Worthington Tissue Dissociation Guide

Dissociating Enzymes: Hyaluronidase

Hyaluronidase is a polysaccharidase with a specificity for endo-N-acetylhexosaminic bonds between 2-acetoamido-2-deoxy-beta-D-glucose and D-glucuronate. These bonds are common in hyaluronic acid and chondroitin sulfate A and C. Because these substances are found in high concentrations in the ground substance of virtually all connective tissues, hyaluronidase is often used for the dissociation of tissues, usually in combination with a crude protease such as collagenase.

More Information: Worthington Hyaluronidase

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Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat Adrenal Bone Brain
Cartilage Colon Endothelial Epithelial
Eye Heart Intestine Kidney
Liver Lung Lymph nodes Mammary
Miscellaneous Muscle Neural Pancreas
Parotid Pituitary Prostate Reproductive
Scales Skin Spleen Stem
Thymus Thyroid/Parathyroid Tonsil Tumor

Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.