preadipocytes
Enzyme(s)     Collagenase Type 3: 0.10%
Medium     Parker Medium 199


Adipose/Fat: Rat
  • Rat, SD, various weights and ages
Woodward, Julie A. and Saggerson, E.: Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline , Biochem J 238, 395, 1986
 
Cells:      Brown adipocytes
Interscapular & cervical depots
Enzyme(s)     Collagenase: 0.2%
Soybean Trypsin Inhibitor: 0.3%
Medium     Krebs Ringer bicarbonate buffer


Adipose/Fat: Rat
  • Rat, CD, male, 150-200 g
Pessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P.: ß-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes , J Biol Chem 258, 7386, 1983
 
Cells:      Adipocytes
Epididymal fat pads
Enzyme(s)     Collagenase: 0.1%
Medium     Krebs Ringer bicarbonate buffer


Adipose/Fat: Rat
  • Rat, Wistar, albino, male, 100-140 g
Green, A. and Newsholme, E.: Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites , Biochem J 180, 365, 1979
 
Cells:      Adipocytes
Epididymal-fat pads
Enzyme(s)     Collagenase: 0.3%
Medium     Kreb's Ringer


Adipose/Fat: Rat
  • Rat, Fischer, 344, male, 9 - 13 week
Stiles, J.W., Francendese, A.A. and Masoro, E.J.: Influence of Age on Size and Number of Fat Cells in the Epididymal Depot , Am J Physiol 229, 1561, 1975
 
Cells:      White fat
Enzyme(s)     Collagenase: 0.3%
Medium     Kreb's Ringer bicarbonate buffer


Adipose/Fat: Rat
  • Rat, Wistar, male, 5 week-16 month
DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species , Am J Physiol 221, 850, 1971
 
Cells:      White fat
Enzyme(s)     Collagenase: 0.05%
Medium     Kreb's Ringer bicarbonate buffer


Adipose/Fat: Rat
  • Rat (CFE), albino, female
Fain, J., Reed, N., and Saperstein, R.: Isolation and Metabolism of Brown Fat Cells , J Biol Chem 242, 1887, 1967
 
Cells:      Brown fat
Enzyme(s)     Collagenase Type 1: 0.10%
Medium     Bicarbonate buffer


Adipose/Fat: Rat
  • Rat, SD, male, 160-210 g
Rodbell, M.: Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis , J Biol Chem 239 (2), 375, 1964
 
Cells:      Fat
Enzyme(s)     Collagenase: 0.3%
Medium     Albumin-bicarbonate buffer


Adipose/Fat: Squirrel
  • Squirrel
McFarlane, S., Mathers, K. and Staples, J.: Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator. , Am J Physiol Regul Integr Comp Physiol 312, R434-R442, 2017
 
Cells:      Brown adipocytes
Enzyme(s)     Collagenase Type 2: 0.16%
Medium     Krebs-Ringer phosphate


Adrenal: Bovine
  • Bovine
Dominguez, N., Rodri­guez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells. , Methods Mol Biol 846, 223, 2012
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
Medium     DMEM/F12


Adrenal: Bovine
  • Bovine, 6 month
Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B: Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core , Cell Transplant 15, 121-33, 2006
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.125%
Medium     Locke's solution


Adrenal: Bovine
  • Bovine
Hahm, S., Chen, Y., Vinson, C. and Eiden, L.: A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization. , Mol Pharmacol 64, 1503, 2003
 
Cells:      Chromaffin cells
Enzyme(s)     Collagenase: 0.1%
Deoxyribonuclease I: 30 u/ml
Medium     DMEM


Adrenal: Bovine
  • Bovine
Ortega, J., Sagen, J., and Pappas, G.: Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS , Cell Transplant 1, 33, 1992
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.2%
Medium     Locke's solution


Adrenal: Bovine
  • Bovine
Zhu, J., Li, W., Toews, M., and Hexum, T.: Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process , J Pharmacol Exp Ther 263 (3), 1479, 1992
 
Cells:      Chromaffin
Enzyme(s)     Deoxyribonuclease I: 30 u/mg
Medium     HEPES


Adrenal: Bovine
  • Bovine
    (also rat)
Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26, 335, 1990
 
Cells:      Heart
Adrenal chromaffin
Paraneurons
Enzyme(s)     Trypsin: 0.06%
Medium     25mM HEPES buffered Locke's solution, CMF


Adrenal: Bovine
  • Bovine
Dahmer, M., Hart, P., and Perlman, R.: Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells , J Neurochem 54 (3), 931, 1990
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.25 %
Medium     DMEM


Adrenal: Bovine
  • Bovine
Higgins, L., and Berg, D.: Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells , J Neurosci 7 (6), 1792, 1987
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.2%
Deoxyribonuclease I: 0.015%
Medium     Locke's solution


Adrenal: Bovine
  • Bovine
Aunis, D., Rotllan, P., and Miras-Portugal, M.: Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture, Neurochem Int 7, 89, 1985
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.05%
Medium     Locke's solution, CMF


Adrenal: Bovine
  • Bovine
Almazan, G., Aunis, D., Garcia, A., Montiel, C., Nicolas, G., and Sanchez-Garcia, P.: Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells , Br J Biomed Sci 81, 599, 1984
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.15%
Medium     Kreb's, CMF


Adrenal: Bovine
  • Bovine
Pollard, H., Pazoles, C., Creutz, C., Scott, J., Zinder, O., and Hotchkiss, A.: An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells , J Biol Chem 259, 1114, 1984
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.1%
Medium     (see reference)


Adrenal: Bovine
  • Bovine
Cena, V., Garcia, A., Montiel, C., and Sanchez-Garcia, P.: Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells , Br J Pharmacol 81, 119, 1984
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     CF Kreb's


Adrenal: Bovine
  • Bovine
Waymire, J., Bennett, W., Boehme, R., Hankins, L., Gilmer-Waymire, K., and Haycock, J.: Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture , J Neurosci Methods 7, 329, 1983
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.025%
Medium     HBSS, modified


Adrenal: Bovine
  • Bovine
Knight, D. and Baker, P.: Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells , Q J Exp Physiol 68, 123, 1983
 
Cells:      Medulla
Enzyme(s)     Hyaluronidase: 0.2%
Medium     Saline w/BSA 0.5%


Adrenal: Bovine
  • Bovine
Greenberg, A. and Zinder, O.: alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells , Cell Tissue Res 226, 655, 1982
 
Cells:      Medulla
Enzyme(s)     Collagenase: 0.2%
Medium     Krebs-Ringer bicarbonate buffer, CMF


Adrenal: Bovine
  • Bovine
Wilson, S.P., and Viveros, O.H.: Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium , Exp Cell Res 133, 159, 1981
 
Cells:      Chromaffin
Enzyme(s)     Deoxyribonuclease I: 15 µg/ml
Medium     Medium A
(see reference)


Adrenal: Bovine
  • Bovine
Baker, P., and Knight., D: Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells , Phil Trans R Soc Lond 296, 83, 1981
 
Cells:      Medulla
Enzyme(s)     Protease: 0.2%
Medium     Saline


Adrenal: Bovine
  • Bovine
Trifaro, J.M., and Lee, R.W.: Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures , Neuroscience 5, 1533, 1980
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.05%
Medium     Locke's solution, CMF


Adrenal: Bovine
  • Bovine, adult
    (also rat, Hanover-Wistar, young; guinea-pig, newborn)
Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro , Adv Biochem Psychopharmacol 255, 51, 1980
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.5%
Collagenase: 0.5%
Medium     HBSS


Adrenal: Bovine
  • Bovine
Kumakura, K., Karoum, F., Guidotti, A., and Costa, E.: Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells , Nature 283, 489, 1980
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.25%
Medium     F-12 medium


Adrenal: Bovine
  • Bovine
Kilpatrick, D., Ledbetter, F., Carson, K., Kirshner, A., Slepetis, R., and Kirshner, N.: Stability of Bovine Adrenal Medulla Cells in Culture , J Neurochem 35 (3), 679, 1980
 
Cells:      Medulla
Enzyme(s)     Collagenase: 0.05%
Medium     Locke's solution, CF


Adrenal: Bovine
  • Bovine, adult
Unsicker, K., and Griesser, G.: Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures , Neuroscience 5, 1445, 1980
 
Cells:      Medulla
Enzyme(s)     Collagenase Type 1: 0.5%
Medium     HBSS


Adrenal: Bovine
  • Bovine
Aunis, D., Guerold, B., Bader, M-F., and Cieselski-Treska, J.: Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells , Neuroscience 5, 2261, 1980
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.05%
Medium     DMEM


Adrenal: Bovine
  • Bovine
Hersey, R., and DiStefano, V.: Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells , J Pharmacol Exp Ther 209 (1), 147, 1979
 
Cells:      Medullary
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     HEPES


Adrenal: Bovine
  • Bovine
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
 
Cells:      Foreskin
Enzyme(s)     Collagenase: 0.5%
Medium     Dulbecco's MEM w/10% calf serum


Adrenal: Bovine
  • Bovine
Fenwick, E., Fajdiga, P., Howe, N., and Livett, B.: Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell , J Cell Biol 76, 12, 1978
 
Cells:      Medulla
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     Kreb's, CF


Adrenal: Bovine
  • Bovine
Brooks, J.C.: The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion , Endocrinology 101, 1369, 1977
 
Cells:      Chromaffin
Enzyme(s)     Hyaluronidase: 0.2%
Medium     HEPES, CF


Adrenal: Bovine
  • Bovine, male
Schneider, A., Herz, R., and Rosenheck, K.: Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla , Proc Natl Acad Sci U S A 74, 5036, 1977
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     Kreb's Ringer bicarbonate buffer, CF


Adrenal: Guinea-Pig
  • Guinea-pig, 500-700 g
Role, L.W., Leeman, S.E., and Perlman, R.L.: Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla , Neurochem Int 6, 1813, 1981
 
Cells:      Chromaffin
Medulla
Enzyme(s)     Collagenase:
Medium     BSS
(see reference)


Adrenal: Guinea-Pig
  • Guinea-pig
    (also rat, Hanover-Wistar, young; newborn; cattle)
Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro , Adv Biochem Psychopharmacol 255, 51, 1980
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.5%
Collagenase: 0.5%
Medium     HBSS


Adrenal: Guinea-Pig
  • Guinea-pig
Hochman, J., and Perlman, R.L.: Catecholamine Secretion by Isolated Adrenal Cells , Biochim Biophys Acta 421, 168, 1976
 
Cells:      Adrenal
Chromaffin
Enzyme(s)     Collagenase: 0.05%-0.20%
Medium     Kreb's-Ringer bicarb glucose buffer, CF


Adrenal: Hamster
  • Hamster (Mesocricetus auratus) 100-150 g
Liang, B.T., and Perlman, R.L.: Catecholamine Secretion by Hamster Adrenal Cells , J Neurochem 32, 927, 1979
 
Cells:      Adrenal
Chromaffin
Enzyme(s)     Hyaluronidase: 0.20%
Medium     Kreb's Ringer bicarbonate buffer


Adrenal: Human
  • Human
Santana, M., Chung, K., Vukicevic, V., Rosmaninho-Salgado, J., Kanczkowski, W., Cortez, V., Hackmann, K., Bastos, C., Mota, A., Schrock, E., Bornstein, S., Cavadas, C. and Ehrhart-Bornstein, M.: Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla. , Stem Cells Transl Med 1, 783, 2012
 
Cells:      Adrenal medulla progenitor
Enzyme(s)     Collagenase: 0.2%
Medium     DMEM/F-12


Adrenal: Human
  • Human
Jeon, Y., Baek, W., Chung, S., Shin, N., Kim, H., and Lee, S.: Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model. , Korean J Anesthesiol 60, 357, 2011
 
Cells:      Chromaffin cells
Enzyme(s)     Collagenase: 0.2%
Medium     Locke's solution


Adrenal: Human
  • Human
Caroccia, B., Fassina, A., Seccia, T., Recarti, C., Petrelli, L., Belloni, A., Pelizzo, M. and Rossi, G.: Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method. , Endocrinology 151, 1375, 2010
 
Cells:      Adrenocortical
Enzyme(s)     Collagenase Type 1: 0.2%
Deoxyribonuclease I: 0.01%
Medium     Krebs Ringer


Adrenal: Human
  • Human, adult
Tischler, A., DeLellis, R., Bailes, B., Nunnemacher, G.,Carabba, V., and Wolfe, H.: Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells , Lab Invest 43, 399, 1980
 
Cells:      Chromaffin
Enzyme(s)     Trypsin: 0.25%
Medium     Eagle's MEM


Adrenal: Human
  • Human, adult and child
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
 
Cells:      Foreskin
Enzyme(s)     Collagenase: 0.5%
Medium     Dulbecco's MEM w/10% calf serum


Adrenal: Mouse
  • Mouse
Dominguez, N., Rodri­guez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells. , Methods Mol Biol 846, 223, 2012
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
Medium     DMEM/F12


Adrenal: Mouse
  • Mouse, 8-10 week
Kolski-Andreaco, A., Cai, H., Currle, D., Chandy, K. and Chow, R.: Mouse Adrenal Chromaffin Cell Isolation. , J Vis Exp 2, 129, 2007
 
Cells:      Chromaffin
Enzyme(s)     Papain: 40 u/ml
Medium     DMEM


Adrenal: Mouse
  • Mouse, embryonic
Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang: The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells , J Neurosci 25, 10546-55, 2005
 
Cells:      Chromafin cells
Enzyme(s)     Papain: 20-25 u/ml
Medium     DMEM


Adrenal: Ovine
  • Ovine, adult and fetal
Valego, N. and Rose, J.: A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells. , Reprod Sci 17, 477, 2010
 
Cells:      Adrenocortical
Enzyme(s)     Collagenase Type 1: 0.4%
Medium     DMEM/Ham's F12


Adrenal: Ovine
  • Ovine, fetal
Valego, N., Su, Y., Carey, L., Young, S., Tatter, S., Wang, J. and Rose, J.: Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression. , Am J Physiol Regul Integr Comp Physiol 289, R410, 2005
 
Cells:      Adrenocortical
Enzyme(s)     Collagenase Type 1: 0.4%
Medium     DMEM/Ham's F12


Adrenal: Ovine
  • Ovine, adult
Keating, D., Rychkov, G., Adams, M., Holgert, H., McMillen, I.C. and Roberts, M.: Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. , J Physiol 555, 489, 2004
 
Cells:      Chromaffin cells
Enzyme(s)     Collagenase Type 2: 0.2%
Deoxyribonuclease I: 100 u/ml
Medium     Locke's solution


Adrenal: Ovine
  • Ovine, 3 year
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J: The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep , J Endocrinol 162, 215-25, 1999
 
Cells:      Anterior pituitary
Enzyme(s)     Trypsin: 2.5%
Deoxyribonuclease I: 0.004%
Medium     DMEM


Adrenal: Rat
  • Rat
Dominguez, N., Rodri­guez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells. , Methods Mol Biol 846, 223, 2012
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.1-0.2%
Deoxyribonuclease I: 0.003-0.015%
Hyaluronidase: 0.015%
Medium     DMEM/F12


Adrenal: Rat
  • Rat
Gilabert, J, Montalvo, G, and Artalejo A.: Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays, Nat Protoc , 294, 2006
 
Cells:      Chromaffin
Enzyme(s)     Collagenase Type 1: 0.26%
Deoxyribonuclease I: 0.015%
Hyaluronidase: 0.015%
Medium     HBSS


Adrenal: Rat
  • Rat, SD
Gilabert, J: Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture,Cell Biology of the Chromaffin Cell,Borges, R. and Gandia, L., Instituto Teofilo Hernando, , 2004
 
Cells:      Chromaffin cells
Enzyme(s)     Collagenase Type 1: 0.26%
Deoxyribonuclease I: 0.015%
Hyaluronidase: 0.015%
Medium     HBSS


Adrenal: Rat
  • Rat, SD, male
Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel: An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells , Am J Physiol Regul Integr Comp Physiol 284, R1631-5, 2003
 
Cells:      Zona fasciculata/reticularis
Enzyme(s)     Collagenase: 0.4%
Medium     Krebs-HEPES


Adrenal: Rat
  • Rat, Wistar, newborn
Zhang L, Castell A, Avila E, Drucker-ColĂ­n R, Escobar A: Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons , J Neuropathol Exp Neurol 59, 170-4, 2000
 
Cells:      Chromaffin cells
Enzyme(s)     Collagenase Type 1: 0.025%
Deoxyribonuclease I: 0.0015%
Medium     DMEM


Adrenal: Rat
  • Rat, SD, male
Sayed, S., Whitehouse, B., and Jones, P.: Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis , J Endocrinol 154, 449, 1997
 
Cells:      ZG
ZFR
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     Kreb's


Adrenal: Rat
  • Rat, Fischer, male, 10-16 weeks
Roskelley, C.D. and Auersperg, N.: Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture , In Vitro Cell Dev Biol 26, 493, 1990
 
Cells:      Adrenocortical
Enzyme(s)     Deoxyribonuclease I: 0.005%
Medium     BSS


Adrenal: Rat
  • Rat, male, 120-160 g,
    Rat, SD, male, 400-450 g
Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis , In Vitro Cell Dev Biol 26, 24, 1990
 
Cells:      Leydig
Adrenal
Enzyme(s)     Collagenase Type 2: 0.03% (adrenal)
Medium     Krebs Ringer bicarbonate buffer


Adrenal: Rat
  • Rat, SD, 2-4 day old
    (also bovine)
Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26, 335, 1990
 
Cells:      Heart
Adrenal chromaffin
Paraneurons
Enzyme(s)     Trypsin: 0.06%
Medium     25mM HEPES buffered Locke's solution, CMF


Adrenal: Rat
  • Rat, Long-Evans, female, 150-200 g
Payet, N., Deziel, Y., and Lehoux, J.-G.: Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture , J Steroid Biochem 20, 449, 1984
 
Cells:      Glomerulosa
Enzyme(s)     Collagenase: 0.2%
Medium     MEM-d-Val


Adrenal: Rat
  • Rat, Fischer, male, 1-10 months
Leonard, R.K., Auersperg, N., and Parkes, C.O.: Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells , In Vitro 19, 46, 1983
 
Cells:      Adrenocortical
Enzyme(s)     Deoxyribonuclease I: 0.005%
Medium     BSS


Adrenal: Rat
  • Rat, SD, male, 400-450 g
Li, C.H., Ng, T.B., and Cheng, C.H.K.: Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells , Int J Pept Protein Res 19, 361, 1982
 
Cells:      Decapular
Capsular
Glomerulosa
Enzyme(s)     Deoxyribonuclease I: 0.01%
Medium     Medium 199


Adrenal: Rat
  • Rat
Englert, D.F.: An Optical Study of Isolated Rat Adrenal Chromaffin Cells , Exp Cell Res 125, 369, 1980
 
Cells:      Chromaffin
Enzyme(s)     Trypsin: 0.10%
Medium     Ham's F-12 w/HEPES


Adrenal: Rat
  • Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle)
Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro , Adv Biochem Psychopharmacol 255, 51, 1980
 
Cells:      Chromaffin
Enzyme(s)     Collagenase: 0.5%
Collagenase: 0.5%
Medium     HBSS


Adrenal: Rat
  • Rat , SD, female, 200 g
Braley, L., Williams, G., and Bradwin, G.: The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells , Endocrinology 106 (1), 50, 1980
 
Cells:      Glomerulosa
Enzyme(s)     Deoxyribonuclease I: 0.05%
Medium     Kreb's


Adrenal: Rat
  • Rat
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
 
Cells:      Foreskin
Enzyme(s)     Collagenase: 0.5%
Medium     Dulbecco's MEM w/10% calf serum


Adrenal: Rat
  • Rat, Wistar-Hanover, 7-12 day
Unsicker, K., Krisch, B., Otten, U., and Thoenen, H.: Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids , Proc Natl Acad Sci U S A 75 (7), 3498, 1978
 
Cells:      Medullary
Enzyme(s)     Trypsin: 0.125%
Medium     HBSS


Adrenal: Rat
  • Rat, SD, male
Barofsky, A., Feinstein, M., and Halkerston, I.: Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands , Exp Cell Res 79, 263, 1973
 
Cells:      Cortical
Enzyme(s)     Trypsin: 0.25%
Medium     Kreb's Ringer bicarbonate buffer


Adrenal: Rat
  • Rat, Holtzman, male, 180-250 g
Kloppenborg, P., Island, D., Liddle, G., Michelakis, A., and Nicholson, W.: A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism , Endocrinology 82, 1053, 1968
 
Cells:      Adrenal
Enzyme(s)     Collagenase Type 1: 0.5%
Medium     Kreb's Ringer bicarbonate buffer


Bone: Bovine
  • Bovine
Buschmann, M., Gluzband, Y., Grodzinsky, A., and Hunziker, E.: Mechanical Compression Modulates Matrix Biosynthesis in Chondrocyte/Agarose Culture , J Cell Sci 108, 1497, 1995
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.4%
Medium     DMEM


Bone: Chicken
  • Chick, day old
Gay, C., Lloyd, Q., and Gilman, V.: Characteristics and Culture of Osteoblasts Derived From Avian Long Bone , In Vitro Cell Dev Biol 30A, 379, 1994
 
Cells:      Osteoblasts
Enzyme(s)     Trypsin: 0.03%
Medium     DMEM


Bone: Chicken
  • Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus)
Rosselot, G., Reginato, A.M., and Leach, R.M.: Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes , In Vitro Cell Dev Biol 28A, 235, 1992
 
Cells:      Chondrocytes
Enzyme(s)     Trypsin: 0.25%
Medium     Ham's F12


Bone: Chicken
  • Chick, embryo
Schiltz, J.R., Mayne R., and Holtzer, H.: The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture, Differentiation 1, 97, 1973
 
Cells:      Vertebrae chondroblasts
Enzyme(s)     Trypsin: 0.25%
Medium     Simm's, CMF


Bone: Human
  • Human
Agarwal, S., Loder, S., Sorkin, M., Li, S., Shrestha, S., Zhao, B., Mishina, Y., James, A. and Levi, B.: Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. , Stem Cells 34, 1692-701, 2016
 
Cells:      Bone-cartilage-stromal
Enzyme(s)     Collagenase Type 2: 0.75%
Medium     HBSS


Bone: Human
  • Human
Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts. , Bonekey Rep 3, 585, 2014
 
Cells:      Osteoblasts
Enzyme(s)     Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
Medium     DMEM


Bone: Human
  • Human
Kode, A.,Manavalan1, J.,Mosialou1, I., Bhagat G., Rathinam, C, Luo, N et al.: Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts , Nature 506, 240, 2014
 
Cells:      Osteoblasts
Enzyme(s)     Collagenase Type 3: 0.2%
Medium     MEM


Bone: Human
  • Human, 22-73 year
Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ: Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute , Cells Tissues Organs 179, 158-69, 2005
 
Cells:      Osteoblasts
Enzyme(s)     Trypsin: 0.5%
Medium     Basal Medium


Bone: Human
  • Human, 60+ year
Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage , J Bone Miner Res 14, 362, 1999
 
Cells:      Bone Cells, Osteoblasts
Enzyme(s)     Collagenase Type 2: 200-250 u/ml
Medium     DMEM


Bone: Human
  • Human
Meikle, M., Boyd, S., Hembry, R., Compston, J., Croucher, P., and Reynolds, J.: Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines , J Cell Sci 103, 1093, 1992
 
Cells:      Osteoblasts
Enzyme(s)     Trypsin: 0.1%
Medium     DMEM


Bone: Human
  • Human
Fedarko, N.S., Termine, J.D., Young, M.F. and Robey, P.G.: Temporal Regulation of Hyaluronan and Proteoglycan Metabolism by Human Bone Cells in Vitro , J Biol Chem 265, 12200, 1990
 
Cells:      Osteoblasts
Enzyme(s)     Collagenase Type 4: 250 u/ml
Medium     DMEM


Bone: Mouse
  • Mouse
Mohrin, D., Koukos, G., Alabdulaaly, L., Paw, J., Hake, K., Kolumam, G., Bouxsein, M., Baron, R., Kutskova, Y., Freund, A., Liu, J., Zavala-Solorio, J., Bhargava, S., Maxwell, Trumble, J., Brito, A., Hu, D. and Brooks, D.: Inhibition of Longevity Regulator PAPP-A Modulates Tissue Homeostasis via Restraint of Mesenchymal Stromal Cells. ,Aging Cell Vol. 20,, , e13313, 2021
 
Cells:      Bone and bone marrow stromal
Enzyme(s)     Stemxyme 1: 0.1%
Neutral Protease: 0.1%
Medium     PBS


Bone: Mouse
  • Mouse
Baryawno, N., Przybylski, D., Kowalczyk, M., Kfoury, Y., Severe, N., Gustafsson, K., Kokkaliaris, K., Mercier, F., Tabaka, M., Hofree, M., Dionne, D., Papazian, A., Lee, D., Ashenberg, O. and Subramanian, A.: A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia. , Cell 177, 1915-1932.e16, 2019
 
Cells:      Bone and bone marrow stroma
Enzyme(s)     Stemxyme 1: 0.1%
Neutral Protease: 0.1%
Medium     Media 199


Bone: Mouse
  • Mouse
Wang, W., Majihail, G., Lui, C. and Zhou, L.: Osteoblast Sorting and Intracellular Staining of CXCL12. , Bio Protoc 8, , 2018
 
Cells:      Osteoblast
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     HBSS


Bone: Mouse
  • Mouse
Agarwal, S., Loder, S., Sorkin, M., Li, S., Shrestha, S., Zhao, B., Mishina, Y., James, A. and Levi, B.: Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification. , Stem Cells 34, 1692-701, 2016
 
Cells:      Bone-cartilage-stromal
Enzyme(s)     Collagenase Type 2: 0.75%
Medium     HBSS


Bone: Mouse
  • Mouse
Igarashi, H., Akahoshi, N., Ohto-Nakanishi, T., Yasuda, D. and Ishii, S.: The Lysophosphatidic Acid Receptor LPA4 Regulates Hematopoiesis-Supporting Activity of Bone Marrow Stromal Cells. , Sci Rep 5, 11410, 2015
 
Cells:      Bone stromal
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     DMEM


Bone: Mouse
  • Mouse
Lee, D., Choung, H., Kim, H., Gronostajski, R., Yang, Y., Ryoo, H., Lee, Z., Kim, H., Cho, E. and Park, J.: NFI-C Regulates Osteoblast Differentiation Via Control of Osterix Expression. , Stem Cells 32, 2467-79, 2014
 
Cells:      Osteoblasts
Enzyme(s)     Collagenase Type 1: 1.0%
Neutral Protease: 1.6%
Medium     MEM


Bone: Mouse
  • Mouse, 2-5 day
Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts. , Bonekey Rep 3, 585, 2014
 
Cells:      Osteoblasts
Enzyme(s)     Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
Medium     DMEM


Bone: Mouse
  • Mouse
Kode, A.,Manavalan1, J.,Mosialou1, I., Bhagat G., Rathinam, C, Luo, N et al.: Leukemogenesis Induced by an Activating β-catenin Mutation in Osteoblasts , Nature 506, 240, 2014
 
Cells:      Osteoblasts
Enzyme(s)     Collagenase Type 3: 0.2%
Medium     MEM


Bone: Mouse
  • Mouse, 4-22 month
Stern, A., Stern, M., Van Dyke, M., Jahn, K., Prideaux, M. and Bonewald, L.: Isolation and Culture of Primary Osteocytes from the Long Bones of Skeletally Mature and Aged Mice. , Biotechniques 52, 361, 2012
 
Cells:      Osteocytes
Enzyme(s)     Collagenase Type 1: 300 u/ml
Medium     MEM


Bone: Mouse
  • Mouse
Nakamura, Y., Arai, F., Iwasaki, H., Hosokawa, K., Kobayashi, I., Gomei, Y., Matsumoto, Y., Yoshihara, H. and Suda, T.: Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells. , Blood 116, 1422, 2010
 
Cells:      Endosteal cells
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     DMEM


Bone: Mouse
  • Mouse
Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H. and Matsuzaki, Y.: Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow. , J Exp Med 206, 2483-96, 2009
 
Cells:      Bone marrow
Enzyme(s)     Collagenase: 0.2%
Medium     DMEM


Bone: Mouse
  • Mouse, male, 6-8 week old
Sakai, E., Miyamoto, H., Okamoto, K., Kato, Y., Yamamoto, K., and Sakai, H.: Characterization of Phagosomal Subpopulations Along Endocytic Routes in Osteoclasts and Macrophages , J. Biochem. 130, 823, 2001
 
Cells:      Osteoclasts
Enzyme(s)     Collagenase Type 3: 0.1%
Medium     DMEM


Bone: Mouse
  • Mouse, 3-5 day, 6-8 week
Chen X., Qian H., Neff L., Satomura K., and Horowitz M.: Thy-1 antigen Expression by Cells in the Osteoblast Lineage , J Bone Miner Res 14, 362, 1999
 
Cells:      Bone Cells, Osteoblasts
Enzyme(s)     Collagenase Type 2: 200-250 u/ml
Medium     DMEM


Bone: Mouse
  • Mouse, BALB/c
Takanashi, H., Matsuishi, T., and Yoshizato, K.: Establishment and Characterization of Stromal Cell Lines That Support Differentiation of Murine Hematopoietic Blast Cells into Osteoblast-like Cells , In Vitro Cell Dev Biol 30A, 384, 1994
 
Cells:      Osteoblast-like Cells
Stromal Cell Lines
Hematopoietic Blast Cells
Enzyme(s)     Trypsin: 0.1%
Medium     Eagle's MEM


Bone: Mouse
  • Mouse, Swiss-Webster
Chen,T. and Feldman,D.: Regulation of 1,25-Dihydroxyvitamin D3 Receptors in Cultured Mouse Bone Cells , J Biol Chem 256, 5561, 1981
 
Cells:      Neonatal bone
Enzyme(s)     Collagenase Type 2: 0.20%
Medium     Tris-buffered saline


Bone: Rat
  • Rat, 2 day
Jeon, J., Lee, M. and Yang, H.: Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation. , Biomater Res 22, 4, 2018
 
Cells:      Osteoblasts
Enzyme(s)     Collagenase Type 2: 0.1%
Trypsin: 0.25%
Medium     DMEM


Bone: Rat
  • Rat, Wistar, 250g
Jacobs, F., Gijsen, H., Van de Vyver, M. and Ferris, W.: Vanadate Impedes Adipogenesis in Mesenchymal Stem Cells Derived from Different Depots within Bone. , Front Endocrinol 7, 108, 2016
 
Cells:      Proximal femur stem
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     HBSS


Bone: Rat
  • Rat, 2-3 day
Taylor, S., Shah, M. and Orriss, I.: Generation of Rodent and Human Osteoblasts. , Bonekey Rep 3, 585, 2014
 
Cells:      Osteoblasts
Enzyme(s)     Collagenase Type 2: 0.2%
Trypsin: 0.25%-1.0%
Medium     DMEM


Bone: Rat
  • Rat, fetal, 21 days of gestation
Owen, T., Aronow, M., Shalhoub, V., Barone, L., Wilming, L., Tassinari, M., Kennedy, M., Pockwinse, S., Lian, J., and Stein, G.: Progressive Development of the Rat Oseoblast Phenotype In Vitro: Reciprocal Relationships in Expression of Genes Associated with Osteoblast Proliferation and Differentiation During Formation of the Bone Extracellular Matrix , J Cell Physiol 143, 420, 1990
 
Cells:      Calvaria
Enzyme(s)     Collagenase: 0.2%
Medium     MEM


Bone: Rat
  • Rat, newborn
Ernst, M., and Froesch, E.: Osteoblastlike Cells in a Serum-Free Methylcellulose Medium Form Colonies: Effects of Insulin and Insulinlike Growth Factor I , Calcif Tissue Int 40, 27, 1987
 
Cells:      Osteoblastlike cells
Enzyme(s)     Collagenase Type 2: 0.3%
Medium     MEM


Bone: Rat
  • Rat, fetus, 17-21 day
Peck, W., Birge, S., and Fedak, S.: Bone Cells: Biochemical and Biological Studies After Enzymatic Isolation , Science 146, 1476, 1964
 
Cells:      Calvaria
Enzyme(s)     Collagenase: 0.01%-0.6%
Medium     Tris-buffered saline


Brain: Bovine
  • Bovine
Kanda, T., Yoshino, H., Ariga, T., Yamawaki, M., and Yu, R.: Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy , J Cell Biol 126 (1), 235, 1994
 
Cells:      Microvascular endothelial
Enzyme(s)     Neutral Protease: 0.005%
Medium     Medium 199


Brain: Bovine
  • Bovine
Wolburg H, Neuhaus J, Kniesel U, Krauss B, Schmid EM, Ocalan M, Farrell C, Risau W: Modulation of tight junction structure in blood-brain barrier endothelial cells. Effects of tissue culture, second messengers and cocultured astrocytes , J Cell Sci 107, 1347, 1994
 
Cells:      Brain endothelial cells
Enzyme(s)     Collagenase Type 2: 0.35%
Medium     DMEM


Brain: Bovine
  • Bovine
Miller, D., Audus, K., and Borchardt, R.: Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier, J Tiss Cul Meth 14, 217, 1992
 
Cells:      Endothelial
Enzyme(s)     Neutral Protease: 0.125%
Medium     MEM


Brain: Bovine
  • Bovine, adult
Machi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries , In Vitro Cell Dev Biol 26, 291, 1990
 
Cells:      Cerebral artery
Endothelial
Enzyme(s)     Collagenase: 0.2%
Medium     HBSS


Brain: Bovine
  • Bovine
Estrada, C., Bready, J., Berliner, J., and Cancilla, P.: Choline Uptake by Cerebral Capillary Endothelial Cells in Culture , J Neurochem 54, 1467, 1990
 
Cells:      Capillary endothelial
Enzyme(s)     Collagenase: 0.1%
Medium     DMEM


Brain: Bovine
  • Bovine
Audus, K., and Borchardt, R.: Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism, Pharm Res 3 (2), 81, 1986
 
Cells:      Endothelial
Enzyme(s)     Neutral Protease: 0.5%
Medium     MEM


Brain: Bovine
  • Bovine
Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain , In Vitro Cell Dev Biol 21, 172, 1985
 
Cells:      Endothelial
Brain arteries
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     Dulbecco's PBS


Brain: Bovine
  • Calf
    (also lamb)
Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain , J Biol Chem 253, 1592, 1978
 
Cells:      Oligodendroglia
Neural
Enzyme(s)     Trypsin: 0.1%
Medium     (see reference)


Brain: Guinea-Pig
  • Guinea-pig, 200-400 g
Kay, A.R., and Wong, R.K.S.: Isolation of Neurons Suitable for Patch-Clamping from Adult Mammalian Central Nervous Systems , J Neurosci Methods 16, 227, 1986
 
Cells:      Neurons
Enzyme(s)     Trypsin: 0.06-0.08%
Medium     PIPES saline


Brain: Human
  • Human
Mizee, M., Miedema, S., van der Poel, M., Adelia , S., van Strien, M., Melief, J., Smolders, J., Hendrickx, D., Heutinck, K., Hamann, J. and Huitinga, I.: Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. , Acta Neuropathol 5, 16, 2017
 
Cells:      Microglia
Enzyme(s)     Collagenase Type 1: 300 u/ml
Trypsin: 0.125%
Medium     DMEM


Brain: Human
  • Human, adult
Spaethling, J., Na, Y., Lee, J., Ulyanova, A., Baltuch, G., Bell, T., Brem, S., Chen, H., Dueck, H., Fisher, S., Garcia, M., Khaladkar, M., Kung, D., Lucas, T., O'Rourke, D. and Stefanik, D.: Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. , Cell Rep 18, 791-803, 2017
 
Cells:      Neuronal
Enzyme(s)     Papain: 20 u/ml
Medium     Neurobasal


Brain: Human
  • Human, fetal and mature
Zhang, Y., Sloan, S., Clarke, L., Caneda, C., Plaza, C., Blumenthal, P., Vogel, H., Steinberg, G., Edwards, M., Li, G., Duncan, J., Cheshier, S., Shuer, L., Chang, E., Grant, G., Gephart, M. and Barres, B.: Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. , Neuron 89, 37-53, 2016
 
Cells:      Astrocytes and neurons
Enzyme(s)     Papain: 7.5-20 u/ml
Medium     RPMI


Brain: Human
  • Human
Rustenhoven, J., Park Thomas, I., Schweder, P., Scotter, J., Correia, J., Smith, A., Gibbons, H., Oldfield, R., Bergin, P., Mee, E., Faull, R., Curtis, M., Scott, G. and Dragunow, M.: Isolation of Highly Enriched Primary Human Microglia for Functional Studies. , Sci Rep 6, 19371, 2016
 
Cells:      Microglia
Enzyme(s)     Neutral Protease: 10 u/ml
Papain: 2.5 u/ml
Medium     Hibernate A


Brain: Human
  • Human, 25-81 yr
Volovitz, I, Shapira, N., Ezer, H., Gafni, A., Lustgarten, M., Alter, T., Ben-Horin, I., Barzilai, O., Shahar, T., Kanner, A., Fried, I., Veshchev, I., Grossman, R. and Ram, Z,: A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. , BMC Neurosci 17, 30, 2016
 
Cells:      Brain tumor
Enzyme(s)     Neutral Protease: 0.11 u/ml
Collagenase Type 4: 0.05%
Hyaluronidase: 1,000 u/ml
Deoxyribonuclease I: 5 u/ml
Medium     HBSS


Brain: Human
  • Human
Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges , Methods Mol Biol 1345, 299, 2016
 
Cells:      Amyloid Fibrils
Enzyme(s)     Collagenase Type 3: 0.02%
Deoxyribonuclease I: 0.001%
Medium     50mM Tris


Brain: Human
Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.: Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. , Neuro Oncol 13, 70-83, 2011
 
Cells:      Brain tumor
Enzyme(s)     Collagenase Type 1: 0.04%
Hyaluronidase: 0.01%
Deoxyribonuclease I: 0.02%
Neutral Protease: 0.008%
Medium     DMEM/F12


Brain: Human
  • Human
Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.: Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. ,Neuro Oncol Vol. 11,, , 109, 2009
 
Cells:      Tumor
Enzyme(s)     Collagenase Type 4: 0.1%
Hyaluronidase: 0.07%
Deoxyribonuclease I: 0.04%
Medium     see reference


Brain: Human
  • Human
Klegeris Andis, McGeer PatrickL: Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity , Glia 51, 56-64, 2005
 
Cells:      Microglia
Enzyme(s)     Trypsin: 0.25%
Deoxyribonuclease I: .005%
Medium     DMEM/F12


Brain: Human
  • Human
Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels , Brain Res Bull 21, 785, 1988
 
Cells:      Microvessels
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     DMEM


Brain: Human
  • Human
Roher, A.E., Palmer, K.C., Chau, V., and Ball, M.J.: Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein , J Cell Biol 107, 2703, 1988
 
Cells:      Neuronal
Enzyme(s)     Deoxyribonuclease I: 10 µg/ml
Medium     Tris-HCl, 50 mM, CaCl2, 2 mM


Brain: Human
  • Human, 15-54 years
Vinters, H.V., Reave, S., Costello, P., Girvin, J.P., and Moore, S.A.: Isolation and Culture of Cells Derived From Human Cerebral Microvessels , Cell Tissue Res 249, 657, 1987
 
Cells:      Microvessels
Enzyme(s)     Collagenase: 0.1%
Medium     Serum-free modified Lewis medium


Brain: Insect
  • Drosophilia
Hadzic, T., Park, D., Abruzzi, K., Yang, L., Trigg, J., Rohs, R., Rosbash, M. and Taghert, P.: Genome-Wide Features of Neuroendocrine Regulation in Drosophila by the Basic Helix-Loop-Helix Transcription Factor DIMMED. , Nucleic Acids Res 43, 2199-215, 2015
 
Cells:      Neurons
Enzyme(s)     Papain: 50 u/ml
Medium     Modified Dissecting Saline


Brain: Insect
  • Drosophila
Gu, H. and O'Dowd, D.: Whole Cell Recordings from Brain of Adult Drosophila. , J Vis Exp 6, 248, 2007
 
Cells:      Neurons
Enzyme(s)     Papain: 20 u/ml
Medium     Saline


Brain: Insect
  • Drosophila
Sicaeros, B. Campusano, J. and O'Dowd, D.: Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae. , J Vis Exp 4, 200, 2007
 
Cells:      Neurons
Enzyme(s)     Papain: 4 u/ml
Medium     DMEM


Brain: Insect
  • Drosophilia
Gu, H. and O'Dowd, D.: Cholinergic Synaptic Transmission in Adult Drosophila Kenyon Cells in Situ. , J Neurosci 26, 265-72, 2006
 
Cells:      Kenyon
Enzyme(s)     Papain: 20 u/ml
Medium     Recording saline


Brain: Monkey
  • Monkey, rhesus
Marcondes MC, Burudi EM, Huitron-Resendiz S, Sanchez-Alavez M, Watry D, Zandonatti M, Henriksen SJ, Fox HS: Highly activated CD8(+) T cells in the brain correlate with early central nervous system dysfunction in simian immunodeficiency virus infection , J Immunol 167, 5429-38, 2001
 
Cells:      Brain cells
Enzyme(s)     Collagenase Type 2: 500 u/ml
Deoxyribonuclease I: 28 u/ml
Medium     HBSS


Brain: Mouse
  • Adult and neonatal
Calvo, Belen, Rubio, F., Fernandez, M. and Tranque, P.: Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. ,IBRO Rep Vol. 8,, , 36-47, 2020
 
Cells:      Microglia, astrocytes, lymphocytes
Enzyme(s)     Collagenase Type 2: 120 u/ml
Papain: 20 u/ml
Neutral Protease: 1.2 u/ml
Deoxyribonuclease I: 20 u/ml
Medium     EBSS


Brain: Mouse
  • Mouse
Kramer, D., Risso, D., Kosillo, ., Ngai, J. and Bateup, H.: Combinatorial Expression of Grp and Neurod6 Defines Dopamine Neuron Populations with Distinct Projection Patterns and Disease Vulnerability ,eNeuro Vol. 5,, , 1, 2018
 
Cells:      Neurons
Enzyme(s)     Papain: 10 u/ml
Deoxyribonuclease I: 10 u/ml
Medium     Artificial CSF


Brain: Mouse
  • Mouse, adult
Swartzlander, D., Propson, N., Roy, E., Saito, T., Saido, T., Wang, B. and Zheng, H.: Concurrent Cell Type-Specific Isolation and Profiling of Mouse Brains in Inflammation and Alzheimer's Disease. , JCI Insight 3, , 2018
 
Cells:      Astrocytes, microglia, endothelia, oligodendrocytes
Enzyme(s)     Papain: 20 u/ml
Collagenase Type 1: 0.01%
Medium     HBSS


Brain: Mouse
  • Mouse, fetal and mature
Zhang, Y., Sloan, S., Clarke, L., Caneda, C., Plaza, C., Blumenthal, P., Vogel, H., Steinberg, G., Edwards, M., Li, G., Duncan, J., Cheshier, S., Shuer, L., Chang, E., Grant, G., Gephart, M. and Barres, B.: Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse. , Neuron 89, 37-53, 2016
 
Cells:      Astrocytes and neurons
Enzyme(s)     Papain: 7.5-20 u/ml
Medium     RPMI


Brain: Mouse
  • Mouse
Baruch, K., Rosenzweig, N., Kertser, A., Deczkowska, A., Sharif, A., Spinrad, A., Tsitsou-Kampeli, A., Sarel, A., Cahalon, L. and Schwartz, M.: Breaking Immune Tolerance by Targeting Foxp3(+) Regulatory T Cells Mitigates Alzheimer's Disease Pathology. , Nat Commun 6, 7967, 2015
 
Cells:      Chloroid plexus
Enzyme(s)     Collagenase Type 4: 400 u/ml
Medium     PBS


Brain: Mouse
  • Mouse, 3 week
Luo,Y.,Coskun, V., Liang, A.,Yu, J., Cheng, L.,Ge, W., Shi, Z., Zhang, K., Li, C., Lin, H., Luo, D., Wang, J., Lin, C., Dai, Z., Zhu, H., Zhang, J., Liu, J., Liu, H.,deVellis, J., Horvath, S., Sun, Y. and Li, S.: Different Patterns of Molecular Forms of Somatostatin are Released by the Rat Median Eminence and Hypothalamus. , Cell 161, 1175, 2015
 
Cells:      Neural
Enzyme(s)     Papain: 20 u/ml
Medium     PBS


Brain: Mouse
  • Mouse
Ryg-Cornejo, V., Ioannidis, L. and Hansen, D.: Isolation and Analysis of Brain-Sequestered Leukocytes from Plasmodium Berghei ANKA-Infected Mice. , J Vis Exp 71, e50112, 2013
 
Cells:      Brain-sequestered leukocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Deoxyribonuclease I: 2 u/ml
Medium     RPMI


Brain: Mouse
  • Mouse
Shah. G., Price, T., Banks, W., Morofuji, Y., Kovac, A., Ercal, N., Sorenson, C., Shin, E., Sheibani, N.: Pharmacological Inhibition of Mitochondrial Carbonic Anhydrases Protects Mouse Cerebral Pericytes from High Glucose-Induced Oxidative Stress and Apoptosis. , J Pharmacol Exp Ther 344, 637-45, 2013
 
Cells:      Cerebral pericytes
Enzyme(s)     Collagenase Type 2: 0.1%
Deoxyribonuclease I: 30 u/ml
Medium     DMEM


Brain: Mouse
  • Mouse, P13-15
Haseleu, J., Anlauf, E., Blaess, S., Endl, E. and Derouiche, A.: Studying Subcellular Detail in Fixed Astrocytes: Dissociation of Morphologically Intact Glial Cells (DIMIGs). , Front Cell Neurosci 7, 54, 2013
 
Cells:      Glial
Enzyme(s)     Papain: 20 u/ml
Deoxyribonuclease I: 0.0005%
Medium     EBSS


Brain: Mouse
  • Mouse, P0-P15
Foo, L.: Purification of Rat and Mouse Astrocytes by Immunopanning. , Cold Spring Harb. Protoc. 2013, 421-32, 2013
 
Cells:      Astrocytes
Enzyme(s)     Papain: 5 u/ml
Deoxyribonuclease I: 0.004%
Medium     DPBS


Brain: Mouse
  • Mouse, adult
Saxena, A., Wagatsuma, A., Noro, Y., Kuji, T., Asaka-Oba, A., Watahiki, A., Gurnot, C., Fagiolini, M., Hensch, T. and Carninci, P.: Trehalose-Enhanced Isolation of Neuronal Sub-Types from Adult Mouse Brain. , Biotechniques 52, 381, 2012
 
Cells:      Neurons
Enzyme(s)     PDS kit: with modifications
Medium     DMEM/F12


Brain: Mouse
  • Mouse, 1-2 day
O'Meara, R., Ryan, S., Colognato, H. and Kothary, R.: Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues. , J Vis Exp 54, 3324, 2011
 
Cells:      Oligodendrocytes, dorsal root ganglia
Enzyme(s)     Papain: 0.15%
Deoxyribonuclease I: 0.006%
Medium     DMEM


Brain: Mouse
  • Mouse, 6-12 week
Chung, W., Farley, J., Swenson, A., Barnard, J., Hamilton, G., Chiposi, R. and Drummond, H.: Extracellular Acidosis Activates ASIC-like Channels in Freshly Isolated Cerebral Artery Smooth Muscle Cells. , Am J Physiol Cell Physiol 298, C1198, 2010
 
Cells:      Vascular smooth muscle cells
Enzyme(s)     Papain: 0.05%
Collagenase Type 4: 0.15%
Elastase: 0.05%
Medium     PBS


Brain: Mouse
  • Mouse, 4-6 day
Lee, H., Greene, L., Mason, C. and Manzini, M.: Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons. , J Vis Exp 23, 990, 2009
 
Cells:      Neurons
Enzyme(s)     PDS kit: with modifications
Medium     HBSS


Brain: Mouse
  • Mouse, postnatal
Sher, F., Rossler, R., Brouwer, N., Balasubramaniyan, V., Boddeke, E. and Copray, S.: Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. , Stem Cells 26, 2875, 2008
 
Cells:      Astrocytes
Enzyme(s)     Trypsin: 0.25%
Deoxyribonuclease I: 1,000 u/ml
Medium     HBSS


Brain: Mouse
  • Mouse, neonatal
Fasano, C., Thibault, D. and Trudeau, L.: Culture of Postnatal Mesencephalic Dopamine Neurons on an Astrocyte Monolayer ,Current Protocols in Neuroscience Vol. 44,, , 3.21.1, 2008
 
Cells:      Neurons
Enzyme(s)     Papain: 20 u/ml
Medium     Neurobasal


Brain: Mouse
  • Mouse, embryonic and postnatal
Hilgenberg, L. and Smith, M.: Preparation of Dissociated Mouse Cortical Neuron Cultures. , J Vis Exp 10, 562, 2007
 
Cells:      Cortical neurons
Enzyme(s)     Papain: 4-10 u/ml
Medium     Neurobasal


Brain: Mouse
  • Mouse
Klein C, Butt SJ, Machold RP, Johnson JE, and Fishell G.: Cerebellum- and forebrain-derived stem cells possess intrinsic regional character , Development 132, 4497, 2005
 
Cells:      Neurosperes
Enzyme(s)     PDS kit: per instructions
Medium     DMEM/F12


Brain: Mouse
  • Mouse, 1 day
Seaberg, R., Smukler, S. and Van der Kooy, D.: Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain. , Dev Biol 278, 71, 2005
 
Cells:      Neural progenitor
Enzyme(s)     PDS kit: per instructions
Medium     see reference


Brain: Mouse
  • Mouse
Oliver, T., Read, T., Kessler, J., Mehmeti, A., Wells, J., Huynh, T., Lin, S. and Wechsler-Reya, R.: Loss of Patched and Disruption of Granule Cell Development in a Pre-Neoplastic Stage of Medulloblastoma. , Development 132, 2425, 2005
 
Cells:      Granule cell precursors, pre-neoplastic and tumor cells
Enzyme(s)     Papain: 10 u/ml
Deoxyribonuclease I: 250 u/ml
Medium     Neurobasal/B27


Brain: Mouse
  • Mouse
Hernandez, F., Perez, M., Lucas, J., Mata, A., Bhat, R. and Avila, J.: Glycogen Synthase Kinase-3 plays a Crucial Role in Tau Exon 10 Splicing and Intranuclear Distribution of SC35. Implications for Alzheimer's Disease. , J Biol Chem 279, 3801, 2004
 
Cells:      Cortical neurons
Enzyme(s)     PDS kit: per instructions
Medium     Neurobasal


Brain: Mouse
  • Mouse, 3 day
Nishioku T, Hashimoto K, Yamashita K, Liou SY, Kagamiishi Y, Maegawa H, Katsube N, Peters C, von Figura K, Saftig P, Katunuma N, Yamamoto K, Nakanishi H: Involvement of cathepsin E in exogenous antigen processing in primary cultured murine microglia , J Biol Chem 277, 4816, 2002
 
Cells:      Microglia
Enzyme(s)     Papain: 90 u/ml
Deoxyribonuclease I: 2000 u/ml
Medium     Eagle's MEM


Brain: Mouse
  • Mouse, SD, 8-12 week
O'Donnell SL, Frederick TJ, Krady JK, Vannucci SJ, Wood TL: IGF-I and microglia/macrophage proliferation in the ischemic mouse brain , Glia 39, 85, 2002
 
Cells:      Microglia
Enzyme(s)     Trypsin: 0.125%
Collagenase Type 2: 0.01%
Deoxyribonuclease I: .005%
Medium     RPMI-1640


Brain: Mouse
  • Mouse, 2-5 day
Smeyne Michelle, Smeyne RichardJ: Method for culturing postnatal substantia nigra as an in vitro model of experimental Parkinson's disease , Brain Res Brain Res Protoc 9, 105-11, 2002
 
Cells:      Postnal substantia nigra
Enzyme(s)     PDS kit: per instructions
Medium     see reference


Brain: Mouse
  • Mouse, embryonic
Estivill-Torrus, G., Pearson, H., Van Heyningen, V., Price, D. and Rashbass, P.: Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors. , Development 129, 455, 2002
 
Cells:      Cortical progenitors
Enzyme(s)     PDS kit: per instructions
Medium     Serum free medium


Brain: Mouse
  • Mouse, 1 day
Martin-Aparicio, E., Yamamoto, A., Hernandez, F., Hen, R., Avila, J. and Lucas, J.: Proteasomal-Dependent Aggregate Reversal and Absence of Cell Death in a Conditional Mouse Model of Huntington's Disease. , J Neurosci 21, 8772, 2001
 
Cells:      Neurons and glia
Enzyme(s)     PDS kit: per instructions
Medium     Neurobasal


Brain: Mouse
  • Mouse
Miyazawa K, Himi T, Garcia V, Yamagishi H, Sato S, and Ishizaki Y.: A role for p27/Kip1 in the control of cerebellar granule cell precursor proliferation , J Neurosci 20, 5756, 2000
 
Cells:      Cerebellar granule cell precursors
Enzyme(s)     Papain: 0.435%
Deoxyribonuclease I: 0.05%
Medium     EBSS


Brain: Mouse
  • Mouse
Lim, D, and Alvarez-Buylla, A.: Interaction Between Astrocytes and Adult Subventricular Zone Precursors Stimulates Neurogenesis , Proc Natl Acad Sci U S A 96, 7526, 1999
 
Cells:      Astrocytes
Enzyme(s)     Trypsin: 0.25%
Medium     DMEM


Brain: Mouse
  • Mouse, newborn
Jun K, Choi G, Yang SG, Choi KY, Kim H, Chan GC, Storm DR, Albert C, Mayr GW, Lee CJ, Shin HS: Enhanced hippocampal CA1 LTP but normal spatial learning in inositol 1,4,5-trisphosphate 3-kinase(A)-deficient mice , Learn Mem 5, 317-30, 1998
 
Cells:      Hippocampal cells
Enzyme(s)     Papain: 10 u/ml
Medium     DMKM


Brain: Mouse
  • Mouse, SWR or CF1, 1-3 months
Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.: A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells , Brain Res 503, 326, 1989
 
Cells:      Papillae, taste receptor
Enzyme(s)     Pronase E: 0.15%
Medium     Carbonate-Phosphate buffer
(see reference)


Brain: Mouse
  • Mouse, 0-30 day
Shrier, B., Wilson, S., and Nirenberg, M.: Cultured Cell Systems and Methods for Neurobiology , Vol. 32,, , 765, 1974
 
Cells:      Neural
Enzyme(s)     Trypsin NF 1:250: 0.25%
Medium     BSS


Brain: Ovine
  • Ovine
Reddy, R., Amodei, R., Estill, C., Stormshak, F., Meaker, M. and Roselli, C.: Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. , PLoS ONE 10, e0129521, 2015
 
Cells:      Neurons
Enzyme(s)     Papain: 0.2%
Deoxyribonuclease I: 0.1%
Medium     Hibernate


Brain: Ovine
  • Ovine
Lepore G, Gadau S, Mura A, Zedda M, Farina V: Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury. , Eur J Histochem 53, e28, 2009
 
Cells:      Neurons
Enzyme(s)     PDS kit: per instructions
Medium     DMEM


Brain: Ovine
  • Lamb
    (also calf)
Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain , J Biol Chem 253, 1592, 1978
 
Cells:      Oligodendroglia
Neural
Enzyme(s)     Trypsin: 0.1%
Medium     (see reference)


Brain: Porcine
  • Mini pigs, Yucatan (Susscrofa Yucatan), 4-6 months
Robinson, D.H., Kang,Y., Deschner, S.H., and Nielsen, T.B.: Morphologic Plasticity and Periodicity : Porcine Cerebral Microvascular Cells in Culture , In Vitro Cell Dev Biol 26, 169, 1990
 
Cells:      Microvascular
Enzyme(s)     Collagenase: 0.1%
Medium     HBSS


Brain: Rat
  • Rat, SD, 1-2 day
Kurachi, M., Mikuni, M. and Ishizaki, Y.: Extracellular Vesicles from Vascular Endothelial Cells Promote Survival, Proliferation and Motility of Oligodendrocyte Precursor Cells. , PLoS ONE 11, e0159158, 2016
 
Cells:      Oligodendrocyte precursor cells
Enzyme(s)     Papain: 16.5 u/ml
Medium     HBSS


Brain: Rat
  • Rat, SD, E18
Folts, C., Scott-Hewitt, N., Proschel, C., Mayer-Proschel, M. and Noble, M.: Lysosomal Re-acidification Prevents Lysosphingolipid-Induced Lysosomal Impairment and Cellular Toxicity. , PLoS Biol 14, e1002583, 2016
 
Cells:      Neurons
Enzyme(s)     Papain: 20 u/ml
Medium     HBSS


Brain: Rat
  • Rat, P0-P15
Foo, L.: Purification of Rat and Mouse Astrocytes by Immunopanning. , Cold Spring Harb. Protoc. 2013, 421-32, 2013
 
Cells:      Astrocytes
Enzyme(s)     Papain: 5 u/ml
Deoxyribonuclease I: 0.004%
Medium     DPBS


Brain: Rat
  • Rat, SD, neonatal
Kaiser, O., Aliuos, P., Wissel, K., Lenarz, T., Werner, D., Reuter, G., Kral, A. and Warnecke, A.: Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi After Digestion with Papain. , PLoS ONE 8, e80490, 2013
 
Cells:      Neurons
Enzyme(s)     Papain: 20 u/ml
Medium     Neuro medium


Brain: Rat
  • Rat, SD, 19-21 day
Cao, R., Li, A., Cho, H., Lee, B. and Obrietan, K.: Mammalian Target of Rapamycin Signaling Modulates Photic Entrainment of the Suprachiasmatic Circadian Clock. , J Neurosci 30, 6302, 2010
 
Cells:      Suprachiasmatic nucleus neurons
Enzyme(s)     Papain: 100 u/ml
Medium     MEM


Brain: Rat
  • Rat, Fisher, 7-21 month
Chen, N., Newcomb, J., Garbuzova-Davis, S., Davis Sanberg, C., Sanberg, P. and Willing, A.: Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro. , Aging Dis 1, 173, 2010
 
Cells:      Hippocampal neurons
Enzyme(s)     Papain: 0.2%
Medium     Hibernate A


Brain: Rat
  • Rat, SD, 7 day
Tanaka, S., Shaikh, I., Chiocca, E. and Saeki, Y.: The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development. , PLoS ONE 4, e5922, 2009
 
Cells:      Cerebellar granule neurons
Enzyme(s)     PDS kit: per instructions
Medium     PBS


Brain: Rat
  • Rat, neonatal
Shigetomi, E. and Khakh, B.: Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes. , J Vis Exp 26, 1142, 2009
 
Cells:      Astrocytes
Enzyme(s)     Papain: 20 u/ml
Medium     EBSS


Brain: Rat
  • Rat, neonatal
Richler Esther, Chaumont Severine, Shigetomi Eiji, Sagasti Alvaro, Khakh Baljit S: Tracking transmitter-gated P2X cation channel activation in vitro and in vivo , Nat Methods 5, 87-93, 2008
 
Cells:      Hippocampal neurons
Enzyme(s)     Papain: 10 u/ml
Medium     EBSS


Brain: Rat
  • Rat, SD
Akanda, N., Tofighi, R., Brask, J., Tamm, C., Elinder, F. and Ceccatelli, S.: Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells. , Cell Cycle 7, 3225, 2008
 
Cells:      Hippocampal
Enzyme(s)     Trypsin: 0.1%
Medium     Neurobasal


Brain: Rat
  • Rat, neonatal
Peptan, I., Hong, L. and Evans, C.: Multiple Differentiation Potentials of Neonatal Dura Mater-Derived Cells. , Neurosurgery 60, 346, 2007
 
Cells:      Dura mater
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     DMEM


Brain: Rat
  • Rat, E18
Jekabsons MB, Nicholls DG: Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation , Cell Death Differ 13, 1595-610, 2006
 
Cells:      Hippocampal neurons
Enzyme(s)     Papain: 0.2%
Medium     Hibernate


Brain: Rat
  • Rat, Wistar, 1-3 day
Velasco Myrian, Garci­a Esperanza, Onetti Carlos G: Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons , Cell Mol Neurobiol 26, 307-19, 2006
 
Cells:      Hippocampal neurons
Enzyme(s)     Trypsin: 0.05%
Medium     DMEM


Brain: Rat
  • Rat, E19
Khakh Baljit S, Fisher James A, Nashmi Raad, Bowser David N, Lester Henry A: An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy , J Neurosci 25, 6911-20, 2005
 
Cells:      Hippocampal neuron
Enzyme(s)     Papain: 10 u/ml
Medium     MEM


Brain: Rat
  • Rat, Wistar, 14 day
Mizoguchi Y, Kanematsu T, Hirata M, Nabekura J: A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex , J Biol Chem 278, 44097, 2003
 
Cells:      Visual cortical
Enzyme(s)     PDS kit: per instructions
Medium     EBSS


Brain: Rat
  • Rat, Wistar, male
Floris S, Van den Born J, van der Pol SM, Dijkstra CD, De Vries HE: Heparan sulfate proteoglycans modulate monocyte migration across cerebral endothelium , J Neuropathol Exp Neurol 62, 780, 2003
 
Cells:      Cerebral endothelial
Enzyme(s)     Collagenase Type 3: 0.2%
Medium     MEM


Brain: Rat
  • Rat, SD, E18
Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I and Barker, J: GABA(B) receptors mediate motility signals for migrating embryonic cortical cells , Cereb Cortex 11, 744-53, 2001
 
Cells:      Cortical cells
Enzyme(s)     Papain: 20 u/ml
Deoxyribonuclease I: .005%
Medium     EBSS


Brain: Rat
  • Rat, wistar, 250-300 g
Brzezinska AK, Gebremedhin D, Chilian WM, Kalyanaraman B, Elliott SJ.: Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells , Am J Physiol Heart Circ Physiol 278, H1883, 2000
 
Cells:      Cerebral artery smooth muscle cells
Enzyme(s)     Papain: 1.5 mg/ml
Collagenase Type 4: 1.5 mg/ml
Medium     Physiological Salt Solution


Brain: Rat
  • Rat, SD, E16
Varney MA, Cosford ND, Jachec C, Rao SP, Sacaan A, Lin FF, Bleicher L, Santori EM, Flor PJ, Allgeier H, Gasparini F, Kuhn R, Hess SD, Velicelebi G, Johnson EC: SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5 , J Pharmacol Exp Ther 290, 170, 1999
 
Cells:      Cortical neurons
Enzyme(s)     PDS kit: with modifications
Medium     Neurobasal Medium


Brain: Rat
  • Rat, wistar, neonatal
Boehm S, Betz H: Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors , J Neurosci 17, 4066, 1997
 
Cells:      Hippocampal cells
Enzyme(s)     Papain: 1 mg/ml
Medium     DMEM


Brain: Rat
  • Rat, SD, Fisher
Brewer, G.J.: Isolation and culture of adult rat hippocampal neurons of any age , J Neurosci Methods 71, 143, 1997
 
Cells:      Hippocampal neurons
Enzyme(s)     Papain: 0.2%
Medium     HibernateA/B27


Brain: Rat
  • Rat, E18
Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE: Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis , Proc Natl Acad Sci U S A 94, 2648-53, 1997
 
Cells:      Hippocampal and cortical neurons
Enzyme(s)     PDS kit: per instructions
Medium     Neurobasal media


Brain: Rat
  • Rat, SD, newborn
Pixley, S.K.: The Olfactory Nerve Contains Two Populations of Glia , In Vitro 5, 269, 1992
 
Cells:      Glial
Enzyme(s)     Trypsin: 0.0625%
Medium     MEM, sterile


Brain: Rat
  • Rat, newborn
Holzwarth, J., Glaum, S., and Miller, R.: Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes , Glia 5, 239, 1992
 
Cells:      Astrocytes
Enzyme(s)     Trypsin: 0.25%
Medium     DMEM, HBSS


Brain: Rat
  • Rat, 60-72 hours old
Acheson, A., Barker, P., Alderson, R., Miller, F., and Murphy, R.: Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF , Neuron 7, 265, 1991
 
Cells:      Fibroblasts
Enzyme(s)     Trypsin: 0.2%
Medium     HEPES buffered DMEM


Brain: Rat
  • Rat, SD, adult
Doron, D., Jacobowitz, D., Heldman, E., Feurerstein, G., Pollard, H., and Hallenbeck, J.: Extracellular Matrix Permits the Expression of Von Willebrand's Factor, Uptake of Di-I-Acetylated Low Density Lipoprotein and Secretion of Prostacyclin in Cultures of Endothelial Cells from Rat Brain Microvessels , In Vitro Cell Dev Biol 27A, 689, 1991
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 2: 0.5%
Medium     Medium 199


Brain: Rat
  • Rat, SD, 5 day
Mattson, M., and Kater, S.: Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions , Brain Res 490, 110, 1989
 
Cells:      Hippocampal neurons
Enzyme(s)     Trypsin: 0.2%
Deoxyribonuclease I: 10 ug/ml
Medium     HBSS


Brain: Rat
  • Rat, SD, female
Matsuda, M.: Serum Proteins Enhance Aggregate Formation of Dissociated Fetal Rat Brain Cells in an Aggregating Culture , In Vitro Cell Dev Biol 24 (10), 1031, 1988
 
Cells:      Fetal rat brain
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     DMEM


Brain: Rat
  • Rat, albino, adult and newborn
Giulian, D. and Baker, T.J.: Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain , J Neurosci 6, 2163, 1986
 
Cells:      Cerebral cortices
Enzyme(s)     Trypsin: 0.25%
Medium     PBS


Brain: Rat
  • Rat, Wistar, newborn
Goldman, J.E., Geier, S.S., and Hirano, M.: Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture , J Neurosci 6, 52, 1986
 
Cells:      Germinal matrix
Enzyme(s)     Trypsin: 0.25%
Medium     HBSS


Brain: Rat
  • Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.: An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts , J Neurosci 4, 1944, 1984
 
Cells:      Hippocampal neurons
Enzyme(s)     Trypsin: 0.25%
Medium     HBSS, CMF


Brain: Rat
  • Rat, fetus, 18 day
Rothman, S.: Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death , J Neurosci 4 (7), 1884, 1984
 
Cells:      Hippocampi
Enzyme(s)     Trypsin: 0.1%
Medium     HBSS


Brain: Rat
  • Rat, SD, 19-20 days pregnant
Ahmed, Z., Walker, P., and Fellows, R.: Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture , J Neurosci 3 (12), 2448, 1983
 
Cells:      Neural
Enzyme(s)     Trypsin: 0.25%
Medium     DMEM


Brain: Rat
  • Rat, SD, fetal
Peterfreund, R. and Vale, W.: High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell , Brain Res 239, 463, 1982
 
Cells:      Cerebral cortex
Hypothalmus
Enzyme(s)     Deoxyribonuclease I: 0.001%
Medium     HEPES


Brain: Rat
  • Rat, Wistar-Kyoto, male, 100 - 200 g
Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.: Primary Culture of Rat Cerebral Microvascular Endothelial Cells , Lab Invest 46, 554, 1982
 
Cells:      Endothelial
Cerebral
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     HBSS


Brain: Rat
  • Rat, fetus
Swaiman, K., Neale, E., Fitzgerald, S., and Nelson, P.: A Method for Large-scale Production of Mouse Brain Cortical Cultures , Brain Res 255, 361, 1982
 
Cells:      Cortical
Enzyme(s)     Trypsin: 0.25%
Medium     Puck's D1 (see reference)


Brain: Rat
  • Rat, Wistar, pregnant
Abney, E., Bartlett, P., and Raff, M.: Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain , Dev Biol 83, 301, 1981
 
Cells:      Glial
Enzyme(s)     Trypsin: 0.05%
Medium     Eagle's MEM/DMEM


Brain: Rat
  • Rat, SD, one month old
Bowman, P., Betz, A., Ar, D., Wolinsky, J., Penney, J., Shivers, R., and Goldstein, G.: Primary Culture of Capillary Endothelium From Rat Brain , In Vitro 17 (4), 353, 1981
 
Cells:      Capillary endothelium
Pericytes
Enzyme(s)     Neutral Protease: 0.1%
Medium     Medium 199


Brain: Rat
  • Rat, Wistar, male, 300-500 g
Williams, S., Gillis, J., Matthews, M., Wagner, R., and Bitensky, M.: Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity , J Neurochem 35 (2), 374, 1980
 
Cells:      Microvessels
Endothelial
Enzyme(s)     Collagenase Type 2: 0.75%
Medium     Ringers-HEPES buffer


Brain: Rat
  • Rat, Wistar, adult, 170 g
Phillips, P., Kumar, P., Kumar, S., and Waghe, M.: Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter , J Anat 129, 261, 1979
 
Cells:      Endothelial
Enzyme(s)     Trypsin: 0.5%
Medium     BSS


Brain: Rat
  • Rat, Holtzmann, 18 day
Banker, G., and Cowan, M.: Rat Hippocampal Neurons in Dispersed Cell Culture , Brain Res 126, 397, 1977
 
Cells:      Hippocampal neurons
Enzyme(s)     Trypsin: 0.1%
Medium     HBSS


Brain: Rat
  • Rat, SD, 2 days
Rowe, V., Neale, E., Avins, L., Guroff, G., and Schrier, B.: Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons , Exp Cell Res 104, 345, 1977
 
Cells:      Pineal
Enzyme(s)     Trypsin: 0.25%
Medium     DMEM, MEM


Brain: Shellfish
  • Helisoma trivolvis
Hadley, R.D., Bodnar, D.A., and Kater, S.B.: Formation of Electrical Synapses Between Isolated, Cultured Helisoma Neurons Requires Mutual Neurite Elongation , J Neurosci 5, 3145, 1985
 
Cells:      Neurons
Buccal ganglia
Enzyme(s)     Trypsin: 0.2%
Medium     Saline, Sterile


Cartilage: Bovine
  • Bovine, 18-36 month
White, R. and Gibson, J.: The Effect of Oxygen Tension on Calcium Homeostasis in Bovine Articular Chondrocytes. , J Orthop Surg Res 5, 27, 2010
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Cartilage: Bovine
  • Bovine, 6-8 week
Hwang, Y., Sangaj, N. and Varghese, S.: Interconnected Macroporous Poly(ethylene glycol) Cryogels as a Cell Scaffold for Cartilage Tissue Engineering. , Tissue Eng Part A 16, 3033-41, 2010
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM


Cartilage: Bovine
  • Bovine, 1-3 month
Lima, E., Tan, A., Tai, T., Marra, K., DeFail, A., Ateshian, G. and Hung, C.: Genipin Enhances the Mechanical Properties of Tissue-Engineered Cartilage and Protects Against Inflammatory Degradation When Used as a Medium Supplement. , J Biomed Mater Res 91, 692, 2009
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 4: 390 u/ml
Medium     DMEM


Cartilage: Bovine
  • Steers, 1-2 years
Mackintosh, D., and Mason, R.: Pharmacological Actions of 17 Beta-oestradiol on Articular Cartilage Chondrocytes and Chondrosarcoma Chondrocytes in the Absence of Oestrogen Receptors , Biochim Biophys Acta 964, 295, 1988
 
Cells:      Chondrocytes
Enzyme(s)     Trypsin: 0.20%
Medium     HBSS


Cartilage: Bovine
  • Calf, 1-14 days
Klagsbrun, M.: Large Scale Preparation of Chondrocytes ,Methods of Enzymology Vol. 58,, , 560, 1979
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.20%
Medium     PBS


Cartilage: Canine
  • Canine
Lee, J., Kim, H., Kim, J., Bae, S., Joo, D., Huh, K., Fang, Y., Jeong, J., Kim, M. and Kim, Y.: Microencapsulation of Pancreatic Islets with Canine Ear Cartilage for Immunoisolation. , Transplant Proc 44, 1091-4, 2012
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.3%
Trypsin: 0.25%
Medium     Ham's F12


Cartilage: Chicken
  • Chick embryos, HH stage
Wong, M., and Tuan, R.: Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture , In Vitro Cell Dev Biol 29A, 917, 1993
 
Cells:      Mesenchymal
Enzyme(s)     Trypsin: 0.1%
Medium     DMEM
(see reference)


Cartilage: Chicken
  • Chicken, broiler strain, 8-10 weeks
Genge, B., Wu, L. and Wuthier, R.: Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins , J Biol Chem 265, 4703, 1990
 
Cells:      Matrix vesicles
Epiphyseal growth plate
Enzyme(s)     Trypsin: 0.1%
Medium     Tris-buffered saline


Cartilage: Chicken
  • Chicken, broiler strain, 8-10 week
Genge, B.R., Wu, L.N.Y., and Wuthier, R.E.: Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles , J Biol Chem 264, 10917, 1989
 
Cells:      Matrix vesicles
Enzyme(s)     Trypsin: 0.1%
Medium     (see reference)


Cartilage: Chicken
  • Chick embryos, White Leghorn, 19 day old
Gionti, E., Capasso, O., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro , J Biol Chem 258 (11), 7190, 1983
 
Cells:      Fibroblasts
Epithelial-like
Enzyme(s)     Trypsin: 0.25%
Medium     E 199 medium


Cartilage: Chicken
  • Chick embryos, 19 day old
Capasso, O., Gionti, E., Pontarelli, G., Ambesi-Impiobato, F., Nitsch, L., Tajana, G., and Cancedda, R.: The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro , Exp Cell Res 142, 197, 1982
 
Cells:      Chondrocytes
Enzyme(s)     Trypsin: 0.75%
Medium     Coon's modified F-12


Cartilage: Chicken
  • Chick embryos, White Leghorn
Ahrens, P., Solursh, M., and Reiter, R.: Stage-Related Capacity for Limb Chondrogenesis in Cell Culture , Dev Biol 60, 69, 1977
 
Cells:      Wing buds
Enzyme(s)     Trypsin: 0.1%
Medium     Saline G


Cartilage: Equine
  • Equine
Visser, J., Levett, P., te Moller, N., Besems, J., Boere, K., van Rijen, M., de Grauw, J., Dhert, W., van Weeren, P. and Malda, J.: Crosslinkable Hydrogels Derived from Cartilage, Meniscus, and Tendon Tissue. , Tissue Eng Part A 21, 1195-206, 2015
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.15%
Medium     DMEM


Cartilage: Equine
  • Equine
Ley, C., Svala, E., Nilton, A., Lindahl, A., Eloranta, M., Ekman, S. and Skioldebrand, E.: Effects of High Mobility Group Box Protein-1, Interleukin-1B, and Interleukin-6 on Cartilage Matrix Metabolism in Three-Dimensional Equine Chondrocyte Cultures. , Conn Tissue Res 52, 290-300, 2011
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.08%
Medium     DMEM/F12


Cartilage: Goat
  • Goat
Bekkers,J, Creemers, L, Tsuchida, A, van Rijen, M, Custers, R, Dhert, W and Saris, D.: One-Stage Focal Cartilage Defect Treatment with Bone Marrow Mononuclear Cells and Chondrocytes Leads to Better Macroscopic Cartilage Regeneration Compared to Microfracture in Goats. , Osteoarthritis Cartilage 21, 950-6, 2013
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 2%
Medium     DMEM


Cartilage: Human
  • Human
Muraglia, A., Nguyen, V., Nardini, M., Mogni, M., Coviello, D., Dozin, B., Strada, P., Baldelli, I., Formica, M., Cancedda, R. and Mastrogiacomo, M.: Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support. , Front Bioeng Biotechnol 5, 66, 2017
 
Cells:      Chondrocytes
Enzyme(s)     Trypsin: 0.25%
Hyaluronidase: 0.1%
Collagenase Type 2: 1000 u/ml
Collagenase Type 1: 400 u/ml
Medium     Ham's F12


Cartilage: Human
  • Human
Andrews, S., Kunze, M., Mulet-Sierra, A., Williams, L., Ansari, K., Osswald, M. and Adesida, A.: Strategies to Mitigate Variability in Engineering Human Nasal Cartilage. , Sci Rep 7, 6490, 2017
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.15%
Medium     DMEM


Cartilage: Human
  • Human
Capsoni, F., Ongari, A., Lonati, C., Accetta, R., Gatti, S. and Catania, A.: Alpha-Melanocyte-Stimulating-Hormone (a-MSH) Modulates Human Chondrocyte Activation Induced by Proinflammatory Cytokines. , BMC Musculoskelet Disord 16, 154, 2015
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.15%
Medium     DMEM


Cartilage: Human
  • Human
Tamai, M., Nagasao, T., Yanaga, H., Hamamoto, Y., Kogure, T. and Tanaka, Y.: Correction of Secondary Deformity after Nuss Procedure for Pectus Excavatum by Means of Cultured autologous Cartilage Cell Injection. , Int J Surg Case Rep 15, 70-3, 2015
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase: 0.3%
Medium     DMEM


Cartilage: Human
  • Human, 52-82 years
Pallu, S., Francin, P., Guillaume, C., Gegout-Pottie, P., Netter, P., Mainard, D., Terlain, B. and Presle, N.: Obesity Affects the Chondrocyte Responsiveness to Leptin in Patients with Osteoarthritis. , Arthritis Res Ther 12, R112, 2010
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.2%
Pronase: 0.15%
Medium     DMEM/F12


Cartilage: Human
  • Human
Kim Wan-Uk, Kwok Seung-Ki, Hong Kyung-Hee, Yoo Seung-Ah, Kong Jin-Sun, Choe Jongseon, Cho Chul-Soo: Soluble Fas ligand inhibits angiogenesis in rheumatoid arthritis , Arthritis Res Ther 9, R42, 2007
 
Cells:      Synoviocytes
Enzyme(s)     Collagenase Type 1: 0.4%
Medium     DMEM


Cartilage: Human
  • Human, 26-68 year
Marsano A, Millward-Sadler SJ, Salter DM, Adesida A, Hardingham T, Tognana E, Kon E, Chiari-Grisar C, Nehrer S, Jakob M, Martin I: Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes , Osteoarthritis Cartilage 15, 48-58, 2007
 
Cells:      Meniscus and cartilage
Enzyme(s)     Collagenase Type 2: 0.15%
Medium     DMEM


Cartilage: Human
  • Human, 64-83 yr
Tallheden T, Bengtsson C, Brantsing C, Sjogren-Jansson E, Carlsson L, Peterson L, Brittberg M, and Lindahl A.: Proliferation and Differentiation Potential of Chondrocytes from Osteoarthritic Patients , Arthritis Res Ther 7(3), R560, 2005
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.08%
Medium     DMEM/F12


Cartilage: Human
  • Human, adult
Liagre B, Vergne-Salle P, Corbiere C, Charissoux JL, and Beneytout JL.: Diosgenin, a Plant Steroid, Induces Apoptosis in Human Rheumatoid Arthritis Synoviocytes with Cyclooxygenase-2 Overexpression , Arthritis Res Ther 6(4), R373, 2004
 
Cells:      Synovial
Enzyme(s)     Collagenase: 0.15%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.015%
Medium     DMEM


Cartilage: Human
  • Human, adult
Jakob M, Demarteau O, Schafer D, Stumm M, Heberer M, and Martin I.: Enzymatic Digestion of Adult Human Articular Cartilage Yields a Small Fraction of the Total Available Cells , Conn Tissue Res 44, 173, 2003
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.15%
Medium     DMEM


Cartilage: Human
  • Human, adults
McEvoy A., Murphy E., Ponnio T., Conneely O., Bresnihan B., FitzGerald O., and Murphy E.: Activation of Nuclear Orphan Receptor NURR1 Transcription by NF-kappa B and Cyclic Adenosine 5'-Monophosphate Response Element-binding Protein in Rheumatoid Arthritis Synovial Tissue , J Immunol 168(6), 2979, 2002
 
Cells:      Synoviocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     RPMI


Cartilage: Human
  • Human, 15-60 years
Rotter N, Bonassar LJ, Tobias G, Lebl M, Roy AK, Vacanti CA: Age dependence of cellular properties of human septal cartilage: implications for tissue engineering , Arch Otolaryngol Head Neck Surg 127, 1248-52, 2001
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM


Cartilage: Human
  • Human
Sarkissian M, Lafyatis R: Integrin engagement regulates proliferation and collagenase expression of rheumatoid synovial fibroblasts , J Immunol 162, 1772-9, 1999
 
Cells:      Synovial fibroblasts
Enzyme(s)     Collagenase: 0.4%
Medium     DMEM


Cartilage: Human
  • Human
Dunham B P, Koch R J: Basic fibroblast growth factor and insulinlike growth factor I support the growth of human septal chondrocytes in a serum-free environment , Arch Otolaryngol Head Neck Surg 124, 1325-30, 1998
 
Cells:      Septal chondrocytes
Enzyme(s)     Collagenase Type 2: 0.2%
Hyaluronidase: 0.01%
Deoxyribonuclease I: 0.015%
Medium     DMEM/F12


Cartilage: Human
  • Human w/ rheumatoid arthritis
Dayer, J., Krane, S., Russell, R., and Robinson, D.: Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells , Proc Natl Acad Sci U S A 73 (3), 945, 1976
 
Cells:      Synovial tissue
Enzyme(s)     Trypsin: 0.05%
Medium     DMEM
(see reference)


Cartilage: Human
  • Human, 13-62 years
Srivastava, V.M.L., Malemud, C.J., Hough, A.J., Bland, J.H., and Sokoloff, L.: Preliminary Experience with Cell Culture of Human Articular Chondrocytes , Arthritis Rheum 17, 165, 1974
 
Cells:      Articular chondrocytes
Enzyme(s)     Trypsin: 0.2%
Medium     BSS


Cartilage: Human
  • Human, 26-84 years
Manning, W.K., and Bonner, W.M.: Isolation and Culture of Chondrocytes From Human Adult Articular Cartilage , Arthritis Rheum 10, 235, 1967
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase:
Medium     GBSS


Cartilage: Mouse
  • Mouse, 2-5 day
Otsuru, S., Hofmann, T., Raman, P., Olson, T., Guess, A., Dominici, M. and Horwitz, E.: Genomic and Functional Comparison of Mesenchymal Stromal Cells Prepared Using Two Isolation Methods. , Cytotherapy 17, 262-70, 2015
 
Cells:      Chondrocytes
Enzyme(s)     Trypsin: 0.25%
Collagenase Type 1: 86.5 u/ml
Medium     DMEM


Cartilage: Mouse
  • Mouse, 1 day
Terpstra, L, Prud'homme, J, Arabian, A, Takeda, S, Karsenty, G, Dedhar, S, and St-Arnaud, R.: Reduced Chondrocyte Proliferation and Chondrodysplasia in Mice Lacking the Integrin-linked Kinase in Chondrocytes , J Cell Biol 162, 139, 2003
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase: 0.2%
Medium     DMEM


Cartilage: Ovine
  • Sheep, 2 month
Kojima Koji, Bonassar Lawrence J, Roy Amit K, Mizuno Hirokazu, Cortiella Joaquin, Vacanti Charles A: A Composite Tissue-Engineered Trachea Using Sheep Nasal Chondrocyte and Epithelial Cells , FASEB J 17, 823-8, 2003
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.3%
Medium     Ham's F-12


Cartilage: Porcine
  • Porcine, 1 year
Chowdhury, T., Schulz, R., Rai, S., Thuemmler, C., Wuestneck, N., Bader, A and Homandberg, G: Biomechanical Modulation of Collagen Fragment-Induced Anabolic and Catabolic Activities in Chondrocyte/Agarose Constructs. , Arthritis Res Ther 12, R82, 2010
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     DEMEM


Cartilage: Porcine
  • Porcine, 2-4 month
Graff RD, Lazarowski ER, Banes AJ, Lee GM: ATP release by mechanically loaded porcine chondrons in pellet culture , Arthritis Rheum 43, 1571-9, 2000
 
Cells:      Chondrons
Enzyme(s)     Neutral Protease: 0.3%
Collagenase: 0.2%
Medium     PBS


Cartilage: Rabbit
  • Rabbit, New Zealand, 1.2-1.4 kg
Ju, X., Deng, M., Ao, Y., Yu, C., Wang, J., Yu, J., Cui, G. and Hu, Y.: Protective Effect of Sinomenine on Cartilage Degradation and Chondrocytes Apoptosis. , Yakugaku Zasshi 130, 1053-60, 2010
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.025%
Pronase: 0.2%
Medium     DMEM


Cartilage: Rabbit
  • Rabbit, New Zealand, 1.8-2.3kg
Mehraban F, Tindal MH, Proffitt MM, Moskowitz RW.: Temporal pattern of cysteine endopeptidase (cathepsin B) expression in cartilage and synovium from rabbit knees with experimental osteoarthritis: gene expression in chondrocytes in response to interleukin-1 and matrix depletion , Ann Rheum Dis 56, 108, 1997
 
Cells:      Chondrocytes
Enzyme(s)     Hyaluronidase: .05%
Collagenase Type 2: 0.2%
Trypsin: 0.2%
Medium     Gey's solution


Cartilage: Rabbit
  • Rabbit, New Zealand, white, 4-6 wk & 22-25 wk
Plaas, A., Sandy, J., and Kimura, J.: Biosynthesis of Cartilage Proteoglycan & Link Protein Articular Chondrocytes , J Biol Chem 263, 7560, 1988
 
Cells:      Chondrocytes
Enzyme(s)     Protease XIV: 5 mg/g of tissue
Medium     Ham's F-12


Cartilage: Rabbit
  • Rabbit, white, male, 8 weeks
Benya, P.D., Padilla, S.R., and Nimni, M.E.: The Progeny of Rabbit Articular Chondrocytes Synthesize Collagen Types I and III and Type I Trimer, but Not Type II , Biochemistry 16, 865, 1977
 
Cells:      Chondrocytes
Enzyme(s)     Trypsin: 0.2%
Medium     Gey's BSS


Cartilage: Rabbit
  • Rabbit, New Zealand white or Dutch, 1 week
    (also human, newborn)
Schindler, F.H., Ose, M.A., and Solursh, M.: The Synthesis of Cartilage Collagen by Rabbit and Human Chondrocytes in Primary Cell Culture , In Vitro 12, 44, 1976
 
Cells:      Chondrocytes
Enzyme(s)     Trypsin: 0.1%
Medium     Saline G, CMF


Cartilage: Rabbit
  • Rabbit, New Zealand white, immature, 2.25 - 3.3 Kg
Green, J.R., and William, T.: Articular Cartilage Repair. Behavior of Rabbit Chondrocytes During Tissue Culture and Subsequent Allografting, Clin Orthop Relat Res , 237, 1976
 
Cells:      Articular chondrocytes
Hyaline
Enzyme(s)     Trypsin: 0.2%
Medium     Gey's BSS


Cartilage: Rabbit
  • Rabbit, New Zealand white, male, 250-350 g
Bentley, G., and Greer , R.: Homotransplantation of Isolated Epiphyseal and Articular Cartilage Chondrocytes into Joint Surfaces of Rabbits , Nature 230, 385, 1971
 
Cells:      Epiphyseal
Articular cartilage
Enzyme(s)     Trypsin: 0.25%
Medium     Eagle's basal medium


Cartilage: Rat
  • Rat
Mei, L., Shen, B., Ling, P., Liu, S., Xue, J., Liu, F., Shao, H., Chen, J., Ma, A. and Liu, X.: Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. , PLoS ONE 12, e0176107, 2017
 
Cells:      Chondrocytes
Enzyme(s)     Collagenase Type 2: 0.15%
Trypsin: 0.25%
Medium     DMEM/F12


Cartilage: Rat
  • Rat, SD, young, 100-120 g
Shimomura, Y., Yoneda, T., and Suzuki, F.: Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib , Calcif Tissue Res 19, 179, 1975
 
Cells:      Chondrocytes
Enzyme(s)     Trypsin: 0.2%
Medium     Ham's F-12 medium


Colon: Guinea-Pig
  • Guinea-pig, adult
Raghupathi, R., Duffield, M., Zelkas, L., Meedeniya, A., Brookes, S., Sia, T., Wattchow, D., Spencer, N. and Keating, D.: Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. , J Physiol 591, 5959-75, 2013
 
Cells:      Enterochromaffin
Enzyme(s)     Trypsin: 0.05%
Collagenase Type 1: 0.1%
Medium     DMEM


Colon: Guinea-Pig
  • Guinea-Pig, male, 200-250g
Kang, M., Maguma, H., Smith, T., Ross, G., Dewey, W. and Akbarali, H.: The Role of β-arrestin2 in the Mechanism of Morphine Tolerance in the Mouse and Guinea Pig Gastrointestinal Tract. , J Pharmacol Exp Ther 340, 567-76, 2012
 
Cells:      Myenteric ganglia
Enzyme(s)     Collagenase Type 4: 0.2%
Protease: 0.1%
Medium     Kreb's solution


Colon: Human
  • Human
Moser, A., Spindelboeck, W., Strohmaier, H., Enzinger, C., Gattringer, T., Fuchs, S., Fazekas, F., Gorkiewicz, G., Wurm, P., Hogenauer, C. and Khalil, M.: Mucosal Biopsy Shows Immunologic Changes of the Colon in Patients with Early MS , Neurol Neuroimmunol Neuroinflamm 4, e362, 2017
 
Cells:      Lamina propria mononuclear
Enzyme(s)     Collagenase Type 1: 0.1%
Deoxyribonuclease I: 5 u/ml
Medium     HBSS


Colon: Human
  • Human
Rollo, B., Zhang, D., Stamp, L., Menheniott, T., Stathopoulos, L., Denham, M., Dottori, M., King, S., Hutson, J. and Newgreen, D.: Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon, Cell Mol Gastroenterol Hepatol 2, 92, 2016
 
Cells:      Colon enteric neural
Enzyme(s)     Neutral Protease: 0.5%
Collagenase animal free: 0.1%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 0.01%
Medium     Ham's F-12


Colon: Human
  • Human
VanDussen, K., Marinshaw, J., Shaikh, N., Miyoshi, H., Moon, C., Tarr, P., Ciorba, M. and Stappenbeck, T.: Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. , Gut 64, 911-20, 2015
 
Cells:      Gastrointestinal epithelial
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     DMEM/F12


Colon: Human
  • Human
Ali, M., Anand, S., Tangella, K., Ramkumar, D. and Saif, T.: Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. , J Vis Exp , e52532, 2015
 
Cells:      Colon
Enzyme(s)     Trypsin: 0.1%
Collagenase: 0.1%
Medium     HBSS


Colon: Human
  • Human
Raghupathi, R., Duffield, M., Zelkas, L., Meedeniya, A., Brookes, S., Sia, T., Wattchow, D., Spencer, N. and Keating, D.: Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. , J Physiol 591, 5959-75, 2013
 
Cells:      Enterochromaffin
Enzyme(s)     Trypsin: 0.05%
Collagenase Type 1: 0.1%
Medium     DMEM


Colon: Human
  • Human
Roig, A., Eskiocak, U., Hight, S., Kim, S., Delgado, O., Souza, R., Spechler, S., Wright, W. and Shay, J.: Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. , Gastroenterol 138, 1012, 2010
 
Cells:      Colonic epithelial
Enzyme(s)     Collagenase: 150 u/ml
Neutral Protease: 0.04 mg/ml
Medium     Basal X media


Colon: Human
  • Human
Zhou, J., Belov, L., Huang, P., Shin, J., Solomon, M., Chapuis, P., Bokey, L., Chan, C., Clarke, C., Clarke, S. and Christopherson, R.: Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing. , J Immunol Methods 355, 40, 2010
 
Cells:      Colorectal cancer
Enzyme(s)     Collagenase Type 4: 1%
Deoxyribonuclease I: 0.2%
Medium     HBSS


Colon: Human
  • Human
Huang, E., Hynes, M., Zhang, T., Ginestier, C., Dontu, G., Appelman, H., Fields, J., Wicha, M. and Boman, B.: Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis. , Cancer Res 69, 3382-9, 2009
 
Cells:      Colonic epithelial
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     not listed


Colon: Human
  • Human
Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. , EMBO Mol Med 1, 338-51, 2009
 
Cells:      Colon cancer
Enzyme(s)     Collagenase Type 1: 300 u/ml
Hyaluronidase: 100 u/ml
Medium     DMEM/F12


Colon: Human
  • Human
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells , Proc Natl Acad Sci U S A 104, 10158-63, 2007
 
Cells:      Cancer stem cell
Enzyme(s)     Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
Medium     RPMI-1640


Colon: Human
  • Human
Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. , Am J Physiol Gastrointest Liver Physiol 286, G804-13, 2004
 
Cells:      Colonic epithelial
Enzyme(s)     Collagenase:
Neutral Protease: 0.3%
Deoxyribonuclease I: 0.05%
Medium     RPMI 1640


Colon: Human
  • Human
Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells , Am J Physiol/Gastro 282, G1088, 2002
 
Cells:      Colonic endothelial cells
Enzyme(s)     Collagenase Type 2: 0.25%
Medium     HBSS/5%FBS


Colon: Human
  • Human
Emenaker N, Calaf G, Cox D, Basson M and Qureshi N: Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes , J Nutr 46, 96-105, 2001
 
Cells:      Colonocytes
Enzyme(s)     Collagenase: 0.15%
Medium     DMEM/F12


Colon: Human
  • Human
Hisamatsu, T., Watanabe, M., Ogata, H., Ezaki, T., Hozawa, S., Ishii, H., Kanai, T. and Hibi, T.: Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. , Cancer Res 59, 5927-31, 1999
 
Cells:      Epithelial and mucosal lymphocytes
Enzyme(s)     Neutral Protease: 0.1%
CLSPA: 0.02%
Deoxyribonuclease I: 0.01%
Medium     RPMI 1640


Colon: Human
  • Human
Gibson, P., Rosella, O., Wilson, A., Mariadason, J., Rickard, K., Byron, K. and Barkla, D.: Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate. , Carcinogenesis 20, 539, 1999
 
Cells:      Colonic epithelial
Enzyme(s)     Neutral Protease: 1.2 u/ml
Collagenase Type 4: 50 u/ml
Medium     HBSS


Colon: Human
  • Human
Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y: High Fas ligand expression on lymphocytes in lesions of ulcerative colitis , Gut 43, 48-55, 1998
 
Cells:      Lamina propria lymphocytes
Enzyme(s)     Collagenase: 25 u/ml
Medium     HBSS


Colon: Mouse
  • Mouse
Larmonier, C., Shehab, K., Laubitz, D., Jamwal, D., Ghishan, F. and Kiela, P.: Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice. , J Biol Chem 291, 8918-30, 2016
 
Cells:      Lamina propria
Enzyme(s)     Collagenase: 100 u/ml
Deoxyribonuclease I: 0.004%
Medium     RPMI 1640


Colon: Mouse
  • Mouse
Fattahi, F., Steinbeck, J., Kriks, S., Tchieu, J., Zimmer, B., Kishinevsky, S., Zeltner, N., Mica, Y., El-Nachef, W., Zhao, H., de Stanchina, E., Gershon, M., Grikscheit, T., Chen, S. and Studer, L.: Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease. , Nature 531, 105-9, 2016
 
Cells:      Colon organoid
Enzyme(s)     Collagenase Type 1: 800 u/ml
Neutral Protease: 0.013%
Medium     DMEM


Colon: Mouse
  • Mouse, 8-10 week
Round, J. and Mazmanian, S.: Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota. , Proc Natl Acad Sci U S A 107, 12204-9, 2010
 
Cells:      Lamina propria lymphocytes
Enzyme(s)     Neutral Protease: 3 u/ml
Collagenase Type 4: 0.05%
Medium     HBSS


Colon: Mouse
  • Mouse
Atarashi, K., Nishimura, J., Shima, T., Umesaki, Y., Yamamoto, M., Onoue, M., Yagita, H., Ishii, N., Evans, R., Honda, K. and Takeda, K.: ATP Drives Lamina Propria T(H)17 Cell Differentiation. , Nature 455, 808, 2008
 
Cells:      Lamina propria
Enzyme(s)     Collagenase Type 2: 0.1%
Neutral Protease: 0.1%
Deoxyribonuclease I: 0.004%
Medium     RPMI 1640


Colon: Mouse
  • Mouse, 6-8 week
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F: Phenotypic characterization of human colorectal cancer stem cells , Proc Natl Acad Sci U S A 104, 10158-63, 2007
 
Cells:      Cancer stem cell
Enzyme(s)     Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 100 u/ml
Medium     RPMI-1640


Colon: Mouse
  • Mouse
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue , Nat Protoc 2, 2307-11, 2007
 
Cells:      Lamina propria mononuclear cells
Enzyme(s)     Collagenase: 0.05%
Deoxyribonuclease I: 0.05%
Neutral Protease: 0.3%
Medium     HBSS


Colon: Mouse
  • Mouse
Abe, K., Nguyen, K., Fine, S., Mo, J., Shen, C., Shenouda, S., Corr, M., Jung, S., Lee, J., Eckmann, L. and Raz, E.: Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. , Proc Natl Acad Sci U S A 104, 17022, 2007
 
Cells:      Dentritic
Enzyme(s)     Collagenase: 300 u/ml
Deoxyribonuclease I: 0.002%
Medium     RPMI 1640


Colon: Mouse
  • Mouse
Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.: Essential role for CD103 in the T cell-mediated regulation of experimental colitis , J Exp Med 202, 1051, 2005
 
Cells:      Lympocytes
Enzyme(s)     Collagenase/Dispase: 100 u/ml
Medium     RPMI 1640


Colon: Mouse
  • Mouse, 6-8 week
Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M: Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis , Am J Physiol/Gastro 284, G595-603, 2003
 
Cells:      Lamina propria lymphocytes
Enzyme(s)     Collagenase Type 1: 0.2%
Deoxyribonuclease I: 0.01%
Medium     HBSS


Colon: Mouse
  • Mouse, 6-8 week
Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA , J Immunol 168(1), 411, 2002
 
Cells:      Lamina propria mononuclear cells
Enzyme(s)     Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
Medium     RPMI


Colon: Rat
  • Rat, adult
Somara, S., Bashllari, D., Gilmont, R. and Bitar, K.: Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA. , Am J Physiol Gastrointest Liver Physiol 300, G1022, 2011
 
Cells:      Colon smooth muscle
Enzyme(s)     Soybean Trypsin Inhibitor: 0.01%
Collagenase Type 2: 0.1%
Medium     DMEM


Endothelial: Bovine
  • Bovine
Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina: The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin , J Vasc Res 44, 19-30, 2007
 
Cells:      Bovine umbilical cord (BUVEC)
Enzyme(s)     Collagenase: 0.1%
Medium     Dulbecco's/Ham F-12


Endothelial: Bovine
  • Bovine
Yu, M., McAndrew, R., Al-Saghir, R., Maier, K., Medhora, M., Roman, R. and Jacobs, E.: Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. , J Appl Physiol 93, 1391, 2002
 
Cells:      Pulmonary artery endothelial and smooth muscle cells
Enzyme(s)     Collagenase: 0.04-0.05%
Soybean Trypsin Inhibitor: 0.04%
Medium     RPMI-1640


Endothelial: Bovine
  • Bovine, (Bos taurus), calf
Del Vecchio, P., Siflinger-Birnboim, A., Belloni, P., Holleran, L., Lum, H., and Malik, A.: Culture and Characterization of Pulmonary Microvascular Endothelial Cells , In Vitro Cell Dev Biol 28A, 711, 1992
 
Cells:      Endothelial
Pulmonary
Enzyme(s)     Collagenase: 1000 u/ml
Collagenase Type 2: 1000 u/ml
Medium     PBS, CMF


Endothelial: Bovine
  • Calf
Vender, R.: Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension , In Vitro Cell Dev Biol 28A, 403, 1992
 
Cells:      Endothelial
Enzyme(s)     Trypsin: 0.25%
Medium     HEPES


Endothelial: Bovine
  • Bovine, adult
Machi, T., Kassell, N.F., and Scheld, W.M.: Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries , In Vitro Cell Dev Biol 26, 291, 1990
 
Cells:      Cerebral artery
Endothelial
Enzyme(s)     Collagenase: 0.2%
Medium     HBSS


Endothelial: Bovine
  • Bovine
DeNucci, G., Gryglewski, R.J., Warner, T.D., and Vane, J.R.: Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled , Proc Natl Acad Sci U S A 85, 2334, 1988
 
Cells:      Endothelial
Aortic
Enzyme(s)     Trypsin: 0.05%
Medium     Krebs Ringer solution


Endothelial: Bovine
  • Bovine
Martin, T.: Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells , Biochim Biophys Acta 962, 282, 1988
 
Cells:      Endothelial, pulmonary artery
Enzyme(s)     Collagenase: 0.1%
Medium     CMF-Dulbecco's PBS


Endothelial: Bovine
  • Bovine
Carson, M.P. and Haudenschild, C.C.: Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures , In Vitro Cell Dev Biol 22, 344, 1986
 
Cells:      Endothelial
Aorta
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     PBS


Endothelial: Bovine
  • Bovine
Kinsella, Michael G. and Wight, Thomas N.: Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration , J Cell Biol 102, 679, 1986
 
Cells:      Endothelial
Aortic
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Endothelial: Bovine
  • Bovine
Gospodarowicz, D., Massoglia, S., Cheng, J., and Fujii, D.: Effect of Fibroblast Growth Factor and Lipoproteins on the Proliferation of Endothelial Cells Derived From Bovine Adrenal Cortex, Brain Cortex, and Corpus Luteum Capillaries , J Cell Physiol 127, 121, 1986
 
Cells:      Endothelial
Enzyme(s)     Collagenase: 0.5%
Medium     DMEM/Ham's F-12


Endothelial: Bovine
  • Bovine
Goetz, I.., Warren, J., Estrada, C., Roberts, E., and Krause, D.: Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain , In Vitro Cell Dev Biol 21, 172, 1985
 
Cells:      Endothelial
Brain arteries
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     Dulbecco's PBS


Endothelial: Bovine
  • Calf
Voyta, J., Via, D., Butterfield, C., and Zetter, B.: Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein , J Cell Biol 99, 2034, 1984
 
Cells:      Endothelial
Smooth muscle
Enzyme(s)     Collagenase: 0.75%
Medium     DMEM


Endothelial: Bovine
  • Bovine
Scott, D., Murray, J., and Barnes, M.: Investigation of the Attachment of Bovine Corneal Endothelial Cells , Exp Cell Res 144, 472, 1983
 
Cells:      Endothelial
Corneal
Enzyme(s)     Trypsin: 0.2%
Medium     PBS: DMEM


Endothelial: Bovine
  • Bovine, 2-3 weeks
Lee, S., Douglas, W., Deneke, S., and Fanburg, B.: Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro , In Vitro 19, 714, 1983
 
Cells:      Endothelial
Pulmonary artery
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     RPMI 1640 w/1% Fetal Bovine Serum


Endothelial: Bovine
  • Bovine
Robinson, J. and Gospodarowicz, D.: Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells , J Cell Physiol 117, 368, 1983
 
Cells:      Endothelial, Corneal
Enzyme(s)     Trypsin: 0.05%
Medium     0.01M Phosphate buffer with 0.02% EDTA
0.9% NaCl (See Reference)


Endothelial: Bovine
  • Bovine
Olander, J., Marasa, J., Kimes, R., Johnston, G., and Feder, J.: An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells , In Vitro 18, 99, 1982
 
Cells:      Endothelial
Subclavian vein
Enzyme(s)     Collagenase Type 1: 0.10%
Medium     PBS


Endothelial: Bovine
  • Bovine
Makarski, J. S.: Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells , In Vitro 17, 450, 1981
 
Cells:      Aortic
Pulmonary artery
Enzyme(s)     Collagenase Type 2: 0.10%
Medium     PBS


Endothelial: Bovine
  • Calf, fetal, 4-9 months
Rosen, E., Mueller, S., Noveral, J., and Levine, E.: Proliferative Characteristics of Clonal Endothelial Cell Strains , J Cell Physiol 107, 123, 1981
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 1: 0.25%
Medium     PBS


Endothelial: Bovine
  • Bovine, adult
Cotta-Pereira, G., Sage, H., Bornstein, P., Ross, R., and Schwartz, S.: Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis , J Cell Physiol 102, 183, 1980
 
Cells:      Aorta
Enzyme(s)     Collagenase Type 1: 125 u/ml
Medium     Dulbecco's PBS with calcium and magnesium


Endothelial: Bovine
  • Bovine, young
Ryan, U., Mortara, M., and Whitaker, C.: Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes , Tissue Cell 12, 619, 1980
 
Cells:      Pulmonary artery
Enzyme(s)     Collagenase: 0.1%
Medium     Medium 199


Endothelial: Bovine
  • Bovine
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
 
Cells:      Foreskin
Enzyme(s)     Collagenase: 0.5%
Medium     Dulbecco's MEM w/10% calf serum


Endothelial: Bovine
  • Bovine
Eskin, S., Sybers, H., Trevino, L., Lie, J., and Chimoskey, J.: Comparison of Tissue-Cultured Bovine Endothelial Cells from Aorta and Saphenous Vein , In Vitro 14, 903, 1978
 
Cells:      Saphenous Vein
Aorta
Enzyme(s)     Collagenase: 0.01%
Medium     PBS


Endothelial: Bovine
  • Bovine
Schwartz, S.M.: Selection and Characterization of Bovine Aortic Endothelial Cells , In Vitro 14, 966, 1978
 
Cells:      Endothelial
Thoracic aorta
Saphenous veins
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     PBS


Endothelial: Bovine
  • Bovine
Ryan, U.S., Clements, E., Habliston, D., and Ryan, J.W.: Isolation And Culture of Pulmonary Artery Endothelial Cells , Tissue Cell 10, 535, 1978
 
Cells:      Pulmonary artery
Enzyme(s)     Collagenase Type 2: 0.25%
Medium     Puck's solution


Endothelial: Bovine
  • Calf
Howard, B., Macarak, E., Gunson, D., and Kefalides, N.: Characterization of the Collagen Synthesized by Endothelial Cells in Culture , Proc Natl Acad Sci U S A 73 (7), 2361, 1976
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Endothelial: Canine
  • Dog
    (also human)
Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels , Brain Res Bull 21, 785, 1988
 
Cells:      Microvessels
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     DMEM


Endothelial: Canine
  • Dog, mongrel, adult
Ford, J., Burkel, W., and Kahn, R.: Isolation of Adult Canine Venous Endothelium for Tissue Culture , In Vitro 17, 44, 1981
 
Cells:      Endothelium
Jugular vein
Enzyme(s)     Trypsin: 0.1%
Medium     Earle's PBS, CMF


Endothelial: Guinea-Pig
  • Guinea pig, female, 300-350g
Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium , Am J Physiol Heart Circ Physiol 281, H1657-66, 2001
 
Cells:      Coronary endothelial
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     see reference


Endothelial: Human
  • Human
Ganguly, A., Zhang, H., Sharma, R., Parsons, S. and Patel, K.: Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions. , J Vis Exp 66, e4032, 2012
 
Cells:      Vascular endothelial
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Endothelial: Human
  • Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells , Inv Ophthal Visual Sci 48, 614-20, 2007
 
Cells:      Corneal endothelial
Enzyme(s)     Collagenase: 0.2%
Neutral Protease: 1.0%
Medium     DMEM/F12


Endothelial: Human
  • Human
Moss, S., Bates, M., Parrino, P. and Woods, TC.: Isolation of Endothelial Cells and Vascular Smooth Muscle Cells from Internal Mammary Artery Tissue. , Ochsner J 7, 133, 2007
 
Cells:      Endothelial and vascular smooth muscle
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     HBSS


Endothelial: Human
  • Human
Davis, J., Crampton, S. and Hughes. C.: Isolation of Human Umbilical Vein Endothelial Cells (HUVEC). , J Vis Exp 3, 183, 2007
 
Cells:      HUVEC
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Endothelial: Human
  • Human
Silva AP, Kaufmann JE, Vivancos C, Fakan S, Cavadas C, Shaw P, Brunner HR, Vischer U, and Grouzmann E.: Neuropeptide Y expression, localization and cellular transducing effects in HUVEC , Biol Cell 97(6), 457, 2005
 
Cells:      HUVEC
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     RPMI 1640


Endothelial: Human
  • Human
Patel, V., Logan, A., Watkinson, J., Uz-Zaman, S., Sheppard, M., Ramsden, J. and Eggo, M.: Isolation and Characterization of Human Thyroid Endothelial Cells. , Am J Physiol Endocrinol Metab 284, E168, 2003
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Endothelial: Human
  • Human
Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation. , Am J Physiol Gastrointest Liver Physiol 285, G1277, 2003
 
Cells:      Esophageal microvascular endothelial
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     MCDB-131


Endothelial: Human
  • Human
Wang D., Lehman R., Donner D., Matli M., Warren R., and Welton M.: Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells , Am J Physiol/Gastro 282, G1088, 2002
 
Cells:      Colonic endothelial cells
Enzyme(s)     Collagenase Type 2: 0.25%
Medium     HBSS/5%FBS


Endothelial: Human
  • Human
Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM, and Kirkpatrick CJ.: Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro , Exp Mol Pathol 72, 221, 2002
 
Cells:      Pulmonary vascular endothelial cells
Enzyme(s)     Neutral Protease: 1.18 u/ml
Elastase: 10 u/ml
Medium     M199


Endothelial: Human
  • Human
Takano Manabu, Meneshian Avedis, Sheikh Emran, Yamakawa Yasuhiko, Wilkins Kirsten Bass, Hopkins Elise A, Bulkley Gregory B: Rapid upregulation of endothelial P-selectin expression via reactive oxygen species generation , Am J Physiol Heart Circ Physiol 283, H2054-61, 2002
 
Cells:      HUVEC
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     PBS


Endothelial: Human
  • Human
Goolcharran, C., Cleland, J., Keck, R., Jones, A., and Borchardt, R.: Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides , AAPS PharmSci 2 (1), 5, 2000
 
Cells:      Endothelial
Enzyme(s)     Trypsin: 2%
Medium     PBS


Endothelial: Human
  • Human
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells , Circulation 101(19), 2317, 2000
 
Cells:      HUVEC, porcine pulmonary arterial endothelial cells
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM


Endothelial: Human
  • Human
Schonbeck U, Sukhova GK, Graber P, Coulter S, Libby P: Augmented expression of cyclooxygenase-2 in human atherosclerotic lesions , Am J Pathol 155, 1281-91, 1999
 
Cells:      Vascular endothelial cells
Enzyme(s)     Collagenase: 0.1%
Medium     DMEM


Endothelial: Human
  • Human
Sanyal AJ, and Mirshahi F.: A simplified method for the isolation and culture of endothelial cells from pseudointima of transjugular intrahepatic portasystemic shunts , Lab Invest 78(11), 1469, 1998
 
Cells:      Hepatic endothelial cells
Enzyme(s)     Collagenase: 0.2%
Medium     DMEM


Endothelial: Human
  • Human
Wojta J, Gallicchio M, Zoellner H, Filonzi E, Hamilton J, McGrath K: Interleukin-4 stimulates expression of urokinase-type-plasminogen activator in cultured human foreskin microvascular endothelial cells , Blood 81, 3285-92, 1993
 
Cells:      Foreskin microvascular endothelial
Enzyme(s)     Trypsin: 0.3%
Medium     HBSS


Endothelial: Human
  • Human
Lee, K, Lawley, T, Xu, Y, and Swerlick, R.: VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells , J Invest Dermatol 98, 79, 1992
 
Cells:      Endothelial
Enzyme(s)     Trypsin: 0.3.%
Medium     HBSS
(see reference)


Endothelial: Human
  • Human
Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ: Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis , Am J Physiol 262, H1088-95, 1992
 
Cells:      Vascular endothelial
Enzyme(s)     Neutral Protease: 0.15%
Trypsin: 0.25%
Medium     M-199


Endothelial: Human
  • Human
Grant, D.S., Lelkes, P.I., Fukuda, K., Kleinman, H.K.: Intracellular Mechanisms Involved in Basement Membrane Induced Blood Vessel Differentiation In Vitro , In Vitro Cell Dev Biol 27, 327, 1991
 
Cells:      Umbilical vein
HUVEC
Enzyme(s)     Collagenase: 0.1%
Medium     Cord Buffer
(See Reference)


Endothelial: Human
  • Human
Whitehead, R., and Eeden, P.: A Method For the Prolonged Culture of Colonic Epithelial Cells, J Tiss Cul Meth 13, 103, 1991
 
Cells:      Crypt cells
Enzyme(s)     Collagenase: 125 u/ml
Medium     RMPI 1640


Endothelial: Human
  • Human
Fischer, E., Stingl, A., and Kirkpatrick, C.: Migration Assay for Endothelial Cells in Multiwells Application to Studies on the Effect of Opioids , J Immunol Methods 128, 235, 1990
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     Medium 199


Endothelial: Human
  • Human
Muller WA, Ratti CM, McDonnell SL, Cohn ZA: A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions , J Exp Med 170, 399-414, 1989
 
Cells:      Human umbilical vein endothelial cells
Enzyme(s)     Collagenase Type 2: 75 u/ml
Medium     M199


Endothelial: Human
  • Human
Gerhart, D. Z., Broderius, M. A., and Drewes, L. R.: Cutlured Human and Canine Endothelial Cells from Brain Microvessels , Brain Res Bull 21, 785, 1988
 
Cells:      Microvessels
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     DMEM


Endothelial: Human
  • Human
Kubota, Y., Kleinman, H., Martin, G., and Lawley, T.: Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures , J Cell Biol 107, 1589, 1988
 
Cells:      Endothelial/HUVEC
Foreskin & umbilical cord
Enzyme(s)     Trypsin: 0.3%
Medium     HBSS/PBS, Medium 199
(see reference)


Endothelial: Human
  • Human
Hoshi, H., Kan, M., Chen, J., and McKeehan, W.: Comparative Endocrinology-Paracrinology-Autocrinology of Human Adult Large Vessel Endothelial and Smooth Muscle Cells , In Vitro Cell Dev Biol 24 (4), 309, 1988
 
Cells:      Umbilical cord
Smooth muscle
Enzyme(s)     Collagenase: 0.1%
Medium     HEPES


Endothelial: Human
  • Human
Sharefkin, J.B., Fairchild, K.D., Albus, R.A., Cruess, D.F., and Rich, N.M.: The Cytoxic Effect of Surgical Glove Powder Particles on Adult Human Vascular Endothelial Cell Cultures: Implications for Clinical Uses of Tissue Culture Techniques , J Surg Res 41, 463, 1986
 
Cells:      Endothelial
Saphenous vein
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     PBS, CMF


Endothelial: Human
  • Human
Hoshi, H., and McKeehan, W.: Isolation, Growth Requirements, Cloning, Prostacylclin Production and Life-Span of Human Adult Endothelial Cells in Low Serum Culture Medium , In Vitro Cell Dev Biol 22 (1), 51, 1986
 
Cells:      Endothelial
Enzyme(s)     Collagenase: 0.1%
Medium     HEPES


Endothelial: Human
  • Human
Marks, R.M., Czerniecki, M., and Penny, R.: Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture , In Vitro Cell Dev Biol 21, 627, 1985
 
Cells:      Endothelial
Dermal
Enzyme(s)     Trypsin: 0.3%
Medium     PBS


Endothelial: Human
  • Human
Gordon, P., Sussman, I., and Hatcher, V.: Long-Term Culture of Human Endothelial Cells , In Vitro 19, 661, 1983
 
Cells:      Fibroblasts
Foreskin
Enzyme(s)     Hyaluronidase: 0.10%
Medium     DMEM


Endothelial: Human
  • Human
Glassberg, M., Bern, M., Coughlin, S., Haudenschild, C., Hoyer, L., and Antoniades, H.: Cultured Endothelial Cells Derived from the Human Iliac Arteries , In Vitro 18, 859, 1982
 
Cells:      Iliac arteries
Enzyme(s)     Collagenase: 0.25%
Medium     PBS w/Ca++, Mg++, & BSA
(see reference)


Endothelial: Human
  • Human, adult
Ashida, E., Johnson, A., and Lipsky, P.: Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro , J Clin Invest 67, 1490, 1981
 
Cells:      Peripheral blood mononuclear
Monocytes
T cells
Endothelial
Enzyme(s)     Collagenase: 0.25%
Medium     RPMI 1640


Endothelial: Human
  • Human
Sherer, G., Fitzharris, T., Faulk, W., and LeRoy, E.: Cultivation of Microvascular Endothelial Cells from Human Preputial Skin , In Vitro 16, 675, 1980
 
Cells:      Microvascular endothelial
Neonatal foreskins
Enzyme(s)     Neutral Protease: at 1000 u/ml
Medium     Konigsberg's modification of HBSS
(See Reference)


Endothelial: Human
  • Human (adult and child)
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
 
Cells:      Foreskin
Enzyme(s)     Collagenase: 0.5%
Medium     Dulbecco's MEM w/10% calf serum


Endothelial: Human
  • Human
Jaffe, E.A., Minick, C.R., Adelman, B., Becker, C.G., and Nachman, R.: Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells , J Exp Med 144, 209, 1976
 
Cells:      Umbilical vein
Enzyme(s)     Trypsin: 100 µg/ml
Medium     Tris-HCl,0.2 M


Endothelial: Human
  • Human
Gimbrone Jr., M.A.: Culture of Vascular Endothelium , Prog Hemost Thromb 3, 1, 1976
 
Cells:      Umbilical vein
Enzyme(s)     Collagenase Type 1: 125 u/ml
Medium     Dulbecco's PBS


Endothelial: Human
  • Human
Gimbrone, M.A., Cotran, R.S., and Folkman, J.: Human Vascular Endothelial Cells in Culture: Growth and DNA Synthesis , J Cell Biol 60, 673, 1974
 
Cells:      Umbilical cord
Enzyme(s)     Collagenase: 0.1%
Medium     Dulbecco's PBS


Endothelial: Human
  • Human
Jaffe, E., Nachman, R., Becker, C., and Minick, C.: Culture of Human Endothelial Cells Derived from Umbilical Veins. Identification by Morphologic and Immunologic Criteria , J Clin Invest 52, 2745, 1973
 
Cells:      Umbilical cord
Enzyme(s)     Collagenase: 0.2%
Medium     Cord buffer
(See Reference)


Endothelial: Human
  • Human
Lewis, L.J., Haok, J.C., Maca, R.D., and Fry, G.L.: Replication of Human Endothelial Cells in Culture , Science 181, 452, 1973
 
Cells:      Umbilical cord
Enzyme(s)     Trypsin NF 1:250: 0.125%
Medium     Saline, normal


Endothelial: Human
  • Human
Fryer, D.G., Birnbaum, G., and Luttrell, C.N.: Human Endothelium in Cell Culture , J Atheroscler Res 6, 151, 1966
 
Cells:      Umbilical cord
Enzyme(s)     Trypsin NF 1:250: 0.25%
Medium     CMF solution


Endothelial: Mouse
  • Mouse, 2 month
Sabbagh, M. and Nathans, J.: A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture , Elife , 1, 2020
 
Cells:      Endothelial, CNS
Enzyme(s)     PDS kit: with modifications
Stemxyme 1: 0.1%
Medium     EC growth medium


Endothelial: Mouse
  • Mouse, 4 week
Esteban, S., Clemente, C., Koziol, A., Gonzalo, P., Rius, C., Martinez, F., Linares, P., Chaparro, M., Urzainqui, A., Andres, V., Seiki, M., Gisbert, J and Arroyo, A.: Endothelial MT1-MMP Targeting Limits Intussusceptive Angiogenesis and Colitis via TSP1/Nitric Oxide Axis. , EMBO Mol Med 12, e10862, 2020
 
Cells:      Aortic endothelial
Enzyme(s)     Collagenase Type 1: 0.6%
Elastase: 0.25%
Medium     DMEM


Endothelial: Mouse
  • Mouse
Luo, S., Truong, A. and Makino, A.: Isolation of Mouse Coronary Endothelial Cells. , J Vis Exp , , 2016
 
Cells:      Coronary endothelial
Enzyme(s)     Collagenase Type 2: 0.1%
Neutral Protease: 0.6 u/ml
Medium     Kreb's


Endothelial: Mouse
  • Mouse
Wang, J., Sun, C., Gerdes, N., Liu, C., Liao, M., Liu, J., Shi, M., He, A., Zhou, Y., Sukhova, G., Chen, H., Cheng, X., Kuzuya, M., Murohara, T., Zhang, J., Cheng, X., Jiang, M., Shull, G. and Rogers, S.: Interleukin 18 Function in Atherosclerosis is Mediated by the Interleukin 18 Receptor and the Na-Cl Co-Transporter. , Nat Med 21, 820-6, 2015
 
Cells:      Endothelial lung
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     DMEM


Endothelial: Mouse
  • Mouse
Xiao, L., McCann, J. and Dudley, A.: Isolation and Culture Expansion of Tumor-specific Endothelial Cells. , J Vis Exp , e53072, 2015
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 2: 0.1%
Neutral Protease: 0.25 u/ml
Deoxyribonuclease I: 0.0075%
Medium     DMEM


Endothelial: Mouse
  • Mouse
Kochukov, M., Balasubramanian, A., Abramowitz, J., Birnbaumer, L. and Marrelli, S.: Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. , J Am Heart Assoc 3, , 2014
 
Cells:      Cerebral artery endothelial
Enzyme(s)     Neutral Protease: 4 u/ml
Elastase: 1 u/ml
Collagenase Type 1: 120 u/ml
Medium     DMEM


Endothelial: Mouse
  • Mouse
Kazerounian, S., Gerald, D., Huang, M., Chin, R, Udayakumar, D, Zheng, N., O'Donnell, R., Perruzzi, C., Mangiante, L., Pourat, J., Phung, T., Bravo-Nuevo, A., Shechter, S., McNamara, S. and Duhadaway, K.: RhoB Differentially Controls Akt Function in Tumor Cells and Stromal Endothelial Cells During Breast Tumorigenesis. , Cancer Res 73, 50, 2013
 
Cells:      Tumor endothelial
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     HBSS


Endothelial: Mouse
  • Mouse, 8-14 week
Imoukhuede, P. and Popel, A.: Expression of VEGF Receptors on Endothelial Cells in Mouse Skeletal Muscle. , PLoS ONE 7, e44791, 2012
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 4: 0.2%
Medium     HBSS


Endothelial: Mouse
  • Mouse
Daneman, R., Zhou, L., Agalliu, D., Cahoy, J., Kaushal, A. and Barres, B.: The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells. , PLoS ONE 5, e13741, 2010
 
Cells:      Endothelial
Enzyme(s)     Papain: 40 u/ml
Deoxyribonuclease I: 125 u/ml
Medium     DPBS


Endothelial: Mouse
  • Mouse, 7-10 week
Follenzi, A., Benten, D., Novikoff, P., Faulkner, L., Raut, S. and Gupta, S.: Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. , J Clin Invest 118, 935, 2008
 
Cells:      Liver endothelial
Enzyme(s)     Collagenase: 0.03%
Medium     DMEM


Endothelial: Mouse
  • Mouse, 4 week
Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. , Am J Physiol Cell Physiol 292, C2070, 2007
 
Cells:      Endothelial kidney
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Endothelial: Mouse
  • Mouse, embryonic or yolk sac
Braren Rickmer, Hu Huiqing, Kim YungHae, Beggs HilaryE, Reichardt LouisF, Wang Rong: Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation , J Cell Biol 172, 151-62, 2006
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 3: 200 u/ml
Deoxyribonuclease I: 0.001%
Medium     PBS


Endothelial: Mouse
  • Mouse, neonatal
Cha, S., Talavera, D., Demir, E., Nath, A. and Sierra-Honigmann, M.: A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. , Microvasc Res 70, 198, 2005
 
Cells:      Microvascular endothelial
Enzyme(s)     Neutral Protease: 0.005%
Collagenase Type 1: 4%
Medium     DMEM


Endothelial: Mouse
  • Mouse, 4 week
Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells , Mol Vis 9, 171, 2003
 
Cells:      Retinal endothelial cells
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Endothelial: Mouse
  • Mouse, male
Izawa D, Tanaka T, Saito K, Ogihara H, Usui T, Kawamoto S, Matsubara K, Okubo K, and Miyasaka M: Expression profile of active genes in mouse lymph node high endothelial cells , Int Immunol 11(12), 1989, 1999
 
Cells:      Endothelial cells from lymph node
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     PBS


Endothelial: Mouse
  • Mouse, 2-4 week
Sapatino, B., Welsh, C., Smith, C., Bebo, B., and Linticum, D.: Cloned Mouse Cerebrovascular Endothelial Cells That Maintain Their Differentiation Markers for Factor VIII, Low Density Lipoprotein, and Angiotensin-Converting Enzyme , In Vitro Cell Dev Biol 29A, 923, 1993
 
Cells:      Cerebrovascular Endothelial
Enzyme(s)     Collagenase/Dispase: 0.1%
Medium     PBS


Endothelial: Porcine
  • Porcine, 6 month
Balaoing, L., Post, A., Lin, A., Tseng, H., Moake, J. and Grande-Allen, K.: Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. , PLoS ONE 10, e0130749, 2015
 
Cells:      Valvular endothelial
Enzyme(s)     Neutral Protease: 2 u/ml
Collagenase Type 2: 60 u/ml
Medium     PBS


Endothelial: Porcine
  • Porcine
Gould, R. and Butcher, J.: Isolation of Valvular Endothelial Cells. , J Vis Exp 46, e2158, 2010
 
Cells:      Valvular endothelial
Enzyme(s)     Collagenase Type 2: 300-600 u/ml
Medium     DMEM


Endothelial: Porcine
  • Porcine
    (also bovine)
Nugent, H., and Edelman, E.: Endothelial Implants Provide Long-Term Control of Vascular Repair in a Porcine Model of Arterial Injury , J Surg Res 99, 228, 2001
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 1:
Medium     DMEM w/ 10% calf serum


Endothelial: Porcine
  • Porcine
Kwak HJ, Lee SJ, Lee YH, Ryu CH, Koh KN, Choi HY, and Koh GY.: Angiopoietin-1 inhibits irradiation- and mannitol-induced apoptosis in endothelial cells , Circulation 101(19), 2317, 2000
 
Cells:      HUVEC, porcine pulmonary arterial endothelial cells
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM


Endothelial: Porcine
  • Porcine, 6-7 month
Hill-Kapturczak N, Kapturczak MH, Block ER, Patel JM, Malinski T, Madsen KM, and Tisher CC.: Angiotensin II-stimulated nitric oxide release from porcine pulmonary endothelium is mediated by angiotensin IV , J Am Soc Nephrol 10(3), 481, 1999
 
Cells:      Porcine pulmonary endothelial
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     RPMI 1640


Endothelial: Porcine
  • Porcine
Shasby, S.: Endothelial Cells Grown On Permeable Membrane Supports, J Tiss Cul Meth 14, 247, 1992
 
Cells:      Endothelial
Enzyme(s)     Trypsin: 0.25%
Medium     Medium 199


Endothelial: Porcine
  • Porcine
Vischer, P., and Buddecke, E.: Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells , Exp Cell Res 158, 15, 1985
 
Cells:      Endothelial
Enzyme(s)     Trypsin: 0.05%
Medium     DMEM


Endothelial: Porcine
  • Porcine
Dickinson, E. and Slakey, L.: Plasma-derived Serum as a Selective Agent to Obtain Endothelial Cultures from Swine Aorta , In Vitro 18, 63, 1982
 
Cells:      Endothelial
Aortic
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     Dulbecco-Vogt MEM w/o serum


Endothelial: Porcine
  • Porcine, 30-40 kg
Merrilees, M.J., and Scott, L.: Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture , Atherosclerosis 39, 147, 1981
 
Cells:      Endothelial
Thoracic aorta
Enzyme(s)     Collagenase Type 4: 0.025%
Trypsin: 0.05%
Medium     Medium 199 and 0.01M EDTA


Endothelial: Porcine
  • Porcine, 20-30 week
Slater, D.N., and Sloan, J.M.: The Porcine Endothelial Cell in Tissue Culture , Atherosclerosis 21, 259, 1975
 
Cells:      Endothelial
Aortas
Veins
Enzyme(s)     Collagenase: 0.1%
Medium     Medium 199 w/BSS and HEPES or NaHCO3


Endothelial: Porcine
  • Porcine, 60-100 days
Coulson, W.F.: The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin , Biochim Biophys Acta 237, 378, 1971
 
Cells:      Aorta
Enzyme(s)     Trypsin: 0.1%
Medium     Phosphate buffer
(see reference)


Endothelial: Rabbit
  • Rabbit
Choi, J., Ko, M., and Kay, E.: Subcellular Localization of the Expressed 18 kDa FGF-2 Isoform in Corneal Endothelial Cells , Mol Vis 6, 222, 2000
 
Cells:      Corneal endothelial cells (CEC)
Enzyme(s)     Hyaluronidase: 0.05%
Medium     DMEM


Endothelial: Rabbit
  • Rabbit, 2-3Kg
Haley, N., Shio, H., Fowler, S.: Characterization of lipid-laden aortic cells from cholesterol-fed rabbits. I. Resolution of aortic cell populations by metrizamide density gradient centrifugation , Lab Invest 37, 287, 1977
 
Cells:      Endothelial, aortic
Enzyme(s)     Elastase: 0.2%
Medium     Hanks solution


Endothelial: Rat
  • Rat, SD, male, 250-300 g
Schwertschlag, U.S., and Whorton, A.R.: Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells , J Biol Chem 263, 13791, 1988
 
Cells:      Smooth muscle, aorta
Enzyme(s)     Soybean Trypsin Inhibitor: 0.25%
Medium     HBSS with 0.2 mM Ca++


Endothelial: Rat
  • Rat, SD, male, 350 - 450 g
Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan , Anal Biochem 161, 207, 1987
 
Cells:      Lipocytes
Kupffer
Sinusoidal endothelial
Enzyme(s)     Collagenase: 0.015%
Medium     DMEM/Ham's F-12


Endothelial: Rat
  • Rat, Wistar, male, 3 mo
Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells , J Biol Chem 258, 12221, 1983
 
Cells:      Endothelial
Kupffer
Parenchymal
Enzyme(s)     Collagenase: 0.05%
Pronase: 0.25%
Medium     HBSS


Endothelial: Rat
  • Rat, Wistar-Kyoto, male, 100 - 200 g
Diglio, C.A., Grammas, P., Filiberto Giacomelli, M.S., and Wiener, J.: Primary Culture of Rat Cerebral Microvascular Endothelial Cells , Lab Invest 46, 554, 1982
 
Cells:      Endothelial
Cerebral
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     HBSS


Endothelial: Rat
  • Rat, 300 G, and pig, 30-40 Kg
Merrilees, M.J., and Scott, L.: Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture , Atherosclerosis 39, 147, 1981
 
Cells:      Endothelial
Thoracic aorta
Enzyme(s)     Collagenase Type 4: 0.025%
Trypsin: 0.05%
Medium     Medium 199 and 0.01M EDTA


Endothelial: Rat
  • Rat
Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells , Proc Natl Acad Sci U S A 76, 5217, 1979
 
Cells:      Foreskin
Enzyme(s)     Collagenase: 0.5%
Medium     Dulbecco's MEM w/10% calf serum


Endothelial: Rat
  • Rat, Wistar, adult, 170 g
Phillips, P., Kumar, P., Kumar, S., and Waghe, M.: Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter , J Anat 129, 261, 1979
 
Cells:      Endothelial
Enzyme(s)     Trypsin: 0.5%
Medium     BSS


Epithelial: Bovine
  • Bovine, fetal
Schumann, B.L., Cody, T.E., Miller, M.L., Leikauf, G.D.: Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells , In Vitro Cell Dev Biol 24, 211, 1988
 
Cells:      Epithelial
Tracheal
Enzyme(s)     Neutral Protease: 2%
Medium     Dissociation medium, CMF


Epithelial: Canine
  • Dog
Virmani, A., Naziruddin, B., Desai, V., Lowry, J., Graves, D., and Sachdev, G.: Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion , In Vitro Cell Dev Biol 28A, 120, 1992
 
Cells:      Tracheal
Enzyme(s)     Pronase: 0.1%
Medium     DMEM


Epithelial: Chicken
  • Chick, 5 day old
Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
 
Cells:      Intestinal mesenchymal and epithelial
Enzyme(s)     Collagenase: 0.03%
Medium     DMEM


Epithelial: Fish
  • Shark (Squalus acanthias)
Karnaky, Jr., K.J., Valentich, J.D., Currie, M.G., Oehlenschlager, W.F., and Kennedy, M.P.: Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells , Am J Physiol 260, 1125, 1991
 
Cells:      Rectal gland
Enzyme(s)     Collagenase: 0.2%
Medium     Ringer's solution


Epithelial: Fish
  • Shark (Squalus acanthias)
Valentich, J: Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport, J Tiss Cul Meth 13, 149, 1991
 
Cells:      Rectal gland
Enzyme(s)     Collagenase: 0.2%
Medium     Ringer's solution


Epithelial: Fish
  • Winter flounder, 200-500 g
    (Pseudopleuronectes americanus)
Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport , Am J Physiol 251, 424, 1986
 
Cells:      Renal tubule
Enzyme(s)     Trypsin: 0.2%
Trypsin: 0.2%
Medium     CMF solution


Epithelial: Frog
  • Frog, Xenopus laevis, adult, female
Heinke, B, and Clauss, W.: Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium , J Comp Physiol [B] 169, 148, 1999
 
Cells:      Colonic epithelial
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     Kreb's


Epithelial: Guinea-Pig
  • Guinea-pig, Hartley, female, 200 g
Rutten, M.: Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells, J Tiss Cul Meth 14, 235, 1992
 
Cells:      Epithelial
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Epithelial: Guinea-Pig
  • Guinea-pig, Hartley albino,
    500-600 g
Chaminadas, G., Alkhalaf, M., Remy-Martin, J.P., Propper, A.Y., and Adessi, G.L.: Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium , J Endocrinol 123, 233, 1989
 
Cells:      Endometrial
Enzyme(s)     Collagenase: 0.25%
Medium     HBSS


Epithelial: Hamster
  • Hamster, Syrian gold, male, 100-120 g
Niles,R., Kim, K.C., Hyman, B., Christensen, T., Wasano, K., Brody, J.: Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells , In Vitro Cell Dev Biol 24, 457, 1988
 
Cells:      Tracheal
Enzyme(s)     Pronase: 0.1%
Medium     MEM with Hepes, CMF


Epithelial: Hamster
  • Hamster, Syrian golden (strain CR:RGH)
McDowell, E., et al.: Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters , Am J Pathol 129, 511, 1987
 
Cells:      Tracheal
Enzyme(s)     Trypsin: 0.1%
Medium     Ham's F-12


Epithelial: Hamster
  • Hamster (strain 1516 EHS and Lakeview), 8-12 weeks
Lee, T., Wu, R., Brody, A., Barrett, J., and Nettesheim, P.: Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture , Exp Lung Res 6, 27, 1984
 
Cells:      Tracheal
Enzyme(s)     Trypsin: 0.05%
Medium     Medium 199


Epithelial: Hamster
  • Hamster, Syrian gold, male, 6 weeks- 4 months
Goldman, W.E., Baseman, J.B.: Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea , In Vitro 16, 313, 1980
 
Cells:      Tracheal
Enzyme(s)     Trypsin: 0.25%
Medium     PBS with EDTA


Epithelial: Human
  • Human
Gottipamula, S., Saraswat, S. and Sridhar, K.: Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells. , Cytotherapy 19, 263-271, 2017
 
Cells:      Epithelial
Enzyme(s)     Collagenase Type 4: 0.1%
Neutral Protease: 0.1%
Medium     PBS


Epithelial: Human
  • Human
Hsueh, Y., Huang, S., Lai, J., Ma, S., Chen, H., Wu, S., Wang, T., Sun, C., Ma, K., Chen, J., Lai, C. and Ma, D.: Preservation of Epithelial Progenitor Cells from Collagenase-Digested Oral Mucosa During Ex Vivo Cultivation. , Sci Rep 6, 36266, 2016
 
Cells:      Oral mucosal epithelial
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     DMEM/F12


Epithelial: Human
  • Human
VanDussen, K., Marinshaw, J., Shaikh, N., Miyoshi, H., Moon, C., Tarr, P., Ciorba, M. and Stappenbeck, T.: Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. , Gut 64, 911-20, 2015
 
Cells:      Gastrointestinal epithelial
Enzyme(s)     Collagenase Type 1: 0.2%
Medium     DMEM/F12


Epithelial: Human
  • Human
Widdicombe JH, Sachs LA, Morrow JL, and Finkbeiner WE.: Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function , Biotechniques 39(2), 249, 2005
 
Cells:      Human tracheal epithelium
Enzyme(s)     Protease Type XIV: 0.04%
Medium     DMEM/F12


Epithelial: Human
  • Human
Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. , Am J Physiol Gastrointest Liver Physiol 286, G804-13, 2004
 
Cells:      Colonic epithelial
Enzyme(s)     Collagenase:
Neutral Protease: 0.3%
Deoxyribonuclease I: 0.05%
Medium     RPMI 1640


Epithelial: Human
  • Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet , Inv Ophthal Visual Sci 44(10), 4275, 2003
 
Cells:      Corneal limbal epithelial sheet
Enzyme(s)     Neutral Protease: 5%
Medium     see reference


Epithelial: Human
  • Human
Fahlgren, A., Hammarstrom, S., Danielsson, A. and Hammarstrom, M.: Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. , Clin Exp Immunol 131, 90, 2003
 
Cells:      Intestinal epithelial
Enzyme(s)     Collagenase Type 4: 72.5 u/ml
Medium     HBSS


Epithelial: Human
  • Human
Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22, 133, 2000
 
Cells:      Gastric epithelial cells
Enzyme(s)     Collagenase Type 2: 200 u/ml
Neutral Protease: 1.2 u/ml
Soybean Trypsin Inhibitor: 0.125%
Medium     L-15


Epithelial: Human
  • Human
Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R and Little, C: The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:10.1, 1995
 
Cells:      Gastric
Enzyme(s)     Collagenase Type 4: 0.01%
Medium     F-12 medium


Epithelial: Human
  • Human
Halbert CL, Alexander IE, Wolgamot GM, Miller AD: Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells , J Virol 69, 1473-9, 1995
 
Cells:      Nasal polyp epithelial
Enzyme(s)     Neutral Protease: .004%
Trypsin: 0.1%
Medium     see reference


Epithelial: Human
  • Human
Sabatini, L., Allen-Hoffmann, B, Warner, T., and Azen, E.: Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands , In Vitro Cell Dev Biol 27A, 939, 1991
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.05%
Medium     DMEM


Epithelial: Human
  • Human
Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13, 95, 1991
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.2%
Medium     MEM, PBS


Epithelial: Human
  • Human
Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples , In Vitro Cell Dev Biol 26, 1186, 1990
 
Cells:      Epithelial
Enzyme(s)     Collagenase: 2.0%
Medium     DMEM/Ham's F-12


Epithelial: Human
  • Human
Gruenert, D.C., Basbaum, C.B., and Widdicombe, J.H.: Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions , In Vitro Cell Dev Biol 26, 411, 1990
 
Cells:      Epithelial
Enzyme(s)     Pronase: 0.1%
Medium     PBS


Epithelial: Human
  • Human
Wood, L. and Neufeld, E.: A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux , J Biol Chem 265, 12796, 1990
 
Cells:      Epithelial
Sweat gland
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     (see reference)


Epithelial: Human
  • Human
    (also bovine)
Munson, L., Chandler, S., and Schlafer, D.: Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells, J Tiss Cul Meth 11 (3), 129, 1988
 
Cells:      Endometrial epithelial
Enzyme(s)     Trypsin:
Medium     DMEM/Ham's F-12


Epithelial: Human
  • Human
Widdicombe, J.H., Coleman, D.L., Finkbeiner, W.E., and Tuet, I.K.: Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa , J Appl Physiol 58, 1729, 1985
 
Cells:      Epithelial
Enzyme(s)     Deoxyribonuclease I: 0.01%
Medium     HEPES with 5.9mM Glucose,5mM DTT


Epithelial: Human
  • Human
Yankaskas, J., Cotton, C., Knowles, M., Gatzy, J., and Boucher, R.: Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports , Am Rev Respir Dis 132, 1281, 1985
 
Cells:      Epithelial
Enzyme(s)     Protease Type XIV: 0.1%
Medium     Eagle's MEM


Epithelial: Human
  • Human, women, 27-49 years
Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture , In Vitro 20, 743, 1984
 
Cells:      Epithelial
Ovary
Enzyme(s)     Trypsin: 0.125%
Medium     HBSS, CMF


Epithelial: Human
  • Human, infant and neonate
Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures , Methods Cell Biol 21, 195, 1980
 
Cells:      Epithelial
Prostate
Enzyme(s)     Trypsin: 0.1%
Medium     HBSS


Epithelial: Mouse
  • Mouse, 8-12 week
Brann, D., Tsukahara, T., Weinreb, C., Lipovsek, M., Van den Berge, K., Gong, B., Chance, R., Macaulay, I., Chou, H., Fletcher, R., Das, D., Street, K., de Bezieux, H., Choi, Y., Risso, D. and Dudoit, S.: Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. , Sci. Adv. 6, , 2020
 
Cells:      Nasal epithelium
Enzyme(s)     Papain: 20 u/ml
Deoxyribonuclease I: 0.005%
Medium     EBSS


Epithelial: Mouse
  • Mouse
Sun, F., Xiao, G. and Qu, Z.: Isolation of Murine Alveolar Type II Epithelial Cells. , Bio-protocol 7, , 2017
 
Cells:      Alveolar epithelial
Enzyme(s)     Neutral Protease: 0.1%
Deoxyribonuclease I: 0.01%
Medium     DMEM


Epithelial: Mouse
  • Mouse
Pardo-Saganta, A., Tata, P., Law, B., Saez, B., Chow, R., Prabhu, M., Gridley, T. and Rajagopal, J.: Parent Stem Cells Can Serve as Niches for Their Daughter Cells. , Nature 523, 597-601, 2015
 
Cells:      Tracheal epithelial
Enzyme(s)     Papain: 50 u/ml
Medium     See reference


Epithelial: Mouse
  • Mouse, adult
Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R., Ali, R. and Sowden, J.: Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. , Curr Protoc Stem Cell Biol Chapter 1, Unit 1H.4, 2011
 
Cells:      Cilary epithelial
Enzyme(s)     PDS kit: per instructions
Medium     EBSS


Epithelial: Mouse
  • Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. , Oxid Med Cell Longev 1, 33, 2008
 
Cells:      Renal tubular epithelial
Enzyme(s)     Collagenase: 200 u/ml
Soybean Trypsin Inhibitor: see reference
Medium     HBSS


Epithelial: Mouse
  • Mouse
Mathew, R., Degenhard,t K., Haramaty, L., Karp, C. and White, E.: Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. , Methods Enzymol 446, 77-106, 2008
 
Cells:      Epithelial
Enzyme(s)     Collagenase: 0.025%
Neutral Protease: 0.25%
Medium     DMEM


Epithelial: Mouse
  • Mouse, 6-8 week
Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA , J Immunol 168(1), 411, 2002
 
Cells:      Lamina propria mononuclear cells
Enzyme(s)     Collagenase Type 2: 0.015%
Deoxyribonuclease I: 0.01%
Medium     RPMI


Epithelial: Mouse
  • Mouse, 11 week
Mueller, S., Clark, J., Myers, P. and Korach, K.: Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. , Endocrinology 143, 2357, 2002
 
Cells:      Epithelial
Enzyme(s)     Collagenase Type 3: 25 u/ml
Hyaluronidase: 0.1%
Protease XIV: 0.05%
Deoxyribonuclease I: 0.04%
Medium     DMEM/F12


Epithelial: Mouse
  • Mouse, female
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y: Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis , Am J Pathol 155, 173-81, 1999
 
Cells:      Salivary gland epithelial
Enzyme(s)     Collagenase Type 1: 750 u/ml
Hyaluronidase: 500 u/ml
Medium     DMEM/F12


Epithelial: Mouse
  • Mouse, fetal 12-13 day
Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
 
Cells:      Intestinal mesenchymal and epithelial
Enzyme(s)     Collagenase: 0.03%
Medium     DMEM


Epithelial: Mouse
  • Mouse
Durban, E: Submandibular Salivary Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:2.1, 1995
 
Cells:      Submandibular salivary
Enzyme(s)     Collagenase Type 2 or 3: 0.16%
Medium     DMEM


Epithelial: Mouse
  • Mouse
Katayama, M., Kan, M.: Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation , In Vitro Cell Dev Biol 27, 533, 1991
 
Cells:      Esophageal
Enzyme(s)     Trypsin: 0.25%
Medium     PBS, CMF


Epithelial: Mouse
  • Mouse, mature, female, 6-8-wk-old
Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes , In Vitro Cell Dev Biol 27A, 713, 1991
 
Cells:      Uterine
Enzyme(s)     Trypsin: 0.2%
Trypsin: 0.2%
Medium     HBSS


Epithelial: Mouse
  • Mouse, female, 18 - 20 days
    (also 20 - 22 days)
Fukamachi, H., and McLachlan, J.: Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum , In Vitro Cell Dev Biol 27A, 907, 1991
 
Cells:      Uterine
Enzyme(s)     Trypsin: 0.25%
Medium     HBSS


Epithelial: Mouse
  • Mouse, (BALB/c), male, 3-5 months
Durban, E.M.: Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix , In Vitro Cell Dev Biol 26, 33, 1990
 
Cells:      Epithelial
Submandibular salivary gland
Enzyme(s)     Collagenase Type 3: 0.16% , 1:1 v/v
Medium     DMEM with 15 mM HEPES


Epithelial: Mouse
  • Mouse, female
Riser, M., Huff, B., and Medina, D.: Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells , In Vitro 19, 730, 1983
 
Cells:      Epithelial
Enzyme(s)     Pepsin: 0.1% and 0.05%
Pepsin: 0.1%
Medium     HBSS with 0.2% EDTA, CMF


Epithelial: Mouse
  • Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant
Yang, J., Flynn, D., Larson, L., and Hamamoto, S.: Growth in Primary Culture of Mouse Submandibular Epithelial Cells , In Vitro 18, 435, 1982
 
Cells:      Epithelial
Submandibular gland
Enzyme(s)     Collagenase Type 3: 0.1%
Medium     HBSS


Epithelial: Mouse
  • Mouse (BALB/cfC3H)
Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels , In Vitro 16, 502, 1980
 
Cells:      Mammary tumors
Epithelial
Enzyme(s)     Collagenase: 1.0%
Medium     HBSS


Epithelial: Mouse
  • Mouse, C3H, 6-8 weeks
Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver , In Vitro 15, 910, 1979
 
Cells:      Epithelial
Enzyme(s)     Collagenase: 0.10%
Medium     DMEM


Epithelial: Porcine
  • Porcine, 5-60 kg
Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells , Acta Opthalmol Scand 81(2), 170, 2003
 
Cells:      Retinal pigment epithelial cells
Enzyme(s)     Collagenase: 2%
Medium     DMEM


Epithelial: Porcine
  • Porcine
De Buysscher, E., Kennedy, J., and Mendicino, J.: Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis , In Vitro 20, 433, 1984
 
Cells:      Trachea
Enzyme(s)     Neutral Protease: 0.2%
Medium     HBSS


Epithelial: Rabbit
  • Rabbit, New Zealand white, adult
Vidrich, A., Racindranath, R., Farsi, K., and Targan, S.: A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures , In Vitro Cell Dev Biol 24 (3), 188, 1988
 
Cells:      Colon
Enzyme(s)     Neutral Protease: 0.3%
Medium     PBS


Epithelial: Rabbit
  • Rabbit, New Zealand white, male, 4-5lb.
Chew, C.S., Brown,M.R.: Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa , Biochim Biophys Acta 888, 116, 1986
 
Cells:      Gastric
Parietal and chief
Enzyme(s)     Collagenase Type 2: 0.08%
Medium     Sodium phosphate buffer


Epithelial: Rabbit
  • Rabbit, New Zealand white estrous, female, 4-5 months
Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) , Int J Gynecol Pathol 3, 348, 1984
 
Cells:      Mesothelial and surface epithelial
Ovaries
Enzyme(s)     Trypsin: 0.125%-0.5%
Medium     Medium 199


Epithelial: Rabbit
  • Rabbit, fetal
Logsdon, C.D., Bisbee, C.A., Rutten, M.J. and Machen, T.E.: Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel , In Vitro 18, 233, 1981
 
Cells:      Epithelial
Gastric
Enzyme(s)     Collagenase Type 3: 0.10%
Medium     HBSS


Epithelial: Rat
  • Rat
Mei, N., McDaniel, L., Dobrovolsky, V., Guo, X., Shaddock, J., Mittelstaedt, R., Azuma, M., Shelton, S., McGarrity, L., Doerge, D. and Heflich, R.: The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. , Toxicol Sci 115, 412, 2010
 
Cells:      Mammary epithelial
Enzyme(s)     Collagenase Type 3: 0.35%
Medium     HBSS


Epithelial: Rat
  • Rat, SD, adult, male, 8-10 weeks old
Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization , Biol Proced Online 3 (1), 35, 2001
 
Cells:      Seminiferous tubules
Enzyme(s)     Trypsin: 0.05%
Medium     Krebs-Ringer bicarbonate buffer
(see reference)


Epithelial: Rat
  • Rat, male
Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair , J Cell Sci 112, 243, 1999
 
Cells:      Alveolar epithelial
Enzyme(s)     Elastase: 40 u/ml
Medium     DMEM


Epithelial: Rat
  • Rat, embryonic
Shannon JM, Gebb SA, and Nielsen LD: Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture , Development 126, 1675, 1999
 
Cells:      Tracheal epithelial
Enzyme(s)     Collagenase Type 4: 0.05%
Neutral Protease:
Deoxyribonuclease I:
Medium     DMEM/F12


Epithelial: Rat
  • Rat, fetal, 14-15 day
Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995
 
Cells:      Intestinal mesenchymal and epithelial
Enzyme(s)     Collagenase: 0.03%
Medium     DMEM


Epithelial: Rat
  • Rat, 4-14 week
Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats , Inv Ophthal Visual Sci 34(1), 101, 1993
 
Cells:      Retinal pigment epithelial cells
Enzyme(s)     Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
Medium     CF Hanks with EDTA


Epithelial: Rat
  • Rat, SD, adult, 90-120 day old, 350-450 g
Klinefelter, G.: A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats, J Tiss Cul Meth 14, 195, 1992
 
Cells:      Epididymal epithelial
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     HBSS


Epithelial: Rat
  • Rat, 6 day old
Evans GS, Flint N, Somers AS, Eyden B, and Potten CS.: The development of a method for the preparation of rat intestinal epithelial cell primary cultures , J Cell Sci 101, 219, 1992
 
Cells:      Rat intestinal epithelial
Enzyme(s)     Neutral Protease: 0.01%
Collagenase: 300 u/ml
Medium     DMEM


Epithelial: Rat
  • Rat, SD, male, 150 - 250 g
Dial, E., Kao, Y., and Lichtenberger, L.: Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture , In Vitro Cell Dev Biol 27, 39, 1991
 
Cells:      Epithelial
Stomach
Enzyme(s)     Pronase: 0.15%
Medium     Medium 199


Epithelial: Rat
  • Rat, adult
    (also hamster)
Heimann, T., and Githens, S.: Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix , Pancreas 6 (5), 514, 1991
 
Cells:      Interlobular duct fragments
Enzyme(s)     Papain:
Medium     DMEM/Ham's F-12


Epithelial: Rat
  • Rat, SD, 200 g
Yassin, R., Clearfield, H., Katz, S., and Murthy, S.: Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro , Peptides 12, 63, 1991
 
Cells:      Colon
Enzyme(s)     Deoxyribonuclease I: 10 µg/ml
Medium     (see reference)


Epithelial: Rat
  • Rat, Wistar, neonatal
Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium , In Vitro Cell Dev Biol 27A, 625, 1991
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.1%
Medium     HBSS


Epithelial: Rat
  • Rodent, various (see reference)
Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13, 95, 1991
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.2%
Medium     MEM, PBS


Epithelial: Rat
  • Rat, 6-8 day
Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina , Curr Eye Res 10(11), 1081, 1991
 
Cells:      Retinal pigment epithelial
Enzyme(s)     Neutral Protease: 2%
Medium     DMEM


Epithelial: Rat
  • Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
    Rat (ACI/MNs) male, 8 weeks
Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus , In Vitro Cell Dev Biol 26, 713, 1990
 
Cells:      Epthelial
Enzyme(s)     Collagenase Type 3: 0.1%
Medium     Eagle's MEM Serum-free


Epithelial: Rat
  • Rat, Fisher 344, male, 8 wks old,
    250-300 g
Chang, L., Wu, R., and Nettesheim, P.: Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture , J Cell Sci 74, 283, 1985
 
Cells:      Tracheal epithelial
Enzyme(s)     Pronase: 0.5%
Medium     DMEM


Epithelial: Rat
  • Rat, Fischer, male, 4-6 weeks
Babcock, M., Marino, M., Gunning, W., and Stoner, G.: Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells , In Vitro 19, 403, 1983
 
Cells:      Epithelial
Esophagus
Enzyme(s)     Hyaluronidase: 0.1%
Medium     HEPES BSS


Epithelial: Rat
  • Rat, Fischer, Lewis and SD, male, 10-18 months
Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats , In Vitro 19, 576, 1983
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.05%
Medium     HBSS CMF


Epithelial: Rat
  • Rat, SD, female
Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma , In Vitro 18, 565, 1982
 
Cells:      Epithelial, cancer and tumor
Enzyme(s)     Collagenase: 0.1%
Medium     Eagles's MEM


Epithelial: Rat
  • Rat, Wistar, 12 day
Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels , Exp Cell Res 131, 363, 1981
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.05%
Medium     HBSS, CMF


Epithelial: Rat
  • Rat, Fischer, adult, 200-250 g
Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells , Exp Cell Res 89, 139, 1974
 
Cells:      Epithelial
Enzyme(s)     Hyaluronidase: 0.0075%
Medium     KCl-NaCl HEPES Buffer


Epithelial: Rat
  • Rat, Fischer, 10 day
Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver , Exp Cell Res 69, 106, 1971
 
Cells:      Epithelial-like
Enzyme(s)     Trypsin: 0.25%
Medium     PBS


Eye: Bovine
  • Bovine
Terlizzi, V., Kolibabka, M., Burgess, J., Hammes, H. and Harmsen, M.: The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2. , Stem Cells 36, 240-251, 2018
 
Cells:      Retinal endothelial
Enzyme(s)     Collagenase Type 4: 0.05%
Deoxyribonuclease I: 0.02%
Pronase: 0.02%
Medium     DMEM


Eye: Bovine
  • Bovine
Bryan, B. and D'Amore, P.: Pericyte Isolation and USe is Endothelial/Pericyte Coculture Models , Meth Enzymol 443, 315, 2008
 
Cells:      Pericyte
Enzyme(s)     Collagenase: 0.2%
Medium     DMEM


Eye: Bovine
  • Bovine
Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina , In Vitro 18 (7), 626, 1982
 
Cells:      Microvascular endothelial
Enzyme(s)     Collagenase/Dispase: 0.1%
Medium     MEM


Eye: Chicken
  • Chick, embryo, 6 day
Jacob Vanessa, Rothermel Andrae, Wolf Peter, Layer Paul G: Rhodopsin, violet and blue opsin expressions in the chick are highly dependent on tissue and serum conditions , Cell Death Differ 180, 159-68, 2005
 
Cells:      Retinal cells
Enzyme(s)     Trypsin: 0.005%
Deoxyribonuclease I: 0.005%
Medium     DMEM/F12


Eye: Chicken
  • Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H: ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent , Inv Ophthal Visual Sci 43, 3181-9, 2002
 
Cells:      Corneal epithelia
Enzyme(s)     Collagenase: 0.08%
Trypsin: 0.08%
Medium     HBSS


Eye: Chicken
  • Chick embryo
    (also rat)
Seigel, G.: The Golden Age of Retinal Cell Culture , Mol Vis 5, 4, 1999
 
Cells:      Retinal
Enzyme(s)     Trypsin: 0.6%
Medium     DMEM


Eye: Chicken
  • Chick, embryo, 10-14 day
Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina , In Vitro Cell Dev Biol 26, 1073, 1990
 
Cells:      Flat, retina
Enzyme(s)     Trypsin: 0.1%
Medium     Tyrode's solution, CMF


Eye: Fish
  • Fish, zebrafish
Diekmann, H., Kalbhen, P. and Fischer, D.: Characterization of Optic Nerve Regeneration using Transgenic Zebrafish. , Front Cell Neurosci 9, 118, 2015
 
Cells:      Retinal
Enzyme(s)     Papain: 10 u/ml
Medium     L-15


Eye: Fish
  • Goldfish
Graffe, M., Zenisek, D. and Taraska, J.: A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals. , J Gen Physiol 146, 109-17, 2015
 
Cells:      Retinal Bipolar
Enzyme(s)     Papain: 30 u/ml
Medium     HEPES


Eye: Human
  • Human
Yusoff,B, Riau, A., Yam, G., Halim, N. and Mehta, J.: Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy. ,Cells Vol. 11,, , , 2022
 
Cells:      Corneal stromal keratocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM/F-12


Eye: Human
  • Human
Xu, N., Chen, Y., Dean, K., Lu, X., Liu, X., Wang, W., Dean, D., Kaplan, H., Gao, L., Dong, F. and Liu, Y.: Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. , Stem Cells 35, 1579-1591, 2017
 
Cells:      Muller glia
Enzyme(s)     PDS kit: per instructions
Medium     DMEM


Eye: Human
  • Human
Sidney, L., Branch, M., Dua, H. and Hopkinson, A.: Effect of Culture Medium on Propagation and Phenotype of Corneal Stroma-Derived Stem Cells. , Cytotherapy 17, 1706-22, 2015
 
Cells:      Corneal stroma stem
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Eye: Human
  • Human
Du, Y., Roh, D., Funderburgh, M., Mann, M., Marra, K., Rubin, J., Li, X. and Funderburgh, J.: Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. , Mol Vis 16, 2680, 2010
 
Cells:      Corneal stromal stem
Enzyme(s)     Neutral Protease: 1.2 u/ml
Collagenase: 0.1%
Medium     DMEM


Eye: Human
  • Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G: A novel method of isolation, preservation, and expansion of human corneal endothelial cells , Inv Ophthal Visual Sci 48, 614-20, 2007
 
Cells:      Corneal endothelial
Enzyme(s)     Collagenase: 0.2%
Neutral Protease: 1.0%
Medium     DMEM/F12


Eye: Human
  • Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet , Inv Ophthal Visual Sci 44(10), 4275, 2003
 
Cells:      Corneal limbal epithelial sheet
Enzyme(s)     Neutral Protease: 5%
Medium     see reference


Eye: Human
  • Human, 5-65 years
Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture , Exp Eye Res 69, 97, 1999
 
Cells:      Retinal pigment epithelial (RPE)
Enzyme(s)     Trypsin: 0.25%
Medium     HBSS


Eye: Monkey
  • Macaques and baboon
Han ,Y., Jacoby, R. and Wu, S.: Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. , Brain Res 875, 175, 2000
 
Cells:      Retinal
Enzyme(s)     Papain: 20-40 u/ml
Deoxyribonuclease I: 400 u/ml
Medium     Ames' solution


Eye: Monkey
  • Monkey, cynomolgus, young adult
Whittum-Hudson JA, Taylor HR: Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection , Infect Immun 57, 2977, 1989
 
Cells:      Conjunctival lymphocytes
Enzyme(s)     Collagenase Type 1: 0.02%
Medium     RPMI 1640


Eye: Mouse
  • Mouse, 2-4 week
Liu, X., Tang, L. and Liu Y.: Mouse Muller Cell Isolation and Culture. , Bio-protocol 7, , 2017
 
Cells:      Muller
Enzyme(s)     PDS kit: per instructions
Medium     DMEM


Eye: Mouse
  • Mouse
Ortin-Martinez, A., Tsai, E., Nickerson, P., Bergeret, M., Lu, Y., Smiley, S., Comanita, L. and Wallace, V.: A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. , Stem Cells 35, 932-939, 2017
 
Cells:      Retinal
Enzyme(s)     Papain: 20 u/ml
Deoxyribonuclease I: 0.005%
Medium     PBS


Eye: Mouse
  • Mouse
Wohl, S., Jorstad, N., Levine, E. and Reh, T.: Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. , Nat Commun 8, 1603, 2017
 
Cells:      Retinal
Enzyme(s)     Papain: 20 u/ml
Deoxyribonuclease I: 0.005%
Medium     HBSS


Eye: Mouse
  • Mouse, C57BL/6
Blandford, S. and Baldridge, W.: The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i. , Biomed Res Int 2016, 8178162, 2016
 
Cells:      Retinal astrocytes
Enzyme(s)     Papain: 16.5 u/ml
Deoxyribonuclease I: 0.004%
Medium     HBSS


Eye: Mouse
  • Mouse, adult
Feodorova, Y., Koch, M., Bultman, S., Michalakis, S. and Solovei, I.: Quick and Reliable Method for Retina Dissociation and Separation of Rod Photoreceptor Perikarya from Adult Mice. , MethodsX 2, 39, 2015
 
Cells:      Retinal photoreceptor
Enzyme(s)     PDS kit: with modifications
Medium     EBSS


Eye: Mouse
  • Mouse, 4-8 day
Balmer, J., Zulliger, R., Roberti, S. and Enzmann, V.: Retinal Cell Death Caused by Sodium Iodate Involves Multiple Caspase-Dependent and Caspase-Independent Cell-Death Pathways. , Int J Mol Sci 16, 15086-103, 2015
 
Cells:      Photoreceptors
Enzyme(s)     PDS kit: per instructions
Medium     Neurobasal


Eye: Mouse
  • Mouse, embryonic and P0
Dvoriantchikova, G., Perea-Martinez, I., Pappas, S., Barry, A., Danek, D., Dvoriantchikova, X., Pelaez, D. and Ivanov, D.: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina. , PLoS ONE 10, e0131054, 2015
 
Cells:      Retinal
Enzyme(s)     Papain: 8 u/ml
Deoxyribonuclease I: 124 u/ml
Medium     DPBS


Eye: Mouse
  • Mouse
Iqbal, N., Xu, L., Devitt, C. and Skapek, S.: Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development. , Biotechniques 56, 239-49, 2014
 
Cells:      Vitreous cells
Enzyme(s)     Collagenase: 0.1%
Hyaluronidase: 0.03%
Medium     DMEM


Eye: Mouse
  • Mouse, 6 week
Singh, M., Issa, P, Butler, R., Martin, C., Lipinski, D., Sekaran, S., Barnard, A. and Maclaren, R.: Reversal of End-Stage Retinal Degeneration and Restoration of Visual Bunction by Photoreceptor Transplantation. , Proc Natl Acad Sci U S A , , 2013
 
Cells:      Retinal
Enzyme(s)     PDS kit: per instructions
Medium     DMEM


Eye: Mouse
  • Mouse
Schmidt, T. and Kofuji, P.: An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells. , J Vis Exp 47, 2367, 2011
 
Cells:      Retinal ganglion
Enzyme(s)     CLSPA: 240 u/ml
Hyaluronidase: 0.2%
Medium     Ames


Eye: Mouse
  • Mouse, adult
Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R., Ali, R. and Sowden, J.: Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. , Curr Protoc Stem Cell Biol Chapter 1, Unit 1H.4, 2011
 
Cells:      Cilary epithelial
Enzyme(s)     PDS kit: per instructions
Medium     EBSS


Eye: Mouse
  • Mouse, 4-5 week
Ebihara, L., Tong, J., Vertel, B., White, T. and Chen, T.: Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells. , Inv Ophthal Visual Sci 52, 882-9, 2011
 
Cells:      Lens Fiber
Enzyme(s)     Collagenase Type 4: 0.125%
Protease: 0.5%
Medium     PBS


Eye: Mouse
  • Mouse, postnatal day 1
Jiang, C. Klassen, H., Zhang, X. and Young, M.: Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina. , Mol Vis 16, 983, 2010
 
Cells:      Retinal progenitor
Enzyme(s)     Collagenase: 0.1%
Medium     HBSS


Eye: Mouse
  • Mouse, 4-8 week
Kobayashi, T., Yoshioka, R., Shiraishi, A. and Ohashi, Y.: New Technique for Culturing Corneal Epithelial Cells of Normal Mice. , Mol Vis 15, 1589-93, 2009
 
Cells:      Corneal epithelial
Enzyme(s)     Neutral Protease: 1.5%
Trypsin: 0.25%
Medium     DMEM/F12


Eye: Mouse
  • Mouse
Amadi-Obi, A., Yu, C., Liu, X., Mahdi, R., Clarke, G., Nussenblatt, R, Gery, I., Lee, Y. and Egwuagu, C.: TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1. , Nat Med 13, 711, 2007
 
Cells:      Retinal
Enzyme(s)     Collagenase: 0.1%
Deoxyribonuclease I: 0.001%
Medium     RPMI


Eye: Mouse
  • mouse, adult
Jadhav AP, Cho SH and Cepko CL: Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property , Proc Natl Acad Sci U S A 103, 18998, 2006
 
Cells:      Retinal
Enzyme(s)     Papain: 50 u/ml
Medium     DMEM/F-12


Eye: Mouse
  • Mouse, adult
Yoshida, S., Shimmura, S., Nagoshi, N., Fukuda, K., Matsuzaki, Y., Okano, H. and Tsubota, K.: Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. , Stem Cells 24, 2714-22, 2006
 
Cells:      Stromal
Enzyme(s)     Trypsin: 0.05%
Collagenase: 78 u/ml
Hyaluronidase: 38 u/ml
Medium     DMEM/F-12


Eye: Mouse
  • Mouse, 2-5 month
Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto: Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission , J Neurosci 25, 566-76, 2005
 
Cells:      Mouse retinal and bipolar
Enzyme(s)     Papain: 20 u/ml
Deoxyribonuclease I: 200 u/ml
Medium     HBSS


Eye: Mouse
  • Mouse, 4 week
Su X, Sorenson CM, and Sheibani N.: Isolation and characterization of murine retinal endothelial cells , Mol Vis 9, 171, 2003
 
Cells:      Retinal endothelial cells
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Eye: Porcine
  • Porcine, adult
Xu, N., Chen, Y., Dean, K., Lu, X., Liu, X., Wang, W., Dean, D., Kaplan, H., Gao, L., Dong, F. and Liu, Y.: Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. , Stem Cells 35, 1579-1591, 2017
 
Cells:      Muller glia
Enzyme(s)     PDS kit: per instructions
Medium     DMEM


Eye: Porcine
  • Porcine, 5-60 kg
Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells , Acta Opthalmol Scand 81(2), 170, 2003
 
Cells:      Retinal pigment epithelial cells
Enzyme(s)     Collagenase: 2%
Medium     DMEM


Eye: Rabbit
  • Rabbit, New Zealand, Adult
Lai, J., Cheng, H. and Ma, D.: Investigation of Overrun-Processed Porous Hyaluronic Acid Carriers in Corneal Endothelial Tissue Engineering , PLoS ONE 10, 1371, 2015
 
Cells:      Corneal endothelial
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     HBSS


Eye: Rabbit
  • Rabbit
Hao, M., Flynn, K., Nien-Shy, C., Jester, B., Winkler, M., Brown, D., La Schiazza, O., Bille, J., Jester, J.: In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelbert Two-Photon Laser Opthalmoscope , Exp Eye Res 91, 308, 2010
 
Cells:      Corneal keratocytes
Enzyme(s)     Hyaluronidase: 0.05%
Collagenase: 0.2%
Medium     DMEM


Eye: Rabbit
  • Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C: Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes , Inv Ophthal Visual Sci 45, 807-12, 2004
 
Cells:      Corneal keratocytes
Enzyme(s)     Trypsin: 0.25%
Collagenase: 0.5%
Medium     PBS


Eye: Rabbit
  • Rabbit, adult
Brockway LM, Zhou Z-H, Bubien JK, Jovov B, Benos DJ, Keyser KT: Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits , Am J Physiol Cell Physiol 283, C126-34, 2002
 
Cells:      Retinal neurons
Enzyme(s)     Papain: 26 u/ml
Medium     DMEM


Eye: Rabbit
  • Rabbit, New Zealand, adult, male, albino,
    2 kg
Johnson-Muller, B., and Gross, J.: Regulation of Corneal Collagenase Production: Epithelial-Stromal Cell Interactions , Proc Natl Acad Sci U S A 75 (9), 4417, 1978
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.25%
Medium     HBSS/DMEM


Eye: Rat
  • Adult, female, 170-200 g
Mead, B. and Tomarev, S.: Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms. , Stem Cells Transl Med 6, 1273-1285, 2017
 
Cells:      Retinal
Enzyme(s)     PDS kit: with modifications
Medium     EBSS


Eye: Rat
  • Rat, Wister, 3-30 wk
Cameron, M., Kekesi, O., Morley, J., Tapson, J., Breen, P., Van Schaik, A and Buskila, Y.: Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. , PLoS ONE 11, e0155468, 2016
 
Cells:      Retinal
Enzyme(s)     Deoxyribonuclease I: 0.005%
Papain: 30 u/ml
Medium     EBSS


Eye: Rat
  • Rat, SD, 6-8 week
Vigneswara, V., Esmaeili, M., Deer, L., Berry, M., Logan, A. and Ahmed, Z.: Eye Drop Delivery of Pigment Epithelium-Derived Factor-34 Promotes Retinal Ganglion Cell Neuroprotection and Axon Regeneration. , Mol Cell Neurosci 68, 212-21, 2015
 
Cells:      Retinal
Enzyme(s)     PDS kit: per instructions
Medium     EBSS


Eye: Rat
  • Rat, SD
Tan, C., Andrews, M., Kwok, J., Heintz, T., Gumy, L., Fassler, R. and Fawcett, J.: Kindlin-1 Enhances Axon Growth on Inhibitory Chondroitin Sulfate Proteoglycans and Promotes Sensory Axon Regeneration. , J Neurosci 32, 7325, 2012
 
Cells:      Retinal ganglion
Enzyme(s)     PDS kit: with modifications
Medium     Neurobasal


Eye: Rat
  • Rat, SD, 180-220g
Ma, J., Yu, W., Wang, Y., Cao, G., Cai, S., Chen, X., Yan, N., Yuan, Y., Zeng, H., Fleenor, D., Liu, X. and Pang, I.: Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells. , Inv Ophthal Visual Sci 51, 3544-53, 2010
 
Cells:      Retinal ganglion
Enzyme(s)     Papain: 0.2%
Medium     Neurobasal


Eye: Rat
  • Rat, Fisher, adult
Suzuki, T., Mandai, M., Akimoto, M., Yoshimura, N. and Takahashi, M.: The Simultaneous Treatment of MMP-2 Stimulants in Retinal Transplantation Enhances Grafted Cell Migration into the Host Retina. , Stem Cells 24, 2406, 2006
 
Cells:      Retinal
Enzyme(s)     PDS kit: per instructions
Medium     MEM


Eye: Rat
  • Rat, Dark Agouti, 3-4 week
Akagi T, Mandai M, Ooto S, Hirami Y, Osakada F, Kageyama R, Yoshimura N, Takahashi M.: Otx2 homeobox gene induces photoreceptor-specific phenotypes in cells derived from adult iris and ciliary tissue , Inv Ophthal Visual Sci 45, 4570, 2004
 
Cells:      Ciliary-derived eye
Enzyme(s)     PDS kit: with modifications
Medium     DMEM/F12


Eye: Rat
  • Rat, embryonic 17 day
Seigel, G.: The Golden Age of Retinal Cell Culture , Mol Vis 5, 4, 1999
 
Cells:      Retinal
Enzyme(s)     Trypsin: 0.6%
Medium     DMEM


Eye: Rat
  • Rat, SD
Jing, S., Wen, D., Yu, Y., Holst, P., Luo, Y., Fang, M., Tamir, R., Antonio, L., Hu, Z., et al.: GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF , Cell 85, 1113, 1996
 
Cells:      Retina
Enzyme(s)     Papain: 120 U
Medium     HBSS, PBS


Eye: Rat
  • Rat, 4-14 week
Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats , Inv Ophthal Visual Sci 34(1), 101, 1993
 
Cells:      Retinal pigment epithelial cells
Enzyme(s)     Collagenase Type 1: 65 u/ml
Hyaluronidase: 220 u/ml
Medium     CF Hanks with EDTA


Eye: Rat
  • Rat, 6-8 day
Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina , Curr Eye Res 10(11), 1081, 1991
 
Cells:      Retinal pigment epithelial
Enzyme(s)     Neutral Protease: 2%
Medium     DMEM


Eye: Rat
  • Rat, Long Evans, 1-15 days
Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats , J Neurosci 6, 3044, 1986
 
Cells:      Neurons, visual cortex
Enzyme(s)     Papain: 20 u/ml
Medium     BSS
(see reference)


Eye: Rat
  • Rat, SD, pups
Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat , J Neurosci 3 (12), 2532, 1983
 
Cells:      Retina
Enzyme(s)     Trypsin: 0.25%
Medium     Ham's F-12


Eye: Salamander
  • Salamander
Clarke, R, Wang, J. and Townes-Anderson, E.: Using Laser Tweezers For Manipulating Isolated Neurons In Vitro. , J Vis Exp 19, 911, 2008
 
Cells:      Neurons
Enzyme(s)     Papain: 14 u/ml
Medium     Ringers


Eye: Salamander
  • Salamander, 18-25 cm
Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase , J Cell Biol 100, 175, 1985
 
Cells:      Retina
Enzyme(s)     Papain: 14 u/ml
Medium     Saline


Eye: Salamander
  • Salamander (A. tigrinum)
Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina , Proc Natl Acad Sci U S A 75, 3507, 1978
 
Cells:      Photoreceptors, retina
Enzyme(s)     Papain: 0.05%
Medium     (see reference)


Eye: Turtle
  • Turtle (Pseudemys scripta elegans)
Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors , Proc Natl Acad Sci U S A 69, 1987, 1972
 
Cells:      Retinal
Enzyme(s)     Papain: 0.1% (13.5 u/mg)
Medium     Kreb's Ringer


Heart: Bovine
  • Bovine
    (also rat)
Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26, 335, 1990
 
Cells:      Heart
Adrenal chromaffin
Paraneurons
Enzyme(s)     Trypsin: 0.06%
Medium     25mM HEPES buffered Locke's solution, CMF


Heart: Canine
  • Canine
Zhang,D., Wu, C., Qi, X., Meijering, R., Hoogstra-Berends, F., Tadevosyan, A., Cubukcuoglu, et al: Activation of Histone Deacetylase-6 Induces Contractile Dysfunction Through Derailment of alpha-tubulin Proteostasis in Experimental and Human Atrial Fibrillation. , Circulation 129, 346-58, 2014
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 150 u/ml
Medium     Tyrode's solution


Heart: Canine
  • Canine, adult
Bonilla, I., Sridhar, A., Nishijima, Y., Gyorke, S., Cardounel, A. and Carnes, C.: Differential Effects of the Peroxynitrite Donor, SIN-1, on Atrial and Ventricular Myocyte Electrophysiology. , J Cardiovasc Pharmacol 61, 401-7, 2013
 
Cells:      Atrial and ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.065%
Medium     See reference


Heart: Canine
  • Canine
Xi, Y., Wu, G., Ai, T., Cheng, N., Kalisnik, J., Sun, J., Abbasi, S., Yang, D., Fan, C., Yuan, X., Wang, S. et al: Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium. , Circ Arrhythm Electrophysiol 6, 976-83, 2013
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 300 u/ml
Protease: 0.03%
Medium     Tyrode's solution


Heart: Canine
  • Canine
Gan, T., Qiao, W., Xu, G., Zhou, X., Tang, B., Song, J., Li, Y., Zhang, J., Li, F., Mao, T. and Jiang, T.: Aging-Associated Changes in L-type Calcium Channels in the Left Atria of Dogs. , Exp Ther Med 6, 919-924, 2013
 
Cells:      Atrial myocytes
Enzyme(s)     Collagenase Type 2: 0.03%
Medium     Tyrode's solution


Heart: Canine
  • Canine
Harleton, E., Besana, A., Comas, G., Danilo, P., Rosen, T., Argenziano, M., Rosen, M., Robinson, R. and Feinmark, S.: Ability to Induce Atrial Fibrillation in the Peri-Operative Period is Associated with Phosphorylation-Dependent Inhibition of TWIK Protein-Related Acid-Sensitive Potassium Channel 1 (TASK-1). , J Biol Chem 288, 2829-38, 2013
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 60 u/ml
Protease XIV: 0.5 u/ml
Medium     See reference


Heart: Canine
  • Canine
Zhang, H., Silva, J., Lin, Y., Verbsky, J., Lee, U., Kanter, E., Yamada, K., Schuessler, R. and Nichols, C.: Heterogeneity and Function of K(ATP) Channels in Canine Hearts. , Heart Rhythm 10, 1576-83, 2013
 
Cells:      Atrial and ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.05-0.08%
Medium     See reference


Heart: Canine
  • Canine, adult
Calloe, K., Nof, E., Jespersen, T., Di Diego, J., Chlus, N., Olesen, S.¸ Antzelevitch, C. and Cordeiro, J.: Comparison of the Effects of a Transient Outward Potassium Channel Activator on Currents Recorded from Atrial and Ventricular Cardiomyocytes. , J Cardiovasc Electrophysiol 22, 1057-66, 2011
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Protease Type XIV: 0.01%
Medium     HEPES


Heart: Canine
  • Canine, 20-25 kg
Burashnikov, A., Zygmunt, A., Di Diego, J., Linhardt, G., Carlsson, L. and Antzelevitch, C.: AZD1305 Exerts Atrial Predominant Electrophysiological Actions and is Effective in Suppressing Atrial Fibrillation and Preventing its Reinduction in the Dog. , J Cardiovasc Pharmacol 56, 80-90, 2010
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     Tyrode's solution


Heart: Canine
  • Canine, adult
Gavi, S., Yin, D., Shumay, E., Wang, H. and Malbon, C.: Insulin-Like Growth Factor-I Provokes Functional Antagonism and Internalization of Beta1-Adrenergic Receptors. , Endocrinology 148, 2653-62, 2007
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Protease: 0.008%
Medium     M199


Heart: Canine
  • Canine
Schotten, U., de Haan, S., Verheule, S., Harks, E., Frechen, D., Bodewig, E., Greiser, M., Ram, R., Maessen, J., Kelm, M., Allessie, M. and Van Wagoner, D.: Blockade of Atrial-Specific K+-currents Increases Atrial but not Ventricular Contractility by Enhancing Reverse Mode Na+/Ca2+-Exchange. , Cardiovasc Res 73, 37-47, 2007
 
Cells:      Atrial and ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.065%
Medium     Tyrode's solution


Heart: Canine
  • Canine
Baba, S., Dun, W., Hirose, M. and Boyden, P.: Sodium Current Function in Adult and Aged Canine Atrial Cells. , Am J Physiol Heart Circ Physiol 291, H756-61, 2006
 
Cells:      Atrial myocytes
Enzyme(s)     Collagenase Type 2: 0.013%
Medium     HEPES


Heart: Canine
  • Canine, 20-30 kg
Sun, H., Chartier, D., Leblanc, N. and Nattel, S.: Intracellular Calcium Changes and Tachycardia-Induced Contractile Dysfunction in Canine Atrial Myocytes. , Cardiovasc Res 49, 751-61, 2001
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 110 u/ml
Medium     Tyrode's solution


Heart: Canine
  • Canine
Gintant, G.: Two Components of Delayed Rectifier Current in Canine Atrium and Ventricle. Does IKs Play a Role in the Reverse Rate Dependence of Class III Agents? , Circ Res 78, 26-37, 1996
 
Cells:      Ventricular and atrial myocytes
Enzyme(s)     Collagenase Type 4: 0.04%
Collagenase Type 2: 125 u/ml
Medium     HEPES/Tyrode's


Heart: Canine
  • Dog, mongrel, adult
Spanier, A. and Weglicki, W.: Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis , Am J Physiol 243, H448, 1982
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.1%
Medium     Joklik's MEM


Heart: Chicken
  • Chicken, embryonic
Blech-Hermoni, Y. and Ladd, A.: Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq. , Genom Data 6, 74-76, 2015
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.13%
Collagenase Type 2: 0.13%
Deoxyribonuclease I: 0.033%
Medium     HBSS


Heart: Chicken
  • Chicken embryos, 9-11 day incubated
Eschenhagen, T., Fink, C., Remmers, U., Scholz, H., Wattchow, J., Weil, J., et al.: Three-Dimensional Reconstitution of Embryonic Cardiomyocytes in a Collagen Matrix: A New Heart Muscle Model System , FASEB J 11, 683, 1997
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.25%
Medium     PBS


Heart: Chicken
  • Chick, embryos, 10-11 day
Wang, S., Greaser, M.L., Schultz, E., Bulinski, J.C., Lin, J.J., and Lessard, J.L.: Studies on Cardiac Myofibrillogenesis With Antibodies to Titin, Actin, Tropomyosin, and Myosin , J Cell Biol 107, 1075, 1988
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.17%
Medium     HBSS, CMF


Heart: Chicken
  • Chick, 10 day
Kim, D., Okada, A., and Smith, T.W.: Control of Cytosolic Calcium Activity During Low Sodium Exposure in Cultured Chick Heart Cells , Circ Res 61, 29, 1987
 
Cells:      Ventricular
Enzyme(s)     Trypsin: 0.025%
Medium     HBSS, CMF


Heart: Chicken
  • Chick, embryo, 11 day
Jacob, R., Lieberman, M., Murphy, E., and Piwnica-Worms, D.: Effect of Sodium-Potassium Pump Inhibition and Low Sodium on Membrane Potential in Cultured Embryonic Chick Heart Cells , J Physiol 387, 549, 1987
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.05%
Medium     HBSS, CMF


Heart: Chicken
  • Chick, embryonic, 11-12-day-old
Murphy, E., Aiton, J., Russell, C., and Lieberman, M.: Calcium Elevation in Cultured Heart Cells: Its Role in Cell Injury , Am J Physiol Cell Physiol 245 (14), C316, 1983
 
Cells:      Myocytes
Enzyme(s)     Trypsin: 0.025%
Medium     CMF solution


Heart: Chicken
  • Chick, 8 day embryo
    (also rat, neonate and mouse)
Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells , Exp Cell Res 53, 1, 1968
 
Cells:      Myocytes
Enzyme(s)     Trypsin NF 1:250: 0.25%
Medium     Rinaldini's buffer solution, CF


Heart: Chicken
  • Hen, White Leghorn
Dehann, R.: Regulation of Spontaneous Activity and Growth of Embryonic Chick Heart Cells in Tissue Culture , Dev Biol 16, 216, 1967
 
Cells:      Heart
Enzyme(s)     Trypsin: 0.025%
Medium     Medium 199


Heart: Chicken
  • Chick embryos, 5 day
Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation , Exp Cell Res 30, 257, 1963
 
Cells:      Heart
Liver
Enzyme(s)     Trypsin: 3.0%
Medium     Tyrode's solution, CMF


Heart: Feline
  • Cat, mongrel, adult, 1.8-2.8 Kg
Silver, L., Hemwall, E., Marino, T., and Houser, S.: Isolation and Morphology of Calcium-Tolerant Feline Ventricular Myocytes , Am J Physiol 245, H891, 1983
 
Cells:      Myocytes
Ventricular
Enzyme(s)     Collagenase Type 2: 0.12%
Medium     Kreb's Henseleit, CF


Heart: Fish
  • Zebrafish
Fowler, E., Wang, N., Hezzell, M., Chanoit, G., Hancox, J. and Cannell, M.: Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration , Proc Natl Acad Sci U S A 117, 2687-2692, 2020
 
Cells:      Cardiac Myocytes
Enzyme(s)     Protease XIV: 0.005%
Collagenase Type 1: 0.1-0.2%
Trypsin: 0.08%
Medium     HEPES


Heart: Fish
  • Fish, Danio rerio
Sander, V., Sune, G., Jopling, C., Morera, C. and Belmonte J.: Isolation and In Vitro Culture of Primary Cardiomyocytes from Adult Zebrafish Hearts. , Nat Protoc 8, 800-9, 2013
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.5%
Collagenase Type 4: 0.5%
Medium     MEM


Heart: Frog
  • Frog (Rana esculenta)
Arrio-Dupont, M., and de Nay, D.: High Yield Preparation of Calcium-Tolerant Myocytes From Frog Ventricles, Biol Cell 54, 164, 1985
 
Cells:      Myocytes
Enzyme(s)     Trypsin: 0.04%
Medium     CF Ringer


Heart: Guinea-Pig
  • Guinea pig, male, 350-400g
Wang, Y., Geng, J., Jiang, M., Li, C., Han, Y and Jiang, J.: The Cardiac Electrophysiology Effects of Higenamine in Guinea Pig Heart , Biomed Pharmacother 109, 2348, 2019
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.4%
Medium     Tyrodes solution


Heart: Guinea-Pig
  • Guinea pig, adult
Zorn-Pauly, K., Schaffer, P., Pelzmann, B., Bernhart, E., Lang, P., and Koidl, B.: L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes , Physiol Res 53(4), 369, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 100 u/ml
Medium     M-199


Heart: Guinea-Pig
  • Guinea-Pig
Dhamoon, A, Pandit, S., Sarmast, F., Parisian, K., Guha, P., Li, Y., Bagwe, S., Taffet, S. and Anumonwo, J.: Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. , Circ Res 94, 1332-9, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 100-200 u/ml
Medium     See reference


Heart: Guinea-Pig
  • Guinea pig, female, 300-350g
Buxton I L, Kaiser R A, Oxhorn B C, Cheek D J: Evidence supporting the Nucleotide Axis Hypothesis: ATP release and metabolism by coronary endothelium , Am J Physiol Heart Circ Physiol 281, H1657-66, 2001
 
Cells:      Coronary endothelial
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     see reference


Heart: Guinea-Pig
  • Guinea pig, 250-450 g
Preisig-Muller, R., Mederos Y Schnitzler, M., Derst, C., and Daut J.: Separation of Cardiomyocytes and Coronary Endothelial Cells for Cell-Specific RT-PCR , Am J Physiol 277, H413, 1999
 
Cells:      Endothelial
Enzyme(s)     Collagenase Type 2: 0.15%
Medium     Perfusing solution
(see reference)


Heart: Guinea-Pig
  • Guinea-pig, male, 450-600 g
Stemmer, P., Akera,T., Brody, T., Rardon, D., and Watanabe, A.: Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart , Life Sci 44, 1231, 1989
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.02%
Medium     Krebs Henseleit bicarbonate buffer


Heart: Guinea-Pig
  • Guinea-pig
Ishihara, K., Mitsuiye, T., Noma, A., and Takano, M.: The Mg2+ Block and Intrinsic Gating Underlying Inward Rectification of the K+ Current in Guinea-Pig Cardiac Myocytes , J Physiol 419, 297, 1989
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase: 0.04%
Medium     Tyrode solution, CF


Heart: Guinea-Pig
  • Guinea-pig
Bridge, J., Spitzer, K., and Ershler, P.: Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent , Science 241, 823, 1988
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.10%
Medium     Bicarbonate buffer, CF


Heart: Human
  • Human
Holt-Casper, D., Theisen, J., Moreno, A., Warren, M., Silva, F., Grainger, D., Bull, D. and Patel, A.: Novel Xeno-free Human Heart Matrix-Derived Three-Dimensional Scaffolds. , J Transl Med 13, 194, 2015
 
Cells:      Cardiac
Enzyme(s)     Collagenase: 0.045%
Pancreatin: 0.1%
Medium     DPBS


Heart: Human
  • Human
Sharma, S., Mishra, R., Simpson, D., Wehman, B., Colletti, E., Deshmukh, S., Datla, S., Balachandran, K., Guo, Y., Chen, L., Siddiqui, O., Kaushal, S. and Kaushal, S.: Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome. , Stem Cells 33, 1213-29, 2015
 
Cells:      Cardiospheres
Enzyme(s)     Collagenase Type 2: 0.1%
Neutral Protease: 0.08%
Trypsin: 0.05%
Medium     Iscove modified DMEM


Heart: Human
  • Human
Avolio, E., Gianfranceschi, G., Cesselli, D., Caragnano, A., Athanasakis, E., Katare, R., Meloni, M., Palma, A., Barchiesi, A., Vascotto, C., Toffoletto, B., Mazzega, E., Finato, N., Aresu, G. and Livi, U.: Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction. , Stem Cells 32, 2373, 2014
 
Cells:      Cardiac stem
Enzyme(s)     Collagenase Type 2: 0.25%
Medium     Joklik modified Eagle’s


Heart: Human
  • Human
Voigt, N., Zhou, X. and Dobrev, D.: Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. , J Vis Exp , e50235, 2013
 
Cells:      Atrial myocytes
Enzyme(s)     Collagenase Type 1: 286 u/ml
Protease: 5 u/ml
Medium     see references


Heart: Human
  • Human
Vukusic, K., Jonsson, M., Brantsing, C., Dellgren, G., Jeppsson, A., Lindahl, A. and Asp, J.: High Density Sphere Culture of Adult Cardiac Cells Increases the Levels of Cardiac and Progenitor Markers and Shows Signs of Vasculogenesis. , Biomed Res Int 2013, , 2013
 
Cells:      Cardiac
Enzyme(s)     Collagenase Type 2: 0.08%
Medium     DMEM


Heart: Human
  • Human
Anderson, E., Rodriguez, E., Anderson, C., Thayne, K., Chitwood, W. and Kypson, A.: Increased Propensity for Cell Death in Diabetic Human Heart is Mediated by Mitochondrial-Dependent Pathways. , Am J Physiol Heart Circ Physiol 300, H118, 2011
 
Cells:      Myofibers
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     see reference


Heart: Human
  • Human
Voigt, N., Makary, S., Nattel, S. and Dobrev, D.: Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart. , Methods Enzymol 484, 653, 2010
 
Cells:      Atrial cardiomyocytes
Enzyme(s)     Collagenase Type 1: 286 u/ml
Protease: 5 u/ml
Medium     see reference


Heart: Human
  • Human
Jensen, B., Swigart, P., Laden, M., DeMarco, T., Hoopes, C. and Simpson, P.: The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries. , J Am Coll Cardiol 54, 1137, 2009
 
Cells:      Coronary artery smooth muscle
Enzyme(s)     Collagenase Type 2: 0.1%
Elastase: 0.05%
Medium     DMEM


Heart: Human
  • Human
Mathew, S., Tustison, K., Sugatani, T., Chaudhary, L., Rifas, L. and Hruska, K.: The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD. , J Am Soc Nephrol 19, 1092, 2008
 
Cells:      Smooth muscle aortic
Enzyme(s)     Collagenase Type 1: 0.2%
Collagenase Type 2: 0.1%
Elastase: 0.025%
Medium     DMEM


Heart: Human
  • Human (n=16, age: 60 &plusmin;3 years)
Mukerjee, R., Multani, M., Sample, J., Dowdy, K., Zellner, J., Hoover, D., and Spinale, F.: Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response , J Cardiovasc Pharmacol 7 (4), 235, 2002
 
Cells:      Myocardial
Enzyme(s)     Hyaluronidase: 0.05%
Medium     Medium 199


Heart: Human
  • Human
Todor Anastassia, Sharov Victor G, Tanhehco Elaine J, Silverman Norman, Bernabei Alvise, Sabbah Hani N: Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes , Am J Physiol Heart Circ Physiol 283, H990-5, 2002
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Collagenase Type 1: 0.025%
Protease XIV: 0.013%
Medium     HEPES solution


Heart: Human
  • Human
Hoppe, U., Jansen, E., Sudkamp, M., and Beukelmann, D.: Hyperpolarization-Activated Inward Current in Ventricular Myocytes From Normal and Failing Human Hearts , Circulation 97, 55, 1998
 
Cells:      Heart
Myocytes
Enzyme(s)     Collagenase Type 2: 200 u/ml
Medium     Tyrode's solution


Heart: Human
  • Human
Shirai, T., Rao, V., Weisel, R., Ikonomidis, J., Li, R., Tumiati, L., Merante, F. and Mickle, D.: Preconditioning Human Cardiomyocytes and Endothelial Cells. , J Thorac Cardiovasc Surg 115, 210-9, 1998
 
Cells:      Cardiomyocytes and endothelial
Enzyme(s)     Trypsin: 0.2%
Collagenase: 0.1%
Medium     PBS


Heart: Human
  • Human
Van Wagoner, D., Pond, A., McCarthy, P., Trimmer, J. and Nerbonne, J.: Outward K+ Current Densities and Kv1.5 Expression are Reduced in Chronic Human Atrial Fibrillation. , Circ Res 80, 772-81, 1997
 
Cells:      Atrial myocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Protease Type XIV: 0.04%
Medium     see reference


Heart: Human
  • Human, fetal, 14.5 week gestation
Goldman, B., and Wurzel, J.: Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes , In Vitro Cell Dev Biol 28, 109, 1992
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.1%
Medium     HBSS with Calcium


Heart: Human
  • Human, both sexes, 18-81 years
Smith, D., Glover, J., Townsend, L., and Maupin, E.: A Method for the Harvest, Culture, and Characterization of Human Adult Atrial Myocardial Cells: Correlation with Age of Donor , In Vitro Cell Dev Biol 27, 914, 1991
 
Cells:      Myocardial
Atrial
Enzyme(s)     Collagenase: 0.14%
Medium     HBSS, CMF


Heart: Human
  • Human, adult
Bugaisky, L.B.: Biology of Isolated Adult Cardiac Myocytes,Isolation and Culture of Human Adult Cardiac Myocytes,(Clark,Decker,Borg,eds), Elsevier Science Publishing Co., Inc., , 1988
 
Cells:      Myocytes
Enzyme(s)     Trypsin: 0.25%
Medium     Joklik's MEM


Heart: Human
  • Human
Hassler, O., Wiren, M., and Herbertsson, S.: The Elastic Coat of the Arterial Wall Studied with the Aid of Collagenase, Acta Pathol Microbiol Scand 57, 15, 1963
 
Cells:      Thoracic aorta
Enzyme(s)     Collagenase: 0.2%
Medium     Phosphate buffer w/NaCl


Heart: Invertebrate
  • Octopus, E. cirhosa, 260-352 g
Altimiras J., Hove-Madsen L., and Gesser H.: Ca(2+) Uptake in the Sarcoplasmic Reticulum from the Systemic Heart of Octopod Cephalopods , J Exp Biol 202, 2531, 1999
 
Cells:      Systemic heart cardiomyocytes
Enzyme(s)     Collagenase: 0.025%
Trypsin: 0.02%
Medium     see reference


Heart: Mouse
  • Mouse, male, 12-18 week
Jian, Z, Chen, Y, Shimkunas, R, Jian, Y, Jaradeh, M, Chavez, K, Chiamvimonvat, N, Tardiff, J, Izu, L, Ross, R and Chen-Izu, Y: In Vivo Cannulation Methods for Cardiomyocytes Isolation from Heart Disease Models. , PLoS ONE 11, e0160605, 2016
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 300 u/ml
Protease XIV: 0.004%
Medium     Perfusion solution (see reference)


Heart: Mouse
  • Mouse
Hu, D., Yin, C., Mohanta, S., Weber, C. and Habenicht, A.: Prepartaion of Single Cell Suspensions from Mouse Aorta , Bio Protoc 6, 11, 2016
 
Cells:      Aortic
Enzyme(s)     Collagenase Type 1: 400 u/ml
Collagenase: 120 u/ml
Hyaluronidase: 60 u/ml
Deoxyribonuclease I: 60 u/ml
Medium     DPBS


Heart: Mouse
  • Mouse, 4-6 week
Xu, J., Lee, Y., Ran, X., Liao, S., Yang, J., Au, K., Lai, W., Esteban, M., Tse, H.: Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration. , Stem Cells 34, 2693-2706, 2016
 
Cells:      Cardiospheres
Enzyme(s)     Collagenase Type 2: 0.4%
Neutral Protease: 0.4%
Medium     PBS


Heart: Mouse
  • Mouse, 15-30 gm
Henn, M., Janjua, M., Kanter, E., Makepeace, C., Schuessler, R., Nichols, C. and Lawton, J.: Adenosine Triphosphate-Sensitive Potassium Channel Kir Subunits Implicated in Cardioprotection by Diazoxide. , J Am Heart Assoc 4, , 2015
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.12%
Medium     see reference


Heart: Mouse
  • Mouse, 3-4 week
Wang, H., Chen, H., Feng, B., Wang, X., He, X., Hu, R., Yin, M., Wang, W., Fu, W. and Xu, Z.: Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue. , BMC Biotechnol 14, 75, 2014
 
Cells:      Cardiac progenitor
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Heart: Mouse
  • Mouse, adult
Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H and Pinto-d-O, P: Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms. , Stem Cell Res Ther 5, 5, 2014
 
Cells:      Cardiac Progenitor
Enzyme(s)     Collagenase Type 2: 600 u/ml
Deoxyribonuclease I: 60 u/ml
Medium     MEM


Heart: Mouse
  • Mouse
Zafiriou, M., Noack, C., Unsold, B., Didie, M., Pavlova, E., Fischer, H., Reichardt, H., Bergmann, M., El-Armouche, A., Zimmermann, W., Zelarayan, L.: Erythropoietin Responsive Cardiomyogenic Cells Contribute to Heart Repair Post Myocardial Infarction. , Stem Cells 32, 2480, 2014
 
Cells:      Cardiac progenitor
Enzyme(s)     Collagenase Type 2: 0.1%
Neutral Protease: 2.4 u/ml
Medium     HBSS


Heart: Mouse
  • Mouse, adult
Kohncke, C. Lisewski, U., Schleussner, L., Gaertner, C., Reichert, S. and Roepke, T.: Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes. , J Vis Exp 73, e50145, 2013
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.08%
Medium     see reference


Heart: Mouse
  • Mouse
Hennessey, J., Wei, E. and Pitt, G.: Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels. , Circ Res 113, 381-8, 2013
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 150 u/ml
Medium     MEM


Heart: Mouse
  • Mouse
Touchberry, C., Green, T., Tchikrizov, V., Mannix, J., Mao, T., Carney, B., Girgis, M., Vincent, R., Wetmore, L., Dawn, B., Bonewald, L., Stubbs, J. and Wacker, M.: FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy. , Am J Physiol Endocrinol Metab 304, E863, 2013
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 360 u/ml
Papain: 0.4 u/ml
Deoxyribonuclease I: 40 u/ml
Medium     L-15


Heart: Mouse
  • Mouse
Pratumvinit, B., Reesukumal, K., Janebodin, K., Ieronimakis, N. and Reyes, M.: Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells. , Biomed Res Int 2013, 359412, 2013
 
Cells:      Cardiac endothelial
Enzyme(s)     Collagenase Type 4: 0.2%
Neutral Protease: 1.2 u/ml
Medium     PBS


Heart: Mouse
  • Mouse, 2-6 month
Christel, C., Cardona, N., Mesirca, P., Herrmann, S., Hofmann, F., Striessnig, J., Ludwig, A., Mangoni, M. and Lee, A.: Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node. , J Physiol 590, 6327, 2012
 
Cells:      Sinoatrial node
Enzyme(s)     Collagenase Type 2: 229 u/ml
Elastase: 1.9 u/ml
Protease Type XIV: 0.9 u/ml
Medium     Tyrode's solution


Heart: Mouse
  • Mouse, male, 8-25 week
Carley, A. and Kleinfeld, A.: Fatty Acid (FFA) Transport in Cardiomyocytes Revealed by Imaging Unbound FFA is Mediated by an FFA Pump Modulated by the CD36 Protein. , J Biol Chem 286, 4589-97, 2011
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 59 u/ml
Medium     MEM


Heart: Mouse
  • Mouse
Butcher, M., Herre, M., Ley, K. and Galkina, E.: Flow Cytometry Analysis of Immune Cells Within Murine Aortas. , J Vis Exp 53, e2828, 2011
 
Cells:      Aortic adventitial leukocytes
Enzyme(s)     Collagenase Type 2: 300 u/ml
Elastase: 5.6 u/ml
Medium     PBS


Heart: Mouse
  • Mouse, adult
Flynn, J., Santana, L. and Melov, S.: Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes. , J Vis Exp 58, e3302, 2011
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 1: 0.1-0.4%
Protease Type XIV: 0.004-.02%
Medium     see reference


Heart: Mouse
  • Mouse, male, 6 week
Makino, A., Suarez, J., Wang, H., Belke, D., Scott, B. and Dillmann, W.: Thyroid Hormone Receptor-beta is Associated With Coronary Angiogenesis During Pathological Cardiac Hypertrophy. , Endocrinology 150, 2008, 2009
 
Cells:      Coronary endothelial cells
Enzyme(s)     Collagenase Type 2: 0.1%
Neutral Protease: 0.6 u/ml
Medium     M199


Heart: Mouse
  • Mouse, adult
Zhang, Y, Kanter, E., Laing, J., Aprhys, C., Johns, D., Kardami, E. and Yamada, K.: Connexin43 Expression Levels Influence Intercellular Coupling and Cell Proliferation of Native Murine Cardiac Fibroblasts. , Cell Commun Adhes 15, 289, 2008
 
Cells:      Ventricular myoctes
Enzyme(s)     Collagenase: 0.1%
Medium     M199


Heart: Mouse
  • Mouse, adult, 9-13 week
Kabaeva, Z., Zhao, M. and Michele, D.: Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. , Am J Physiol Heart Circ Physiol 294, H1667-74, 2008
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 620 u/ml
Protease XIV: 0.104 u/ml
Deoxyribonuclease I: 0.0015%
Medium     Myocyte buffer see reference


Heart: Mouse
  • Mouse, adult
O'Connell Timothy D, Rodrigo Manoj C, Simpson Paul C: Isolation and culture of adult mouse cardiac myocytes , Methods Mol Biol 357, 271-96, 2007
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase: see reference
Medium     HBSS


Heart: Mouse
  • Mouse
Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy , J Am Coll Cardiol 49, 706, 2007
 
Cells:      Smooth muscle cells
Enzyme(s)     Collagenase Type 2: 300 u/ml
Elastase: 5 u/ml
Medium     F10 Ham


Heart: Mouse
  • Mouse, adult, 6-12 week
Shioya, T: A Simple Technique for Isolating Healthy Heart Cells from Mouse Models. , J Physiol Sci 57, 327-35, 2007
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Trypsin: 0.006%
Protease XIV: 0.006%
Medium     see reference


Heart: Mouse
  • Mouse
Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms , J Physiol 575, 627-44, 2006
 
Cells:      Ventricular myocytes and mesenteric arterial SMC
Enzyme(s)     Collagenase Type 2: 0.06%
Papain: 0.175%
Medium     Krebs-Ringer


Heart: Mouse
  • Mouse
Chen, H., Yong, W., Ren, S., Shen, W., He, Y., Cox, K., Zhu, W., Li, W., Soonpaa, M., Payne, RM, Franco, D., Field, L., Rosen, V., Wang, Y. and Shou, W.: Overexpression of Bone Morphogenetic Protein 10 in Myocardium Disrupts Cardiac Postnatal Hypertrophic Growth. , J Biol Chem 281, 27481, 2006
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 1: 0.17%
Medium     PBS


Heart: Mouse
  • Mouse, neonatal
Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.: ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes , Cell Physiol Biochem 15, 263, 2005
 
Cells:      Cardiomyoctes
Enzyme(s)     Collagenase Type 1: 150 u/ml
Trypsin: 0.01%
Medium     M199


Heart: Mouse
  • Mouse, 1 day
Potts, Malia B., Vaughn, Allyson E., McDonough, Holly, Patterson, Cam, and Deshmukh, Mohanish: Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP , J Cell Biol 171, 925, 2005
 
Cells:      Cardiomyoctes
Enzyme(s)     NCIS kit: per instructions
Medium     EBSS


Heart: Mouse
  • Mouse
Kobayashi, M., Inoue, K., Warabi, E., Minami, T. and Kodama, T.: A Simple Method of Isolating Mouse Aortic Endothelial Cells. , J Atheroscler Thromb 12, 138, 2005
 
Cells:      Aortic endothelial
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM


Heart: Mouse
  • Mouse
Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori: Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques , Circulation 110, 1953-9, 2004
 
Cells:      Smooth muscle cells
Enzyme(s)     Collagenase Type 1: 0.1%
Elastase: 0.0125%
Medium     DMEM


Heart: Mouse
  • Mouse, female, 14-18 g
Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H: Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo , Circulation 110, 3803-7, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.02%
Elastase: 0.03%
Pancreatin: 0.06%
Medium     see reference


Heart: Mouse
  • Mouse, 6-8 week
Qian, Q., Hunter, L., Li, M., Marin-Padilla, M., Prakash, Y., Somlo, S., Harris, P., Torres, V., and Sieck, G.: Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells , Hum Mol Genet 12(15), 1875, 2003
 
Cells:      Vascular smooth muscle
Enzyme(s)     Papain: 10 u/ml
Elastase: .005%
Collagenase: 0.05%
Deoxyribonuclease I: 1000 u/ml
PDS kit: with modifications
Medium     EBSS


Heart: Mouse
  • Mouse
Rybkin, I., Markham, D., Yan, Z., Bassel-Duby, R., Williams, R., and Olson, E.: Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation , J Biol Chem 278(18), 15927, 2003
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.2%
Pancreatin: 0.06%
Medium     DMEM/F-12


Heart: Mouse
  • Mouse, neonatal 2-4 day
Bettahi, I,, Marker, C., Roman, M., and Wickman, K.: Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh , J Biol Chem 277, 48282, 2002
 
Cells:      Atrial myocytes
Enzyme(s)     NCIS kit: with modifications see reference
Medium     L-15


Heart: Mouse
  • Mouse, neonatal and adult
Schreiber, K., Paquet, L., Allen, B., and Rindt, H.: Protein Kinase C Isoform Expression and Activity in the Mouse Heart , Am J Physiol Heart Circ Physiol 281(5), H2062, 2001
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.03-0.2%
Medium     Joklik's MEM


Heart: Mouse
  • Mouse, NTg and Tg, 8 - 12 week
Nelson, D., Setser, E., Hall, D., Schwartz, S., Hewitt, T., Klevitsky, R., Osinska, H., Bellgrau, D., Duke, R., and Robbins, J.: Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart , J Clin Invest 105 (9), 1199, 2000
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 2: 150 u/ml
Medium     Joklik's MEM


Heart: Mouse
  • Mouse, 1-6 day
Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G, and Coles JG.: Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture , Transplantation 70, 1310, 2000
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Deoxyribonuclease I: 0.002%
Medium     PBS


Heart: Mouse
  • Mouse, 2-4 mo
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP: Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology , Am J Physiol Heart Circ Physiol 279, H429-36, 2000
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Collagenase Type 4: 0.05%
Protease XIV: 0.002%
Medium     MEM


Heart: Mouse
  • Mouse
Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR: High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates , Circulation 101, 178-84, 2000
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 150 u/ml
Medium     Joklik's MEM


Heart: Mouse
  • Mouse, 1-3 day
Wang, G., and Kang, Y.: Inhibition of Doxorubicin Toxicity in Cultured Neonatal Mouse Cardiomyocytes with Elevated Metallothionein Levels , J Pharmacol Exp Ther 288(3), 938, 1999
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.25%
Medium     FBS-MEM


Heart: Mouse
  • Mouse, 1 day
Lader, A., Kwiatkowski, D., and Cantiello, H.: Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels , Am J Physiol 277, C1277, 1999
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: with modifications see reference
Medium     L-15


Heart: Mouse
  • Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation , Mol Biol Cell 10, 1569-79, 1999
 
Cells:      Fibroblasts, mesangial, smooth muscle
Enzyme(s)     Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: 0.05%
Medium     DMEM


Heart: Mouse
  • Mouse (BALB/c), adult
Felzen, B., Shilkrut, M., Less, H., Sarapov, I., Maor, G., Coleman, R., Robinson, R., et al.: Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice , Circ Res 82, 438, 1998
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 2: 0.04%
Medium     Tyrode's solution


Heart: Mouse
  • Mouse, 1 day
Lader, A., Xiao, Y., Ishikawa, Y., Cui, Y., Vatner, D., Vatner, S., Homcy, C., Cantiello, H.: Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway , Proc Natl Acad Sci U S A 95, 9669, 1998
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: with modifications see reference
Medium     L-15


Heart: Mouse
  • Mouse, adult
Grupp, I., Lorenz, J., Walsh, R., Boivin, G. and Rindt, H.: Overexpression of Alpha1B-Adrenergic Receptor Induces Left Ventricular Dysfunction in the Absence of Hypertrophy. , Am J Physiol 275, H1338-50, 1998
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 1: 75 u/ml
Collagenase Type 2: 75 u/ml
Medium     Joklik's MEM


Heart: Mouse
  • Mouse, P0-P1
Arber, S., Hunter, J., Ross, J., Hongo, M., Sansig, G., Borg, J., Perriard, J., Chien, K., and Caroni, P.: MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure , Cell 88, 393, 1997
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase: 0.045%
Medium     DMEM, Medium 199


Heart: Mouse
  • Mouse, 6-9 or 21-41 day
Valenzuela, D., Han, X., Mende, U., Fankhauser, C., Mashimo, H., Huang, P., Pfeffer, J., Neer, E., and Fishman, M.: G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart , Proc Natl Acad Sci U S A 94(5), 1727, 1997
 
Cells:      Ventricular and atrial myocytes
Enzyme(s)     Collagenase Type 2: 95 u/ml
Hyaluronidase: 172.5 u/ml
Trypsin: 0.002%
Deoxyribonuclease I: 60u/ml
Medium     DMEM/Tyrodes


Heart: Mouse
  • Mouse
Soonpaa, M., Kim, K., Pajak, aL., Franklin, M., and Field, L.: Cardiomyocyte DNA Synthesis and Binucleation During Murine Development , Am J Physiol 271, H2183, 1996
 
Cells:      Cardiomyocyte
Enzyme(s)     Collagenase: 0.17 %
Medium     PBS


Heart: Mouse
  • Mouse embryos
Wobus, A., Kleppisch, T., Maltsev, V., and Hescheler, J.: Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels , In Vitro Cell Dev Biol 30A, 425, 1994
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Heart: Mouse
  • Mouse
    (also rat, neonate and chick, 8 day embryo)
Gross, W., Schopf-Ebner, E., and Bucher, O.: Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells , Exp Cell Res 53, 1, 1968
 
Cells:      Myocytes
Enzyme(s)     Trypsin NF 1:250: 0.25%
Medium     Rinaldini's buffer solution, CF


Heart: Ovine
  • Ovine
Voigt, N., Pearman, C., Dobrev, D. and Dibb, K.: Methods for Isolating Atrial Cells from Large Mammals and Humans. , J Mol Cell Cardiol 86, 187-98, 2015
 
Cells:      Atrial myocytes
Enzyme(s)     Collagenase Type 2: 0.055%
Protease XIV: 0.006%
Medium     Tyrode's solution


Heart: Ovine
  • Ovine
Dibb, K., Clarke, J., Horn, M., Richards, M., Graham, H., Eisner, D. and Trafford, A.: Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure. , Circ Heart Fail 2, 482-9, 2009
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.055%
Protease Type XIV: 0.006%
Medium     See reference


Heart: Ovine
  • Ovine
Dhamoon, A, Pandit, S., Sarmast, F., Parisian, K., Guha, P., Li, Y., Bagwe, S., Taffet, S. and Anumonwo, J.: Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. , Circ Res 94, 1332-9, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 100-200 u/ml
Medium     See reference


Heart: Porcine
  • Porcine
Butcher, J. and Nerem, R.: Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells. , J Heart Valve Dis 13, 478, 2004
 
Cells:      Aortic smooth muscle and intersitial cells
Enzyme(s)     Collagenase: 600 u/ml
Medium     PBS


Heart: Porcine
  • Porcine
Christ, M., Gunther, A., Heck, M., Schmidt, B., Falkenstein, E., and Wehling, M.: Aldosterone, Not Estradiol, Is the Physiological Agonist for Rapid Increases in cAMP in Vascular Smooth Muscle Cells , Circulation 99, 1485, 1999
 
Cells:      Coronary vascular smooth muscle (PCVSMCs)
Enzyme(s)     Trypsin: 0.037%
Medium     HEPES


Heart: Porcine
  • Miniature swine, adult, female, 25-40 kg, familiarized with treadmill exercise
Bowles, D., Hu, Q., Laughlin, M., and Sturek, M.: Exercise Training Increases L-type Calcium Current Density in Coronary Smooth Muscle , Am J Physiol 275 (44), H2159, 1998
 
Cells:      Coronary smooth muscle
Enzyme(s)     Trypsin: 0.1%
Medium     Krebs bicarbonate solution


Heart: Rabbit
  • Rabbit, New Zealand, 3-3.5 kg
Fowler, E., Wang, N., Hezzell, M., Chanoit, G., Hancox, J. and Cannell, M.: Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration , Proc Natl Acad Sci U S A 117, 2687-2692, 2020
 
Cells:      Cardiac Myocytes
Enzyme(s)     Protease XIV: 0.005%
Collagenase Type 1: 0.1-0.2%
Trypsin: 0.08%
Medium     HEPES


Heart: Rabbit
  • Rabbit, New Zealand, 2.3-2.9 kg
Farkas, A., Acsai, K., Nagy, N., Toth, A., Fulop, F., Seprenyi, G., Birinyi, P., Nanasi, P., Forster, T., Csanady, M., Papp, J., Varrro, A. and Farkas, A.: Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. , Br J Pharmacol 154, 93, 2008
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 1: 0.05%
Hyaluronidase: see reference
Protease: see reference
Medium     Krebs-Henseleit


Heart: Rabbit
  • Rabbit
Verheye, S., Martinet, W., Kockx, M., Knaapen, M., Salu, K., Timmermans, J., Ellis, J., Kilpatrick, D., DeMayer, G.: Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy , J Am Coll Cardiol 49, 706, 2007
 
Cells:      Smooth muscle cells
Enzyme(s)     Collagenase Type 2: 300 u/ml
Elastase: 5 u/ml
Medium     F10 Ham


Heart: Rabbit
  • Rabbit, New Zealand, 2 kg
Driesen Ronald B, Dispersyn Gerrit D, Verheyen Fons K, van den Eijnde Stefan M, Hofstra Leo, Thone Fred, Dijkstra Petra, Debie Wiel, Borgers Marcel, Ramaekers Frans C S: Partial cell fusion: a newly recognized type of communication between dedifferentiating cardiomyocytes and fibroblasts , Cardiovasc Res 68, 37-46, 2005
 
Cells:      Cardiomyocytes and fibroblasts
Enzyme(s)     Collagenase: 0.06%
Medium     Medium 199


Heart: Rabbit
  • Rabbit, New Zealand White, 2-3 kg
Spitzer, K., Ershler, P., Skolnick, R., and Vaughan-Jones, R.: Generation of Intracellular pH Gradients in Single Cardiac Myocytes with a Microperfusion System , Am J Physiol 278, H1371, 2000
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     HEPES


Heart: Rabbit
  • Rabbit, New Zealand, white, either sex
Sedarat, F., Xu, L., Moore, E., and Tibbits, G.: Colocalization of Dihydropyridine and Ryanodine Receptors in Neonate Rabbit Heart Using Confocal Microscopy , Am J Physiol Heart Circ Physiol 279, H202, 2000
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     EGTA-KB


Heart: Rabbit
  • Rabbit, adult 1.5-2.5 kg
Anumonwo JM, Delmar M, and Jalife J.: Electrophysiology of single heart cells from the rabbit tricuspid valve , J Physiol 425, 145, 1990
 
Cells:      Tricuspid valve cells
Enzyme(s)     Collagenase: 0.8 mg/ml
Medium     HEPES Tyrode solution


Heart: Rabbit
  • Rabbit, New Zealand, white, male, 2-3 kg
Buxton, I., and Brunton, L.: Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes , J Biol Chem 258 (17), 10233, 1983
 
Cells:      Cardiomyocytes
Enzyme(s)     Hyaluronidase: 0.5%
Medium     Eagle's MEM


Heart: Rabbit
  • Rabbit, adult, 2 kg
Dani, A., Cittadini, A., Flamini, G., Festuccia, G., and Terranova, T.: Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart , J Mol Cell Cardiol 9, 777, 1977
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.007%
Medium     Kreb's Ringer


Heart: Rat
  • Rat, adult
Zhang, Y., Wang, Y., Yanni, J., Qureshi, M., Logantha, S., Kassab, S., Boyett, M., Gardiner, N., Sun, H., Howarth, F. and Dobrzynski, H.: Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. , Front Physiol 10, 826, 2019
 
Cells:      Sinoatrial nodal
Enzyme(s)     Collagenase Type 4: 220 u/ml
Elastase: 1.4 u/ml
Protease: 0.45 u/ml
Medium     Tyrode solution


Heart: Rat
  • Rat, adult
Savi, M., Bocchi, L., Mena, P., Dall'Asta, M., Crozier, A., Brighenti, F., Stilli, D. and Del Rio, D.: In vivo Administration of Urolithin A and B Prevents the Occurrence of Cardiac Dysfunction in Streptozotocin-induced Diabetic Rats. , Cardiovasc Diabetol 16, 80, 2017
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Protease Type XIV: 0.01%
Medium     Tyrode solution


Heart: Rat
  • Rat, SD, 2 day
Abdul-Ghani, M., Suen, C., Jiang, B., Deng, Y., Weldrick, J., Putinski, C., Brunette, S., Fernando, P., Lee, T., Flynn, P., Leenen, F., Burgon, P. Stewart, D. and Megeney, L.: Cardiotrophin 1 Stimulates Beneficial Myogenic and Vascular Remodeling of the Heart. , Cell Res 27, 1195-1215, 2017
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 200 u/ml
Medium     DMEM


Heart: Rat
  • Rat, 250 g
Stoppel, W., Gao, A., Greaney, A., Partlow, B., Bretherton, R., Kaplan, D and Black, L: Elastic, Silk-Cardiac Extracellular Matrix Hydrogels Exhibit Time-Dependent Stiffening that Modulates Cardiac Fibroblast Response. , J Biomed Mater Res A 104, 3058, 2016
 
Cells:      Fibroblasts
Enzyme(s)     Collagenase Type 2: 300 u/ml
Medium     DMEM


Heart: Rat
  • Rat, 1-2 day
Xie, L., Pi, X., Townley-Tilson, WH., Li, N., Wehrens, X., Entman, M., Taffet, G., Mishra, A., Peng, J., Schisler, J., Meissner, G. and Patterson, C.: PHD2/3-dependent Hydroxylation Tunes Cardiac Response to β-adrenergic Stress via Phospholamban. , J Clin Invest 125, 2759-71, 2015
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     MEM


Heart: Rat
  • Rat, Wistar, 250-350g
Calderon-Sachez, E., Domi­nguez-Rodriguez, A., Lopez-Haldon, J., Jimenez-Navarro, M., Gomez, A., Smani, T. and Ordonez, A.: Cardioprotective Effect of Ranolazine in the Process of Ischemia-reperfusion in Adult Rat Cardiomyocytes. , Rev Esp Cardiol (Engl Ed) , , 2015
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 251 u/ml
Medium     Tyrode solution


Heart: Rat
  • Rat, SD, 8-10 week
Lee, J., Ha, J. and Leem, C.: A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca(2+) Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat. , Korean J Physiol Pharmacol 19, 373-82, 2015
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase: 0.08%
Pronase: 0.004%
Medium     Tyrode's Solution


Heart: Rat
  • Rat, SD, 1 day
Clark, A. and Naya, F.: MicroRNAs in the Myocyte Enhancer Factor 2 (MEF2)-regulated Gtl2-Dio3 Noncoding RNA Locus Promote Cardiomyocyte Proliferation by Targeting the Transcriptional Coactivator Cited2. , J Biol Chem 290, 23162-72, 2015
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.025%
Collagenase Type 2: 1.0%
Medium     HBSS


Heart: Rat
  • Rat, SD, 2-3 day
Fang, R, Qiao, S., Liu, Y., Meng, Q., Chen, X., Song, B., Hou, X. and Tian, W.: Sustained Co-Delivery of BIO and IGF-1 by a Novel Hybrid Hydrogel System to Stimulate Endogenous Cardiac Repair in Myocardial Infarcted Rat Hearts. , Int J Nanomedicine 10, 4691-703, 2015
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     DMEM


Heart: Rat
  • Rat, SD
Horne, T., VandeKopple, M., Sauls, K., Koenig, S., Anstine, L., Garg, V., Norris, R. and Lincoln, J.: Dynamic Heterogeneity of the Heart Valve Interstitial Cell Population in Mitral Valve Health and Disease, J Cardiovasc Dev Dis 2, 214, 2015
 
Cells:      Valve interstitial cells
Enzyme(s)     Collagenase Type 2: 600 u/ml
Medium     DMEM


Heart: Rat
  • Rat, Wistar, 200g
Miller, L., Greensmith, D., Sankaranarayanan, R., O'Neill, S. and Eisner D.: The Effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on Intracellular Ca2+ Handling in Rat Ventricular Myocytes. , Cell Calcium 58, 208-14, 2015
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 95 u/ml
Protease: 0.1 u/ml
Medium     HEPES


Heart: Rat
  • Rat, SD, 3-4 month
Liu, L., Wang, Y., Cao, Z., Wang, M., Liu, X., Gao, T., Hu, Q., Yuan, W. and Lin, L.: Up-regulated TLR4 in Cardiomyocytes Exacerbates Heart Failure after Long-Term Myocardial Infarction. , J Cell Mol Med 19, 2728, 2015
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Heart: Rat
  • Rat, neonatal
Song, D., Lee, K., Ryu, J., Jeon, H. and Kim, D.: The Molecular Interaction of Heart LIM Protein (HLP) with RyR2 and Caveolin-3 is Essential for Ca(2+)-Induced Ca(2+) Release in the Heart. , Biochem Biophys Res Commun 463, 975-81, 2015
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     HBSS


Heart: Rat
  • Rat, neonatal
Zhang, B., Montgomery, M., Davenport-Huyer, L., Korolj, A. and Radisic, M.: Platform Technology for Scalable Assembly of Instantaneously Functional Mosaic Tissues. , Sci. Adv. 1, e1500423, 2015
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.06%
Collagenase Type 2: 220 u/ml
Medium     HBSS


Heart: Rat
  • Rat, SD, 1-2 day
Rutering, J., Ilmer, M., Recio, A., Coleman, M., Vykoukal, J. and Alt, E.: Improved Method for Isolation of Neonatal Rat Cardiomyocytes with Increased Yield of C-Kit+ Cardiac Progenitor Cells. , J Stem Cell Res Ther 5, 1-8, 2015
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.12%
Medium     DMEM/F-12


Heart: Rat
  • Rat, neonatal
Sakurai, T., Lanahan, A., Woolls, M., Li, N., Tirziu, D. and Murakami, M.: Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction using Confocal Spinning Disk Microscopy. , J Vis Exp , e51666, 2014
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, adult, 300-370 g
Chouchani, E., Pell, V., Gaude, E., Aksentijevic, D., Sundier, S., Robb, E., Logan, A., Nadtochiy, S., Ord, E., Smith, A., Eyassu, F., Shirley, R., Hu, C., Dare, A., James, A. and Rogatti, S.: Ischaemic Accumulation of Succinate Controls Reperfusion Injury Through Mitochondrial ROS. , Nature 515, 431-435, 2014
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     KH buffer


Heart: Rat
  • Rat
Hennessey, J., Wei, E. and Pitt, G.: Fibroblast Growth Factor Homologous Factors Modulate Cardiac Calcium Channels. , Circ Res 113, 381-8, 2013
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 150 u/ml
Medium     MEM


Heart: Rat
  • Rat, Wistar, 175-200g
Eckerle, L., Felix, S. and Herda, L.: Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes. , J Vis Exp 73, e4237, 2013
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 150 u/ml
Medium     see reference


Heart: Rat
  • Rat, SD, 1-2 day
Zhang, B., Green, J., Murthy, S. and Radisic, M.: Label-Free Enrichment of Functional Cardiomyocytes using Microfluidic Deterministic Lateral Flow Displacement. , PLoS ONE 7, e37619, 2012
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.06%
Collagenase Type 2: 220 u/ml
Medium     DMEM


Heart: Rat
  • Rat, adult
Karakikes, I., Hadri, L., Rapti, K., Ladage, D., Ishikawa, K., Tilemann, L., Yi, G., Morel, C., Gwathmey, J., Zsebo, K., Weber, T., Kawase, Y. and Hajjar, R.: Concomitant Intravenous Nitroglycerin with Intracoronary Delivery of AAV1.SERCA2a Enhances Gene Transfer in Porcine Hearts. , Mol Ther 20, 565, 2012
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     Medium 199


Heart: Rat
  • Rat, 3 day
Ye, K., Sullivan, K. and Black, L.: Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering. , J Vis Exp 55, 3251, 2011
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 300 u/ml
Medium     PBS


Heart: Rat
  • Rat, SD, male
Mellor, K., Bell, J., Wendt, I., Davidoff, A., Ritchie, R. and Delbridge, L.: Fructose Modulates Cardiomyocyte Excitation-Contraction Coupling and Ca Handling In Vitro. , PLoS ONE 6, e25204, 2011
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.056%
Medium     Krebs


Heart: Rat
  • Rat, neonatal
Dittami, G., Rajguru, S., Lasher, R., Hitchcock, R. and Rabbitt, R.: Intracellular Calcium Transients Evoked by Pulsed Infrared Radiation in Neonatal Cardiomyocytes. , J Physiol 589, 1295-306, 2011
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, neonatal
Guan, X., Delo, D., Atala, A. and Soker, S.: In Vitro Cardiomyogenic Potential of Human Amniotic Fluid Stem Cells. , J Tissue Eng Regen Med 5, 220, 2011
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.07%
Collagenase Type 2: 0.1%
Medium     DMEM/M199


Heart: Rat
  • Rat, adult
Louch, W., Sheehan, K. and Wolska, B.: Methods in Cardiomyocyte Isolation, Culture, and Gene Transfer. , J Mol Cell Cardiol 51, 288-98, 2011
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.025%
Medium     see reference


Heart: Rat
  • Rat, Wistar, male
Brinckmann, M., Kaschina, E., Altarche-Xifro, W., Curato, C., Timm, M., Grzesiak, A., Dong, J., Kappert, K., Kintscher, U., Unger, T. and Li, J.: Estrogen Receptor Alpha Supports Cardiomyocytes Indirectly Through Post-Infarct Cardiac c-kit+ Cells. , J Mol Cell Cardiol 47, 66, 2009
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, neonatal
Smith, M., Huang, Y. and Deshmukh, M.: Skeletal Muscle Differentiation Evokes Endogenous XIAP to Restrict the Apoptotic Pathway. , PLoS ONE 4, e5097, 2009
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, adult
Xu, X. and Colecraft, H.: Primary Culture of Adult Rat Heart Myocytes. , J Vis Exp 28, 1308, 2009
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Protease XIV: 0.02%
Medium     Media 199


Heart: Rat
  • Rat, SD, 200-250 g
Farkas, A., Acsai, K., Nagy, N., Toth, A., Fulop, F., Seprenyi, G., Birinyi, P., Nanasi, P., Forster, T., Csanady, M., Papp, J., Varrro, A. and Farkas, A.: Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. , Br J Pharmacol 154, 93, 2008
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 1: 0.05%
Hyaluronidase: see reference
Protease: see reference
Medium     Krebs-Henseleit


Heart: Rat
  • Rat, SD, 1-2 day
Kim, M., Oh, J., Sakata, S., Liang, I., Park, W., Hajjar, R. and Lebeche, D.: Role of Resistin in Cardiac Contractility and Hypertrophy. , J Mol Cell Cardiol 45, 270, 2008
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, SD, 2 day
Jang, J., Ku, S., Kim, J., Choi, K., Kim, Y., Kim, H., Oh, S., Lee, E., Cho, H., Song, Y., Lee, S., Lee, S., Suh, C., Kim, S., Moon, S. and Choi, Y.: Notch Inhibition Promotes Human Embryonic Stem Cell-Derived Cardiac Mesoderm Differentiation. , Stem Cells 26, 2782, 2008
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM/F-12


Heart: Rat
  • Rat, SD, male, 225-250g
Kubli, D., Quinsay, M., Huang, C., Lee, Y., Gustafsson, A.: Bnip3 Functions as a Mitochondrial Sensor of Oxidative Stress During Myocardial Ischemia and Reperfusion. , Am J Physiol Heart Circ Physiol 295, H2025, 2008
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     J-MEM


Heart: Rat
  • Rat, 2-3 day
LaFramboise, W., Scalise, D., Stoodley, P., Graner, S., Guthrie, R., Magovern, J and Becich, M.: Cardiac Fibroblasts Influence Cardiomyocyte Phenotype in Vitro. , Am J Physiol Cell Physiol 292, C1799-808, 2007
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Pancreatin: 0.1%
Medium     DMEM


Heart: Rat
  • Rat, neonatal
Robinet, A., Millart, H., Oszust, F., Hornebeck, W. and Bellon, G.: Binding of Elastin Peptides to S-Gal Protects the Heart Against Ischemia/Reperfusion Injury by Triggering the RISK Pathway. , FASEB J 21, 1968, 2007
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat
Jenkins, C., Milsted, A., Doane, K., Meszaros, G., Toot, J. and Ely, D.: A Cell Culture Model using Rat Coronary Artery Adventitial Fibroblasts to Measure Collagen Production. , BMC Cardiovasc Disord 7, 13, 2007
 
Cells:      Ventricular fibroblasts
Enzyme(s)     Trypsin: 0.06%
Collagenase: 100 u/ml
Medium     ADS Buffer:see reference


Heart: Rat
  • Rat, SD, 300-350 g
Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA: An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction , J Exp Med 203, 53-61, 2006
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Hyaluronidase: 0.02%
Medium     DMEM


Heart: Rat
  • Rat, SD, male
Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M: Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation , FASEB J 20, 744-6, 2006
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase: 0.13%
Hyaluronidase: 0.06%
Medium     DMEM


Heart: Rat
  • Rat, SD, 200-250 g
Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi: Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms , J Physiol 575, 627-44, 2006
 
Cells:      Ventricular myocytes and mesenteric arterial SMC
Enzyme(s)     Collagenase Type 2: 0.06%
Papain: 0.175%
Medium     Krebs-Ringer


Heart: Rat
  • Rat, Wistar, neonatal
Butler, T., Au, C., Yang, B., Egan, J., Tan, Y., Hardeman, E., North, K., Verkman, A. and Winlaw, D.: Cardiac Aquaporin Expression in Humans, Rats, and Mice. , Am J Physiol Heart Circ Physiol 291, H705, 2006
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, 1-2 day
Pedram, A., Razandi, M., Aitkenhead, M., and Levin, E: Estrogen Inhibits Cardiomyocyte Hypertrophy in vitro. Antagonism of Calcineurin-related Hypertrophy through Induction of MCIP1 , J Biol Chem 280, 26339, 2005
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     DMEM/F-12


Heart: Rat
  • Rat, SD, male, 250-300 g
Head, B., Patel, H., Roth, D., Lai, N., Niesman, I., Farquhar, M. and Insel, P.: G-Protein-Coupled Receptor Signaling Components Localize in Both Sarcolemmal and Intracellular Caveolin-3-Associated Microdomains in Adult Cardiac Myocytes. , J Biol Chem 280, 31036-44, 2005
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 250 u/ml
Medium     Cardioplegic solution


Heart: Rat
  • Rat, Wistar, 1-3 day
Castillo, A., Ruzmetov, N., Harvey, K., Stillwell, W., Zaloga, G. and Siddiqui, R.: Docosahexaenoic Acid Inhibits Protein Kinase C Translocation/Activation and Cardiac Hypertrophy in Rat Cardiomyocytes. , J Mol Genet Med 1, 18, 2005
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, SD, 2-4 day
Entcheva, E., Bien, H, Yin L., Chung, C., Farrell, M., and Kostov, Y.: Functional Cardiac Cell Constructs on Cellulose-based Scaffolding , Biomaterials 25(26), 5753, 2004
 
Cells:      Cardiomyocytes, fibroblasts
Enzyme(s)     Trypsin: 0.1%
Collagenase: 0.1%
Medium     Medium 199


Heart: Rat
  • Rat, SD, 1-2 day
Muller-Bore, B., Cascio, W., Anderson, P., Snowwaert, J., Frye, J., Desai, N., Esch, G., Brackham, J., Bagnell, C., Coleman, W., Grisham, J., and Malouf, N.: Adult-derived Liver Stem Cells Acquire a Cardiomyocyte Structural and Functional Phenotype ex vivo , Am J Pathol 165, 135, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat,1-2 day
Natarajan AR, Rong Q, Katchman AN, and Ebert SN.: Intrinsic cardiac catecholamines help maintain beating activity in neonatal rat cardiomyocyte cultures , Pediatr Res 56, 411, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     DMEM


Heart: Rat
  • Rat, 1 day
Chen, Z., Ge, Y., and Kang J.: Down-regulation of the M6P/IGF-II Receptor Increases Cell Proliferation and Reduces Apoptosis in Neonatal Rat Cardiac Myocytes , BMC Cell Biol 5, 15, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     F-10


Heart: Rat
  • Rat, neonatal
Chen Hsiao-Huei, Mullett Steven J, Stewart Alexandre F R: Vgl-4, a novel member of the vestigial-like family of transcription cofactors, regulates alpha1-adrenergic activation of gene expression in cardiac myocytes , J Biol Chem 279, 30800-6, 2004
 
Cells:      Cardiac myocytes
Enzyme(s)     Collagenase Type 2: 0.5%
Medium     HBSS


Heart: Rat
  • Rat, male, 250-300 g
Hunton DaciaL, Zou LuYun, Pang Yi, Marchase Richard B: Adult rat cardiomyocytes exhibit capacitative calcium entry , Am J Physiol Heart Circ Physiol 286, H1124-32, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 1: 0.03%
Protease: 0.01%
Medium     HBSS


Heart: Rat
  • Rat, neonatal
Tamamori-Adachi, M., Hayashida, K., Nobori, K., Omizu, C., Yamada, K., Sakamoto, N., Kamura, T., Fukuda, K., Ogawa, S., Nakayama, K. and Kitajima, S.: Down-Regulation of p27 Promotes Cell Proliferation of Rat Neonatal Cardiomyocytes Induced by Nuclear Expression of Cyclin D1 and CDK4 , J Biol Chem 2279, 50429, 2004
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Heart: Rat
  • Rat, 50 g
Aberle II NS, and Ren J.: Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy , Biol Proced Online 5, 1, 2003
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase: 223 u/ml
Hyaluronidase: 0.01%
Trypsin: 0.002%
Medium     Medium 199


Heart: Rat
  • Rat, neonatal
Li TS, Ito H, Kajiwara K, and Hamano K.: Long-term survival of xenografted neonatal cardiomyocytes by adenovirus-mediated CTLA4-Ig expression and CD40 blockade , Circulation 108, 1760, 2003
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, adult, 220-280g
Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H: Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes , Am J Physiol Heart Circ Physiol 284, H330-6, 2003
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 178 u/ml
Hyaluronidase: 0.01%
Medium     Krebs-Henseleit, CF


Heart: Rat
  • Rat, fetal or 3-day
Tamamori-Adachi Mimi, Ito Hiroshi, Sumrejkanchanakij Piyamas, Adachi Susumu, Hiroe Michiaki, Shimizu Masato, Kawauchi Junya, Sunamori Makoto, Marumo Fumiaki, Kitajima Shigetaka, Ikeda Masa-Aki: Critical role of cyclin D1 nuclear import in cardiomyocyte proliferation , Circ Res 92, e12-9, 2003
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     HEPES


Heart: Rat
  • Rat, SD, 200-300 g
Swift Luther, McHowat Jane, Sarvazyan Narine: Inhibition of membrane-associated calcium-independent phospholipase A2 as a potential culprit of anthracycline cardiotoxicity , Cancer Res 63, 5992-8, 2003
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05-0.1%
Medium     Joklik's MEM


Heart: Rat
  • Rat, SD, 175-200g
Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise: Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation , Am J Physiol/Endo 284, E1055-64, 2003
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Deoxyribonuclease I: 0.02%
Medium     Krebs-Ringer


Heart: Rat
  • Rat, SD, pregnant female
DeAlmeida, A., Mustin, D., Forman, M., Brower, G., Janicki, J., and Carver, W.: Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression , J Cell Physiol 191, 51, 2002
 
Cells:      Peritoneal mast
Enzyme(s)     Hyaluronidase: 100 u/ml
Medium     DMEM (see reference)


Heart: Rat
  • Rat, neonatal, adult, 17 day
Lam ML, Bartoli M, and Claycomb WC.: The 21-day postnatal rat ventricular cardiac muscle cell in culture as an experimental model to study adult cardiomyocyte gene expression , Mol Cell Biochem 229, 51, 2002
 
Cells:      Ventricular cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.7-1%
Medium     DMEM


Heart: Rat
  • Rat, 1 day
Yang Y, Liao H, Ke Q, Cai J, Xiao YF, and Morgan JP: Enhancement of nitric oxide production by methylecgonidine in cultured neonatal rat cardiomyocytes , Br J Pharmacol 135, 188, 2002
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, female, 250-300 g
Liu Qinghang, Hofmann Polly A: Antiadrenergic effects of adenosine A(1) receptor-mediated protein phosphatase 2a activation in the heart , Am J Physiol Heart Circ Physiol 283, H1314-21, 2002
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     Ringer solution


Heart: Rat
  • Rat, Wistar, 1 day
Shimizu Tatsuya, Yamato Masayuki, Isoi Yuki, Akutsu Takumitsu, Setomaru Takeshi, Abe Kazuhiko, Kikuchi Akihiko, Umezu Mitsuo, Okano Teruo: Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces , Circ Res 90, e40, 2002
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 80u/ml
Medium     M199


Heart: Rat
  • Rat, male, 6-12 months old
Kitta, K., Day, R., Ikeda, T., and Suzuki, Y.: Hepatocyte Growth Factor Protects Cardiac Myocytes Against Oxidative Stress-Induced Apoptosis , Free Radic Biol Med 31 (7), 902, 2001
 
Cells:      Cardiac myocytes
Enzyme(s)     Protease: 0.55 u/ml
Medium     Tyrode's solution


Heart: Rat
  • Rat, Harlan SD, pup
Schwartzbauer, G., and Robbins, J.: The Tumor Suppressor Gene PTEN Can Regulate Cardiac Hypertrophy and Survival , J Biol Chem 276, 35786, 2001
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin:
Medium     DMEM/Ham's F-12


Heart: Rat
  • Rat, SD, newborn
Rahman A, Alam M, Rao S, Cai L, Clark LT, Shafiq S, and Siddiqui MA: Differential effects of doxorubicin on atrial natriuretic peptide expression in vivo and in vitro , Biol Res 34(3-4), 195, 2001
 
Cells:      Cardiac myocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, adult
Ren J, and Wold LE.: Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging , Biol Proced Online 3, 43, 2001
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase: 223 u/ml
Hyaluronidase: 0.01%
Trypsin: 0.002%
Medium     Tyrode solution


Heart: Rat
  • Rat, SD, male
Dai L, Brookes PS, Darley-Usmar VM, and Anderson PG.: Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of NO , Am J Physiol/Heart 281, H2261-H2269, 2001
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 140 u/ml
Medium     Krebs-Henseleit


Heart: Rat
  • Rat, neonatal
Guo K, Searfoss G, Krolikowski D, Pagnoni M, Franks C, Clark K, Yu KT, Jaye M, Ivashchenko Y: Hypoxia induces the expression of the pro-apoptotic gene BNIP3 , Cell Death Differ 8, 367-76, 2001
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     DMEM/M199


Heart: Rat
  • Rat, Wistar, female
Pyle, W, Lester, J, and Hofmann, P.: Effects of Kappa-opioid receptor activation of myocardium , Am J Physiol Heart Circ Physiol 281, H669, 2001
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     Ringer solution


Heart: Rat
  • Rat, SD, 1-2 day
Arutunyan A, Webster D, Swift L, Sarvazyan N: Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias , Am J Physiol Heart Circ Physiol 280, H1905-15, 2001
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.01%
Collagenase Type 2: 0.08%
Medium     HBSS


Heart: Rat
  • Rat, newborn
Kim, D., and Pleumsamran, A.: Cytoplasmic Unsaturated Free Fatty Acids Inhibit ATP-Dependent Gating of the G Protein-gated K+ Channel , J Gen Physiol 115, 287, 2000
 
Cells:      Atrial
Enzyme(s)     Trypsin: 0.06%
Medium     DMEM


Heart: Rat
  • Rat, 1-2 day
Bueno OF, De Windt LJ, Tymitz KM, Witt SA, Kimball TR, Klevitsky R, Hewett TE, Jones SP, Lefer DJ, Peng CF, Kitsis RN, and Molkentin JD.: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice , EMBO J 19(23), 6341, 2000
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 1: 84 u/ml
Pancreatin: 0.05%
Medium     M199 medium


Heart: Rat
  • Rat, Winstar, 1-2 day
Maki T, Horio T, Yoshihara F, Suga S, Takeo S, Matsuo H, and Kangawa K: Effect of neutral endopeptidase inhibitor on endogenous atrial natriuretic peptide as a paracrine factor in cultured cardiac fibroblasts , Br J Pharmacol 131(6), 1204, 2000
 
Cells:      Ventricular myocytes
Enzyme(s)     Collagenase Type 2: 0.08%
Medium     DMEM


Heart: Rat
  • Rat, SD, adult female
Puri S, Bansal DD, Uskokovic MR, and MacGregor RR.: Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) , Am J Physiol/Endo 278(2), E293, 2000
 
Cells:      Heart microvascular cells
Enzyme(s)     CLSPA: 250u/ml
Papain: 5 u/ml
Elastase: 0.8 u/ml
Medium     L-15


Heart: Rat
  • Rat, SD, male, 250-300g
Sun L, Chang J, Kirchhoff SR, and Knowlton AA.: Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment , Am J Physiol Heart Circ Physiol 278(4), H1091, 2000
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.06%
Medium     M199


Heart: Rat
  • Rat, wistar, 2 day
Cowan DB, Poutias DN, Del Nido PJ, and McGowan FX Jr: CD14-independent activation of cardiomyocyte signal transduction by bacterial endotoxin , Am J Physiol Heart Circ Physiol 279, H619, 2000
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     DMEM-F12


Heart: Rat
  • Rat, neonatal
Webster DR, and Patrick DL.: Beating rate of isolated neonatal cardiomyocytes is regulated by the stable microtubule subset , Am J Physiol Heart Circ Physiol 278, H1653, 2000
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     L-15


Heart: Rat
  • Rat, Lewis, adult male, 300-350-g
Kim, B., Nikolovski, J., Bonadio, J., Smiley, E., and Mooney, D.: Engineered Smooth Muscle Tissues: Regulating Cell Phenotype with the Scaffold , Exp Cell Res 251, 318, 1999
 
Cells:      Aorta, smooth muscle
Enzyme(s)     Trypsin: 0.025%
Medium     Medium 199 with 20% FBS medium


Heart: Rat
  • Rat, 10-15 newborn, 1-2 days old
Nemec, J., Wickman, K., and Clapham, D.: GBy Binding Increases the Open Time of IKACh: Kinetic Evidence for Multiple GBy Binding Sites , Biophys J 76, 246, 1999
 
Cells:      Atrial
Enzyme(s)     Trypsin: 0.03%
Medium     HBSS or EBSS


Heart: Rat
  • Rat, 1-3 day
Adderley SR, and Fitzgerald DJ.: Oxidative damage of cardiomyocytes is limited by extracellular regulated kinases 1/2-mediated induction of cyclooxygenase-2 , J Biol Chem 274, 5038, 1999
 
Cells:      Neonatal rat cardiomyocytes
Enzyme(s)     Collagenase Type 2: 80u/ml
Pancreatin: 0.06%
Medium     DMEM/F12


Heart: Rat
  • Rat, 1-2 day
Reinecke H, Zhang M, Bartosek T, and Murry CE.: Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts , Circulation 100(2), 193, 1999
 
Cells:      Cardiomyocytes
Enzyme(s)     Pancreatin: 0.06%
Collagenase Type 2: 95 u/ml
Medium     DMEM


Heart: Rat
  • Rat, adult and neonatal
Richards SM, Jaconi ME, Vassort G, and Puceat M.: A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes , J Cell Sci 112, 1519, 1999
 
Cells:      Cardioomyocytes
Enzyme(s)     Collagenase Type 4: 0.12%-adult
Collagenase Type 2: 0.05%-neonatal
Medium     DMEM/medium 199


Heart: Rat
  • Rat, Wistar, male
Sambandam N, Abrahani MA, St Pierre E, Al-Atar O, Cam MC, and Rodrigues B.: Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin , Arterioscler Thromb Vasc Biol 19(6), 1526, 1999
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 228 u/ml
Medium     Joklik MEM


Heart: Rat
  • Rat, 1-2 day
Matsui, T., Li, Ling, Del Monte, F., Fukui, Y., Franke, T., Hajjar, R., Rosenzweig, A.: Adenoviral gene transfer of activated phosphatidylinositol 3'-kinase and Akt inhibits apoptosis of hypoxic cardiomyocytes in vitro , Circulation 100, 2373-9, 1999
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     RPMI


Heart: Rat
  • Rat
Zilberman, A., Dave, V., Miano, J., Olson, E., and Periasamy, M.: Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression , Circ Res 82, 566, 1998
 
Cells:      Thoracic aorta, smooth muscle
Enzyme(s)     Elastase: 0.02%
Medium     (see reference)


Heart: Rat
  • Rat, SD, 1 day
Wagner DR, Combes A, McTiernan C, Sanders VJ, Lemster B, Feldman AM: Adenosine inhibits lipopolysaccharide-induced cardiac expression of tumor necrosis factor-alpha , Circ Res 82, 47-56, 1998
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     DMEM/F-12


Heart: Rat
  • Rat, SD, 300-350 g
Yu L, Netticadan T, Xu YJ, Panagia V, Dhalla NS: Mechanisms of lysophosphatidylcholine-induced increase in intracellular calcium in rat cardiomyocytes , J Pharmacol Exp Ther 286, 1-8, 1998
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.04%
Medium     see reference


Heart: Rat
  • Rat, SD, female, 200g
Westfall M V, Rust E M, Metzger J M: Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function , Proc Natl Acad Sci U S A 94, 5444-9, 1997
 
Cells:      Ventricular cardiac myocytes
Enzyme(s)     Collagenase Type 2: 0.5%
Hyaluronidase: 0.2%
Medium     Krebs-Henseleit buffer


Heart: Rat
  • Rat, Wistar, 1 day
Kinugawa, K., Kohmoto, O., Yao, A., Serizawa, T., and Takahashki, T.: Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes , Am J Physiol 272, H35-47, 1997
 
Cells:      Cardiomyoctes
Enzyme(s)     Collagenase Type 2: 80 u/ml
Medium     HBSS


Heart: Rat
  • Rat, neonatal, 5-day
Vanwinkle, W., Snuggs, M., and Buja, L.: Cardiogel: A Biosynthetic Extracellular Matrix for Cardiomyocyte Culture , In Vitro Cell Dev Biol Anim 32, 478, 1996
 
Cells:      Cardiomyocytes
Enzyme(s)     Trypsin: 0.05%
Medium     DMEM


Heart: Rat
  • Rat, SD, 250-300 g
Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH, and Sheu SS: Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes , Circ Res 79(1), 86, 1996
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     Joklik's


Heart: Rat
  • Rat, SD, fetal and neonatal
Li RK, Mickle DA, Weisel RD, Zhang J, Mohabeer MK: In vivo survival and function of transplanted rat cardiomyocytes , Circ Res 78, 283-8, 1996
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase: 0.1%
Trypsin: 0.2%
Medium     PBS


Heart: Rat
  • SD, SHR or WKY
MacGregor, R., Klein, R., and Bansal, D.: Secretion of Plasminogen Activator Activity from Neonatal Rat Heart Cells is Regulated by Hormones and Growth Factors , Ann N Y Acad Sci 752, 331, 1995
 
Cells:      Cardiomyocytes
Enzyme(s)     NCIS kit: per instructions
Medium     HBSS


Heart: Rat
  • Rat, SD, male, 200-250 g
Laughlin, M., Schaeffer, M., and Sturek, M.: Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats , Am J Physiol 73, 1141, 1992
 
Cells:      Myocytes
Ventricles
Enzyme(s)     Collagenase: 0.07%
Medium     Joklik's MEM


Heart: Rat
  • Rat, SD, female, 60-90 days
Welder, A.A., Grant, R., Bradlaw, J., and Acosta, D.: A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents , In Vitro Cell Dev Biol 27, 921, 1991
 
Cells:      Myocardial
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     Joklik's MEM, Kreb's-Henseleit buffer, CF


Heart: Rat
  • Rat, SD, adult, female, 180-220 g
Fischer, Y., Rose, H., and Kammermeier. H.: Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method , Life Sci 49, 1679, 1991
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase: 0.11%
Medium     (see reference)


Heart: Rat
  • Rat, neonatal, 1-4 days
Marino, T.A., Walter, R.,Cobb, E., Palasiuk, M., Parsons, T., and Mercer, W.E.: Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation , In Vitro Cell Dev Biol 26, 229, 1990
 
Cells:      Cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.12%
Medium     Krebs-Henseleit Buffer, CF


Heart: Rat
  • Rat
    (also bovine)
Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons , In Vitro Cell Dev Biol 26, 335, 1990
 
Cells:      Heart
Adrenal chromaffin
Paraneurons
Enzyme(s)     Trypsin: 0.06%
Medium     25mM HEPES buffered Locke's solution, CMF


Heart: Rat
  • Rat, adult, female
Nag, A.C., Lee, M., and Kosiur, J.R.: Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms , In Vitro Cell Dev Biol 26, 464, 1990
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     Joklik's MEM


Heart: Rat
  • Rats, AW, late-gestation pregnant females
Engelmann, G., McTiernan, C., Gerrity, R., and Samarel, A.: Serum-free Primary Cultures of Neonatal Rat Cardiomyocytes: Cellular and Molecular Applications, Technique 2 (6), 279, 1990
 
Cells:      Ventricular cardiomyocytes
Enzyme(s)     Collagenase Type 2: 0.05 - 0.08%
Medium     See reference


Heart: Rat
  • Rat,SD, albino, 10-14 weeks
Berg, I., Guse, A.H., and Gercken, G.: Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats , Biochim Biophys Acta 1010, 100, 1989
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 1: 100 u/ml and 150 u/ml
Medium     Krebs Ringer bicarbonate buffer


Heart: Rat
  • Rat, SD, adult, female, 175 - 225 g
Bugaisky, L.B. and Zak, R.: Differentiation of Adult Rat Cardiac Myocytes in Cell Culture , Circ Res 64, 493, 1989
 
Cells:      Myocytes
Enzyme(s)     Collagenase: 0.1%
Medium     Joklik's medium


Heart: Rat
  • Rat, SD, male, 250 - 350 g
De Young, M., Giannattasio, B., and Scarpa, A.: Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart ,Methods Enzymology Vol. 173,, , 662, 1989
 
Cells:      Myocytes, ventricular
Enzyme(s)     Collagenase Type 1: 90 and 100 u/ml
Medium     Joklik's solution with and without calcium


Heart: Rat
  • Rat, SD, 2-4 day old
Toraason, M., Luken, M., Breitenstein, M., Krueger, J., and Biagini, R.: Comparative Toxicity of Allylamine and Acrolein in Cultured Myocytes and Fibroblasts from Neonatal Rat Heart , Toxicology 56, 107, 1989
 
Cells:      Cardiomyocytes
Fibroblasts
Enzyme(s)     Trypsin: 0.1%
Medium     HBSS


Heart: Rat
  • Rat, SD, male
Cornwell, T., and Lincoln, T.: Regulation of Intracellular Ca2+ Levels in Cultured Vascular Smooth Muscle Cells , J Biol Chem 264 (2), 1146, 1989
 
Cells:      Aortic smooth muscle
Enzyme(s)     Trypsin: 0.05%
Medium     DMEM


Heart: Rat
  • Rat, adult, 150-200 g
McMahon, K.K.: A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, Eds), Elsevier Science Publishing Co. Inc., 318, 1988
 
Cells:      Myocytes, atria
Enzyme(s)     Collagenase Type 1: 100 u/ml
Medium     Kreb's Ringer bicarbonate - HEPES buffer


Heart: Rat
  • Rat, SD
Buxton, I.L.O. and Doggwiler, K.O.: Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), , 298, 1988
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     Kreb's Ringer w/ calcium


Heart: Rat
  • Rat, SD, male, 250-350 g
Kim, D. and Smith, T.W.: Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes,Biology of Isolated Adult Cardiac Myocytes,(Clark, Decker, Borg, eds), Elsevier Science Publishing Co. Inc., 370, 1988
 
Cells:      Myocytes, heart
Enzyme(s)     Hyaluronidase: 0.03%
Medium     Bicarbonate-buffered medium


Heart: Rat
  • Rat, Wistar, 2-4 day neonates
Grynberg, A., Degois, M., Guenot, L., and Athias, P.: Primary Rat Cardiac Cell Culture: Diet of the Mother Rats as a Determinant Parameter of Cardiomyoblast Production from Neonates , Biol Cell 57, 89, 1986
 
Cells:      Myoblast, cardiac
Enzyme(s)     Trypsin: 0.1%
Medium     Standardized Medium
(see reference)


Heart: Rat
  • Rat, SD, timid-pregnant
Freerksen, D., Schroedl, N., and Hartzell, C.: Control of Enzyme Activity Levels by Serum and Hydrocortisone in Neonatal Rat Heart Cells Cultured in Serum-Free Medium , J Cell Physiol 120, 126, 1984
 
Cells:      Myocytes
Enzyme(s)     Trypsin: 2.4 u/ml
Medium     DMEM


Heart: Rat
  • Rat, SD, female, 12 weeks
Piper, H.M., Probst, I., Schwartz, P., Hutter, F.J., and Spieckermann, P.G.: Culturing of Calcium Stable Adult Cardiac Myocytes , J Mol Cell Cardiol 14, 397, 1982
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 1: 0.06%
Medium     Krebs Ringer bicarbonate buffer


Heart: Rat
  • Rat, male/female, 200-250 g
Nag, A. and Cheng, M.: Adult Mammalian Cardiac Muscle Cells in Culture , Tissue Cell 13, 515, 1981
 
Cells:      Muscle
Enzyme(s)     Hyaluronidase: 0.1%
Medium     Krebs Ringer phosphate buffer, CMF


Heart: Rat
  • Rat, female, adult, 200 - 300 g
Claycomb, W.C., and Palazzo, M.C.: Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study , Dev Biol 80, 466, 1980
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     Joklik's MEM


Heart: Rat
  • Rat, SD, adult, male, 200-250 g
Frangakis, C., Bahl, J., McDaniel, H., and Bressler, R.: Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation , Life Sci 27, 815, 1980
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.10%
Medium     Joklik MEM, CF


Heart: Rat
  • Rat, SD, female, 12 wks, 250 g
Powell, T., Terrar, D.A., and Twist, V.W.: Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium , J Physiol 302, 131, 1980
 
Cells:      Myocytes
Ventricular myocardium
Enzyme(s)     Collagenase Type 1: 0.20%
Medium     Kreb's Ringer bicarbonate buffer


Heart: Rat
  • Rat, 10 wk
Bishop, S., Oparil, S., Reynolds, R., and Drummond, J.: Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats , Hypertension 1 (4), 378, 1979
 
Cells:      Myocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     Saline


Heart: Rat
  • Rat, male, 200-300 g
Farmer, B., Harris, R., Jolly, W., Hathaway, D., Katzberg, A., Watanabe, A., Whitlow, A., and Besch, H.: Isolation and Characterization of Adult Rat Heart Cells , Arch Biochem Biophys 179, 545, 1977
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 82 u/ml
Medium     Kreb's Henseleit buffer


Heart: Rat
  • Rat, female, albino, 200-250 g
Nag, A., Fischman, D., Aumont, M., and Zak, R.: Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability , Tissue Cell 9 (3), 419, 1977
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.1%
Medium     Kreb's Ringers phosphate


Heart: Rat
  • Rat, adult, male, 150-200g
Grosso, D., Frangakis, C., Carlson, E., and Bressler, R.: Isolation and Characterization of Myocytes From the Adult Rat Heart , Prep Biochem 7 (5), 383, 1977
 
Cells:      Myocytes
Enzyme(s)     Collagenase: 0.1%
Hyaluronidase: 0.1%
Medium     MEM CF


Heart: Rat
  • Rat, adult
Moustafa, E., Skomedal, T., Osnes, J., and Oye, I.: Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+ , Biochim Biophys Acta 421, 411, 1976
 
Cells:      Myocytes
Enzyme(s)     Collagenase: 0.1%
Medium     Perfusing solution


Heart: Rat
  • Rat, SD, albino, male, 180-230 g
Glick, M., Burns, A., and Reddy, W.: Dispersion and Isolation of Beating Cells from Adult Rat Heart , Anal Biochem 61, 32, 1974
 
Cells:      Heart ventricles, beating
Enzyme(s)     Collagenase: 0.05-0.2%
Medium     Phosphate buffer


Heart: Rat
  • Rat, Hebrew Un strain, 3 & 17 months, 150-210 g
Bierman, E.L., Stein, O., and Stein, Y.: Lipoprotein Uptake and Metabolism by Rat Aortic Smooth Muscle Cells in Tissue Culture , Circ Res 35, 136, 1974
 
Cells:      Myocytes
Enzyme(s)     Trypsin: 0.05%
Medium     Versene buffer


Heart: Rat
  • Rat, 2-5 day
Speicher, D., and McCarl, R.: Pancreatic Enzyme Requirements for the Dissociation of Rat Hearts for Culture , In Vitro 10, 30, 1974
 
Cells:      Myocytes
Enzyme(s)     Trypsin: 0.01%
Medium     Saline A


Heart: Rat
  • Rat, SD, male, 150-200 g
Pretlow II, T., Glick, M., and Reddy, W.: Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells , Am J Pathol 67 (2), 215, 1972
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.05%
Medium     (see reference)


Heart: Rat
  • Rat, albino, male, 250 g
Berry, M., Friend, D., and Scheuer, J.: Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart , Circ Res 26, 679, 1970
 
Cells:      Myocytes
Enzyme(s)     Hyaluronidase: 0.20%
Medium     Hank's solution, CF


Heart: Rat
  • Rat, adult, male, 250 g
Vahouny, G.V., Wei, R., Starkweather, R., and Davis, C.: Preparation of Beating Heart Cells From Adult Rats , Science 167, 1616, 1970
 
Cells:      Myocytes
Enzyme(s)     Trypsin: 0.1%
Medium     Saline A


Heart: Rat
  • Rat, Wistar, 1-4 day old
Mark, G., and Strasser, F.: Pacemaker Activity and Mitosis in Cultures of Newborn Rat Heart Ventricle Cells , Exp Cell Res 44, 217, 1966
 
Cells:      Myocytes
Enzyme(s)     Trypsin: 0.125%
Medium     Gey's BSS


Heart: Shellfish
  • Pacific Oysters
Le Duff, R., Lipart, C., and Renault, T.: Primary Culture of Pacific Oyster, Crassostrea gigas, heart cells, J Tiss Cul Meth 16, 67, 1994
 
Cells:      Haemocytes
Enzyme(s)     Trypsin:
Medium     L15 medium


Heart: Shellfish
  • Clams, 3.0 to 4.5 cm
Wen, C., Kou, G., and Chen, S.: Cultivation of Cells From the Heart of the Hard Clam, Merretrix lusoria (Roding), J Tiss Cul Meth 15, 123, 1993
 
Cells:      Myocytes
Fibroblasts
Enzyme(s)     Collagenase Type 1: 2%
Medium     L15 medium


Intestine: Canine
  • Dog, adult
Damholt AB, Buchan AM, Kofod H: Glucagon-like-peptide-1 secretion from canine L-cells is increased by glucose-dependent-insulinotropic peptide but unaffected by glucose , Endocrinology 139, 2085-91, 1998
 
Cells:      Intestinal L-cells
Enzyme(s)     Collagenase Type 1: 75 u/ml
Medium     HBSS


Intestine: Human
  • Human
Doyle, C., Vine, E, Bertram, K., Baharlou, H., Rhodes, J., Dervish, S., Gosselink, M., Di Re, A., Collins, G., Reza, F., Toh, J., Pathma-Nathan, N., Ahlenstiel, G., Ctercteko, G., Cunningham, A., Harm, A and Byrne, S.: Optimal Isolation Protocols for Examining and Interrogating Mononuclear Phagocytes From Human Intestinal Tissue. ,Front Immunol Vol. 12,, , 727952, 2021
 
Cells:     
Enzyme(s)     Collagenase Type 4: 0.3%
Deoxyribonuclease I: 5 ul/ml
Medium    


Intestine: Human
  • Human
Trapecar, M., Khan, S., Roan, N., Chen, T., Telwatte, S., Deswal, M., Pao, M., Somsouk, M., Deeks, S., Hunt, P., Yukl, S. and Sanjabi, S.: An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. , AIDS Res Hum Retroviruses 33, S31-S39, 2017
 
Cells:      Lymphocytes
Enzyme(s)     CLSPA: 0.05%
Deoxyribonuclease I: 0.00075%
Medium     RPMI-1640


Intestine: Human
  • Human
Kessler, S., Rho, H., West, G., Fiocchi, C., Drazba, J. and de la Motte, C.: Hyaluronan (HA) Deposition Precedes and Promotes Leukocyte Recruitment in Intestinal Inflammation. , Clin Transl Sci 1, 57-61, 2008
 
Cells:      Microvessel endothelial
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     HBSS


Intestine: Human
  • Human
Ebert, Ellen.: CD2 Activation of Human Lamina Propria Lymphocytes Reduces CD3 Responsiveness. , Immunology 117, 71-7, 2006
 
Cells:      Lamina propria lymphocytes
Enzyme(s)     Collagenase: 20 u/ml
Medium     Medium


Intestine: Human
  • Human
Kanai Takanori, Totsuka Teruji, Uraushihara Koji, Makita Shin, Nakamura Tetsuya, Koganei Kazutaka, Fukushima Tsuneo, Akiba Hisaya, Yagita Hideo, Okumura Ko, Machida Utako, Iwai Hideyuki, Azuma Miyuki, Chen Lieping, Watanabe Mamoru: Blockade of B7-H1 suppresses the development of chronic intestinal inflammation , J Immunol 171, 4156-63, 2003
 
Cells:      Lamina propria mononuclear cells
Enzyme(s)     Collagenase: 0.02%
Medium     HBSS


Intestine: Human
  • Human
Fahlgren, A., Hammarstrom, S., Danielsson, A. and Hammarstrom, M.: Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. , Clin Exp Immunol 131, 90, 2003
 
Cells:      Intestinal epithelial
Enzyme(s)     Collagenase Type 4: 72.5 u/ml
Medium     HBSS


Intestine: Human
  • Human
Stallmach A, Schaffer F, Hoffmann S, Weber S, Muller-Molaian I, Schneider T, Kohne G, Ecker KW, Feifel G, Zeitz M: Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis , Gut 43, 499-505, 1998
 
Cells:      Mucosal mononuclear cells
Enzyme(s)     Collagenase Type 3: 0.01%
Deoxyribonuclease I: 0.01%
Soybean Trypsin Inhibitor: 0.01%
Medium     RPMI


Intestine: Mouse
  • Mouse
Emgard, J., Kammoun, H., Garcia-Cassani, B., Chesne, J., Parigi, S., Jacob, J., Cheng, H, Evren, E., Das, S., Czarnewski, P., Sleiers, N. Melo-Gonzalez, F., Kvedaraite, E. Svensson, M. and Scandella, E.: Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. , Immunity 48, 120-132.e8, 2018
 
Cells:      Lamina propria lymphocytes
Enzyme(s)     Collagenase Type 4: 0.02%
Deoxyribonuclease I: 0.01%
Medium     HBSS


Intestine: Mouse
  • Mouse, embryonic
Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J and Ming L: Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase. , Braz J Med Biol Res 50, e5831, 2017
 
Cells:      Crypt
Enzyme(s)     Collagenase Type 1: 200 u/ml
Hyaluronidase: 100u/ml
Medium     DMEM


Intestine: Mouse
  • Mouse
O'Rourke, K., Ackerman, S., Dow, L. and Lowe, S.: Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. , Bio Protoc 6, , 2016
 
Cells:      Intestinal organoids
Enzyme(s)     Collagenase Type 4: 500 u/ml
Deoxyribonuclease I: 15 u/ml
Medium     Basal Medium


Intestine: Mouse
  • Mouse
Seo, S., Kuffa, P., Kitamoto, S., Nagao-Kitamoto, H., Rousseau, J., Kim, Y., Nunez, G. and Kamada, N.: Intestinal Macrophages Arising from CCR2(+) Monocytes Control Pathogen Infection by Activating Innate Lymphoid Cells. , Nat Commun 6, 8010, 2015
 
Cells:      Lamina propria mononuclear cells
Enzyme(s)     Collagenase Type 3: 400 u/ml
Deoxyribonuclease I: 0.001%
Medium     HBSS


Intestine: Mouse
  • Mouse
Beura, L., Anderson, K., Schenkel, J., Locquiao, J., Fraser, K., Vezys, V., Pepper, M. and Masopust, D.: Lymphocytic Choriomeningitis Virus Persistence Promotes Effector-Like Memory Differentiation and Enhances Mucosal T cell Distribution. , J Leukoc Biol 97, 217, 2015
 
Cells:      Lymphocytes
Enzyme(s)     Collagenase Type 1: 100 u/ml
Medium     RPMI-1640


Intestine: Mouse
  • Mouse, P4
Hotta, R., Stamp, L., Foong, J., McConnell, S., Bergner, A., Anderson, R., Enomoto, H., Newgreen, D., Obermayr, F., Furness, J. and Young, H.: Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. , J Clin Invest 123, 1182, 2013
 
Cells:      Enteric neural crest progenitors
Enzyme(s)     Neutral Protease: 0.5%
Collagenase animal free: 0.05%
Medium     DMEM/F12


Intestine: Mouse
  • Mouse
Xue, X. and Shah, Y.: In Vitro Organoid Culture of Primary Mouse Colon Tumors. , J Vis Exp , e50210, 2013
 
Cells:      Colon tumor organoids
Enzyme(s)     Collagenase Type 4: 200 u/ml
Neutral Protease: 0.01%
Medium     DMEM


Intestine: Mouse
Zhang, Y. and Hu, W.: Mouse Enteric Neuronal Cell Culture. , Methods Mol Biol 1078, 55-63, 2013
 
Cells:      Enteric neurons
Enzyme(s)     Collagenase Type 4: 0.1%
Trypsin: 0.05%
Deoxyribonuclease I: 400 u/ml
Medium     DMEM/F12


Intestine: Mouse
  • Mouse
Barthel, E., Speer, A., Levin, D., Sala, F., Hou, X., Torashima, Y., Wigfall, C. and Grikscheit, T.: Tissue Engineering of the Intestine in a Murine Model. , J Vis Exp 70, e4279, 2012
 
Cells:      Intestinal organoids
Enzyme(s)     Collagenase Type 1: 800 u/ml
Neutral Protease: 0.013%
Medium     DMEM


Intestine: Mouse
  • Mouse
Geem, D., Medina-Contreras, O., Kim, W., Huang, C. and Denning T.: Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine. , J Vis Exp 63, e4040, 2012
 
Cells:      Dendritic, macrophages
Enzyme(s)     Collagenase: 0.15%
Deoxyribonuclease I: 0.004%
Medium     HBSS


Intestine: Mouse
  • Mouse
Ruiz, V., Sachdev, M., Zhang, S., Wen, S. and Moss, S.: Isolating, Immunophenotyping and Ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes During Murine Helicobacter Pylori Infection. , J Immunol Methods 384, 157-63, 2012
 
Cells:      Lyphocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Neutral Protease: 0.1%
Deoxyribonuclease I: 200 ug/ml
Medium     RPMI 1640


Intestine: Mouse
  • Mouse, 2-3 month
Becker, L., Kulkarni, S., Tiwari, G., Micci, M. and Pasricha, P.: Divergent Fate and Origin of Neurosphere-Like Bodies from Different Layers of the Gut. , Am J Physiol Gastrointest Liver Physiol 302, G958-65, 2012
 
Cells:      Enteric neural stem cells
Enzyme(s)     Collagenase: 0.11%
Neutral Protease: 0.1%
Deoxyribonuclease I: 50 u/ml
Medium     M199


Intestine: Mouse
  • Mouse, neonatal and adult
Joseph, N., He, S., Quintana, E., Kim, Y., Nunez, G. and Morrison, S.: Enteric Glia are Multipotent in Culture but Primarily Form Glia in the Adult Rodent Gut. , J Clin Invest 121, 3398, 2011
 
Cells:      Myenteric plexus
Enzyme(s)     Collagenase Type 4: 0.025-0.1%
Papain: 10 u/ml
Deoxyribonuclease I: 100 u/ml
Medium     HBSS


Intestine: Mouse
  • Mouse
Ito, Y., Kanai, T., Totsuka, T., Okamoto, R., Tsuchiya, K., Nemoto, Y., Yoshioka, A., Tomita, T., Nagaishi, T., Sakamoto, N., Sakanishi, T., Okumura, K., Yagita, H. and Watanabe, M.: Blockade of NKG2D Signaling Prevents the Development of Murine CD4+ T Cell-Mediated Colitis. , Am J Physiol Gastrointest Liver Physiol 294, G199, 2008
 
Cells:      Lamina propria lymphocytes
Enzyme(s)     Collagenase Type 1: 0.3%
Deoxyribonuclease I: 0.01%
Medium     RPMI 1640


Intestine: Mouse
  • Mouse
Forbes, E., Groschwitz, K., Abonia, J.P., Brandt, E., Cohen, E., Blanchard, C., Ahrens, R., Seidu, L., McKenzie, A., Strait, R., Finkelman, F., Foster, P., Matthaei, K., Rothenberg, M. and Hogan, S.: IL-9- and Mast Cell-Mediated Intestinal Permeability Predisposes to Oral Antigen Hypersensitivity. , J Exp Med 205, 897, 2008
 
Cells:      Intestinal mononuclear
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     RPMI 1640


Intestine: Mouse
  • Mouse, Mastomys natalensis
Kidd, M., Modlin, I., Eick, G. and Champaneria, M.: Isolation, Functional Characterization, and Transcriptome of Mastomys Ileal Enterochromaffin Cells. , Am J Physiol Gastrointest Liver Physiol 291, G778-91, 2006
 
Cells:      Enterochromaffin cells
Enzyme(s)     Collagenase: 0.025%
Pronase E: 0.07%
Medium     HBSS


Intestine: Mouse
  • Mouse, 7 week
Goto Kazunori, Matsuoka Satoshi, Noma Akinori: Two types of spontaneous depolarizations in the interstitial cells freshly prepared from the murine small intestine , J Physiol 559, 411-22, 2004
 
Cells:      Interstitial cells
Enzyme(s)     Collagenase: 0.04%
Trypsin: 0.02%
Medium     see reference


Intestine: Mouse
  • Mouse, 9-13 day
Ordag Tamas, Redelman Doug, Miller Lisa J, Horvath Viktor J, Zhong Qiao, Almeida-Porada Graca, Zanjani Esmail D, Horowitz Burton, Sanders Kenton M: Purification of interstitial cells of Cajal by fluorescence-activated cell sorting , Am J Physiol Cell Physiol 286, C448-56, 2004
 
Cells:      Interstitial cells of Cajal
Enzyme(s)     Collagenase Type 2: 0.13%
Medium     HBSS


Intestine: Mouse
  • Mouse, 0-15 day
Lee Young Mee, Kim Byung Joo, Kim Hyun Jin, Yang Dong Ki, Zhu Mei Hong, Lee Kyu Pil, So Insuk, Kim Ki Whan: TRPC5 as a candidate for the nonselective cation channel activated by muscarinic stimulation in murine stomach , Am J Physiol/Gastro 284, G604-16, 2003
 
Cells:      Interstitial cells of Cajal
Enzyme(s)     Collagenase Type 2: 0.13%
Medium     Hanks


Intestine: Mouse
  • Mouse
Wu Y, Wang X, Csencsits KL, Haddad A, Walters N, Pascual DW: M cell-targeted DNA vaccination , Proc Natl Acad Sci U S A 98, 9318-23, 2001
 
Cells:      Lamina propria lymphocytes
Enzyme(s)     Collagenase Type 4: 300 u/ml
Medium     PBS


Intestine: Mouse
  • Mouse, F14
Sakagami Y, Inaguma Y, Sakakura T, Nishizuka Y: Intestine-like remodeling of adult mouse glandular stomach by implanting of fetal intestinal mesenchyme , Cancer Res 44, 5845-9, 1984
 
Cells:      Intestinal mesenchymal
Enzyme(s)     Collagenase Type 2: 0.03%
Medium     HBSS


Intestine: Rat
  • Rat, postnatal, adult
Kruger, G., Mosher, J., Bixby, S., Joseph, N., Iwashita, T. and Morrison, S.: Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness. , Neuron 35, 657, 2002
 
Cells:      Gut neural crest stem
Enzyme(s)     Collagenase Type 4: 0.1%
Trypsin: 0.025%
Medium     HBSS


Intestine: Rat
  • Rat, SD, embryonic
Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. , Neuron 35, 643, 2002
 
Cells:      Sciatic nerve and gut neural crest stem
Enzyme(s)     Collagenase Type 4: 0.025%
Trypsin: 0.005%
Deoxyribonuclease I: 0.05%
Medium     HBSS


Intestine: Rat
  • Rat, male, 18-24 day
Quaroni A, Wands J, Trelstad RL, Isselbacher KJ: Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria , J Cell Biol 80, 248-65, 1979
 
Cells:      Intestinal epithelial
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Kidney: Avian
  • Avian, house sparrows
Goldstein D., Reddy V., and Plaga K.: Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones , Am J Physiol 276, R847, 1999
 
Cells:      Tubule segments
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     HBSS


Kidney: Avian
  • Avian, chicken
Reich, C. and Bonar, R.: Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture, Meth Cell Sci 7, 97, 1982
 
Cells:      Tubule and glomeruli
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     RPMI 1640


Kidney: Bovine
  • Bovine
Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255, F1160, 1988
 
Cells:      Papillary duct
Enzyme(s)     Hyaluronidase: 0.2%
Medium     Keri's buffer
HEPES buffered saline


Kidney: Canine
  • Canine
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10, 171, 2011
 
Cells:      Renal adipose derived cells
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     DMEM


Kidney: Canine
  • Dog, 1-5 months
States, B., Reynolds, R., Lee, J., and Segal, S.: Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments , In Vitro Cell Dev Biol 26, 105, 1990
 
Cells:      Proximal tubules
Enzyme(s)     Collagenase Type 1: 0.15%
Medium     Krebs Ringer bicarbonate buffer


Kidney: Canine
  • Dog, mongrel, 20 Kg
Yau, C., Rao, L., and Silverman, M.: Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells , Can J Physiol Pharmacol 63, 417, 1985
 
Cells:      Proximal tubular
Enzyme(s)     Deoxyribonuclease I: 0.0125%
Medium     (see reference)


Kidney: Fish
  • Winter flounder (Pseudopleur-onectes americanus)
    200 - 500 g
Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport , Am J Physiol 251, 424, 1986
 
Cells:      Renal tubule
Enzyme(s)     Trypsin: 0.2%
Trypsin: 0.2%
Medium     CMF solution


Kidney: Guinea-Pig
  • Guinea-pig, male, 100 g
Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase , In Vitro 8, 101, 1972
 
Cells:      Single cells
Enzyme(s)     Trypsin: 0.25%
Medium     CF salt solution


Kidney: Hamster
  • Hamster
    (also rat, SD and Wistar, 150-225 g, rabbit, bovine)
Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255, F1160, 1988
 
Cells:      Papillary duct
Enzyme(s)     Hyaluronidase: 0.2%
Medium     Keri's buffer
HEPES buffered saline


Kidney: Human
  • Human
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10, 171, 2011
 
Cells:      Renal adipose derived cells
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     DMEM


Kidney: Human
  • Human, adult
Valente, M., Henrique, R., Costa, V., Jeronimo, C., Carvalho, F., Bastos, M., Guedes de Pinho, P. and Carvalho, M.: A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens. , PLoS ONE 6, e19337, 2011
 
Cells:      Renal tumor cells and proximal tubular epithelial
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM/F12


Kidney: Human
  • Human
Presnell, S., Bruce, A., Wallace, S., Choudhury, S., Genheimer, C., Cox, B., Guthrie, K., Werdin, E., Tatsumi-Ficht, P., Ilagan, R., Kelley, R., Rivera, E., Ludlow, J., Wagner, B., Jayo, M. and Bertram, T.: Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys. , Tiss Eng 17, 261, 2011
 
Cells:      Renal cells
Enzyme(s)     Collagenase Type 4: 300 u/ml
Neutral Protease: see reference
Medium     HBSS


Kidney: Human
  • Human, adult
Johnson, D., Saunders, H., Johnson, F., Huq, S., Field, M., and Pollock, C.: Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor , J Pharmacol Exp Ther 289, 535-42, 1999
 
Cells:      Renal proximal tubule and cortical fibroblasts
Enzyme(s)     Collagenase Type 2: 383 u/ml
Medium     DMEM/F-12


Kidney: Human
  • Human
McAteer, J, Kempson, S., and Evan, A: Culture of Human Renal Cortex Epithelial Cells, J Tiss Cul Meth 13, 143, 1991
 
Cells:      Renal cortex
Enzyme(s)     Trypsin: 0.1%
Medium     Tissue Culture Grade Water


Kidney: Human
  • Human
Trifillis, A. and Kahng, M.: Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells , In Vitro Cell Dev Biol 26, 441, 1990
 
Cells:      Papillary duct
Enzyme(s)     Collagenase: 400 u/ml
Medium     Eagle's MEM-HEPES buffer w/L-glutamine


Kidney: Human
  • Human
Heieren, M., van der Woude, F., and Balfour Jr., H.: Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells , Proc Natl Acad Sci U S A 85, 1642, 1988
 
Cells:      Mesangial
Enzyme(s)     Trypsin: 0.25%
Medium     DMEM/Ham's F-12


Kidney: Human
  • Human, adult
Yang, A., Gould-Kostka, J., and Oberley, T.: In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate , In Vitro Cell Dev Biol 23 (1), 34, 1987
 
Cells:      Tubular
Enzyme(s)     Collagenase: 250 u/ml
Medium     PBS


Kidney: Human
  • Human, adult, 14-66 years
Trifillis, A., Regec, A., and Trump, B.: Isolation, Culture, and Charaterization of Human Renal Tubular Cells , J Urol 133, 324, 1985
 
Cells:      Tubular
Enzyme(s)     Collagenase: 100 u/ml
Medium     Joklik's MEM


Kidney: Human
  • Human
Hemstreet, G., Enoch, P., and Pretlow, T.: Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification , Cancer Res 40, 1043, 1980
 
Cells:      Malignant
Stromal
Enzyme(s)     Papain: 0.009%
Medium     Sacks solution


Kidney: Human
  • Human, 24 newborn and stillborn
    (also rabbit)
De Oca, H., and Malinin, T.: Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys , J Clin Microbiol 2, 243, 1975
 
Cells:      Renal
Enzyme(s)     Trypsin: 0.25%
Medium     See reference


Kidney: Monkey
  • Monkey
Melnick, J., and Wallis, C.: Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells , Dev Biol 37, 77, 1976
 
Cells:      Kidney
Enzyme(s)     Trypsin: 0.01% - 0.00001%
Medium     Eagle's MEM


Kidney: Mouse
  • Mouse
Yuan, Y., Li, L., Zhu, L., Liu, F., Tang, X., Liao, G., Liu, J., Cheng, J., Chen, Y. and Lu, Y.: Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy. , Stem Cells 38, 639-652, 2020
 
Cells:      Mactrophages
Enzyme(s)     Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.01%
Medium     DMEM


Kidney: Mouse
  • Mouse
Adam, M., Potter, A. and Potter, S.: Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development. , Development 144, 3625-3632, 2017
 
Cells:      Kidney
Enzyme(s)     Deoxyribonuclease I: 125 u/ml
Protease, Bacillus Licheniformis: 1%
Medium     DPBS


Kidney: Mouse
  • Mouse
DiRocco, D., Kobayashi, A., Taketo, M., McMahon, A. and Humphreys, B.: Wnt4/β-catenin Signaling in Medullary Kidney Myofibroblasts. , J Am Soc Nephrol 24, 1399, 2013
 
Cells:      Collecting duct epithelial
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DMEM


Kidney: Mouse
  • Mouse
Kabgani, N., Grigoleit, T., Schulte, K., Sechi, A., Sauer-Lehnen, S., Tag, C., Boor, P., Kuppe, C., Warsow, G., Schordan, S., Mostertz, J., Chilukoti, R., Homuth, G., Endlich, N., Tacke, F. and Weiskirchen, R.: Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin. , PLoS ONE 7, e34907, 2012
 
Cells:      Glomeruli
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     RPMI 1640


Kidney: Mouse
  • Mouse
Sedrakyan, S., Da Sacco, S., Milanesi, A., Shiri, L., Petrosyan, A., Varimezova, R., Warburton, D., Lemley, K., De Filippo, R. and Perin, L.: Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis. , J Am Soc Nephrol 23, 661-73, 2012
 
Cells:      Glomeruli
Enzyme(s)     Collagenase Type 1: 0.1%
Deoxyribonuclease I: 100 u/ml
Medium     DMEM


Kidney: Mouse
  • Mouse, 10-14 week
Park, J., Pasupulati, R., Feldkamp, T., Roeser, N. and Weinberg, J.: Cyclophilin D and the Mitochondrial Permeability Transition in Kidney Proximal Tubules after Hypoxic and Ischemic Injury. , Am J Physiol/Renal 301, F134, 2011
 
Cells:      Proximal tubule
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     See reference


Kidney: Mouse
  • Mouse, embryonic
Brown, A., Blank, U., Adams, D., Karolak, M., Fetting, J., Hill, B. and Oxburgh, L.: Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney. , J Vis Exp 50, e2555, 2011
 
Cells:      Kidney
Enzyme(s)     Collagenase Type 1: 0.25%
Pancreatin: 1.0%
Deoxyribonuclease I: 1 u/ml
Medium     HBSS


Kidney: Mouse
  • Mouse, adult
Huang, Y., Johnston, P., Zhang, B., Zakari, A., Chowdhry, T., Smith, R., Marban, E., Rabb, H. and Womer, K.: Kidney-Derived Stromal Cells Modulate Dendritic and T Cell Responses. , J Am Soc Nephrol 20, 831-41, 2009
 
Cells:      Kidney sphere-derived
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     Iscove's Modified Dulbecco's


Kidney: Mouse
  • Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. , Oxid Med Cell Longev 1, 33, 2008
 
Cells:      Renal tubular epithelial
Enzyme(s)     Collagenase: 200 u/ml
Soybean Trypsin Inhibitor: see reference
Medium     HBSS


Kidney: Mouse
  • Mouse
Wright, J., Morales, M., Sousa-Menzes, J., Ornellas, D., Sipes, J., Cui, Y., Cui, I., Hulamm, P., Cebotaru, V., Cebotaru, L., Guggino, W. and Guggino, S.: Transcriptional Adaptation to Clcn5 Knockout in Proximal Tubules of Mouse Kidney. , Physiol Genomics 33, 341, 2008
 
Cells:      Proximal tubule
Enzyme(s)     Collagenase Type 2: 0.013%
Medium     see reference


Kidney: Mouse
  • Mouse, 4 week
Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis. , Am J Physiol Cell Physiol 292, C2070, 2007
 
Cells:      Endothelial kidney
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     DMEM


Kidney: Mouse
  • Mouse, adult
Syal Ashu, Schiavi Susan, Chakravarty Sumana, Dwarakanath Vangipuram, Quigley Raymond, Baum Michel: Fibroblast growth factor-23 increases mouse PGE2 production in vivo and in vitro , Am J Physiol/Renal 290, F450-5, 2006
 
Cells:      Cortex, proximal tubule
Enzyme(s)     Collagenase: 0.15%
Medium     DMEM


Kidney: Mouse
  • Mouse
Plotkin, M. and Goligorsky, M.: Mesenchymal Cells from Adult Kidney Support Angiogenesis and Differentiate into Multiple Interstitial Cell Types including Erythropoietin-Producing Fibroblasts. , Am J Physiol Renal Physiol 291, F902-12, 2006
 
Cells:      Kidney mesenchymal
Enzyme(s)     Collagenase: 250 u/ml
Neutral Protease: 33.3 u/ml
Medium     DMEM/F12


Kidney: Mouse
  • Mouse, 10-12 week
Dekel, B., Zangi, L., Shezen, E., Reich-Zeliger, S., Eventov-Friedman, S., Katchman, H., Jacob-Hirsch, J., Amariglio, N., Rechavi, G., Margalit, R. and Reisner, Y.: Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney. , J Am Soc Nephrol 17, 3300, 2006
 
Cells:      Kidney
Enzyme(s)     Collagenase Type 4: 0.075%
Medium     DMEM


Kidney: Mouse
  • Mouse, male, 12-16 week
Cunningham, R., Xiaofei, E., Steplock, D., Shenolikar, S. and Weinman, E.: Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media. , Am J Physiol Renal Physiol 289, F933, 2005
 
Cells:      Renal proximal tubule cells
Enzyme(s)     Collagenase Type 2: 0.1%
Soybean Trypsin Inhibitor: 0.25%
Medium     DMEM/F-12


Kidney: Mouse
  • Mouse, male, 2 month
Sindic, A., Velic, A., Basoglu, C., Hirsch, J., Edemir, B., Kuhn, M. and Schlatter, E.: Uroguanylin and Guanylin Regulate Transport of Mouse Cortical Collecting Duct Independent of Guanylate Cyclase C. , Kidney Int 68, 1008, 2005
 
Cells:      Cortical collecting duct
Enzyme(s)     Collagenase: 54-178 u/ml
Protease: see reference
Medium     MEM


Kidney: Mouse
  • Mouse, 6 week
Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE.: Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis , J Biol Chem 277(30), 27535, 2002
 
Cells:      Glomerular mesangial cells
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     RPMI 1640


Kidney: Mouse
  • Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation , Mol Biol Cell 10, 1569-79, 1999
 
Cells:      Fibroblasts, mesangial, smooth muscle
Enzyme(s)     Trypsin: 0.25%
Collagenase: see reference
Soybean Trypsin Inhibitor: 0.05%
Medium     DMEM


Kidney: Mouse
  • Mouse
Taub, M: Renal Tubule Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley & Sons, Ltd., 14B:6.1, 1995
 
Cells:      Epithelial
Enzyme(s)     Collagenase Type 4: 1%
Medium     DMEM /F-12


Kidney: Mouse
  • Mouse, naive SJL (H-2S)
Haverty, T., Kelly, C., Hines, W., Amenta, P., Watanabe, M., Harper, R., Kefalides, N., and Neilson, E.: Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis , J Cell Biol 107, 1359, 1988
 
Cells:      Proximal tubular epithelial
Enzyme(s)     Deoxyribonuclease I: 15 µg/ml
Medium     RPMI 1640


Kidney: Porcine
  • Porcine, hybrid
Heussner, A. and Dietrich, D.: Primary Porcine Proximal Tubular Cells as an Alternative to Human Primary Renal Cells in Vitro: an Initial Characterization. , BMC Cell Biol 14, 55, 2013
 
Cells:      Kidney
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Kidney: Rabbit
  • Rabbit
Nowak, G. and Bakajsova, D.: Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes. , J Vis Exp 71, e4301, 2013
 
Cells:      Renal proximal tubular
Enzyme(s)     Collagenase Type 1: 60 u/ml
Medium     DMEM/F12


Kidney: Rabbit
  • Rabbit, New Zealand
Taub, M.: Primary Kidney Proximal Tubule Cells. , Methods Mol Biol 290, 231, 2005
 
Cells:      Proximal tubule cells
Enzyme(s)     Collagenase Type 4: 0.005%
Soybean Trypsin Inhibitor: 0.00025%
Medium     DMEM/F-12


Kidney: Rabbit
  • Rabbit, New Zealand White, female
Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments , Am J Physiol 273, F650, 1997
 
Cells:      Nephron segments
Enzyme(s)     Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
Medium     Eagle's MEM


Kidney: Rabbit
  • Rabbit, New Zealand white, male, 1.5 - 2.0 Kg
Naray-Fejes-Toth, A., and Fejes-Toth, G.: Immunoelection and Culture of Cortical Collecting Duct Cells, J Tiss Cul Meth 13, 179, 1991
 
Cells:      Duct
Enzyme(s)     Soybean Trypsin Inhibitor: 0.025%
Medium     Hank's Solution with calcium and HEPES


Kidney: Rabbit
  • Rabbit, New Zealand white, female, 2-3 Kg
Rodeheaver, D., Aleo, M., and Schnellmann, R.: Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation , In Vitro Cell Dev Biol 26, 898, 1990
 
Cells:      Renal proximal tubules
Enzyme(s)     Deoxyribonuclease I: 70 u/ml
Medium     Modified DME-F12


Kidney: Rabbit
  • Rabbit, New Zealand, white, 2-3 kg
Allen, M., Nakao, A., Sonnenburg, W., Burnatowska-Hledin, M., Speilman, W., and Smith, W.: Immunodissection of Cortical and Medullary Thick Ascending Limb Cells From Rabbit Kidney , Am J Physiol 255, F704, 1988
 
Cells:      MTALH cells
RCCT cells
Enzyme(s)     Collagenase: 0.1%
Medium     DMEM


Kidney: Rabbit
  • Rabbit
    (also rat, SD and Wistar, 150-225 g, hamster, bovine)
Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture , Am J Physiol 255, F1160, 1988
 
Cells:      Papillary duct
Enzyme(s)     Hyaluronidase: 0.2%
Medium     Keri's buffer
HEPES buffered saline


Kidney: Rabbit
  • Rabbit, New Zealand, white
Grenier, F., and Smith, W.: Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae, Prostaglandins 16, 759, 1978
 
Cells:      Collecting tubule
Enzyme(s)     Trypsin: 0.05%
Medium     Kreb's Ringer buffer


Kidney: Rabbit
  • Rabbit, New Zealand, white, female, 2.5 Kg
Dworzack, D., and Grantham, J.: Preparation of Renal Papillary Collecting Duct Cells for Study , Nat Neurosci 8, 191, 1975
 
Cells:      Papillary collecting duct
Enzyme(s)     Collagenase: 0.2%
Medium     (see reference)


Kidney: Rat
  • Rat, SD, fetal
Gupta, A., Jadhav, S., Tripathy, N. and Nityanand, S.: Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects. , PLoS ONE 10, e0131057, 2015
 
Cells:      Fetal kidney
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     MEM


Kidney: Rat
  • Rat, Lewis, male
Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney. , Lipids Health Dis 10, 171, 2011
 
Cells:      Renal adipose derived cells
Enzyme(s)     Collagenase Type 1: 0.3%
Medium     DMEM


Kidney: Rat
  • Rat
Panico, C., Luo, Z., Damiano, S., Artigiano, F., Gill, P. and Welch, W.: Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3. , Hypertension 54, 1291, 2009
 
Cells:      Proximal tubule
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     see reference


Kidney: Rat
  • Rat, SD, male, 200-250g
Silva, G., Beierwaltes, W., and Garvin, J.: Extracellular ATP stimulates NO production in rat thick ascending limb , Hypertension 47, 563-7, 2006
 
Cells:      Medullary thick ascending limb
Enzyme(s)     Collagenase: 0.1%
Medium     HEPES-saline


Kidney: Rat
  • Rat, Wistar, 300g
Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C: Kidney oxygen consumption, carbonic anhydrase, and proton secretion , Am J Physiol/Renal 290, F1009-15, 2006
 
Cells:      Renal proximal tubules
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM/F12


Kidney: Rat
  • Rat, Wistar, male, 300-350 g
Deng, A., Miracle, C., Suarez, J., Lortie, M., Satriano, J., Thomson, S., Munger, K. and Blantz, R.: Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II. , Kidney Int 68, 723, 2005
 
Cells:      Renal proximal tubules
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     DMEM/F-12


Kidney: Rat
  • Rat
Arystarkhova, E., Wetzel R., and Sweadner K.: Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney , Am J Physiol/Renal 282(3), F393, 2002
 
Cells:      Cortical tubule cells
Enzyme(s)     Collagenase: 0.6%
Medium     DMEM


Kidney: Rat
  • Rat, SD, male 275-300 g
Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B: Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose , Kidney Int Suppl 61, 1605-16, 2002
 
Cells:      Glomerular mesangial cells
Enzyme(s)     Collagenase Type 1: 250 u/ml
Medium     DMEM


Kidney: Rat
  • Rat, SD, 1 week
Valencia L., Bidet M., Martial S., Sanchez E., Melendez E., Tauc M., Poujeol C., Martin D., Namorado M., Reyes J., and Poujeol P.: Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture , Am J Physiol Cell Physiol 280(5), C1193, 2001
 
Cells:      Cortical collection duct
Enzyme(s)     Collagenase Type 2: 0.15%
Medium     DMEM/Ham's F12


Kidney: Rat
  • Rat, SD, 250-300 g
Li, N., Teggatz, E., Li, P., Allaire, R., and Zou, A.: Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels , Microvasc Res 60, 149, 2000
 
Cells:      Microvessels
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     PSS


Kidney: Rat
  • Rat, SD, 250-300 g
Mattson D., and Wu F.: Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature , Hypertension 35, 337, 2000
 
Cells:      Renal
Enzyme(s)     Collagenase Type 2: 0.2%
Medium     see reference


Kidney: Rat
  • Rat, SD, male, 5-6 week
Miyata, N., Park, F., Li, X., and Cowley, A.: Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney , Am J Physiol 277, F437, 1999
 
Cells:      Renal tubules
Enzyme(s)     Collagenase: 0.1%
Medium     see reference


Kidney: Rat
  • Rat, SD, 225-250 g
Amiri F, Garcia R: Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells , Am J Physiol 276, F691-9, 1999
 
Cells:      Glomerular mesangial cells
Enzyme(s)     Collagenase Type 1: 250 u/ml
Medium     DMEM


Kidney: Rat
  • Rat, SD, male
Eitle E, Hiranyachattada S, Wang H, Harris PJ: Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney , Am J Physiol 274, C1075-80, 1998
 
Cells:      Proximal tubule suspensions
Enzyme(s)     Collagenase Type 4: 0.1%
Pronase E: 2 u/ml
Medium     HEPES buffer


Kidney: Rat
  • Rat, SD, male
Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments , Am J Physiol 273, F650, 1997
 
Cells:      Nephron segments
Enzyme(s)     Collagenase Type 2: 0.05%
Deoxyribonuclease I: 50 u/ml
Medium     Eagle's MEM


Kidney: Rat
  • Rat, 12-weeks-old
Ishikawa, S., Kusaka, I., Higashiyama, M., Nagasaka, S., Saito, T., Honda, K., and Saito, T.: Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein , Nat Neurosci 50, 1506, 1996
 
Cells:      Renal
Enzyme(s)     Collagenase: 0.1%
Medium     DMEM


Kidney: Rat
  • Rat, male, 150-200 g
Elliget, K., and Trump, B.: Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury , In Vitro Cell Dev Biol 27, 739, 1991
 
Cells:      Epithelial
Proximal tubule
Enzyme(s)     Protease: 0.1%
Medium     HBSS/CMF


Kidney: Rat
  • Rat, adult
Brion, L., Schwartz, J., Lachman, H., Zavilowitz, B., and Schwartz, G.: Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells , Am J Physiol 257, F486, 1989
 
Cells:      Inner medullary collecting duct
Papillae
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     PBS


Kidney: Rat
  • Rat, Wistar, female, 100-150 g
Wang, J., Kester, M., and Dunn, M.: The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells , Biochim Biophys Acta 969, 217, 1988
 
Cells:      Glomerular mesangial
Enzyme(s)     Collagenase: 0.025%
Medium     RPMI 1640


Kidney: Rat
  • Rat, Wistar, male, 150 - 200 g
Barlet-Bas, C., Khadouri, C., Marsey, S., and Doucet, A.: Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine , Proc Natl Acad Sci U S A 85, 1707, 1988
 
Cells:      Renal target
Enzyme(s)     Collagenase: 1.0% (also 0.7%)
Medium     Eagle's MEM


Kidney: Rat
  • Rat, Wistar, male, adult
Gesek, F., Wolff, D., and Strandhov, J.: Improved Separation Method for Rat Proximal and Distal Renal Tubules , Am J Physiol 253, F358, 1987
 
Cells:      Proximal tubules
Enzyme(s)     Collagenase: 0.2%
Medium     Krebs-Henseleit buffer


Kidney: Rat
  • Rat,Wistar, 300-400 gm
Vinay, P., Gougoux, A., and Lemieux, G.: Isolation of a Pure Suspension of Rat Proximal Tubules , Am J Physiol 241, F403, 1981
 
Cells:      Proximal tubules
Enzyme(s)     Collagenase: 0.15 %
Medium     Krebs Henseleit solution


Kidney: Rat
  • Rat
Wallach, D., Anderson, W., and Pastan, I.: Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin , J Biol Chem 253, 24, 1978
 
Cells:      Fibroblasts
Kidney
Enzyme(s)     Trypsin: up to 0.25%
Medium     Dulbecco-Vogt MEM


Liver: Avian
  • Avian, various
Else, P., Brand, M., Turner, N. and Hulbert, A: Respiration Rate of Hepatocytes Varies With Body Mass in Birds , J Exp Biol 207, 2305, 2004
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     see reference


Liver: Avian
  • Ducklings, 7 day
Lee J., Culvenor J., Angus P., Smallwood R., Nicoll A., and Locarnini S.: Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells , J Virol 75(16), 7651, 2001
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Hyaluronidase: 0.05%
Medium     DMEM/ F12


Liver: Canine
  • Dog, 13 years old
    (also rat, guinea-pig, rabbit, human)
Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 90 u/ml
Medium     CF EGTA perfusate


Liver: Canine
  • Dog, adult
Vickrey, H., Ramon, J., and McCann, D.: Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions , In Vitro 15, 120, 1979
 
Cells:      Hepatocytes
Enzyme(s)     Trypsin: 0.1%
Medium     HBSS, CMF


Liver: Chicken
  • Chicken, adult
Fraslin, J., Touquette, L., Douaire, M., Menezo, Y., Guillemot, J., and Mallard, J.: Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture , In Vitro Cell Dev Biol 28, 615, 1992
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.02%
Medium     HEPES, CF


Liver: Chicken
  • Chicken
    (also rat, SD, 150-250 g)
Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars , J Biol Chem 254 (21), 10830, 1979
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 6000 units
Collagenase Type 3 & 4:
Medium     HEPES


Liver: Chicken
  • Chicken, Leghorn, white, 10-15 day
Tarlow, D., Watkins, P., Reed, R., Miller, R., Zwergel, E., and Lane, M.: Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture , J Cell Biol 73, 332, 1974
 
Cells:      Hepatocytes
Enzyme(s)     Deoxyribonuclease I: 0.00125%
Medium     PBS


Liver: Chicken
  • Chick embryos, 5 day
Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation , Exp Cell Res 30, 257, 1963
 
Cells:      Heart
Liver
Enzyme(s)     Trypsin: 3.0%
Medium     Tyrode's solution, CMF


Liver: Chicken
  • Chick embryonic
Rinaldini, L.: An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells , Exp Cell Res 16, 477, 1959
 
Cells:      Various tissues (heart, liver, skeletal, cardiac)
Enzyme(s)     Trypsin: various grades
Medium     CMF Tyrode's solution


Liver: Equine
  • Equine, 4-13 yr
Bakala A., Karlik W., and Wiechetek M.: Preparation of Equine Isolated Hepatocytes , Toxicol In Vitro 17(5-6), 615, 2003
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.1%
Medium     HBSS


Liver: Fish
  • Rainbow trout (Oncorhynchus mykiss), male & female,
    120-600 g
Blair, J.B., Miller, M.R., Pack, D., Barnes, R., Teh, S.J. and Hinton, D.E.: Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions , In Vitro Cell Dev Biol 26, 237, 1990
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.045%
Medium     Kreb's-Ringer bicarbonate buffer, CF


Liver: Fish
  • Trout, male, 100 - 200 g
Lipsky, M., Sheridan, T., Bennett, R., and May, E.: Comparison of Trout Hepatocyte Culture on Different Substrates, In Vitro Cell Dev Biol 22, 360, 1986
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.045%
Medium     HBSS with 0.05M HEPES


Liver: Fish
  • Rainbow trout, (Salmo gairdneri), male, 150-200 g
Klaunig, J., Ruch, R., and Goldblah, P.: Trout Hepatocyte Culture: Isolation and Primary Culture , In Vitro Cell Dev Biol 21, 221, 1985
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 100 u/ml
Medium     HBSS/CMF


Liver: Fish
  • Trout 100g - 5kg
Bailey, G., Taylor, M., and Selivonchick, D.: Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner , Carcinogenesis 3, 511, 1982
 
Cells:      Hepatocytes
Enzyme(s)     Hyaluronidase: 0.08%
Medium     See reference


Liver: Fish
  • Trout (Salmo gairdneri), male, 150-200 g
    Catfish (Ictalurus punctatus), male, 100 - 150 g
Klaunig, J.: Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies, Natl Cancer Inst Monogr 65, 163, 1981
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 100u/ml
Medium     HBSS


Liver: Guinea-Pig
  • Guinea-pig, Hartley, male, 250-300 g
Arinze, I. and Kawai, Y.: Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis , Arch Biochem Biophys 225, 196, 1983
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2:
Medium     Kreb's Ringer bicarbonate buffer


Liver: Guinea-Pig
  • Guinea-pig, young
Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 90 u/ml
Medium     CF EGTA perfusate


Liver: Human
  • Human
Reuschel, E, Jilg, W., Seelbach-Goebel, B. and Demi, L.: Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes , PLoS ONE , , 2019
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     HBSS


Liver: Human
  • Human
Werner, M., Driftmann, S., Kleinehr, K., Kaiser, G., Mathe, Z., Treckmann, J., Paul, A., Skibbe, K., Timm, J., Canbay, A., Gerken, G., Schlaak, J. and Broering, R.: All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells. , PLoS ONE 10, e0138655, 2015
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.06%
Medium     DMEM


Liver: Human
  • Human
Pfeiffer, E., Kegel, V., Zeilinger, K., Hengstler, J., Nussler, A., Seehofer, D. and Damm, G.: Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells. , Exp Biol Med (Maywood) 240, 645-56, 2015
 
Cells:      Hepatocytes and non-parenchymal cells
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Liver: Human
  • Human
Lee, S., Schelcher, C., Demmel, M., Hauner, M. and Thasler, W.: Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure. , J Vis Exp , , 2013
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.1-0.15%
Medium     HEPES


Liver: Human
  • Human, fetal
Vatakis, D., Bristol, G., Kim, S., Levin, B., Liu, W., Radu, C., Kitchen, S. and Zack, J.: Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. , J Vis Exp 70, e4181, 2012
 
Cells:      Liver hematopoietic
Enzyme(s)     Collagenase Type 4: 0.1%
Hyaluronidase: 0.1%
Deoxyribonuclease I: 2 u/ml
Medium     RPMI


Liver: Human
  • Human
Torok, E., Lutgehetmann, M., Bierwolf, J., Melbeck, S., Dullmann, J., Nashan, B., Ma, P. and Pollok, J.: Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering. , Liver Transpl 17, 104, 2011
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     Williams E


Liver: Human
  • Human
Wauthier, E., Schmelzer, E., Turner, W., Zhang, L., LeCluyse, E., Ruiz, J., Turner, R., Furth, M., Kubota, H., Lozoya, O., Barbier, C., McClelland, R., Yao, H., Moss, N., Bruce, A., Ludlow, J. and Reid, L.: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance. , Methods Cell Biol 86, 137, 2008
 
Cells:      Hepatic stem cells and heptoblasts
Enzyme(s)     Collagenase Type 4: 0.014-0.06%
Medium     various


Liver: Human
  • Human
Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC, and Maurel P: Human hepatocyte culture , Methods Mol Biol 320, 283, 2006
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     HEPES


Liver: Human
  • Human
Hughes, R., Mitry, R., Dhawan, A., Lehec, S., Girlanda, R., Rela, M., Heaton, N., and Muiesan, P.: Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation , Liver Transpl 12, 713, 2006
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     EBSS


Liver: Human
  • Human
Hussain, S., Strom, S., Kirby, M., Burns, S., Langemeijer, S., Ueda, T., Hsieh, M. and Tisdale, J.: Side Population Cells Derived from Adult Human Liver Generate Hepatocyte-Like Cells In Vitro. , Dig Dis Sci 50, 1755, 2005
 
Cells:      Hepatic side population
Enzyme(s)     Collagenase: 0.02-0.05%
Medium     HBSS


Liver: Human
  • Human, adult
Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R, and Gupta S.:   Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications , Liver Int 24, 361, 2004
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.6%
Medium     RPMI 1640


Liver: Human
  • Human
Duanmu Z., Locke D., Smigelski J., Wu W., Dahn M., Falany C., Kocarek T., and Runge-Morris M.: Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes , Drug Metab Dispos 30(9), 997, 2002
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Medium     Williams E


Liver: Human
  • Human, fetal
Malhi, H., Irani, A., Gagandeep, S. and Gupta, S.: Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes. , J Cell Sci 115, 2679, 2002
 
Cells:      Epithelial progenitor
Enzyme(s)     Collagenase: 0.03%
Medium     DMEM


Liver: Human
  • Human
    (also rat)
Dandri, M., Burda, M., Torok, E., Pollok, J., Iwanska, A., Sommer, G., et al.: Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus , Hepatol 33, 981, 2001
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     Leffert's buffer


Liver: Human
  • Human
Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers , Drug Metab Dispos 28, 1321-6, 2000
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     Williams E


Liver: Human
  • Human
Parzefall, W., Erber, E., Sedivy, R., and Schulte-Hermann, R.: Testing for Induction of DNA Synthesis in Human Hepatocyte Primary Cultures by Rat Liver Tumor Promoters , Cancer Res 51, 1143, 1991
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.025%
Medium     Williams E


Liver: Human
  • Human
Gomez-Lechon, M., Lopez, P., Donato, T., Montoya, A., Larrauri, A., Gimenez, P., Trullenque, R., Fabra, R., and Castell, J.: Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo , In Vitro Cell Dev Biol 26, 67, 1990
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     HEPES buffer
(see reference)


Liver: Human
  • Human, male, 17-40 yrs.
Begue, J., Baffet, G., Campion, J., and Guillouzo, A.: Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3 , Biol Cell 63, 327, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     HEPES buffer


Liver: Human
  • Human, 4 male kidney donors, age between 17 and 31
    (also adult rats, male, SD, 180-200 g)
LeBot, M., Begue, J., Kernaleguen, D., Robert, J., Ratanasavanh, D., Airiau, J., Riche, C., and Guillouzo, A.: Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes , Biochem Pharmacol 37 (20), 3877, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05% & 0.025%
Medium     HEPES
(see reference)


Liver: Human
  • Human, 3 human kidney donors, age 20, 23, 25
Gugen-Guillouzo, C., Campion, J., Brissot, P., Glaise, D., Launois, B., Bourel, M., and Guillouzo, A.: High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver , Cell Biol Int Rep 6 (6), 625, 1982
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     HEPES


Liver: Human
  • Human, 51-75 years old
    (also rat, guinea-pig, rabbit, dog)
Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 90 u/ml
Medium     CF EGTA perfusate


Liver: Human
  • Human
Kaighn, M.: Human Liver Cells,Tissue Culture Methods / Applications,(Kruse, P., Patterson, M. eds), , 54, 1973
 
Cells:      Hepatocytes
Enzyme(s)     Trypsin: 0.1%
Medium     HBSS, CMF


Liver: Monkey
  • Monkey, Macaca mulatta, 3-5.5 kg
Weber, A., Groyer-Picard, M.and Dagher, I.: Hepatocyte Transplantation Techniques: Large Animal Models. , Methods Mol Biol 481, 83, 2009
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 129 u/ml
Medium     DMEM/F12


Liver: Monkey
  • Monkey (Macaca fascicularis), adult, 5-6 Kg
Ulrich, R., Aspar, D., Cramer, C., Kletzien, R., and Ginsberg, L.: Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis) , In Vitro Cell Dev Biol 26, 815, 1990
 
Cells:      Hepatocytes
Enzyme(s)     Trypsin: 160 u/ml
Medium     HEPES buffer


Liver: Mouse
  • Mouse
Cabral, F., Miller, C., Kudrna, K., Hass, B., Daubendiek, J., Kellar, B. and Harris, E.: Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. , J Vis Exp , , 2018
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Medium     DMEM


Liver: Mouse
  • Mouse, 10-13 week
Sewing, S., Boess, F., Moisan, A., Bertinetti-Lapatki, C., Minz, T., Hedtjaern, M., Tessier, Y., Schuler, F., Singer, T. and Roth, A.: Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs. , PLoS ONE 11, e0159431, 2016
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.2 u/ml
Medium     William's Medium E


Liver: Mouse
  • Mouse
Weerasinghe, S., Park, M., Portney, D. and Omary, M.: Mouse Genetic Background Contributes to Hepatocyte Susceptibility to Fas-mediated Apoptosis. , Mol Biol Cell 27, 3005-3012, 2016
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 150 u/ml
Medium     PBS


Liver: Mouse
  • Mouse
Bigorgne, A., John, B., Ebrahimkhani, M., Shimizu-Albergine, M., Campbell, J. and Crispe, I.: TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota. , PLoS ONE 11, e0151063, 2016
 
Cells:      Hepatic
Enzyme(s)     Collagenase Type 4: 0.05%
Medium     HBSS


Liver: Mouse
  • Mouse, 12 week
Mederacke, I., Dapito, D., Affo, S., Uchinami, H. and Schwabe, R.: High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers. , Nat Protoc 10, 305-15, 2015
 
Cells:      Hepatic stellate
Enzyme(s)     Collagenase Type 4: 0.05%
Pronase: 0.04%
Medium     DMEM


Liver: Mouse
  • Mouse
Guenthner, C., Luitje, M., Pyle, L., Molyneux, P., Yu, J., Li, A., Leise, T. and Harrington, M.: Circadian Rhythms of Per2::Luc in Individual Primary Mouse Hepatocytes and Cultures. , PLoS ONE 9, e87573, 2014
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 3: 100 u/ml
Collagenase Type 4: 100 u/ml
Medium     DMEM


Liver: Mouse
  • Mouse
Sin, Y., Ballantyne, L., Mukherjee, K., St Amand, T., Kyriakopoulou, L., Schulze, A. and Funk, C.: Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism. , PLoS ONE 8, e80001, 2013
 
Cells:      hepatocytes
Enzyme(s)     Collagenase Type 4: 100 u/ml
Medium     DMEM


Liver: Mouse
  • Mouse
Perepelyuk, M., Terajima, M., Wang, A., Georges, P., Janmey, P., Yamauchi, M. and Wells, R.: Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. , Am J Physiol Gastrointest Liver Physiol 304, G605, 2013
 
Cells:      Hepatocytes and sinusoidal endothelial
Enzyme(s)     Collagenase Type 4: 0.05%
Deoxyribonuclease I: 0.003%
Medium     HBSS


Liver: Mouse
  • Mouse, female, 8-10 week
Severgnini, M., Sherman, J., Sehgal, A., Jayaprakash, N., Aubin, J., Wang, G., Zhang, L., Peng, C., Yucius, K., Butler, J. and Fitzgerald, K.: A Rapid Two-Step Method for Isolation of Functional Primary Mouse Hepatocytes: Cell Characterization and Asialoglycoprotein Receptor Based Assay Development. , Cytotechnology 64, 187-95, 2012
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.08%
Medium     DMEM


Liver: Mouse
  • Mouse
Brundert, M., Heeren, J., Merkel, M., Carambia, A., Herkel, J., Groitl, P., Dobner, T., Ramakrishnan, R., Moore, K. and Rinninger, F.: Scavenger Receptor CD36 Mediates Uptake of High Density Lipoproteins in Mice and by Cultured Cells. , J Lipid Res 52, 745, 2011
 
Cells:      Hepatocytes & non-parenchymal liver cells
Enzyme(s)     Collagenase: 0.03-0.05%
Medium     DMEM


Liver: Mouse
  • Mouse
Kang, H., Okamoto, K., Kim, Y., Takeda, Y., Bortner, C., Dang, H., Wada, T., Xie, W., Yang, X., Liao, G. and Jetten, A.: Nuclear Orphan Receptor TAK1/TR4-Deficient Mice are Protected Against Obesity-Linked Inflammation, Hepatic Steatosis, and Insulin Resistance. , Diabetes 60, 177, 2011
 
Cells:      Hepatocytes
Enzyme(s)     HIS kit: with modifications
Medium     DMEM


Liver: Mouse
  • Mouse
Holl, D., Kuckenberg, P., Woynecki, T., Egert, A., Becker, A., Huss, S., Stabenow, D., Zimmer, A., Knolle, P., Tolba, R., Fischer, H. and Schorle, H.: Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia. , PLoS ONE 6, e22034, 2011
 
Cells:      Hepatacytes
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     Williams E


Liver: Mouse
  • Mouse
Rountree, C., Ding, W., Dang, H., Vankirk, C. and Crooks, G.: Isolation of CD133+ Liver Stem Cells for Clonal Expansion. , J Vis Exp 56, e3183, 2011
 
Cells:      CD133+ stem
Enzyme(s)     Collagenase Type 4: 0.05%
Pronase: 0.05%
Deoxyribonuclease I: 0.01%
Medium     DMEM/F12


Liver: Mouse
  • Mouse, male, 12-14 week
Chung, S., Timmins, J., Duong, M., Degirolamo, C., Rong, S., Sawyer, J., Singaraja, R., Hayden, M., Maeda, N., Rudel, L., Shelness, G. and Parks, J.: Targeted Deletion of Hepatocyte ABCA1 Leads to VLDL Triglyceride Over-Production and LDL Hypercatabolism , J Biol Chem 285, 12197, 2010
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.03%
Medium     Williams E


Liver: Mouse
  • Mouse
Bosschaerts, T., Guilliams, M., Stijlemans, B., Morias, Y., Engel, D., Tacke, F., Herin, M., De Baetselier, P. and Beschin, A.: Tip-DC Development During Parasitic Infection is Regulated by IL-10 and Requires CCL2/CCR2, IFN-gamma and MyD88 Signaling. , PLoS Pathog 6, e1001045, 2010
 
Cells:      Liver non-parenchymal
Enzyme(s)     Collagenase Type 3: 100 u/ml
Medium     HBSS


Liver: Mouse
  • Mouse
Shmarakov, I., Fleshman, M., D'Ambrosio, D., Piantedosi, R., Riedl, K., Schwartz, S., Curley, R., von Lintig, J., Rubin, L., Harrison, E. and Blaner, W.: Hepatic Stellate Cells are an Important Cellular Site for β-Carotene Conversion to Retinoid. , Arch Biochem Biophys 504, 3-10, 2010
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.075%
Medium     HBSS


Liver: Mouse
  • Mouse, male, adult
Mathijs, K., Kienhuis, A., Brauers, K. J, Jennen, D. , Lahoz, A., Kleinjans, J. and van Delft, J.: Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes. , Drug Metab Dispos 37, 1305, 2009
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Medium     HBSS


Liver: Mouse
  • Mouse, male
Oliva, J., Bardag-Gorce, F., French, B., Li, J., McPhaul, L., Amidi, F., Dedes, J., Habibi, A., Nguyen, S. and French, S.: Fat10 is an Epigenetic Marker for Liver Preneoplasia in a Drug-Primed Mouse Model of Tumorigenesis. , Exp Mol Pathol 84, 102, 2008
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 100 u/ml
Elastase: 0.1 u/ml
Medium     Williams E


Liver: Mouse
  • Mouse, 7-10 week
Follenzi, A., Benten, D., Novikoff, P., Faulkner, L., Raut, S. and Gupta, S.: Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice. , J Clin Invest 118, 935, 2008
 
Cells:      Liver endothelial
Enzyme(s)     Collagenase: 0.03%
Medium     DMEM


Liver: Mouse
  • Mouse, 10-12 week
Beldi, G., Wu, Y., Sun, X., Imai, M., Enjyoji, K., Csizmadia, E., Candinas, D., Erb, L. and Robson, S.: Regulated Catalysis of Extracellular Nucleotides by Vascular CD39/ENTPD1 is Required for Liver Regeneration. , Gastroenterol 135, 1751, 2008
 
Cells:      Liver sinusoidal endothelial cells
Enzyme(s)     Collagenase Type 1: 0.05%
Neutral Protease: 0.025%
Medium     HEPES


Liver: Mouse
  • Mouse
Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse , Stem Cells 24, 322-32, 2006
 
Cells:      Liver epithelial progenitor cells
Enzyme(s)     Collagenase Type 4: 0.1%
Deoxyribonuclease I: 0.05%
Medium     DMEM


Liver: Mouse
  • Mouse
Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice , Hepatol 42, 140-8, 2005
 
Cells:      Liver sinusodial endothelial
Enzyme(s)     Collagenase: 0.03%
Medium     DMEM


Liver: Mouse
  • Mouse
Jiang Guoqiang, Li Zhihua, Liu Franklin, Ellsworth Kenneth, Dallas-Yang Qing, Wu Margaret, Ronan John, Esau Christine, Murphy Cain, Szalkowski Deborah, Bergeron Raynald, Doebber Thomas, Zhang Bei: Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1 , J Clin Invest 115, 1030-8, 2005
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.033%
Medium     Leffert's buffer


Liver: Mouse
  • Mouse, 8-10 week
Kotton, D., Fabian, A. and Mulligan, R.: A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity. , Blood 106, 1574, 2005
 
Cells:      Liver derived stem cells
Enzyme(s)     Collagenase Type 1: 0.1%
Neutral Protease: 2.4 u/ml
Medium     HBSS


Liver: Mouse
  • Mouse, male, 8 week
Hatano E, Brenner DA: Akt protects mouse hepatocytes from TNF-alpha- and Fas-mediated apoptosis through NK-kappa B activation. , Am J Physiol/Gastro 282, G1357, 2002
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.04%
Medium     Waymouth's medium


Liver: Mouse
  • Mouse
Sazani P., Gemignani F., Kang S., Maier M., Manoharan M., Persmark M., Bortner D., and Kole R.: Systemically Delivered Antisense Oligomers Up-regulate Gene Expression in Mouse Tissues , Nat Biotechnol 20, 1228, 2002
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.053%
Medium     DMEM/ F-12


Liver: Mouse
  • Mouse, male, 4-10 week
Lingohr Melissa K, Bull Richard J, Kato-Weinstein Junko, Thrall Brian D: Dichloroacetate stimulates glycogen accumulation in primary hepatocytes through an insulin-independent mechanism , Toxicol Sci 68, 508-15, 2002
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 100 u/ml
Medium     HBSS


Liver: Mouse
  • Mouse
Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers , Drug Metab Dispos 28, 1321-6, 2000
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     Williams E


Liver: Mouse
  • Mouse, 7 week
Angele MK, Knoferl MW, Schwacha MG, Ayala A, Cioffi WG, Bland KI, and Chaudry IH.: Sex steroids regulate pro- and anti-inflammatory cytokine release by macrophages after trauma-hemorrhage , Am J Physiol 277, C35, 1999
 
Cells:      Kupffer cells
Enzyme(s)     Collagenase Type 4: 0.05%
Medium     HBSS


Liver: Mouse
  • Mouse
Ling W, Lougheed M, Suzuki H, Buchan A, Kodama T, Steinbrecher UP: Oxidized or acetylated low density lipoproteins are rapidly cleared by the liver in mice with disruption of the scavenger receptor class A type I/II gene , J Clin Invest 100, 244-52, 1997
 
Cells:      Nonparenchymal liver
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     Hanks


Liver: Mouse
  • Mouse, C3H, 6-8 weeks
Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver , In Vitro 15, 910, 1979
 
Cells:      Epithelial
Enzyme(s)     Collagenase: 0.10%
Medium     DMEM


Liver: Mouse
  • Mouse, 20-30 g
Crisp, D., and Pogson, C.: Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver , Biochem J 126, 1009, 1972
 
Cells:      Parenchymal and non-parenchymal
Enzyme(s)     Hyaluronidase: 0.1%
Medium     Hank's w/ Insulin, CMF


Liver: Porcine
  • Porcine, mini-pig, 13 kg
Meng, F., Chen, Z., Han, M., Hu, X., He, X., Liu, Y., He, W., Huang, W., Guo, H. and Zhou, P.: Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination. , World J Gastroenterol 16, 1660, 2010
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Neutral Protease: 0.84%
Deoxyribonuclease I: see reference
Medium     Williams E


Liver: Porcine
  • Porcine, Yorkshire, male, 21 day
Terner, M., Gilmore, W.J., Lou, Y. and Squires, E.J.: The Role of CYP2A and CYP2E1 in the Metabolism of 3-Methylindole in Primary Cultured Porcine Hepatocytes. , Drug Metab Dispos 34, 848, 2006
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.07%
Medium     Williams E


Liver: Porcine
  • Porcine, Chinese mini pig, 6-10 kg
Li, J, Li, L., Chao, H., Yang, Q., Liu, X., Sheng, J., Yu, H. and Huang, J: Isolation and Short Term Cultivation of Swine Hepatocytes for Bioartificial Liver Support System. , Hepatobiliary Pancreat Dis Int 4, 249, 2005
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 125 u/ml
Medium     Williams E


Liver: Porcine
  • Porcine, one week
Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich , World J Gastroenterol 10, 699, 2004
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Medium     RPMI 1640


Liver: Porcine
  • Porcine, male, 2-3 week
Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures , Am J Physiol Regul Integr Comp Physiol 286, R206-16, 2004
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     DMEM


Liver: Porcine
  • Porcine, adult
Zhou X, Liu L, Kano J, Mukaiyama T, and Tokiwa T.: Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System , Chin Med J 114, 946, 2001
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Neutral Protease:
Medium     MEM


Liver: Porcine
  • Porcine, male, 6-40 kg
Gerlach, J., Brombacher, J., Smith, M., Neuhaus, P.: High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight , J Surg Res 62 (1), 85, 1996
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.8%
Medium     PBS


Liver: Rabbit
  • Rabbit, New Zealand white
Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 90 u/ml
Medium     CF EGTA perfusate


Liver: Rat
  • Rat, SD, 500-700g
Perepelyuk, M., Terajima, M., Wang, A., Georges, P., Janmey, P., Yamauchi, M. and Wells, R.: Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. , Am J Physiol Gastrointest Liver Physiol 304, G605, 2013
 
Cells:      Hepatocytes and sinusoidal endothelial
Enzyme(s)     Collagenase Type 4: 0.05%
Deoxyribonuclease I: 0.003%
Medium     HBSS


Liver: Rat
  • Rat, adult
Shen, L., Hillebrand, A., Wang, D. and Liu, M.: Isolation and Primary Culture of Rat Hepatic Cells. , J Vis Exp 64, e3917, 2012
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 1000 U
Medium     William's


Liver: Rat
  • Rat, adult
Wen, J., Olsen, A., Perepelyuk, M. and Wells, R.: Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion. , J Vis Exp 64, e3669, 2012
 
Cells:      Portal fibroblasts
Enzyme(s)     Collagenase Type 2: 0.3%
Medium     DMEM/F-12


Liver: Rat
  • Rat
Budick-Harmelin, N., Anavi, S., Madar, Z. and Tirosh, O.: Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes. , Lipids Health Dis 11, 66, 2012
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     DMEM


Liver: Rat
  • Rat, SD, 240-320g
Pillai, V., and Mehvar, R.: Inhibition of NADPH-Cytochrome P450 Reductase by Tannic Acid in Rat Liver Microsomes and Primary Hepatocytes: Methodological Artifacts and Application to Ischemia-Reperfusion Injury , J Pharm Sci 100, 3495, 2011
 
Cells:      Hepatocytes
Enzyme(s)     HIS kit: per instructions
Medium     Waymouth's MB


Liver: Rat
  • Rat
Gopalakrishnan, S. and Harris, E.: In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion. , J Vis Exp 57, e3138, 2011
 
Cells:      Hepatocytes and SEC's
Enzyme(s)     Collagenase Type 1: 0.1-0.2%
Medium     RPMI


Liver: Rat
  • Rat, Wistar, female
Zvibel, I., Atias, D., Phillips, A., Halpern, Z. and Oren, R.: Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho. , Lab Invest 90, 674, 2010
 
Cells:      Hepatic stellate cells
Enzyme(s)     Collagenase Type 1: 0.025-0.1%
Pronase: 0.025-0.13%
Medium     DMEM


Liver: Rat
  • Rat, SD
Handy, J., Saxena, N., Fu, P., Lin, S., Mells, J., Gupta, N. and Anania, F.: Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3). , J Cell Biochem 110, 1195, 2010
 
Cells:      Hepatic stellate cells
Enzyme(s)     Pronase: 0.02%
Collagenase: see reference
Medium     Medium 199


Liver: Rat
  • Rat, SD, 250-300g
Parasrampuria, R. and Mehvar, R.: Dose-Dependent Inhibition of Transporter-Mediated Hepatic Uptake and Biliary Excretion of Methotrexate by Cyclosporine A in an Isolated Perfused Rat Liver Model. , J Pharm Sci 99, 5060, 2010
 
Cells:      Hepatocytes
Enzyme(s)     HIS kit: per instructions
Medium     Krebs-Henseleit


Liver: Rat
  • Rat, SD
Chung, C., Shugrue, C., Nagar, A., Doll, J., Cornwell, M., Gattu, A., Kolodecik, T., Pandol, S. and Gorelick, F.: Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator. , Gastroenterol 136, 331, 2009
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     HBSS


Liver: Rat
  • Rat, Lewis, male
Bettinger, C., Kulig, K., Vacanti, J., Langer, R. and Borenstein, J.: Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture. , Tissue Eng Part A 15, 1321, 2009
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     DPBS


Liver: Rat
  • Rat, 200-300 g
Mula, N., Cubero, F., Codesal, J., de Andres, S., Escudero, C., Garcia-Barrutia, S., Millan, I., Arahuetes, R. and Maganto, P.: Survival of Allogeneic Hepatocytes Transplanted into the Thymus. , Cells Tissues Organs 188, 270, 2008
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.067%
Medium     HBSS


Liver: Rat
  • Rat, SD, male, 230-250g
Doleh, L. and Romani, A.: Biphasic Effect of Extra-Reticular Mg2+ on Hepatic G6P Transport and Hydrolysis. , Arch Biochem Biophys 467, 283, 2007
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.1%
Medium     HEPES


Liver: Rat
  • Rat, Lewis, 150-200g
Smith, M. and Mooney, D.: Hypoxia Leads to Necrotic Hepatocyte Death. , J Biomed Mater Res A 80, 520, 2007
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     Williams E


Liver: Rat
  • Rat, SD, 180-200g
Charbonneau, A., Unson, C. and Lavoie, J.: High-Fat Diet-Induced Hepatic Steatosis Reduces Glucagon Receptor Content in Rat Hepatocytes: Potential Interaction with Acute Exercise. , J Physiol 579, 255, 2007
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     see reference


Liver: Rat
  • Rat, Wistar, 270-330g
Li, W., Ralphs, K., Slack, J. and Tosh, D.: Keratinocyte Serum-Free Medium Maintains Long-Term Liver Gene Expression and Function in Cultured Rat Hepatocytes by Preventing the Loss of Liver-Enriched Transcription Factors. , Int J Biochem Cell Biol 39, 541-54, 2007
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.033%
Medium     Williams E


Liver: Rat
  • Rat, SD, 500-750 g
Li, Z., Dranoff, J., Chan, E., Uemura, M., Sevigny, J.and Wells, R.: Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. , Hepatol 46, 1246-56, 2007
 
Cells:      Portal fibroblasts
Enzyme(s)     Collagenase: 0.03%
Pronase: 0.033%
Hyaluronidase: 0.036%
Medium     DMEM/F-12


Liver: Rat
  • Rat, Fisher, E14
Isabel Zvibel, Miri Bronstein, Einav Hubel, Ella Bar-Lev, Zamir Halpern, Ran Oren: Isolation, characterization and culture of Thy1-positive cells from fetal rat livers , World J Gastroenterol 12, 3841-7, 2006
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.22%
Medium     HBSS


Liver: Rat
  • Rat, Lewis, female, 150-200 g
Sosef MN, Baust JM, Sugimachi K, Fowler A, Tompkins RG, and Toner M.: Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function , Ann Surg 241, 125, 2005
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: see references
Medium     DMEM


Liver: Rat
  • Rat
Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice , Hepatol 42, 140-8, 2005
 
Cells:      Liver sinusodial endothelial
Enzyme(s)     Collagenase: 0.03%
Medium     DMEM


Liver: Rat
  • Rat, Wistar, 200-300 g
Putz G, Schmider W, Nitschke R, Kurz G, Blum HE: Synthesis of phospholipid-conjugated bile salts and interaction of bile salt-coated liposomes with cultured hepatocytes , J Lipid Res 46, 2325-38, 2005
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     Williams E


Liver: Rat
  • Rat, adult
Jensen C., Jauho E., Santoni-Rugiu E., Holmskov U., Teisner B., Tygstrup N., and Bisgaard H.: Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 , Am J Pathol 164(4), 1347, 2004
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Pronase: 1%
Deoxyribonuclease I: 0.007%
Medium     Williams E


Liver: Rat
  • Rat, Wistar
Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich , World J Gastroenterol 10, 699, 2004
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Medium     RPMI 1640


Liver: Rat
  • Rat, SD, male, 200-250 g
Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures , Am J Physiol Regul Integr Comp Physiol 286, R206-16, 2004
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     DMEM


Liver: Rat
  • Rat, Wistar, 220-270g
Annaert, P., Turncliff, R., Booth, C., Thakker, D. and Brouwer, K.: P-Glycoprotein-Mediated In Vitro Biliary Excretion in Sandwich-Cultured Rat Hepatocytes. , Drug Metab Dispos 29, 1277-83, 2001
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 200 u/ml
Medium     DMEM


Liver: Rat
  • Rat, SD, male
Burczynski, M., McMillian, M., Parker, J., Bryant, S., Leone, A., Grant, E., Thorne, J., Zhong, Z., Zivin, R. and Johnson, M.: Cytochrome P450 Induction in Rat Hepatocytes Assessed by Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction and the RNA Invasive Cleavage Assay. , Drug Metab Dispos 29, 1243, 2001
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.025%
Medium     Williams E


Liver: Rat
  • Rat, SD, male, 230-250 g
Kuddus, R., Nalesnik, M., Subbotin, V., Rao, A., and Gandhi, C.: Enhanced Sythesis and Reduced Metabolism of Endothelin-1 (ET-1) by Hepatocytes - An Important Mechanism of Increased Endogenous Levels of ET-1 in Liver Cirrhosis , J Hepatol 33, 725, 2000
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.1%
Medium     HBSS


Liver: Rat
  • Rat, male, 3 week
Low-Baselli A., Hufnagl K., Parzefall W., Schulte-Hermann R., and Grasl-Kraupp B.: Initiated Rat Hepatocytes in Primary Culture: A Novel Tool to Study Alterations in Growth Control During the First Stage of Carcinogenesis , Carcinogenesis 21, 79, 2000
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.02%
Medium     Williams E


Liver: Rat
  • Rat, SD, male, 450-500 g
Gabriel, A., Kuddus, R., Rao, A., and Gandhi, C.: Down-Regulation of Endothelin Receptors by Transforming Growth Factor B1 in Hepatic Stellate Cells , J Hepatol 30, 440, 1999
 
Cells:      Stellate
Enzyme(s)     Protease: 0.02%
Medium     HBSS


Liver: Rat
  • Rat, Wistar, male
Wolz E, Liechti H, Notter B, Oesterhelt G, Kistler A: Characterization of metabolites of astaxanthin in primary cultures of rat hepatocytes , Drug Metab Dispos 27, 456-62, 1999
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 120 u/ml
Medium     HBSS


Liver: Rat
  • Rat, Lewis, adult
Matsuura T, Gad MZ, Harrison EH, and Ross AC: Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocyte and nonparenchymal cell fractions of rat liver , J Nutr 127, 218, 1997
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 60 u/ml
Medium     HEPES


Liver: Rat
  • Rat
Alston-Smith, J and Pertoft, H: Isolation of Liver Cells: a System for Obtaining Pure Cells in Monolayer Cultures from a Single Rat Liver,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:14.1, 1995
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.04% - 0.06%
Medium     PBS


Liver: Rat
  • Rat
Matsumoto, K and Nakamura, T: Techniques for the Isolation and Cultivation of Hepatocytes using Collagenase,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:16.1, 1995
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     DMEM


Liver: Rat
  • Rat, SD, male, 200 g
Rana, B., Mischoulon, D., Xie, Y., Bucher, N., and Farmer, S.: Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors , Mol Cell Biol 14 (9), 5858, 1994
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     Medium 199


Liver: Rat
  • Rat, Wistar, adult, male, 100-125 g
Liu, J., McKim, J., Liu, Y., and Klaassen, C.: Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures , In Vitro Cell Dev Biol 28, 320, 1992
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     Serum-free medium


Liver: Rat
  • Rat, SD, male, 250-350 g
Dixit, V., Arthur, M., and Gitnick, G.: Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats , Cell Transplant 1, 275, 1992
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 200 u/ml
Medium     RPMI 1640


Liver: Rat
  • Rat, Fisher
Blouin, M., and Marceau, N.: Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells, J Tiss Cul Meth 13, 117, 1991
 
Cells:      Epithelial
Enzyme(s)     Collagenase: 0.05%
Neutral Protease: 0.48%
Hyaluronidase: 0.075%
Medium     Eagles's MEM


Liver: Rat
  • Rat, SD, 8 - 12 day
Davila, J., Reddy, C., Davis, P. and Acosta, D.: Toxicity Assessment of Papaverine Hydrochloride and Papaverine-Derived Metabolites in Primary Cultures of Rat Hepatocytes , In Vitro Cell Dev Biol 26, 515, 1990
 
Cells:      Parenchymal hepatocytes
Enzyme(s)     Collagenase Type 4: 80 u/ml
Medium     HBSS


Liver: Rat
  • Rat, SD, male, 250-350 g
Liu, J., Kershaw, W., and Klaassen, C.: Rat Primary Hepatocyte Cultures are a Good Model for Examining Metallothionein-Induced Tolerance to Cadmium Toxicity , In Vitro Cell Dev Biol 26, 75, 1990
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     HBSS, CMF


Liver: Rat
  • Rat, Wistar, male, 200 g
Kindberg, G., Gudmundsen, O., and Berg, T.: The Effect of Vandate on Receptor-mediated Endocytosis of Asialoorosomucoid in Rat Liver Parenchymal Cells , J Biol Chem 265, 8999, 1990
 
Cells:      Parenchymal
Enzyme(s)     Collagenase Type 1:
Medium     HEPES, modified


Liver: Rat
  • Rat, SD, male, 175-225 g
Reddy, S., Amick, G., Cooper, R., and Damun, Z.: Insulin Stimulates the Activity of a Protamine in Isolated Rat Hepatocytes , J Biol Chem 265, 7748, 1990
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase:
Medium     Kreb's Ringer bicarbonate buffer


Liver: Rat
  • Rat, SD, neonatal, 8 - 10 days
Davila, J., Lenherr, A., and Acosta, D.: Protective Effect of Flavonoids on Drug-Induced Hepatotoxicity In Vitro , Toxicology 57, 267, 1989
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 0.05%
Medium     Hanks' BSS, CF


Liver: Rat
  • Rat, Fisher, male, 125-250 g
Chan, K., Kost, D., and Michalopoulos, G.: Multiple Sequential Periods of DNA Synthesis and Quiescence in Primary Hepatocyte Cultures Maintained on the DMSO-EGF On/Off Protocol , J Cell Physiol 141, 584, 1989
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.5%
Medium     RPMI 1640


Liver: Rat
  • Rat, Fischer 344, male, 14 months
Nagy, I., Ohno-Iwashita, Y., Ohta, M., Nagy, V., Kitani, K., Ando, S., and Imahori, K.: Effect of Perfringolysin O on the Lateral Diffusion Constant of Membrane Proteins of Hepatocytes as Revealed by Fluorescence Recovery After Photobleaching , Biochim Biophys Acta 939, 551, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     Ringer's biocarbonate buffer


Liver: Rat
  • Rat, SD, male, 200-300 g
Voss, A. and Sprecher, H.: Metabolism of 6,9,12-Octadecatetraenoic and 6,9,12,15-Octadecatetraenoic Acid , Biochim Biophys Acta 958, 153, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     HEPES


Liver: Rat
  • Rat, Wistar, male,3 month, 200 g
Kuiper, J., Zijlstra, F., Kamps, J, and vanBerkel, T.: Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells , Biochim Biophys Acta 959, 143, 1988
 
Cells:      Parenchymal
Endothelial
Kupffer
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     Krebs Henseleit


Liver: Rat
  • Rat, Wistar, male, 200 - 300 g
Cai, H., He, Z., and Ding, Y.: Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors , Biochim Biophys Acta 958, 334, 1988
 
Cells:      Parenchymal
Kupffer
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     HBSS with CaCl2


Liver: Rat
  • Rat, Wistar, female, 17 days
Cotariu, D., Barr-Nea, L., Papo, N., and Zaidman, J.: Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes , Enzyme 40, 212, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     HBSS


Liver: Rat
  • Rat, CD strain, albino, male, 140 -180 g
Braun, L., Mead, J., Panzica, M., Mikumo, R., Bell, G., and Fausto, N.: Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation , Proc Natl Acad Sci U S A 85, 1539, 1988
 
Cells:      Hepatocytes and Nonparenchymal
Enzyme(s)     Pronase: 0.1%
Medium     HEPES buffer with calcium


Liver: Rat
  • Rat, 250-350 g
    (also guinea-pig & rabbit)
Chang, T., and Chang, C.: Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution , Biochim Biophys Acta 942, 57, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 166 u/ml
Medium     DMEM


Liver: Rat
  • Rat, SD, male, 180-200 g
Gupta, C., Hattori, A., Betschart, J., Virji, M., and Shinozuka, H.: Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet , Cancer Res 48, 1162, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     MEM


Liver: Rat
  • Rat, Fisher 344, 150-200 g
Mathis, G., Walls, S., and Sirica, A.: Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes , Cancer Res 48, 6145, 1988
 
Cells:      Bile ductular epithelial
Enzyme(s)     Collagenase Type 1: 220 u/ml
Medium     L-15


Liver: Rat
  • Rat, Wistar, male, 260-310 g
McAbee DD and Weigel PH: ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes , Biochemistry 27, 2061, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     HEPES


Liver: Rat
  • Rat, SD, male, 350-400 g
Rodriguez de Turco, E., and Spitzer, J.: Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats , Biochem J 253, 73, 1988
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2:
Medium     HBSS


Liver: Rat
  • Rat, SD, male, 350 - 450 g
Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan , Anal Biochem 161, 207, 1987
 
Cells:      Lipocytes
Kupffer
Sinusoidal endothelial
Enzyme(s)     Collagenase: 0.015%
Medium     DMEM/Ham's F-12


Liver: Rat
  • Rat, SD, male
Oka, J. and Weigel, P.: Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes , J Cell Physiol 133, 243, 1987
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 100 - 200 µg/g body weight
Medium     Eagle's
Eagle's w/HEPES
HBSS


Liver: Rat
  • Rat, Fischer, male, 150-250 g
Francavilla, A., Ove, P., Polimeno, L., Sciascia, C., Coetzee, M., and Starzi, T.: Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy , Cancer Res 46, 1318, 1986
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 125 - 250 u/ml
Medium     MEM
(see reference)


Liver: Rat
  • Rat, SD, male, 200-250 g
Kreamer, B., Staecker, J., Sawada, N., Sattler, G., Hsia, M., and Pitot, H.: Use of Low-Speed, Iso-Density Percoll Centrifugation Method to Increase the Viability of Isolated Rat Hepatocyte Preparations , In Vitro Cell Dev Biol 22 (4), 201, 1986
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     HBSS


Liver: Rat
  • Rat, SD, female, 100-180 g
Schwarz, K., Lanier, S., Carter, E., Homcy, C., and Graham, R.: Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture , Mol Pharmacol 27, 200, 1985
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.5%
Medium     Krebs Ringer bicarbonate buffer


Liver: Rat
  • Rat, Lewis, female, 200-250 g
Holstege A, Leser HG, Pausch J, Gerok W: Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes , Eur J Biochem 149, 169-73, 1985
 
Cells:      Hepatocytes, Kupffer, endothelial
Enzyme(s)     Collagenase: 0.05%
Medium     Gey's BSS


Liver: Rat
  • Rat, SD, male, 270 - 320 g
Brass, E., Garrity, M., and Robertson, R.: Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins , F.E.B.S. Lett. 169, 293, 1984
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.04%
Medium     Bicarbonate buffer with calcium added


Liver: Rat
  • Rat, Wistar, adult, male, 150-200 g
Okumura, T. and Saito, K.: Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes , J. Biochem. 96, 429, 1984
 
Cells:      Hepatocytes
Enzyme(s)     Trypsin: 0.005%
Medium     Williams E


Liver: Rat
  • Rat, Fischer, Lewis and SD, male 10-18 months
Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats , In Vitro 19, 576, 1983
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.05%
Medium     HBSS CMF


Liver: Rat
  • Rat, Wistar, male, 3 month old
Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells , J Biol Chem 258, 12221, 1983
 
Cells:      Endothelial
Kupffer
Parenchymal
Enzyme(s)     Collagenase: 0.05%
Pronase: 0.25%
Medium     HBSS


Liver: Rat
  • Rat, SD, male/female, 250-300 g
Studer, R. and Borle, A.: Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines , J Biol Chem 257, 7987, 1982
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 100 u/ml
Medium     Krebs Henseleit bicarbonate buffer


Liver: Rat
  • Rat, Wistar, 12 day
Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels , Exp Cell Res 131, 363, 1981
 
Cells:      Epithelial
Enzyme(s)     Trypsin: 0.05%
Medium     HBSS, CMF


Liver: Rat
  • Rat, Zucker, obese
Goldstein, A., Palmer, J., and Johnson, P.: Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis , In Vitro 17, 651, 1981
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 2: 0.30%
Medium     Dulbecco-Vogt arginine free Eagle's


Liver: Rat
  • Rat, SD
Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies , In Vitro 17, 935, 1981
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 90 u/ml
Medium     CF EGTA perfusate


Liver: Rat
  • Rat, Wistar, female, fetus
Gugen-Guillouzo, C., Tichonicky, L., Szajnert, M., and Kruh, J.: Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes , In Vitro 16, 1, 1980
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.025%
Medium     HEPES buffer


Liver: Rat
  • Rat, SD, male, 250-300 g
Yamada, S., Otto, P., Kennedy, D., and Whayne, T.: The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture , In Vitro 16, 559, 1980
 
Cells:      Parenchymal
Enzyme(s)     Collagenase Type 2: 0.05%
Medium     Kreb's Henseleit bicarbonate buffer


Liver: Rat
  • Rat, Wistar, male, 180-210 g
De Gerlache, J., Lans, M., Taper, H., and Roberfroid, M.: Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization , Toxicology 18, 225, 1980
 
Cells:      Hepatocytes
Enzyme(s)     Hyaluronidase: 0.02%
Medium     Kreb's buffer


Liver: Rat
  • Rat, SD, 7-10 day
Acosta, D., Anuforo, D., and Smith, R: Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells, J Tiss Cul Meth 6, 35, 1980
 
Cells:      Liver
Enzyme(s)     Collagenase: 0.05%
Medium     HBSS modified (see reference)


Liver: Rat
  • Rat, Wistar, male, 200-250 g
Poli, G., Gravela, E., Albano, E., and Dianzani, M.: Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion , Exp Mol Pathol 30, 116, 1979
 
Cells:      Hepatocytes
Enzyme(s)     Hyaluronidase: 460 u/ml
Medium     Saline


Liver: Rat
  • Rat, SD, female, 100-150 g
Davis, R., Engelhorn, S., Pangburn, S., Weinstein, D., and Steinberg, D.: Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes , J Biol Chem 254 (6), 2010, 1979
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.065%
Medium     DMEM
(see reference)


Liver: Rat
  • Rat
    (also chicken)
Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars , J Biol Chem 254 (21), 10830, 1979
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 4: 6000 units
Collagenase Type 3 & 4:
Medium     HEPES


Liver: Rat
  • Rat, SD, male, 200 g
Rubin, K., Kjellen, L., and Oslashbrink, B.: Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel , Exp Cell Res 109, 413, 1977
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 100 u/ml
Medium     Buffers 1 & 2
(see reference)


Liver: Rat
  • Rat (WAG/RIJ), female, 24, 30, and 37 months
VanBezodijen, C., Grell, T., and Knook, D.: Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells , Mech Ageing Dev 6, 293, 1977
 
Cells:      Parenchymal
Enzyme(s)     Collagenase Type 1: 0.05% - 0.06%
Medium     HEPES buffer


Liver: Rat
  • Rat, Fisher, adult, male
Williams, G., Bermudez, E., and Scaramuzzino, D.: Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium , In Vitro 13 (12), 809, 1977
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 100 u/ml
Medium     HBSS
(see reference)


Liver: Rat
  • Rat , Wistar, male, 250-300 g
Gravela, E., Poli, G., Albano, E., and Dianzani, M.: Studies of Fatty Liver with Isolated Hepatocytes , Exp Mol Pathol 27, 339, 1977
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%
Medium     Hank's solution, CF


Liver: Rat
  • Rat, Fischer, male, adult, 170-265 g
Laishes, B., and Williams, G.: Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin , In Vitro 12, 521, 1976
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase: 0.05%-0.10%
Medium     Williams E


Liver: Rat
  • Rat, SD, male, 200-250 g
Witters, L., Alberico, L., and Acruch, J.: Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte , Biochem Biophys Res Commun 69 (4), 997, 1976
 
Cells:      Hepatocytes
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     MEM


Liver: Rat
  • Rat, neonate, 3 day
Bausher, J., and Schaeffer, W.: A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1 , In Vitro 9, 286, 1974
 
Cells:      Hepatocytes
Enzyme(s)     Trypsin: 0.25%
Medium     PBS, CMF


Liver: Rat
  • Rat, SD, albino, male
Bonney, R., Becker, J., Walker, P., and Potter, V.: Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis , In Vitro 9, 399, 1974
 
Cells:      Parenchymal
Enzyme(s)     Hyaluronidase: 0.1%
Medium     Hank's solution, CMF


Liver: Rat
  • Rat, embryos, 1-3 days
Gerschenson, L., Berliner, J., and Davidson, M.: The Isolation and Culture of Liver Cells , Vol. 32,, , 733, 1974
 
Cells:      Hepatocytes
Enzyme(s)     Hyaluronidase: 0.10%
Medium     HBSS


Liver: Rat
  • Rat, Fischer, adult, 200-250 g
Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells , Exp Cell Res 89, 139, 1974
 
Cells:      Epithelial
Enzyme(s)     Hyaluronidase: 0.0075%
Medium     KCl-NaCl HEPES Buffer


Liver: Rat
  • Rat, SD, adult, male, 200-250 g
Howard, R., Lee, J., and Pesch, L.: The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques , J Cell Biol 57, 642, 1973
 
Cells:      Parenchymal
Enzyme(s)     Hyaluronidase: 0.10%
Medium     HBSS, CF


Liver: Rat
  • Rat, SD, adult, male, 180-300 g
Bissell, D., Hammaker, L., and Meyer, U.: Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies , J Cell Biol 59, 722, 1973
 
Cells:      Parenchymal
Enzyme(s)     Collagenase Type 1: 0.05%
Medium     Hank's solution, CF


Liver: Rat
  • Rat, Wistar, 200-250 g
Berg, T., Boman, D., and Seglen, P.O.: Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone , Exp Cell Res 72, 571, 1972
 
Cells:      Hepatocytes
Enzyme(s)     Hyaluronidase: 0.10%
Medium     HBSS, CF


Liver: Rat
  • Rat, SD, female, 130-160 g
Johnson, M., Das, N., Butcher, F., and Fain, J.: The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl C