Worthington Tissue Dissociation Guide

Dissociating Enzymes: Neutral Protease (Dispase)

Neutral Protease (Dispase) is a bacterial enzyme produced by Bacillus polymyxa that hydrolyses N-terminal peptide bonds of non-polar amino acid residues and is classified as an amino-endopeptidase. Its mild proteolytic action makes the enzyme especially useful for the isolation of primary and secondary (subcultivation) cells since it maintains cell membrane integrity.

Neutral Protease (Dispase) is also frequently used as a secondary enzyme in conjunction with collagenase and/or other proteases in many primary cell isolation and tissue dissociation applications. Neutral Protease (Dispase) dissociates fibroblast-like cells more efficiently than epithelial-like cells so it has also been used for differential isolation and culture applications. Other advantages are its non-mammalian (bacterial) source and its ability to be inhibited by EDTA.

More Information: Worthington Neutral Protease (Dispase)

Next: Trypsin Inhibitor (Soybean)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat Adrenal Bone Brain
Cartilage Colon Endothelial Epithelial
Eye Heart Intestine Kidney
Liver Lung Lymph nodes Mammary
Miscellaneous Muscle Neural Pancreas
Parotid Pituitary Prostate Reproductive
Scales Skin Spleen Stem
Thymus Thyroid/Parathyroid Tonsil Tumor

Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.